WO2020235038A1 - Optical measuring device - Google Patents

Optical measuring device Download PDF

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Publication number
WO2020235038A1
WO2020235038A1 PCT/JP2019/020247 JP2019020247W WO2020235038A1 WO 2020235038 A1 WO2020235038 A1 WO 2020235038A1 JP 2019020247 W JP2019020247 W JP 2019020247W WO 2020235038 A1 WO2020235038 A1 WO 2020235038A1
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Prior art keywords
temperature
measurement
housing
measuring device
optical measuring
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PCT/JP2019/020247
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French (fr)
Japanese (ja)
Inventor
戸井田　秀基
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株式会社シーズテック
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Priority to JP2019559867A priority Critical patent/JP6710403B1/en
Priority to PCT/JP2019/020247 priority patent/WO2020235038A1/en
Publication of WO2020235038A1 publication Critical patent/WO2020235038A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase

Definitions

  • the present invention relates to an optical measuring device for measuring the concentration of dioxins or PCB (polychlorinated biphenyl) by a fluorescence detection method, for example.
  • Patent Document 1 As the optical measuring device as described above, there is one disclosed in Patent Document 1 below.
  • This device forms a complex by an antigen-antibody reaction between a sample containing dioxin, which is an antigen, and a fluorescently labeled antibody solution, and is not yet in the reaction solution in a measurement cell filled with an antigen derivative immobilization carrier. The reaction antibody is captured and the fluorescence intensity is measured.
  • the measurement unit of the device is equipped with the above-mentioned measurement cell, a pump for flowing the measurement sample solution through the flow path of the measurement cell, an excitation light source, an optical sensor, and a controller.
  • a metering pump such as a tube pump is used.
  • the sample solution for measurement there is a part in the flow path through which waste liquid, buffer solution for measurement, regeneration liquid, and sanitary liquid flow, and part of the flow path is the flow path of each liquid.
  • a flow path switching valve for switching between the two is provided.
  • the pump, many parts of the flow path, the flow path switching valve, etc. are provided inside the housing. Further, the flow path is formed of a resin tube.
  • Such a device is used in a certain temperature environment because the measurement result varies depending on the temperature of the measurement environment.
  • the flow path through which the measurement sample solution flows is formed of a resin tube, so as long as the measurement environment is within a predetermined temperature range, the measurement results are unlikely to vary.
  • the flow path is a resin tube
  • contamination such as erosion by the antigen or residue of the antigen.
  • the main object of the present invention is to improve the measurement accuracy in terms of temperature and cleanliness of the flow path.
  • a flow path for sending the measurement sample solution is provided inside the housing, and the measurement sample solution is mixed with a sample having an unknown concentration of antigen and a fluorescently labeled antibody solution having a known concentration.
  • the metal tube that forms the flow path prevents erosion by the antigen and residue of the antigen. Since the tube is made of metal, it is easily affected by the ambient temperature, but based on the detection result of the temperature sensor, the control unit operates the temperature controller to cool or heat the temperature inside the housing, for example, 25. Maintain a temperature within a predetermined range, such as around ° C.
  • the tube body forming the flow path is made of metal, and the inside of the housing is maintained at a desired temperature by a temperature controller, so that the inside of the tube body is prevented from being contaminated.
  • a temperature controller so that the inside of the tube body is prevented from being contaminated.
  • FIG. 1 Perspective view of the optical measuring device. Front view with the cover of the optical measuring device removed. Top view of the optical measuring device with the top plate removed. Sectional drawing of the main part of a temperature control mechanism. Block Diagram.
  • FIG. 1 shows a perspective view of an optical measuring device 11 (hereinafter referred to as “device”) for measuring the concentration of dioxins and PCBs (polychlorinated biphenyls).
  • device for measuring the concentration of dioxins and PCBs (polychlorinated biphenyls).
  • the apparatus 11 forms a complex by an antigen-antibody reaction between a sample containing an antigen such as dioxin and a fluorescently labeled antibody solution, and fills the antigen derivative immobilization carrier.
  • the unreacted antibody in the reaction solution is captured in the measurement cell, and the fluorescence intensity is measured (see Patent No. 4777983).
  • a sample with an unknown concentration of antigen and a fluorescently labeled antibody solution with a known concentration are mixed to prepare a sample solution for measurement, and this sample solution for measurement is sent to a measurement cell.
  • a complex is formed by the antigen-antibody reaction, and a certain equilibrium state is formed.
  • the unreacted antibody remaining in the measurement sample solution is captured by the antigen derivative immobilization carrier packed in the measurement cell.
  • the antibody-only solution (Bo solution) in which no sample is present will be captured in the largest amount.
  • the amount of antibody captured by the antigen derivative immobilization carrier is measured as the amount of fluorescence, and the concentration of the antigen is calculated from the ratio with the amount of fluorescence of the Bo solution.
  • the antibody is dissociated by flowing the regeneration solution into the measurement cell, and regeneration is performed to return to the state before the measurement.
  • regeneration is performed to return to the state before the measurement.
  • the device 11 that performs such a measurement has a substantially rectangular parallelepiped box-shaped housing 12, and the housing 12 has a support base 13 that protrudes forward at the lower end of the front surface. It has an eaves-shaped portion 14 protruding forward at the upper end of the front surface.
