WO2020135128A1

WO2020135128A1 - Sample mixing apparatus, sample analysis system, and sample mixing method

Info

Publication number: WO2020135128A1
Application number: PCT/CN2019/125692
Authority: WO
Inventors: 胡力坚; 刘元幸
Original assignee: 深圳迈瑞生物医疗电子股份有限公司
Priority date: 2018-12-28
Filing date: 2019-12-16
Publication date: 2020-07-02
Also published as: CN113167805A; WO2020133182A1

Abstract

A sample mixing apparatus (1), a sample mixing system and a blood sample analysis system comprising the sample mixing apparatus (1), a corresponding sample mixing method, a control apparatus for a sample analysis system, and a computer readable storage medium. The sample mixing apparatus (1) comprises a sample container base (12) where a sample container (2) is accommodated, and a drive mechanism (13) for driving the sample container base (12) to rotate around a rotation axis thereof. The sample container base (12) is configured, when the sample container (2) is accommodated in the sample container base (12), that an acute included angle (α) is formed between a central axis of the sample container (2) and the rotation shaft of the sample container base (12).

Description

Sample mixing device, sample analysis system and sample mixing method

Technical field

The invention relates to the field of blood sample analysis, in particular to a sample mixing device, a sample analysis system, and a sample mixing method for mixing drawn blood samples, especially a trace amount of blood samples.

Background technique

Blood sample measurement requires a certain amount of sample from the patient. Blood collection methods are generally divided into collecting venous blood and collecting peripheral blood. For neonates, infants, intensive care patients and other patients who are not suitable for venous blood collection, peripheral blood is often collected.

During blood collection, in order to prevent blood clotting, blood collection tubes containing anticoagulants are usually used. The blood is composed of blood cells and plasma. Due to the different specific gravity of blood cells and plasma, the anticoagulated blood will stratify after standing for a period of time, so the blood sample needs to be thoroughly mixed before measurement, otherwise the measurement results will have a large deviation .

At present, when the analyzer on the market measures peripheral blood, it is usually necessary to mix the peripheral blood sample in the blood collection tube with a vortex oscillator in advance, or flick the finger, and then put it into the analyzer for measurement. However, this method of mixing greatly limits the number of batch measurements of peripheral blood at one time, and increases the burden of manual operation, which is extremely inconvenient.

In addition, in the prior art, the inverse mixing method is also used to mix the peripheral blood samples in the blood collection tube. However, the characteristic of collecting the peripheral blood is that the blood collection volume is small (usually ≤150uL), so the blood sample has poor fluidity and the blood collection tube is inverted Peripheral blood often adheres to the blood collection tube cap, the bottom of the blood collection tube or the wall of the tube, and the reverse mixing technique will cause blood sample loss and adversely affect the measurement, and it is still difficult to effectively solve the problem of peripheral blood mixing.

Summary of the invention

Based on the technical problems in the sample mixing technology in the prior art and the urgent need for fully automated measurement of peripheral blood in the market, the first aspect of the present invention provides a sample mixing device including a container for containing a sample container A sample container seat and a driving mechanism for driving the sample container seat to rotate about its axis of rotation, wherein the sample container seat is configured such that when the sample container is fixedly received in the sample container seat, The central axis of the sample container and the rotation axis of the sample container seat form an acute angle.

As an implementation, the sample container holder may be configured such that when the sample container is fixedly received in the sample container holder, the central axis of the sample container and the rotation axis of the sample container holder are The intersection of the projection lines in the vertical plane is located above the bottom of the sample container containing the sample cavity.

As an implementation, the sample container holder may be configured such that when the sample container is fixedly received in the sample container holder, the central axis of the sample container and the axis of rotation of the sample container holder sample The container seats are located in the same plane, the central axis of the sample container intersects the rotation axis of the sample container seat, and the intersection point is located above the bottom of the cavity of the sample container.

As an alternative implementation, the sample container holder may be configured such that when the sample container is fixedly received in the sample container holder, the central axis of the sample container and the sample container holder The rotation axes are located in different planes, and the intersection of the central axis of the sample container and the projection line of the rotation axis of the sample container seat in the vertical plane is above the bottom of the cavity of the sample container.

As an implementation manner, the sample container holder may have a sample container accommodating cavity capable of fixedly accommodating the sample container, the sample container accommodating cavity may be configured as an accommodating cavity in the form of a hole, or the sample container The accommodating cavity is configured as a cavity surrounded by a plurality of cylinders, and the sample container can be fixedly accommodated in the cavity. Wherein, the sample container accommodating cavity may be configured such that when the sample container is fixedly accommodated in the sample container accommodating cavity, the central axis of the sample container and the rotation axis of the sample container seat are formed The included angle of the acute angle, preferably the intersection of the projection axis of the sample container center axis and the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container containing cavity of the sample container.

As an implementation manner, an abutting portion may be provided in the sample container accommodating cavity, and the abutting portion is configured such that when the sample container is accommodated in the sample container accommodating cavity, the sample The container abuts on the abutting portion, so that the central axis of the sample container and the axis of rotation of the sample container seat form an acute angle, preferably the central axis of the sample container and the sample container seat rotate The intersection point of the projection line of the axis in the vertical plane is located above the bottom of the sample container containing the sample chamber. In addition, the abutment portion may be integrally formed at the bottom of the sample container accommodating cavity of the sample container holder, or the abutment portion may be detachably fixed to the sample container container of the sample container holder Place the bottom of the cavity.

As an alternative implementation, the sample container accommodating cavity is configured such that the central axis forms an acute angle with the rotation axis of the sample container seat, so that when the sample container is fixedly received in the sample When the container accommodating chamber is formed, the central axis of the sample container and the rotation axis of the sample container seat form an acute angle, preferably the central axis of the sample container and the rotation axis of the sample container seat are vertical The intersection of the projection lines in the plane is located above the bottom of the sample container-containing cavity of the sample container.

As an implementation manner, the included angle between the central axis of the sample container and the axis of rotation of the sample container seat may be less than or equal to about 45°, preferably in the range of about 2° to about 15°.

As an implementation manner, the driving mechanism may have a driving shaft, and the sample container seat is directly fixed on the driving shaft or indirectly through a transmission mechanism, such as a belt, a timing belt, a gear set component, and the driving shaft Turn the connection.

As an alternative implementation, the driving mechanism may rotate the sample container seat by driving a rotating wheel with an elastic outer peripheral washer, and the outer peripheral washer contacts the outer periphery of the sample container seat to provide frictional force.

As an implementation manner, the sample container seat may include a rotating shaft fixing hole connected to the driving rotating shaft of the driving mechanism, and the driving rotating shaft of the driving mechanism is inserted into the rotating shaft fixing hole and fixed to the rotating shaft fixing hole Connected so that the sample container seat rotates as the drive shaft of the drive mechanism rotates.

As an implementation manner, the rotating shaft fixing hole may be coaxially arranged with the sample container accommodating cavity; or the rotating shaft fixing hole may also be eccentrically arranged with the sample container accommodating cavity, with an eccentricity of 0 mm to about 5 mm , Preferably it is about 1 mm to about 2 mm.

As an implementation manner, the driving mechanism may be configured as a motor, such as a stepper motor, or a DC motor, or a servo motor.

