WO2020103616A1

WO2020103616A1 - Copper oxide/carbon nanotube/carbon nitride electrochemical sensor for detecting acetaminophen, preparation method therefor and application thereof

Info

Publication number: WO2020103616A1
Application number: PCT/CN2019/111754
Authority: WO
Inventors: 张菲菲; 袁月环; 王宗花; 王海燕
Original assignee: 青岛大学
Priority date: 2018-11-22
Filing date: 2019-10-17
Publication date: 2020-05-28
Also published as: CN109298049A; CN109298049B

Abstract

A copper oxide/carbon nanotube/carbon nitride electrochemical sensor for detecting acetaminophen, a preparation method therefor and an application thereof. The method comprises: mixing carboxylated carbon nanotubes, carbon nitride, and water uniformly, and performing sonication; then adding copper acetate, mixing uniformly, and performing sonication; and drying after the sonication, and then calcining in an inert gas atmosphere to obtain a copper oxide/carbon nanotube/carbon nitride composite material. A CuO/CNT/g-C3N4 composite modified electrode is prepared and used as an electrochemical sensor for detecting acetaminophen. The electrochemical result shows that the composite material has a good detection effect on acetaminophen.

Description

Copper oxide / carbon nanotube / carbon nitride electrochemical sensor for detecting acetaminophen and preparation method and application thereof

Technical field

The present disclosure belongs to the field of electrochemical analysis and detection, and in particular relates to a copper oxide / carbon nanotube / carbon nitride electrochemical sensor for detecting acetaminophen and its preparation method and application.

Background technique

The statements herein provide only background information related to the present disclosure and do not necessarily constitute prior art.

Acetaminophen (N-acetyl-P-aminophenol or acetaminophen) is one of the most commonly used analgesics and is widely used to relieve fever, cold, cough and pain, such as headache, toothache and back pain, etc. Illness. Acetaminophen does not have any serious side effects when taken at the prescribed dose. However, excessive use of acetaminophen can lead to the accumulation of toxic metabolites, which can lead to liver toxicity and nephrotoxicity, which in turn can damage the liver and other organs of the kidney. Therefore, it is necessary to quickly and sensitively detect acetaminophen in clinical applications. There are many methods for detecting acetaminophen, such as: titration analysis, spectrophotometry, electrochemical method, chemiluminescence method, high performance liquid chromatography and high performance capillary electrophoresis. Compared with other technologies, electrochemical sensing provides an application platform for its fast, accurate and convenient detection process.

Summary of the invention

In order to enrich the types of acetaminophen electrochemical sensors and obtain a good detection effect for acetaminophen, in the first aspect of the present disclosure, a copper oxide / carbon nanotube / carbon nitride composite material is provided The preparation method includes: mixing carboxylated carbon nanotubes, carbon nitride and water uniformly, and performing ultrasonic treatment; then adding copper acetate, mixing and performing ultrasonic treatment; drying after ultrasonic treatment, and then inert gas atmosphere The calcination is carried out under the condition of copper oxide / carbon nanotube / carbon nitride composite material.

In a second aspect of the present disclosure, a copper oxide / carbon nanotube / carbon nitride composite material prepared by the above method is provided.

In a third aspect of the present disclosure, the application of the copper oxide / carbon nanotube / carbon nitride composite material in preparing an electrochemical sensor for detecting acetaminophen is provided.

In a fourth aspect of the present disclosure, there is provided an electrochemical sensor for detecting acetaminophen, the electrochemical sensor including a substrate electrode; and,

The above copper oxide / carbon nanotube / carbon nitride composite material is attached to the base electrode.

