WO2021029455A1 - Heavy metal ion detection method and detection sensor based on ion-imprinted polymer - Google Patents

Abstract

Disclosed are a heavy metal ion detection method and a heavy metal ion detection sensor based on an ion-imprinted polymer, the method comprising the steps of: applying a heavy metal ion sample to a heavy metal ion detection sensor based on an ion-imprinted polymer to allow heavy metal ions to be adsorbed on the ion-imprinted polymer; adding a color development solution to the ion-imprinted polymer having the heavy metal ions adsorbed thereon to subject the ion-imprinted polymer having the heavy metal ions adsorbed thereon to color development; detecting the heavy metal ions by a color change of the color-developed ion-imprinted polymer; receiving an image of the color-developed ion-imprinted polymer; measuring RGB values of the color-developed ion-imprinted polymer from the received image; and calculating the concentration of the heavy metal ions on the basis of the measured RGB values.

Description

Heavy metal ion detection method and detection sensor based on ion imprinted polymer

The present invention relates to a heavy metal ion detection method and detection sensor based on an ion-imprinted polymer, and more particularly, a color change of the colored ion-imprinted polymer after adsorbing heavy metal ions to the ion-imprinted polymer in the heavy metal ion detection sensor A method for detecting heavy metal ions based on an ion imprinting polymer and a detection sensor for detecting heavy metal ions and measuring the concentration of heavy metal ions by

Sensors for the detection of heavy metal ions are becoming more important in various fields such as medicine, water quality, air, and food.

However, since the detection of heavy metal ions using conventional indicators is mostly performed in an aqueous solution state, it is difficult to selectively detect ions due to interfering substances present in the analysis sample.

In addition, in order to detect heavy metal ions by colorimetric method, not only does it require complicated processes such as pretreatment, but also expensive equipment is required to measure the concentration of detected heavy metal ions, so that the operator can easily check the concentration of heavy metal ions at the detection site. There was a problem that was difficult to do.

Accordingly, there is a need for a study on a detection method and a detection sensor capable of not only detecting heavy metal ions quickly and easily in the field through color change, but also measuring the concentration of heavy metal ions.

The present invention was conceived to solve the above problems, and an object of the present invention is to easily and easily detect heavy metal ions at work sites and measure the concentration of heavy metal ions without expensive detection equipment or pretreatment process. It is to provide a method and sensor for detecting heavy metal ions based on ion-imprinted polymer.

The method for detecting heavy metal ions based on an ion-imprinted polymer according to the present invention for achieving the above object comprises the steps of adsorbing heavy metal ions on the ion-imprinted polymer by adding a heavy metal ion sample to a heavy metal ion detection sensor based on the ion-imprinted polymer, Injecting a color developing solution into the adsorbed ion-imprinted polymer to color the ion-imprinted polymer with heavy metal ions adsorbed, detecting heavy metal ions by color change of the colored ion-imprinted polymer, and receiving an image of the colored ion-imprinted polymer The step of, measuring the RGB value of the colored ion-imprinted polymer from the received image, and calculating the concentration of heavy metal ions based on the measured RGB value.

The calculating of the concentration of the heavy metal ions includes calculating Euclidean distances (Eds) by correcting the measured RGB value as a reference RGB value, and the following equation based on the calculated Euclidean distance:

y = 0.6997x + 15.2566

y: Euclidean distance

x: concentration of heavy metal ions (mg/L)

It may include the step of calculating the concentration of heavy metal ions using.

In the method for detecting heavy metal ions based on the ion-imprinted polymer according to the present invention, after the step of calculating the concentration of the heavy metal ions, the calculated concentration of the heavy metal ions is displayed on the user terminal, or the calculated concentration of the heavy metal ions is It may further include transmitting to an external terminal.

The heavy metal ion is copper ion, zinc ion, cobalt ion, nickel ion, silver ion, cadmium ion, trivalent chromium ion, manganese ion, lead ion, barium ion, arsenic ion, selenium ion, antimony ion, iron ion, hexavalent It is selected from the group consisting of chromium ions and mercury ions, and the color change may appear differently depending on the type of the heavy metal ions.

