WO2020082418A1 - 避免气泡干扰的浮游生物沉降器 - Google Patents

避免气泡干扰的浮游生物沉降器 Download PDF

Info

Publication number
WO2020082418A1
WO2020082418A1 PCT/CN2018/114118 CN2018114118W WO2020082418A1 WO 2020082418 A1 WO2020082418 A1 WO 2020082418A1 CN 2018114118 W CN2018114118 W CN 2018114118W WO 2020082418 A1 WO2020082418 A1 WO 2020082418A1
Authority
WO
WIPO (PCT)
Prior art keywords
base
cover glass
column
plankton
settlement
Prior art date
Application number
PCT/CN2018/114118
Other languages
English (en)
French (fr)
Inventor
王子羲
张晓康
甄文媛
郭越红
Original Assignee
清华大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 清华大学 filed Critical 清华大学
Publication of WO2020082418A1 publication Critical patent/WO2020082418A1/zh

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation

Definitions

  • the invention relates to the technical field of plankton sampling, in particular to a plankton settler.
  • the working method of the conventional settler is as follows:
  • the liquid to be settled and separated is injected into the space surrounded by the slide glass [6], the base [1], the settling column [2] and the settling column cover glass [3].
  • the cover glass of the settling column [3] seals the upper end of the liquid column with negative fluid pressure, and the clamping ring [7] compresses the slide glass [6] to prevent liquid leakage.
  • the liquid column uses gravity to settle. After standing for a certain period of time, press down the settling column cover glass [3] and the settling column [2], and push the settling column along the long side of the base [1]. The settling column moves with the settling supernatant.
  • the sediment cover [8] needs to be covered with a base cover glass [4]. As shown in Figure 4, after the base cover slip [4] clings to the base [1], it translates along the long side of the base towards the position of the sediment until the space where the sediment is located is completely closed, as shown in Figure 5.
  • the sedimentation liquid of the covered base coverslip [4] can be used for subsequent counting operations.
  • the present invention provides a plankton settler to avoid the interference of bubbles.
  • a side of the settlement column [2] is provided with a joint surface which is closely attached to the side of the base cover glass [4].
  • the settling column [2] and the base cover glass [4] can be close to each other on the side, while the end face is close to the upper surface of the base [1] and slides along the long side of the base [1].
  • the wettability and surface tension of the sealing liquid are used to prevent air from entering these joint surfaces.
  • the beneficial effect of the present invention is that, when the settler separates the supernatant liquid and the settled liquid, the settled liquid is never in contact with air. Therefore, the process will not form bubbles in the sedimentation liquid, and the sedimentation liquid will not be contaminated by outside. This operation process is simple and reliable.
  • FIG 1 is an external view of the plankton settler to avoid the interference of air bubbles according to the present invention
  • Figure 2 is a structural diagram of the existing plankton settler when it is in a settled state
  • Figure 3 is a structural diagram of the existing plankton settler when the supernatant is removed
  • FIG. 4 is a structural diagram of the existing plankton settler after removing the supernatant, the sediment is not covered with the base cover glass;
  • FIG. 5 is a structure diagram of the existing plankton settler after the supernatant is removed, and the sediment cover is covered with a base cover glass;
  • FIG. 6 is a structural diagram of the settlement column of the present invention when the supernatant is removed, the settlement column and the cover glass of the base are close to each other and slide along the base;
  • FIG. 7 is a structural diagram of the base cover glass covering the sediment after removing the supernatant of the settler of the present invention.
  • the side of the settling column [2] and the side of the base cover glass [4] are designed with a joint surface with a length greater than the diameter of the upper surface of the settling liquid [8]. Ensure that during the process of removing the supernatant [9], the upper surface of the settling liquid [8] is always settled by the settling column [2]-settling column [2] / base cover glass [4]-base cover glass [ 4] Cover.
  • the joint surface between the settling column [2] and the base cover glass [4] can be designed as a rectangular plane perpendicular to the fitting surface of the settling column [2] / base [1], or it can be designed as a settling column [2] ] / Base [1]
  • the rectangular inclined plane with a certain inclination angle on the bonding surface can be designed as a rectangular plane perpendicular to the fitting surface of the settling column [2] / base [1], or it can be designed as a settling column [2] ] / Base [1]
  • the rectangular inclined plane with a certain inclination angle on the bonding surface.
  • the sealing liquid can be distilled water, supernatant [9], grease, paraffin or other fluids that will not affect the detection performance of the sediment [8].
  • Settlement column [2], base cover glass [4], base [1] are made of materials that have good wettability with water.
  • the settling column [2] uses transparent engineering plastics such as polymethyl methacrylate (PMMA) or polycarbonate (PC), the base cover glass [4] uses quartz glass or ordinary glass, and the base [1] uses titanium, Titanium alloy or stainless steel material.
  • PMMA polymethyl methacrylate
  • PC polycarbonate
  • the base cover glass [4] uses quartz glass or ordinary glass
  • the base [1] uses titanium, Titanium alloy or stainless steel material.
  • the selection of other components is the same as that of the conventional settler.
  • the above-mentioned bonding surface and the bonding surface need to be kept flat during molding.
  • a guide plane is designed on the base [1] to restrict the sliding direction of the settlement column [2] and the base cover glass [4].
  • the guide constraint surface can also be realized by introducing external parts.
  • the settling operation of the settler of the present invention is the same as that of the traditional settler. See 2 for the situation.
  • the operation of the settler of the present invention is to remove the supernatant [9] and cover the base cover glass [4] with the settler [8].
  • the settling column is fixed, and a dropper is used to pre-coat the sealing liquid at the junction and bonding surfaces of each knot. After that, move the base cover glass [4] to adhere to the joint surface of the settling column [2] / base cover glass [4], and at the same time to the base cover glass [4] // base [1] bonding surface.
  • the settling column [2] / base cover glass [4] joint surface and base guide surface are designed, and the sealing liquid is introduced, and the joint surface and the bonding surface are extruded using the wettability of the material and the surface tension of the liquid The air inside. It is ensured that during the process of removing the supernatant of the settler, the settler liquid is always in a closed state, avoiding the mixing of air bubbles. At the same time, the operation is simple and the performance is reliable, which reduces the difficulty of training the operators and improves the success rate of sample production.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Extraction Or Liquid Replacement (AREA)