As an implementation manner, the sample mixing device may further include a sensor for detecting the rotation state of the sample container holder. The sensor may be, for example, a through-beam photoelectric sensor, a reflection-type photoelectric sensor, a Hall sensor, or a capacitance sensor, and is preferably a through-beam photoelectric sensor.

As an implementation manner, the sample mixing device may be provided with a sensing portion and a notch in the sensing area of the sensor. When the sample mixing seat is driven by the driving mechanism to rotate, the sensing portion and the notch Enter the sensing area of the sensor alternately to generate the output pulse signal of the sensor for detecting the rotation state of the sample container seat.

A second aspect of the present invention provides a sample mixing system including a sample container for containing a sample and a sample mixing device for mixing the sample in the sample container, wherein the sample is mixed The device is constructed according to one of the aforementioned sample mixing devices.

As an implementation manner, the sample container is configured as a micro blood collection tube for containing peripheral blood, and/or the sample container is configured as a closed container or an open container.

A third aspect of the present invention provides a sample analysis system, including:

The sample mixing device, especially the sample mixing device described above, is configured to mix the sample in the sample container and includes a sample container holder for accommodating the sample container and a drive for driving the sample container holder around A driving mechanism whose rotation axis rotates, wherein the sample container seat is configured such that when the sample container is accommodated in the sample container seat, the central axis of the sample container and the rotation axis of the sample container seat Forming an acute angle;

The control device is configured to be communicatively connected with the driving mechanism to control the driving mechanism to drive the sample container holder to rotate around its rotation axis.

As an implementation, the control device may be configured to control the driving mechanism in the following manner:

Causing the drive mechanism to drive the sample container holder to rotate in the same direction about its axis of rotation, preferably the drive mechanism to drive the sample container holder to periodically rotate in the same direction about its axis of rotation; or

The driving mechanism drives the sample container seat to rotate alternately in a first direction and a second direction opposite to the first direction about its axis of rotation, preferably, the driving mechanism drives the sample container seat around it After the rotation axis rotates in the first direction for a predetermined period of time, the drive mechanism is stopped, and after the sample returns to the bottom of the cavity of the sample container, the drive mechanism is turned on to drive the sample container seat around its rotation axis Turn in a second direction opposite to the first direction.

As an implementation manner, the sample mixing device may further include a sensor for detecting the rotation state of the sample container holder, the sensor can be communicatively connected with the control device, so as to connect the detected sample container holder The rotation state of is transmitted to the control device, the control device can adjust the driving parameters of the driving mechanism according to the rotation state of the sample container holder or can judge the sample container according to the rotation state of the sample container holder Whether the seat or the drive mechanism is malfunctioning.

As an implementation manner, the sample analysis system may further include:

A first conveying device configured to be communicatively connected to the control device and used to place the sample container into the sample container seat under the control of the control device;

The second transport device is configured to be communicatively connected to the control device and used to remove the sample container from the sample container seat under the control of the control device.

The first conveying device and the second conveying device may be configured as the same device or different devices.

The fourth aspect of the present invention provides a sample mixing method, including:

The sample container is fixedly placed in the rotatable sample container seat, so that the central axis of the sample container and the rotation axis of the sample container seat form an acute angle, preferably the central axis of the sample container and the The intersection point of the projection line of the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container containing the sample container;

The sample container holder is driven by the driving mechanism to rotate the driving mechanism around its axis of rotation to perform the sample mixing operation.

As an implementation manner, the sample container can be fixedly placed in the rotatable sample container seat, so that the included angle between the central axis of the sample container and the axis of rotation of the sample container seat is less than or equal to about 45 °, preferably in the range of about 2° to about 15°.

As an implementation manner, the step of driving the sample container base to rotate about its rotation axis by the driving mechanism to perform the sample mixing operation may include:

The sample container holder is driven to rotate in the same direction around its axis of rotation by the drive mechanism, preferably the sample container holder is driven by the drive mechanism to periodically rotate in the same direction about its axis of rotation; or

The sample container holder is driven by the driving mechanism to rotate alternately in a first direction and a second direction opposite to the first direction around its axis of rotation. Preferably, the sample container holder is driven by the driving mechanism about its axis of rotation along the first After rotating in one direction for a predetermined period of time, stop the driving mechanism and wait for the sample to flow back to the bottom of the cavity of the sample container before turning on the driving mechanism to drive the sample container holder around its axis of rotation in the reverse direction The second direction rotates in the first direction.

As an implementation manner, the method may further include:

The sensor detects the rotation state of the sample container holder;

Adjust the drive parameters of the drive mechanism, such as the speed, according to the rotation state; and/or

According to the rotation state, it is determined whether the sample container holder or the driving mechanism is malfunctioning.

In addition, the method further includes stopping the driving mechanism after the sample mixing operation is completed, and after the sample returns to the bottom of the cavity of the sample container, taking out the sample container or directly performing a sample on the sample container Suck samples.

A fifth aspect of the present invention provides a control device for a sample analysis system, including:

At least one processor; and

The memory stores instructions executable by the at least one processor, and when executed by the at least one processor, the instructions cause the sample analysis system to perform the steps of the foregoing sample mixing method.

A sixth aspect of the present invention provides a computer-readable storage medium that stores computer-executable instructions, which when executed by at least one processor of a sample analysis system, causes the sample analysis system to execute the foregoing Each step of the sample mixing method.

According to the present invention, by tilting the central axis of the sample container contained in the sample container holder relative to the rotation axis of the sample container holder, the sample container holder can better meet the requirements of sample mixing when the sample container is driven to rotate In addition, the liquid level of the sample to be mixed can be controlled by adjusting the inclination angle of the central axis of the sample container and the rotation axis of the sample container holder to prevent the sample to be mixed from overflowing the sample container. That is to say, by reasonably adjusting the rotation speed and the inclination angle, a dynamic balance can be finally achieved, that is, the sample to be mixed can not only make a circular rotation at a large angular velocity around the rotation axis, but also not cause the liquid surface to climb up. Too high, which can prevent the sample from overflowing the sample container, and by suppressing the height of the liquid surface when the sample is rotated, the residual amount of the sample hanging on the inner wall of the container cavity of the sample container is reduced, thereby reducing the sample loss.

BRIEF DESCRIPTION

1 is a perspective view of a sample mixing device according to an embodiment of the present invention;

2 is a perspective cross-sectional view of a sample container according to an embodiment of the present invention;

3 is a perspective view of a sample container holder of a sample mixing device according to an embodiment of the present invention;

4 is a schematic diagram of a sensor output signal of a sample mixing device according to an embodiment of the present invention;

5 is a cross-sectional view of a first embodiment of a sample container holder of a sample mixing device of the present invention;

6 is a cross-sectional view of the sample container holder in FIG. 5 when it is at rest, together with the sample container accommodated in its sample container accommodating cavity, where the sample container is contained in the sample container;

7 is a cross-sectional view of the sample container holder in FIG. 5 when it is rotated together with the sample container accommodated in its sample container accommodating cavity, where the sample container is contained in the sample container;

8 is a perspective view of a second embodiment of the sample container holder of the sample mixing device of the present invention;

9 is a schematic diagram of the force analysis of the sample in the sample container when the sample container holder according to the embodiment of the present invention rotates;