  • FIG. 2 is a front view of the apparatus 11 with the reagent cover 15 on the support base 13 and the sample cover 16 on the front removed
  • FIG. 3 is a plan view of the housing 12 with the top plate 17 removed.
  • the device 11 left and right the waste liquid bottle 18, the buffer liquid bottle 19 for measurement, the buffer waste liquid bottle 20, the regeneration solution bottle 21, the cleaning liquid bottle 22, and the sanitary liquid bottle 23 on the support base 13. They are arranged in order from one side in the direction.
  • the tube body 24 connected to the upper ends of the bottles 18, 19, 20, 21, 22, and 23 extends from the front plate 12a of the housing 12 into the housing 12.
  • a photoelectric cell mechanism 25 for detachably holding the above-mentioned measurement cell (not shown) is formed on the front plate 12a of the housing 12 and above the above-mentioned waste liquid bottle 18.
  • the photoelectric cell mechanism unit 25 is provided with an excitation light source, an optical sensor, and the like (not shown), and is a portion for measuring the amount of fluorescence.
  • a pump (not shown) including a metering pump such as a tube pump is connected to the photoelectric cell mechanism 25, and is configured to flow a measurement sample solution through the holding measurement cell.
  • excitation light sources are connected to a controller (not shown) and are driven and controlled by the controller.
  • a sample suction unit 26 is provided on the front plate 12a of the housing 12 at a position adjacent to the photoelectric cell mechanism unit 25.
  • a plurality of sample bottles 27 for storing the above-mentioned measurement sample solution are detachably held in the sample suction unit 26.
  • the sample suction unit 26 includes a sample bottle holder 28 that holds the sample bottle 27 detachably, a mounting portion 29 that detachably attaches the sample bottle holder 28, and a plurality of suction tubes 30 located above the mounting portion 29.
  • a suction pipe holding portion 31 is provided.
  • the attachment portion 29 and the suction pipe holding portion 31 are relatively movable, and are configured to approach and separate from each other.
  • the sample cover 16 described above covers the front surface of the suction tube holding portion 31.
  • a plurality of suction pipe connecting portions 32 connected to the suction pipe 30 are provided at the bottom of the eaves-shaped portion 14 of the housing 12 (see FIG. 3).
  • the suction pipe connecting portion 32 is provided with a suction flow path pipe 33 as one of the flow paths.
  • the suction flow path pipe 33 is connected to a direction switching valve 34 that switches the flow of a plurality of liquids.
  • Other flow path pipes 35 required for measurement processing are also connected to the suction flow path pipe 33.
  • the pipe bodies (suction flow path pipe 33, flow path pipe 33) that form the flow path through which the liquid required for the measurement process flows, including the above-mentioned pipe body 24 exposed on the front surface of the housing 12, are made of metal.
  • the metal is preferably made of copper. This is because copper has excellent corrosion resistance.
  • Such a housing 12 is provided with a temperature control mechanism as shown in FIGS. 3, 4, and 5.
  • the temperature control mechanism operates the temperature controller 41 that adjusts the temperature inside the housing 12, the temperature sensor 42 that measures the temperature inside the housing 12, and the temperature controller 41 based on the detection of the temperature sensor 42. It has a control unit 43 to operate.
  • the temperature controller 41 is configured by an appropriate means, and can be provided at an appropriate part according to the capacity and size.
  • a plate-shaped Peltier element 41a driven by PWM (Pulse Width Modulation) is preferably used. This is because the Peltier element 41a can be heated and cooled, and can be miniaturized, which is convenient.
  • the Peltier element 41a which is the temperature controller 41, may be provided on the top plate 17 constituting the upper end surface of the housing 12, for example.
  • the Peltier element 41a is provided in a part of the top plate 17 above the direction switching valve 34 described above (see the virtual line in FIG. 3).
  • FIG. 4 is an enlarged cross-sectional view showing a schematic structure of a portion centered on the temperature controller 41.
  • the Peltier element 41a is formed by an aluminum plate 44 that is substantially flush with the lower surface of the top plate 17. , Is fixed to the lower surface of the heat sink 45.
  • a frame body 46 having a heat insulating function is provided on the outer peripheral portion of the Peltier element 41a on the lower surface of the heat sink 45.
  • a cooling fan 47 is provided on the upper surface of the heat sink 45, which is the side having the fins 45a. Therefore, the portion of the top plate 17 accommodating the Peltier element 41a and the like has a bulging portion 17a that bulges upward.
  • the temperature sensor 42 described above is provided at an appropriate position in the housing 12.
  • the temperature sensor 42 is fixed to the vertical surface of the metal plate 48 at a position surrounding the direction switching valve 34 as shown in FIG.
  • the supplementary temperature control mechanism has a power supply 49 for supplying electric power, an input unit 50 for setting a set temperature, and a display unit 51 for displaying the temperature detected by the temperature sensor 42.
  • the temperature sensor 42, the Peltier element 41a, the cooling fan 47, the input unit 50, and the display unit 51 are connected to the control unit 43 and drive-controlled as shown in FIG. That is, the control unit 43 performs the control operation as follows according to a predetermined program.
  • control unit 43 When the control unit 43 inputs the detection result from the temperature sensor 42, the control unit 43 displays the detected temperature on the display unit 51 and compares the preset set temperature set in advance using the input unit 50.
