WO2020203510A1 - Appareil pour commander une combinaison d'un microcircuit et d'une seringue - Google Patents

Appareil pour commander une combinaison d'un microcircuit et d'une seringue Download PDF

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Publication number
WO2020203510A1
WO2020203510A1 PCT/JP2020/013180 JP2020013180W WO2020203510A1 WO 2020203510 A1 WO2020203510 A1 WO 2020203510A1 JP 2020013180 W JP2020013180 W JP 2020013180W WO 2020203510 A1 WO2020203510 A1 WO 2020203510A1
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WO
WIPO (PCT)
Prior art keywords
syringe
blood
microcircuit
piston
cells
Prior art date
Application number
PCT/JP2020/013180
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English (en)
Japanese (ja)
Inventor
知大 久保
まどか 綾野
Original Assignee
株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2020020665A external-priority patent/JP2020162585A/ja
Application filed by 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ filed Critical 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ
Publication of WO2020203510A1 publication Critical patent/WO2020203510A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
    • B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

Definitions

  • the present invention relates to a driving device for a combination of a microcircuit and a syringe, and particularly to a device for classifying cells.
  • Patent Document 1 discloses a fractionator for biological components. This fractionator comprises a cartridge with a separation membrane. This fractionator drives the flow path in the cartridge with a tube pump.
  • Patent Document 2 discloses a microcircuit for hydraulically classifying cells.
  • Patent Document 3 discloses an injection device for a microreactor, which comprises a syringe, a magnetic rotating member arranged in the syringe, and a magnetic rotating power unit capable of rotating the magnetic rotating member from the outside of the syringe without contact. are doing.
  • the fine particles that can be used in the injection device for the microreactor include resin fine particles, inorganic fine particles, metal fine particles, ceramic fine particles, and the like (paragraph [0027]).
  • JP-A-2007-240304 Japanese Unexamined Patent Publication No. 2007-175684 JP-A-2007-000719
  • An object of the present invention is to provide a device that drives a combination of a microcircuit and a syringe in order to hydraulically classify cells in blood.
  • a driving device for driving a fluid device is A microcircuit for hydraulically classifying cells in blood, Blood intake and Syringe A for taking in and storing blood, A branch connecting the microcircuit, the intake, and the syringe A on three sides, A syringe B for storing the pressing liquid, which is connected to the microcircuit, and With The drive device
  • the reciprocating mechanism includes a reciprocating mechanism, a pressing mechanism, a stirring mechanism, and a control unit for controlling their coordinated operation. The reciprocating mechanism pulls the piston A of the syringe A from the intake via the branch.
  • the stirring mechanism has a magnet, and while blood is stored in the syringe A, the magnet moves a stirrer arranged in the syringe A from the outside of the syringe A, thereby causing the syringe. Stir the blood in A, Drive device.
  • the driving device further includes a mounting surface on which the reciprocating mechanism and the pressing mechanism are arranged.
  • the fluid device comprises a flat cartridge that is attached to the attachment surface when classifying cells in blood and is removed from the attachment surface after the blood has been classified.
  • the microcircuit, the intake, the syringe A, the branch, and the syringe B are arranged in the cartridge.
  • the back of the cartridge is oriented so that it faces the mounting surface of the fluid device.
  • the magnet is arranged so as to face the syringe A and move on the mounting surface.
  • the reciprocating mechanism is a connecting portion A that grips the piston rod of the piston A and an actuator A that reciprocates the connecting portion A along the longitudinal direction of the syringe A and connects to the control unit. And a hold portion for gripping the cylinder of the piston A.
  • the actuator A is arranged behind the magnet.
  • the drive device according to ⁇ 2>.
  • ⁇ 4> The magnet is a permanent magnet that reciprocates the stirrer in the syringe A along the longitudinal direction of the syringe A.
  • the stirring mechanism is an arm that holds the magnet from the side with respect to the reciprocating direction of the magnet, and an actuator M that reciprocates the arm along the longitudinal direction of the syringe A and is connected to the control unit.
  • the drive device When the fluid device is attached to the drive device, the syringe A stands with the discharge port down and the piston A up.
  • the hold portion of the reciprocating mechanism is arranged above the magnet.
  • the connecting portion A of the reciprocating mechanism is arranged above the holding portion.
  • the stirring mechanism directs the reciprocating magnet to the lower end of the cylinder of the syringe A.
  • the drive device ⁇ 4>.
  • ⁇ 6> When the control unit starts to take blood into the syringe A, the control unit starts to have the stirring mechanism stir the blood in the syringe A with the magnet.
  • the drive device according to ⁇ 1>.
  • the fluid device is A first conduit having a side having the intake and a side connecting to the branch, A second conduit located between the syringe A and the branch, A third conduit located between the branch and the microcircuit, With more
  • the drive device An intake valve that closes and opens the first conduit, A start valve that closes and opens the third conduit, With more
  • the control unit causes the intake valve to open the first conduit and the start valve to close the third conduit when the reciprocating mechanism pulls the piston A.
  • the control unit causes the intake valve to close the first conduit and the start valve to open the third conduit when the reciprocating mechanism pushes the piston A.
  • the control unit stores the program of the cooperative operation, and automatically executes the cooperative operation according to a time schedule derived from the program.
  • the drive device according to ⁇ 1> is provided. Further comprising the fluid device.
  • ⁇ 10> A method for classifying cells in blood with the separation device according to ⁇ 9>. In advance The diluent and the stirrer are placed in the syringe A. A pressing liquid is placed in the syringe B, A blood collection tube that stores blood is attached to the intake, The branch and the syringe B are connected to the upper surface of the microcircuit, and the branch is connected to the syringe B.
  • the blood flow is pushed to the opposite side.
  • the cells in the blood flow are hydraulically classified on the side not in contact with the flow of the pressing liquid, and the cells classified here are blood cells or floating cells that are not blood cells.
  • the classified cells are taken out from the outlet on the lower surface of the microcircuit.
  • a drive device for driving a combination of a fine circuit for hydraulically classifying cells in blood, a reservoir for storing pre-classified blood, and a syringe.
  • the syringe comprises at least a syringe A for taking in and storing blood and a syringe B for storing pressing fluid and connected to another inlet of the microcircuit.
  • the drive device A reciprocating mechanism, a mounting mechanism, a pressing mechanism, a stirring mechanism, and a control unit for controlling their cooperative operation are provided.
