WO2020073946A1 - Compositions contenant des bactériophages et leurs utilisations pour le traitement ou la prévention des lésions cutanées et du psoriasis - Google Patents

Compositions contenant des bactériophages et leurs utilisations pour le traitement ou la prévention des lésions cutanées et du psoriasis Download PDF

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WO2020073946A1
WO2020073946A1 PCT/CN2019/110309 CN2019110309W WO2020073946A1 WO 2020073946 A1 WO2020073946 A1 WO 2020073946A1 CN 2019110309 W CN2019110309 W CN 2019110309W WO 2020073946 A1 WO2020073946 A1 WO 2020073946A1
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phage
pseudomonas
composition
skin
virus
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PCT/CN2019/110309
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English (en)
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Herbert Hei Man PANG
Hailun WANG
Hin Lee Henry CHAN
Michael Yuxuan NI
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The University Of Hong Kong
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00032—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present disclosure relates to phage therapy for the treatment and control of psoriasis, skin lesions and infections. More particularly, the present disclosure relates to a composition comprising bacteriophage and uses thereof. Provided herein is a method of making the composition comprising one or more strains of bacteriophage. Also provided is a method of treating and preventing a skin disorder such as psoriasis or skin lesions. Also provided herein is a dermatological composition comprising one or more strains of bacteriophage and cosmetic use thereof.
  • Psoriasis is a multifaceted immune-mediated skin disease and has been associated with poorer quality of life, increased health care burden, and other comorbidities (American Academy of Dermatology Work Group et al. 2011) .
  • the number of subjects with psoriasis is estimated to be 125 to 250 million people (Armstrong 2017) .
  • Psoriasis patients have an increased risk of developing psoriatic arthritis that further reduce their quality of life (Alinagphi et al. 2018) .
  • WHO member states recognized psoriasis as a serious disease.
  • Recent biologics treatment developed for psoriasis has improved prognosis for some patients but the current pricing can restrict their use (Al Sawah et al. 2017) .
  • psoriasis continues to have public health impact to the society (Helmick et al. 2013) . Symptoms of psoriasis are not only physically debilitating, they also have psychological effects on patients (Clausen et al 2017, Randa et al. 2018) . In addition, infections of psoriatic skins are usually treated by antibiotics, however antibiotic resistance can result from such treatments. This creates problem for the patients if their infections become resistance to antibiotic treatments.
  • bacteriophage-based probiotic with or without bacteria-based probiotics.
  • a composition comprising bacteriophages for phage-based therapies to treat psoriasis, skin lesions and infections.
  • the therapy is useful for suppressing unhealthy bacteria on psoriatic skins which then lead to healthier skin.
  • phage-based treatments for infections which reduces the risk of antimicrobial resistance.
  • the present disclosure provides bacteriophage therapy that enables the restoration of the equilibrium of the microbiome in treated lesion areas.
  • the present disclosure also discloses a skin care product for cosmetic use that restore and maintain the condition of healthy skin in a subject.
  • composition comprising one or more bacteriophage strains selected from the group consisting of Acinetobacter phage Presley, Salmonella phage vB_SenS-Ent2, Bacillus phage SP-10, Pseudomonas phage O4, Haemophilus phage Aaphi23, Enterobacteria phage SfI, Bordetella virus BPP1, Mycobacterium phage DrDrey, Burkholderia virus BcepC6B, Rhodoferax phage P26218, Burkholderia virus phi1026b, Idiomarinaceae phage Phi1M2-2, Ralstonia phage RSK1, Sulfitobacter phage NYA-2014a, Thalassomonas phage BA3, Pseudomonas phage KPP25, Pseudomonas phage NP1, and Pseudomonas virus F116 or a variant thereof
  • composition comprising one or more bacteriophage strains selected from the group consisting of Acinetobacter phage Presley, Salmonella phage vB_SenS-Ent2, Bacillus phage SP-10, Pseudomonas phage O4, Haemophilus phage Aaphi23, Enterobacteria phage SfI, Bordetella virus BPP1, Mycobacterium phage DrDrey, Burkholderia virus BcepC6B, Rhodoferax phage P26218, Burkholderia virus phi1026b, Idiomarinaceae phage Phi1M2-2, Ralstonia phage RSK1, Sulfitobacter phage NYA-2014a, Thalassomonas phage BA3, Pseudomonas phage KPP25, Pseudomonas phage NP1, and Pseudomonas virus F116, or a variant
  • composition wherein the bacteriophage shows antibacterial activity against at least one of Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia or Rhodoferax.
  • composition comprising one or more bacteriophage strains selected from Acinetobacter phage Presley, Pseudomonas phage O4, or a variant thereof, said variant having at least 50%sequence coverage and 70 %sequence identity to SEQ ID NO: 01 and 02 (sequence information provided in Supplementary Appendix) , respectively.
  • composition wherein the bacteriophage shows antibacterial activity against one or more bacterial strains selected from Acinetobacter, or Pseudomonas.
  • composition comprising one or more bacteriophage strains selected from Acinetobacter phage Presley, Pseudomonas phage O4, or a variant thereof, said variant having at least 50%sequence coverage and 70 %sequence identity to SEQ ID NO: 01 and 02 (sequence information provided in Supplementary Appendix) , respectively.
  • composition comprising bacteriophage that shows antibacterial activity against one or more strains selected from Acinetobacter or Pseudomonas.
  • composition comprising bacteriophages; and a pharmaceutically acceptable carrier.
  • the composition is a dermatological composition.
  • the pharmaceutical composition further comprises an antibiotic agent and/or an agent for treating psoriasis.
  • Also provided herein is a method of treating or preventing psoriasis in a subject comprising administering to the subject a therapeutically effective amount of the pharmaceutical composition.
  • the psoriasis is associated with an area of intact skin. In certain embodiments, the psoriasis is associated with an area of non-intact skin.
  • the pharmaceutical composition is administered topically to the subject. In certain embodiments, the pharmaceutical composition is administered to the subject’s elbow, forearm, knee and scalp.
  • the pharmaceutical composition is administered to a subject at a skin area with lesion.
  • a method of cosmetic treatment comprising applying a dermatological composition on a subject.
  • a method for improving condition of a subject’s skin by modifying the microbiome in the subject comprising contacting the subject’s skin with a composition disclosed herein.
  • the dermatological composition is administered to a subject at a skin area without lesion.
  • the dermatological composition is administered to a subject at a skin area with lesion.