10 to 12 are schematic diagrams of the intersection of the central axis of the sample container and the axis of rotation of the sample container holder according to an embodiment of the present invention;

13 is a cross-sectional view of a third embodiment of the sample container holder of the sample mixing device of the present invention;

14 is a cross-sectional view of the sample container holder of FIG. 13 together with the sample container accommodated in its sample container accommodating cavity when it is at rest, in which a sample is contained in the sample container;

15 is a cross-sectional view of a fourth embodiment of the sample container holder of the sample mixing device of the present invention;

FIG. 16 is a cross-sectional view of the sample container holder of FIG. 15 together with a sample container accommodated in its sample container accommodating cavity when it is at rest, in which a sample is contained in the sample container;

17 is a schematic structural diagram of an embodiment of a sample mixing device of the present invention;

18 is a schematic structural diagram of an embodiment of a sample analysis system of the present invention;

19 is a schematic flowchart of a first embodiment of a sample mixing method of the present invention;

20 is a schematic flowchart of a first mixing method of the sample mixing method of the present invention;

21 is a schematic flowchart of a second mixing method of the sample mixing method of the present invention;

22 is a schematic flowchart of a third mixing method of the sample mixing method of the present invention;

23 is a schematic flowchart of a second embodiment of a sample mixing method of the present invention.

24 is a schematic structural diagram of a control device according to an embodiment of the present invention.

detailed description

In order to understand the features and technical contents of the embodiments of the present invention in more detail, the following describes the implementation of the embodiments of the present invention in detail with reference to the accompanying drawings. The accompanying drawings are for reference only and are not intended to limit the embodiments of the present invention.

FIG. 1 is a perspective view of a sample mixing device 1 according to an embodiment of the present invention. As shown in FIG. 1, the sample mixing device 1 of the embodiment of the present invention includes a sample container holder 12 for fixedly containing a sample container (not shown) and a drive for driving the sample container holder 12 to rotate about its rotation axis Mechanism 13, wherein the sample container holder 12 is configured such that when the sample container is fixedly received in the sample container holder 12, the central axis of the sample container and the axis of rotation of the sample container holder 12 An acute angle is formed, which is described in further detail below.

In the embodiment of the present application, the sample container in the sample container fixing base 12 not only has the revolving motion around the rotation axis but also the self-rotating motion driven by the driving mechanism 13, and can achieve a better mixing effect.

Especially for the sample container 2 shown in FIG. 2, the sample container 2 has a tube body 21 and an inner cavity 211 and is suitable for containing a micro blood sample. The bottom 212 of the inner cavity 211 is higher than the bottom end of the tube body 21. The rotation of the sample container 2 itself is particularly important for the mixing of the sample at a specific distance from the bottom of the inner cavity 212 to the tube 21 at a specific distance. Better mixing effect, so that the mixing device 1 has better adaptability to sample containers of different shapes.

It should be noted here that when the sample container is fixedly accommodated in the sample container holder, the axis of rotation of the sample container holder is the axis of rotation of the sample container or the sample contained in the sample container.

In some embodiments, the driving mechanism 13 may be configured as a motor to drive the sample container holder 12 to rotate clockwise and/or counterclockwise. The motor may be, for example, a stepper motor, a DC motor, a servo motor, or other device that can provide rotational power. The drive mechanism 13 configured as a stepper motor is a preferred embodiment of the present invention.

In some embodiments, the sample container holder 12 is rotatably connected with a driving mechanism 13, especially a motor, so that the sample container holder 12 rotates about the axis of rotation together with the sample container fixedly accommodated in the sample container holder 12. The sample container holder 12 can be directly fixed on the driving shaft of the motor 13, or can be indirectly connected to the driving shaft of the motor 13 through a transmission mechanism, such as a belt, a timing belt, a gear set component, and the like. In an alternative embodiment, the driving mechanism 13 may rotate the sample container seat 12 by driving a runner with an elastic outer peripheral washer to provide frictional force through the outer peripheral washer contacting the outer periphery of the sample container seat 12. Among them, directly fixing the sample container base 12 on the rotating shaft of the motor 13 is a preferred embodiment of the present invention, because this connection method has a more compact structure, uses fewer components, and has advantages of miniaturization and low cost.

Further, the driving mechanism 13 may be communicatively connected with a control device (not shown), which is configured to control the startup, shutdown, and rotation speed of the driving mechanism 13.

In addition, in the embodiment shown in FIG. 1, the sample mixing device 1 further includes a sensor 14 configured to detect the rotation state of the sample container holder 12, for example, to detect whether the sample container holder 12 rotates and its rotation speed, etc. . In some embodiments, the sensor 14 may be an epi-photoelectric sensor, a reflective photoelectric sensor, a Hall sensor, a capacitive sensor, or the like. Wherein, the sensor 14 is a through-beam photoelectric sensor is a preferred embodiment of the present invention.

Further, the sensor 14 can also be communicatively connected with the above-mentioned control device, so as to transmit the rotation state of the sample container holder 12 to the control device. The control device can adjust the rotation speed of the driving mechanism 13 according to the rotation state of the sample container holder 12 or Further, according to the rotation state of the sample container holder 12, it is determined whether the sample mixing device 1 is malfunctioning, for example, whether the sample container holder is stuck. For example, when the sensor 14 detects that the rotation speed of the sample container base is zero during the mixing operation, the control device may determine that the sample mixing device 1 has failed, for example, there may be a failure of the control device to open the driving mechanism 13 or the sample container base 12 Failure condition that the connection to the drive mechanism 13 is broken or the sample container holder is stuck. Alternatively, when the rotation speed of the sample container seat detected by the sensor 14 is less than the predetermined rotation speed, the control device may also determine that the sample mixing device 1 has failed, for example, the connection between the sample container seat 12 and the drive mechanism 13 is loose and causes slippage, thereby The speed drops.

In addition, in the embodiment shown in FIG. 1, the sample mixing device 1 further includes a bracket 11 for fixing the driving mechanism 13 and the sensor 14.

FIG. 3 shows a perspective view of the sample container holder 12 of the sample mixing device 1 according to the embodiment of the present invention. As shown in FIG. 3, the sample container holder 12 has a sample container accommodating chamber 121 that can fixedly receive the sample container. In this embodiment, the sample container accommodating cavity 121 is provided above the sample container seat. In addition, a sensing portion 124 and a notch 125 are provided below the sample container holder 12. When the driving mechanism 13 drives the sample container holder 12 to rotate, the sensing portion 124 and the notch 125 will alternately pass through the sensing area of the sensor 14, that is, the sensing area of the sensor 14 will alternately switch between the blocked state and the unblocked state. The sensor 14 The pulse signal as shown in (a) or (b) of FIG. 4 is output, and the pulse signal output by the sensor 14 can be used to determine whether the sample container holder 12 rotates and the rotation speed. For example, by calculating the number of pulse signals output by the sensor 14, the number of rotations of the sample container holder 12 can be determined, so that the control device connected to the sensor 14 can determine whether the number of rotations is as expected. By calculating the signal period T of the pulse signal shown in FIG. 4, the rotation speed of the sample container holder 12 can be calculated, so that the control device connected to the sensor 14 can determine whether the rotation speed is in line with expectations.

