CN1237883C - Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof - Google Patents
Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof Download PDFInfo
- Publication number
- CN1237883C CN1237883C CNB011280603A CN01128060A CN1237883C CN 1237883 C CN1237883 C CN 1237883C CN B011280603 A CNB011280603 A CN B011280603A CN 01128060 A CN01128060 A CN 01128060A CN 1237883 C CN1237883 C CN 1237883C
- Authority
- CN
- China
- Prior art keywords
- dna
- collagen
- compound
- concentration
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Materials For Medical Uses (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a collagen-DNA-Ag compound having the efficacy of bacteriostasis and a preparation method thereof, and the collagen-DNA-Ag compound belongs to biochemical products. The collagen-DNA-Ag compound having the efficacy of bacteriostasis is prepared from collagen having the concentration of 0.1 to 10%, DNA having the concentration of 0.1 to 5.0% and an AgNO3 solution having the concentration of 1 to saturation having the weight proportion of 1.5 to 2.5: 0.5 to 1.5: 0.05 to 0.2 by ion exchange, dialysis, degassing, forming, refrigeration, drying and sterilization. The collagen-DNA-Ag compound having the efficacy of bacteriostasis inhibits staphylococcus aureus, pseudomonas aeruginosa and colibacillus has the characteristics of promoting the formation of granulation tissues and inhibiting the formation of crusta on treating burns, scalds and hemostasis, and is used as biological coating material to be used in medical clinics. The collagen-DNA-Ag compound having the efficacy of bacteriostasis has the advantages of good abutting properties of wound surface, ventilation, water absorption, hemostasis, inflammation elimination and skin regeneration promotion.
Description
Technical field
The invention belongs to biochemical product, particularly a kind of collagen-DNA-Ag compound and preparation method thereof with bacteriostasis efficacy.
Background technology
At present, be used for existing a lot of researchs of wound repair and report about collagen film as a kind of clinical medicine biological dressing.The collagen that extracts from animal tissue can be made film by several different methods, as (making of artificial dermis-collagen+6-chondroitin sulfate film and partly performance measurement, Li Xiaojian etc. such as the film forming of saltouing, ammonia cure film forming and freeze drying film forming.Biomedical engineering magazine 1999; 16 (2); 151-153).In order to strengthen film strength, people have adopted glutaraldehyde cross-linking, artificial or native fiber double-deck bonding or impregnating technology (experimental study that several collagen type wound dressings are made, Wu Zhigu etc. again.Biomedical engineering magazine 1999; 16 (2); 147-150).Yet, though a kind of extraordinary Wound dressing that collagen film is up to now to be found, bacterial infection problem that very serious problem is exactly the surface of a wound in clinical practice.
The solution treatment burn back remnants that have report to be mixed and made into III Collagen Type VI and Ag-SD (flamazine) hinder face, and (III Collagen Type VI Ag-SD liquid treatment burn back remnants hinder face, Wu Xiaolong etc.Jiangsu medicine 1992; 18 (3); 137-138).It is to be that the III Collagen Type VI solution of 0.5-0.1% and the Ag-SD frost of half amount mix with concentration, is coated on the surface of a wound then and uses.But because collagen solution easy corrupt sex change in storage process, so this solution can only matching while using.And in this solution, tropocollagen molecule and Ag-SD molecule are mutually independently.
Te Kaiping 6-304241 has introduced a kind of argentiferous collagen sponge membrane.Its manufacture method is that water soluble silver salt is adsorbed onto the ceramic-like material or by after the ion exchange combination, makes the ceramic-like material that combines silver in the above sintering of silver point, pulverizing.Then this material is added in the collagen solution and stir.Freeze drying.Promptly get a kind of antibiotic property collagem membrane.In the collagen sponge membrane that this method makes, the absorption of ceramic silver-colored class material and collagen is physical adsorption.The antibacterial effect of gained collagem membrane depends on the granularity of ceramic silver-colored class material and the quantity that combines with collagen absorption.In conjunction with lack, influence antibacterial effect; In conjunction with manyly make the collagem membrane hardening that obtains again.
Cu
2+, Hg
2+, Ag
+Have sterilizing function etc. heavy metal ion, its common ground is by combining with enzyme that bacterial body includes the sulphydryl activity group it to be lost activity, thereby the bacterial growth breeding is suppressed.Equally, as heavy metal ion, it also can make protein lose biologically active.
