WO2020125554A1 - Spectrophotometer inspection system and method - Google Patents

Spectrophotometer inspection system and method Download PDF

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Publication number
WO2020125554A1
WO2020125554A1 PCT/CN2019/125192 CN2019125192W WO2020125554A1 WO 2020125554 A1 WO2020125554 A1 WO 2020125554A1 CN 2019125192 W CN2019125192 W CN 2019125192W WO 2020125554 A1 WO2020125554 A1 WO 2020125554A1
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Prior art keywords
light beam
detection system
light
spectrum
spectrophotometer
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PCT/CN2019/125192
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French (fr)
Chinese (zh)
Inventor
陈巍
祝铭
李剑平
张亮
周志盛
梁元博
赵强星
周轩
吕建成
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中国科学院深圳先进技术研究院
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Publication of WO2020125554A1 publication Critical patent/WO2020125554A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0162—Arrangements or apparatus for facilitating the optical investigation using microprocessors for control of a sequence of operations, e.g. test, powering, switching, processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices
    • G01N2201/0407—Batch operation; multisample devices with multiple optical units, e.g. one per sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices
    • G01N2201/0453—Multicell sequential and multitest, e.g. multiwavelength
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0636—Reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0638—Refractive parts
    • G01N2201/0639—Sphere lens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/105—Purely optical scan

Definitions

  • the invention belongs to the technical field of optical measurement, and particularly relates to a spectrophotometer detection system and a detection method thereof.
  • the spectrophotometer uses the absorption spectrum of the substance to infer the type and content of the substance component.
  • the basic law of spectrophotometry is Lambert-Beer law (Lambert-Beer law), which describes the relationship between the intensity of a substance's absorption of light at a certain wavelength and the concentration of the light-absorbing substance and the thickness of the liquid layer.
  • Lambert-Beer law Lambert-Beer law
  • Spectrophotometers often use xenon lamps, deuterium lamps, tungsten halogen lamps, etc. as light sources. These light sources have a relatively wide spectrum that can cover multiple wavelength bands. This type of incoherent light source has a certain intensity fluctuation during operation, which affects the stability of the absorption spectrometer of traditional structure. Furthermore, the spectral output characteristics of these light sources are not stable enough, that is, there will be a proportional difference in the output at different wavelengths. The instability of the energy output of the light source and the spectral output will introduce errors into the time-sharing spectrum. The method of adding a reference optical path is commonly used in the detection of scientific instruments to eliminate the influence of the instability of the energy output of the light source and the spectral output on the measurement results.
  • the reference optical path is generally a solvent that does not contain any solute components compared to the detection optical path containing the sample. Measure the test light path containing the sample first and then move the sample cell to measure the reference light path. Lambert-Beer law is applied to the reference spectrum measurement, the expression is: Abs ⁇ represents the absorbance at the wavelength ⁇ , I 0 ⁇ represents the spectrum collected by the reference optical path; I ⁇ represents the spectrum collected by the signal optical path. By this way of spectral subtraction, the influence of light source instability on the experimental results is eliminated.
  • the reference optical path There are two common ways of implementing the reference optical path. The first is to switch the reference optical path and the sample optical path by switching the position of the sample cell. In this way, there is a position deviation when resetting due to the movement accuracy of the machine, which reduces the measurement accuracy of the system. The second is to rely on multiple light sources or spectral detection modules to achieve the simultaneous acquisition of the reference optical path and the sample optical path. This approach will introduce new errors and reduce measurement accuracy, such as differences in the characteristics of different light sources and differences in the spectral response of different spectral detection modules. In addition, the dual light source or dual spectrum detection module also greatly increases the cost of the spectrophotometer detection system.
  • the measurement time is an important parameter for the spectrophotometer.
  • the single measurement time determines whether the spectrophotometer can be used for in-situ measurement, such as in-situ measurement of water quality parameters (chemical oxygen demand, total organic carbon, etc.). It can be satisfied that the measurement time is completed on the order of milliseconds, and the change of the water composition caused by the water flow can be almost ignored.
  • the measurement time also determines the reference optical path and the sample optical path in the successive measurement. The influence of the instability of the light source on the two measurement results ultimately affects the detection accuracy.
  • rapid measurement is one of the core features of spectrophotometers.
  • the patent application publication number is CN107941717A
  • the Chinese patent of the invention titled a static multi-sample cell spectrophotometer uses a linear structure lined up, and then relies on a stepper motor to move multiple sample cells Measure at the optical path.
  • the measurement of the "5-connected cell" structure must be in the order of minutes, and it takes up a lot of space, making it difficult to miniaturize the system.
  • the technical problem to be solved by the present invention is: how to realize rapid spectrophotometric measurement of multiple samples to be tested on a single light source and a single spectrum detection module.
  • a spectrophotometer detection system including:
  • Light source device for providing incident light beam
  • the sample containing device includes a plurality of containing chambers for containing the sample to be tested;
  • a beam scanning device for reflecting the incident beam through the accommodating cavity
  • the spectrum detection device is used for receiving the light beam passing through the accommodating cavity and acquiring the spectrum of the light beam passing through the accommodating cavity.
  • the spectrophotometer detection system further includes: a driving device for driving the light beam scanning device to perform time-division spatial scanning to reflect the incident light beam through each containing cavity in a time-division and space-division manner.
  • the spectrophotometer detection system further includes: a plurality of reflecting mirrors, the reflecting mirrors correspond to the accommodating cavity in one-to-one correspondence, the reflecting mirrors are used to reflect the incident beam onto the beam scanning device The light beam reflects through the corresponding cavity.
  • the light source device includes:
  • Light emitting element used to emit incident light beam
  • the beam shaper is used to quasi-parallel shape the incident light beam emitted by the light-emitting element.
  • the spectrophotometer detection system further includes: a first lens with positive refractive power, a focal point of the first lens coincides with the center of the beam scanning device, and the first lens is used to scan the beam scanning device The light beam reflected onto the incident light beam by time-sharing reflects through each accommodating cavity.
  • the light source device includes:
  • Light emitting element used to emit incident light beam
  • An optical element group with positive refractive power is used to focus the incident light beam emitted by the light-emitting element onto the center of the light beam scanning device.
  • the sample receiving device includes a receiving tray, a first window cover, and a second window cover, and the receiving tray is provided with a plurality of through holes penetrating the first and second opposing surfaces of the receiving tray ,
  • the first window cover is disposed on the first surface
  • the second window cover is disposed on the second surface, thereby forming a plurality of the receiving chambers.
  • the spectrum detection device includes:
  • each multimode optical fiber includes opposing first end surfaces and second end surfaces, the second end surfaces of the multiple multimode optical fibers are linearly arranged, and the first end surface and the receiving cavity are one by one Correspondingly, the first end surface is used to receive the light beam passing through the corresponding accommodating cavity;
  • the entrance slit meets a plurality of linearly arranged second end faces, and the optical fiber spectrometer is used to receive the light beam emitted from the second end face.
  • the invention also discloses a liquid in-situ spectrophotometer detection system, including a casing and the above spectrophotometer detection system, the spectrophotometer detection system is provided in the casing, and the casing is partially recessed to form a liquid in-situ measurement window , Opposite sides of the liquid in-situ measurement window are respectively provided with a first light-transmitting portion and a second light-transmitting portion, and the beam scanning device is further used to reflect incident light beams through the first light-transmitting portion and In the second light-transmitting part, the spectrum detection device is further used to receive the light beam passing through the second light-transmitting part and acquire the spectrum of the light beam in the second light-transmitting part.
  • the invention also discloses a detection method of the spectrophotometer detection system.
  • the detection method includes:
  • the light source device provides the incident beam
  • the beam scanning device reflects the incident light beam through the accommodating cavity of the sample accommodating device, and the accommodating cavity of the sample accommodating device contains the sample to be tested and the reference sample;
  • the spectrum detection device receives the light beam passing through the accommodating cavity, and acquires the spectrum of the light beam passing through the accommodating cavity, where the spectrum includes the spectrum of the sample to be tested and the spectrum of the reference sample;
  • the absorption spectrum of the sample to be measured is obtained according to the spectrum of the sample to be measured and the spectrum of the reference sample.
  • the spectrophotometer detection system disclosed by the invention realizes the rapid spectrophotometric measurement of multiple samples under the condition of a single light source and a single spectral module, eliminates the influence of light source intensity and output spectrum fluctuations on the spectrophotometer, improves the detection accuracy of the system, and can also make The structure is more compact and easy to integrate, while reducing costs.
  • FIG. 1 is a flowchart of a detection method of a spectrophotometer detection system according to an embodiment of the present invention
  • FIG. 2 is a schematic diagram of a spectrophotometer detection system according to Embodiment 1 of the present invention.
  • FIG. 3 is an exploded view of the structure of the sample holding device according to the first embodiment of the present invention.
  • FIG. 4 is a schematic diagram of the arrangement of the first end faces of multiple multimode optical fibers according to Embodiment 1 of the present invention.
  • FIG. 5 is a schematic diagram of the arrangement of second end faces of multiple multimode optical fibers according to Embodiment 1 of the present invention.
  • FIG. 6 is a schematic diagram of a spectrophotometer detection system according to Embodiment 2 of the present invention.
  • FIG. 7 is a schematic diagram of a liquid in-situ spectrophotometer detection system according to Embodiment 3 of the present invention.