  • the reciprocating mechanism is provided in the syringe A by pulling the piston A of the syringe A.
  • the mounting mechanism mounts the discharge port of the syringe A at the inlet of the microcircuit.
  • the reciprocating mechanism continuously flows blood from the syringe A toward the microcircuit via the discharge port and the inlet.
  • the pressing mechanism the pressing liquid is continuously poured from the syringe B toward the microcircuit by pushing the piston B of the syringe B in accordance with the pushing of the piston A by the reciprocating mechanism.
  • the stirring mechanism has a magnet, and while blood is stored in the syringe A, the magnet moves a stirrer arranged in the syringe A from the outside of the syringe A, thereby causing the syringe.
  • the stirring mechanism By moving the stirrer arranged in the syringe A from the outside of the syringe A by the stirring mechanism, the blood in the syringe A and the diluent are mixed and stirred. Further, by pushing the piston A, diluted blood is continuously poured from the syringe A toward the microcircuit via the discharge port and the inlet. Further, as the reciprocating mechanism pushes the piston A, the pressing mechanism pushes the piston B of the syringe B to continuously flow the pressing liquid from the syringe B toward the microcircuit.
  • the blood flow is pushed to the opposite side. Further, the cells in the blood flow are hydraulically classified on the side not in contact with the flow of the pressing liquid, and the cells classified here are blood cells or floating cells that are not blood cells. The classified cells are taken out from the outlet on the lower surface of the microcircuit.
  • FIG. 1 Front view of the drive unit.
  • Usage diagram of drive and fluid device Usage diagram of drive and fluid device.
  • Usage diagram of drive and fluid device Usage diagram of drive and fluid device.
  • Perspective view of drive and fluid device Left side view of magnet and reciprocating mechanism.
  • Front view of the stirring mechanism and syringe A.
  • Top view of the microcircuit Partial plan view of the microcircuit. Detailed partial perspective view of the microcircuit.
  • Usage diagram of drive and fluid device Schematic diagram of the control unit. Front view of the drive unit.
  • FIG. 1 shows a drive device 70 for driving the fluid device 30.
  • the figure virtually represents a state in which the fluid device 30 is detached from the drive device 70.
  • the left side of the figure mainly represents the drive device 70.
  • the right side of the figure mainly represents the fluid device 30.
  • the fluid device 30 will be described before the drive device 70 is described.
  • the fluid device 30 includes a microcircuit 20.
  • a fine circuit is a chip having a fine fluid circuit.
  • the fine circuit may be identified by a reference numeral F (fine hydraulic circuit).
  • the microcircuit 20 hydraulically classifies the cells in the blood poured into the microcircuit 20.
  • the fluid device 30 includes a piping system 10.
  • the piping system 10 includes an intake port 50.
  • a blood collection tube 51 that stores blood, for example, whole blood, is connected to the intake port 50.
  • the fluid device 30 takes in blood from the blood collection tube 51 connected to the intake 50.
  • the fluid device 30 includes a syringe A for storing blood.
  • the syringe A and related members may be identified by reference numeral A.
  • the syringe that serves as the syringe A is the syringe 35a.
  • the syringe 35a takes blood into the syringe 35a and stores the blood in the syringe 35a.
  • the syringe 35a stores the diluted blood DB.
  • the syringe 35a includes a piston 32a that hits the piston A.
  • the syringe 35a further comprises a cylinder 34a.
  • the piston 32a reciprocates in the cylinder 34a.
  • the cylinder 34a has a discharge port 31a.
  • the cylinder 34a has a flange 36a.
  • the flange 36a is located on the opposite side of the discharge port 31a.
  • a stirrer 75 is arranged in the cylinder 34a.
  • the syringe 35a has a rod 37a.
  • the rod 37a is a piston rod of the syringe 35a.
  • One end of the rod 37a connects to the piston 32a.
  • the fluid device 30 further includes a syringe B for storing the pressing liquid.
  • a syringe B for storing the pressing liquid.
  • the syringe B and related members may be identified by reference numeral B.
  • the syringe that plays the role of syringe B is syringe 35b.
  • the syringe 35b stores the pressing liquid PL.
  • the second syringe 35b is connected to the microcircuit 20 via the fourth conduit 12.
  • the syringe 35b includes a piston 32b that hits the piston B.
  • the syringe 35b further comprises a cylinder 34b.
  • the piston 32b reciprocates in the cylinder 34b.
  • the cylinder 34b has a discharge port 31b.
  • the cylinder 34b has a flange 36b.
  • the flange 36b is located on the opposite side of the discharge port 31b.
  • the syringe 35b has a rod 37b.
  • the rod 37b is a piston rod of the syringe 35b. One end of the rod 37b connects to the piston 32b.
  • the piping system 10 includes a first conduit 11a, a second conduit 11b, a third conduit 11c, a fourth conduit 12, and a branch 15.
  • the branch 15 communicates between the microcircuit 20, the intake 50 and the syringe 35a on three sides.
  • the first conduit 11a has an end on the side having the intake 50 and an end on the side connecting to the branch 15.
  • the second conduit 11b is located between the syringe 35a and the branch 15.
  • the third conduit 11c is located between the branch 15 and the microcircuit 20.
  • the second conduit 11b is located between the syringe 35a and the branch 15. One end of the second conduit 11b is connected to the discharge port 31a. The other end of the second conduit 11b connects to the branch 15.
  • the third conduit 11c is located between the branch 15 and the microcircuit 20.
  • One end of the third conduit 11c connects to the branch 15.
  • the other end of the third conduit 11c connects to the microcircuit 20.
  • the fourth conduit 12 is located between the syringe 35b and the microcircuit 20. One end of the fourth conduit 12 is connected to the discharge port 31b. The other end of the fourth conduit 12 connects to the microcircuit 20.
  • the blood collection tube 51 has a cap 52.
  • the cap 52 may be a rubber stopper.
  • the ventilation needle 53 may be attached to the blood collection tube 51.
  • the ventilation needle 53 penetrates the cap 52.
  • the air outside the blood collection tube 51 is guided to the lumen of the blood collection tube 51.
  • the blood collection tube 51 is housed in the blood collection tube holder 54.
  • the intake 50 may be connected to the blood collection tube 51 via the blood collection tube holder 54.
  • the drive device 70 and the fluid device 30 can be combined to form an integrated separation device.