  • control unit 43 compares the detected temperature of the temperature sensor 42 with the set temperature, and when it determines that temperature adjustment is necessary, applies power to the cooling fan 47 and the Peltier element 41a so that the set temperature is reached. Heating or cooling is performed by the Peltier element 41a. Heating or cooling is performed by reducing the temperature change by a pulse signal. As a result, the temperature inside the housing 12 is set to the set temperature, and electric power is applied to the cooling fan 47 and the Peltier element 41a so as to maintain the temperature.
  • the control unit 43 may display the detection temperature of the temperature sensor 42 and the target set temperature on the display unit 51, and may also display an operation permit meaning that measurement is possible. Further, the control unit 43 may be configured to transmit an operable signal to the controller of the device 11.
  • the temperature control mechanism is driven prior to the measurement process.
  • the control unit 43 adjusts the temperature based on the temperature sensor 42 and the set temperature, and the temperature inside the housing 12 is set to the set temperature, and this temperature is maintained. In this state, the measurement process is performed by the main body of the device 11.
  • the temperature control mechanism controls the temperature based on the temperature inside the housing 12, it also affects the temperature change outside, that is, the temperature change in the space where the device 11 is installed, and the temperature change caused by the pump or the like driven in the housing 12.
  • the temperature can be kept constant without being affected.
  • the temperature controller 41 of the temperature control mechanism is composed of the Peltier element 41a, it is possible to avoid an increase in the size of the temperature control mechanism. Further, by providing the Peltier element 41a on the top plate 17 as described above, it is possible to modify the device 11 into a device 11 capable of eliminating the variation in the measurement results as described above while leaving the portion other than the top plate of the existing device 11 as it is.
  • the Peltier element 41a is provided above the direction switching valve 34 to which the suction flow path tube 33 or the like through which the measurement sample solution flows is connected. Therefore, the temperature of the portion centering on the direction switching valve 34 that easily affects the measurement result can be appropriately maintained, so that the variation in the measurement result can be satisfactorily eliminated.
  • the pipe body that constitutes the flow path through which the liquid necessary for measurement such as the sample solution for measurement flows that is, the suction flow path pipe 33, the flow path pipe 35, and the pipe body 24 is made of a copper pipe, and thus has corrosion resistance. It is excellent in and can eliminate variations in measurement results due to contamination.
  • the tube is a copper tube, it has good heat transfer property and is easily affected by temperature changes.
  • the temperature control mechanism keeps the temperature inside the housing 12 constant regardless of the outside temperature. , It is possible to avoid variations in measurement results due to differences in temperature. In other words, highly accurate measurement can be performed in a temperature environment suitable for the measurement created by the temperature control mechanism.
  • Optical measuring device 12 Housing 17 ... Top plate 24 ... Tube 33 ... Suction flow path tube 34 ... Direction switching valve 35 ... Flow path tube 41 ... Temperature controller 41a ... Peltier element 42 ... Temperature sensor 43 ... Control unit

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Abstract

[Problem] To improve accuracy of measurement by removing variation in a measuring result in terms of the temperature of a measurement environment and cleanliness of a flow passage. [Solution] This optical measuring device is provided with, inside a housing 12, a flow passage for feeding a sample solution for measurement, and uses a fluid in which a complex is formed by an antigen-antibody reaction by adding, to the sample solution for measurement, a sample with an unknown antigen concentration and a fluorescently labeled an antibody solution with a known antibody concentration, the optical measuring device comprising: a Peltier element 41a for adjusting the temperature inside the housing 12; a temperature sensor for measuring the temperature inside the housing 12; and a control unit for operating the Peltier element 41a on the base of the detection by the temperature sensor. In addition, a pipe body constituting a flow passage for feeding the sample solution for measurement is composed of a copper pipe.

Description

光学測定装置Optical measuring device
　この発明は、例えばダイオキシン類やＰＣＢ（ポリ塩化ビフェニル）の濃度を蛍光検出法によって測定するような光学測定装置に関する。 The present invention relates to an optical measuring device for measuring the concentration of dioxins or PCB (polychlorinated biphenyl) by a fluorescence detection method, for example.
　前述のような光学測定装置として、下記特許文献１に開示されたものがある。この装置は、抗原であるダイオキシン類などを含有した試料と蛍光標識した抗体溶液との抗原抗体反応により複合体を形成させ、抗原誘導体固相化担体を充填した測定セルにて反応溶液中の未反応抗体を捕捉し、蛍光強度を測定するというものである。 As the optical measuring device as described above, there is one disclosed in Patent Document 1 below. This device forms a complex by an antigen-antibody reaction between a sample containing dioxin, which is an antigen, and a fluorescently labeled antibody solution, and is not yet in the reaction solution in a measurement cell filled with an antigen derivative immobilization carrier. The reaction antibody is captured and the fluorescence intensity is measured.
　装置の測定部には、前述の測定セルと、測定用試料溶液を測定セルの流路に流すポンプと、励起光源と、光センサと、コントローラを備えている。ポンプには、チューブポンプのような定量ポンプが用いられる。流路には、検査に必要な液体として、測定用試料溶液のほか、廃液、測定用バッファー液、再生液およびサニタリ液が流れる部分があり、流路の一部には、各液体の流路を切り換える流路切換弁が設けられている。 The measurement unit of the device is equipped with the above-mentioned measurement cell, a pump for flowing the measurement sample solution through the flow path of the measurement cell, an excitation light source, an optical sensor, and a controller. As the pump, a metering pump such as a tube pump is used. In addition to the sample solution for measurement, there is a part in the flow path through which waste liquid, buffer solution for measurement, regeneration liquid, and sanitary liquid flow, and part of the flow path is the flow path of each liquid. A flow path switching valve for switching between the two is provided.