  • a method for preparing a composition comprising producing at least one bacteriophage strain selected from the group consisting of Acinetobacter phage Presley, Salmonella phage vB_SenS-Ent2, Bacillus phage SP-10, Pseudomonas phage O4, Haemophilus phage Aaphi23, Enterobacteria phage SfI, Bordetella virus BPP1, Mycobacterium phage DrDrey, Burkholderia virus BcepC6B, Rhodoferax phage P26218, Burkholderia virus phi1026b, Idiomarinaceae phage Phi1M2-2, Ralstonia phage RSK1, Sulfitobacter phage NYA-2014a, Thalassomonas phage BA3, Pseudomonas phage KPP25, Pseudomonas phage NP1, and Pseudomonas virus F
  • psoriasis and skin lesion are characterized by a microbiome of low diversity, comprising predominantly bacteria of the Pseudomonas species, Acinetobacter species, and Cutibacterium acnes species than in the microbiome of non-lesion areas and/or health controls.
  • step b) comparing the measurement obtained from step a) to a control
  • FIG. 1 shows distribution of most abundant species in phage community and bacterial community across different samples.
  • FIG. 2 shows comparison of Beta Diversity PCoA1 for Phage and Bacterial Species community Between Lesional Skin and Healthy Skin.
  • FIG. 3 shows abundance between lesional skin and healthy skin for top ten most abundant phage species.
  • FIG. 4 shows comparison of different levels of phage abundance to the host bacterial abundance.
  • FIG. 5 Order of Top Ten Most Abundant Phage Species.
  • FIG. 7. P values of Statistical Tests for Top Ten Most Abundant Phages.
  • FIG. 8 Comparison of Beta Diversity PCoA1 for Phage (A) and Bacterial (B) Species Community Between Two Groups of Sex.
  • FIG. 9A PCoA Plot Illustrating Differences of Phage Species Composition Between Female and Male.
  • FIG. 9B PCoA Plot Illustrating Differences of Bacterial Species Composition Between Female and Male.
  • FIG. 10 Comparison of Alpha Diversity for Phage (A) and Bacterial (B) Species Between Lesional Skin and Healthy Skin.
  • FIG. 11A PCoA Plot Illustrating Differences of Phage Species Composition Between Lesional Skin and Healthy Skin.
  • FIG. 11B PCoA Plot Illustrating Differences of Bacterial Species Composition Between Lesional Skin and Healthy Skin.
  • FIG. 12 Order of Top Ten Most Abundant Category Two Phage Species.
  • FIG. 13 Differential abundance between lesional skin and healthy skin for dominate category two phage species.
  • FIG. 14 Suggested minimum starting %level of Potential Probiotics Phage Species.
  • skin herein encompasses both the skin and the scalp, but does not comprise mucous membranes.
  • microbiome means all genomes of bacteria present at the surface of a skin area.
  • lesion area means an area of the skin affected by a disease or disorder.
  • the lesion areas are in particular characterized by skin dryness, redness, blisters, scabs or combinations thereof.
  • non-lesion area refers to an area of the skin not affected by a skin disease or disorder, and preferably not affected by any other pathology or cutaneous wound.
  • the area of a sample generally has a surface with diameter of 0.5-2 cm.
  • the non-lesion area is a healthy area that is on the contralateral skin site to the lesion area.
  • antibiotics refers to compounds that either kill or inhibit the growth of bacteria.
  • isolated with respect to a bacteriophage means that the phage has been measurably increased in concentration by any purification process, including but not limited to, isolation from the environment or culture, e.g., isolation from culture following propagation and/or amplification, centrifugation, etc., thereby partially, substantially, nearly completely, or completely removing impurities, such as host cells and host cell components.
  • an isolated phage meant for use in therapeutic compositions intended for administration to humans ordinarily must be of high purity in accordance with regulatory standards and good manufacturing processes.
  • variant in the context of nucleic acid sequences (e.g. from Genbank database) refers to a nucleic acid sequence that comprises or consists of a nucleic acid sequence having a sequence identity of at least 80-85%, 85-90%, 90-93%, 93-95%, 95-96%, 96-97%, 97-98%, 98-99%, 99-99.5%or more with a reference nucleic acid sequence.
  • a variant may be selected that maintains one or more function of the reference nucleic acid sequence.
  • a variant bacteriophage may exhibit at least one biological activity, e.g., antibacterial activity (e.g., lytic killing activity) , of the bacteriophage from which it is derived.
  • cover refers to the percentage of a query sequence represented in the alignment with the reference sequence.
  • the term "in combination” refers to the use of an additional prophylactic and/or therapeutic agent as well as a composition comprising combination of different strains.
  • the use of the term “in combination” does not restrict the order in which prophylactic and/or therapeutic agents are administered to a subject.
  • a first prophylactic or therapeutic agent can be administered prior to (e.g., 5 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hour, 2 hours, 4 hours, 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 96 hours, 1 week, 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 8 weeks, or 12 weeks before) , concomitantly with, or subsequent to (e.g., 5 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hour, 2 hours, 4 hours, 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 96 hours, 1 week, 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 8 weeks, or 12 weeks after) the administration of a second prophylactic or therapeutic agent (different from the first prophylactic or therapeutic agent) to a subject.
  • a second prophylactic or therapeutic agent different from the first prophylactic or therapeutic agent
  • prolactic agent and “prophylactic agents” refer to an agent, such as a bacteriophage composition of the invention, which can be used in the prevention, management, or control of one or more symptoms of a disease or disorder, such as but not limited to, psoriasis or skin lesion.
  • therapeutic agent and “therapeutic agents” refer to an agent, such as a bacteriophage composition, that can be used in the treatment, management, or control of one or more symptoms of a disease or disorder, in particular, a disease or disorder.
  • the terms “treat” , “treatment” and “treating” refer to obtaining a therapeutic benefit in a subject receiving a pharmaceutical or dermatological composition.
  • the object is to eliminate, lessen, decrease the severity of, ameliorate, or slow the progression of the symptoms or underlying cause (e.g., bacterial infection) associated with the pathological condition or disorder.
  • a “therapeutically effective amount” refers to that amount of a therapeutic agent, such as a pharmaceutical composition of the invention, sufficient to achieve at least one therapeutic benefit in a subject receiving the pharmaceutical composition.
  • the terms “prevent” , “prevention” and “preventing” refer to obtaining a prophylactic benefit in a subject receiving a pharmaceutical or dermatological composition.
  • the object is to delay or prevent the symptoms or underlying cause (e.g., psoriasis, skin lesion or bacterial infection) associated with the pathological condition or disorder.
  • a “prophylactically effective amount” refers to that amount of a prophylactic agent, such as a pharmaceutical or dermatological composition disclosed herein, sufficient to achieve at least one prophylactic benefit in a subject receiving the pharmaceutical or dermatological composition.
  • antibacterial activity and “antimicrobial activity” , with reference to a bacteriophage (or variant thereof) or bacteriophage product, are used interchangeably to refer to the ability to kill and/or inhibit the growth or reproduction of a microorganism, in particular, the bacteria of the species or strain that the bacteriophage infects.