  • a separator with a drive device 70 separates the target cell from other cells or components in the blood.
  • the drive device 70 includes a reciprocating mechanism 71a, a pressing mechanism 71b, and a stirring mechanism 71c.
  • the drive device 70 further includes a control unit 80 that controls these coordinated operations.
  • the reciprocating mechanism 71a includes a grip portion 72a that corresponds to the connecting portion A.
  • the reciprocating mechanism 71a further includes an actuator 73a that corresponds to the actuator A. Since the reciprocating mechanism 71a is related to the syringe A, the reciprocating mechanism may be identified by the reference numeral A below.
  • the pressing mechanism 71b includes a grip portion 72b.
  • the pressing mechanism 71b further includes an actuator 73b. Since the pressing mechanism 71b is related to the syringe B, the pressing mechanism may be identified by the reference numeral B below.
  • the stirring mechanism 71c includes an arm 72c, an actuator 73c corresponding to the actuator M, and a magnet 74.
  • the stirring mechanism 71c may be identified by a reference numeral M (mixing).
  • the arm 72c holds the magnet 74.
  • the actuator 73c moves the magnet 74 via the arm 72c.
  • the mode in which the stirring mechanism 71c moves the magnet 74 is not limited to this.
  • the magnet 74 may be a permanent magnet or an electromagnet.
  • the stirring mechanism 71c stirs the diluted blood DB in the cylinder 34a with the stirrer 75.
  • the stirrer 75 has a ferromagnet.
  • the magnetic force of the magnet 74 affects the stirrer 75.
  • the magnet 74 moves the stirrer 75 while blood is stored in the cylinder 34a as the diluted blood DB.
  • the stirring mechanism 71c can continuously stir the diluted blood DB to suppress the precipitation of blood cells or floating cells that are not blood cells in the diluted blood DB.
  • control unit 80 is connected to the actuator 73a, the actuator 73b, and the actuator 73c.
  • the control unit 80 causes the stirring mechanism 71c to cooperate with the reciprocating mechanism 71a and the pressing mechanism 71b via these actuators.
  • the drive device 70 includes an intake valve 76a and a start valve 76b.
  • the control unit 80 connects to these valves. Under the control of the control unit 80, the intake valve 76a can close and open the first conduit 11a. Under control by the control unit 80, the start valve 76b can close and open the third conduit 11c.
  • FIG. 2 to 4 show how the fluid device 30 is operated by using the drive device 70. First, how the drive device 70 and the fluid device 30 are combined will be described with reference to FIG.
  • FIG. 2 shows how the blood BL is taken into the syringe 35a.
  • the grip portion 72a grips the flange 38a.
  • the mode of connection between the reciprocating mechanism 71a and the syringe 35a is not limited to this.
  • the grip portion 72a is connected to the actuator 73a. By transmitting the force of the actuator 73a to the flange 38a, the grip portion 72a can move the piston 32a up and down in the drawing via the rod 37a.
  • the grip portion 72b grips the flange 38b.
  • the mode of connection between the pressing mechanism 71b and the syringe 35b is not limited to this.
  • the grip portion 72b is connected to the actuator 73b. By transmitting the force of the actuator 73b to the flange 38b, the grip portion 72b can move the piston 32b downward via the rod 37b. The grip portion 72b may further move the piston 32b upward.
  • the microcircuit 20 includes an inlet 21a and an inlet 21b.
  • the microcircuit 20 further includes an outlet 22a, an outlet 22b, and an outlet 22c. Details of the structure and function of the microcircuit 20 will be described later.
  • control unit 80 causes the reciprocating mechanism 71a to pull the piston 32a via the rod 37a and the flange 38a. At this time, the control unit 80 causes the intake valve 76a to open the first conduit 11a. The control unit 80 further closes the third conduit 11c to the start valve 76b at this time.
  • the reciprocating mechanism 71a takes in blood BL from the intake port 50 via the branch 15 into the cylinder 34a of the syringe 35a.
  • a blood collection tube 51 is attached to the intake port 50.
  • the blood collection tube 51 stores the collected blood BL in advance.
  • the blood collection tube 51 may be a vacuum blood collection tube.
  • Blood BL may be undiluted whole blood.
  • a reagent may be added to the blood collection tube 51 in advance and mixed with undiluted whole blood in advance.
  • the cylinder 34a stores the diluent DL in advance.
  • the diluent DL dilutes the blood BL in the cylinder 34a.
  • a stirrer 75 is arranged in advance in the cylinder 34a. Under the control of the control unit 80, the magnet 74 of the stirring mechanism (M) may move the stirrer 75. The magnet 74 may mix the diluent DL and the blood BL.
  • FIG. 3 shows how the blood BL has been taken into the syringe 35a.
  • the control unit 80 causes the reciprocating mechanism 71a to stop the operation of pulling the piston 32a.
  • the stirring mechanism (M) moves the stirrer 75 via the magnet 74. This movement may be continued from FIG.
  • the stir bar 75 mixes the liquid in the cylinder 34a well. Diluted blood DB is obtained in the cylinder 34a by mixing the diluted solution and blood BL. Stirring also suppresses the sedimentation of blood cells in the diluted blood DB.
  • FIG. 4 shows how the diluted blood DB is poured into the fine circuit 20.
  • the control unit 80 causes the reciprocating mechanism 71a to push the piston 32a via the rod 37a and the flange 38a. At this time, the control unit 80 closes the first conduit 11a to the intake valve 76a. At this time, the control unit 80 further opens the third conduit 11c to the start valve 76b.
  • the control unit 80 further causes the pressing mechanism 71b to push the piston 32b.
  • the pressing mechanism 71b does this as the reciprocating mechanism 71a pushes the piston 32a.
  • the cylinder 34b of the syringe 35b stores the pressing liquid PL in advance. The function of the pressing liquid PL in the fine circuit 20 will be described later.
  • the reciprocating mechanism 71a continuously flows the diluted blood DB from the syringe 35a toward the microcircuit 20 via the branch 15.
  • the pressing mechanism 71b continuously flows the pressing liquid PL from the cylinder 34b toward the fine circuit 20.
  • the diluted blood DB flows from the cylinder 34a, and the pressing liquid PL flows from the cylinder 34b into the microcircuit 20 in parallel with each other.
  • the stirring mechanism (M) moves the stirrer 75 via the magnet 74. This movement may be continued from FIG.