In a fifth aspect of the present disclosure, a method for preparing the electrochemical sensor for detecting acetaminophen is provided. The method includes the following steps:

First, the obtained copper oxide / carbon nanotube / carbon nitride composite material is washed with water, dried, and dispersed into a dispersion liquid with a concentration of 0.5 to 1.5 mg / ml in water: secondly, the base electrode is polished with aluminum powder Separately use ethanol and secondary water for ultrasonic cleaning. After blowing dry with nitrogen, apply the dispersed droplets to the electrode to prepare a copper oxide / carbon nanotube / carbon nitride modified electrode, which is used to detect acetaminophen. Chemical sensor.

In a sixth aspect of the present disclosure, the application of the electrochemical sensor in detecting acetaminophen is provided.

In a seventh aspect of the present disclosure, a method for detecting paracetamol is provided, the method including the step of performing electrochemical detection using the detection paracetamol electrochemical sensor.

Compared with related technologies known to the inventor, one of the technical solutions of the present disclosure has the following beneficial effects:

In the present disclosure, gC ₃ N ₄ is compounded with carboxylated carbon nanotubes (CNT), and then copper acetate solution is added, and calcined at high temperature to obtain copper oxide / carbon nanotube / carbon nitride (CuO / CNT / gC ₃ N ₄ ) Composite materials. Wherein the strong complexation between the gC ₃ N ₄ N atoms and the Cu atom can even load CuO gC ₄ to the surface ₃ N. At the same time, the lone pair of nitrogen atoms in carbon nitride has a conjugated effect on the π bond between the carbon nanotubes, allowing the carbon nanotubes to enter the carbon nitride layer, increasing the surface area of the composite material, and at the same time the overall composite material The electrical conductivity of the material has been improved, which in turn enables the composite material to have enhanced catalytic capabilities. A CuO / CNT / gC ₃ N ₄ composite modified electrode was prepared and used as an electrochemical sensor to detect acetaminophen. The electrochemical results show that the composite material has a good detection effect on acetaminophen.

BRIEF DESCRIPTION

The accompanying drawings forming part of this disclosure are used to provide a further understanding of this disclosure. The exemplary embodiments of this disclosure and their descriptions are used to explain this disclosure and do not constitute an undue limitation of this disclosure.

Figure 1 shows the bare electrode (a), gC ₃ N ₄ (b), CuO (c), CNT (d), CNT / gC ₃ N ₄ (e), CuO / CNT / gC ₃ N ₄ (f) modified electrode The CV curve in a PBS buffer solution (pH = 7.00) with a paracetamol concentration of 200 μM has a sweep rate of 100 mV / s.

Figure 2 SWV curves of CuO / CNT / gC ₃ N ₄ modified electrode measured in different concentrations of acetaminophen in PBS buffer solution (pH = 7.0). Among them, the acetaminophen concentration is 0.02 μM, 0.04 μM, 0.06 μM, 0.08 μM, 0.1 μM (A, B), 1 μM, 15 μM, 30 μM, 45 μM, 60 μM, 75 μM, 90 μM, 105 μM, 120 μM (C, D).

3 is a transmission electron micrograph of carbon nitride (A), carbon nanotube / carbon nitride (B), and copper oxide / carbon nanotube / carbon nitride (C).

detailed description

It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanations of the present disclosure. Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this disclosure belongs.

It should be noted that the terminology used herein is for describing specific embodiments only, and is not intended to limit exemplary embodiments according to the present disclosure. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should also be understood that when the terms "comprising" and / or "including" are used in this specification, There are features, steps, operations, and / or combinations thereof.

In a first typical embodiment of the present disclosure, a method for preparing a copper oxide / carbon nanotube / carbon nitride composite material is provided. The method includes the following steps: the carboxylated carbon nanotube, carbon nitride, and water Mix evenly and perform ultrasonic treatment; then add copper acetate, mix and perform ultrasonic treatment; after ultrasonic treatment, dry and then calcinate under an inert gas atmosphere to obtain copper oxide / carbon nanotube / carbon nitride composite materials after calcination.

The internal structure of gC ₃ N ₄ has a rich and uniform nitrogen coordination agent, which can provide more metal coordination sites as catalytic active sites during the electrocatalysis process. The strong coordination between the N atom and Cu atom in gC ₃ N ₄ can increase the active site of the composite material, thereby improving the overall electrocatalytic activity of the material. CuO / CNT / gC ₃ N ₄ composite materials prepared with different proportions of raw materials have different catalytic properties. In one or some specific embodiments of the present disclosure, the carboxylated carbon nanotubes, carbon nitride, and water The ratio to copper acetate is (10-30) mg: (5-15) mg: (5-20) mL (5-6) mg. It has been verified by experiments that the above-mentioned ratio makes the CuO / CNT / gC ₃ N ₄ composite material have better catalytic performance and a good detection effect of acetaminophen.

In one or some specific embodiments of the present disclosure, the carboxylated carbon nanotubes, carbon nitride, and water are uniformly mixed, and the ultrasonic treatment time is 1 to 2.5 hours. Further, the sonication time is 2h.

When preparing a CuO / CNT / gC ₃ N ₄ composite material, the present disclosure first composites gC ₃ N ₄ with carboxylated carbon nanotubes so that the carbon nanotubes can enter the gC ₃ N ₄ layer, increasing the composite material At the same time, this operation makes the overall conductivity of the composite material well improved.

In one or some specific embodiments of the present disclosure, copper acetate is added and mixed, and the ultrasonic treatment time is 3 to 4 hours. Further, the sonication time is 3h.

If the calcination temperature is too high or too low, the copper oxide / carbon nanotube / carbon nitride composite material with ideal morphology cannot be obtained. In one or some specific embodiments of the present disclosure, the calcination temperature is 500-600 ° C, and the calcination time is 2-4h. Further, the calcination temperature is 520 ° C and the calcination time is 3h.

In one or some specific embodiments of the present disclosure, the inert gas is argon or helium.

In one or some specific embodiments of the present disclosure, a method for preparing carboxylated carbon nanotubes is provided. The method includes: mixing carbon nanotubes with concentrated nitric acid, then performing ultrasonic treatment, and then cleaning with water until neutral, Finally, the washed carbon nanotubes are dried, and then taken out to obtain dried carboxylated carbon nanotubes. The purpose of carboxylation is to attach carboxyl groups on the surface of carbon nanotubes, and then combine with amino groups on the surface of carbon nitride to form a composite material.

Further, the carbon nanotubes have a diameter of 40-60 nm and a length greater than 5 μm, and were purchased from Shenzhen Nanoport Technology Co., Ltd. The selection basis is determined according to the size of the carbon nitride material (the size of the carbon nitride nanosheet is about 1-2 μm). The diameter of the carbon nanotubes should not be too large, and the length should be as long as possible, which is conducive to the subsequent reaction.

Further, the addition ratio of the carbon nanotubes and concentrated nitric acid is (30-50) mg: (15-25) mL; the mass fraction of the concentrated nitric acid is 95-98%.

In one or some specific embodiments of the present disclosure, a method for preparing carbon nitride is provided. The method includes:

Using dicyandiamine, urea or melamine as raw materials, primary calcination under an inert gas atmosphere yields a pale yellow solid gC ₃ N ₄ , which is then powdered, followed by secondary calcination under an inert gas atmosphere. Yellow powder gC ₃ N ₄ .

Further, in order to make the yield of gC ₃ N ₄ higher, the raw material is melamine.

Further, the inert gas is argon.

In order to obtain gC ₃ N ₄ with a large surface area, in the primary calcination or secondary calcination, the calcination temperature is 500-600 ° C., and the heating rate is 2.5-3.5 ° C./min.

Further, the calcination temperature is 550 ° C, and the heating rate is 3 ° C / min.

Further, in the primary calcination, the calcination time is 4.5 to 5.5 hours.

Furthermore, the calcination time is 5h.

Further, in the secondary calcination, the calcination time is 1.5 to 2.5 hours. The purpose of secondary calcination under the protection of inert gas is to peel off the carbon nitride nanosheets.