  • antibacterial or antimicrobial activity is assessed by culturing bacteria, e.g., Gram-positive bacteria, Gram-negative bacteria or bacteria not classified as either Gram-positive or Gram-negative, according to standard techniques (e.g., in liquid culture or on agar plates) , contacting the culture with a bacteriophage or variant thereof of the invention and monitoring cell growth after said contacting.
  • the bacteria in a liquid culture, may be grown to an optical density ( "OD" ) representative of a mid-point in exponential growth of the culture; the culture is exposed to one or more concentrations of one or more bacteriophage of the invention, or variants thereof, and the OD is monitored relative to a control culture. Decreased OD relative to a control culture is representative of a bacteriophage exhibiting antibacterial activity (e.g., exhibits lytic killing activity) .
  • bacterial colonies can be allowed to form on an agar plate, the plate exposed to one or more bacteriophage of the invention, or variants thereof, and subsequent growth of the colonies evaluated related to control plates. Decreased size of colonies, or decreased total numbers of colonies, indicate a bacteriophage with antibacterial activity.
  • the term “synergistic effect” refers to the interaction between two or more agents when the combined effect is larger than the sum of the effects of the individual components.
  • subject and “patient” are used interchangeably herein.
  • the terms “subject” and “subjects” refer to an animal, such as a mammal including a non-primate (e.g., a cow, pig, horse, cat, dog, rat, and mouse) and a primate (e.g., a monkey such as a cynomolgous monkey, a chimpanzee and a human) ., and for example, a human.
  • a non-primate e.g., a cow, pig, horse, cat, dog, rat, and mouse
  • a primate e.g., a monkey such as a cynomolgous monkey, a chimpanzee and a human
  • a human e.g., a monkey such as a cynomolgous monkey, a chimpanzee and a human
  • terapéuticaally effective amount includes an amount of an agent or composition that, when administered to a subject for treatment, is sufficient to effect such treatment.
  • a “therapeutically effective amount” can vary depending on, inter alia, the compound, the infection and its severity, and the age, weight, etc., of the subject to be treated.
  • resistant when refer to bacteria or bacterial infections that are no longer responsive to an agent that was previously effective in reducing the bacterial growth or preventing any increase in bacterial growth.
  • the present disclosure is directed to phage therapy for the treatment and control of skin condition including psoriasis, skin lesion and bacterial infections.
  • the invention relates to novel compositions of different bacteriophage strains.
  • the composition may comprise at least two different isolated strains of bacteriophage, for example, two, three, four, five, six, seven, eight, nine, ten, or more different isolated bacteriophage strains.
  • the composition may be used alone or in further combination with other therapies, e.g., antibiotic agents or probiotic agents.
  • Composition comprising different strains of bacteriophage provide advantages to the use of phages individually, e.g., to increase the lytic activity against a particular bacterial strain and/or to decrease the possibility of emergence of bacteria resistant to an individual bacteriophage.
  • Different bacteriophage can be mixed in a composition to broaden their properties, preferably resulting in a collectively greater antibacterial spectrum of activity.
  • few phage composition exist with antimicrobial activity against different bacteria, probably because of the difficulty in combining different specificities of bacteriophage strains, while maintaining infecting ability and/or lytic activity of the individual bacteriophage in the presence of distinct bacteriophage strains.
  • the disclosure provides a composition comprising at least one isolated bacteriophage strains, including but not limited to, phage Acinetobacter phage Presley, Salmonella phage vB_SenS-Ent2, Bacillus phage SP-10, Pseudomonas phage O4, Haemophilus phage Aaphi23, Enterobacteria phage SfI, Bordetella virus BPP1, Mycobacterium phage DrDrey, Burkholderia virus BcepC6B, Rhodoferax phage P26218, or a combination thereof.
  • isolated bacteriophage strains including but not limited to, phage Acinetobacter phage Presley, Salmonella phage vB_SenS-Ent2, Bacillus phage SP-10, Pseudomonas phage O4, Haemophilus phage Aaphi23, Enterobacteria phage SfI, Bordetella virus BPP1, Myco
  • the composition is formulated with at least two, three, four, five, six, seven, eight, nine, ten, eleven, twelve different isolated bacteriophage strains. In certain embodiments, the composition is a topical formulation. In certain embodiments, the composition is useful for the treatment and/or prevention of psoriasis and bacterial infections.
  • the composition comprises at least two different isolated bacteriophage strains, with antibacterial activity against the same or different bacterial strains.
  • the therapeutic components of the composition target two or more strains of bacteria.
  • the composition comprises 2 phage strains, 3 phage strains, 4 phage strains, 5 phage strains, 6 phage strains, 7 phage strains, 8 phage stains, 9 phage strains, 10 phage strains.
  • the composition comprises 2-4 phage strains, 4-6 phage strains, 6-8 phage strains, 8-10 phage strains, or 10-12 phage strains.
  • the combination does not impair or reduce (or does not substantially or significantly impair or reduce) infecting ability and/or lytic activity of the individual bacteriophage in the presence of distinct bacteriophage strains.
  • At least one phage strain of the composition is a strain with antibacterial activity against at least one Gram-negative bacterium, including but not limited to Acinetobacter and Pseudomonas; and/or against at least one Gram-positive bacteria including but not limited to Bacillus.
  • the composition comprises at least two different isolated bacteriophage strains where the strains show antibacterial activity against at least one of Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof.
  • the invention provides a composition comprising one or more strains of bacteriophage having a genome comprising or consisting of the nucleic acid sequence of SEQ ID NO: 01 and 02 (sequence information provided in Supplementary Appendix) , which bacteriophage exhibits at least one biological activity, e.g., antimicrobial or antibacterial activity (e.g., lytic killing activity) .
  • bacteriophage having a genome comprising or consisting of the nucleic acid sequence of SEQ ID NO: 01 and 02 (sequence information provided in Supplementary Appendix) , which bacteriophage exhibits at least one biological activity, e.g., antimicrobial or antibacterial activity (e.g., lytic killing activity) .
  • a variant bacteriophage strain which comprises or consists of a genome having a sequence identity of at least about 80-85%, 85-90%, 90-93%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more with the nucleic acid sequence as provided in NCBI accession Nos: YP_009007647.1 (Acinetobacter phage Presley) , YP_009304497.1 (Pseudomonas phage O4) , YP_009030589.1 (Pseudomonas phage KPP25) , YP_009285843.1 (Pseudomonas phage NP1) , YP_164304.1 (Pseudomonas virus F116) , which bacteriophage exhibits at least one biological activity, e.g., antimicrobial or antibacterial activity (e.g., lytic killing activity) , of bacteri
  • Bacteriophage may be isolated from a bacterial sample using any method described herein or known in the art (see, e.g., Carlson, "Working with bacteriophage: common techniques and methodological approaches, " In, Kutter and Sulakvelidze (Eds) Bacteriophage: Biology and Applications, 5 th ed. CRC Press (2005) ; incorporated herein by reference in its entirety) . Bacteriophage also may be isolated from any other bacterial strain susceptible to infection by one or more of the bacteriophage, and in which the bacteriophage replicate.