  • the stirrer 75 stirs the diluted blood DB well. Stirring suppresses the sedimentation of blood cells in the diluted blood DB. As a result, the number of blood cells per unit volume in the diluted blood DB is made uniform.
  • the syringe 35a can send the diluted blood DB thus homogenized to the microcircuit 20. While the diluted blood DB is sent to the microcircuit 20, fluctuation of the number of blood cells per unit volume is suppressed depending on the time zone.
  • piston 32a and the piston 32b operate cooperatively under the control of the control unit 80. These feed the diluted blood DB and the pressing liquid PL into the microcircuit 20. Under the control of the control unit 80, the piston 32a, the stirrer 75, and the piston 32b operate in cooperation with each other.
  • the piston 32a, the intake valve 76a, and the start valve 76b operate cooperatively under the control of the control unit.
  • the blood BL in the blood collection tube 51 is sent to the microcircuit 20. It is preferable that the two valves cooperate further in addition to the above three elements.
  • At least one of the intake valve 76a and the start valve 76b may be replaced with a check valve. Further, these valves may be replaced with a three-way stopcock installed at the branch 15.
  • FIG. 5 shows a fluid device 30 in the form of a cartridge and a drive device 70 made in accordance with the fluid device 30.
  • the drive device 70 further includes a mounting surface 86.
  • a reciprocating mechanism 71a and a pressing mechanism 71b are arranged on the mounting surface 86.
  • the drive device 70 may further include guides 87a and 87b.
  • the guides 87a and 87b may be arranged on the mounting surface 86.
  • the fluid device 30 is composed of a flat plate cartridge.
  • the fluid device 30 is attached to the mounting surface 86 when classifying cells in blood.
  • a cover may further cover the mounting surface 86 and the fluid device 30. After finishing the classification of blood, the fluid device 30 is removed from the mounting surface 86.
  • the microcircuit 20, the intake port 50, the syringe 35a corresponding to the syringe A, the branch 15 and the syringe 35b corresponding to the syringe B are arranged in a plane in the cartridge constituting the fluid device 30. ing. The positions of these members are fixed by the case 55.
  • the fluid device 30 is attached to the drive device 70. At this time, the back surface of the cartridge-shaped fluid device 30 faces the mounting surface 86.
  • the guides 87a and 87b receive the bottom of the cartridge-like fluid device 30.
  • FIG. 5 also shows the positional relationship between each syringe and the drive device 70 after mounting.
  • the hold portion 79a grips the cylinder 34a.
  • the grip portion 72a grips the flange 38a.
  • the hold portion 79b grips the cylinder 34b.
  • the grip portion 72b grips the flange 38b.
  • the guides 87a and 87b define the mounting height of the cartridge-shaped fluid device 30, thereby aligning the heights of the members.
  • the hold portion 79a includes a support 78a.
  • the support 78a supports the lower surface of the flange 36a so that the cylinder 34a does not fall.
  • the support 78a preferably supports the lower surface of the flange 36a on both the left and right sides of the cylinder 34a. Further, the support 78a restrains the cylinder 34a so as not to move in the left-right direction in the drawing.
  • the hold portion 79a further includes a holding tool 77a.
  • the presser 77a presses the upper surface of the flange 36a to restrain the cylinder 34a so that the cylinder 34a does not protrude from the support 78a.
  • the presser 77a presses the cylinder 34a.
  • the flange 36a is sandwiched between the lower surface of the holding tool 77a and the upper surface of the support 78a.
  • the presser 77a preferably presses the upper surface of the flange 36a on both the left and right sides of the piston 32a inserted into the cylinder 34a. It is preferable that the lower surface of the holding tool 77a and the upper surface of the support 78a sandwich the flange 36a on both the left and right sides of the cylinder 34a.
  • the hold portion 79b includes a support 78b.
  • the support 78b supports the lower surface of the flange 36b so that the cylinder 34b does not fall.
  • the support 78b preferably supports the lower surface of the flange 36b on both the left and right sides of the cylinder 34b. Further, the support 78b restrains the cylinder 34b so as not to move in the left-right direction in the drawing.
  • the hold portion 79b further includes a holding tool 77b.
  • the holding tool 77b may be capable of restraining the cylinder 34b so that the cylinder 34b does not protrude from the support 78b by pressing the upper surface of the flange 36b.
  • the pressing tool 77b may be able to press the cylinder 34b.
  • the flange 36b is sandwiched between the lower surface of the holding tool 77b and the upper surface of the support 78b.
  • the presser 77b may press the upper surface of the flange 36b on both the left and right sides of the piston 32b inserted into the cylinder 34b.
  • the lower surface of the presser foot 77b and the upper surface of the support 78b may sandwich the flange 36b.
  • the magnet 74 faces the cylinder 34a.
  • the magnet 74 is arranged so as to move on the mounting surface 86.
  • the magnet 74 reciprocates in the operating area OA.
  • the operating area OA may be formed by a through hole or a recess provided on the mounting surface 86. In the figure, the operating area OA is a through hole.
  • the magnet 74 can be brought closer to the cylinder 34a by bringing the back surface of the flat plate-shaped fluid device 30 closer to the mounting surface 86. Therefore, the magnetic force of the magnet 74 is efficiently transmitted to the stirrer 75 in the cylinder 34a.
  • the case 55 has an access window 59.
  • the intake valve 76a reaches the first conduit 11a through the access window 89.
  • the intake valve 76a catches the first conduit 11a.
  • the intake valve 76a closes the inside of the first conduit 11a by crushing the first conduit 11a.
  • the intake valve 76a opens the inside of the first conduit 11a by stopping crushing the first conduit 11a.
  • the start valve 76b reaches the third conduit 11c through the access window 59.
  • the start valve 76b catches the third conduit 11c.
  • the start valve 76b closes the inside of the third conduit 11c by crushing the third conduit 11c.
  • the start valve 76b opens the inside of the third conduit 11c by stopping crushing the third conduit 11c.
  • the fluid device 30 in the form of a cartridge further includes a collection container 56 and a drainage container 57.
  • the collection vessel 56 receives cells of interest classified by the microcircuit 20, such as nucleated blood cells.
  • the drainage container 57 receives cell-free fractions and unintended cells generated by the classification operation.
  • the collection container 56 and the drainage container 57 are arranged in the case 55.
  • the collection container 56 can be taken out from the case 55.
  • the drainage container 57 may be enclosed in the case 55.