Summary of the invention
The objective of the invention is to overcome above technical deficiency, a kind of collagen-DNA-Ag compound with bacteriostasis efficacy and preparation method thereof is provided, make that these metal ions and collagen are compound, that had both brought into play these metal ions presses down the mattress characteristic, the biologically active that keeps collagen again, produce a kind of collagen-based composite of excellent performance, be used as the clinical medicine biological dressing.
The present invention is by adopting DNA and Ag
+In conjunction with, and then compound with collagen, obtain a kind of collagen-DNA-Ag compound, solved the problem of collagen chance heavy metal ion inactivation sex change, prepare a kind of bacteria resistance function that has, have the collagen-DNA-Ag compound of certain intensity simultaneously concurrently.DNA can be combined into gel-like substance with tropocollagen molecule.When in the aqueous solution of DNA sodium salt, adding the silver soluble salting liquid,, make part Na in the DNA sodium salt by ion exchange
+The ore deposit is by Ag
+Replace, then, remove small ion free in the solution by dialysis again, can obtain containing Ag in a kind of molecule
+Dna solution.
The present invention also provides a kind of medicine that is used for the treatment of burn and hemostasis, and this medicine contains above-mentioned compound as active component.Described medicine can contain the assistant agent that contains usually in the similar medicine, forming agent etc., and can be made into various formulations, as dressing, tablet, pulvis etc.
The invention provides collagen-DNA--Ag compound and preparation method thereof:
Raw material of the present invention is:
The collagen aqueous solution adopts the collagen that extracts, concentration 0.1-10%, preferably 0.4-1.0% from calf-skin.That the DNA employing is extracted from milt, the DNA of molecular weight more than 1,000,000, concentration 0.1-5.0%, preferably 0.1-1.0%.Soluble silver salt uses is 1% to the full AgNO of concentration that closes
3The aqueous solution, preferably 1%-10%.
DNA is dissolved in the deionized water below 4 ℃ the aqueous dna of preparation concentration 0.1-5.0%.Add the 1% 1 full silver soluble saline solution that closes, stir 5-20min, dialysis 24-96h can not detect corresponding dissociated ion to extracellular fluid dialysis.
The collagen solution that with concentration is 0.1-10% is in-20-40 ℃ dialysis.Under 3.3-26.6KPa, decompression degassing 5-20min is under 0.05-0.5MPa, with (φ 0.05-1.0mm nozzle sprays in the above-mentioned dna solution.With the gel drop that the forms part of anhydrating, washing in 10 * 10 * 0.5cm model of packing into, is freezed under-20--200 ℃, with P
2O
5And KOH is desiccant, and vacuum drying or vacuum cooling drying get collagen sponge membrane under-30-40 ℃, 0.038-7.4KPa.Or the collagen solution that will spray into DNA stirs, a water white transparency shape latex, with its heated dry air atomized drying, promptly get collagen-DNA-Ag white powder with 0.3-1.0MPa, 80-200 ℃.Packing, sterilization.
Compared with prior art, the technology of the present invention effect:
1, provide a kind of collagen-DNA-Ag composite article to do biological dressing with bacteriostasis property, these goods have stronger inhibitory action to Staphylococcus aureus (staphylococcus aureus), pseudomonas aeruginosa (Pseudomonasaeruginosa), Escherichia coli (Escherichia coli), can suppress bacterial infection effectively when doing biological dressing.
2, in this collagen-DNA-Ag composite article with bacteriostasis property provided by the invention, Ag
+Combine and be hidden in big intramolecule with ionic bond with DNA, so have slow release.
3, this collagen-DNA-Ag composite article with bacteriostasis property provided by the invention is compared with liquid collagen goods, can at room temperature effectively preserve for a long time.
4, this collagen-DNA-Ag compound with bacteriostasis property provided by the invention according to the difference of drying means, can be made membranoid substance, powder use.
5, in according to the collagen-DNA-Ag sponge membrane product with bacteriostasis property provided by the invention, because DNA-Ag combines by Van der Waals force and hydrogen bond with tropocollagen molecule with molecular state, so, compare with the silver-colored collagem membrane of pottery, collagen-DNA-Ag sponge film softness is better, does not harden.