  • compositions comprising the bacteriophage as described herein may be optionally delivered with other antibacterial agents in the form of antibacterial composition, or individually, yet close enough in time to have a synergistic effect on the treatment of the psoriasis, skin lesions or infection.
  • An antibacterial composition is a mixture of any one of the compounds described herein with another antibacterial drug.
  • a common administration vehicle e.g., tablet, implants, injectable solution, injectable liposome solution, etc.
  • the composition comprises an antibacterial agent.
  • the composition does not comprise an antibacterial agent.
  • the composition is a phage-based probiotics with bacteria-based probiotics.
  • the composition is a phage-based probiotics without bacteria-based probiotics.
  • the bacteriophage composition of the disclosure are incorporated into a pharmaceutical composition for use in the treatment and/or prevention of psoriasis, skin lesion and bacterial infections caused by bacteria including, but not limited to, Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof.
  • Different phage strains e.g., as disclosed herein, may be combined with a pharmaceutically acceptable carrier, such as an excipient or stabilizer.
  • Examples of pharmaceutically acceptable carriers, excipients, and stabilizers include, but are not limited to, buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid; low molecular weight polypeptides; proteins, such as serum albumin and gelatin; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, arginine or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugar alcohols such as mannitol or sorbitol; salt-forming counterions such as sodium; and/or nonionic surfactants such as TWEEN TM , polyethylene glycol (PEG) , and PLURONICS.
  • buffers such as phosphate, citrate, and other organic acids
  • antioxidants including ascorbic acid
  • low molecular weight polypeptides proteins, such as serum albumin
  • compositions of the present invention can also include a lubricant, a wetting agent, an emulsifier, a suspending agent, and a preservative, e.g., in addition to the above ingredients.
  • compositions of the present invention may also be combined with one or more non-phage therapeutic and/or prophylactic agents, useful for the treatment and/or prevention of bacterial infections, as described herein and/or known in the art (e.g. one or more antibiotic agents) .
  • Other therapeutic and/or prophylactic agents that may be used in combination with the composition of the invention include, but are not limited to, antibiotic agents, anti-inflammatory agents, antiviral agents, local anesthetic agents, growth factors, and corticosteroids.
  • the pharmaceutical composition is formulated for treatment and/or prevention of psoriasis, skin lesion or bacterial infections and comprises one or more additional therapeutic and/or prophylactic agents selected from antibiotic agents, local anesthetic agents, and growth factors.
  • the composition is administered in the absence of an antibiotic agent.
  • Standard antibiotics that may be used with pharmaceutical compositions comprising a bacteriophage include, but are not limited to, amikacin, gentamicin, kanamycin, neomycin, netilmicin, paromomycin, rhodostreptomycin, streptomycin, tobramycin, apramycin, rifamycin, naphthomycin, mupirocin, geldanamycin, ansamitocin, carbacephems, imipenem, meropenem, ertapenem, faropenem, doripenem, panipenem/betamipron, biapenem, PZ-601, cephalosporins, cefacetrile, cefadroxil, cefalexin, cefaloglycin, cefalonium, cefaloridine, cefalotin, cefapirin, cefatrizine, cefazaflur, cefazedone, cefazolin, cefradine
  • ceftobiprole azithromycin, clarithromycin, dirithromycin, erythromycin, roxithromycin, aztreonam, pencillin and penicillin derivatives, actinomycin, bacitracin, colistin, polymyxin B, cinoxacin, flumequine, nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, rosoxacin, ciprofloxacin, enoxacin, fleroxacin, lomefloxacin, nadifloxacin, norfloxacin, ofloxacin, pefloxacin, rufloxacin, balofloxacin, gatifloxacin, grepafloxacin, levofloxacin, moxifloxacin, pazufloxacin, sparfloxacin, temafloxacin, tosufloxacin, clinafloxacin, garenoxacin, gemifloxacin
  • Local anesthetics that may be formulated for use with pharmaceutical compositions disclosed herein include, but are not limited to, tetracaine, tetracaine hydrochloride, lidocaine hydrochloride, dimethisoquin hydrochloride, dibucaine, dibucaine hydrochloride, butambenpicrate, and pramoxine hydrochloride.
  • An exemplary concentration of local anesthetic is about 0.025%to about 5%by weight of the total composition.
  • the anesthetic agent is formulated in a pharmaceutical composition that is a topical formulation.
  • Corticosteroids that may be used with the compositions disclosed herein include, but are not limited to, betamethasone, dipropionate, fluocinolone, actinide, betamethasone valerate, triamcinolone actinide, clobetasol propionate, desoximetasone, diflorasone diacetate, amcinonide, flurandrenolide, hydrocortisone valerate, hydrocortisone butyrate, and desonide.
  • An exemplary concentration of corticosteroid is about 0.01%to about 1%by weight of the total composition.
  • the composition comprising bacteriophage may include other agents that are useful for a treating psoriasis. These agents include topical corticosteroid, retinoids, vitamin D analogues, calcipotriol, coal tar, moisturizers and emollients such as mineral oil, phototherapy, dithranol, oil, corticosteroids (i.e. desoximetasone) , fluocinonide and retinoids. In certain embodiments, the composition is used in a method in combination with phototherapy.
  • agents include topical corticosteroid, retinoids, vitamin D analogues, calcipotriol, coal tar, moisturizers and emollients such as mineral oil, phototherapy, dithranol, oil, corticosteroids (i.e. desoximetasone) , fluocinonide and retinoids.
  • the composition is used in a method in combination with phototherapy.
  • compositions comprising a bacteriophage can be formulated in a unit dose or multi-dose formulation.
  • the formulations can be topically applied, e.g., formulations selected from ointments, solutions, and sprays.
  • suitable formulations include suspensions, emulsions, extracts, powders, or granules; and additionally may include a dispersing agent or a stabilizing agent.
  • compositions disclosed herein are administered topically (e.g., in the form of a lotion, solution, cream, ointment, or dusting powder) , or epi-or transdermally (e.g., by use of a skin patch) .
  • a composition is formulated for topical administration, either as a single agent, or in combination with other therapeutic and/or prophylactic agents, as described herein or known in the art.
  • compositions are formulated for topical administration, e.g., to an area of non-intact skin.
  • Non-intact skin can include, but is not limited to, skin lesions, vesicles, chronic ulcers, cysts, blisters, bullae, open sores such as decubitus ulcers (bed sores) and other pressure sores, cellulitis sores, erysipelas lesions, wounds, burn wounds, or other conditions where the skin is damaged, broken, cracked, breached and/or otherwise compromised.