Abstract

一种避免气泡干扰的浮游生物沉降器。在沉降柱侧面，靠近沉降柱与底座贴合面处，设有一个平面，该平面与底座盖玻片的侧面形状可以相互贴合，形成接合面。沉降柱和底座盖玻片可以相互侧面贴紧地，同时端面贴紧底座上表面，沿着底座长边方向滑动。通过这种改进，使沉降器在移除上清液和覆盖盖玻片的过程中，沉降液与外部空气隔绝，从而避免了沉降液样本内混入气泡。

Description

避免气泡干扰的浮游生物沉降器 技术领域
本发明涉及浮游生物采样技术领域，特别是涉及一种浮游生物沉降器。
背景技术
为了便于对浮游生物进行计数，经常需要对采集的样本进行富集。重力沉降是浮游生物富集的常用方式。
常规的沉降器的工作方式如下：
如图2所示，待沉降分离的液体注入载玻片[6]、底座[1]、沉降柱[2]和沉降柱盖玻片[3]所包围的空间内。沉降柱盖玻片[3]利用流体负压密封住液柱上端，卡环[7]压紧载玻片[6]，防止液体泄漏。液柱利用重力作用完成沉降。静置特定时间后，向下压住沉降柱盖玻片[3]和沉降柱[2]，沿着底座[1]长边方向推动沉降柱，沉降柱连同沉降后的上清液一同移动，当沉降柱[2]移动到底板[1]上开设的漏水孔时，从沉降柱[2]上口移除沉降柱盖玻片[3]，上清液通过漏水孔流入下方的漏水槽[5]。如图3所示，留下的沉降液[8]留在载玻片[6]和底座[1]形成的空间内。
沉降液[8]上面需要盖上底座盖玻片[4]。如图4所示，底座盖玻片[4]贴紧底座[1]后，沿着底座长边向沉降液所在位置平移，直到完全封闭沉降液所在的空间，如图5所示。加盖的底座盖玻片[4]的沉降液可以进行后续的计数操作。
目前存在的问题是，在移走沉降柱[2]后，由于表面张力的作用，沉降液[8]的上液面不是水平的。图4中，当底座盖玻片[4]贴紧底座[1]平移覆盖沉降液[8]时，气体会进入底座盖玻片[4]下方，在完全封闭沉降液后，在沉降液内形成气泡。该气泡将会影响沉降液的计数。
发明内容
为了克服现有浮游生物沉降器沉降液易于混入气泡的不足，本发明提供了一种避免气泡干扰的浮游生物沉降器。
本发明所采用的技术方案是：在沉降柱[2]侧面设有一个与底座盖玻片[4]侧面紧密贴合的接合面。沉降柱[2]和底座盖玻片[4]可以相互侧面贴紧地，同时端面贴紧底座[1]上表面，沿着底座[1]长边方向滑动。在沉降柱[2]与底座盖玻片[4]的接合面上，以及底座盖玻片[4]下平面(下端面)与底座[1]接合面之间，预先注入满高浸润性的封闭液。利用封闭液的润湿性和表面张力，防止空气进入这些接合面中。如图6所示，一起沿着底座[1]长边方向推动沉降柱[2]和底座盖玻片[4]，在移走上清液[9]的同时，将沉降液[8]留在底座盖玻片[4]、沉降柱[2]、底座[1]的沉降孔，以及载玻片[6]之间形成的封闭腔体内(如图6所示)；继续推动沉降柱[2]和底座盖玻片[4]，直到沉降液[8]上表面完全被底座盖玻片[4]所覆盖(如图7所示)，再此过程中，当沉降柱[2]移动到底板[1]上开设的漏水孔时，从沉降柱[2]上口移除沉降柱盖玻片[3]，上清液通过漏水孔流入下方的漏水槽[5]。在这个移除上清液[9]的过程中，沉降液[8]与空气完全不接触，也就避免了封 装好的沉降液[8]中形成气泡。
与现有技术相比，本发明的有益效果是，在沉降器分离上清液和沉降液时，沉降液始终不与空气接触。因此，该过程在沉降液内不会形成气泡，且沉降液不会受到外来的污染，这个操作过程简单可靠。
附图说明