The ion imprinted polymer is a copper ion imprinted polymer, zinc ion imprinted polymer, cobalt ion imprinted polymer, nickel ion imprinted polymer, silver ion imprinted polymer, cadmium ion imprinted polymer, trivalent chromium ion imprinted polymer, manganese ion imprinted polymer, lead ion imprinted It may be selected from the group consisting of polymers, barium ion polymers, arsenic ion polymers, selenium ion polymers, antimony ion polymers, iron ion polymers, hexavalent chromium ion polymers, and mercury ion imprinted polymers.

When the ion imprinted polymer is a zinc ion imprinted polymer, the zinc ion imprinted polymer may be formed by mixing a template ion, a monomer, a crosslinking agent, and an initiator in a molar ratio of 1: 3 or more: 13 or more: 0.04 or more.

The color developing solution is dithizone solution, diethyldithiocarbamate trihydrate (sodium diethyldithiocarbamate trihydrate, C ₅ H ₁₀ NNaS3H ₂ O), dimethylglyoxim (CH ₃ ) ₂ C ₂ (NOH) ₂ ), zincon (C ₂ 0H ₁₅ O6N ₄ SNa), o-phenanthroline dihydrogen chloride (1,10-phenanthroline dihydrogen chloride, C ₁₂ H ₈ N ₂ 2HCl), and diphenylcarbazide (1,5- diphenylcarbazide, C ₁₃ H ₁₄ N ₄ O) may be selected from the group consisting of.

The heavy metal ion detection sensor based on the ion-imprinted polymer according to the present invention for achieving the above object comprises an ion-imprinted polymer having a binding cavity for heavy metal ions, and an ion-imprinted polymer by adding a heavy metal ion sample to the heavy metal ion detection sensor The heavy metal ions are adsorbed to and the color developing solution is added to the ion-imprinted polymer adsorbed with the heavy metal ions to color the ion-imprinted polymer adsorbed with the heavy metal ions, and the heavy metal ions are detected by the color change of the colored ion-imprinted polymer. From the image of the ion-imprinted polymer, the RGB value of the colored ion-imprinted polymer may be measured, and the concentration of heavy metal ions may be calculated based on the measured RGB value.

In the calculation of the concentration of heavy metal ions, Euclidean distances (Eds) are calculated by correcting the measured RGB value as a reference RGB value, and the following equation is based on the calculated Euclidean distance.

y = 0.6997x + 15.2566

y: Euclidean distance

x: concentration of heavy metal ions (mg/L)

The concentration of heavy metal ions can be calculated using.

After the concentration of the heavy metal ions is calculated, the calculated concentration of the heavy metal ions may be displayed on the user terminal, or the calculated concentration of the heavy metal ions may be transmitted to an external terminal.

When the ion imprinted polymer is a zinc ion imprinted polymer, the zinc ion imprinted polymer may be formed by mixing a template ion, a monomer, a crosslinking agent, and an initiator in a molar ratio of 1: 3 or more: 13 or more: 0.04 or more.

According to the present invention, there is an advantage of being able to easily and easily detect heavy metal ions at a work site and measure the concentration of heavy metal ions without expensive detection equipment or pretreatment processes.

1 is a schematic flowchart of a method for detecting heavy metal ions based on an ion-imprinted polymer according to an embodiment of the present invention.

2 is a schematic flowchart of a method for detecting heavy metal ions based on an ion-imprinted polymer according to another embodiment of the present invention.

3 is a graph comparing the adsorption amount (q _e ) of zinc ions to the zinc ion imprinted polymer.

4 shows the color change of the zinc ion imprinted polymer according to the concentration of the zinc ion standard solution.

It should be noted that technical terms used in the present specification are only used to describe specific embodiments, and are not intended to limit the present invention. In addition, the technical terms used in the present specification should be interpreted as generally understood by those of ordinary skill in the technical field to which the present invention belongs, unless otherwise defined in the present specification, and excessively comprehensive It should not be construed as a person or an excessively reduced meaning In addition, when a technical term used in the present specification is an incorrect technical term that does not accurately express the spirit of the present invention, it should be replaced with a technical term that can be correctly understood by those skilled in the art.