6, in collagen of the present invention-DNA-Ag compound, the polysaccharide macro-molecular DNA of introducing combines by Van der Waals force and hydrogen bond with tropocollagen molecule, is wound in network structure mutually, thereby, strengthened the mechanical strength of this collagen-based composite.
7, collagen that adopts among the present invention and Ag
+Compound media DNA is the same with collagen, all is the material from the biologically active of organism.Therefore, the biologically active that has guaranteed the collagen in collagen-DNA-Ag compound is not lost.
8, in this collagen-DNA-Ag composite article with bacteriostasis property provided by the invention, the pH value that Ag content can be by adjusting dna solution and the soluble silver salt amount of adding be regulated.
9, this collagen-based composite provided by the invention also can be attached to using on other base material.As various native fiber, staple fibre, macromolecular material etc.
Has collagen-DNA-Ag compound of bacteriostasis efficacy and preparation method thereof.
Collagen-DNA-Ag compound with bacteriostasis efficacy is by following feedstock production: collagen concentration is 0.1-10%, 0.4-1.0% preferably, DNA concentration 0.1-5.0%, preferably 0.1-1.0%, DNA is the DNA sodium salt, and the silver soluble salinity is 1% to the full AgNO that closes
3, 1%-10% preferably.Collagen: DNA: the Ag ratio is 1.5-2.5: 0.5-1.5: 0.05-0.2 (W/W/W).
Preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy, with concentration 0.1-10% collagen, concentration 0.1-5.0%DNA, concentration 1% to the full soluble silver salt of concentration that closes, through ion exchange, dialysis, the degassing, moulding, freezing, dry, sterilize and make medical antibacterial collagen-based composite.Ion exchange is dissolved in DNA in the deionized water that is chilled to 4 ℃ in advance, adds the full AgNO that closes of 1%-
3Solution stirs 5-20min, and reaction temperature-20-40 ℃, the DNA-AgNO after ion-exchange reactions finished
3The mixed liquor dialysis, temperature-20-40 ℃, time 24-96h.With the degassing of the collagen solution after the dialysis, pressure is 3.3-26.6KPa, time 5-20min.Collagen solution after the degassing is sprayed in the DNA-Ag solution nozzle bore 0.05-1.0mm, hole density 5-50/cm
2, pressure 0.05-0.5MPa.Collagen after the moulding-DNA-Ag compound is freezing, temperature-20--200 ℃.With the collagen after freezing-DNA-Ag compound drying, baking temperature-30-40 ℃, pressure 0.038-7.4KPa, desiccant are P
2O
5, KOH, liquid nitrogen, dry ice, be vacuum drying or vacuum cooling drying, collagen-DNA-Ag sponge film, packing, sterilization.Or the collagen solution that will spray into DNA stirs, a water white transparency shape latex, with the collagen-DNA-Ag latex atomized drying that obtains, dry is 0.3-1.0MPa with hot-blast pressure, temperature is 80-200 ℃, powdery collagen-DNA-Ag compound, packing is sterilized.Collagen-DNA-Ag compound has bacteriostasis efficacy, and the bacterial classification of inhibition has staphylococcus aureus, pseudomonas aeruginosa, Escherichia coli, is used for the treatment of the medicine of burn and hemostasis.
Description of drawings
Fig. 1, staphylococcus aureus---collagen-DNA-Ag sponge film.
Fig. 2, staphylococcus aureus---collagen-DNA sponge film.
Fig. 3, staphylococcus aureus---pure collagen sponge membrane.
Fig. 4, proof gold staphylococcus aureus.
Fig. 5, Pseudomonas aeruginosa---collagen-DNA-Ag sponge film.
Fig. 6, Pseudomonas aeruginosa---collagen-DNA sponge film.
Fig. 7, Pseudomonas aeruginosa---pure collagen sponge membrane.
Fig. 8, pure Pseudomonas aeruginosa.
Fig. 9, Escherichia coli---collagen-DNA-Ag sponge film.
Figure 10, Escherichia coli---collagen-DNA sponge film.
Figure 11, Escherichia coli---pure collagen sponge membrane.
Figure 12, pure Escherichia coli.
Embodiment
Embodiment 1
1g DNA under agitation is dissolved in the 1000ml deionized water that is chilled to 4 ℃ in advance.Add 1%AgNO
32g.Stirred 10 minutes.This solution is placed bag filter, dialysis 24-96h.