  • the detection method of the spectrophotometer detection system includes the following steps S10 to S30:
  • Step S10 the light source device 10 provides an incident light beam
  • Step S20 The beam scanning device 30 reflects the incident beam through the accommodating cavity of the sample accommodating device 20, wherein the accommodating cavity of the sample accommodating device (20) contains the sample to be tested and the reference sample;
  • Step S30 The spectrum detection device 40 receives the light beam passing through the accommodating cavity and acquires the spectrum of the light beam passing through the accommodating cavity, where the spectrum includes the spectrum of the sample to be tested and the spectrum of the reference sample.
  • Step S40 Obtain the absorption spectrum of the sample to be tested according to the spectrum of the sample to be tested and the spectrum of the reference sample.
  • the spectrophotometer detection system includes a light source device 10, a sample holding device 20, a beam scanning device 30 and a spectrum detection device 40.
  • the light source device 10 is used to provide the incident light beam required for detection
  • the sample receiving device 20 includes a plurality of receiving cavities for receiving the sample to be tested
  • the beam scanning device 30 is used to reflect the incident light beam to pass through the receiving cavity
  • the spectrum detecting device 40 is used to receive the light beam passing through the accommodating cavity and acquire the spectrum of the light beam passing through the accommodating cavity.
  • the beam scanning device 30 is further used to reflect the incident light beam through each receiving cavity in a time-sharing manner, so as to realize the measurement of each sample to be measured.
  • the spectrophotometer detection system further includes a driving device 50 for driving the light beam scanning device 30 to rotate in a time-sharing manner, so that the light beam scanning device 30 reflects the incident light beam in a time-sharing manner.
  • the sample receiving device 20 includes a receiving tray 21, a first window cover 22 and a second window cover 23, and a plurality of opposing In the through holes 21a of the first surface and the second surface, the first window cover 22 is disposed on the first surface, and the second window cover 22 is disposed on the second surface, thereby forming a plurality of accommodating cavities. Further, the plurality of through holes 21a are arranged in an array to form a honeycomb structure. The cross-sectional shape of the through hole 21a is a regular hexagon, and the shape of the accommodating disk 21 is a cylindrical shape.
  • the thickness of the accommodating tray 21 is preferably 10 mm, the diameter of the circumscribed circle of the through hole 21 a is 5 mm, the material of the accommodating tray 21 is made of ordinary glass, and the material of the first window cover 22 and the second window cover 23 is preferably made of ultraviolet fused silica material . Further, both the first window cover 22 and the second window cover 23 adopt a detachable design.
  • the first window cover 22 is opened, different liquid samples are filled into the through holes 21a, and then the first window The cover 22 is closed, and finally the sample holding device 20 is put back into the system for measurement, and the first window cover 22 and the second window cover 23 are removed for cleaning at the same time when cleaning.
  • the light source device 10 includes a light emitting element 11 and a beam shaper 12, the light emitting element 11 is used to emit an incident light beam, and the beam shaper 12 is used to quasi-parallel shape the incident light beam emitted by the light emitting element 11.
  • the light-emitting element 11 preferentially uses a flashing xenon lamp.
  • the spectrophotometer detection system further includes a plurality of reflecting mirrors 60, the reflecting mirrors 60 correspond to the accommodating cavities one by one, and the reflecting mirrors 60 are used to reflect the incident beams reflected by the beam scanning device 30 to the beams passing thereon through the corresponding The cavity is accommodated. Wherein, both the surface of the reflecting mirror 60 and the reflecting surface of the beam scanning device 30 are coated with an ultraviolet enhanced reflecting film. Through the function of the reflecting mirror 60 and the beam scanning device 30, the incident beam can pass through the accommodating cavity quasi-parallel.
  • the spectrum detection device 40 includes a plurality of multimode optical fibers 41 and a fiber spectrometer 42, as shown in FIGS. 4 and 5, each multimode optical fiber 41 includes opposing first end surfaces 41a and second end surfaces 41b.
  • the second end surfaces 41b of the multi-mode optical fibers 41 are arranged linearly.
  • the first end surfaces 41a and the accommodating cavities are in one-to-one correspondence.
  • the first end surface 41a is used to receive the light beam passing through the corresponding accommodating cavities.
  • the entrance slit of the optical fiber spectrometer 42 is aligned with the plurality of linearly arranged second end faces 41b.
  • the optical fiber spectrometer 42 is used to receive the light beam emitted from the second end face 41b.
  • the spectrophotometer detection system further includes a second lens 80 for focusing the light beam passing through the corresponding accommodating cavity onto the first end surface 41a, and the first end surface 41a is located on the second lens 80 At the focal point, the first end surface 41a is perpendicular to the optical axis of the light beam passing through the corresponding receiving cavity.
  • the diameter of the second lens 80 is larger than the outer diameter of the accommodating disk 21.
  • the detection system of the spectrophotometer of the present invention controls the timing of the light-emitting element 11, the optical fiber spectrometer 42 and the optical scanning device 20 through a timing controller to realize time-sharing measurement.
  • the detection process of the spectrophotometer detection system is described below.
  • the optical fiber spectrometer 42 sends out a first trigger signal, which first triggers the optical scanning device 20 to reach the first preset angle. Then, the light-emitting element 11 is triggered to generate pulsed light, and the light beam emitted by the light-emitting element 11 becomes a quasi-parallel light after passing through the beam shaper 12.
  • the quasi-parallel beam passes through the optical scanning device 20 and is reflected to the mirror 60.
  • the mirror 60 directs the light beam to the containing cavity, and the light beam passes through the first sample to be measured in the containing cavity.
  • the light beam passes through the first sample to be tested and then reaches the second lens 80.
  • the second lens 80 is used to focus the light beam passing through the corresponding accommodating cavity onto the first end surface 41a.
  • the light beam passes through the multimode optical fiber 41 and exits from the second end surface 41b.
  • the entrance slit of the optical fiber spectrometer 42 coincides with the second end surface 41b, and the direction of the slit is the same as the direction in which the plurality of second end surfaces 41b are linearly arranged.
  • the measurement results are obtained through data analysis to obtain the first spectrum.
  • the optical fiber spectrometer 42 sends out a second trigger signal, which first triggers the optical scanning device 20 to reach the second preset angle.
  • the subsequent steps are the same as in the first measurement, and the second spectrum is finally obtained.
  • the light source device 10 includes a light-emitting element 11 and an optical element group 13 with positive refractive power.
  • the optical element group 13 may be a lens group or a concave mirror group, and the light-emitting element 11 is used to emit For the incident light beam, the optical element group 13 is used to focus the incident light beam emitted by the light-emitting element 11 onto the center of the beam scanning device 30.
  • the spectrophotometer detection system further includes a first lens 70 having positive refractive power, the focal point of the first lens 70 coincides with the center of the beam scanning device 30, and the first lens 70 is used to reflect the beam scanning device 30 in a time-sharing manner.
  • the light beam onto which the light beam is reflected reflects through each accommodating cavity. This allows the incident beam to pass through the receiving cavity quasi-parallel.
  • the other parts of the second embodiment are the same as those of the first embodiment, and will not be repeated here.
  • the detection process of the spectrophotometer detection system may also be:
  • the optical fiber spectrometer 42 sends out a first trigger signal, which first triggers the optical scanning device 20 to reach the first preset angle. Then, the light-emitting element 11 is triggered to generate pulsed light. The light beam emitted by the light-emitting element 11 passes through the optical element group 13 and is focused on the center of the beam scanning device 30.
  • the beam scanning device 30 reflects the beam to the first lens 70, the beam passes through the first lens 70 and becomes a quasi-parallel beam, and the beam passes through the first sample to be measured in the accommodating cavity.
  • the light beam passes through the first sample to be tested and then reaches the second lens 80.
  • the second lens 80 is used to focus the light beam passing through the corresponding accommodating cavity onto the first end surface 41a.
  • the light beam passes through the multimode optical fiber 41 and exits from the second end surface 41b.
  • the entrance slit of the optical fiber spectrometer 42 coincides with the second end surface 41b, and the direction of the slit is the same as the direction in which the plurality of second end surfaces 41b are linearly arranged.
  • the measurement results are obtained through data analysis to obtain the first spectrum.
  • the optical fiber spectrometer 42 sends out a second trigger signal, which first triggers the optical scanning device 20 to reach the second preset angle.
  • the subsequent steps are the same as in the first measurement, and the second spectrum is finally obtained.
  • the spectrophotometer detection system disclosed in the embodiments of the present invention realizes rapid spectrophotometric measurement of multiple samples under the condition of a single light source and a single spectral module, eliminating the influence of light source intensity and output spectrum fluctuations on the spectrophotometer, and improving the detection accuracy of the system. In addition, it can make the structure more compact and easy to integrate, while reducing costs.
  • the liquid in-situ spectrophotometer detection system includes a housing 90 and the spectrophotometer detection system in the first or second embodiment, the spectrophotometer detection system is provided at In the housing 90, a portion of the housing 90 is recessed to form a liquid in-situ measurement window 91, and opposite sides of the liquid in-situ measurement window 91 are respectively provided with a first light-transmitting portion 92) and a second light-transmitting portion 93.
  • the beam scanning device 30 is also used to reflect the incident light beam through the first light-transmitting portion 92 and the second light-transmitting portion 93 in sequence, and the spectrum detection device 40 is also used to receive the second light-transmitting The light beam of the portion 93, and acquire the spectrum of the light beam of the second light transmitting portion 93.
  • the entire liquid in-situ spectrophotometer detection system is immersed in the liquid to be measured for measurement.
  • the light source device 10 is used to provide the incident light beam required for detection
  • the sample containing device 20 is a pure solvent containing cavity corresponding to the liquid to be measured, and its length is the same as the liquid in-situ measurement window 91.
  • the beam scanning device 30 is used to reflect the incident light beam to pass through the sample holding device 20 or the liquid in-situ measurement window 91
  • the spectrum detection device 40 is used to receive the light beam passing through the accommodating cavity and acquire the light beam passing through the accommodating cavity spectrum.
  • the beam scanning device 30 is further used to reflect the incident light beam through the sample holding device 20 or the liquid in-situ measurement window 91 in a time-sharing manner, so as to realize the in-situ reference measurement of the liquid to be measured.
  • the spectrophotometer detection system further includes a driving device 50 for driving the beam scanning device 30 to perform spatial scanning in a time-sharing manner, so that the beam scanning device 30 reflects the incident light beam in a time-sharing manner.
  • the light source device 10 includes a light emitting element 11 and a beam shaper 12, the light emitting element 11 is used to emit an incident light beam, and the beam shaper 12 is used to quasi-parallel shape the incident light beam emitted by the light emitting element 11.
  • the light-emitting element 11 preferentially uses a flashing xenon lamp.
  • the spectrophotometer detection system further includes two reflecting mirrors 60 for reflecting the light beam onto which the incident light beam is reflected by the beam scanning device 30 through the corresponding sample receiving device 20 or liquid in-situ measurement window 90.
  • both the surface of the reflecting mirror 60 and the reflecting surface of the beam scanning device 30 are coated with an ultraviolet enhanced reflecting film.
  • the incident light beam can pass through the sample receiving device 20 or the liquid in-situ measurement window 90 quasi-parallel.
  • the spectrum detection device 40 includes a Y-type multimode optical fiber 41 and an optical fiber spectrometer 42.
  • the spectrophotometer detection system further includes two second convex lenses 80 for focusing the light beam passing through the corresponding accommodating cavity to the end surface of the Y-shaped optical fiber 41.
  • the detection system of the spectrophotometer of the present invention controls the timing of the light-emitting element 11, the optical fiber spectrometer 42 and the optical scanning device 30 through the timing controller to realize time-sharing measurement.
  • the detection process of the spectrophotometer detection system is described below.
  • the optical fiber spectrometer 42 sends out a first trigger signal, which first triggers the optical scanning device 30 to reach the first preset angle. Then, the light-emitting element 11 is triggered to generate pulsed light, and the light beam emitted by the light-emitting element 11 becomes a quasi-parallel light after passing through the beam shaper 12. The quasi-parallel beam passes through the optical scanning device 30 and is reflected to the mirror 60. The reflecting mirror 60 directs the light beam to the sample containing device 20, and the light beam passes through the pure solvent of the liquid to be measured in the accommodating cavity.
  • the light beam passes through the sample receiving device 20 to the second convex lens 80, and is used to focus the light beam passing through the corresponding receiving cavity to the first end surface of the Y-shaped optical fiber.
  • the light beam passes through the multimode optical fiber 41 and exits from the second end surface.
  • the entrance slit of the optical fiber spectrometer 42 coincides with the second end surface of the Y-shaped optical fiber 41.
  • the measurement results are obtained through data analysis to obtain the first spectrum.
  • the optical fiber spectrometer 42 sends out a second trigger signal, which first triggers the optical scanning device 30 to reach the second preset angle.
  • the subsequent steps are the same as in the first measurement, and the second spectrum is finally obtained.
  • the second spectrum is subtracted from the first spectrum to obtain the in-situ absorption spectrum of the liquid to be measured.

Abstract

A spectrophotometer inspection system and method. The inspection system comprises: a light source device (10) for providing an incident light beam; a sample accommodation device (20) comprising multiple accommodation cavities where samples to be inspected are accommodated; a light beam scanning device (30) for reflecting the incident light beam and passing same through the accommodation cavity; and a spectrum detection device (40) for receiving the light beam passing through the accommodation cavity and obtaining the spectrum of the light beam passing through the accommodation cavity. According to the spectrophotometer inspection system, the rapid spectrophotometric measurement on multiple samples is achieved in a single light source and a single spectrum module, the influences of the light intensity and the fluctuation of an output spectrum on a spectrophotometer are eliminated, and the system detection precision is improved. In addition, the structure is made more compact for integration, and costs are reduced.

Description

分光光度计检测系统及其检测方法Spectrophotometer detection system and detection method 技术领域Technical field
本发明属于光学测量技术领域，特别涉及一种分光光度计检测系统及其检测方法。The invention belongs to the technical field of optical measurement, and particularly relates to a spectrophotometer detection system and a detection method thereof.
背景技术Background technique
分光光度计利用物质的吸收光谱来推断物质成分的种类和含量。分光光度法的基本定律是朗伯-比尔定律(Lambert-Beer law)，它是描述物质对某一波长光吸收的强弱与吸光物质的浓度及其液层厚度间的关系。具体公式为：A＝Lg(I/I 0)＝A×b×c，其中A为吸光度，I为透过光强度，I 0为入射光强度，b为吸收路径的长度，c为吸光物质的浓度。 The spectrophotometer uses the absorption spectrum of the substance to infer the type and content of the substance component. The basic law of spectrophotometry is Lambert-Beer law (Lambert-Beer law), which describes the relationship between the intensity of a substance's absorption of light at a certain wavelength and the concentration of the light-absorbing substance and the thickness of the liquid layer. The specific formula is: A=Lg(I/I 0 )=A×b×c, where A is the absorbance, I is the transmitted light intensity, I 0 is the incident light intensity, b is the length of the absorption path, and c is the light-absorbing substance concentration.
分光光度计常常采用氙灯、氘灯、卤钨灯等作为光源，这些光源都有着相对较宽的光谱可以覆盖到多个波段。这类非相干光源在工作时具有一定的强度波动，它影响了传统结构的吸收光谱仪的稳定性。再则，这些光源的光谱输出特性不够稳定，即在不同波长的输出会出现比例差异。这种光源能量输出以及光谱输出的不稳定性会给分时测量的光谱引入误差。科学仪器检测中常用加入参比光路的方法来消除光源能量输出以及光谱输出的不稳定性给测量结果带来的影响。参比光路与包含样品的检测光路相比，一般是不含任何溶质成分的溶剂。先测量包含样品的测试光路然后移动样品池测量参比光路。将朗伯-比尔定律应用在有参比的光谱测量中，表达式为：
Figure PCTCN2019125192-appb-000001
Abs λ表示在波长为λ位置的吸光度，I 0λ表示参比光路采集的光谱；I λ为信号光路采集的光谱。通过这种光谱相减的方式消除光源不稳定对实验结果的影响。 Spectrophotometers often use xenon lamps, deuterium lamps, tungsten halogen lamps, etc. as light sources. These light sources have a relatively wide spectrum that can cover multiple wavelength bands. This type of incoherent light source has a certain intensity fluctuation during operation, which affects the stability of the absorption spectrometer of traditional structure. Furthermore, the spectral output characteristics of these light sources are not stable enough, that is, there will be a proportional difference in the output at different wavelengths. The instability of the energy output of the light source and the spectral output will introduce errors into the time-sharing spectrum. The method of adding a reference optical path is commonly used in the detection of scientific instruments to eliminate the influence of the instability of the energy output of the light source and the spectral output on the measurement results. The reference optical path is generally a solvent that does not contain any solute components compared to the detection optical path containing the sample. Measure the test light path containing the sample first and then move the sample cell to measure the reference light path. Lambert-Beer law is applied to the reference spectrum measurement, the expression is:
Figure PCTCN2019125192-appb-000001
Abs λ represents the absorbance at the wavelength λ, I 0λ represents the spectrum collected by the reference optical path; I λ represents the spectrum collected by the signal optical path. By this way of spectral subtraction, the influence of light source instability on the experimental results is eliminated.
常见的参比光路的实现方式有两种，第一是依靠样品池的位置切换来实现参比光路与样品光路的切换。这种方式由于机械的移动精度造成复位的时候存在位置偏差，降低了系统的测量精度；第二是依靠多个光源或者光谱检测模块来实现参比光路与样品光路的同时采集。这种方式会带入新的误差，降低测量精度，如不同光源的特性差异、不同光谱检测模块的光谱响应差异。另外，双光源或双光谱检测模块也大大提高了分光光度计检测系统的成本。There are two common ways of implementing the reference optical path. The first is to switch the reference optical path and the sample optical path by switching the position of the sample cell. In this way, there is a position deviation when resetting due to the movement accuracy of the machine, which reduces the measurement accuracy of the system. The second is to rely on multiple light sources or spectral detection modules to achieve the simultaneous acquisition of the reference optical path and the sample optical path. This approach will introduce new errors and reduce measurement accuracy, such as differences in the characteristics of different light sources and differences in the spectral response of different spectral detection modules. In addition, the dual light source or dual spectrum detection module also greatly increases the cost of the spectrophotometer detection system.
测量时间对于分光光度计是一个重要的参数，首先单次测量时间决定了分光光度计是否可以应用于原位测量，比如水质参数(化学需氧量、总有机碳等)原位测量中，如果可以满足测量时间在毫秒量级完成，就几乎可以忽略由于水流带来的水体成分变化。再则，在有参比光路的分光光度计中，测量时间还决定了参比光路和样品光路在先后测量中，光源的不稳定性对两次测量结果的影响，最终影响探测精度。综上，测量快速是分光光度计的核心特性之一。The measurement time is an important parameter for the spectrophotometer. First, the single measurement time determines whether the spectrophotometer can be used for in-situ measurement, such as in-situ measurement of water quality parameters (chemical oxygen demand, total organic carbon, etc.). It can be satisfied that the measurement time is completed on the order of milliseconds, and the change of the water composition caused by the water flow can be almost ignored. Furthermore, in a spectrophotometer with a reference optical path, the measurement time also determines the reference optical path and the sample optical path in the successive measurement. The influence of the instability of the light source on the two measurement results ultimately affects the detection accuracy. In summary, rapid measurement is one of the core features of spectrophotometers.