In addition, general terms used in the present invention should be interpreted as defined in the dictionary or according to the context before and after, and should not be interpreted as an excessively reduced meaning.

In addition, the singular expression used in the present specification includes a plurality of expressions, unless the context clearly indicates otherwise. In the present application, terms such as "consist of" or "include" should not be construed as necessarily including all of the various elements or various steps described in the specification, and some of the elements or some steps It may not be included, or it should be interpreted that it may further include additional elements or steps.

Hereinafter, the present invention will be described in more detail through examples, but the scope of the present invention is not limited to the following examples.

1 is a schematic flowchart of a method for detecting heavy metal ions based on an ion-imprinted polymer according to an embodiment of the present invention.

Referring to Figure 1, the method for detecting heavy metal ions based on an ion-imprinted polymer according to an embodiment of the present invention includes an adsorption step (S10), a color development step (S20), a detection step (S30), a receiving step (S40), and a measuring step. It may include (S50) and a concentration calculation step (S60).

In the adsorption step (S10), a heavy metal ion sample may be added to a heavy metal ion detection sensor based on the ion-imprinted polymer to adsorb heavy metal ions to the ion-imprinted polymer.

In the heavy metal ion detection sensor based on the ion imprinting polymer, the ion imprinting polymer in the detection sensor is different depending on the heavy metal ion to be detected.

Heavy metal ions to be detected include copper ions, zinc ions, cobalt ions, nickel ions, silver ions, cadmium ions, trivalent chromium ions, manganese ions, lead ions, barium ions, arsenic ions, selenium ions, antimony ions, iron ions, 6 It may be selected from the group consisting of chromium ions and mercury ions.

The ion imprinting polymer in the heavy metal ion detection sensor is a copper ion imprinted polymer, zinc ion imprinted polymer, cobalt ion imprinted polymer, nickel ion imprinted polymer, silver ion imprinted polymer, cadmium ion imprinted polymer, trivalent chromium ion imprinted polymer, manganese ion imprinted polymer , Lead ion imprinted polymer, barium ion polymer, arsenic ion polymer, selenium ion polymer, antimony ion polymer, iron ion polymer, hexavalent chromium ion polymer, and mercury ion imprinted polymer may be selected from the group consisting of.

When the ion imprinting polymer in the heavy metal ion detection sensor is a zinc ion imprinting polymer, the zinc ion imprinting polymer may be formed by mixing a template ion, a monomer, a crosslinking agent, and an initiator in a molar ratio of 1: 3 or more: 13 or more: 0.04 or more. .

In the color development step (S20), a color developing solution may be added to the ion-imprinted polymer to which heavy metal ions are adsorbed, so that the ion-imprinted polymer to which heavy metal ions are adsorbed may be colored.

The color developing solution is dithizone solution, diethyldithiocarbamate trihydrate (sodium diethyldithiocarbamate trihydrate, C ₅ H ₁₀ NNaS3H ₂ O), dimethylglyoxim (CH ₃ ) ₂ C ₂ (NOH) ₂ ), zincon (C ₂ 0H ₁₅ O6N ₄ SNa), o-phenanthroline dihydrogen chloride (1,10-phenanthroline dihydrogen chloride, C ₁₂ H ₈ N ₂ 2HCl), and diphenylcarbazide (1,5- diphenylcarbazide, C ₁₃ H ₁₄ N ₄ O) may be selected from the group consisting of.

In the detection step S30, heavy metal ions may be detected by color change of the colored ion-imprinted polymer.

The color change of the colored ion-imprinted polymer may be different depending on the adsorbed heavy metal.