  • each component of the drive device 70 is separated from the fluid device 30.
  • the fluid circuit required for classification is mounted on the fluid device 30.
  • the drive device 70 does not include the components of the fluid device 30.
  • the drive device 70 provides the fluid device 30 with the power required for classification.
  • the fluid device 30 can be attached to the drive device 70 as a cartridge each time classification is required. Further, the fluid device 30 after classification can be removed from the drive device 70 and discarded.
  • the fluid device 30 may be disposablely replaced for each classification operation.
  • the drive device 70 may be used repeatedly. This allows cells in the blood to be classified using a clean fluid device 30 for each blood sample.
  • the stir bar 75 may also be disposable and replaced with the fluid device 30.
  • the drive device 70 is independent of each syringe and the microcircuit 20. Therefore, the blood sample is unlikely to adhere to the drive device 70. This prevents the drive device 70 from mediating contamination between different blood samples.
  • FIG. 6 shows the magnet 74 and the reciprocating mechanism 71a observed in the VI cross section shown in FIG. In this figure, it is assumed that the cartridge-shaped fluid device is already attached to the drive device. The cartridge case is omitted in the figure.
  • the actuator 73a is arranged behind the magnet 74.
  • the rear side means that the actuator 73a is located on the side opposite to the cylinder 34a with the magnet 74 in between.
  • the actuator 73a reciprocates the grip portion 72a along the longitudinal direction of the syringe 35a.
  • the actuator 73a moves the rod 37a from the back side of the magnet 74.
  • the operating area OA is located behind the cylinder 34a and faces the cylinder 34a.
  • the actuator 73a may be located behind the operating area OA.
  • the operating area OA may face the actuator 73a.
  • the grip portion 72a may straddle the operating area OA.
  • FIG. 7 shows a state in which the stirring mechanism 71c and the syringe 35a are selected and observed from the front.
  • the fluid device is already mounted on the drive unit.
  • the cartridge case is omitted in the figure.
  • the actuator 73c reciprocates the arm 72c along the longitudinal direction of the syringe 35a.
  • the arm 72c holds the magnet 74 from the side with respect to the reciprocating direction of the magnet 74.
  • the arm 72c inserts the magnet 74 from the side of the syringe 35a.
  • the magnet 74 reciprocates the stirrer 75 in the syringe 35a along the longitudinal direction of the syringe 35a.
  • the magnet 74 may be a permanent magnet or an electromagnet.
  • the magnet 74 is a permanent magnet.
  • the syringe 35a stands with the discharge port 31a at the bottom and the piston 32a at the top.
  • the syringe 35a may be upright.
  • the hold portion 79a is located above the magnet 74 and the stirrer 75.
  • the grip portion 72a is located above the hold portion 79a.
  • the magnet 74 may drop the stirrer 75 in the cylinder 34a during the reciprocating motion of the magnet 74.
  • An example is when the stirrer 75 collides with the piston 32a.
  • the stirring mechanism 71c directs the magnet 74 to the lower end of the cylinder 34a.
  • the reciprocating motion of the magnet 74 is preferably one that folds back at the lower end of the cylinder 34a. As a result, the magnet 74 can pick up the stirrer 75 that the magnet 74 has dropped again. Therefore, blood agitation continues.
  • the drive device 70 drives the fluid device 30.
  • the role of the fluid device 30 is to perform hydraulic classification by sending the diluted blood DB and the pressing liquid PL to the microcircuit 20.
  • the hydraulic description of the microcircuit 20 will be described below.
  • the microcircuit 20 is a flow path chip for separating floating cells such as blood cells.
  • the configuration of the microcircuit 20 is not limited to the following embodiment.
  • FIG. 8 shows a microcircuit 20 in a plan view.
  • the microcircuit 20 has a main flow path 23.
  • One end of the main flow path 23 is an inlet 21a.
  • the other end of the main flow path 23 is an outlet 22c.
  • the microcircuit 20 further has a subchannel 24.
  • the end of the auxiliary flow path 24 is the inlet 21b.
  • the end of the sub-flow path 24 is connected to the main flow path 23 at the merging portion 28.
  • the main flow path 23 has flow path parts 25a to 25d in order from the inlet 21a to the outlet 22c.
  • the flow path parts 25a to d are connected from the inlet 21a to the outlet 22c.
  • the inlet 21a shown in FIG. 8 is connected to the syringe 35a containing the diluted blood DB. From the syringe 35a, the diluted blood DB is sent to the inlet 21a at a predetermined flow rate. The diluted blood DB enters the flow path part 25a via the inlet 21a.
  • the diluted blood DB shown in FIG. 8 is preferably diluted before being introduced into the microcircuit 20.
  • the dilution rate can be 2 to 500 times. In this example, the dilution rate may be 50 times. Dilution is done with phosphate buffered saline.
  • the flow rate of the diluted blood DB per unit time can be 1 to 1000 ⁇ l / min. For example, it may be any of 5,10,15,20,25,30,35,40,45,50,55,60,65,70,75,80,85,90,95 and 100 ⁇ l / min.
  • the processing time may be 1 minute to 300 minutes.
  • the fine circuit 20 may be any of 5, 10, 20, 30, 40, 50, 60, 90, 120, 150, 180, 210, 240 and 270 minutes.
  • the liquid obtained by diluting, for example, 15 ml of whole blood in one classification may be processed by the fine circuit 20.
  • the microcircuit 20 shown in FIG. 8 has a subchannel 24.
  • the subchannel 24 is connected to the syringe 35b.
  • the pressing liquid PL is stored in the syringe 35b.
  • Pressing liquid PL is a liquid that does not contain floating cells.
  • Pressing liquid PL is a liquid that does not easily damage blood cells and other cells.
  • the pressing liquid PL may contain a buffer liquid.
  • the buffer may be PBS.
  • Both the branch flow paths 26a and 26b shown in FIG. 8 are flow paths that branch from the main flow path 23.
  • the branch flow path 26a and the branch flow path 26b branch from the main flow path 23 in this order from the upstream side.
  • the branch flow paths 26a and 26b are arranged on the side facing the sub flow path 24 with the main flow path 23 in between.
  • Each of the branch flow paths 26a and 26b shown in FIG. 8 has a plurality of small flow paths branching from the main flow path 23.
  • the small flow paths are lined up from the upstream to the downstream of the main flow path 23.
  • the branch flow paths 26a and 26b reach the outlets 22a and 22b, respectively.