Furthermore, the calcination time is 2h.

The lamellar micromorphology of gC ₃ N ₄ prepared by the above method provides the premise for uniform loading of copper oxide and the foundation for the insertion of carbon nanotubes.

In a second typical embodiment of the present disclosure, a copper oxide / carbon nanotube / carbon nitride composite material prepared by the above method is provided.

Carbon nitride (gC ₃ N ₄ ) is a two-dimensional structure composed of nitrogen substituted carbon six ring units. The inherently rich nitrogen active sites and excellent catalytic activity of gC ₃ N ₄ make it have broad application prospects in many fields. As a p-type semiconductor metal oxide, copper oxide has potential application prospects in the fields of gas sensing, lithium ion batteries, and catalysis. the strong complexation between the gC ₃ N ₄ N atoms and can be made of Cu atoms to CuO uniform loading surface ₃ N ₄ gC, and CuO while gC ₃ N ₄ heterojunction interface between reflector shape, which is conducive to enhance composite The catalytic performance of the material. However, due to poor conductivity, the potential electrochemical catalytic performance of CuO / gC ₃ N ₄ is greatly reduced, which also limits its application in electrochemical sensors. Carbon nanotubes have a unique π-conjugated structure, high conductivity and large specific surface area. Its unique structure can be combined with other nanomaterials such as graphene, ionic liquids, nanoparticles, etc. Chemical sensitivity.

In a third typical embodiment of the present disclosure, the application of the copper oxide / carbon nanotube / carbon nitride composite material in preparing an electrochemical sensor for detecting acetaminophen is provided.

In a fourth exemplary embodiment of the present disclosure, an electrochemical sensor for detecting paracetamol is provided, the electrochemical sensor includes a substrate electrode; and,

The copper oxide / carbon nanotube / carbon nitride composite material is attached to the base electrode.

In one or some specific embodiments of the present disclosure, the base electrode is a glassy carbon electrode.

In a fifth typical embodiment of the present disclosure, a preparation method of the electrochemical sensor for detecting acetaminophen is provided. The method includes:

First, the obtained copper oxide / carbon nanotube / carbon nitride composite material is washed with water, dried, and dispersed into a dispersion liquid with a concentration of 0.5 to 1.5 mg / ml with water: secondly, the base electrode is first polished with aluminum powder, Separately use ethanol and water for ultrasonic cleaning. After blowing dry with nitrogen, apply the dispersed droplets to the electrode to prepare a copper oxide / carbon nanotube / carbon nitride modified electrode, which is an electrochemical sensor for detecting acetaminophen .

In a sixth exemplary embodiment of the present disclosure, the application of the electrochemical sensor in detecting acetaminophen is provided.

In a seventh exemplary embodiment of the present disclosure, a method for detecting acetaminophen for non-diagnostic purposes is provided. The method includes the following steps:

(1) Preparation of standard solution: prepare a set of acetaminophen standard solutions with different concentrations;

(2) Drawing of the working curve: place the electrochemical sensor in the acetaminophen standard solution of different concentrations and perform square wave voltammetry to obtain the response peak current of the acetaminophen standard solution of different concentrations, and then According to the concentration of acetaminophen standard solution and the response peak current, draw a linear relationship curve;

(3) Detection of the sample: place the electrochemical sensor in the sample to be tested for square wave voltammetry to obtain the response peak current of the sample to be tested, and then obtain the response of the sample to be tested according to the linear relationship curve The concentration of acetaminophen.

Further, the linear relationship curve is I (μA) = 0.5024c _APAP (μM) +10.1169, the correlation coefficient is 0.9985, C _CAP is the concentration of acetaminophen, and the concentration range is 1 to 120 μM.

In order to enable those skilled in the art to understand the technical solutions of the present disclosure more clearly, the technical solutions of the present disclosure will be described in detail below in conjunction with specific embodiments.