Get 0.7% collagen solution 300ml, under 4 ℃, dialysis 24--96h.The degassing is 10 minutes under 13.3KPa.Use diameter 1cm then, aperture 0.4mm, hole count is 50/cm
2Shower nozzle, under 0.15MPa pressure, it is sprayed in the above-mentioned dna solution.The gel that obtains is placed on the 100 purpose screen clothes drop part of anhydrating, be chilled to 4 ℃ deionized water washing three times in advance with 200ml, in the model of 10 * 10 * 0.5cm that packs into, cryogenic temperature-20 ℃ is used P
2O
5, KOH makees desiccant, vacuum drying can get 10 collagen protein sponge films, gross weight 3.1g, Ag content 0.21% (w/w).Packing, CO
60Irradiation.
Embodiment 2
5g DNA under agitation is dissolved in the 100ml deionized water that is chilled to 4 ℃ in advance, and transferring the solution pH value is 8-10, adds saturated AgNO
30.5g, stir 20min, dialysis 24-96h.
Get 0.5% collagen solution 2000ml, under 4 ℃, dialysis 24--96h, 10min outgases under 13.3KPa.Use diameter 1cm, aperture 0.5mm, 50/cm of hole count then
2Shower nozzle, under 0.1MPa pressure, it is sprayed in the above-mentioned dna solution.The gel that obtains is placed on the 100 purpose screen clothes drop part of anhydrating, be chilled to 4 ℃ deionized water washing three times in advance with 2000ml, in the model of 10 * 10 * 0.5cm that packs into, cryogenic temperature-20 ℃.Use P
2O
5, KOH makees desiccant, drying, 55 collagen protein sponge films, heavy 16.2g, Ag content 0.25% (w/w), packing, CO
60Irradiation.
Embodiment 3
1g DNA under agitation is dissolved in the 100ml deionized water that is chilled to 4 ℃ in advance, adds 2%AgNO
3Solution 1g stirs 10min, dialysis 24-96h.
Get 0.5% collagen solution 400ml, under 4 ℃, dialysis 24---96h, the 10min that outgases under 13.3KPa uses diameter 1cm, aperture 0.5mm, 50/cm of hole count then
2Shower nozzle, under 0.1MPa pressure, it is sprayed in the above-mentioned dna solution, stirring, is that 0.5MPa, temperature are 120 ℃ air-atomizing drying with the water white transparency latex pressure that obtains, and gets collagen-DNA-Ag composite powder 1.8g, Ag content 0.20% (w/w), packing, CO
60Irradiation.
Bacteriostatic experiment
Embodiment 4
(1), mixes the dull and stereotyped preparation of bacterium culture medium
The slant culture of cultured staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa is scraped a little lawn with oese respectively, make the 5ml bacteria suspension with sterilized nutrient broth medium then.It is standby with 10 times of nutrient broth dilutions to get this bacteria suspension 0.1ml.
Get 12 of sterile petri dish, grouping, numbering.Wherein 1-4 number each merit mouth of I group gone into the above-mentioned staphylococcus aureus bacteria suspension 1ml that has prepared; The II group respectively adds the above-mentioned Pseudomonas aeruginosa bacteria suspension 1ml that has prepared 5-8 number; The III group respectively adds the above-mentioned Escherichia coli bacteria suspension 1ml that has prepared 9-12 number.Add the 20ml nutrient broth agar medium that is cooled to about 50 ℃ more respectively, shake up, fully standby after the condensation.
(2), bacteriostatic test
With diameter 2.5cm, through CO
60Collagen-DNA-Ag after the sterilization, collagen-DNA composite sponge film and pure collagen sponge membrane are by after the following grouping, be affixed on and mix the dull and stereotyped middle position of bacterium liquid culture medium, carry out mark, be upside down in constant incubator in 35-37 ℃ and cultivated 24-48 hour, observe the bacterial growth situation.The grouping situation is as follows:
I group 1-4 number, the staphylococcus aureus experimental group:
1, collagen-DNA-Ag sponge film Fig. 1;
2, collagen-DNA sponge film Fig. 2;
3, collagen sponge membrane Fig. 3;
4, blank staphylococcus aureus Fig. 4.