　ポンプをはじめとして、流路の多くの部分や流路切換弁などは、筐体の内部に備えられている。また、流路は樹脂チューブで形成されている。 The pump, many parts of the flow path, the flow path switching valve, etc. are provided inside the housing. Further, the flow path is formed of a resin tube.
　このような装置は、測定環境の温度の違いによって測定結果にばらつきが生じるので、一定範囲の温度環境下で使用される。 Such a device is used in a certain temperature environment because the measurement result varies depending on the temperature of the measurement environment.
　前述のように測定用試料溶液が流れる流路は樹脂チューブで形成されているので、測定環境が所定の温度範囲内である限り、測定結果にばらつきは生じにくい。 As described above, the flow path through which the measurement sample solution flows is formed of a resin tube, so as long as the measurement environment is within a predetermined temperature range, the measurement results are unlikely to vary.
　しかし一方で、流路が樹脂チューブであるため、抗原による浸食や抗原の残留などいわゆるコンタミネーションによって、測定結果に誤差が生じるおそれがある。 However, on the other hand, since the flow path is a resin tube, there is a possibility that an error may occur in the measurement result due to so-called contamination such as erosion by the antigen or residue of the antigen.
特許第４７７７９８３号公報Japanese Patent No. 4777983
　この発明は、温度および流路の清浄度の点から測定精度を向上できるようにすることを主な目的とする。 The main object of the present invention is to improve the measurement accuracy in terms of temperature and cleanliness of the flow path.
　そのための手段は、筐体の内部に、測定用試料溶液を送る流路を備えており、前記測定用試料溶液に、抗原が未知濃度の試料と既知濃度の蛍光標識した抗体溶液を混合して抗原抗体反応による複合体が形成された液体を用いる光学測定装置であって、前記筐体の内部の温度を調節する温度調節器と、前記筐体の内部の温度を測定する温度センサと、前記温度センサの検知に基づいて前記温度調節器を作動する制御部を備えるとともに、前記流路を構成する管体が金属で形成された光学測定装置である。 As a means for that purpose, a flow path for sending the measurement sample solution is provided inside the housing, and the measurement sample solution is mixed with a sample having an unknown concentration of antigen and a fluorescently labeled antibody solution having a known concentration. An optical measuring device using a liquid in which a complex is formed by an antigen-antibody reaction, the temperature controller for adjusting the temperature inside the housing, a temperature sensor for measuring the temperature inside the housing, and the above. It is an optical measuring device including a control unit that operates the temperature controller based on the detection of the temperature sensor, and the tube body constituting the flow path is made of metal.
　この構成では、流路を形成する金属製の管体は、抗原による浸食や抗原の残留を防止する。管体は金属製であるため周囲の温度の影響を受けやすいが、温度センサの検知結果に基づいて制御部が温度調節器を作動して冷却または加温し、筐体内の温度を、例えば２５℃前後などのあらかじめ定められた範囲の温度に維持する。 In this configuration, the metal tube that forms the flow path prevents erosion by the antigen and residue of the antigen. Since the tube is made of metal, it is easily affected by the ambient temperature, but based on the detection result of the temperature sensor, the control unit operates the temperature controller to cool or heat the temperature inside the housing, for example, 25. Maintain a temperature within a predetermined range, such as around ° C.
　この発明によれば、流路を形成する管体を金属製にするとともに、筐体の内部を温度調節器によって所望の温度に維持するように構成したので、管体の内部の汚染が防止されるうえに、温度の違いによる測定結果のばらつきも抑えて、測定精度を高めることができる。 According to the present invention, the tube body forming the flow path is made of metal, and the inside of the housing is maintained at a desired temperature by a temperature controller, so that the inside of the tube body is prevented from being contaminated. In addition, it is possible to improve the measurement accuracy by suppressing the variation in the measurement result due to the difference in temperature.
光学測定装置の斜視図。Perspective view of the optical measuring device. 光学測定装置のカバーを外した状態の正面図。Front view with the cover of the optical measuring device removed. 光学測定装置の天板を外した状態の平面図。Top view of the optical measuring device with the top plate removed. 温度調節機構の要部の断面図。Sectional drawing of the main part of a temperature control mechanism. ブロック図。Block Diagram.
　この発明を実施するための一形態を、以下図面を用いて説明する。 A mode for carrying out the present invention will be described below with reference to the drawings.
　図１は、ダイオキシン類やＰＣＢ（ポリ塩化ビフェニル）の濃度を測定する光学測定装置１１（以下、「装置」という）の斜視図を示す。 FIG. 1 shows a perspective view of an optical measuring device 11 (hereinafter referred to as “device”) for measuring the concentration of dioxins and PCBs (polychlorinated biphenyls).
　装置１１は、背景技術の項目において述べたように、抗原であるダイオキシン類などを含有した試料と蛍光標識した抗体溶液との抗原抗体反応により複合体を形成させ、抗原誘導体固相化担体を充填した測定セルにて反応溶液中の未反応抗体を捕捉し、蛍光強度を測定するというものである（特許第４７７７９８３号参照）。 As described in the item of background art, the apparatus 11 forms a complex by an antigen-antibody reaction between a sample containing an antigen such as dioxin and a fluorescently labeled antibody solution, and fills the antigen derivative immobilization carrier. The unreacted antibody in the reaction solution is captured in the measurement cell, and the fluorescence intensity is measured (see Patent No. 4777983).