现有技术中，专利申请公开号为CN107941717A，发明名称为一种静态多样品池分光光度计的中国专利中，其采用一字排开的线性结构，然后依靠步进电机把多个样品池移动到光路位置上进行测量。整个过程样品通道越多耗时越长，“5连池”结构的一次测量要在分钟量级，并且占用了大量的空间，使系统很难小型化。In the prior art, the patent application publication number is CN107941717A, and the Chinese patent of the invention titled a static multi-sample cell spectrophotometer uses a linear structure lined up, and then relies on a stepper motor to move multiple sample cells Measure at the optical path. The more sample channels in the whole process, the longer it takes. The measurement of the "5-connected cell" structure must be in the order of minutes, and it takes up a lot of space, making it difficult to miniaturize the system.
发明内容Summary of the invention
(一)本发明所要解决的技术问题(1) Technical problems to be solved by the present invention
本发明要解决的技术问题是：如何在单个光源单个光谱检测模块上实现多个待测样品的快速分光光度测量。The technical problem to be solved by the present invention is: how to realize rapid spectrophotometric measurement of multiple samples to be tested on a single light source and a single spectrum detection module.
(二)本发明所采用的技术方案(2) The technical solution adopted by the present invention
为了实现上述的目的，本发明采用了如下的技术方案：In order to achieve the above objectives, the present invention adopts the following technical solutions:
一种分光光度计检测系统，包括：A spectrophotometer detection system, including:
光源装置，用于提供入射光束；Light source device for providing incident light beam;
样品容纳装置，包括多个用于容纳待测样品的容置腔；The sample containing device includes a plurality of containing chambers for containing the sample to be tested;
光束扫描装置，用于将入射光束反射穿过所述容置腔；A beam scanning device for reflecting the incident beam through the accommodating cavity;
光谱探测装置，用于接收穿过所述容置腔的光束，并获取穿过所述容置腔的光束的光谱。The spectrum detection device is used for receiving the light beam passing through the accommodating cavity and acquiring the spectrum of the light beam passing through the accommodating cavity.
可选择地，所述分光光度计检测系统还包括：驱动装置，用于驱动所述光束扫描装置进行分时地空间扫描，以分时、分空间地将入射光束反射穿过各个容置腔。Optionally, the spectrophotometer detection system further includes: a driving device for driving the light beam scanning device to perform time-division spatial scanning to reflect the incident light beam through each containing cavity in a time-division and space-division manner.
可选择地，分光光度计检测系统还包括：多个反射镜，所述反射镜与所述容置腔一一对应，所述反射镜用于将所述光束扫描装置反射入射光束至其上的 光束反射穿过对应的所述容置腔。Optionally, the spectrophotometer detection system further includes: a plurality of reflecting mirrors, the reflecting mirrors correspond to the accommodating cavity in one-to-one correspondence, the reflecting mirrors are used to reflect the incident beam onto the beam scanning device The light beam reflects through the corresponding cavity.
可选择地，所述光源装置包括：Optionally, the light source device includes:
发光元件，用于发射入射光束；Light emitting element, used to emit incident light beam;
光束整形器，用于将所述发光元件发射的入射光束进行准平行整形。The beam shaper is used to quasi-parallel shape the incident light beam emitted by the light-emitting element.
可选择地，分光光度计检测系统还包括：具有正屈光力的第一透镜，所述第一透镜的焦点与所述光束扫描装置的中心重合，所述第一透镜用于将所述光束扫描装置分时地反射入射光束至其上的光束反射穿过各个容置腔。Optionally, the spectrophotometer detection system further includes: a first lens with positive refractive power, a focal point of the first lens coincides with the center of the beam scanning device, and the first lens is used to scan the beam scanning device The light beam reflected onto the incident light beam by time-sharing reflects through each accommodating cavity.
可选择地，所述光源装置包括：Optionally, the light source device includes:
发光元件，用于发射入射光束；Light emitting element, used to emit incident light beam;
具有正屈光力的光学元件组，用于将所述发光元件发射的入射光束聚焦到所述光束扫描装置的中心上。An optical element group with positive refractive power is used to focus the incident light beam emitted by the light-emitting element onto the center of the light beam scanning device.
可选择地，所述样品容纳装置包括容纳盘、第一窗盖和第二窗盖，所述容纳盘中设置有多个贯穿所述容纳盘的相对的第一表面和第二表面的通孔，所述第一窗盖设置于所述第一表面上，所述第二窗盖设置于所述第二表面上，从而形成多个所述容置腔。Optionally, the sample receiving device includes a receiving tray, a first window cover, and a second window cover, and the receiving tray is provided with a plurality of through holes penetrating the first and second opposing surfaces of the receiving tray , The first window cover is disposed on the first surface, and the second window cover is disposed on the second surface, thereby forming a plurality of the receiving chambers.
可选择地，所述光谱探测装置包括：Optionally, the spectrum detection device includes:
多条多模光纤，每条多模光纤包括相对的第一端面和第二端面，所述多条多模光纤的第二端面呈线性排列，所述第一端面和所述容置腔一一对应，所述第一端面用于接收穿过对应的所述容置腔的光束；Multiple multimode optical fibers, each multimode optical fiber includes opposing first end surfaces and second end surfaces, the second end surfaces of the multiple multimode optical fibers are linearly arranged, and the first end surface and the receiving cavity are one by one Correspondingly, the first end surface is used to receive the light beam passing through the corresponding accommodating cavity;
光纤光谱仪，其入射狭缝与线性排列的多个第二端面对合，所述光纤光谱仪用于接收所述第二端面出射的光束。In the optical fiber spectrometer, the entrance slit meets a plurality of linearly arranged second end faces, and the optical fiber spectrometer is used to receive the light beam emitted from the second end face.
本发明还公开了一种液体原位分光光度计检测系统，包括外壳和上述的分光光度计检测系统，所述分光光度计检测系统设置于外壳内，所述外壳部分凹陷形成液体原位测量窗口，所述液体原位测量窗口的相对两侧分别设置有第一透光部和第二透光部，所述光束扫描装置还用于将入射光束反射依次穿过所述第一透光部和所述第二透光部，所述光谱探测装置还用于接收穿过所述第二透光部的光束，并获取所述第二透光部的光束的光谱。The invention also discloses a liquid in-situ spectrophotometer detection system, including a casing and the above spectrophotometer detection system, the spectrophotometer detection system is provided in the casing, and the casing is partially recessed to form a liquid in-situ measurement window , Opposite sides of the liquid in-situ measurement window are respectively provided with a first light-transmitting portion and a second light-transmitting portion, and the beam scanning device is further used to reflect incident light beams through the first light-transmitting portion and In the second light-transmitting part, the spectrum detection device is further used to receive the light beam passing through the second light-transmitting part and acquire the spectrum of the light beam in the second light-transmitting part.
本发明还公开了一种分光光度计检测系统的检测方法，所述检测方法包括：The invention also discloses a detection method of the spectrophotometer detection system. The detection method includes:
光源装置提供入射光束；The light source device provides the incident beam;
光束扫描装置将入射光束反射穿过样品容纳装置的容置腔，所述样品容纳装置的容置腔中包含待测样品以及参比样品；The beam scanning device reflects the incident light beam through the accommodating cavity of the sample accommodating device, and the accommodating cavity of the sample accommodating device contains the sample to be tested and the reference sample;
光谱探测装置接收穿过所述容置腔的光束，并获取穿过所述容置腔的光束的光谱，其中，所述光谱包括待测样品的光谱以及所述参比样品的光谱；The spectrum detection device receives the light beam passing through the accommodating cavity, and acquires the spectrum of the light beam passing through the accommodating cavity, where the spectrum includes the spectrum of the sample to be tested and the spectrum of the reference sample;
根据待测样品的光谱以及所述参比样品的光谱得到待测样品的吸收光谱。The absorption spectrum of the sample to be measured is obtained according to the spectrum of the sample to be measured and the spectrum of the reference sample.
(三)有益效果(3) Beneficial effects
本发明公开的分光光度计检测系统在单个光源单个光谱模块的条件下实现多个样品的快速分光光度测量，消除光源强度和输出光谱波动对分光光度计的影响，提高系统探测精度，另外可以使结构更加紧凑便于集成，同时降低了成本。The spectrophotometer detection system disclosed by the invention realizes the rapid spectrophotometric measurement of multiple samples under the condition of a single light source and a single spectral module, eliminates the influence of light source intensity and output spectrum fluctuations on the spectrophotometer, improves the detection accuracy of the system, and can also make The structure is more compact and easy to integrate, while reducing costs.