Example 1 Preparation of carbon nitride (gC ₃ N ₄ )

Weigh 3g of melamine and heat to 550 ° C at a temperature increase rate of 3 ° C / min in an argon atmosphere and hold it for 5 hours. After the end, it was naturally cooled to room temperature and taken out to obtain a light yellow solid carbon nitride solid. The carbon nitride solid obtained above was put into a mortar and ground into a powder, and then calcined again in the same environment under the above synthesis conditions for 2 hours, and after it was naturally cooled to room temperature, a deep yellow carbon nitride powder was obtained. Take 50 mg of carbon nitride and disperse it in secondary water with a dispersion concentration of 2 mg / mL.

Example 2 Carboxylated Carbon Nanotubes (CNT)

Weigh 40mg carbon nanotubes in a beaker, add 20mL concentrated nitric acid (98w / w%), take out after 6 hours of ultrasound, wash with secondary water until neutral, transfer the cleaned carbon nanotubes to a vacuum drying oven, It was dried at 60 ° C for 24 hours, and after taking out, dried carboxylated carbon nanotubes were obtained, which were dispersed in an aqueous solution at a concentration of 2 mg / mL.

Example 3 Preparation of CNT / gC ₃ N ₄ composite material

Take 10 mL of the carbon nanotube aqueous solution in Example 2 (2 mg / mL), add 5 mL of the carbon nitride aqueous solution in Example 1 (2 mg / mL), take out after 5 hours of ultrasound, and vacuum-dry in a vacuum drying test box at 60 ° Transfer to a tube furnace, heat to 520 ° C at a heating rate of 4 ° C / min, and hold for three hours. During this process, argon gas must be passed to protect.

Example 4 Preparation of CuO / CNT / gC ₃ N ₄ composite material

Take 10 mL of the carbon nanotube aqueous solution in Example 2 (2 mg / mL), add 5 mL of the carbon nitride aqueous solution in Example 1 (2 mg / mL), after sonication for 2 hours, add 0.0053 g of copper acetate powder, and continue ultrasonic vibration 3 hour. After the ultrasound is completed, it is vacuum-dried in a vacuum drying test box at 60 ° C and then transferred to a tube furnace, heated to 520 ° C at a heating rate of 4 ° C / min, and maintained for three hours. During this process, it must be protected by argon gas. .

After the second calcination, the carbon nitride nanosheet (in Example 1) exhibits a thin sheet-like structure, and its transmission electron microscope image is shown in FIG. 3A. After the CNT in Example 2 was added, the results shown in FIG. 3B were obtained after sonication for a period of time. It can be seen from the figure that the carbon nanotubes were successfully incorporated into the surface of carbon nitride. Figure 3C shows the transmission electron micrograph of the copper oxide / carbon nanotube / carbon nitride composite material in Example 4. As can be seen from the figure, when copper acetate is added to the ultrasonic stirring and calcination, there are Copper oxide particles appear, whose size is between 20-40nm.

Example 5

Preparation of electrochemical sensor for detecting acetaminophen

First, after washing the obtained copper oxide / carbon nanotube / carbon nitride composite material with secondary water, it is dried in a vacuum drying oven and dispersed into a dispersion liquid with a concentration of 1 mg / ml with secondary water: secondly, the The base electrode was first polished with aluminum powder, ultrasonically cleaned with ethanol and secondary water, and dried with nitrogen, and then 6 microliters of the above dispersed droplets were applied to the electrode to prepare copper oxide / carbon nanotube / carbon nitride The modified electrode is an electrochemical sensor for detecting acetaminophen.

Example 6 Electrochemical test

This test uses cyclic voltammetry (CV) and square wave voltammetry (SWV). The specific operation is to construct the three-electrode system with the prepared modified electrode, the reference electrode (silver / silver chloride electrode) and the counter electrode, and then place the three-electrode system in PBS buffer with a concentration of 0.2mM acetaminophen In the solution (pH = 7.0), the electrochemical detection is finally carried out. In cyclic voltammetry (CV), the sweep speed is set to 100mV / s.