Next, the first embodiment of the sample container holder 12 of the present invention will be described with reference to FIGS. 5 to 7, FIG. 5 is a cross-sectional view of the first embodiment of the sample container holder 12 of the sample mixing device 1 of the present invention, and FIG. 6 is FIG. 5 The sample container holder 12 in the cross-sectional view of the sample container 2 contained in the sample container accommodating cavity 121 when it is at rest, wherein the sample container 2 contains the sample 3, and FIG. 7 is the sample container holder in FIG. 5 12 is a cross-sectional view of the sample container 2 accommodated in the sample container accommodating cavity 121 during rotation, in which the sample 3 is accommodated.

As shown in FIG. 5, the sample container holder 12 has a sample container accommodating cavity 121, and the diameter at the entrance of the sample container accommodating cavity 121 is slightly larger than the outer diameter of the sample container. An abutting portion 122 is provided at the bottom of the sample container accommodating chamber 121, and the abutting portion 122 is configured such that when the sample container 2 is accommodated in the sample container accommodating chamber 121, the sample container 2 Abutting on the abutting portion 122, so that the central axis A3 of the sample container 2 and the rotation axis A1 of the sample container holder 12 or the rotation axis A1 of the sample container 2 form an acute angle, especially The intersection of the central axis A3 of the sample container and the projection line of the rotation axis A1 of the sample container holder 12 in the vertical plane is located above the bottom of the cavity of the sample container 2 that contains the sample 3. In this embodiment, the abutment portion 122 is integrally formed on the bottom of the sample container accommodating chamber 121 of the sample container holder 12. Of course, in another embodiment not shown, the abutment portion 122 may be detachably fixed to the bottom of the sample container accommodating chamber 121 of the sample container holder 12, such a structure can facilitate replacement of the abutment To accommodate different sample containers.

In the embodiments shown in FIGS. 5 to 7, the sample container seat 12 is configured as a receiving cavity in the form of a hole, but the present invention is not limited thereto. As shown in FIG. 8, the sample container holder 12 can also be configured as a cavity 121 surrounded by a plurality of (three or more) cylinders, and the sample container 12 can be fixedly accommodated in the cavity 121. In the embodiment of FIG. 8, the bottom of the cavity 121 is also provided with an abutment portion 122 configured such that when the sample container 2 is accommodated in the cavity 121, the sample The container 2 abuts on the abutment portion 122 so that the central axis of the sample container 2 forms an acute angle with the rotation axis of the sample container holder 12 or the rotation axis of the sample container 2. For other features in the embodiment of FIG. 8, refer to the description of the embodiments shown in FIGS. 5 to 7, and details are not described herein again.

In the embodiments shown in FIGS. 5 to 7, the sample container seat 12 further includes a rotating shaft fixing hole 123 below, and the driving rotating shaft of the driving mechanism 13 is inserted into the rotating shaft fixing hole 13 and fixed to the rotating shaft fixing hole 13 Connected so that the sample container seat 12 rotates as the drive shaft of the drive mechanism 13 rotates. In this way, the axis A1 of the rotation shaft fixing hole 123 is the rotation axis of the sample container holder 12. As shown in FIG. 5, the axis A1 of the rotating shaft fixing hole 123 and the central axis A2 of the sample container accommodating cavity 121 may not coincide, that is, the sample container accommodating cavity 121 may be eccentrically disposed relative to the rotation axis of the sample container holder 12, The eccentricity d may be, for example, 0 mm to 5 mm, and the preferred range is 1 mm to 2 mm. In the case of an eccentric setting, the sensor 14 can also be used to locate the initial position of the sample container holder 12.

The role of the abutting portion 122 is to keep the sample container 2 inclined relative to the sample container accommodating cavity 121 when being put into the sample container accommodating cavity 121, that is, to make the sample container 2 be put into the sample container accommodating cavity 121 when the sample The central axis A3 of the container 2 and the rotation axis A1 of the sample container holder 12 form an acute angle α, as shown in FIG. 6. In this embodiment, when the sample container 2 is fixedly accommodated in the sample container holder 12, the central axis A3 of the sample container 2 and the rotation axis A1 of the sample container holder 12 are in the same plane, that is, the central axis of the sample container 2 A3 intersects the axis of rotation A1 of the sample container holder 12. In some preferred embodiments, the included angle α of the acute angle may be less than or equal to about 45°, and preferably may be about 2° to about 15°.

In addition, as shown in FIG. 6, the intersection point P of the central axis A3 of the sample container 2 and the rotation axis A1 of the sample container holder 12 is located above the bottom of the sample chamber of the sample container 2. Specifically, the intersection point P between the rotation axis A1 of the sample container holder 12 and the central axis A3 of the sample container 2 may be located above the entrance of the sample chamber of the sample container 2, as shown in FIG. 10; or as shown in FIG. 11, The intersection point P between the axis of rotation A1 of the sample container holder 12 and the central axis A3 of the sample container 2 is located between the inlet of the sample container 2 and the bottom of the sample container 2.

When the driving mechanism 13 drives the sample container holder 12 to rotate about its rotation axis A1, the sample (such as a blood sample) 3 in the sample container 2 changes from the state shown in FIG. 6 to the state shown in FIG. 7. Under the effect of centrifugal force, the sample 3 is thrown away from the rotation axis A1 of the sample container seat 12 and rises along the inner wall of the cavity of the sample container 2.

In this case, as shown in FIG. 8, the force analysis of the sample on the inner wall of the sample container 2 is as follows: According to the principles of acting force and reaction force, the sample unit S on the inner wall of the sample container 2 is subjected to The force F perpendicular to the inner wall of the cavity can be decomposed into component forces F1 and F2, where F1 = F × cos α, F2 = F × sin α, and further F2 = F1 × tan α. The force F1 provides a centripetal force for the sample unit S to rotate about the rotation axis A1, and the force F2 can prevent the sample unit S from rising along the inner wall of the cavity of the sample container 2.

According to the centripetal force formula F1=Δmω ² r, it can be seen that the centripetal force F1 is proportional to the square of the angular velocity ω of the sample unit S rotating around the rotation axis A1, where F1 is the centripetal force required for the sample unit S to rotate about the rotation axis A1, and Δm is the sample unit S The mass, ω is the angular velocity of the sample unit S around the axis of rotation A1, r is the radius of the sample unit S around the axis of rotation A1. Those skilled in the art should understand that due to the fluidity of the liquid, the greater the angular velocity ω of the liquid rotation, the more serious the tendency of the liquid to leave the center of rotation, that is, the tendency of the sample unit S to rise along the inner wall of the chamber of the sample container 2 as it rotates around The angular velocity ω of rotation of the axis A1 increases and increases. However, further derivation shows that F2 = Δmω ² r × tan α. As the angular velocity ω increases, the force F2 that prevents the sample unit S from rising along the inner wall of the container cavity of the sample container 2 also increases, and the volume of the sample unit S along the sample container 2 The upward trend of the inner wall of the cavity cancels out. Therefore, not only by adjusting the angular velocity ω, that is, the driving rotation speed of the driving mechanism, can the requirements of sample mixing be satisfied, but also by adjusting the acute angle α to control the liquid level height when the sample rotates. That is to say, by reasonably adjusting the two parameters of angular velocity ω and included angle α, a dynamic balance can be finally achieved, that is, the sample unit S can rotate around the rotation axis A1 at a larger angular velocity ω, and It does not cause the liquid level to climb too high, and thus can prevent the sample from overflowing the sample container 2. Moreover, by suppressing the height of the liquid surface when the sample rotates, the residual amount of the hanging liquid of the sample 3 on the inner wall of the chamber of the sample container 2 is reduced, thereby reducing the sample loss.