　より具体的には、抗原が未知濃度の試料と既知濃度の蛍光標識した抗体溶液を混ぜ合わせて測定用試料溶液を調製し、この測定用試料溶液を測定セルに送る。測定用試料溶液の中では、抗原抗体反応により複合体がつくられ、一定の平衡状態が形成されている。 More specifically, a sample with an unknown concentration of antigen and a fluorescently labeled antibody solution with a known concentration are mixed to prepare a sample solution for measurement, and this sample solution for measurement is sent to a measurement cell. In the sample solution for measurement, a complex is formed by the antigen-antibody reaction, and a certain equilibrium state is formed.
　測定用試料溶液を測定セルに送ると、測定用試料溶液中に残っている未反応抗体が測定セルに充填された抗原誘導体固相化担体に捕捉される。捕捉される量は、試料が存在しない抗体のみの溶液（Ｂｏ溶液）が最も多く捕捉されることになる。 When the measurement sample solution is sent to the measurement cell, the unreacted antibody remaining in the measurement sample solution is captured by the antigen derivative immobilization carrier packed in the measurement cell. As for the amount to be captured, the antibody-only solution (Bo solution) in which no sample is present will be captured in the largest amount.
　抗原誘導体固相化担体に捕捉された抗体の量は蛍光量として測定され、Ｂｏ溶液の蛍光量との比から抗原の濃度が算出される。 The amount of antibody captured by the antigen derivative immobilization carrier is measured as the amount of fluorescence, and the concentration of the antigen is calculated from the ratio with the amount of fluorescence of the Bo solution.
　測定終了後は、測定セルに再生溶液を流すことで抗体を解離させて、測定前の状態に戻す再生がなされる。測定と再生の繰り返しによって、同一の測定セルで複数回の測定を行うことができる。 After the measurement is completed, the antibody is dissociated by flowing the regeneration solution into the measurement cell, and regeneration is performed to return to the state before the measurement. By repeating measurement and reproduction, multiple measurements can be performed in the same measurement cell.
　おおよそこのような測定を行う装置１１は、図１に示したように、略直方体箱状の筐体１２を有し、筐体１２は正面の下端部に前方にせり出す支持台１３を有し、正面の上端に前方にせり出す庇状部１４を有している。 As shown in FIG. 1, the device 11 that performs such a measurement has a substantially rectangular parallelepiped box-shaped housing 12, and the housing 12 has a support base 13 that protrudes forward at the lower end of the front surface. It has an eaves-shaped portion 14 protruding forward at the upper end of the front surface.
　図２は、装置１１から支持台１３上の試薬カバー１５と正面の試料カバー１６を外した状態の正面図、図３は筐体１２の天板１７を外した状態の平面図である。これらの図に示すように、装置１１は、支持台１３の上に、廃液瓶１８、測定用バッファー液瓶１９、バッファー廃液瓶２０、再生溶液瓶２１、洗浄液瓶２２、サニタリ液瓶２３を左右方向の一方側から順に配設している。各瓶１８，１９，２０，２１，２２，２３の上端に接続される管体２４は、筐体１２の正面板１２ａから筐体１２内に延びている。 FIG. 2 is a front view of the apparatus 11 with the reagent cover 15 on the support base 13 and the sample cover 16 on the front removed, and FIG. 3 is a plan view of the housing 12 with the top plate 17 removed. As shown in these figures, the device 11 left and right the waste liquid bottle 18, the buffer liquid bottle 19 for measurement, the buffer waste liquid bottle 20, the regeneration solution bottle 21, the cleaning liquid bottle 22, and the sanitary liquid bottle 23 on the support base 13. They are arranged in order from one side in the direction. The tube body 24 connected to the upper ends of the bottles 18, 19, 20, 21, 22, and 23 extends from the front plate 12a of the housing 12 into the housing 12.
　筐体１２の正面板１２ａ上であって、前述した廃液瓶１８の上方位置には、前述した測定セル（図示せず）を着脱可能に保持する光電セル機構部２５が形成されている。光電セル機構部２５は、励起光源や光センサ等（図示せず）を備えて、蛍光量の測定を行う部位である。光電セル機構部２５には、チューブポンプのような定量ポンプからなるポンプ（図示せず）が接続され、保持した測定セルに測定用試料溶液を流すように構成されている。 A photoelectric cell mechanism 25 for detachably holding the above-mentioned measurement cell (not shown) is formed on the front plate 12a of the housing 12 and above the above-mentioned waste liquid bottle 18. The photoelectric cell mechanism unit 25 is provided with an excitation light source, an optical sensor, and the like (not shown), and is a portion for measuring the amount of fluorescence. A pump (not shown) including a metering pump such as a tube pump is connected to the photoelectric cell mechanism 25, and is configured to flow a measurement sample solution through the holding measurement cell.
　これら励起光源や光センサ、ポンプは、コントローラ（図示せず）に接続されており、コントローラによって駆動制御される。 These excitation light sources, optical sensors, and pumps are connected to a controller (not shown) and are driven and controlled by the controller.