图1为本发明避免气泡干扰的浮游生物沉降器的外观图；
图2为现有浮游生物沉降器处于沉降状态时的结构图；
图3为现有浮游生物沉降器在排除上清液时的结构图；
图4为现有浮游生物沉降器在排除上清液后，沉降液没有盖上底座盖玻片时的结构图；
图5为现有浮游生物沉降器在排除上清液后，沉降液盖上底座盖玻片后的结构图；
图6为本发明沉降器移除上清液时，沉降柱和底座盖玻片相互侧面贴紧，沿着底座滑动时的结构图；
图7为本发明沉降器移除上清液后，底座盖玻片覆盖沉降液的结构图；
具体实施方式
下面结合附图对本发明进一步说明。
本发明的避免气泡干扰的浮游生物沉降器中，沉降柱[2]侧面与底座盖玻片[4]的侧面设计一个长度大于沉降液[8]上表面直径的接合 面，该接合面用于保障沉降器在移除上清液[9]的过程中，沉降液[8]上表面始终被沉降柱[2]-沉降柱[2]/底座盖玻片[4]-底座盖玻片[4]覆盖。沉降柱[2]与底座盖玻片[4]之间的接合面，可以设计成垂直于沉降柱[2]/底座[1]贴合面的矩形平面，也可以设计成与沉降柱[2]/底座[1]贴合面呈一定倾斜角度的矩形斜面。
沉降柱[2]/底座盖玻片[4]接合面、沉降柱[2]/底座[1]贴合面、以及底座盖玻片[4]//底座[1]贴合面，这三个接合/贴合面，需要能够同时贴紧。同时贴紧后，通过注入高浸润性的封闭液，将贴合面内的空气挤出。封闭液可以选用蒸馏水、上清液[9]、油脂、石蜡或者其它不会影响沉降液[8]检测性能的流体。沉降柱[2]、底座盖玻片[4]、底座[1]的材质，选用与水具有良好润湿性的材料。例如，沉降柱[2]采用聚甲基丙烯酸甲酯(PMMA)或者聚碳酸酯(PC)等透明工程塑料，底座盖玻片[4]采用石英玻璃或者普通玻璃，底座[1]采用钛、钛合金或者不锈钢材料。其它部件的选材，与常规沉降器相同。
为了保障上述贴合面在静止和滑动时的密实性，上述贴合面和接合面在成型制作时需要保持平整。
当沉降柱[2]和底座盖玻片[4]在底座贴合面上滑动时，为了保障贴合面的密实性，防止出现上清液[9]或者沉降液[8]意外漏气的情况，在底座[1]上设计了导向平面，用于约束沉降柱[2]和底座盖玻片[4]的滑动方向。该导向约束面，也可以通过引入外部零件来实现。
本发明的沉降器在沉降操作与传统沉降器相同，情形见如2。本发明的沉降器在去除上清液[9]以及给沉降液[8]覆盖底座盖玻片[4] 的操作是同时完成的。操作时。完成沉降的沉降柱固定不动，用滴液管在各个结接合面和贴合面处，预涂封闭液。之后，移动底座盖玻片[4]，贴紧沉降柱[2]/底座盖玻片[4]接合面，同时贴紧底座盖玻片[4]//底座[1]贴合面。贴紧后，用滴液管在贴合缝隙边缘注封闭液，赶出贴合面内的空气。之后，固定底座[1]不动，用手按压底座盖玻片[4]和沉降柱[2]，同时推动底座盖玻片[4]和沉降柱[2]沿着底座[1]长边贴合滑动(见图6)，直到滑动到底座盖玻片[4]完全覆盖沉降液[8]的上表面。此时，打开沉降柱盖玻片[3](见图7)，上清液通过漏水孔流入下方的漏水槽[5]。移除沉降柱[2]后，形成如图5所示的可以用于进一步检测的沉降液样本。
本发明中设计了沉降柱[2]/底座盖玻片[4]接合面和底座导向面，且引入了封闭液，利用材料的润湿性和液体表面张力挤出了接合面和贴合面内的空气。保障了沉降器在移除上清液的过程中，沉降液始终处于封闭状态，避免了气泡混入。同时操作简单，性能可靠，降低了随操作人员的培训难度，提高了样本制作成功率。