As described above, when the sample 3 in the sample container 2 is mixed uniformly, the center axis A3 of the sample container 2 relative to the axis of rotation of the sample container base 12 when the sample container 2 is placed in the sample container base 12 A1 is inclined, that is, by staggering the rotation axis A1 of the sample container holder 12 and the central axis A3 of the sample container 2, ie forming an acute angle, it is preferable that the rotation axis A1 of the sample container holder 12 and the central axis A3 of the sample container 2 The intersection point is located above the bottom of the sample chamber of the sample container 2, which can not only prevent the sample from spilling, but also reduce the wall loss when the sample 2 is mixed. This is especially true for samples with a small sample volume. For example, peripheral blood samples are extremely important, because excessive wall loss will affect the reliability of sample aspiration after mixing.

Of course, the mixing device 1 proposed in the embodiment of the present invention can also be used to mix a sample container containing a larger sample volume. Therefore, the sample container 2 that can be placed in the sample container accommodating cavity 121 of the sample container holder 12 is not limited to a small amount of blood collection tube containing a small amount of sample, such as a small amount of peripheral blood, or a vacuum containing more venous blood Blood collection tubes can also be other types of sample containers, such as sample containers used to contain urine, ascites, cerebral spinal cord, pleural effusion, etc. However, for a micro blood collection tube containing a small amount of samples, such as peripheral blood, the mixing device of the embodiment of the present invention has obvious advantages.

In addition, the sample container 2 that can be put into the sample container accommodating chamber 121 of the sample container holder 12 may be either a closed lid container or an open lid container. Since the mixing device 1 proposed in the embodiment of the present invention can effectively prevent the sample in the sample container 2 from flowing toward the opening of the cavity of the sample container 2 when the sample 3 in the sample container 2 is mixed, even if the lid is opened There is no need to worry about the risk of spilling when mixing the container.

It should be particularly noted that the embodiment of the present invention achieves better mixing by tilting the central axis of the sample container 2 relative to the axis of rotation of the sample container holder 12 when the sample container 2 is contained in the sample container holder 12 The effect, especially to prevent the sample from overflowing the sample container 2 and to suppress the liquid level height when the sample rotates, therefore, it is best to make the intersection point of the rotation axis of the sample container 2 (that is, the rotation axis of the sample container holder 12) and the central axis of the sample container 2 P is located above the bottom of the sample chamber of the sample container 2, as shown in FIGS. 10 and 11. When the intersection point P of the rotation axis of the sample container 2 and the central axis of the sample container 2 is located below the bottom of the sample container cavity of the sample container 2, as shown in FIG. 11, the direction of the component force F2 will be along the inner wall of the sample container 2 cavity Upwards, this may cause the sample to overflow the sample container 2 during sample mixing. If this embodiment is adopted, the risk of sample overflow can be avoided by reducing the rotation speed.

In some embodiments not shown, when the sample container 2 is fixedly accommodated in the sample container holder 12, the central axis of the sample container 2 and the rotation axis of the sample container holder 12 may also lie in different planes, in this case Next, the central axis of the sample container 2 and the projection line of the rotation axis of the sample container holder 12 in the vertical plane form an angle α of an acute angle, preferably the center axis of the sample container 2 and the rotation axis of the sample container holder 12 are in the vertical plane The intersection of the projection lines in is located above the bottom of the sample chamber of the sample container 2. That is to say, when the sample container 2 is fixedly accommodated in the sample container holder 12, the rotation axis A1 of the sample container holder 12 and the central axis A3 of the sample container 2 may be two straight lines that are neither parallel nor intersecting , The intersection point of the projection line of the axis A1 and the axis A3 in the vertical plane is located above the bottom of the sample chamber of the sample container 2, so that when the driving mechanism 13 drives the sample container holder 12 to rotate around the rotation axis A1, the sample container The sample 3 in 2 is subjected to the component force of the side wall of the sample container 2 toward the bottom of the cavity of the sample container 2, and then the angular velocity ω of the sample container base 12 rotating around the rotation axis A1 and the sample container 2 are set by reasonably setting To adjust the mixing effect and the height of the sample liquid level in the sample container 2 during mixing.

Next, a second embodiment of the sample container holder 12 of the present invention will be described with reference to FIGS. 13 and 14, wherein FIG. 13 is a cross-sectional view of the second embodiment of the sample container holder 12 of the sample mixing device 1 of the present invention, and FIG. 14 It is a cross-sectional view of the sample container holder 12 in FIG. 13 together with the sample container 2 accommodated in its sample container accommodating cavity when it is at rest, in which the sample container 2 contains a sample. This second embodiment is different from the first embodiment shown in FIGS. 5 to 7 in that the axis of the rotating shaft fixing hole 123 of the sample container holder 12 coincides with the central axis of the sample container accommodating chamber 121, that is The sample container accommodating chamber 121 is arranged concentrically with respect to the rotation axis of the sample container seat 12. In this second embodiment, the sample container 2 is also inclined by providing the contact portion 122 in the sample container accommodating chamber 121. As shown in FIG. 14, the rotation axis A1 of the sample container holder 12 and the sample The intersection point P of the central axis A3 of the container 2 is also located above the bottom of the sample chamber of the sample container 2. When the sample container holder 12 rotates, the sample can also be prevented from overflowing the sample container 2 and the liquid level during sample rotation can be suppressed.

In addition, a third embodiment of the sample container holder 12 of the present invention will be described with reference to FIGS. 15 and 16, wherein FIG. 15 is a cross-sectional view of the third embodiment of the sample container holder 12 of the sample mixing device 1 of the present invention, and FIG. 16 is a cross-sectional view of the sample container holder 12 in FIG. 15 together with the sample container 2 accommodated in its sample container accommodating cavity when at rest, in which the sample container 2 contains a sample. This third embodiment differs from the first embodiment shown in FIGS. 5 to 7 or the second embodiment shown in FIGS. 13 and 14 in that in this third embodiment, the sample container holder 12 is provided There is a sample container accommodating cavity 121 and a motor shaft fixing hole 123, but the sample container accommodating cavity 121 is not provided with an abutting portion 122 for abutting the sample container 2. Instead of the provision of the abutment portion 122, the sample container accommodating chamber 121 is constructed such that the central axis A2 and the rotation axis of the sample container holder 12 (in this embodiment, the axis line A1 of the shaft fixing hole 123) The acute angle α is formed so that when the sample container 2 is accommodated in the sample container accommodating chamber 121, the central axis A3 of the sample container 2 and the rotation axis A1 of the sample container seat form an acute angle The angle α is preferably such that the intersection point P of the projection line of the central axis A3 of the sample container 2 and the rotation axis A1 of the sample container seat in the vertical plane is located above the bottom of the sample chamber of the sample container 2. Wherein, the included angle α of the acute angle may be less than or equal to about 45°, preferably from about 2° to about 15°. As a result, it is possible to prevent the sample from overflowing the sample container 2 when the sample container holder 12 rotates and suppress the liquid level height when the sample rotates.