　筐体１２の正面板１２ａ上であって、光電セル機構部２５の隣接位置には、試料吸引部２６が設けられている。試料吸引部２６には、前述した測定用試料溶液を貯留する複数の試料瓶２７が着脱可能に保持される。 A sample suction unit 26 is provided on the front plate 12a of the housing 12 at a position adjacent to the photoelectric cell mechanism unit 25. A plurality of sample bottles 27 for storing the above-mentioned measurement sample solution are detachably held in the sample suction unit 26.
　試料吸引部２６には、試料瓶２７を着脱可能に保持する試料瓶ホルダ２８と、試料瓶ホルダ２８を着脱可能に取り付ける取り付け部２９と、取り付け部２９の上方に位置して複数の吸引管３０を垂設した吸引管保持部３１が備えられている。取り付け部２９と吸引管保持部３１は相対移動可能であり、互いに接近し、また離反する構成である。前述した試料カバー１６は、吸引管保持部３１の前面を覆うものである。 The sample suction unit 26 includes a sample bottle holder 28 that holds the sample bottle 27 detachably, a mounting portion 29 that detachably attaches the sample bottle holder 28, and a plurality of suction tubes 30 located above the mounting portion 29. A suction pipe holding portion 31 is provided. The attachment portion 29 and the suction pipe holding portion 31 are relatively movable, and are configured to approach and separate from each other. The sample cover 16 described above covers the front surface of the suction tube holding portion 31.
　筐体１２の庇状部１４の底部には、吸引管３０に接続される複数の吸引管接続部３２が設けられる（図３参照）。吸引管接続部３２には、流路の一つとしての吸引流路管３３がそれぞれ設けられている。 A plurality of suction pipe connecting portions 32 connected to the suction pipe 30 are provided at the bottom of the eaves-shaped portion 14 of the housing 12 (see FIG. 3). The suction pipe connecting portion 32 is provided with a suction flow path pipe 33 as one of the flow paths.
　吸引流路管３３は、複数の液体の流れを切り換える方向切換弁３４に接続されている。吸引流路管３３には、測定処理に必要なその他の流路管３５も接続されている。 The suction flow path pipe 33 is connected to a direction switching valve 34 that switches the flow of a plurality of liquids. Other flow path pipes 35 required for measurement processing are also connected to the suction flow path pipe 33.
　筐体１２の前面に露出する前述した管体２４を含めて測定処理に必要な液体が流れる流路を形成する管体（吸引流路管３３、流路管３３）は、金属製である。金属としては、銅製のものが好ましい。銅は耐食性に優れているからである。 The pipe bodies (suction flow path pipe 33, flow path pipe 33) that form the flow path through which the liquid required for the measurement process flows, including the above-mentioned pipe body 24 exposed on the front surface of the housing 12, are made of metal. The metal is preferably made of copper. This is because copper has excellent corrosion resistance.
　筐体１２の内部には、前述した各部材のほか、その他の必要な部材や機器が収納されている。 In addition to the above-mentioned members, other necessary members and devices are housed inside the housing 12.
　このような筐体１２には、図３、図４、図５に示したように、温度調節機構が備えられる。温度調節機構は、筐体１２の内部の温度を調節する温度調節器４１と、筐体１２の内部の温度を測定する温度センサ４２と、温度センサ４２の検知に基づいて温度調節器４１を作動する制御部４３を有する。 Such a housing 12 is provided with a temperature control mechanism as shown in FIGS. 3, 4, and 5. The temperature control mechanism operates the temperature controller 41 that adjusts the temperature inside the housing 12, the temperature sensor 42 that measures the temperature inside the housing 12, and the temperature controller 41 based on the detection of the temperature sensor 42. It has a control unit 43 to operate.
　温度調節器４１は、適宜の手段で構成され、能力や大きさに応じて適宜の部位に設けることができる。温度調節器４１としては、ＰＷＭ（Ｐｕｌｓｅ　Ｗｉｄｔｈ　Ｍｏｄｕｌａｔｉｏｎ）で駆動する板状のペルチェ素子４１ａが好適に用いられる。このようなペルチェ素子４１ａを用いると、加熱も冷却も可能であり、小型化等も可能であり便利だからである。 The temperature controller 41 is configured by an appropriate means, and can be provided at an appropriate part according to the capacity and size. As the temperature controller 41, a plate-shaped Peltier element 41a driven by PWM (Pulse Width Modulation) is preferably used. This is because the Peltier element 41a can be heated and cooled, and can be miniaturized, which is convenient.
　温度調節器４１であるペルチェ素子４１ａは、例えば筐体１２の上端面を構成する天板１７に設けるとよい。ペルチェ素子４１ａは、天板１７の一部でも特に、前述した方向切換弁３４の上方に備えられる（図３の仮想線参照）。 The Peltier element 41a, which is the temperature controller 41, may be provided on the top plate 17 constituting the upper end surface of the housing 12, for example. The Peltier element 41a is provided in a part of the top plate 17 above the direction switching valve 34 described above (see the virtual line in FIG. 3).