Copper ions (under methanol solvent) are pale yellow, zinc ions (under methanol solvent) pink, cobalt ions (under methanol solvent) red, nickel ions (under methanol solvent) red, and silver ions (tetrahydro In a furan solvent) yellow, cadmium ions (under methanol solvent) orange, trivalent chromium ions (under methanol solvent) dark blue, manganese ions (under methanol solvent) blue-green, lead ions (methanol solvent) Lower) is orange, barium ions (under methanol solvent) are dark blue, arsenic ions (under methanol solvent) are orange, selenium ions (under methanol solvent) are dark blue, and antimony ions (under methanol solvent) are dark blue. Blue, iron ions (in methanol solvent) light yellow, hexavalent chromium ions (in methanol solvent) dark blue, and mercury ions (in benzene and chloroform solvents) orange color.

As described above, according to the present invention, there is an advantage of being able to easily confirm the presence of heavy metal ions to be detected by color change of the colored ion-imprinted polymer.

In the receiving step (S40), an image of the colored ion imprinted polymer may be received.

In this case, the image is photographed and formed by various devices such as a user terminal, a heavy metal ion detection sensor, and a heavy metal ion meter including the sensor, and for this purpose, the device may be provided with a photographing unit.

However, the present invention is not necessarily limited thereto, and in another alternative embodiment, the heavy metal ion detection sensor or the heavy metal ion measuring device equipped with the sensor does not have a photographing unit, and is photographed by the photographing unit of the user terminal. The thus formed image may be received by the user terminal, the heavy metal ion detection sensor, or a heavy metal ion meter equipped with the sensor.

In addition, it is preferable that the photographing is performed under a certain brightness of lighting, and for this purpose, the device may be provided with a lighting unit capable of adjusting brightness, but the present invention is not limited thereto.

In the measuring step S50, the RGB value of the colored ion imprinted polymer may be measured from the received image. The RGB value of the colored ion-imprinted polymer may be measured through a predetermined program stored in the device or a predetermined application installed in the device.

In the concentration calculation step S60, the concentration of heavy metal ions may be calculated based on the measured RGB value.

To describe the concentration calculation step (S60) in more detail, the concentration calculation step (S60), calculating the Euclidean distances (Euclidean distances, Eds) by correcting the measured RGB value as a reference RGB value, and It may include calculating the concentration of heavy metal ions based on the Euclidean distance.

In the step of calculating the concentration of heavy metal ions based on the calculated Euclidean distance, the concentration of heavy metal ions (mg/L) may be calculated using the equation y = 0.6997x + 15.2566.

Here, y is the Euclidean distance, and x is the concentration of heavy metal ions.

2 is a schematic flowchart of a method for detecting heavy metal ions based on an ion-imprinted polymer according to another embodiment of the present invention.

Referring to Figure 2, the method for detecting heavy metal ions based on an ion-imprinted polymer according to another embodiment of the present invention includes an adsorption step (S10), a color development step (S20), a detection step (S30), a reception step (S40), and a measurement. It may include a step (S50), a concentration calculation step (S60), a display step (S70) and a transmission step (S80).

The adsorption step (S10), the color development step (S20), the detection step (S30), the receiving step (S40), the measuring step (S50) and the concentration calculating step (S60) are described with reference to FIG. Since it is the same as, its description will be omitted.

In the display step S70, the calculated concentration of heavy metal ions may be displayed on a predetermined device, such as the user terminal, the heavy metal ion detection sensor, or a heavy metal ion meter including the sensor. In addition, in addition to the calculated concentration of heavy metal ions, a measured RGB value may also be displayed.

In certain devices, such as the user terminal, the heavy metal ion detection sensor, or a heavy metal ion meter including the sensor, a display unit (not shown) may be provided to output the calculated concentration of heavy metal ions. The display unit forms a layered structure with the touch sensor or is integrally formed, thereby implementing a touch screen. Such a touch screen may function as a user input unit providing an input interface between the user terminal and the user, and may provide an output interface between the user terminal and the user.

In the transmitting step (S80), a predetermined device such as the user terminal, the heavy metal ion detection sensor, or a heavy metal ion meter including the sensor may transmit the calculated concentration of the heavy metal ion to an external terminal. More specifically, the predetermined device is equipped with a wireless communication unit (not shown), and the calculated concentration of heavy metal ions can be transmitted to an external terminal through the wireless communication unit.

The wireless communication unit may include one or more modules that enable wireless communication between a user terminal and a wireless communication system, between a user terminal and another terminal, or between a user terminal and a network in which an external server is located.