Claims (4)

  1. 一种浮游生物沉降器，包含底座[1]、卡环[7]、载玻片[6]、漏水槽[5]、沉降柱[2],沉降柱盖玻片[3],底座盖玻片[4]。其特征在于：在沉降柱[2]侧面，在靠近沉降柱[2]与底座[1]贴合面处，设有一个平面，该平面与底座盖玻片[4]的侧面形状可以相互贴合，形成接合面，且接合面长度大于沉降液[8]的上表面直径。沉降柱[2]和底座盖玻片[4]可以相互侧面贴紧地，同时端面贴紧底座[1]上表面，沿着底座[1]长边方向滑动。
  2. 根据权利要求1所述的浮游生物沉降器，其特征在于：在沉降柱[2]与底座盖玻片[4]的接合面上，以及底座盖玻片[4]下平面(下端面)与底座[1]接合面之间，预先注入满高浸润性的封闭液。
  3. 根据权利要求1所述的浮游生物沉降器，其特征在于：在底座[1]上面，沿着其长边方向，有一个导向立板，用于沉降柱[2]和底座盖玻片[4]在一起推动时，起到导向作用。
  4. 根据权利要求1所述的浮游生物沉降器，其特征在于：底座[1]和卡环[7]采用钛或者钛合金材质制作。
PCT/CN2018/114118 2018-10-24 2018-11-06 避免气泡干扰的浮游生物沉降器 WO2020082418A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201810671923.8 2018-10-24
CN201810671923.8A CN109001017A (zh) 2018-10-24 2018-10-24 避免气泡干扰的浮游生物沉降器

Publications (1)

Publication Number Publication Date
WO2020082418A1 true WO2020082418A1 (zh) 2020-04-30