附图说明BRIEF DESCRIPTION
图1是本发明的实施例的分光光度计检测系统的检测方法的流程图；1 is a flowchart of a detection method of a spectrophotometer detection system according to an embodiment of the present invention;
图2是本发明的实施例一的分光光度计检测系统的示意图；2 is a schematic diagram of a spectrophotometer detection system according to Embodiment 1 of the present invention;
图3是本发明的实施例一的样品容纳装置结构分解图；FIG. 3 is an exploded view of the structure of the sample holding device according to the first embodiment of the present invention;
图4是本发明的实施例一的多条多模光纤的第一端面排列示意图；4 is a schematic diagram of the arrangement of the first end faces of multiple multimode optical fibers according to Embodiment 1 of the present invention;
图5是本发明的实施例一的多条多模光纤的第二端面排列示意图；5 is a schematic diagram of the arrangement of second end faces of multiple multimode optical fibers according to Embodiment 1 of the present invention;
图6是本发明的实施例二的分光光度计检测系统的示意图；6 is a schematic diagram of a spectrophotometer detection system according to Embodiment 2 of the present invention;
图7是本发明的实施例三的液体原位分光光度计检测系统的示意图。7 is a schematic diagram of a liquid in-situ spectrophotometer detection system according to Embodiment 3 of the present invention.
具体实施方式detailed description
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合附图及实施例，对本发明进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not intended to limit the present invention.
如图1所示，根据本发明的实施例的分光光度计检测系统的检测方法包括如下步骤S10至步骤S30：As shown in FIG. 1, the detection method of the spectrophotometer detection system according to the embodiment of the present invention includes the following steps S10 to S30:
步骤S10：光源装置10提供入射光束；Step S10: the light source device 10 provides an incident light beam;
步骤S20：光束扫描装置30将入射光束反射穿过样品容纳装置20的容置腔，其中，所述样品容纳装置(20)的容置腔中包含待测样品以及参比样品；Step S20: The beam scanning device 30 reflects the incident beam through the accommodating cavity of the sample accommodating device 20, wherein the accommodating cavity of the sample accommodating device (20) contains the sample to be tested and the reference sample;
步骤S30：光谱探测装置40接收穿过容置腔的光束，并获取穿过所述容置腔的光束的光谱，其中，所述光谱包括待测样品的光谱以及所述参比样品的光谱。Step S30: The spectrum detection device 40 receives the light beam passing through the accommodating cavity and acquires the spectrum of the light beam passing through the accommodating cavity, where the spectrum includes the spectrum of the sample to be tested and the spectrum of the reference sample.
步骤S40：根据待测样品的光谱以及所述参比样品的光谱得到待测样品的吸收光谱。Step S40: Obtain the absorption spectrum of the sample to be tested according to the spectrum of the sample to be tested and the spectrum of the reference sample.
进一步地，下面通过两个不同的实施例来说明如何利用分光光度计检测系统进行测量。Further, the following uses two different embodiments to explain how to use the spectrophotometer detection system for measurement.
实施例一Example one
如图2所示，根据本发明的实施例一的分光光度计检测系统包括光源装置10、样品容纳装置20、光束扫描装置30和光谱探测装置40。其中，光源装置10用于提供探测所需的入射光束，样品容纳装置20包括多个用于容纳待测样品的容置腔，光束扫描装置30用于将入射光束反射以穿过容置腔，光谱探测装置40用于接收穿过容置腔的光束，并获取穿过容置腔的光束的光谱。As shown in FIG. 2, the spectrophotometer detection system according to Embodiment 1 of the present invention includes a light source device 10, a sample holding device 20, a beam scanning device 30 and a spectrum detection device 40. Among them, the light source device 10 is used to provide the incident light beam required for detection, the sample receiving device 20 includes a plurality of receiving cavities for receiving the sample to be tested, and the beam scanning device 30 is used to reflect the incident light beam to pass through the receiving cavity, The spectrum detecting device 40 is used to receive the light beam passing through the accommodating cavity and acquire the spectrum of the light beam passing through the accommodating cavity.
具体地，光束扫描装置30进一步用于分时地将入射光束反射穿过各个容置腔，以实现各个待测样品的测量。作为优选实施例，分光光度计检测系统还包括驱动装置50，驱动装置50用于分时地驱动光束扫描装置30进行转动，以使光束扫描装置30分时地反射入射光束。Specifically, the beam scanning device 30 is further used to reflect the incident light beam through each receiving cavity in a time-sharing manner, so as to realize the measurement of each sample to be measured. As a preferred embodiment, the spectrophotometer detection system further includes a driving device 50 for driving the light beam scanning device 30 to rotate in a time-sharing manner, so that the light beam scanning device 30 reflects the incident light beam in a time-sharing manner.
进一步地，作为优选实施例，如图3所示，样品容纳装置20包括容纳盘21、第一窗盖22和第二窗盖23，容纳盘21中设置有多个贯穿容纳盘21的相对的第一表面和第二表面的通孔21a，第一窗盖22设置于第一表面上，第二窗盖22设置于第二表面上，从而形成多个容置腔。进一步地，多个通孔21a呈阵列排布，形成蜂窝状结构。通孔21a的横截面形状为正六边形，容纳盘21的形状为圆柱体形。容纳盘21的厚度优选为10mm，通孔21a的外接圆直径为5mm，容纳盘21的材料采用普通玻璃材料，第一窗盖22和第二窗盖23的材料优选为紫外熔融石英材料制成。进一步地，第一窗盖22和第二窗盖23均采用可拆卸设计，装样的时候将第一窗盖22打开，将不同的液体样品注满各个通孔21a中，然后将第一窗盖22盖好，最后将样品容纳装置20放回系统中进 行测量，清洗的时候同时将第一窗盖22和第二窗盖23取下清洗。Further, as a preferred embodiment, as shown in FIG. 3, the sample receiving device 20 includes a receiving tray 21, a first window cover 22 and a second window cover 23, and a plurality of opposing In the through holes 21a of the first surface and the second surface, the first window cover 22 is disposed on the first surface, and the second window cover 22 is disposed on the second surface, thereby forming a plurality of accommodating cavities. Further, the plurality of through holes 21a are arranged in an array to form a honeycomb structure. The cross-sectional shape of the through hole 21a is a regular hexagon, and the shape of the accommodating disk 21 is a cylindrical shape. The thickness of the accommodating tray 21 is preferably 10 mm, the diameter of the circumscribed circle of the through hole 21 a is 5 mm, the material of the accommodating tray 21 is made of ordinary glass, and the material of the first window cover 22 and the second window cover 23 is preferably made of ultraviolet fused silica material . Further, both the first window cover 22 and the second window cover 23 adopt a detachable design. When loading the sample, the first window cover 22 is opened, different liquid samples are filled into the through holes 21a, and then the first window The cover 22 is closed, and finally the sample holding device 20 is put back into the system for measurement, and the first window cover 22 and the second window cover 23 are removed for cleaning at the same time when cleaning.
进一步地，光源装置10包括发光元件11和光束整形器12，发光元件11用于发射入射光束，光束整形器12用于将发光元件11发射的入射光束进行准平行整形。其中发光元件11优先采用闪烁氙灯。进一步地，分光光度计检测系统还包括多个反射镜60，反射镜60与容置腔一一对应，反射镜60用于将光束扫描装置30反射入射光束至其上的光束反射穿过对应的容置腔。其中，反射镜60表面和光束扫描装置30的反射表面均镀有紫外增强反射膜。经过上述反射镜60和光束扫描装置30的作用，这样可使得入射光束准平行地穿过容置腔。Further, the light source device 10 includes a light emitting element 11 and a beam shaper 12, the light emitting element 11 is used to emit an incident light beam, and the beam shaper 12 is used to quasi-parallel shape the incident light beam emitted by the light emitting element 11. The light-emitting element 11 preferentially uses a flashing xenon lamp. Further, the spectrophotometer detection system further includes a plurality of reflecting mirrors 60, the reflecting mirrors 60 correspond to the accommodating cavities one by one, and the reflecting mirrors 60 are used to reflect the incident beams reflected by the beam scanning device 30 to the beams passing thereon through the corresponding The cavity is accommodated. Wherein, both the surface of the reflecting mirror 60 and the reflecting surface of the beam scanning device 30 are coated with an ultraviolet enhanced reflecting film. Through the function of the reflecting mirror 60 and the beam scanning device 30, the incident beam can pass through the accommodating cavity quasi-parallel.
作为优选实施例，光谱探测装置40包括多条多模光纤41和光纤光谱仪42，如图4和图5所示，每条多模光纤41包括相对的第一端面41a和第二端面41b，多条多模光纤41的第二端面41b呈线性排列，第一端面41a和容置腔一一对应，第一端面41a用于接收穿过对应的容置腔的光束。光纤光谱仪42的入射狭缝与线性排列的多个第二端面41b对合，光纤光谱仪42用于接收第二端面41b出射的光束。As a preferred embodiment, the spectrum detection device 40 includes a plurality of multimode optical fibers 41 and a fiber spectrometer 42, as shown in FIGS. 4 and 5, each multimode optical fiber 41 includes opposing first end surfaces 41a and second end surfaces 41b. The second end surfaces 41b of the multi-mode optical fibers 41 are arranged linearly. The first end surfaces 41a and the accommodating cavities are in one-to-one correspondence. The first end surface 41a is used to receive the light beam passing through the corresponding accommodating cavities. The entrance slit of the optical fiber spectrometer 42 is aligned with the plurality of linearly arranged second end faces 41b. The optical fiber spectrometer 42 is used to receive the light beam emitted from the second end face 41b.