In some embodiments, as shown in FIG. 17, the inlet of the sample container fixing hole 121 of the sample container fixing base 12 of the mixing device 1 is set into a tooth shape, for example, a dentate shape, and the inner diameter of the tooth shape is larger than that of the sample container 2 Diameter, so that when the barcode label of the sample container 2 is not firmly adhered and the edge of the barcode label paper is opened, when the sample container is transported to the sample container holder 12, the sample container can not be placed in the sample container of the sample container holder 12 Probability of fixing hole 121.

On the other hand, as shown in FIG. 18, the present invention also describes a sample analysis system 100, especially an automatic blood analysis system with a peripheral blood measurement mode, such as a single automatic blood analyzer or one or more blood Automatic analyzer blood analysis pipeline system. The sample analysis system includes:

The sample mixing device 110 is configured to mix the sample in the sample container and includes a sample container seat for accommodating the sample container and a driving mechanism for driving the sample container seat to rotate about its rotation axis, wherein, The sample container holder is configured such that when the sample container is accommodated in the sample container holder, the central axis of the sample container forms an acute angle with the rotation axis of the sample container holder;

A control device (not shown) is configured to communicate with the drive mechanism to control the drive mechanism to drive the sample container holder to rotate about its axis of rotation.

The structure of the sample mixing device 110 may be completely the same as the structure of the mixing device shown in FIGS. 1 to 17 of the foregoing embodiment, and details are not described herein again.

The control device may be any device capable of issuing control commands, such as a microcontroller or the like. The communication connection includes a wireless communication connection, such as a WIFI connection, and a wired communication connection, such as a direct connection through a USB interface or a network port.

Further, the control device may be configured to control the driving mechanism in the following manner:

Further, the sample mixing device further includes a sensor for detecting the rotation state of the sample container holder, the sensor is in communication connection with the control device, so as to transmit the detected rotation state of the sample container holder to The control device, which can adjust the driving parameters of the driving mechanism according to the rotation state of the sample container seat or can determine the sample container seat or the drive according to the rotation state of the sample container seat Whether the organization is malfunctioning. For example, the sensor may be used to determine whether the sample container base is rotated, so that the control device can determine whether the drive mechanism or the sample container base is damaged based on this. Or the sensor can also be used to determine whether the rotation speed of the base of the sample container is as expected. If the predetermined rotation speed is not reached, it will affect the mixing effect.

Further, the sample analysis system further includes:

A first conveying device configured to be communicatively connected with the control device and used to place the sample container into the sample container seat under the control of the control device;

In the embodiment of the present invention, the first conveying device is to place the sample container to be mixed in the sample container seat, so as to mix the sample in the sample container, and the second conveying device is to mix the sample container after mixing Remove from the sample container holder so that the next sample to be mixed is contained in the sample container holder.

Further, the first conveying device and the second conveying device may be configured as the same device or different devices.

In the embodiment shown in FIG. 18, the first transport device and the second transport device are configured as the same sample container transport device 120.

In addition, the sample analysis system 100 may further include a sample suction device 130 configured to communicate with the control device and draw the sample mixed through the sample mixing device 110 under the control of the control device.

Further, the sample analysis system 100 may further include a sample rack transport device 140 configured to transport a sample rack 150 that can load at least one sample container 2.

The working process of the sample analysis system 100 according to the present invention is as follows: the sample rack transport device 140 transports the sample rack 150 loaded with the sample container 2 to the mixing position of the sample analysis system 100, and then the sample container handling device 120 transfers the sample container 2 from The sample rack 150 is transported to the sample mixing device 110, the sample mixing device 110 mixes the sample 3 in the sample container 2, and then the sample container transport device 120 transfers the sample container 2 from the sample mixing device 110 back The sample rack 150, and finally the sample rack conveying device 140 further sends the sample rack 150 where the sample container 2 loaded with the mixed sample is located to the sample suction position of the sample analysis system 100, and the sample suction device 130 performs sample suction to perform sample Detection.

In yet another aspect, the present invention also describes a sample mixing method, especially the sample mixing method implemented by the above-mentioned sample mixing device 1.

FIG. 19 shows a schematic flowchart of a first embodiment of a sample mixing method 200 of the present invention. The sample mixing method 200 includes:

201. Place the sample container into the rotatable sample container seat (using a manipulator or manual) so that the central axis of the sample container and the rotation axis of the sample container seat form an acute angle, preferably such that The intersection of the central axis of the sample container and the projection line of the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container cavity of the sample container, and it is more preferable that the central axis of the sample container and the The acute angle formed by the axis of rotation of the sample container holder is less than or equal to about 45°, preferably in the range of about 2° to about 15°.

202. The driving mechanism drives the sample container base to rotate around its rotation axis to perform sample mixing operation.

In addition, after the sample mixing operation is completed, the driving mechanism is stopped, especially after the sample is returned to the bottom of the cavity of the sample container, the sample container is taken out, and then the sample container The sample is sucked, or the sample in the sample container is directly sucked by the sampling device.

The embodiment of the present invention drives the sample container holder to drive the sample container containing the sample to rotate around the rotation axis by the driving mechanism, so that the sample in the sample container is uniformly mixed, wherein the central axis of the sample container is inclined with respect to the rotation axis of the sample container holder. When the driving mechanism drives the sample container holder to rotate, the sample in the sample container rotates along the inner wall of the sample container cavity and climbs toward the sample container cavity opening of the sample container, and when the sample container holder stops rotating, it faces the inner wall of the sample container cavity The sample cavity opening of the sample container climbs a certain distance and the sample flows back to the bottom of the sample container cavity. Therefore, in the embodiment of the present invention, the sample 3 in the sample container is rotated and climbed and refluxed to achieve uniform mixing of the sample.

FIG. 20 shows a schematic flowchart of the first mixing method in step 202 of the sample mixing method 200, where step 202 includes:

2021a. The sample container holder is driven by the driving mechanism to rotate around the axis of rotation in the first direction;

2021b. The sample container holder is driven by the driving mechanism to rotate in a second direction opposite to the first direction about its axis of rotation.

2021c. Stop the driving mechanism to stop the rotation of the sample container seat and wait for the sample in the sample container to return to the bottom of the container cavity of the sample container.

21 shows a schematic flowchart of a second mixing method in step 202 of the sample mixing method 200, where step 202 includes:

2022a, the sample container holder is driven to rotate around its axis of rotation by the driving mechanism;

2022b. Stop the driving mechanism to stop the rotation of the sample container seat and wait for the sample in the sample container to return to the bottom of the cavity of the sample container;

2022c.

Repeat

2022a and 2022b multiple times.

22 shows a schematic flowchart of a third mixing method in step 202 of the sample mixing method 200, where step 202 includes:

2023a. The driving mechanism drives the sample container base to rotate in a first direction around its axis of rotation;

2023b. Stop the driving mechanism to stop the rotation of the sample container seat and wait for the sample in the sample container to return to the bottom of the container cavity of the sample container;

2023c. The sample container holder is driven by the driving mechanism to rotate around its axis of rotation in a second direction opposite to the first direction;

2023d. Stop the driving mechanism to stop the rotation of the sample container seat and wait for the sample in the sample container to return to the bottom of the cavity of the sample container.