Family

ID=64602034

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2018/114118 WO2020082418A1 (zh) 2018-10-24 2018-11-06 避免气泡干扰的浮游生物沉降器

Country Status (2)

Country Link
CN (1) CN109001017A (zh)
WO (1) WO2020082418A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114247551B (zh) * 2021-12-15 2023-06-06 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) 浮游动物分离方法及分离装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101019085B1 (ko) * 2010-10-19 2011-03-07 (주)패스텍 세포 채취용 필터 용기
CN204085973U (zh) * 2014-08-11 2015-01-07 孝感市亚光医用电子技术有限公司 一种快速沉降制片装置
CN205301026U (zh) * 2015-12-08 2016-06-08 长沙远安生物科技有限公司 一种液基薄层细胞的细胞涂片用制片仓
CN107376436A (zh) * 2017-08-15 2017-11-24 长江流域水环境监测中心 一种浮游植物沉降分离装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201434798Y (zh) * 2009-06-02 2010-03-31 上海海事大学 一种改进型Utermhl计数装置
CN203759745U (zh) * 2014-04-10 2014-08-06 尹翠玲 浮游植物的计数装置及系统
DE102015105306A1 (de) * 2015-04-08 2016-10-13 HYDRO - BIOS Apparatebau Gesellschaft mit beschränkter Haftung Probenentnahmevorrichtung und Reihenanordnung daraus
CN205079992U (zh) * 2015-10-23 2016-03-09 厦门迈威生物科技有限公司 一种离心式液基细胞制片的加液装置
CN106124266B (zh) * 2016-06-22 2019-10-18 杭州海世嘉生物科技有限公司 一种液基细胞制片方法
CN106483005B (zh) * 2016-10-24 2019-04-16 中国水产科学研究院长江水产研究所 一种用于浮游植物、原生动物定量样品鉴定的封片方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101019085B1 (ko) * 2010-10-19 2011-03-07 (주)패스텍 세포 채취용 필터 용기
CN204085973U (zh) * 2014-08-11 2015-01-07 孝感市亚光医用电子技术有限公司 一种快速沉降制片装置
CN205301026U (zh) * 2015-12-08 2016-06-08 长沙远安生物科技有限公司 一种液基薄层细胞的细胞涂片用制片仓
CN107376436A (zh) * 2017-08-15 2017-11-24 长江流域水环境监测中心 一种浮游植物沉降分离装置

Also Published As

Publication number Publication date
CN109001017A (zh) 2018-12-14

Similar Documents

Publication Publication Date Title
CN105013550B (zh) 微流控芯片夹具和微流控芯片
CN204694494U (zh) 一种可拆卸浅水沉积物原位柱状样采样装置
WO2020082418A1 (zh) 避免气泡干扰的浮游生物沉降器
CN205452425U (zh) 注液装置及其注液针与注液杯密封结构
CN205182250U (zh) 一种用于悬浮分散液液微萃取的装置
CN205808905U (zh) 一种具有排气功能的计数池
CN210775357U (zh) 一种液相色谱仪用进样瓶
CN201269831Y (zh) 流动溶液集合取样装置
CN206725290U (zh) 一种收集管及小便采样器
CN209757959U (zh) 一种流式细胞仪料液桶密封结构
SE328130B (zh)
CN212513805U (zh) 一种液体取样器
CN207587693U (zh) 一种同位素质谱仪的准确取样和进样装置
CN205352758U (zh) 手提式水质采样器
CN107596492A (zh) 一种输液器防回流装置
CN208206548U (zh) 一种新型采水器
CN209366854U (zh) 密封液体盖
CN208642003U (zh) 一种便于组装的实验室用多联过滤器
CN214310040U (zh) 一种具有高效密封性的血沉测试管
CN207776831U (zh) 一种油管防污防溅导流器
CN206862694U (zh) 用于四氯化硅的取样阀
CN219210005U (zh) 一种移液枪头装置
CN205386367U (zh) 液体过滤装置
CN206187640U (zh) 一种流体密封组件及采样装置
CN221883130U (zh) 一种适用于蓝藻爆发期的地表水采样工具

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18938198

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18938198

Country of ref document: EP

Kind code of ref document: A1