  • Topical formulations generally include a sterile buffer, such as a sterile PBS, water, or saline buffer, or a sterile SM buffer.
  • SM buffer suitable for use in certain embodiments of the instant invention comprises Tris-HCl, NaCl, and/or MgSO 4 7H 2 O, e.g., about 0.05 M Tris-HCl (pH 7.4-7.5) , about 0.1 M NaCl, and/or about 10 mM MgSO 4 H 2 O.
  • the formulation further comprises an SM buffer and 10 mM MgCl 2.
  • the formulation further comprises an SM buffer and about 20%to about 30%ethanol.
  • compositions may be combined with one or a combination of carriers for topical formulations, which can include, but are not limited to, an aqueous liquid, an alcohol base liquid, a water soluble gel, a lotion, an ointment, a nonaqueous liquid base, a mineral oil base, a blend of mineral oil and petrolatum, lanolin, liposomes, proteins carriers such as serum albumin or gelatin, powdered cellulose carmel, and combinations thereof.
  • carriers for topical formulations can include, but are not limited to, an aqueous liquid, an alcohol base liquid, a water soluble gel, a lotion, an ointment, a nonaqueous liquid base, a mineral oil base, a blend of mineral oil and petrolatum, lanolin, liposomes, proteins carriers such as serum albumin or gelatin, powdered cellulose carmel, and combinations thereof.
  • topical pharmaceutical compositions of the invention are provided in a hermetically sealed container.
  • the container may a vial, tube, bottle, ampoule, or the like; and may comprise or consist of glass, plastic, or other suitable material.
  • Ampoules for example, generally are produced industrially from short lengths of glass tubing, shaped by heating with gas torches and gravity. Computer vision techniques often are employed, e.g., for quality control.
  • the filling and sealing of ampoules may be done by automated machinery.
  • Blank ampoules can be purchased from scientific glass supply houses and sealed, e.g., with a small gas torch, preferably under inert atmospheres.
  • the container also may be filled with an inert gas, in addition to the pharmaceutical composition.
  • the composition is provided in an ampoule, or other suitable container, and transferred for use to a vehicle suitable for direct contact with non-intact skin, e.g., a patch, wipe, bandage, dressing, as described below.
  • the topical mode of delivery may include a smear, a spray, a bandage, a time-release patch, a liquid-absorbed wipe, and combinations thereof.
  • the composition is provided, either directly or in a carrier (s) , in a patch, wipe, bandage, dressing, or other vehicle suitable for direct contact with the skin, either intact or non-intact skin.
  • topical administration of a composition comprises use of a dressing.
  • the composition comprising a bacteriophage may be incorporated into a dressing and/or applied separately along with the use of a dressing.
  • a dressing promotes healing by keeping a wound moist, creating a barrier against infection, and/or keeping the surrounding skin dry.
  • Modes of administration described herein and/or known in the art may be used to deliver desired dosages of the bacteriophage and in accordance with suitable dosage regimens. Dosages and dosage regimens may vary depending on the particular formulation, route of administration, condition being treated, and other factors. Animal experiments can provide reliable guidance for the determination of effective doses in human therapy, e.g., as within the skill of the ordinary physician. Interspecies scaling of effective doses can be performed by one of ordinary skill in the art following the principles described, e.g., by Mordenti, J. et al. "The use of interspecies scaling in toxicokinetics" in Toxicokinetics and New Drug Development, Yacobi et al., Eds., Pergamon Press, New York 1989, pp 42-96.
  • the composition is a skin care product.
  • the skin care product is a paste, cream, lotion, gel, moisturizer, cleanser, or sunscreen. Examples of a skin care cosmetic dermatological compositions are referenced in U.S. Pub 2016/0235791A1.
  • Skin care products may be formulated by combining a present composition and a suitable cosmetic or pharmaceutical vehicle.
  • suitable cosmetic or pharmaceutical vehicle Non-limiting examples of components conventionally used in skin creams include thickeners; preservatives; lipid-soluble components; methoxycinnamate esters of medium-chain alcohols; benzophenone-3; fragrance; complexes of polyacrylamide, C 13 -C 14 isoparaffin, laureth-7, and water; and colorings.
  • concentrations of individual components present may vary widely, but often range from about 0.01%to about 10%, more usually from about 0.05%to about 5% (w/w) .
  • Non-limiting examples of thickeners xanthan gum, carrageenan, and combinations thereof.
  • Non-limiting examples of preservatives include methylparaben; butylparaben; propylparaben; a complex of propylene glycol, phenoxyethanol, chlorphenesin, and methylparaben; and combinations thereof. Suitable preservatives are commercially available.
  • the skin care product may also include lipid-soluble component (s) that provide smoothness.
  • lipid-soluble components include steareth-2; steareth-21; dimethicone; and branched-chain neopentanoate ester selected from the group consisting of octyldodecyl neopentanoate, heptyldodecyl neopentanoate, nonyldodecyl neopentanoate, octylundecyl neopentanoate, heptylundecyl neopentanoate, nonylundecyl neopentanoate, octyltridecyl neopentanoate, heptyltridecyl neopentanoate, and nonyltridecyl neopentanoate.
  • Steareth-2 is polyoxyethylene stearylether with 0.01%butylated hydroxyanisole and 0.005%citric acid added as preservatives.
  • Steareth-21 is polyoxyethylene stearylether with 0.01%butylated hydroxyanisole and 0.005%citric acid added as preservatives.
  • composition also may include a variety of other components such as coloring agents, fragrance, and the like.
  • the pH of the formulation may be adjusted with acceptable acids, bases or buffers.
  • the compositions also may contain diluents commonly used in the art such as water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol, dimethylsulfoxide (DMSO) dimethylformamide, oils, glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • diluents commonly used in the art such as water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate,
  • the skin care product is provided in a liquid or semisolid form (e.g., liquid, paste, gel, cream, lotion, etc. ) for topical application.
  • the skin care product may be applied topically to reduce inflammation, redness, or irritation.
  • the skin care product may be topically administered to treat an autoimmune and/or dermatological condition such as acne, psoriasis, and/or rosacea.
  • a subject treated for psoriasis, skin lesion or infection in accordance with the methods provided herein is a human who has or is diagnosed with a disorder.
  • a subject treated for the disorder in accordance with the methods provided herein is a human predisposed or susceptible to the disorder.
  • a subject treated for the disorder in accordance with the methods provided herein is a human at risk of developing the disorder.
  • a subject treated for psoriasis, skin lesion or infection in accordance with the methods provided herein is a human infant.
  • the subject is a human toddler.
  • a subject treated for infection in accordance with the methods provided herein is a human child.
  • a subject is a human adult.
  • a subject is a middle-aged human.
  • a subject is an elderly human.
  • a subject treated for the disorder in accordance with the methods provided herein is a human that is about 1 to about 5 years old, about 5 to 10 years old, about 10 to about 18 years old, about 18 to about 30 years old, about 25 to about 35 years old, about 35 to about 45 years old, about 40 to about 55 years old, about 50 to about 65 years old, about 60 to about 75 years old, about 70 to about 85 years old, about 80 to about 90 years old, about 90 to about 95 years old or about 95 to about 100 years old, or any age in between.
  • a subject treated for the disorder in accordance with the methods provided herein is a human that is 18 years old or older.
  • a subject treated for the disorder in accordance with the methods provided herein is a human child that is between the age of 1 year old to 18 years old. In a certain embodiment, a subject treated for the disorder in accordance with the methods provided herein is a human that is between the age of 12 years old and 18 years old. In a certain embodiment, the subject is a male human. In another embodiment, the subject is a female human. In one embodiment, the subject is a female human that is not pregnant or is not breastfeeding. In one embodiment, the subject is a female that is pregnant or will/might become pregnant, or is breast feeding.
  • a subject treated for the disorder in accordance with the methods provided herein is administered a pharmaceutical composition thereof, or a combination therapy before any adverse effects or intolerance to therapies.
  • a subject treated for the disorder in accordance with the methods provided herein is a human that has established resistance to previous antibiotic therapies other than treatment with the presently disclosed therapy.
  • a subject treated for infection in accordance with the methods provided herein is a human already receiving one or more conventional antibacterial therapies. Among these patients are patients who have developed infections that are resistant to antibiotics and patients with recurring infections despite treatment with existing therapies.
  • kits for use in methods of treatment of psoriasis, skin lesion or a bacterial infection can include a bacteriophage or composition provided herein, a second agent or composition, and instructions providing information to a health care provider regarding usage for treating the disorder. Instructions may be provided in printed form or in the form of an electronic medium such as a floppy disc, CD, or DVD, or in the form of a website address where such instructions may be obtained.
  • a unit dose of a bacteriophage or composition provided herein, or a second agent or composition can include a dosage such that when administered to a subject, a therapeutically or prophylactically effective dose of the bacteriophage or composition can be maintained in the subject for at least 1 days.
  • a bacteriophage or composition can be included as a sterile aqueous pharmaceutical composition or dry powder (e.g., lyophilized) composition.
  • suitable packaging includes a solid matrix or material customarily used in a system and capable of holding within fixed limits a bacteriophage provided herein and/or a second agent suitable for administration to a subject. Such materials include glass and plastic (e.g., polyethylene, polypropylene, and polycarbonate) bottles, vials, paper, plastic, and plastic-foil laminated envelopes and the like. If e-beam sterilization techniques are employed, the packaging should have sufficiently low density to permit sterilization of the contents.
  • kits described herein contain one or more containers, which contain bacteriophage as described.
  • the kits also contain instructions for mixing, diluting, and/or administrating the compounds.
  • the kits also include other containers with one or more solvents, surfactants, preservative and/or diluents (e.g., normal saline (0.9%NaCl) , or 5%dextrose) as well as containers for mixing, diluting or administering the components to the sample or to the patient in need of such treatment.
  • compositions of the kit may be provided as any suitable form, for example, as liquid solutions or as dried powders.
  • the powder When the composition provided is a dry powder, the powder may be reconstituted by the addition of a suitable solvent, which may also be provided.
  • a suitable solvent which may also be provided.
  • the liquid form may be concentrated or ready to use.
  • suitable solvents for drug compositions are well known and are available in the literature. The solvent will depend on the agent and the mode of use or administration.
  • kits comprise a carrier being compartmentalized to receive in close confinement one or more container such as vials, tubes, and the like, each of the container comprising one of the separate elements to be used in the method.
  • a carrier being compartmentalized to receive in close confinement one or more container such as vials, tubes, and the like, each of the container comprising one of the separate elements to be used in the method.
  • one of the container may comprise a positive control in an assay.
  • the kit may include containers for other components, for example, buffers useful in the assay.
  • the subject receiving a pharmaceutical composition of the invention is a mammal (e.g., bovine, ovine, caprine, equid, primate (e.g., human) , rodent, lagomorph or avian (e.g., chicken, duck, goose) ) .
  • the subject receiving a pharmaceutical composition of the invention is a human, and particularly a patient that suffers from or is at risk of suffering from psoriasis, skin lesion and bacterial infection.
  • treatment refers to obtaining a therapeutic benefit in a subject receiving the pharmaceutical composition.
  • the object is to eliminate, lessen, manage, decrease the severity of, prevent worsening, ameliorate, or slow the progression of the symptoms or underlying cause (e.g., bacterial infection) associated with the pathological condition or disorder.
  • the composition in certain embodiments, may act as a prophylactic or preventative measure, preventing the onset of psoriasis, skin lesion and infection caused by one or more bacteria.
  • prevention refers to obtaining a prophylactic benefit in a subject receiving the composition.
  • the object is to delay or prevent the symptoms or underlying cause (e.g., bacterial infection) associated with the pathological condition or disorder.
  • composition disclosed herein has activity against a plurality of bacterial strains.
  • the composition has activity against a plurality of strains of Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia or Rhodoferax.
  • another aspect of the invention provides methods of treating and/or preventing psoriasis, skin lesion and infections associated with a plurality of strains of Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia or Rhodoferax in both humans and animals using the composition.
  • the invention provides methods of treating and/or preventing infections associated with related species or strains of these bacteria.
  • the bacterial infection is an infection associated with psoriasis.
  • compositions of the invention comprises a therapeutically and/or prophylactically effective amount of one of more bacteriophage strains, as described herein.
  • a therapeutically and/or prophylactically effective amount refers to an amount required to bring about a therapeutic and/or prophylactic benefit, respectively, in a subject receiving said amount.
  • a therapeutically and/or prophylactically effective amount will depend on the particular formulation, route of administration, condition being treated, whether other agents or therapies are used in combination with methods of the invention, and other factors.
  • compositions disclosed herein are formulated as pharmaceutical compositions for use in treating and/or preventing bacterial infections associated with areas of non-intact skin.
  • the therapeutically and/or prophylactically effective amount will depend on the area of non-intact skin and the pharmaceutical compositions can be formulated to reflect same.
  • the pharmaceutical composition comprises phage strains where each is present in an amount corresponding to about 10 3 to about 10 13 phage particles/cm 2 of said area.
  • the therapeutic and/or prophylactic amount may correspond to at least about 10 4 , at least about 10 5 , at least about 10 6 , at least about 10 7 , at least about 10 8 , or at least about 10 9 , phage particles/cm 2 of the area of non-intact skin to be treated.