进一步地，分光光度计检测系统还包括第二透镜80，第二透镜80用于将穿过对应的容置腔的光束聚焦至第一端面41a上，且第一端面41a位于第二透镜80的焦点处，第一端面41a与穿过对应的容置腔的光束的光轴垂直。其中，第二透镜80的直径大于容纳盘21的外直径。Further, the spectrophotometer detection system further includes a second lens 80 for focusing the light beam passing through the corresponding accommodating cavity onto the first end surface 41a, and the first end surface 41a is located on the second lens 80 At the focal point, the first end surface 41a is perpendicular to the optical axis of the light beam passing through the corresponding receiving cavity. Among them, the diameter of the second lens 80 is larger than the outer diameter of the accommodating disk 21.
进一步地，本发明的分光光度计检测系统通过时序控制器来控制发光元件11、光纤光谱仪42和光学扫描装置20的时序，以实现分时测量。下面描述分光光度计检测系统的检测过程。Further, the detection system of the spectrophotometer of the present invention controls the timing of the light-emitting element 11, the optical fiber spectrometer 42 and the optical scanning device 20 through a timing controller to realize time-sharing measurement. The detection process of the spectrophotometer detection system is described below.
第一次测量：光纤光谱仪42发出第一触发信号，首先触发光学扫描装置20到达第一预设角度。接着触发发光元件11产生脉冲光，发光元件11发出的光束经过光束整形器12后变成准平行光。准平行光束经过光学扫描装置20后被反射到反射镜60。反射镜60将光束中至容置腔，光束穿过容置腔中的第一待测样品。光束穿过第一待测样品后到第二透镜80，第二透镜80用于将穿过对应的容置腔的光束聚焦至第一端面41a上。光束在多模光纤41中穿过，从第二端面41b出射。光纤光谱仪42的入射狭缝与第二端面41b的重合，并且狭缝方向与多个第二端面41b线性排列的方向相同。最后经过数据分析获得测 量结果，得到第一光谱。第二测量时，光纤光谱仪42发出第二触发信号，首先触发光学扫描装置20到达第二预设角度，后续步骤跟第一次测量时相同，最终得到第二光谱。依次类推，经过多次测量后可完成多个待测样品的测试，其中多个待测样品的其中一个样品为参比样品。First measurement: The optical fiber spectrometer 42 sends out a first trigger signal, which first triggers the optical scanning device 20 to reach the first preset angle. Then, the light-emitting element 11 is triggered to generate pulsed light, and the light beam emitted by the light-emitting element 11 becomes a quasi-parallel light after passing through the beam shaper 12. The quasi-parallel beam passes through the optical scanning device 20 and is reflected to the mirror 60. The mirror 60 directs the light beam to the containing cavity, and the light beam passes through the first sample to be measured in the containing cavity. The light beam passes through the first sample to be tested and then reaches the second lens 80. The second lens 80 is used to focus the light beam passing through the corresponding accommodating cavity onto the first end surface 41a. The light beam passes through the multimode optical fiber 41 and exits from the second end surface 41b. The entrance slit of the optical fiber spectrometer 42 coincides with the second end surface 41b, and the direction of the slit is the same as the direction in which the plurality of second end surfaces 41b are linearly arranged. Finally, the measurement results are obtained through data analysis to obtain the first spectrum. In the second measurement, the optical fiber spectrometer 42 sends out a second trigger signal, which first triggers the optical scanning device 20 to reach the second preset angle. The subsequent steps are the same as in the first measurement, and the second spectrum is finally obtained. By analogy, after multiple measurements, the test of multiple test samples can be completed, and one of the multiple test samples is the reference sample.
实施例二Example 2
当然在其他实施方式中，如图6所示，光源装置10包括发光元件11和具有正屈光力的光学元件组13，光学元件组13可以为透镜组或凹面反射镜组，发光元件11用于发射入射光束，光学元件组13用于将发光元件11发射的入射光束聚焦到光束扫描装置30的中心上。进一步地，分光光度计检测系统还包括具有正屈光力的第一透镜70，第一透镜70的焦点与光束扫描装置30的中心重合，第一透镜70用于将光束扫描装置30分时地反射入射光束至其上的光束反射穿过各个容置腔。这样可使得入射光束准平行地穿过容置腔。本实施例二的其他部分与实施例一的内容相同，在此不进行赘述。Of course, in other embodiments, as shown in FIG. 6, the light source device 10 includes a light-emitting element 11 and an optical element group 13 with positive refractive power. The optical element group 13 may be a lens group or a concave mirror group, and the light-emitting element 11 is used to emit For the incident light beam, the optical element group 13 is used to focus the incident light beam emitted by the light-emitting element 11 onto the center of the beam scanning device 30. Further, the spectrophotometer detection system further includes a first lens 70 having positive refractive power, the focal point of the first lens 70 coincides with the center of the beam scanning device 30, and the first lens 70 is used to reflect the beam scanning device 30 in a time-sharing manner. The light beam onto which the light beam is reflected reflects through each accommodating cavity. This allows the incident beam to pass through the receiving cavity quasi-parallel. The other parts of the second embodiment are the same as those of the first embodiment, and will not be repeated here.
在本实施例二中，分光光度计检测系统的检测过程还可以是：In the second embodiment, the detection process of the spectrophotometer detection system may also be:
第一次测量：光纤光谱仪42发出第一触发信号，首先触发光学扫描装置20到达第一预设角度。接着触发发光元件11产生脉冲光，发光元件11发出的光束经过光学元件组13后被聚焦到光束扫描装置30的中心上。光束扫描装置30将光束反射至第一透镜70，光束经过第一透镜70变成准平行光束，光束穿过容置腔中的第一待测样品。光束穿过第一待测样品后到第二透镜80，第二透镜80用于将穿过对应的容置腔的光束聚焦至第一端面41a上。光束在多模光纤41中穿过，从第二端面41b出射。光纤光谱仪42的入射狭缝与第二端面41b的重合，并且狭缝方向与多个第二端面41b线性排列的方向相同。最后经过数据分析获得测量结果，得到第一光谱。第二测量时，光纤光谱仪42发出第二触发信号，首先触发光学扫描装置20到达第二预设角度，后续步骤跟第一次测量时相同，最终得到第二光谱。依次类推，经过多次测量后可完成多个待测样品的测试，其中多个待测样品的其中一个样品为参比样品。First measurement: The optical fiber spectrometer 42 sends out a first trigger signal, which first triggers the optical scanning device 20 to reach the first preset angle. Then, the light-emitting element 11 is triggered to generate pulsed light. The light beam emitted by the light-emitting element 11 passes through the optical element group 13 and is focused on the center of the beam scanning device 30. The beam scanning device 30 reflects the beam to the first lens 70, the beam passes through the first lens 70 and becomes a quasi-parallel beam, and the beam passes through the first sample to be measured in the accommodating cavity. The light beam passes through the first sample to be tested and then reaches the second lens 80. The second lens 80 is used to focus the light beam passing through the corresponding accommodating cavity onto the first end surface 41a. The light beam passes through the multimode optical fiber 41 and exits from the second end surface 41b. The entrance slit of the optical fiber spectrometer 42 coincides with the second end surface 41b, and the direction of the slit is the same as the direction in which the plurality of second end surfaces 41b are linearly arranged. Finally, the measurement results are obtained through data analysis to obtain the first spectrum. In the second measurement, the optical fiber spectrometer 42 sends out a second trigger signal, which first triggers the optical scanning device 20 to reach the second preset angle. The subsequent steps are the same as in the first measurement, and the second spectrum is finally obtained. By analogy, after multiple measurements, the test of multiple test samples can be completed, and one of the multiple test samples is the reference sample.
本发明的实施例公开的分光光度计检测系统在单个光源单个光谱模块的条件下实现多个样品的快速分光光度测量，消除光源强度和输出光谱波动对分光光度计的影响，提高系统探测精度，另外可以使结构更加紧凑便于集成，同时降低了成本。The spectrophotometer detection system disclosed in the embodiments of the present invention realizes rapid spectrophotometric measurement of multiple samples under the condition of a single light source and a single spectral module, eliminating the influence of light source intensity and output spectrum fluctuations on the spectrophotometer, and improving the detection accuracy of the system. In addition, it can make the structure more compact and easy to integrate, while reducing costs.
实施例三Example Three
如图7所示，根据本发明的实施例三的液体原位分光光度计检测系统包括外壳90和实施例一或实施例二中的分光光度计检测系统，所述分光光度计检测系统设置于外壳90内，所述外壳90部分凹陷形成液体原位测量窗口91，所述液体原位测量窗口91的相对两侧分别设置有第一透光部92)和第二透光部93，所述光束扫描装置30还用于将入射光束反射依次穿过所述第一透光部92和所述第二透光部93，所述光谱探测装置40还用于接收穿过所述第二透光部93的光束，并获取所述第二透光部93的光束的光谱。As shown in FIG. 7, the liquid in-situ spectrophotometer detection system according to the third embodiment of the present invention includes a housing 90 and the spectrophotometer detection system in the first or second embodiment, the spectrophotometer detection system is provided at In the housing 90, a portion of the housing 90 is recessed to form a liquid in-situ measurement window 91, and opposite sides of the liquid in-situ measurement window 91 are respectively provided with a first light-transmitting portion 92) and a second light-transmitting portion 93. The beam scanning device 30 is also used to reflect the incident light beam through the first light-transmitting portion 92 and the second light-transmitting portion 93 in sequence, and the spectrum detection device 40 is also used to receive the second light-transmitting The light beam of the portion 93, and acquire the spectrum of the light beam of the second light transmitting portion 93.