　図４は、温度調節器４１を中心とした部分の概略構造を示す拡大断面図であり、この図に示すようにペルチェ素子４１ａは、天板１７の下面と略面一になるアルミ板４４によって、ヒートシンク４５の下面に固定されている。ヒートシンク４５の下面におけるペルチェ素子４１ａの外周部には断熱機能を有する枠体４６が設けられる。ヒートシンク４５におけるフィン４５ａを有する側である上面には、冷却ファン４７が設けられている。このため天板１７におけるペルチェ素子４１ａ等を収容する部分は、上方へ膨出する膨出部１７ａを有している。 FIG. 4 is an enlarged cross-sectional view showing a schematic structure of a portion centered on the temperature controller 41. As shown in this figure, the Peltier element 41a is formed by an aluminum plate 44 that is substantially flush with the lower surface of the top plate 17. , Is fixed to the lower surface of the heat sink 45. A frame body 46 having a heat insulating function is provided on the outer peripheral portion of the Peltier element 41a on the lower surface of the heat sink 45. A cooling fan 47 is provided on the upper surface of the heat sink 45, which is the side having the fins 45a. Therefore, the portion of the top plate 17 accommodating the Peltier element 41a and the like has a bulging portion 17a that bulges upward.
　また、前述の温度センサ４２は筐体１２内における適宜位置に設けられる。図示例では、温度センサ４２は、図３に示したように方向切換弁３４を囲む位置にある金属板４８の垂直な面に固定されている。 Further, the temperature sensor 42 described above is provided at an appropriate position in the housing 12. In the illustrated example, the temperature sensor 42 is fixed to the vertical surface of the metal plate 48 at a position surrounding the direction switching valve 34 as shown in FIG.
　これらのほか、補温度調節機構は、電力を供給する電源４９と、設定温度を設定するための入力部５０と、温度センサ４２が検知した温度などを表示する表示部５１を有している。 In addition to these, the supplementary temperature control mechanism has a power supply 49 for supplying electric power, an input unit 50 for setting a set temperature, and a display unit 51 for displaying the temperature detected by the temperature sensor 42.
　これら温度センサ４２と、ペルチェ素子４１ａと、冷却ファン４７と、入力部５０と、表示部５１は、図５に示したように制御部４３に接続されて駆動制御される。すなわち、制御部４３はあらかじめ定めらたれプログラムに従ってつぎのように制御動作を行う。 The temperature sensor 42, the Peltier element 41a, the cooling fan 47, the input unit 50, and the display unit 51 are connected to the control unit 43 and drive-controlled as shown in FIG. That is, the control unit 43 performs the control operation as follows according to a predetermined program.
　制御部４３は、温度センサ４２から検知結果を入力すると、その検出温度を表示部５１に表示するとともに、入力部５０を用いてあらかじめ設定された、目標である設定温度を比較する。 When the control unit 43 inputs the detection result from the temperature sensor 42, the control unit 43 displays the detected temperature on the display unit 51 and compares the preset set temperature set in advance using the input unit 50.
　そして制御部４３は、温度センサ４２の検出温度と設定温度を対比して、温度調節が必要と判断した場合に、冷却ファン４７とペルチェ素子４１ａに電力を印加して、設定温度になるようにペルチェ素子４１ａによる加熱または冷却を行う。加熱または冷却は、パルス信号により温度変化を少なくして行われる。これによって、筐体１２内の温度を設定温度にするとともに、その温度を維持するように冷却ファン４７とペルチェ素子４１ａに電力を印加する。 Then, the control unit 43 compares the detected temperature of the temperature sensor 42 with the set temperature, and when it determines that temperature adjustment is necessary, applies power to the cooling fan 47 and the Peltier element 41a so that the set temperature is reached. Heating or cooling is performed by the Peltier element 41a. Heating or cooling is performed by reducing the temperature change by a pulse signal. As a result, the temperature inside the housing 12 is set to the set temperature, and electric power is applied to the cooling fan 47 and the Peltier element 41a so as to maintain the temperature.
　制御部４３は、表示部５１に温度センサ４２の検出温度と、目標の設定温度を表示するほか、測定可能を意味する運転許可の表示をしてもよい。また、制御部４３が装置１１のコントローラに運転可能信号を送信するように構成してもよい。 The control unit 43 may display the detection temperature of the temperature sensor 42 and the target set temperature on the display unit 51, and may also display an operation permit meaning that measurement is possible. Further, the control unit 43 may be configured to transmit an operable signal to the controller of the device 11.
　以上のように構成された装置１１では、測定処理に先立って温度調節機構を駆動する。温度調節機構では、制御部４３により温度センサ４２と設定温度に基づいた温度調節がなされて、筐体１２内の温度が設定温度にされて、この温度が維持される。この状態で、装置１１の本体部による測定処理がなされる。 In the device 11 configured as described above, the temperature control mechanism is driven prior to the measurement process. In the temperature control mechanism, the control unit 43 adjusts the temperature based on the temperature sensor 42 and the set temperature, and the temperature inside the housing 12 is set to the set temperature, and this temperature is maintained. In this state, the measurement process is performed by the main body of the device 11.
　このため、測定時の周囲の温度の違いによる測定結果のばらつきを解消できる。温度調節機構は筐体１２内の温度に基づいて温度調節を行うので、外部、つまり装置１１を設置した空間の温度変化や、筐体１２内で駆動するポンプ等に起因する温度変化にも影響を受けずに、温度を一定に保つことができる。 Therefore, it is possible to eliminate variations in measurement results due to differences in ambient temperature during measurement. Since the temperature control mechanism controls the temperature based on the temperature inside the housing 12, it also affects the temperature change outside, that is, the temperature change in the space where the device 11 is installed, and the temperature change caused by the pump or the like driven in the housing 12. The temperature can be kept constant without being affected.