Preferably, in this embodiment, 2023a to 2023d can also be repeated multiple times.

By rotating the sample container in the sample container holder 12 in the forward direction and in the direction, a better mixing effect can be obtained for the micro sample, especially the micro blood sample.

According to the present invention, in particular, by rotating the sample in the sample container and repeatedly climbing and refluxing, a better sample mixing effect can be obtained.

FIG. 23 shows a schematic flowchart of a second embodiment of a sample mixing method 300 of the present invention.

Steps

301 and 302 of the sample mixing method 300 and

steps

201 and 302 of the sample mixing method 200 shown in FIG. 19 Same as 202, the sample mixing method 300 further includes:

303. The sensor detects the rotation state of the sample container holder;

304. Adjust the driving parameters of the driving mechanism, such as the rotation speed, according to the rotation state; and/or determine whether the sample container holder or the driving mechanism fails according to the rotation state.

An embodiment of the present invention also describes a control device for a sample analysis system having the above-mentioned sample mixing device, and the control device is in communication connection with a driving mechanism and a sensor (if any) of the sample mixing device .

24 is a schematic structural diagram of a control device 400 according to an embodiment of the present invention. The control device 400 includes at least one processor 401 and a memory 402. The memory 402 stores instructions executable by the at least one processor 401. When executed by the at least one processor 401, the sample analysis system executes the steps of the above sample mixing method.

In addition, the control device 400 may further include at least one network interface 404 and user interface 403. The various components in the control device 400 are coupled together via a bus system 405. Understandably, the bus system 405 is used to implement connection and communication between these components. In addition to the data bus, the bus system 405 also includes a power bus, a control bus, and a status signal bus. However, for the sake of clarity, various buses are marked as the bus system 405 in FIG. 24.

The user interface 403 may include a display, a keyboard, a mouse, a trackball, a click wheel, buttons, buttons, a touch panel, or a touch screen.

It can be understood that the memory 402 may be a volatile memory or a non-volatile memory, and may also include both volatile and non-volatile memory. Among them, the non-volatile memory can be read-only memory (ROM, Read Only Memory), programmable read-only memory (PROM, Programmable Read-Only Memory), erasable programmable read-only memory (EPROM, Erasable Programmable Read- Only Memory), Electrically Erasable Programmable Read Only Memory (EEPROM, Electrically Erasable Programmable Read-Only Memory), Magnetic Random Access Memory (FRAM, Ferromagnetic Random Access Memory), Flash Memory (Flash) Memory, Magnetic Surface Memory , Compact disc, or read-only compact disc (CD-ROM, Compact, Read-Only Memory); the magnetic surface memory can be a disk storage or a tape storage. The volatile memory may be a random access memory (RAM, Random Access Memory), which is used as an external cache. By way of example but not limitation, many forms of RAM are available, such as static random access memory (SRAM, Static Random Access Memory), synchronous static random access memory (SSRAM, Synchronous Static Random Access Memory), dynamic random access Memory (DRAM, Dynamic Random Access), Synchronous Dynamic Random Access Memory (SDRAM, Synchronous Dynamic Random Access Memory), Double Data Rate Synchronous Dynamic Random Access Memory (DDRSDRAM, Double Data Rate, Synchronous Dynamic Random Access Random Access Memory), enhanced Type of synchronous dynamic random access memory (ESDRAM, Enhanced Synchronous Dynamic Random Access Memory), synchronous connection dynamic random access memory (SLDRAM, SyncLink Dynamic Random Access Memory), direct memory bus random access memory (DRRAM, Direct Rambus Random Access Random Access Memory ). The memory 402 described in the embodiments of the present invention is intended to include these and any other suitable types of memory.

The memory 402 in the embodiment of the present invention includes, but is not limited to: tri-state content addressable memory, static random access memory capable of storing various types of data such as received sensor signals to support the operation of the control device 400.

The processor 401 in the embodiment of the present invention may be a central processing unit (Central Processing Unit, CPU), or other general-purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC ), off-the-shelf programmable gate array (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor can be a microprocessor or the processor can also be Any conventional processor, etc.

Embodiments of the present invention also provide a computer-readable storage medium, for example, a memory 402 including a computer program, which can be executed by the processor 401 of the controller 400, so that the sample analysis system executes the steps of the sample mixing method . The computer-readable storage medium may be a memory such as FRAM, ROM, PROM, EPROM, EEPROM, Flash Memory, magnetic surface memory, optical disk, or CD-ROM; it may also be various devices including one or any combination of the foregoing memories.

The features mentioned above can be combined with each other as long as they are meaningful within the scope of the present invention. The advantages and features described for the sample mixing device are applied in a corresponding manner to the corresponding system and the corresponding method.

The above are only the specific embodiments of the present invention, but the scope of protection of the present invention is not limited to this. Any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the present invention. It should be covered by the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

A sample mixing device includes a sample container seat for accommodating a sample container and a driving mechanism for driving the sample container seat to rotate about its axis of rotation, wherein the sample container seat is configured such that when the sample container When fixedly accommodated in the sample container holder, the central axis of the sample container and the rotation axis of the sample container holder form an acute angle.
The sample mixing device according to claim 1, wherein the sample container holder is configured such that when the sample container is fixedly received in the sample container holder, the central axis of the sample container and the The intersection point of the projection line of the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container containing the sample chamber.
The sample mixing device according to claim 2, wherein the sample container holder is configured such that when the sample container is fixedly received in the sample container holder, the central axis of the sample container is The rotation axis of the sample container seat is located in the same plane, the central axis of the sample container intersects the rotation axis of the sample container seat, and the intersection point is located above the bottom of the cavity of the sample container.
The sample mixing device according to claim 2, wherein the sample container holder is configured such that when the sample container is fixedly received in the sample container holder, the central axis of the sample container is The rotation axes of the sample container seat are located in different planes, and the intersection of the central axis of the sample container and the projection line of the rotation axis of the sample container seat in the vertical plane is above the bottom of the cavity of the sample container.
The sample mixing device according to any one of claims 1 to 4, wherein the sample container holder has a sample container accommodating cavity capable of fixedly accommodating the sample container, the sample container accommodating cavity is configured When the sample container is fixedly accommodated in the sample container accommodating cavity, the central axis of the sample container and the rotation axis of the sample container seat form an acute angle, preferably the middle of the sample container The intersection of the axis and the projection line of the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container containing cavity of the sample container.
The sample mixing device according to claim 5, wherein an abutment portion is provided in the sample container accommodating cavity, the abutment portion is configured such that when the sample container is accommodated in the sample container accommodation When in the cavity, the sample container abuts on the abutting portion, so that the central axis of the sample container and the rotation axis of the sample container seat form an acute angle, preferably the central axis of the sample container is The intersection point of the projection line of the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container containing cavity of the sample container.
The sample mixing device according to claim 6, wherein the abutting portion is integrally formed at the bottom of the sample container accommodating cavity of the sample container holder, or the abutting portion is detachably fixed The bottom of the sample container accommodating cavity of the sample container holder.
The sample mixing device according to claim 5, wherein the sample container accommodating cavity is configured such that the central axis forms an acute angle with the rotation axis of the sample container seat, so that when the sample container is fixedly When being accommodated in the sample container accommodating cavity, the central axis of the sample container forms an acute angle with the rotation axis of the sample container seat, preferably the rotation of the sample container central axis and the sample container seat The intersection point of the projection line of the axis in the vertical plane is located above the bottom of the sample container containing the sample chamber.
The sample mixing device according to any one of claims 5 to 8, wherein the sample container accommodating cavity is configured as an accommodating cavity in the form of a hole, or the sample container accommodating cavity is configured by a plurality of columns A cavity surrounded by a body, the sample container can be fixedly accommodated in the cavity.
The sample mixing device according to any one of claims 1 to 10, wherein the included angle between the central axis of the sample container and the axis of rotation of the sample container seat is less than or equal to about 45°, preferably In the range of about 2° to about 15°.
The sample mixing device according to any one of claims 1 to 10, wherein the driving mechanism has a driving shaft, and the sample container seat is directly fixed on the driving shaft or indirectly through a transmission mechanism The drive shaft is connected in rotation; or