The external terminal may be a manager server that manages the concentration of heavy metal ions at each site, or may be a terminal of another user who detects heavy metal ions at different sites.

In this embodiment, the transmission step (S80) is performed after the display step (S70), but the present invention is not necessarily limited thereto, and only one of the display step (S70) and the transmission step (S80) is performed. May be.

The heavy metal ion detection sensor based on the ion imprinted polymer according to the present invention may include an ion imprinted polymer having a binding cavity for heavy metal ions.

At this time, the heavy metal ions are copper ions, zinc ions, cobalt ions, nickel ions, silver ions, cadmium ions, trivalent chromium ions, manganese ions, lead ions, barium ions, arsenic ions, selenium ions, antimony ions, and iron ions. , Hexavalent chromium ion, and mercury ion may be selected from the group consisting of.

At this time, the ion imprinted polymer is a copper ion imprinted polymer, a zinc ion imprinted polymer, a cobalt ion imprinted polymer, a nickel ion imprinted polymer, a silver ion imprinted polymer, a cadmium ion imprinted polymer, a trivalent chromium ion imprinted polymer, a manganese ion imprinted polymer, and lead. It may be selected from the group consisting of an ion imprinted polymer, a barium ion polymer, an arsenic ion polymer, a selenium ion polymer, an antimony ion polymer, an iron ion polymer, a hexavalent chromium ion polymer, and a mercury ion imprinted polymer.

When the ion imprinted polymer is a zinc ion imprinted polymer, the zinc ion imprinted polymer may be formed by mixing a template ion, a monomer, a crosslinking agent, and an initiator in a molar ratio of 1: 3 or more: 13 or more: 0.04 or more.

As the molar ratio of the monomer is lower than 3, the content of the monomer is relatively small, and thus, there is a problem in that the bonding cavities are less formed during the synthesis of the zinc ion imprinted polymer, and the adsorption selectivity for zinc ions is poor.

As the molar ratio of the crosslinking agent is lower than 13, the crosslinking between the template ion-monomer conjugate does not occur strongly when synthesizing a polymer material imprinted with zinc ions, so that the physical strength decreases, and thus there is a problem that a stable cavity cannot be formed.

When the molar ratio of the initiator is lower than 0.04, there is a problem in that the crosslinking between the template ion-monomer conjugate does not sufficiently occur when synthesizing a zinc ion-imprinted polymer material, and thus curing is not performed.

Hereinafter, preparation examples and examples will be described to aid in understanding the present invention. However, the following description only corresponds to an example of the contents and effects of the present invention, and the scope and effects of the present invention are not necessarily limited thereto.

[Production Example 1]

After dissolving 0.4 mmol of zinc nitrate in 1 mL of acetonitrile, 1.2 mmol of 4-vinylpyridine (hereinafter referred to as '4-VP'), and ethylene glycol dimethylacrylate (hereinafter referred to as'EGDMA') 6 mmol, 0.02 mmol of benzoyl peroxide (hereinafter'BPO') are sequentially added. After that, the mixture is purged with nitrogen (N ₂ ) for 20 seconds.

The nitrogen-purged mixture was placed in an oven at 110° C. and bulk polymerization was performed for 45 minutes. Thereby, a zinc ion imprinted polymer bulk is formed.

The resulting zinc ion imprinted polymer bulk was stirred with a removal solvent for 1 hour. At this time, the removal solvent includes acetic acid and methanol, and acetic acid and methanol are mixed in a volume ratio of 1:9. Thereafter, the zinc ion-imprinted polymer from which zinc ions have been removed and methanol are mixed and stirred for 12 hours. Through the removal step, the template ions were removed from the zinc ion-imprinted polymer bulk. The zinc ion imprinted polymer from which the template ion has been removed is dried at 65° C. for 12 hours.

[Production Example 2]

In the material mixing step, except that 5 mmol of EGDMA was used, the remaining conditions were the same as in Preparation Example 1.