整个液体原位分光光度计检测系统浸没在待测液体之中进行测量。其中，光源装置10用于提供探测所需的入射光束，样品容纳装置20为与待测液体对应的纯溶剂容置腔，其长度与液体原位测量窗口91相同。光束扫描装置30用于将入射光束反射以穿过样品容纳装置20或者液体原位测量窗口91，光谱探测装置40用于接收穿过容置腔的光束，并获取穿过容置腔的光束的光谱。The entire liquid in-situ spectrophotometer detection system is immersed in the liquid to be measured for measurement. Wherein, the light source device 10 is used to provide the incident light beam required for detection, and the sample containing device 20 is a pure solvent containing cavity corresponding to the liquid to be measured, and its length is the same as the liquid in-situ measurement window 91. The beam scanning device 30 is used to reflect the incident light beam to pass through the sample holding device 20 or the liquid in-situ measurement window 91, and the spectrum detection device 40 is used to receive the light beam passing through the accommodating cavity and acquire the light beam passing through the accommodating cavity spectrum.
具体地，光束扫描装置30进一步用于分时地将入射光束反射穿过样品容纳装置20或者液体原位测量窗口91，以实现待测液体的原位参比测量。Specifically, the beam scanning device 30 is further used to reflect the incident light beam through the sample holding device 20 or the liquid in-situ measurement window 91 in a time-sharing manner, so as to realize the in-situ reference measurement of the liquid to be measured.
作为优选实施例，分光光度计检测系统还包括驱动装置50，驱动装置50用于分时地驱动光束扫描装置30进行空间扫描，以使光束扫描装置30分时地反射入射光束。As a preferred embodiment, the spectrophotometer detection system further includes a driving device 50 for driving the beam scanning device 30 to perform spatial scanning in a time-sharing manner, so that the beam scanning device 30 reflects the incident light beam in a time-sharing manner.
进一步地，光源装置10包括发光元件11和光束整形器12，发光元件11用于发射入射光束，光束整形器12用于将发光元件11发射的入射光束进行准平行整形。其中发光元件11优先采用闪烁氙灯。Further, the light source device 10 includes a light emitting element 11 and a beam shaper 12, the light emitting element 11 is used to emit an incident light beam, and the beam shaper 12 is used to quasi-parallel shape the incident light beam emitted by the light emitting element 11. The light-emitting element 11 preferentially uses a flashing xenon lamp.
进一步地，分光光度计检测系统还包括两个反射镜60，用于将光束扫描装置30反射入射光束至其上的光束反射穿过对应的样品容纳装置20或者液体原位测量窗口90。其中，反射镜60表面和光束扫描装置30的反射表面均镀有紫外增强反射膜。经过上述反射镜60和光束扫描装置30的作用，这样可使得入射光束准平行地穿过样品容纳装置20或者液体原位测量窗口90。Further, the spectrophotometer detection system further includes two reflecting mirrors 60 for reflecting the light beam onto which the incident light beam is reflected by the beam scanning device 30 through the corresponding sample receiving device 20 or liquid in-situ measurement window 90. Wherein, both the surface of the reflecting mirror 60 and the reflecting surface of the beam scanning device 30 are coated with an ultraviolet enhanced reflecting film. Through the action of the mirror 60 and the beam scanning device 30, the incident light beam can pass through the sample receiving device 20 or the liquid in-situ measurement window 90 quasi-parallel.
作为优选实施例，光谱探测装置40包括Y型多模光纤41和光纤光谱仪42。As a preferred embodiment, the spectrum detection device 40 includes a Y-type multimode optical fiber 41 and an optical fiber spectrometer 42.
进一步地，分光光度计检测系统还包括两个第二凸透镜80，用于将穿过对 应的容置腔的光束聚焦至Y型光纤41端面上。Further, the spectrophotometer detection system further includes two second convex lenses 80 for focusing the light beam passing through the corresponding accommodating cavity to the end surface of the Y-shaped optical fiber 41.
进一步地，本发明的分光光度计检测系统通过时序控制器来控制发光元件11、光纤光谱仪42和光学扫描装置30的时序，以实现分时测量。下面描述分光光度计检测系统的检测过程。Further, the detection system of the spectrophotometer of the present invention controls the timing of the light-emitting element 11, the optical fiber spectrometer 42 and the optical scanning device 30 through the timing controller to realize time-sharing measurement. The detection process of the spectrophotometer detection system is described below.
参比测量：光纤光谱仪42发出第一触发信号，首先触发光学扫描装置30到达第一预设角度。接着触发发光元件11产生脉冲光，发光元件11发出的光束经过光束整形器12后变成准平行光。准平行光束经过光学扫描装置30后被反射到反射镜60。反射镜60将光束中至样品容纳装置20，光束穿过容置腔中的待测液体的纯溶剂。光束穿过样品容纳装置20后到第二凸透镜80，用于将穿过对应的容置腔的光束聚焦至Y型光纤的第一端面上。光束在多模光纤41中穿过，从第二端面出射。光纤光谱仪42的入射狭缝与Y型光纤41的第二端面重合。最后经过数据分析获得测量结果，得到第一光谱。待测液体原位测量时，光纤光谱仪42发出第二触发信号，首先触发光学扫描装置30到达第二预设角度，后续步骤跟第一次测量时相同，最终得到第二光谱。将第二光谱与第一光谱相减得到待测液体的原位吸收光谱。Reference measurement: The optical fiber spectrometer 42 sends out a first trigger signal, which first triggers the optical scanning device 30 to reach the first preset angle. Then, the light-emitting element 11 is triggered to generate pulsed light, and the light beam emitted by the light-emitting element 11 becomes a quasi-parallel light after passing through the beam shaper 12. The quasi-parallel beam passes through the optical scanning device 30 and is reflected to the mirror 60. The reflecting mirror 60 directs the light beam to the sample containing device 20, and the light beam passes through the pure solvent of the liquid to be measured in the accommodating cavity. The light beam passes through the sample receiving device 20 to the second convex lens 80, and is used to focus the light beam passing through the corresponding receiving cavity to the first end surface of the Y-shaped optical fiber. The light beam passes through the multimode optical fiber 41 and exits from the second end surface. The entrance slit of the optical fiber spectrometer 42 coincides with the second end surface of the Y-shaped optical fiber 41. Finally, the measurement results are obtained through data analysis to obtain the first spectrum. During the in-situ measurement of the liquid to be measured, the optical fiber spectrometer 42 sends out a second trigger signal, which first triggers the optical scanning device 30 to reach the second preset angle. The subsequent steps are the same as in the first measurement, and the second spectrum is finally obtained. The second spectrum is subtracted from the first spectrum to obtain the in-situ absorption spectrum of the liquid to be measured.
尽管上面对本发明说明性的具体实施方式进行了描述，以便于本技术领域的技术人员能够理解本发明，但是本发明不仅限于具体实施方式的范围，对本技术领域的普通技术人员而言，只要各种变化只要在所附的权利要求限定和确定的本发明精神和范围内，一切利用本发明构思的发明创造均在保护之列。Although the illustrative specific embodiments of the present invention have been described above so that those skilled in the art can understand the present invention, the present invention is not limited to the scope of the specific embodiments. For those of ordinary skill in the art, as long as each As long as such changes are within the spirit and scope of the present invention as defined and determined by the appended claims, all inventions using the concept of the present invention are included in the protection.

Claims (17)

  1. 一种分光光度计检测系统，其中，包括：A spectrophotometer detection system, which includes:
    光源装置，用于提供入射光束；Light source device for providing incident light beam;
    样品容纳装置，包括多个用于容纳待测样品的容置腔；The sample containing device includes a plurality of containing chambers for containing the sample to be tested;
    光束扫描装置，用于将入射光束反射穿过所述容置腔；A beam scanning device for reflecting the incident beam through the accommodating cavity;
    光谱探测装置，用于接收穿过所述容置腔的光束，并获取穿过所述容置腔的光束的光谱。The spectrum detection device is used for receiving the light beam passing through the accommodating cavity and acquiring the spectrum of the light beam passing through the accommodating cavity.
  2. 根据权利要求1所述的分光光度计检测系统，其中，所述分光光度计检测系统还包括：驱动装置，用于驱动所述光束扫描装置进行分时地空间扫描，以分时、分空间地将入射光束反射穿过各个容置腔。The spectrophotometer detection system according to claim 1, wherein the spectrophotometer detection system further comprises: a driving device for driving the light beam scanning device to perform space-time spatial scanning, so that Reflect the incident beam through each receiving cavity.
  3. 根据权利要求2所述的分光光度计检测系统，其中，分光光度计检测系统还包括：多个反射镜，所述反射镜与所述容置腔一一对应，所述反射镜用于将所述光束扫描装置反射入射光束至其上的光束反射穿过对应的所述容置腔。The spectrophotometer detection system according to claim 2, wherein the spectrophotometer detection system further comprises: a plurality of mirrors, the mirrors correspond to the accommodating cavity in one-to-one correspondence, the mirrors are used to The light beam scanning device reflects the incident light beam onto the light beam and reflects through the corresponding cavity.
  4. 根据权利要求3所述的分光光度计检测系统，其中，所述光源装置包括：The spectrophotometer detection system according to claim 3, wherein the light source device comprises:
    发光元件，用于发射入射光束；Light emitting element, used to emit incident light beam;
    光束整形器，用于将所述发光元件发射的入射光束进行准平行整形。The beam shaper is used to quasi-parallel shape the incident light beam emitted by the light-emitting element.