The driving mechanism drives the runner with an elastic outer peripheral washer, and the outer peripheral washer contacts the outer periphery of the sample container seat to provide frictional force to rotate the sample container seat.
The sample mixing device according to claim 11, wherein the sample container seat includes a rotating shaft fixing hole connected to a driving rotating shaft of the driving mechanism, the driving rotating shaft of the driving mechanism is inserted into the rotating shaft fixing hole and It is fixedly connected with the rotating shaft fixing hole, so that the sample container seat rotates as the driving rotating shaft of the driving mechanism rotates.
The sample mixing device according to claim 12, wherein the rotating shaft fixing hole and the sample container accommodating cavity are coaxially arranged; or the rotating shaft fixing hole and the sample container accommodating cavity are eccentrically arranged, the amount of eccentricity It is 0 mm to about 5 mm, preferably about 1 mm to about 2 mm.
The sample mixing device according to any one of claims 1 to 13, wherein the driving mechanism is configured as a motor, such as a stepper motor, or a DC motor, or a servo motor.
The sample mixing device according to any one of claims 1 to 14, wherein the sample mixing device further comprises a sensor for detecting the rotation state of the sample container holder, for example, a photoelectric sensor, a reflective type The photoelectric sensor, Hall sensor, and capacitance sensor are preferably through-beam photoelectric sensors.
The sample mixing device according to claim 15, wherein the sample mixing device is provided with a sensing part and a notch in the sensing area of the sensor, and the sample mixing seat is driven to rotate by the driving mechanism When the sensing portion and the notch enter the sensing area of the sensor alternately, an output pulse signal of the sensor is generated for detecting the rotation state of the sample container holder.
A sample mixing system includes a sample container for containing a sample and a sample mixing device for mixing the sample in the sample container, wherein the sample mixing device is according to any one of claims 1 to 16. One structure.
The sample mixing system according to claim 17, wherein the sample container is configured as a micro blood collection tube for containing peripheral blood; and/or the sample container is configured as a closed container or an open container.
A sample analysis system, including:

A sample mixing device, especially the sample mixing device according to any one of claims 1 to 16, is configured to mix a sample in a sample container and includes a sample container holder for accommodating the sample container and A drive mechanism for driving the sample container holder to rotate about its axis of rotation, wherein the sample container holder is configured such that when the sample container is accommodated in the sample container holder, the central axis of the sample container Forming an acute angle with the axis of rotation of the sample container holder;

The control device is configured to be communicatively connected with the driving mechanism to control the driving mechanism to drive the sample container holder to rotate around its rotation axis.
The sample analysis system according to claim 19, wherein the control device is configured to control the driving mechanism in the following manner:

Causing the drive mechanism to drive the sample container holder to rotate in the same direction about its axis of rotation, preferably the drive mechanism to drive the sample container holder to periodically rotate in the same direction about its axis of rotation; or

The driving mechanism drives the sample container seat to rotate alternately in a first direction and a second direction opposite to the first direction about its axis of rotation, preferably, the driving mechanism drives the sample container seat around it After the rotation axis rotates in the first direction for a predetermined period of time, the drive mechanism is stopped, and after the sample returns to the bottom of the cavity of the sample container, the drive mechanism is turned on to drive the sample container seat around its rotation axis Turn in a second direction opposite to the first direction.
The sample analysis system according to claim 19 or 20, wherein the sample mixing device further comprises a sensor for detecting the rotation state of the sample container holder, the sensor can be communicatively connected with the control device in order to The detected rotation state of the sample container holder is transmitted to the control device, and the control device can adjust the driving parameters of the driving mechanism according to the rotation state of the sample container holder or can be adjusted according to the sample container holder Rotate the state to determine whether the sample container holder or the drive mechanism is malfunctioning.
The sample analysis system according to any one of claims 19 to 21, wherein the sample analysis system further comprises:

A first conveying device configured to be communicatively connected with the control device and used to place the sample container into the sample container seat under the control of the control device;

The second transport device is configured to be communicatively connected to the control device and used to remove the sample container from the sample container seat under the control of the control device.
The sample analysis system according to claim 22, wherein the first transport device and the second transport device are configured as the same device or different devices.
A sample mixing method, the method includes:

The sample container is fixedly placed in the rotatable sample container seat, so that the central axis of the sample container and the rotation axis of the sample container seat form an acute angle, preferably the central axis of the sample container and the The intersection point of the projection line of the rotation axis of the sample container seat in the vertical plane is located above the bottom of the sample container containing the sample container;

The driving mechanism drives the sample container base to rotate around its axis of rotation to perform sample mixing operation.
The sample mixing method according to claim 24, wherein the sample container is fixedly placed in the rotatable sample container holder so that an acute angle formed by the central axis of the sample container and the rotation axis of the sample container holder The included angle is less than or equal to about 45°, preferably in the range of about 2° to about 15°.
The sample mixing method according to claim 24 or 25, wherein the step of driving the sample container holder to rotate about its axis of rotation by the driving mechanism to perform the sample mixing operation includes:

The sample container holder is driven to rotate in the same direction around its axis of rotation by the drive mechanism, preferably the sample container holder is driven by the drive mechanism to periodically rotate in the same direction about its axis of rotation; or

The sample container holder is driven by the driving mechanism to rotate alternately in a first direction and a second direction opposite to the first direction around its axis of rotation. Preferably, the sample container holder is driven by the driving mechanism about its axis of rotation along the first After rotating in one direction for a predetermined period of time, stop the driving mechanism and wait for the sample to flow back to the bottom of the cavity of the sample container before turning on the driving mechanism to drive the sample container holder around its axis of rotation in the reverse direction The second direction rotates in the first direction.
The sample mixing method according to any one of claims 24 to 26, wherein the method further comprises:

The sensor detects the rotation state of the sample container holder;

Adjust the drive parameters of the drive mechanism, such as the speed, according to the rotation state; and/or

According to the rotation state, it is determined whether the sample container holder or the driving mechanism is malfunctioning.
A control device for a sample analysis system, including:

At least one processor; and

A memory storing instructions executable by the at least one processor, which when executed by the at least one processor causes the sample analysis system to perform each of the methods according to any one of claims 24 to 27 step.
A computer-readable storage medium storing computer-executable instructions, which when executed by at least one processor of a sample analysis system, causes the sample analysis system to perform any one of claims 24 to 27 The steps of the method.