[Production Example 3]

In the material mixing step, the remaining conditions were the same as in Preparation Example 1, except that 0.7 mmol of zinc nitrate, 2.4 mmol of 4-VP, 12 mmol of EGDMA, and 0.62 mmol of BPO were used.

[Production Example 4]

In the material mixing step, except that 0.7 mmol of zinc nitrate, 6.1 mmol of 4-VP, 12 mmol of EGDMA, and 0.66 mmol of BPO were used, the remaining conditions were the same as in Preparation Example 1.

[Production Example 5]

In the material mixing step, the remaining conditions were the same as in Preparation Example 1, except that 0.04 mmol of zinc nitrate, 0.04 mmol of 4-VP, 0.4 mmol of EGDMA, and 0.0016 mmol of BPO were used.

[Adsorption experiment]

Each experiment was performed by shaking 0.1 g of a zinc ion-imprinted polymer with 16 mL of a zinc standard solution for 12 hours. At the end of the experiment, the zinc ion imprinted polymer and the non-zinc ion imprinted polymer were removed from the zinc ion standard solution using a centrifuge, and the supernatant was filtered with a 0.2 μm pore size syringe filter.

3 is a graph comparing the adsorption amount of zinc ions to a zinc ion imprinted polymer.

3, the zinc ion polymer produced according to Preparation Example 1 showed the highest adsorption amount of zinc ions, and the zinc ion polymer produced according to Preparation Examples 2 to 4 showed a good level of adsorption of zinc ions. Showed. On the other hand, in the case of the zinc ion polymer produced according to Preparation Example 5, it was found that the adsorption amount of zinc ions was low, so that the adsorption property to zinc ions was poor.

When converted into a molar ratio of the template ion, monomer, crosslinking agent, and initiator used in Preparation Examples 1 to 5, they are shown in Table 1 below.

division	Template ion	Monomer	Crosslinking agent	Initiator
Manufacturing Example 1	One	3	15	0.04
Manufacturing Example 2	One	3	13	0.04
Manufacturing Example 3	One	4	17	0.90
Manufacturing Example 4	One	9	18	0.96
Manufacturing Example 5	One	One	10	0.04

The adsorption amount (q _e ) in FIG. 3 was calculated through the following equation.

[Example 1]

16 mL of a zinc ion standard solution having a concentration of 59 mg/L was added to 0.1 g of the zinc ion-imprinted polymer of Preparation Example 1, and adsorption was performed for 12 hours at 25°C in a shaking incubator. The zinc ion imprinted polymer and the zinc ion standard solution were separated using a centrifuge. The separated zinc ion imprinted polymer was dried in an oven at 60° C. for at least 5 hours.

After the dried zinc ion-imprinted polymer was cooled, 8 mL of a 1.0 mM dithizone solution was added and shaken in a shaking incubator for 3 hours. Zinc ion imprinted polymer and dithizone solution were separated using a centrifuge.

The zinc ion-imprinted polymer colored by the dithizone solution has a pale pink color.

The colored zinc ion imprinted polymer was photographed using a user terminal under constant lighting. The RGB value of the zinc ion imprinted polymer was measured from the captured image through a program or application installed in the user terminal. The measured RGB value may be output through the display unit of the user terminal.

The measured RGB values were corrected to the reference RGB values to calculate Euclidean distances (Eds). In this case, the reference RGB value refers to the RGB value of a zinc ion imprinted polymer adsorbing only methanol without zinc ions under the same conditions.

Euclidean distance (Eds)=

R _f , G _f , B _f = Zinc ion polymer R, G, B values adsorbed with zinc ion standard solution

R _i , G _i , B _i = Zinc ion polymer R, G, B values adsorbed with methanol

Through a program or application installed in the user terminal, the concentration of heavy metal ions was calculated based on the calculated Euclidean distance.

At this time, the applied calculation formula is as follows.

y = 0.6997x + 15.2566

y: Euclidean distance

x: concentration of heavy metal ions (mg/L)

[Example 2]

A standard solution of zinc ion with a concentration of 119 mg/L was used. The remaining conditions were carried out in the same manner as in Example 1.