  • the fine flow paths in the branch flow paths 26a and 26b merge before the outlets 22a and 22b, respectively.
  • the flow path part 25d reaches the outlet 22c.
  • FIG. 9 shows a microcircuit 20 in which the vicinity of the flow path part 25c is magnified.
  • the figure schematically shows the process of separating floating cells by the microcircuit 20.
  • the branch flow path 26a is represented by 10 small channels
  • the branch flow path 26b is represented by 3 small channels.
  • FIGS. 9 and 10 are taken from Patent Document 2 and partially modified so as to be suitable for the description of this embodiment. The classification mechanism is described in particular detail in Patent Document 2.
  • the diluted blood DB continuously flows from the upstream of the flow path part 25b.
  • Diluted blood DB contains a large amount of cells.
  • the flow of the pressing liquid PL is continuously contacted with the flow of the diluted blood DB from the side thereof.
  • the pressing liquid PL continuously pushes the flow of the diluted blood DB to the opposite side of the pressing liquid PL while flowing in the same direction as the diluted blood DB.
  • the pressing liquid PL continuously pushes the cells flowing through the main flow path 23 from the side of the main flow path 23.
  • the floating cells are continuously pushed toward the branch flow path 26a and the branch flow path 26b, and in the flow path part 25c, the floating cells continuously flow into these branch flow paths.
  • annuclear erythrocytes 27 continuously flow into the branch flow path 26a.
  • the anucleated erythrocytes 27 in the diluted blood DB are hydraulically classified in the channel part 25c. The classification is continuously performed on the side of the flow of the diluted blood DB that is not in contact with the flow of the pressing liquid PL.
  • the branch flow path 26a acts as a removal flow path for the anucleated erythrocytes 27.
  • the inscribed diameter of the fine flow path of the branch flow path 26a is 11 to 19 ⁇ m.
  • the inscribed diameter may be any of 12, 13, 14, 15, 16, 17, and 18 ⁇ m.
  • nucleated cells 29a-c continuously flow into the branch flow path 26b.
  • the nucleated cells 29a-c in the diluted blood DB are hydraulically classified. The classification is continuously performed on the side of the flow of the diluted blood DB that is not in contact with the flow of the pressing liquid PL.
  • Cell suspension CS is continuously obtained from the branch flow path 26b.
  • the branch flow path 26b functions as a recovery flow path for the nucleated cells 29a-c.
  • the inscribed diameter of the fine channel of the branch flow path 26b is smaller than the inscribed diameter of the fine channel of the branch flow path 26a.
  • the inscribed diameter of the fine flow path of the branch flow path 26b is 17 to 30 ⁇ m.
  • the inscribed diameter may be any of 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28 and 29 ⁇ m.
  • the diameter of nucleated cells including nucleated red blood cells is considered to be 11 to 13 ⁇ m.
  • the inscribed diameter of each of the branch flow paths 26a and 26b shown in FIG. 9 is the diameter of the inscribed circle in the orthogonal cross section of the fine flow path.
  • the cross section of each microchannel is square.
  • the cross section of the microchannel may be another polygon or a circle. The same is true for other branch channels.
  • Floating cells and plasma not taken up in the branch flow paths 26a and 26b shown in FIG. 9 continuously pass through the flow path part 25d as a flow-through FT. After that, it reaches the exit 22c shown in FIG.
  • aggregated blood cells are included in the flow-through FT.
  • FIG. 10 shows the details of the microcircuit while focusing on the flow path part 25c.
  • the value of the inscribed diameter of the microchannel is not equal to the maximum value of the diameter of the suspended cells to be classified. Therefore, the hydraulic classification according to this embodiment is different from simple filtration. The hydraulic classification according to this embodiment will be described below.
  • FIG. 10 shows the flow path part 25c. Further, a branch flow path 26a is represented. For the sake of simplicity, only one fine flow path constitutes the branch flow path 26a. In the description of FIG. 10, the fine flow path constituting the branch flow path 26a is simply referred to as a branch flow path 26a.
  • the liquid flow LF is continuously introduced into the flow path part 25c.
  • the liquid flow LF contains the above-mentioned diluted blood DB and the pressing liquid PL. These liquids are partially mixed in the liquid flow LF.
  • Nucleated cells 29a as large cells and annuclear erythrocytes 27 as small cells are suspended in the fluid flow LF. In the figure, the nucleated cell 29a is drawn on behalf of other nucleated cells.
  • the portion of the liquid flow LF introduced into the branch flow path 26a is referred to as the liquid flow LE.
  • the portion of the liquid flow LF that is not introduced into the branch flow path 26a and flows downstream is referred to as the liquid flow LG.
  • the flow rate of the liquid flow LE is smaller than a certain value.
  • the flow rate is proportional to the cross section of the liquid flow LE.
  • the liquid flow LE is located on the inner wall of the flow path part 25c on the branch flow path 26a side.
  • the flow rate of the liquid flow LE is also proportional to the flow rate of the liquid flow LE in the branch flow path 26a.
  • annuclear erythrocytes 27 flowing in the liquid flow LE are introduced into the branch flow path 26a.
  • more than half of the volume of the nucleated cell 29a belongs to the liquid flow LG side. Nucleated cells 29a make only partial contact with the fluid LE. Therefore, the nucleated cells 29a are not introduced into the branch flow path 26a. At this time, the diameter of the nucleated cell 29a may be smaller than the inscribed diameter of the branch flow path 26a. If the flow rate of the liquid flow LE becomes large, the cross section of the liquid flow LE becomes large. In this case, it is conceivable that the nucleated cells 29a are swallowed by the liquid flow LE and are guided to the branch flow path 26a.
  • the nucleated cells 29a are carried further downstream on the liquid flow LG. As described above, a fluid having a certain size or larger and containing no suspended cells can be recovered from the branch flow path 26a. Nucleated cells 29a and other nucleated cells are also classified downstream.
  • FIG. 11 shows a flow chart of the operation of the microcircuit (F) for the work of classifying cells in blood. Further, a flow chart of the operation of the reciprocating mechanism (A), the pressing mechanism (B) and the stirring mechanism (M) for operating the fine circuit (F) is shown.
  • FIG. 12 shows the state of the drive device 70 and the fluid device 30 prior to blood uptake.
  • blood BL is collected in the blood collection tube 51 as shown in FIG. 12 as a preliminary preparation.