　しかも、温度調節機構の温度調節器４１はペルチェ素子４１ａで構成しているので、温度調節機構の大型化を回避できる。またペルチェ素子４１ａを前述のように天板１７に備えることで、既存の装置１１の天板以外の部分をそのままにして、前述のような測定結果のばらつきを解消できる装置１１に改変できる。 Moreover, since the temperature controller 41 of the temperature control mechanism is composed of the Peltier element 41a, it is possible to avoid an increase in the size of the temperature control mechanism. Further, by providing the Peltier element 41a on the top plate 17 as described above, it is possible to modify the device 11 into a device 11 capable of eliminating the variation in the measurement results as described above while leaving the portion other than the top plate of the existing device 11 as it is.
　また、ペルチェ素子４１ａは、測定用試料溶液を流す吸引流路管３３等が接続された方向切換弁３４の上方に備えられている。このため、方向切換弁３４を中心とした測定結果に影響を及ぼしやすい部分の温度を適切に維持することができるので、測定結果のばらつきを良好に解消できる。 Further, the Peltier element 41a is provided above the direction switching valve 34 to which the suction flow path tube 33 or the like through which the measurement sample solution flows is connected. Therefore, the temperature of the portion centering on the direction switching valve 34 that easily affects the measurement result can be appropriately maintained, so that the variation in the measurement result can be satisfactorily eliminated.
　そのうえ、測定用試料溶液等の測定に必要な液体を流す流路を構成する管体、つまり吸引流路管３３や流路管３５、管体２４は、銅管で構成されているので、耐食性にすぐれており、コンタミネーションによる測定結果のばらつきも解消できる。 In addition, the pipe body that constitutes the flow path through which the liquid necessary for measurement such as the sample solution for measurement flows, that is, the suction flow path pipe 33, the flow path pipe 35, and the pipe body 24 is made of a copper pipe, and thus has corrosion resistance. It is excellent in and can eliminate variations in measurement results due to contamination.
　管体が銅管であると熱伝達性が良いので、温度変化の影響を受けやすいが、前述のように温度調節機構が筐体１２内の温度を外部の温度に関わりなく一定に維持するので、温度の違いによる測定結果のばらつきが生じることは回避できる。換言すれば、温度調節機構により作出した測定に適した温度環境下で精度の高い測定が行える。 If the tube is a copper tube, it has good heat transfer property and is easily affected by temperature changes. However, as described above, the temperature control mechanism keeps the temperature inside the housing 12 constant regardless of the outside temperature. , It is possible to avoid variations in measurement results due to differences in temperature. In other words, highly accurate measurement can be performed in a temperature environment suitable for the measurement created by the temperature control mechanism.
　以上のように、この発明の装置１１を用いた測定によって、精度の高い測定結果が得られる。 As described above, highly accurate measurement results can be obtained by measurement using the device 11 of the present invention.
　１１…光学測定装置
　１２…筐体
　１７…天板
　２４…管体
　３３…吸引流路管
　３４…方向切換弁
　３５…流路管
　４１…温度調節器
　４１ａ…ペルチェ素子
　４２…温度センサ
　４３…制御部 11 ... Optical measuring device 12 ... Housing 17 ... Top plate 24 ... Tube 33 ... Suction flow path tube 34 ... Direction switching valve 35 ... Flow path tube 41 ... Temperature controller 41a ... Peltier element 42 ... Temperature sensor 43 ... Control unit

Claims (4)

  1. 筐体の内部に、測定用試料溶液を送る流路を備えており、前記測定用試料溶液に、抗原が未知濃度の試料と既知濃度の蛍光標識した抗体溶液を混合して抗原抗体反応による複合体が形成された液体を用いる光学測定装置であって、
    前記筐体の内部の温度を調節する温度調節器と、
    前記筐体の内部の温度を測定する温度センサと、
    前記温度センサの検知に基づいて前記温度調節器を作動する制御部を備えるとともに、
    前記流路を構成する管体が金属で形成された
    光学測定装置。     A flow path for sending a sample solution for measurement is provided inside the housing, and a sample having an unknown concentration of antigen and a fluorescently labeled antibody solution having a known concentration are mixed with the sample solution for measurement and combined by an antigen-antibody reaction. An optical measuring device that uses a liquid in which a body is formed.
    A temperature controller that regulates the temperature inside the housing,
    A temperature sensor that measures the temperature inside the housing and
    In addition to being provided with a control unit that operates the temperature controller based on the detection of the temperature sensor,
    An optical measuring device in which the tube body constituting the flow path is made of metal.
  2. 前記温度調節器がペルチェ素子である
    請求項１に記載の光学測定装置。     The optical measuring device according to claim 1, wherein the temperature controller is a Peltier element.
  3. 前記温度調節器が、前記筐体の天板に設けられた
    請求項１または請求項２に記載の光学測定装置。     The optical measuring device according to claim 1 or 2, wherein the temperature controller is provided on the top plate of the housing.
  4. 前記流路の一部に、複数の前記液体の流れを切り換える方向切換弁が設けられ、
    前記方向切換弁の上方に、前記温度調節器が備えられた
    請求項１から請求項３のうちいずれか一項に記載の光学測定装置。     A direction switching valve for switching the flow of a plurality of the liquids is provided in a part of the flow path.
    The optical measuring device according to any one of claims 1 to 3, wherein the temperature controller is provided above the direction switching valve.
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