  5. 根据权利要求2所述的分光光度计检测系统，其中，分光光度计检测系统还包括：具有正屈光力的第一透镜，所述第一透镜的焦点与所述光束扫描装置的中心重合，所述第一透镜用于将所述光束扫描装置分时地反射入射光束至其上的光束反射穿过各个容置腔。The spectrophotometer detection system according to claim 2, wherein the spectrophotometer detection system further comprises: a first lens having a positive refractive power, the focal point of the first lens coincides with the center of the beam scanning device, the The first lens is used to reflect the incident light beam onto the light beam scanning device in a time-sharing manner, and to reflect the light beam therethrough through each accommodating cavity.
  6. 根据权利要求5所述的分光光度计检测系统，其中，所述光源装置包括：The spectrophotometer detection system according to claim 5, wherein the light source device comprises:
    发光元件，用于发射入射光束；Light emitting element, used to emit incident light beam;
    具有正屈光力的光学元件组，用于将所述发光元件发射的入射光束聚焦到所述光束扫描装置的中心上。An optical element group with positive refractive power is used to focus the incident light beam emitted by the light-emitting element onto the center of the light beam scanning device.
  7. 根据权利要求1所述的分光光度计检测系统，其中，所述样品容纳装置包括容纳盘、第一窗盖和第二窗盖，所述容纳盘中设置有多个贯穿所述容纳盘的相对的第一表面和第二表面的通孔，所述第一窗盖设置于所述第一表面上，所述第二窗盖设置于所述第二表面上，从而形成多个所述容置腔。The spectrophotometer detection system according to claim 1, wherein the sample receiving device includes a receiving tray, a first window cover, and a second window cover, and a plurality of opposing surfaces extending through the receiving tray are provided in the receiving tray Through holes of the first surface and the second surface, the first window cover is disposed on the first surface, and the second window cover is disposed on the second surface, thereby forming a plurality of the accommodations Cavity.
  8. 根据权利要求1所述的分光光度计检测系统，其中，所述光谱探测装置包括：The spectrophotometer detection system according to claim 1, wherein the spectrum detection device comprises:
    多条多模光纤，每条多模光纤包括相对的第一端面和第二端面，所述多条多模光纤的第二端面呈线性排列，所述第一端面和所述容置腔一一对应，所述第一端面用于接收穿过对应的所述容置腔的光束；Multiple multimode optical fibers, each multimode optical fiber includes opposing first end surfaces and second end surfaces, the second end surfaces of the multiple multimode optical fibers are linearly arranged, and the first end surface and the receiving cavity are one by one Correspondingly, the first end surface is used to receive the light beam passing through the corresponding accommodating cavity;
    光纤光谱仪，其入射狭缝与线性排列的多个第二端面对合，所述光纤光谱仪用于接收所述第二端面出射的光束。In the optical fiber spectrometer, the entrance slit meets a plurality of linearly arranged second end faces, and the optical fiber spectrometer is used to receive the light beam emitted from the second end face.
  9. 一种液体原位分光光度计检测系统，其中，包括外壳和权利要求1所述的分光光度计检测系统，所述分光光度计检测系统设置于外壳内，所述外壳部分凹陷形成液体原位测量窗口，所述液体原位测量窗口的相对两侧分别设置有第一透光部和第二透光部，所述光束扫描装置还用于将入射光束反射依次穿过所述第一透光部和所述第二透光部，所述光谱探测装置还用于接收穿过所述第二透光部的光束，并获取所述第二透光部的光束的光谱。A liquid in-situ spectrophotometer detection system, which includes a housing and the spectrophotometer detection system of claim 1, the spectrophotometer detection system is disposed in the housing, and the housing is partially recessed to form an in-situ liquid measurement A window, on opposite sides of the liquid in-situ measurement window are respectively provided with a first light-transmitting portion and a second light-transmitting portion, and the beam scanning device is further used to reflect the incident light beam through the first light-transmitting portion in sequence With the second light-transmitting part, the spectrum detection device is further used to receive the light beam passing through the second light-transmitting part and acquire the spectrum of the light beam of the second light-transmitting part.
  10. 根据权利要求9所述的液体原位分光光度计检测系统，其中，所述分光光度计检测系统还包括：驱动装置，用于驱动所述光束扫描装置进行分时地空间扫描，以分时、分空间地将入射光束反射穿过各个容置腔。The liquid in-situ spectrophotometer detection system according to claim 9, wherein the spectrophotometer detection system further comprises: a driving device for driving the light beam scanning device to perform space-time spatial scanning to time-sharing, The incident beam is reflected spatially through each receiving cavity.
  11. 根据权利要求9所述的液体原位分光光度计检测系统，其中，所述分光光度计检测系统还包括：多个反射镜，所述反射镜与所述容置腔一一对应，所述反射镜用于将所述光束扫描装置反射入射光束至其上的光束反射穿过对应的所述容置腔。The liquid in-situ spectrophotometer detection system according to claim 9, wherein the spectrophotometer detection system further comprises: a plurality of mirrors, the mirrors correspond to the accommodating cavity in one-to-one correspondence, the reflection The mirror is used to reflect the light beam reflected by the light beam scanning device to the light beam passing therethrough through the corresponding receiving cavity.
  12. 根据权利要求11所述的液体原位分光光度计检测系统，其中，所述光源装置包括：The liquid in-situ spectrophotometer detection system according to claim 11, wherein the light source device comprises:
    发光元件，用于发射入射光束；Light emitting element, used to emit incident light beam;
    光束整形器，用于将所述发光元件发射的入射光束进行准平行整形。The beam shaper is used to quasi-parallel shape the incident light beam emitted by the light-emitting element.
  13. 根据权利要求9所述的液体原位分光光度计检测系统，其中，所述分 光光度计检测系统还包括：具有正屈光力的第一透镜，所述第一透镜的焦点与所述光束扫描装置的中心重合，所述第一透镜用于将所述光束扫描装置分时地反射入射光束至其上的光束反射穿过各个容置腔。The liquid in-situ spectrophotometer detection system according to claim 9, wherein the spectrophotometer detection system further comprises: a first lens having positive refractive power, the focal point of the first lens and the beam scanning device The centers coincide, and the first lens is used to reflect the incident light beam onto the light beam scanning device in a time-sharing manner, and to reflect the light beam passing through each accommodating cavity.
  14. 根据权利要求13所述的液体原位分光光度计检测系统，其中，所述光源装置包括：The liquid in-situ spectrophotometer detection system according to claim 13, wherein the light source device comprises:
    发光元件，用于发射入射光束；Light emitting element, used to emit incident light beam;
    具有正屈光力的光学元件组，用于将所述发光元件发射的入射光束聚焦到所述光束扫描装置的中心上。An optical element group with positive refractive power is used to focus the incident light beam emitted by the light-emitting element onto the center of the light beam scanning device.
  15. 根据权利要求9所述的液体原位分光光度计检测系统，其中，所述样品容纳装置包括容纳盘、第一窗盖和第二窗盖，所述容纳盘中设置有多个贯穿所述容纳盘的相对的第一表面和第二表面的通孔，所述第一窗盖设置于所述第一表面上，所述第二窗盖设置于所述第二表面上，从而形成多个所述容置腔。The liquid in-situ spectrophotometer detection system according to claim 9, wherein the sample holding device includes a holding tray, a first window cover, and a second window cover, a plurality of through trays are provided in the holding tray Through holes of the first surface and the second surface of the disc, the first window cover is disposed on the first surface, and the second window cover is disposed on the second surface, thereby forming a plurality of述容置腔。 Retaining cavity.
  16. 根据权利要求9所述的液体原位分光光度计检测系统，其中，所述光谱探测装置包括：The liquid in-situ spectrophotometer detection system according to claim 9, wherein the spectrum detection device comprises:
    多条多模光纤，每条多模光纤包括相对的第一端面和第二端面，所述多条多模光纤的第二端面呈线性排列，所述第一端面和所述容置腔一一对应，所述第一端面用于接收穿过对应的所述容置腔的光束；Multiple multimode optical fibers, each multimode optical fiber includes opposing first end surfaces and second end surfaces, the second end surfaces of the multiple multimode optical fibers are linearly arranged, and the first end surface and the receiving cavity are one by one Correspondingly, the first end surface is used to receive the light beam passing through the corresponding accommodating cavity;
    光纤光谱仪，其入射狭缝与线性排列的多个第二端面对合，所述光纤光谱仪用于接收所述第二端面出射的光束。In the optical fiber spectrometer, the entrance slit meets a plurality of linearly arranged second end faces, and the optical fiber spectrometer is used to receive the light beam emitted from the second end face.
  17. 一种如权利要求1所述的分光光度计检测系统的检测方法，其中，所述检测方法包括：A detection method of a spectrophotometer detection system according to claim 1, wherein the detection method comprises:
    光源装置提供入射光束；The light source device provides the incident beam;
    光束扫描装置将入射光束反射穿过样品容纳装置的容置腔，其中，所述样品容纳装置的容置腔中包含待测样品以及参比样品；The beam scanning device reflects the incident beam through the accommodating cavity of the sample accommodating device, wherein the accommodating cavity of the sample accommodating device contains the sample to be tested and the reference sample;
    光谱探测装置接收穿过所述容置腔的光束，并获取穿过所述容置腔的光束的光谱，其中，所述光谱包括待测样品的光谱以及所述参比样品的光谱；The spectrum detection device receives the light beam passing through the accommodating cavity, and acquires the spectrum of the light beam passing through the accommodating cavity, where the spectrum includes the spectrum of the sample to be tested and the spectrum of the reference sample;
    根据待测样品的光谱以及所述参比样品的光谱得到待测样品的吸收光谱。The absorption spectrum of the sample to be measured is obtained according to the spectrum of the sample to be measured and the spectrum of the reference sample.
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