II group 5-8 number, the Pseudomonas aeruginosa experimental group:
5, collagen-DNA-Ag sponge film Fig. 5;
6, collagen-DNA sponge film Fig. 6;
7, collagen sponge membrane Fig. 7;
8, blank Pseudomonas aeruginosa Fig. 8.
III group 9-12 number, the coli test group:
9, collagen-DNA-Ag sponge film Fig. 9;
10, collagen-DNA sponge film Figure 10;
11, collagen sponge membrane Figure 11;
12, blank Escherichia coli Figure 12.
(3), result of the test
Collagen-DNA-Ag sponge film all has bacteriostasis to three kinds of bacterium, and is more than the antibacterial circle diameter 3cm, clear; Collagen-DNA sponge film has inhibitory action to staphylococcus aureus, and is more than 24 hours antibacterial circle diameter 3cm, clear; 48 hours fuzzy.All the other two kinds of bacterium there is not bacteriostasis; Pure collagen sponge membrane does not all have bacteriostasis to three kinds of bacterium.
The hemostasis experiment
Embodiment 5
(1) laboratory animal and material
The product that collagen-DNA-Ag sponge film is provided by embodiment one and embodiment two; Gelfoam is produced by Nanjing the 3rd pharmaceutical factory; The laboratory animal large ear rabbit is provided by affiliated hospital of Dalian Medical College Animal Lab..
(2) foundation of animal model
Select 10 health large ear rabbits, body weight 2.5-3.0Kg, male and female are not limit, and single cage is raised, and the feed of freely drinking water experimentizes after 1 week at laboratory rearing.Per 5 one components become two groups of experimental group and control groups, make the hemorrhage surface of a wound of 2cm * 1cm size in large ear rabbit ear.Experimental group collagen-DNA-Ag sponge film, control group gelfoam film.
(3) observation index
After collagen-DNA-Ag sponge film and the deposited pressure of gelfoam film, record large ear rabbit ear surface of a wound bleeding stopping period.
(4) experimental result
Bleeding stopping period: collagen-DNA-Ag sponge film and gelfoam film bleeding stopping period are respectively 57.2 ± 15.4S and 96.0 ± 15.1S; Amount of bleeding is respectively 1.6 ± 0.3ml and 2.6 ± 0.7ml, and the two relatively has significant difference (P≤0.05).
Apply to press back 10min, 24h, 48h to observe that equal end sees that sponge comes off and bleeding again with collagen-DNA-Ag sponge film, seal the hemorrhage surface of a wound effectively.And gelfoam film three examples have the phenomenon of oozing out between dressing and tissue, and in addition, to the hemorrhage rapid rabbit ear portion surface of a wound, the foam structure face that gelatin passes in blood flow regular meeting oozes out.
According to above-mentioned zoopery, collagen-DNA-Ag sponge film haemostatic effect is better than gelfoam.
Collagen-DNA-Ag sponge film is to the treatment of burn
Embodiment 6
(1), case is selected
Remnants hindered the face patient after 4 examples were burnt, average dark II degree, III degree burn, and treatment group collagen-DNA-Ag sponge film 2 examples, control group collagen sponge 2 examples, surface of a wound bacterial culture is respectively staphylococcus aureus, and pseudomonas aeruginosa is a case each.
(2), method of disposal
The surface of a wound is done the wound tissue bacterial culture, counting before cleaning with stroke-physiological saline solution.The surface of a wound covers collagen-DNA-Ag sponge film, collagen sponge membrane respectively after cleaning with stroke-physiological saline solution, and the next day cleans with stroke-physiological saline solution and to change dressings once, relatively the result of the two treatment.
(3), treatment results
| Surface of a wound area (cm.) | The recovery from illness fate | The every gram bacterial population of the surface of a wound | ||
| Before the surface of a wound cleans | Behind the wound healing | |||
| The treatment group | 8.93 | 10 | 6.04 * 10 4 | ＜10 |
| 7.5 | 8 | 7.9 * 10 4 | ＜10 | |
| Control group | 9.92 | 11 | 9.5 * 10 5 | 5.1 * 10 2 |
| 8.51 | 10 | 7.85 * 10 5 | 6.87 * 10 2 | |
The result shows that the treatment group hinders the face cure time than control group remnants shortening is arranged slightly, and surface of a wound bacterial number differs greatly.Histopathology is observed and is found that 4 examples all have the granulation tissue characteristics, does not all have crust and forms.