Figure 1 shows the CV curve of different modified electrodes in PBS buffer solution (pH = 7.00) with a paracetamol concentration of 200 μM. It can be seen from the figure that the electric double layer area of the CuO / CNT / gC ₃ N ₄ modified electrode is larger than that of the bare electrode, which shows that the area of the CV curve of the CuO / CNT / gC ₃ N ₄ modified electrode and the area of the CV curve of the bare electrode The comparison is increased. In the presence of acetaminophen, a broad peak appeared in the bare electrode (a) of approximately + 0.45V, which was caused by the electrochemical oxidation of acetaminophen. However, the reduction peak is not significant, indicating that the electron transfer rate occurring at the bare electrode due to the irreversible oxidation process is slow. For the CuO / CNT / gC ₃ N ₄ modified electrode, a pair of reversible redox peaks appeared between + 0.3V and + 0.4V. At the same time, the acetamido group of the CuO / CNT / gC ₃ N ₄ modified electrode Compared with the oxidation peak of the bare electrode, the peak current value of phenol is about 6 times that of the bare electrode. In addition, the redox peak potential of the CuO / CNT / gC ₃ N ₄ modified electrode is shifted relative to the bare electrode, which also shows that the CuO / CNT / gC ₃ N ₄ modified electrode can effectively promote the oxidation of acetaminophen . The position and intensity of the redox peak in the CV curve indicate that the prepared CuO / CNT / gC ₃ N ₄ modified electrode has a good catalytic effect on acetaminophen.

Example 7 A method for detecting acetaminophen

(2) Drawing of working curve: place the electrochemical sensor of Example 5 in the acetaminophen standard solutions of different concentrations and perform square wave voltammetry to obtain the response peak current of acetaminophen standard solutions of different concentrations , And then draw a linear relationship curve according to the concentration of acetaminophen standard solution and the response peak current;

(3) Detection of the sample: place the electrochemical sensor of Example 5 in the sample to be tested for square wave voltammetry to obtain the response peak current of the sample to be tested, and then obtain the sample to be tested according to the linear relationship curve The concentration of acetaminophen in the medium.

Fig. 2 shows that under the optimal conditions (0.1M phosphate buffer solution with pH 7), the electrochemical sensor of Example 5 was used to detect different concentrations of acetaminophen by square wave voltammetry. As shown in the figure, as the concentration of acetaminophen increases, the peak oxidation current gradually increases. It can be seen from Figures 2A and 2B that the peak current of oxidation is linearly related to the concentration of acetaminophen. The linear equation is: I (μA) = 51c _APAP (μM) + 0.394, the correlation coefficient is 0.9959, the concentration range It is 0.02 to 0.1μM; as can be seen from Figure 2C and Figure 2D, the oxidation peak current is linearly related to the acetaminophen concentration, the linear equation is: I (μA) = 0.5024c _APAP (μM) + 10.11169, The correlation coefficient is 0.9985 and the concentration range is 1 to 120 μM; the lowest detection line is 0.00609 μM (S / N = 3).

The above embodiments are preferred embodiments of the present disclosure, but the embodiments of the present disclosure are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, changes, modifications, substitutions, combinations, The simplifications should all be equivalent replacement methods, which are all included in the protection scope of the present disclosure.