[Example 3]

A standard solution of zinc ion at a concentration of 178 mg/L was used. The remaining conditions were carried out in the same manner as in Example 1.

[Example 4]

A standard solution of zinc ion at a concentration of 238 mg/L was used. The remaining conditions were carried out in the same manner as in Example 1.

4 shows the color change of the zinc ion imprinted polymer according to the concentration of the zinc ion standard solution.

Referring to FIG. 4, when a zinc ion standard solution having a concentration of 0 mg/L is used, there is almost no color change of the zinc ion imprinted polymer. On the other hand, the zinc ion-imprinted polymers of Examples 1, 2, 3, and 4 exhibited a pale pink color, and the darker the zinc ion concentration, the stronger the pale pink color development.

Table 2 below shows the Euclidean distance values according to the concentration of the zinc ion standard solution. The Euclidean distance was calculated over three times for the concentration of each zinc ion standard solution and averaged.

Zinc ion standard solution concentration (mg/L)	Euclidean distance (Eds)
	1 time	Episode 2	3rd time		Average
59	46	55	59	53 (±6)
119	98	109	99	102 (±5)
178	145	149	142	145 (±3)
238	178	178	175	177 (±1)

Based on the values in Table 2, y = 0.6997x + 15.2566 was derived.

y: Euclidean distance, x: concentration of heavy metal ions

In the above, a method for detecting zinc ions based on a zinc ion imprinting polymer and a sensor for detecting zinc ions have been described, but the present invention is not limited thereto.

In another alternative embodiment, copper ion imprinted polymer, cobalt ion imprinted polymer, nickel ion imprinted polymer, silver ion imprinted polymer, cadmium ion imprinted polymer, trivalent chromium ion imprinted polymer, manganese ion imprinted polymer, lead ion imprinted polymer, barium A heavy metal ion detection method and a heavy metal ion detection sensor based on an ionic polymer, an arsenic ion polymer, a selenium ion polymer, an antimony ion polymer, an iron ion polymer, a hexavalent chromium ion polymer, or a mercury ion imprinted polymer may be implemented.

In the above, specific preferred embodiments of the present invention have been described, but the present invention is not limited to the specific embodiments described above, and the technical field to which the present invention pertains without departing from the gist of the present invention claimed in the claims. Anyone of ordinary skill in the above can of course implement various modifications, as well as such modifications are within the scope of the claims.

Heavy metal ion detection method based on ion-imprinted polymer that adsorbs heavy metal ions on the ion-imprinted polymer in the heavy metal ion detection sensor, then develops color, detects heavy metal ions by color change of the colored ion-imprinted polymer, and measures the concentration of heavy metal ions And a detection sensor.

Claims (11)

1. Adsorbing heavy metal ions to the ion-imprinted polymer by adding a heavy metal ion sample to a heavy metal ion detection sensor based on the ion-imprinted polymer;

   Adding a color developing solution to the ion-imprinted polymer to which heavy metal ions are adsorbed to develop color of the ion-imprinted polymer to which heavy metal ions are adsorbed;

   Detecting heavy metal ions by color change of the colored ion-imprinted polymer;

   Receiving an image of the colored ion imprinted polymer;

   Measuring the RGB value of the colored ion-imprinted polymer from the received image; And

   Calculating the concentration of heavy metal ions based on the measured RGB value; A method for detecting heavy metal ions based on an ion-imprinted polymer, comprising: a.
2. The method of claim 1,

   The step of calculating the concentration of the heavy metal ions,

   Calculating Euclidean distances (Eds) by correcting the measured RGB value to a reference RGB value; And

   Based on the calculated Euclidean distance, the following equation

   y = 0.6997x + 15.2566

   y: Euclidean distance

   x: concentration of heavy metal ions (mg/L)

   Calculating the concentration of heavy metal ions by using; ion-imprinting polymer-based heavy metal ion detection method comprising a.
3. The method of claim 1,

   After the step of calculating the concentration of the heavy metal ions,

   Displaying the calculated concentration of heavy metal ions on the user terminal, or transmitting the calculated concentration of heavy metal ions to an external terminal; ion-imprinted polymer-based heavy metal ion detection method further comprising.
4. The method of claim 1,