Has the collagen of bacteriostasis efficacy-DNA-Ag compound, be used for the treatment of burn and hemostasis the characteristics that promote granulation tissue to form, suppress crust formation are arranged, being used for biological dressing uses at clinical medicine, soft comfortable, surface of a wound stickiness is good, ventilative, suction, hemostasis, inhibiting bacteria and diminishing inflammation promote skin regeneration.
Claims (14)
1, the collagen-DNA-Ag compound that has bacteriostasis efficacy is characterized in that by following feedstock production:
Collagen concentration is 0.1-10%, and DNA concentration is 0.1-5.0%, soluble silver salt AgNO
3Solution concentration is 1% to close collagen to full: DNA: the Ag ratio is 1.5-2.5: 0.5-1.5: 0.05-0.2 (W/W/W).
2, the collagen-DNA-Ag compound with bacteriostasis efficacy according to claim 1 is characterized in that DNA is the DNA sodium salt.
3, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 1 is characterized in that being is that 0.1-10%, DNA concentration are 0.1-5.0%, soluble silver salt AgNO with the collagen concentration
3Solution concentration is 1% to close concentration to full, and through ion exchange, dialysis, the degassing, the collagen solution that sprays into DNA stirs, water white transparency shape latex, moulding, freezing, dry, sterilize and make medical antibacterial sponge film collagen-DNA-Ag compound.
4, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 1 is characterized in that being is that 0.1-10%, DNA concentration are 0.1-5.0%, soluble silver salt AgNO with the collagen concentration
3Solution concentration is 1% to close concentration to full, and the collagen solution that sprays into DNA stirs, water white transparency shape latex, atomized drying, medical antibacterial powdery collagen-DNA-Ag compound is made in sterilization.
5, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3 is characterized in that in ion-exchange step, DNA is dissolved in the deionized water that is chilled to 4 ℃ in advance, adds the full AgNO that closes of 1%-
3Solution stirred 5-20 minute, and reaction temperature is-20-40 ℃.
6, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3, the DNA-AgNO after it is characterized in that ion-exchange reactions finished
3Mixed liquor dialysis, temperature be-20-40 ℃, and the time is 24-96h.
7, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3 is characterized in that in the dialysis step, with the dialysis of the collagen solution of 0.1-10%, the dialysis temperature is-and 20-40 ℃, the time is 24-96 hour.
8, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3 is characterized in that pressure is 3.3-26.6KPa with the degassing of the collagen solution after the dialysis, and the time is 5-20min.
9, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3 is characterized in that the collagen solution after the degassing is sprayed in the DNA-Ag solution, and nozzle bore 0.05-1.0mm, hole density are 5-50/cm
2, pressure is 0.05-0.5Mpa.
10, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3 is characterized in that the collagen after the moulding-DNA-Ag compound freezingly, temperature is-and 20--200 ℃.
11, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 3 is characterized in that the collagen after freezing-DNA-Ag compound drying, baking temperature is-and 30-40 ℃, pressure is 0.038-7.4KPa, desiccant is P
2O
5, KOH, liquid nitrogen, dry ice, drying means is vacuum drying or vacuum cooling drying, sponge film collagen-DNA-Ag compound.
12, according to claim 3 or 4 described preparation methods with collagen-DNA-Ag compound of bacteriostasis efficacy, it is characterized in that sterilization, sterilizing methods is Co
60Irradiation.
13, the preparation method with collagen-DNA-Ag compound of bacteriostasis efficacy according to claim 4, it is characterized in that the collagen-DNA-Ag latex atomized drying that will obtain, dry is 0.3-1.0MPa with hot-blast pressure, and temperature is 80-200 ℃, gets powdery collagen-DNA-Ag compound.
