WO2020116909A1 - Puce de diagnostic d'allergie et procédé de fabrication d'une puce de diagnostic d'allergie - Google Patents

Puce de diagnostic d'allergie et procédé de fabrication d'une puce de diagnostic d'allergie Download PDF

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Publication number
WO2020116909A1
WO2020116909A1 PCT/KR2019/016954 KR2019016954W WO2020116909A1 WO 2020116909 A1 WO2020116909 A1 WO 2020116909A1 KR 2019016954 W KR2019016954 W KR 2019016954W WO 2020116909 A1 WO2020116909 A1 WO 2020116909A1
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WIPO (PCT)
Prior art keywords
coating
membrane
antigen
upper plate
manufacturing
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PCT/KR2019/016954
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English (en)
Korean (ko)
Inventor
김성욱
유지인
정철진
오재훈
Original Assignee
주식회사 엘지화학
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Application filed by 주식회사 엘지화학 filed Critical 주식회사 엘지화학
Priority to CN201980080672.9A priority Critical patent/CN113164059A/zh
Publication of WO2020116909A1 publication Critical patent/WO2020116909A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/41—Detecting, measuring or recording for evaluating the immune or lymphatic systems
    • A61B5/411—Detecting or monitoring allergy or intolerance reactions to an allergenic agent or substance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
    • A61B2562/12—Manufacturing methods specially adapted for producing sensors for in-vivo measurements
    • A61B2562/125—Manufacturing methods specially adapted for producing sensors for in-vivo measurements characterised by the manufacture of electrodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
    • A61B2562/16—Details of sensor housings or probes; Details of structural supports for sensors

Definitions

  • the present invention relates to an allergy diagnostic chip and a method for manufacturing the allergy diagnostic chip.
  • Diagnostic chips for analyzing the components in a sample are usually manufactured by coating an antigen or the like on a membrane-shaped fixture. Thereafter, various biological information can be obtained by reacting the antigen coated on the prepared diagnostic chip with a sample to be analyzed.
  • the diagnostic chip can be largely divided into a dot type and a line type.
  • the dot-type diagnostic chip means a diagnostic chip coated with an antigen or the like in a dot form on the fixture
  • the line-type diagnostic chip means a diagnostic chip coated with an antigen or the like in a line shape on the fixture.
  • the dot-type diagnostic chip has a hassle in which an antigen and the like must be coated on a fixed body in a dot form.
  • the line-type diagnostic chip has an advantage in terms of productivity since it can be used in a strip form by coating the antigen on the fixture in a line form and cutting it into a desired shape.
  • the problem to be solved by the present invention is to manufacture a highly integrated allergy diagnosis chip compared to the prior art.
  • the upper plate is formed a space for receiving a solution containing an antigen, a suction unit provided in the lower portion of the upper plate, and provided in the lower portion of the upper plate Preparing a lower plate to be mounted on the suction unit;
  • the solution containing the antigen in the upper plate is moved downward to form a line in which the antigen is adsorbed on the membrane Forming a coating part in a plurality of coating parts;
  • a method for manufacturing an allergy diagnostic chip is provided.
  • the thickness of the plurality of coating parts may be 0.2 mm to 0.5 mm.
  • a gap between the plurality of coating portions may be 0.5 mm to 0.9 mm.
  • the providing of the membrane may further include providing a protection plate between the suction unit and the membrane to prevent damage to the membrane due to a difference between the negative pressure and the normal pressure.
  • the upper plate is formed a space for receiving a solution containing an antigen, a suction unit provided on the lower portion of the upper plate, and provided on the lower portion of the upper plate Preparing a lower plate to be mounted on the suction unit;
  • the solution containing the antigen in the upper plate is moved downward to form a line in which the antigen is adsorbed on the membrane Forming a coating part in a plurality of coating parts;
  • the negative pressure in the step of forming the coating portion is provided with a method for manufacturing an allergy diagnostic chip formed for 7 seconds or more.
  • the membrane in the form of a sheet; And a plurality of coating parts that are regions coated with an antigen on the membrane.
  • the coating portion has a certain thickness (t), the plurality of coating portions are spaced apart from each other at regular intervals (d), the thickness of the coating portion (t) with respect to the interval (d) between the coating portions
  • a ratio (t/d) of 0.40 to 0.55 is provided for an allergy diagnostic chip.
  • the thickness t of the coating portion may be 0.2 mm to 0.5 mm.
  • the distance d between the plurality of coating parts may be 0.5 mm to 0.9 mm.
  • FIG. 1 is a perspective view showing the structure of a manufacturing apparatus that can be used in a method for manufacturing an allergy diagnostic chip according to the present invention.
  • FIG. 2 is a cross-sectional side view showing the structure of a manufacturing apparatus that can be used in a method for manufacturing an allergy diagnostic chip according to the present invention.
  • FIG. 3 is a plan view showing the structure of the suction unit of the manufacturing apparatus that can be used in the present invention.
  • FIG. 4 is a side cross-sectional view showing the structure of the suction unit of the manufacturing apparatus that can be used in the present invention.
  • FIG. 5 is a perspective view showing the structure of an example of the top plate of the manufacturing apparatus that can be used in the present invention.
  • FIG. 6 is a plan view showing the structure of an example of the top plate of the manufacturing apparatus that can be used in the present invention.
  • FIG. 7 is a cross-sectional view showing the structure of the upper plate cut along the line A-A in FIG. 6.
  • FIG. 8 is a perspective view showing the structure of another example of the top plate of the manufacturing apparatus that can be used in the present invention.
  • FIG. 9 is a plan view showing the structure of another example of the top plate of the manufacturing apparatus that can be used in the present invention.
  • FIG. 10 is a cross-sectional view showing the structure of the upper plate cut along the line B-B in FIG. 9.
  • FIG. 11 is a perspective view showing the structure of the lower plate of the manufacturing apparatus that can be used in the present invention.
  • FIG. 12 is a side cross-sectional view showing the structure of the lower plate cut along the line C-C of FIG. 11.
  • FIG. 13 is a plan view showing the structure of an allergy diagnostic chip manufactured according to the present invention.
  • FIG. 14 is a view showing a coating state of a diagnostic chip manufactured in accordance with Examples and Comparative Examples of the present invention.
  • FIG. 1 is a perspective view showing the structure of a manufacturing apparatus that can be used in a method for manufacturing an allergy diagnostic chip according to the present invention
  • FIG. 2 is a side view showing a structure of a manufacturing apparatus that can be used in a method for manufacturing an allergy diagnostic chip according to the present invention It is a cross section.
  • the allergy diagnostic chip manufacturing apparatus 10 (hereinafter, referred to as a'manufacturing device') according to the present invention includes an upper plate 100 in which a solution containing an antigen can be accommodated. It can contain.
  • the upper plate 100 may be configured to receive a solution containing an antigen from the outside and discharge it downward.
  • a suction unit 200 that can move the solution containing the antigen contained in the upper plate 100 downwards due to a pressure difference may be provided at a lower portion of the upper plate 100.
  • the suction unit 200 according to the present invention moves the outside air downward of the suction unit 200 by the difference between the pressure inside the suction unit and the external pressure, as will be described below, so that the inside of the upper plate 100 It may be configured to discharge the solution containing the antigen of the downward.
  • a lower plate 150 mounted on the suction unit 200 may be provided below the upper plate 100.
  • the method for manufacturing an allergy diagnostic chip according to the present invention includes an upper plate 100 and an upper plate 100 in which a space in which a solution containing an antigen is accommodated is formed. It may include the step of preparing a suction plate 200 provided on the lower portion, and a lower plate 150 provided on the lower portion of the upper plate 100 and mounted on the suction unit 200. In addition, the method for manufacturing an allergy diagnostic chip according to the present invention may further include a membrane providing step of providing a membrane on the upper surface of the lower plate 150.
  • FIG. 3 is a plan view showing the structure of the suction unit among the manufacturing devices that can be used in the present invention
  • FIG. 4 is a side cross-sectional view showing the structure of the suction unit among the manufacturing devices that can be used in the present invention.
  • the suction unit 200 may be formed with a space portion 210 which is a space where the upper portion is open, and the lower plate 150 may be mounted on the upper portion of the space portion 210. Can be.
  • the solution containing the antigen accommodated inside the top plate 100 may be discharged downward by the suction unit.
  • an upper surface of the lower plate 150 is provided with a membrane having a thin thickness, and the solution discharged downward is sprayed onto the membrane to coat the antigen on the membrane. .
  • the suction unit 200 may be provided with a flow path 220 that provides a path through which air is transferred.
  • a plurality of flow paths 220 may be provided.
  • FIG. 4 shows a case where three flow paths 220 are provided.
  • the suction unit 200 is connected to the flow path 220 and may be formed with a sound pressure forming unit (not shown) capable of forming sound pressure therein.
  • a sound pressure forming unit capable of forming sound pressure therein.
  • the suction unit 200 when the air existing inside the negative pressure forming portion is removed, the inside of the negative pressure forming portion is in a negative pressure state that is lower than the normal pressure. Therefore, there is a pressure difference between the external pressure (ie, normal pressure) and the internal pressure (ie, negative pressure) of the town pressure forming unit.
  • the flow path 220 of the suction unit 200 is opened, the outside air is supplied to the inside of the negative pressure forming portion by the difference between the pressure inside the negative pressure forming portion and the external pressure, and at the same time inside the upper plate 100.
  • the received antigen is sprayed onto the membrane and coated.
  • a solution containing an antigen in the upper plate 100 is formed by forming a negative pressure, which is a pressure lower than normal pressure, inside the suction unit 200. It may include a step of forming a coating portion by moving downward to form a plurality of coating portions in the form of a line adsorbed with an antigen on the membrane.
  • the negative pressure in the negative pressure forming unit may be formed by discharging the air in the negative pressure forming unit to the outside. Thereafter, air is introduced into the negative pressure forming unit in the forming of the coating unit, and accordingly, the pressure in the negative pressure forming unit gradually increases. Then, when the pressure in the negative pressure forming portion becomes equal to the normal pressure, the coating portion forming step may be ended.
  • a negative pressure may be formed for 7 seconds to 120 seconds.
  • the negative pressure is formed for less than 7 seconds in the step of forming the coating part, in the process of coating the antigen on the membrane, the solution containing the antigen may not be properly coated in the form of a line on the membrane and smear may occur.
  • the negative pressure is formed in the coating part forming step in excess of 120 seconds, the process of coating the antigen on the membrane consumes too much time and productivity may decrease. More preferably, in the step of forming the coating portion, the negative pressure may be formed for 7 seconds to 90 seconds.
  • the difference between the negative pressure and the normal pressure may be 1.8 bar to 2.2 bar.
  • the difference between the negative pressure and the normal pressure in the forming step of the coating is less than 1.8 bar, the speed of spraying the solution in the upper plate 100 during the antigen coating process of the membrane becomes insufficient, and thus the quality of the antigen coating may be deteriorated.
  • the difference between the negative pressure and the normal pressure in the forming step of the coating exceeds 2.2 bar, the speed at which the solution is sprayed during the antigen coating process of the membrane and the speed of the air entering the eup pressure forming portion become too large, and the membrane may be damaged. Can be.
  • the manufacturing apparatus 10 may further include a pressing unit 300 that presses the upper plate 100 downward.
  • the pressing unit 300 according to the present invention lowers the upper plate 100 in the process of coating the antigen on the membrane and adheres to and presses the lower plate 150 so that the antigen is applied to the membrane provided on the upper surface of the lower plate 150. It may be a configuration that allows the coating to be smooth.
  • the pressing unit 300 includes a side pressing portion 310 pressing both sides of the upper portion of the upper plate 100 downward and a central pressing portion pressing the central portion of the upper plate 100 downward. It may include 320.
  • the side pressing portion 310 and the central pressing portion 320 may be driven independently of each other.
  • the method for manufacturing an allergy diagnostic chip according to the present invention may further include a pressing step of further pressing a part of the upper region of the coating unit so that the membrane can be closely adhered downward.
  • the pressing step according to the present invention may be performed between the above-described membrane provision step and the coating part forming step.
  • a step-shaped step 310a may be formed inside the side pressing part 310.
  • the step 310a may have a shape corresponding to both sides of the top plate 100.
  • both sides of the upper plate 100 are vertically formed, and a step 310a facing both sides of the upper plate 100 is also vertically formed.
  • the side pressing portion 310 not only serves to press the top plate 100 downward, but also the top plate 100 It may also play a role of gripping (grip) the top plate 100 so as not to shake from side to side.
  • FIG. 5 is a perspective view showing an example structure of an upper plate among manufacturing apparatuses that can be used in the present invention
  • FIG. 6 is a plan view showing an example structure of an upper plate among manufacturing apparatuses that can be used in the present invention
  • FIG. 7 Is a cross-sectional view showing the structure of the upper plate cut along the line AA in FIG. 6.
  • the upper plate 100 is provided at the upper portion 112 and the lower portion of the upper portion 112 where an injection hole 112a in which a solution containing an antigen is injected is formed. It may include a lower portion 114, the antigen-containing solution is discharged toward the lower plate (150, see FIG. 2).
  • the injection hole 112a of the top plate 100 may be a plurality of supply holes having a dot shape.
  • reference numeral 112a will be referred to as a'supply hole' in this specification.
  • a plurality of slits 114a having a line shape may be formed on the lower surface portion 114.
  • the solution containing the antigen is discharged in the form of a line through a plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 so that the antigen can be coated in a line form on the membrane.
  • the slit 114a does not mean a physical configuration, but a space in which a solution containing an antigen is discharged.
  • the width of the plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 may be 0.2 mm to 0.5 mm.
  • the width of the plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 is 0.2 mm to 0.5 mm, so that when the solution containing the antigen is coated in a line, the membrane is coated on the membrane.
  • the line thickness may also be set to be 0.2 mm to 0.5 mm. Therefore, by reducing the thickness of the line coated on the membrane compared to the prior art, it is possible to achieve high integration of the diagnostic chip. More preferably, the width of the plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 may be 0.3 mm to 0.5 mm.
  • the width of the thickness slit 114a of the coating portion can correspond to That is, the thickness of the plurality of coating portions formed in the coating portion forming step may be 0.2 mm to 0.5 mm. More preferably, the thickness of the plurality of coating parts may be 0.3 mm to 0.5 mm.
  • the upper plate 100 is formed on the upper surface portion 112 and the lower surface portion 114 and connects the connection portion 116 connecting the upper surface portion 112 and the lower surface portion 114. It can contain. It may be understood that the connecting portion 116 of the top plate 100 forms the body of the top plate 100.
  • a plurality of thin plates 116a may be spaced apart from each other inside the connection portion of the upper plate 100.
  • a plurality of inner spaces closed to each other may be formed between the plurality of thin plates 116a, and the solution containing the antigen may be accommodated in the inner space between the plurality of thin plates 116a.
  • the plurality of inner spaces formed by the plurality of thin plates 116a provided on the upper plate 100 may communicate with one of the plurality of slits 114a formed on the lower surface of the upper plate 100, respectively.
  • each of the plurality of supply holes 112a formed in the upper surface portion 112 of the upper plate 100 may communicate with one of the plurality of internal spaces formed in the connection portion 116 of the upper plate 100.
  • the solution containing the antigen supplied through one of the supply holes 112a of the upper plate 100 is formed by a plurality of thin plates 116a and communicates with the supply hole 112a in a plurality of internal spaces. After flowing in, it may be discharged downward through the slits 114a communicating with the plurality of internal spaces.
  • the thickness of the plurality of thin plates 116a formed on the connecting portion 116 of the upper plate 100 may be 0.5 mm to 0.9 mm.
  • slits 114a formed in the lower surface portion 114 of the upper plate 100 are formed of a plurality of inner spaces formed by a plurality of thin plates 116a provided in the connection portion 116. It can be connected to the end.
  • the spacing between the slits 114a formed in the lower surface portion 114 of the upper plate 100 may be the same as the thickness of the plurality of thin plates 116a provided in the connection portion 116. Therefore, the thickness of the thin plate 116a may be the same as the spacing between the coating parts formed in the coating part forming step.
  • the interval between the plurality of coating parts in the step of forming the coating part of the method for manufacturing an allergy diagnostic chip according to the present invention may be 0.5 mm to 0.9 mm.
  • the gap between the plurality of coating parts is less than 0.5 mm, the thin plate 116a may be damaged by a rapid flow of fluid by the operation of the suction unit 200 in the process of coating the antigen on the membrane.
  • the spacing between the plurality of coating parts exceeds 0.9 mm, the width between the coating parts coated on the membrane becomes too large, making it impossible to highly integrate the diagnostic chip.
  • the supply hole 112a of the upper surface portion formed in the upper plate 100, the inner space formed by the plurality of thin plates 116a, and the slits 114a of the lower surface portion may be in one-to-one communication with each other. Accordingly, the number of the plurality of supply holes 112a formed in the upper surface portion 112 of the upper plate 100, the number of the plurality of slits 114a formed in the lower surface portion 114 of the upper plate 100, and the upper plate The number of the plurality of internal spaces formed in the connection portion 116 of the (100) may be the same as each other. In FIG.
  • FIG. 7 some of the plurality of internal spaces formed in the connection portion 116 of the upper plate 100 are illustrated as not being in communication with the supply hole 112a of the upper surface portion 112, but as illustrated in FIG. 6 Since the supply hole 112a may be formed over the entire area of the upper surface portion 112 of the upper plate, it should not be understood that the contents shown in FIG. 7 do not correspond to the above contents of this paragraph.
  • the supply hole 112a of the upper surface portion formed in the upper plate 100 may correspond to an inner space formed by a plurality of thin plates 116a and n (n is an integer of 2 or more) to 1.
  • the supply hole 112a of the upper surface portion formed in the upper plate 100 may correspond to the inner space formed by the plurality of thin plates 116a in a two-to-one manner.
  • Figure 8 is a perspective view showing the structure of another example of the top plate of the manufacturing apparatus that can be used in the present invention
  • Figure 9 is a plan view showing the structure of another example of the top plate in the manufacturing apparatus that can be used in the present invention
  • Figure 10 Is a cross-sectional view showing the structure of the upper plate cut along the line BB in FIG. 9.
  • a part overlapping with the description of an example of the top plate among the description of another example of the top plate will be omitted, and the description will be mainly focused on contents different from the example of the top plate.
  • the upper surface portion 112 of the upper plate 100 has a slit 112b having a line shape so that a solution containing an antigen can be supplied to the upper plate 112
  • the injection hole may be formed in plural, and each of the plurality of slits 112b formed in the upper surface portion 112 of the upper plate 100 may be among the plurality of inner spaces formed in the connection portion 116 of the upper plate 100. Can communicate with one.
  • the solution containing the antigen supplied through one of the slits 112b of the upper surface portion 112 of the top plate 100 is formed by a plurality of thin plates 116a and communicated with the slits 112b. After entering the plurality of interior spaces, the slits 114a of the lower surface portion 114 communicating with the plurality of interior spaces may be discharged downward.
  • the slit 112b of the upper surface portion formed in the upper plate 100, the inner space formed by the plurality of thin plates 116a, and the slit 114a of the lower surface portion may be in one-to-one communication with each other. Accordingly, the number of the plurality of slits 112b formed in the upper surface portion 112 of the upper plate 100, the number of the plurality of slits 114a formed in the lower surface portion 114 of the upper plate 100, and the upper plate ( The number of internal spaces formed in the connecting portion 116 of 100) may be the same. In FIG.
  • connection portion 116 of the upper plate 100 some of the plurality of internal spaces formed in the connection portion 116 of the upper plate 100 are shown as not communicating with the slit 112b of the upper surface portion 112, but as shown in FIG. 9 Since 112b is formed over the entire area of the upper surface portion 112 of the top plate, it should not be understood that the contents shown in FIG. 9 do not match the contents of the paragraph above.
  • the upper plate 100 may have a shape formed by mixing the aforementioned supply hole 112a and the slit 112b. That is, the upper plate 100 according to the present invention may have a structure in which both the supply hole 112a and the slit 112b are formed.
  • FIG. 11 is a perspective view showing the structure of the lower plate of the manufacturing apparatus that can be used in the present invention
  • FIG. 12 is a side cross-sectional view showing the structure of the lower plate cut along the line C-C of FIG. 11.
  • the lower plate 150 may include an upper surface portion 162 on which a plurality of slits 162a are formed and a lower surface portion 164 on which a plurality of slits 164a are formed.
  • the slits 162a and 164a formed in the lower plate 150 also mean a physical space.
  • the slits 162a and 164a respectively formed on the upper surface portion 162 and the lower surface portion 164 of the lower plate 150 are coated with an antigen on the membrane, and external air is introduced into the suction unit. You can provide a route. That is, when the negative pressure is formed inside the suction unit 200 during the coating process of the membrane as described above, the outside air is formed with a slit 162a and a lower surface 164 formed in the upper surface portion 162 of the lower plate 150. After passing through the slit (164a) formed in sequence may be supplied to the suction unit.
  • the lower plate 150 may be formed with a connecting portion connecting the upper surface portion 162 and the lower surface portion 164, and a plurality of thin plates spaced apart from each other may be formed inside the connecting portion. have. Each of the inner spaces between the plurality of thin plates may communicate with one of the slits 162a of the upper surface portion 162 and one of the slits 164a of the lower surface portion 164.
  • the ratio of the width of the slit to the thickness of the thin plate in the upper plate according to the present invention may be 0.40 to 0.55.
  • FIG. 13 is a plan view showing the structure of an allergy diagnostic chip manufactured according to the present invention.
  • the allergy diagnostic chip 400 according to the present invention may be configured by the method for manufacturing an allergy diagnostic chip according to the present invention.
  • the allergy diagnostic chip 400 may include a sheet-shaped membrane 400a and a coating unit 410 that is an antigen-coated region on the membrane 400a.
  • a plurality of coating parts 410 may be formed, and may have a band shape having a constant thickness.
  • the allergy diagnostic chip 400 may have a rectangular shape having a constant width (W) and a length (L), and the plurality of coating parts 410 having a constant thickness (t) may have a width ( W) may be provided spaced apart from each other by a constant distance (d) along the direction.
  • the allergy diagnostic chip 400 according to the present invention can form a larger number of coating parts compared to the same area of the chip as compared to the prior art, so that the allergy diagnostic chip 400 can be highly integrated. To this end, in the allergy diagnostic chip 400 according to the present invention, it may have a constant ratio between the distance d between the coating parts 410 and the thickness t of the coating parts 410.
  • the ratio (ie, t/d) of the thickness (t) of the coating portion 410 to the distance (d) between the coating portions 410 in the allergy diagnostic chip 400 according to the present invention may be 0.40 to 0.55 have.
  • the thickness t of the coating unit 410 may be 0.2 mm to 0.5 mm.
  • the distance d between the coating parts 410 may be 0.5 mm to 0.9 mm.
  • the present invention controls the difference between the normal pressure and the negative pressure within a certain numerical range in the step of forming the coating, damage to the membrane may occur.
  • the step of forming the coating part external air is introduced into the suction device through the lower plate by a pressure difference between negative pressure and normal pressure, and the membrane provided on the upper surface of the lower plate 150 has an upper surface of the lower plate 150.
  • problems such as being sucked into the slit 162a of the upper surface portion 162 may occur.
  • the membrane provision step of the method for manufacturing an allergy diagnostic chip according to the present invention includes a plate for damaging membrane damage due to a difference between negative pressure and normal pressure between the suction unit 200 and the membrane. It may further include having.
  • the protective plate may include a cotton material. When the protective plate includes a cotton material or is made of a cotton material, it is possible to prevent the membrane from being damaged due to the material of the protective plate itself in the step of forming the coating.
  • a membrane was provided on the upper surface of the lower plate.
  • the side pressing portion and the central pressing portion were moved downward to adhere the upper plate to the lower plate and the membrane.
  • the air inside the negative pressure forming portion of the suction unit was discharged to create a negative pressure state that is 2 bar lower than normal pressure.
  • the outside air flows into the negative pressure forming portion, and a solution containing the antigen is coated on the membrane to prepare a diagnostic chip.
  • the flow path of the suction unit was opened for 7 seconds.
  • the pressure in the negative pressure forming portion gradually increased, and after the coating was completed, the pressure in the negative pressure forming portion reached normal pressure.
  • the membrane was coated with a solution containing the antigen in the same manner as in Example 1, except that the flow path of the suction unit was opened for 30 seconds.
  • the membrane was coated with a solution containing the antigen in the same manner as in Example 1, except that the flow path of the suction unit was opened for 60 seconds.
  • the membrane was coated with a solution containing the antigen in the same manner as in Example 1, except that the flow path of the suction unit was opened for 120 seconds.
  • the membrane was coated with a solution containing an antigen in the same manner as in Example 1, except that the flow path of the suction unit was opened for 5 seconds.
  • FIG. 14(a) shows the coating state of the diagnostic chip according to the comparative example
  • FIGS. 14(b) to 14(e) show the coating state of the diagnostic chip according to Examples 1 to 4, respectively. will be.
  • Fig. 14 (a) in the case of the comparative example, it was confirmed that a part of the solution containing the antigen was smeared around the line coated on the diagnostic chip. That is, in the case of the comparative example, it was confirmed that the time for forming the negative pressure was not sufficiently secured, so that the coating on the membrane was not properly performed.
  • the thickness (t) of the coating layer coated with the antigen-containing solution and the gap (d) between the coating layers were measured.
  • the thickness (t) of the coating layer was measured to be 0.4 mm, respectively, and the spacing (d) between the coating layers was measured to be 0.75 mm. Therefore, the ratio (t/d) of the thickness t of the coating portion to the gap d between the coating portions was measured to be 0.533.

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Abstract

L'invention concerne une puce de diagnostic d'allergie et un procédé de fabrication de la puce de diagnostic d'allergie. La présente invention concerne un procédé de fabrication d'une puce de diagnostic d'allergie, le procédé comprenant : une étape de formation de partie de revêtement consistant à former une pression négative inférieure à une pression normale dans une unité d'aspiration en vue de déplacer vers le bas une solution contenant un antigène dans une plaque supérieure, de manière à former une pluralité de parties de revêtement de type ligne dans lesquelles l'antigène est adsorbé sur une membrane.
PCT/KR2019/016954 2018-12-07 2019-12-03 Puce de diagnostic d'allergie et procédé de fabrication d'une puce de diagnostic d'allergie WO2020116909A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201980080672.9A CN113164059A (zh) 2018-12-07 2019-12-03 过敏诊断芯片及过敏诊断芯片的制造方法

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Application Number Priority Date Filing Date Title
KR1020180157440A KR102051434B1 (ko) 2018-12-07 2018-12-07 알러지 진단용 칩 및 그 알러지 진단용 칩 제조 방법
KR10-2018-0157440 2018-12-07

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WO2020116909A1 true WO2020116909A1 (fr) 2020-06-11

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