Claims

A method for preparing a copper oxide / carbon nanotube / carbon nitride composite material, characterized in that the method includes the following steps: mixing the carboxylated carbon nanotube, carbon nitride and water uniformly, and performing ultrasonic treatment; then adding acetic acid Copper, mix well, and perform ultrasonic treatment; after ultrasonic treatment, dry, and then calcinate in an inert gas atmosphere, and then obtain a copper oxide / carbon nanotube / carbon nitride composite material after calcination.
The preparation method according to claim 1, wherein the ratio of carboxylated carbon nanotubes, carbon nitride, water and copper acetate is (10-30) mg: (5-15) mg: (5-20) mL (5 ～ 6) mg;

Further, the carboxylated carbon nanotubes, carbon nitride and water are mixed evenly, and the ultrasonic treatment time is 1 to 2.5 hours;

Further, add copper acetate, mix well, ultrasonic treatment time is 3 ~ 4h;

Further, the calcination temperature is 500 to 600 ° C, and the calcination time is 2 to 4 hours;

Further, the inert gas is argon or helium.
The preparation method according to claim 1, characterized in that the preparation method of the carboxylated carbon nanotubes includes: mixing the carbon nanotubes and concentrated nitric acid, then performing ultrasonic treatment, and then cleaning with water until neutral, and finally The cleaned carbon nanotubes are dried, and after taking out, dried carboxylated carbon nanotubes are obtained;

Further, the diameter of the carbon nanotube is 40-60 nm, and the length is greater than 5 μm;

Further, the addition ratio of the carbon nanotubes and concentrated nitric acid is (30-50) mg: (15-25) mL; the mass fraction of the concentrated nitric acid is 95-98%; or,

The preparation method of carbon nitride, the method includes the following steps:

Using dicyandiamine, urea or melamine as raw materials, primary calcination under an inert gas atmosphere yields a pale yellow solid gC 3 N 4 , which is then powdered, followed by secondary calcination under an inert gas atmosphere. Yellow powder gC 3 N 4 ;

Further, in the primary calcination or secondary calcination, the calcination temperature is 500-600 ° C, and the heating rate is 2.5-3.5 ° C / min;

Further, in one calcination, the calcination time is 4.5-5.5h;

Further, in the secondary calcination, the calcination time is 1.5 to 2.5 hours.
The copper oxide / carbon nanotube / carbon nitride composite material prepared by the method according to any one of claims 1 to 3.
The application of the copper oxide / carbon nanotube / carbon nitride composite material according to claim 4 in the preparation and detection of an acetaminophen electrochemical sensor.
An electrochemical sensor for detecting paracetamol, characterized in that the electrochemical sensor includes a base electrode; and,

The copper oxide / carbon nanotube / carbon nitride composite material according to claim 4, which is attached to the base electrode;

Further, the base electrode is a glassy carbon electrode.
The preparation method of the electrochemical sensor for detecting acetaminophen according to claim 6, wherein the method comprises:

First, the copper oxide / carbon nanotube / carbon nitride composite material is washed with water, dried, and dispersed into a dispersion liquid with a concentration of 0.5 to 1.5 mg / ml with water: secondly, the base electrode is first polished with aluminum powder, respectively Ethanol and water are ultrasonically cleaned, dried with nitrogen, and the dispersed droplets are coated on the electrode to prepare a copper oxide / carbon nanotube / carbon nitride modified electrode, which is an electrochemical sensor for detecting acetaminophen.
The use of the electrochemical sensor according to claim 6 in detecting acetaminophen.
A detection method for detecting acetaminophen, characterized in that the method includes the detection step using the electrochemical sensor according to claim 6.
The method according to claim 9, characterized in that: (1) the preparation of a standard solution: preparing a set of acetaminophen standard solutions with different concentrations;

(2) Drawing of working curve: place the electrochemical sensor of claim 6 in the acetaminophen standard solution of different concentrations to perform square wave voltammetry to obtain the response peaks of the acetaminophen standard solution of different concentrations Current, and then draw a linear relationship curve according to the concentration of acetaminophen standard solution and the response peak current;

(3) Detection of the sample: place the electrochemical sensor according to claim 6 in the sample to be tested for square wave voltammetry to obtain the response peak current of the sample to be tested, and then obtain the test to be tested according to the linear relationship curve The concentration of acetaminophen in the sample.