  • the blood BL is whole blood.
  • the blood collection tube 51 storing the blood BL is attached to the inlet 50. Further, by injecting the diluted solution DL into the syringe 35a in advance, this is placed in the syringe 35a. Further, a stirrer 75 is also arranged in the syringe 35a. Further, by injecting the pressing liquid PL into the syringe 35b in advance, this is placed in the syringe 35b. As soon as the preparations are completed, the classification work will start.
  • step S91 of FIG. 11 the inside of the microcircuit (F) and the surrounding conduit is immersed in a liquid.
  • the syringe 35a supplies the diluent DL into the second conduit 11b, the branch 15, the third conduit 11c, and the microcircuit 20.
  • the intake valve 76a is closed and the start valve 76b is opened.
  • step S91 shown in FIG. 11 the stirring mechanism (M) is stopped.
  • step S92 of FIG. 11 the immersion of the fine circuit (F) is stopped.
  • the liquid feeding to the fine circuit (F) is stopped.
  • the fine circuit (F) also pauses.
  • the stirring mechanism (M) may also be paused in step S92.
  • step S93 of FIG. 11 the reciprocating mechanism (A) pulls the piston.
  • the intake valve 76a is opened and the start valve 76b is closed.
  • the valve By operating the valve, a route from the blood collection tube 51 to the syringe 35a via the intake 50 and the branch 15 is formed.
  • the piston 32a By pulling the piston 32a, the blood BL is taken into the syringe 35a along this route. That is, as the volume of the syringe 35a is expanded, a predetermined amount of blood BL is taken into the syringe 35a from the intake port 50 via the branch 15.
  • the stirring mechanism (M) moves the stirrer 75 from the outside of the syringe 35a to stir the diluent DL and the blood so that the suspended cells in the blood do not settle in the syringe 35a. Further, the mixing of the blood BL before dilution and the diluted solution DL may be sufficient by stirring with the stirrer 75.
  • step S93 shown in FIG. 11 the microcircuit (F) and the pressing mechanism (B) are stopped.
  • step S94 shown in FIG. 11 the drawing of blood by the reciprocating mechanism (A) is stopped.
  • the fine circuit 20 corresponding to the fine circuit (F) and the pressing mechanism 71b corresponding to the pressing mechanism (B) are stationary.
  • the pressing mechanism 71b may continue pressing without pausing.
  • the stirring mechanism (M) continues stirring the diluted blood DB.
  • step S95 shown in FIG. 11 blood cells in blood are classified by a microcircuit (F).
  • the start valve 76b is opened and released as shown in FIG. Further, the intake valve 76a is closed and all the pressure is directed to the fine circuit 20. As a result, a route from the syringe 35a to the microcircuit 20 via the branch 15 is formed.
  • step S95 shown in FIG. 11 the reciprocating mechanism (A) and the pressing mechanism (B) press against each syringe.
  • the reciprocating mechanism 71a pushes the piston 32a. Pushing the piston 32a may be simultaneous with the formation of a route by each valve.
  • the syringe 35a continuously flows the diluted blood DB into the microcircuit 20 by the pressure generated by the piston 32a.
  • the pressing mechanism 71b further pushes the piston 32b.
  • the syringe 35b continuously flows the pressing liquid PL into the microcircuit 20 by the pressure generated by the piston 32b.
  • the microcircuit 20 continuously classifies suspended cells in the diluted blood DB while using the pressing solution PL.
  • blood cells or floating cells that are not blood cells are hydraulically classified in the microcircuit 20. Details of the hydraulic classification have been described with reference to FIGS. 8-10.
  • the microcircuit 20 can separate and concentrate PBMC (peripheral blood mononuclear cells). Further, the classified cells may be taken out from the discharge port on the lower surface of the microcircuit 20.
  • PBMC peripheral blood mononuclear cells
  • step S95 After processing a predetermined amount of diluted blood DB in step S95 shown in FIG. 11, the pressing by the reciprocating mechanism (A) and the pressing mechanism (B) is stopped in step S96. Classification in the fine circuit (F) is also suspended. Further, the stirring of the diluted blood by the stirring mechanism (M) may be stopped in accordance with these. As described above, all the processes are completed, and the classification work is completed in step S99. The classification work may be resumed if necessary.
  • FIG. 13 schematically shows the control unit 80.
  • the control unit 80 includes a sequencer 81.
  • the sequencer 81 is a PLC (programmable logic controller).
  • the sequencer 81 includes a non-volatile memory 82.
  • the memory 82 stores the program of the cooperative operation of each mechanism shown in FIG.
  • the sequencer 81 automatically executes the cooperative operation according to the time schedule derived from the program stored in the memory 82.
  • the control unit 80 further includes motor drivers 83a, 83b and 83c.
  • the motor drivers 83a, 83b and 83c are connected to the sequencer 81, respectively.
  • the motor drivers 83a, 83b and 83c are connected to the actuators 73a, 73b and 73c, respectively.
  • the actuators 73a, 73b and 73c are components of the reciprocating mechanism (A), the pressing mechanism (B) and the stirring mechanism (M), respectively.
  • the motor drivers 83a, 83b and 83c operate the reciprocating mechanism (A), the pressing mechanism (B) and the stirring mechanism (M), respectively.
  • control unit 80 is further connected to the intake valve 76a and the start valve 76b. Both the intake valve 76a and the start valve 76b may be a pinch valve operated by a solenoid.
  • the control unit 80 operates the intake valve 76a and the start valve 76b in accordance with the operation of the reciprocating mechanism (A).
  • the drive device 70 further includes a power supply 85.
  • the power supply 85 is connected to the sequencer 81 and the motor drivers 83a, 83b and 83c, respectively.
  • the power supply 85 supplies to the control unit 80.
  • the control unit 80 operates with the electric power of the power source 85.
  • the power supply 85 supplies electric power to the actuators 73a, 73b and 73c via the motor drivers 83a, 83b and 83c, respectively.
  • Each motor driver and actuator is powered by power 85.
  • the power supply 85 may be connected to the intake valve 76a and the start valve 76b.
  • the power supply 85 may supply the power required to operate the intake valve 76a and the start valve 76b. All the functions of the control unit 80 may be completed in the drive device 70. Some functions may be outside the drive 70.
  • FIG. 14 is a front view showing a modified example of the drive device.