14, a kind of medicine that is used for the treatment of burn and hemostasis is characterized in that the compound that this medicine contains claim 1,2 described compounds or prepares according to each described method of claim 3 to 13.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011280603A CN1237883C (en) | 2001-08-18 | 2001-08-18 | Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011280603A CN1237883C (en) | 2001-08-18 | 2001-08-18 | Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1406489A CN1406489A (en) | 2003-04-02 |
| CN1237883C true CN1237883C (en) | 2006-01-25 |
Family
ID=4667957
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011280603A Expired - Fee Related CN1237883C (en) | 2001-08-18 | 2001-08-18 | Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1237883C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101161728B (en) * | 2007-09-21 | 2010-11-17 | 东华大学 | Gelatin-Ag nano compound having bacteriostasis efficacy and preparation method thereof |
| CN102408467B (en) * | 2010-09-26 | 2014-03-05 | 海口维瑅瑷生物研究院 | Method for drying protein in vacuum, protein product prepared and kit |
| CN108411621A (en) * | 2018-03-16 | 2018-08-17 | 三元控股集团有限公司 | A kind of preparation method of multifunctional fabric finishing agent |
| CN110180026B (en) * | 2019-06-27 | 2021-02-09 | 清华-伯克利深圳学院筹备办公室 | Biological scaffold and preparation method and application thereof |
| CN111214694B (en) * | 2020-03-31 | 2022-04-22 | 山东大鱼生物技术有限公司 | Dressing with functions of stopping bleeding and accelerating wound healing and preparation method thereof |
-
2001
- 2001-08-18 CN CNB011280603A patent/CN1237883C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1406489A (en) | 2003-04-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2019091150A1 (en) | Alginate wound repair dressing and preparation method thereof | |
| CN110354295B (en) | Photo-thermal conversion material and preparation method thereof | |
| CN101721691B (en) | Preparation for treating and restoring infective wound surface and preparation method thereof | |
| CN105778126A (en) | Genipin cross-linked biogel as well as preparation method and application thereof | |
| CN1325291A (en) | Anti-inflammatory and antimicrobial uses for bioactive glass compositions | |
| CN105797193B (en) | A kind of Thermo-sensitive arginine base long acting antibiotic aerogel dressing and preparation method thereof | |
| CN101255274A (en) | Composite nano silver-polyurethane antibiotic material and preparation thereof | |
| CN1395491A (en) | Polymer blends as biodegradable matrices for preparing biocomposites | |
| CN107185031B (en) | A kind of biologically active medical dressing and preparation method thereof | |
| CN103356692A (en) | Composite antibacterial gel and preparation method thereof | |
| Xiong et al. | Derma-like antibacterial polysaccharide gel dressings for wound care | |
| CN1775302A (en) | Chitose-gelatine sponge wound dressing preparing method | |
| CN1237883C (en) | Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof | |
| CN104784739A (en) | Preparation method of composite antibacterial dressing loaded with silver sulfadiazine | |
| CN104784103B (en) | Based on oligomeric amino acid amphiphile, amphiphilic molecule injectable anti-bacterial hydrogel | |
| CN1799639A (en) | Inflammation-eliminating, pain-stopping, bacteria-resisting, pain-relieving soluble hemostatic gauze and its processing method | |
| CN100560057C (en) | Wound-protection liquid and preparation method thereof | |
| CN111632188A (en) | Seaweed polysaccharide composite biological matrix dressing and preparation method thereof | |
| CN1723778A (en) | Silver-contg. type antibiotic agent, prepn. method and application thereof | |
| CN108434507B (en) | Ion-mediated dressing and preparation method and application thereof | |
| CN110507851A (en) | A kind of absorbable bone wax and preparation method thereof | |
| CN111298185B (en) | Camellia oil and tea seed meal antibacterial polypeptide medical dressing and preparation method and application thereof | |
| CN103893811B (en) | The application of a kind of preparation method of bio-vitric felt and products thereof | |
| Lagat | Biological and Chemical Extraction of Chitin and Chitosan from the Black Soldier Fly (Hermetia illucens) Exoskeleton and Antimicrobial Activity Against Selected Human Pathogenic Microbes | |
| CN107595819A (en) | A kind of Nano diamond modified liquid adhesive bandage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: FUXIN HUANYU RUBBER (GROUP) CO., LTD. Free format text: FORMER OWNER: FUXIN RUBBER CO., LTD. Effective date: 20111118 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 123000 FUXIN, LIAONING PROVINCE TO: ECONOMIC AND TECHNOLOGICAL DEVELOPMENT ZONE, LIAONING PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20111118 Address after: No. 93, C Road, 6 North Road, Liaoning economic and Technological Development Zone Patentee after: Fuxin Huanyu Rubber (Group) Co., Ltd. Address before: 123000, No. 27, electrician Road, Bayi Road, Haizhou District, Fuxin, Liaoning, Liaoning, Fuxin Patentee before: Fuxin Rubber Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060125 Termination date: 20160818 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |