WO2020258883A9 - Biological scaffold and preparation method therefor and use thereof - Google Patents

Biological scaffold and preparation method therefor and use thereof Download PDF

Info

Publication number
WO2020258883A9
WO2020258883A9 PCT/CN2020/074195 CN2020074195W WO2020258883A9 WO 2020258883 A9 WO2020258883 A9 WO 2020258883A9 CN 2020074195 W CN2020074195 W CN 2020074195W WO 2020258883 A9 WO2020258883 A9 WO 2020258883A9
Authority
WO
WIPO (PCT)
Prior art keywords
biological
scaffold
biological matrix
matrix
preparation
Prior art date
Application number
PCT/CN2020/074195
Other languages
French (fr)
Chinese (zh)
Other versions
WO2020258883A1 (en
Inventor
马少华
赵浩然
蒋盛威
Original Assignee
清华-伯克利深圳学院筹备办公室
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 清华-伯克利深圳学院筹备办公室 filed Critical 清华-伯克利深圳学院筹备办公室
Publication of WO2020258883A1 publication Critical patent/WO2020258883A1/en
Publication of WO2020258883A9 publication Critical patent/WO2020258883A9/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
    • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
    • A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3808—Endothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/258—Genetic materials, DNA, RNA, genes, vectors, e.g. plasmids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A61L2300/414—Growth factors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Dermatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Transplantation (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Hospice & Palliative Care (AREA)
  • Dispersion Chemistry (AREA)
  • Endocrinology (AREA)
  • Cardiology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)

Abstract

A biological scaffold and a preparation method therefor and use thereof. The biological scaffold comprises a biological matrix and long chain DNA entangled in the biological matrix; the biological scaffold has a pore structure therein. The biological scaffold employs the biological matrix as the main scaffold material, long chain DNA is entangled in the biological matrix as a mechanical lock to lock a biological matrix fiber, the sol containing a large amount of monodisperse droplets is quickly gelated under the action of shear force, shortening the gelation time of the natural biological matrix, and increasing the gelation speed by 10 to 100 times, the pore structure promotes the exchange of substances in the biological matrix, providing oxygen and nutrients for the cells, discharging metabolic wastes, and improving the survival of the cell transplantation.
PCT/CN2020/074195 2019-06-27 2020-02-03 Biological scaffold and preparation method therefor and use thereof WO2020258883A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910569475.5 2019-06-27
CN201910569475.5A CN110180026B (en) 2019-06-27 2019-06-27 Biological scaffold and preparation method and application thereof

Publications (2)

Publication Number Publication Date
WO2020258883A1 WO2020258883A1 (en) 2020-12-30
WO2020258883A9 true WO2020258883A9 (en) 2021-02-04

Family

ID=67723822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2020/074195 WO2020258883A1 (en) 2019-06-27 2020-02-03 Biological scaffold and preparation method therefor and use thereof

Country Status (2)

Country Link
CN (1) CN110180026B (en)
WO (1) WO2020258883A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110180026B (en) * 2019-06-27 2021-02-09 清华-伯克利深圳学院筹备办公室 Biological scaffold and preparation method and application thereof
CN110920050A (en) * 2019-11-15 2020-03-27 清华-伯克利深圳学院筹备办公室 3D printing method, system and product
CN111197022A (en) * 2020-02-19 2020-05-26 清华大学深圳国际研究生院 Voxel printing biological ink and preparation method thereof
WO2022030856A1 (en) * 2020-08-06 2022-02-10 주식회사 강스템바이오텍 Method for producing highly functional artificial organs using aptamers
CN113083172B (en) * 2021-04-13 2022-04-19 清华大学 Nucleic acid hydrogel with improved mechanical properties and preparation method and application thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1237883C (en) * 2001-08-18 2006-01-25 阜新橡胶有限责任公司 Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof
CN102171234A (en) * 2008-08-05 2011-08-31 康奈尔大学 Photo-crosslinked nucleic acid hydrogels
US20150320833A1 (en) * 2012-12-13 2015-11-12 Steven L. Stice Ossification-inducing compositions and methods of use thereof
CN103910893B (en) * 2014-03-18 2016-02-24 清华大学 Poly-polypeptide-DNA hydrogel and preparation method thereof
CN103976941B (en) * 2014-05-09 2015-06-03 青岛大学 Hydrogel based on aptamer linking and preparation method and application thereof
EP3231419A1 (en) * 2016-04-14 2017-10-18 Universität Siegen Generation of dna hydrogels from linear building blocks
CN107596436A (en) * 2017-09-26 2018-01-19 天津大学 A kind of DNA fluorescence hydrogel and preparation method thereof
CN107556497B (en) * 2017-10-10 2019-03-19 淮阴师范学院 A kind of preparation method and application of hybridized hydrogel material
CN107778476B (en) * 2017-11-27 2020-04-10 淮阴师范学院 Construction method and application of supramolecular hydrogel material
CN110180026B (en) * 2019-06-27 2021-02-09 清华-伯克利深圳学院筹备办公室 Biological scaffold and preparation method and application thereof

Also Published As

Publication number Publication date
CN110180026B (en) 2021-02-09
WO2020258883A1 (en) 2020-12-30
CN110180026A (en) 2019-08-30

Similar Documents

Publication Publication Date Title
WO2020258883A9 (en) Biological scaffold and preparation method therefor and use thereof
CN101555455B (en) Culture/harvesting method of microalgae and system
NO135642B (en)
Randall et al. Self-folding immunoprotective cell encapsulation devices
Ahn et al. Functional cell-laden alginate scaffolds consisting of core/shell struts for tissue regeneration
CN102702559A (en) Ultra-multiple-pore hydrogel for microbial fermentation and preparation method and application thereof
CN103030420A (en) Method for preparing porous ceramics for absorbing and fixing myxobacteria
CN107441552B (en) Bioactive scaffold with bionic lotus root structure and preparation method and application thereof
CN114634388B (en) Controlled release fertilizer and preparation method thereof
Crawford Designer’s Guide to Lab Practice
Tong et al. Bio-coatings as immobilized microalgae cultivation enhancement: A review
Taidi et al. Colony development of laser printed eukaryotic (yeast and microalga) microorganisms in co-culture
CN102816695B (en) Micro-fluidic chip and method for studying effect of fluid shearing force on cell with the micro-fluidic chip
DE4411486C1 (en) Process and equipment for the cultivation and fermentation of microorganisms or cells in liquid media
CN212335051U (en) Organic fertilizer fermentation device for agricultural production
KR101797356B1 (en) Microalgae cultivating system comprising alginate films and illumunating fabrics and preparing method thereof
EP1473358A3 (en) Method and device for gassing and stirring materials
CN207727073U (en) A kind of microbial culture dish
Mignot et al. A new type of immobilized-cell photobioreactor with internal illumination by optical fibres
Liu et al. Plant cell immobilization in loofa sponge using two-way bubble circular system
Taub Why did carbon become the pseudo-limiting factor in aquatic closed ecological systems?
CN103305421A (en) Three-dimensional cell co-culture model based on PDMS (polydimethylsiloxane) and preparation method thereof
KR20240025813A (en) Artificial leaf structure
CN212786940U (en) Culture dish for tomato transgenic experiment
CN214300094U (en) Collecting device takes place in bacterial biofilm reaction

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20831977

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20831977

Country of ref document: EP

Kind code of ref document: A1

122 Ep: pct application non-entry in european phase

Ref document number: 20831977

Country of ref document: EP

Kind code of ref document: A1