  • the therapeutic and/or prophylactic amount may correspond to less than about 10 13 , less than about 10 12 , less than about 10 11 , less than about 10 10 , less than about 10 9 , or less than about 10 8 phage particles/cm 2 of the area of non-intact skin to be treated.
  • each phage strain is present in the pharmaceutical composition in an amount corresponding to 10 7 to 10 9 phage particles/cm 2 of the non-intact skin area.
  • administration of a therapeutically effective amount of a composition results in improved clearing of psoriasis, such as a reduction in the area of psoriatic skin compared to the area before initiation of treatment.
  • the area can be expressed as a percentage of the initial psoriatic skin area, at one or more time points after initiation of treatment.
  • psoriatic skin area decreases by about 20-30%, about 30-40%, about 40-50%, about 50-60%, about 60-70%, about 70-80%, about 80-90%; or about 90-99%over a course of treatment with a composition of the invention.
  • the decrease in psoriatic skin area occurs at least by day 1 after treatment initiation (t1) , day 2 after treatment initiation (t2) , day 3 after treatment initiation (t3) , day 4 after treatment initiation (t4) , day 5 after treatment initiation (t5) , day 6 after treatment initiation (t6) , day 7 after treatment initiation (t7) , day 8 after treatment initiation (t8) , day 9 after treatment initiation (t9) , day 10 after treatment initiation (t10) , day 12 after treatment initiation (t12) , day 15 after treatment initiation (t15) , day 20 after treatment initiation (t20) , day 25 after treatment initiation (t25) , or day 30 after treatment initiation (t30) .
  • the psoriatic skin area is reduced by about 30%to about 40%, by at least day 9 after treatment initiation (t9) .
  • psoriatic skin area is reduced by about 40%to about 50%, by at least day 9 after treatment initiation (t9) .
  • wound area is reduced by about 50%to about 60%, by at least day 9 after treatment initiation (t9) .
  • psoriatic skin area is reduced by about 60%to about 70%, by at least day 9 after treatment initiation (t9) .
  • a composition disclosed herein is used as a single agent for treating or preventing infections caused by Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof.
  • the disclosed composition is used in further combination with other agents, including other bacteriophage (for example, that target a different species or strain of bacteria) , or with antibiotics that target the same or different kinds of bacteria, including bacteria selected from any gram-positive bacteria, any gram-negative bacteria, and any other groups of bacteria that is not classified as gram-positive or gram-negative.
  • the compositions of the invention may also be used in combination with any other means of treating psoriasis, skin lesion or infection known to one of skill in the art.
  • the composition according to the invention is used in combination with at least one phage strain against the same or a different bacteria species.
  • the composition is used in combination with at least one phage strain selected from the group consisting of bacteriophage strain Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof.
  • the composition is applied topically to reduce inflammation, redness, and/or irritation, and/or to improve the appearance of the skin.
  • the composition is applied topically to treat an autoimmune and/or dermatological condition such as acne, psoriasis, and/or rosacea.
  • a method of treating or preventing psoriasis, skin lesion and bacterial infection by administering a composition comprising one or more strains of bacteriophage provided herein.
  • the method comprises contacting a subject with a composition comprising one or more species of bacteriophage as described herein in an amount effective to prevent or treat the subject.
  • Provided herein are methods for treating or preventing psoriasis, skin lesion, or a bacterial infection in a subject by administering to a subject in need thereof the composition described herein in an amount effective to reduce the symptoms.
  • the disclosure also relates to a method of predicting or determining the efficacy of a bacteriophage therapy in a subject, wherein the method comprises a step of determining a lytic activity of one or more strains of bacteriophage Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof from a sample from said subject, such a lytic activity being indicative of an efficient treatment.
  • the method further optionally comprises a step of treating said subject by one or more bacteriophages having a lytic activity to one or more strains of bacteriophage Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof from a sample of said subject.
  • the invention provides a method for selecting a subject or determining whether a subject is susceptible to benefit from a bacteriophage therapy, wherein the method comprises the step of determining a lytic activity of one or more bacteriophages strains of Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination thereof from a sample of said subject, a lytic activity of one or more bacteriophages to at least one strain indicating a responder subject.
  • Another object of the invention relates to a method for predicting the response of a subject to a bacteriophage therapy, wherein the method comprises the step of determining a lytic activity of one or more bacteriophage strain Acinetobacter, Salmonella, Bacillus, Pseudomonas, Haemophilus, Enterobacteria, Bordetella, Mycobacterium, Burkholderia, Rhodoferax or a combination from a sample of said subject, a lytic activity of one or more bacteriophage of the invention to at least one strain being indicative of a good response to said therapy.
  • Eligible participants with psoriasis were recruited from the FAMILY cohort, a population-representative sample by Leung et aland the Hong Kong psoriasis patients association (Leung et al 2017) .
  • Family controls living in the same household as the participants with psoriasis were recruited.
  • Lesional skin samples from four different locations elbow, forearm, knee, and scalp
  • Healthy skin samples were collected at contralateral non-lesional sites as well as matching skin sites from family controls.
  • Eligible participants with psoriasis were experienced lesion at least one location of elbow, forearm, knee or scalp, and were non-lesional at the contralateral site of the lesional location.
  • Eligible family controls were subjects without psoriasis.
  • Swabs were collected from all eligible participants with psoriasis and family controls using eSwab (Copan Diagnostics) . Each skin sample was taken by swabbing back and forth for 25 times. The swabbing area was approximately equal to the area of a circle with a radius of 2 centimeters.
  • Genomic DNA was extracted from each sample using QIAamp DNA Mini isolation kit (QIAGEN) following the manufacturer’s instructions for swabs.
  • DNA library was prepared by KAPA Hyper Prep kit. Shotgun sequencing was performed using Illumina HiSeq 1500 platform (2 x 100 bp paired-end sequencing) .
  • Adapters and human contaminate were removed from raw sequences. IDBA-UD was used to de novo assemble reads after the quality control into contigs.
  • Bacterial OTU was classified through DIAMOND and MEGAN. Relative abundance (%) of normalized OTU reads count from MEGAN was used as the estimation of bacterial abundance.
  • Phage OTU was mined through VirSorter from assembled contigs of all samples. Non-redundant contigs with length ⁇ 10kb or circular (Roux et al 2017) and ranked as category one ( “most confident” predictions) by VirSorter were defined as phage OTU.
  • Phage taxonomy was annotated by BLAST (e value cut-off 10 -5 , hit with the highest bit-score) search against NCBI viral RefSeq database (v69, 2015-02) . Phage abundance was estimated by reads count mapped to phage contigs and normalized by the length of contig and total reads of each sample. Reads mapped to contigs annotated as Enterobacteria phage phiX174 sensu lato, a contamination of Illumina data inde novo assembly were removed (Mukherjee et al 2015) .