  • the drive device 40 makes the microcircuit 20, the reservoir 45, the syringe 35a, and the syringe 35b cooperate with each other.
  • the reservoir 45 stores preclassified blood. These elements are not integrated as the fluid device 30 shown in FIG. 1 or the like or as a cartridge thereof.
  • the drive device 40 shown in FIG. 14 does not use the three-way branch 15 shown in FIG. 1 or the like or the blood intake 50 connected to the branch 15. Further, in order to arrange the traveling direction of blood in the branch 15 shown in FIG. 1 and the like, opening and closing of the valve 76a and the valve 76b is not controlled. The drive device 40 is otherwise common to the drive device 70 shown in FIG. 1 and the like.
  • the drive device 40 includes a control unit 48.
  • the control unit 48 includes a reciprocating mechanism 71a, a mounting mechanism 46, a pressing mechanism 71b, and a stirring mechanism 71c.
  • the drive device 40 includes a control unit 48 that controls these cooperative operations.
  • the control unit 48 differs from the control unit 80 in that it does not control the valves 76a and 76b shown in FIG. 1 and the like, but instead controls the mounting mechanism 46 shown in FIG.
  • the control unit 48 is otherwise common to the control unit 80.
  • the reciprocating mechanism 71a takes in blood from the reservoir 45 toward the inside of the syringe 35a by pulling the piston 32a of the syringe 35a.
  • the syringe 35a takes in blood through the discharge port 31a.
  • the mounting mechanism 46 transports the syringe 35a from the location of the reservoir 45 to the location of the microcircuit 20.
  • the mounting mechanism 46 mounts the discharge port 31a of the syringe 35a at the inlet 21a of the microcircuit 20.
  • an adapter 43a is attached to the inlet 21a of the microcircuit 20.
  • the mounting mechanism 46 inserts the discharge port 31a into the adapter 43a.
  • the inlet 21a of the microcircuit 20 and the discharge port 31a of the syringe 35a are coupled via an adapter 43a.
  • the adapter 43a may be freely attached to and detached from the inlet 21a of the discharge port 31a. After mounting the discharge port 31a, the adapter 43a may not be able to remove the discharge port 31a from the inlet 21a.
  • the discharge port 31b of the syringe 35b is connected to the inlet 21b on the upper surface of the microcircuit 20.
  • An adapter 43b is attached to the inlet 21b of the microcircuit 20.
  • the inlet 21a of the microcircuit 20 and the discharge port 31a of the syringe 35a are coupled via an adapter 43a.
  • the stirrer arranged in the syringe 35a is moved by the stirring mechanism 71c from the outside of the syringe 35a to mix and stir the blood in the syringe 35a and the diluent to obtain the diluted blood DB. While the diluted blood DB is stored in the syringe 35a, the magnet 74 moves the stirrer 75 from the outside of the syringe 35a to stir the blood in the syringe 35a.
  • the reciprocating mechanism 71a continuously flows the diluted blood DB from the syringe 35a toward the microcircuit 20 via the discharge port 31a and the inlet 21a by pushing the piston 32a.
  • the pressing mechanism 71b continuously flows the pressing liquid PL from the syringe 35b toward the microcircuit 20 by pushing the piston B of the syringe 35b in accordance with the reciprocating mechanism 71a pushing the piston 32a.
  • the cells in the blood stored in the reservoir 45 shown in FIG. 14 can be classified by the microcircuit 20. Details of classification in the fine circuit 20 are performed as shown in FIGS. 8 to 10.

Abstract

Un dispositif de fluide (30) comprend un microcircuit (20), une ouverture d'admission (50) pour le sang, une seringue A (35a) qui accumule le sang, et une seringue B (35b) pour accumuler un liquide de pression. Un appareil d'entraînement (70) comprend un mécanisme de va-et-vient (71a), un mécanisme de pression (71b), un mécanisme d'agitation (71c), et une unité de commande (80). Le mécanisme de va-et-vient (71a) tire un piston A (32a) pour ainsi prélever du sang dans la seringue A (35a). Le mécanisme de va-et-vient (71a) pousse en outre le piston A (32a) pour ainsi amener le sang à s'écouler en continu dans le microcircuit (20). Le mécanisme de pression (71b) pousse un piston B (32b) pour amener ainsi un liquide de pression PL à s'écouler en continu dans le microcircuit (20). Un aimant (74) du mécanisme d'agitation (71c) amène un élément d'agitation (75) à se déplacer de l'extérieur de la seringue A (35a).
PCT/JP2020/013180 2019-03-29 2020-03-25 Appareil pour commander une combinaison d'un microcircuit et d'une seringue WO2020203510A1 (fr)

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JP2019066212 2019-03-29
JP2019-066212 2019-03-29
JP2020-020665 2020-02-10
JP2020020665A JP2020162585A (ja) 2019-03-29 2020-02-10 微細回路とシリンジとの組み合わせに対する駆動装置

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6274369A (ja) * 1985-09-30 1987-04-06 日機装株式会社 シリンジポンプ
JPH11322618A (ja) * 1998-05-13 1999-11-24 Asahi Medical Co Ltd 有核細胞分離回収方法及び有核細胞含有液
JP2011013208A (ja) * 2009-06-05 2011-01-20 Advance Co Ltd 生物学的操作システム及び工業的操作システム
JP2011088869A (ja) * 2009-10-26 2011-05-06 Osaka Univ 白血球タンパク質の回収方法および回収装置
JP2018102242A (ja) * 2016-12-27 2018-07-05 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 胎児細胞由来染色体ｄｎａの取得方法
WO2020036115A1 (fr) * 2018-08-14 2020-02-20 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ Dispositif fluidique

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6274369A (ja) * 1985-09-30 1987-04-06 日機装株式会社 シリンジポンプ
JPH11322618A (ja) * 1998-05-13 1999-11-24 Asahi Medical Co Ltd 有核細胞分離回収方法及び有核細胞含有液
JP2011013208A (ja) * 2009-06-05 2011-01-20 Advance Co Ltd 生物学的操作システム及び工業的操作システム
JP2011088869A (ja) * 2009-10-26 2011-05-06 Osaka Univ 白血球タンパク質の回収方法および回収装置
JP2018102242A (ja) * 2016-12-27 2018-07-05 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 胎児細胞由来染色体ｄｎａの取得方法
WO2020036115A1 (fr) * 2018-08-14 2020-02-20 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ Dispositif fluidique

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