   The heavy metal ion is copper ion, zinc ion, cobalt ion, nickel ion, silver ion, cadmium ion, trivalent chromium ion, manganese ion, lead ion, barium ion, arsenic ion, selenium ion, antimony ion, iron ion, hexavalent Selected from the group consisting of chromium ions and mercury ions,

   A method for detecting heavy metal ions based on an ion-imprinted polymer, characterized in that a color change in color appears differently depending on the type of the heavy metal ions.
5. The method of claim 1,

   The ion imprinted polymer is a copper ion imprinted polymer, zinc ion imprinted polymer, cobalt ion imprinted polymer, nickel ion imprinted polymer, silver ion imprinted polymer, cadmium ion imprinted polymer, trivalent chromium ion imprinted polymer, manganese ion imprinted polymer, lead ion imprinted Heavy metal ion detection based on ion-engraved polymer, characterized in that it is selected from the group consisting of polymer, barium ion polymer, arsenic ion polymer, selenium ion polymer, antimony ion polymer, iron ion polymer, hexavalent chromium ion polymer, and mercury ion imprinted polymer Way.
6. The method of claim 1,

   When the ion imprinted polymer is a zinc ion imprinted polymer,

   The zinc ion imprinted polymer,

   A method for detecting heavy metal ions based on an ion-imprinted polymer, characterized in that a template ion, a monomer, a crosslinking agent, and an initiator are mixed in a molar ratio of 1: 3 or more: 13 or more: 0.04 or more.
7. The method of claim 1,

   The color developing solution is dithizone solution, diethyldithiocarbamate trihydrate (sodium diethyldithiocarbamate trihydrate, C ₅ H ₁₀ NNaS3H ₂ O), dimethylglyoxim (CH ₃ ) ₂ C ₂ (NOH) ₂ ), zincon (C ₂ 0H ₁₅ O6N ₄ SNa), o-phenanthroline dihydrogen chloride (1,10-phenanthroline dihydrogen chloride, C ₁₂ H ₈ N ₂ 2HCl), and diphenylcarbazide (1,5- Diphenylcarbazide, C ₁₃ H ₁₄ N ₄ O) ion-imprinted polymer-based heavy metal ion detection method, characterized in that selected from the group consisting of.
8. As a heavy metal ion detection sensor based on ion imprinted polymer,

   It includes an ion-imprinted polymer having a binding cavity for heavy metal ions,

   A heavy metal ion sample is applied to the heavy metal ion detection sensor to adsorb heavy metal ions to the ion-imprinted polymer, and a color developing solution is added to the ion-imprinted polymer adsorbed with heavy metal ions to color the ion-imprinted polymer adsorbed with heavy metal ions. It detects heavy metal ions by color change of the imprinted polymer, measures the RGB value of the colored ionic imprinted polymer from the image of the colored ionic imprinted polymer, and calculates the concentration of heavy metal ions based on the measured RGB values. Heavy metal ion detection sensor based on ion imprinted polymer.
9. The method of claim 8,

   In the calculation of the concentration of heavy metal ions, Euclidean distances (Eds) are calculated by correcting the measured RGB value as a reference RGB value, and the following equation is based on the calculated Euclidean distance.

   y = 0.6997x + 15.2566

   y: Euclidean distance

   x: concentration of heavy metal ions (mg/L)

   Ion-engraved polymer-based heavy metal ion detection sensor, characterized in that to calculate the concentration of heavy metal ions using.
10. The method of claim 8,

    After calculation of the concentration of the heavy metal ions, the calculated concentration of the heavy metal ions is displayed on the user terminal, or the calculated concentration of the heavy metal ions is transmitted to an external terminal. sensor.
11. The method of claim 8,

    When the ion imprinted polymer is a zinc ion imprinted polymer,

    The zinc ion imprinted polymer,

    A heavy metal ion detection sensor based on an ion-imprinted polymer, characterized in that the template ion, the monomer, the crosslinking agent, and the initiator are mixed in a molar ratio of 1: 3 or more: 13 or more: 0.04 or more.

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