  • linear mixed effect model was used to test the difference of microbial diversity measures and relative abundance between psoriasis lesional skin vs healthy skin (contralateral non-lesional skin and family controls) .
  • the groups of samples (lesional skin vs healthy skin) was the fixed effect in the model, and the household was the random effect with the indication of samples from which subject (patient vs family control) in the same household as the random slope.
  • Sex was added as another fixed effect in the linear mixed model to test the effect of sex to skin microbiome composition. Linear mixed effect modeling was performed and p-values of fixed effects were estimated.
  • BMI body mass index
  • SAPASI self-administered psoriasis area and severity index.
  • Bacterial species microbiome composition was also not significantly influenced by the sex effect (FIG. 8B and FIG. 9B) .
  • FIG. 1 shows the distribution of top ten most abundant species ranked by the median abundance among all skin samples in phage community composition and bacterial community composition (order of top abundant phage species and bacterial species are listed in the FIG. 5 and FIG. 6) .
  • Salmonella phage vB_SenS-Ent2, Rhodoferax phage P26218, and Mycobacterium phage DrDrey were the three most abundant phage species in all samples.
  • Pseudomonas species, Acinetobacter species, and Cutibacterium acnes were the three most abundant bacterial species in all samples.
  • the bacterial species alpha diversity (FIG. 10B) was significantly higher (linear mixed effect model, P ⁇ . 001) for healthy skin (median [Q1-Q3] 3.65 [3.22-4.03] ) than lesional skin (median [Q1-Q3] 2.85 [2.41-3.03] ) .
  • FIG. 11A for phage
  • FIG. 11B for bacteria
  • Figure 2 displays the comparison of the microbiome beta diversity as measured by the primary principal coordinate (PCoA1) between lesional skin and healthy skin.
  • PCoA1 of phage species beta diversity ( Figure 2A) was significantly different (linear mixed effect model, P ⁇ .
  • Figure 3 shows the difference of the abundance for the top ten most abundant phage species between lesional skin and healthy skin.
  • the top ten most abundant phage species were significantly more abundant in healthy skin than lesional skin (linear mixed effect model, FDR adjusted P ⁇ . 05) .
  • Acinetobacter phage Presley, Salmonella phage vB_SenS-Ent2, and Bacillus phage SP-10 were the top three.
  • We further tested whether the abundance difference is ordinal with lesional skin as the lowest, contralateral non-lesional skin as intermediate and family controls skin as the highest. The ordered differences were significant for these phages (Jonckheere-Terpstra test, FDR adjusted P ⁇ . 05) . Details of the results of testing on the differentially abundant phage species were provided in FIG. 7.
  • the ten most abundant phage species with abundant host bacteria genus were further investigated to understand the phage-host relationship.
  • Pseudomonas phage O4 with host bacteria Pseudomonas were selected.
  • two phage species showed the capability to suppress the abundance of their host bacteria at the high level of abundance (definition of high vs low level of phage abundance described in Methods) .
  • the cutoffs for high vs low for Acinetobacter phage Presley and Pseudomonas phage O4 was 3.35 and 1.74, respectively.
  • the abundance of host bacteria Pseudomonas was significantly lower (two-sample t-test, P ⁇ . 001) in subjects with high level of Pseudomonas phage O4, comparing with subjects with low level of Pseudomonas phage O4.
  • Acinetobacter phage Presley and Pseudomonas phage O4 Two significantly differential abundant phage species between psoriasis lesional skin and healthy skin, Acinetobacter phage Presley and Pseudomonas phage O4, also demonstrate the capability in suppressing the abundance of their host Acinetobacter and Pseudomonas. Our novel finding informs us of the strong ties between the skin phage community and the skin bacterial community in psoriasis.
  • Acinetobacter phage Presley and Pseudomonas phage O4 could significantly limit the abundance of their host bacterial genera Acinetobacter and Pseudomonas, respectively.
  • Acinetobacter baumannii and Pseudomonas aeruginosa in the bacterial genera are known as notorious opportunistic pathogens that may induce skin infection (Guerrero et al 2010, Wu et al 2011) . Facing the multidrug-resistance of Acinetobacter baumannii and Pseudomonas aeruginosa, researchers have begun to examine the potential of phage therapy for infections.
  • Regeimbal et al found a cocktail of five phages (phages AB-Army1 and AB-Navy1–4) could promote the healing of wound infection caused by Acinetobacter baumannii in mice model (Regeimbal et al 2016) .
  • a single phage species phage PA709 was reported to be able to monitor the Pseudomonas aeruginosa induced wound infection (Vieira 2012) .
  • Two phage species in our study, Acinetobacter phage Presley and Pseudomonas phage O4, could be considered as part of a cocktail of phage-based therapeutic probiotic to facilitate the recovery process to healthy skin from lesional state.
  • Roux S Emerson JB, Eloe-Fadrosh EA, Sullivan MB. Benchmarking viromics: an in silico evaluation of metagenome-enabled estimates of viral community composition and diversity. PeerJ. 2017 Sep 21; 5: e3817.

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Abstract

L'invention concerne une thérapie phagique pour le traitement et la lutte contre le psoriasis, les lésions cutanées et les infections. Plus particulièrement, l'invention concerne une composition comprenant un bactériophage et ses utilisations. L'invention concerne un procédé de fabrication de la composition comprenant une ou plusieurs souches de bactériophage. L'invention concerne également une méthode de traitement et de prévention d'une maladie cutanéé tel que le psoriasis ou les lésions cutanées. L'invention concerne également une composition dermatologique comprenant une ou plusieurs souches de bactériophage et son utilisation cosmétique.
PCT/CN2019/110309 2018-10-11 2019-10-10 Compositions contenant des bactériophages et leurs utilisations pour le traitement ou la prévention des lésions cutanées et du psoriasis WO2020073946A1 (fr)

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CN116555192A (zh) * 2023-06-08 2023-08-08 合肥工业大学 一种裂解唐菖蒲伯克霍尔德氏菌的噬菌体及其应用
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CN112831475A (zh) * 2021-01-20 2021-05-25 南京农业大学 一株裂解性噬菌体及其在防控烟草土传青枯病的应用
CN112831475B (zh) * 2021-01-20 2022-07-08 南京农业大学 一株裂解性噬菌体及其在防控烟草土传青枯病的应用
WO2023237594A1 (fr) * 2022-06-07 2023-12-14 Rinocloud Limited Analyses de séquençage de microbiome améliorées
CN116555192A (zh) * 2023-06-08 2023-08-08 合肥工业大学 一种裂解唐菖蒲伯克霍尔德氏菌的噬菌体及其应用
CN116555192B (zh) * 2023-06-08 2024-01-30 合肥工业大学 一种裂解唐菖蒲伯克霍尔德氏菌的噬菌体及其应用

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