WO2020045580A1 - Microorganism adsorbent and method for sterilizing microorganisms using same - Google Patents

Abstract

Provided are a microorganism adsorbent capable of adsorbing and sterilizing microorganisms such as bacteria and viruses, said microorganism adsorbent being less expensive and eco-friendly, and a method for sterilizing microorganisms using the same. As the microorganism adsorbent, a volcanic ash soil containing 30 wt% or more of Al₂O₃ is used.

Description

Microbial adsorbent and microorganism sterilization method using the same

The present invention relates to a microorganism adsorbent for adsorbing microorganisms such as bacteria such as Escherichia coli and viruses such as avian influenza virus contained in manure and the like, and a method for sterilizing microorganisms using the same.

種 々 Various measures are taken against bacteria and viruses that are harmful to humans and livestock. For example, the method of treating livestock manure varies depending on the shape and animal species, but the processing of "open pile" and "undigging" is a source of water pollution and offensive odor, and needs improvement. . In the case where treated water generated by livestock farmers is discharged to public water bodies such as rivers and lakes, drainage standards (living environment items and health items) are applied based on the “Water Pollution Control Law”. As described above, although the drainage standard is set for the discharge of the treated water, it is needless to say that the number of coliform bacteria contained in the treated water is desirably set to zero as much as possible.

Here, chlorine or ozone injection, ultraviolet irradiation or the like is generally used as a method for removing coliform bacteria contained in the treated water, but chlorine injection has a problem that it is inexpensive but corrodes equipment such as steel piping. is there. In addition, ozone injection and ultraviolet irradiation have a problem that the treatment cost is high, so that it is difficult to introduce equipment especially for small-scale livestock farmers.

鳥 Also, avian influenza virus may be changed to highly pathogenic avian influenza virus by mutation. When highly pathogenic avian influenza virus is carried in the intestinal tract of wild birds, feces containing the virus are dropped around the poultry farm, and it is thought that they are a source of infection for chickens. Here, in order to prevent highly pathogenic avian influenza virus from entering the poultry house, it is important to spray lime or other chemicals around the poultry house. I was

The present inventors have found that a specific volcanic ash soil, particularly a specific Kikai Akahoya volcanic ash soil, not only has excellent adsorption performance for enterohemorrhagic Escherichia coli and avian influenza virus, but also has a surprisingly bactericidal effect.

The present invention has been made in view of such problems, and is intended to provide a microorganism adsorbent capable of adsorbing and sterilizing microorganisms such as bacteria and viruses at a low cost and a low burden on the environment, and a microorganism sterilizing method using the same. The purpose is to provide.

In order to solve the above problems, the microorganism adsorbent of the present invention,
It is characterized by using a volcanic ash soil containing 30 wt% or more of Al ₂ O ₃ .
According to this feature, water containing microorganisms such as bacteria and viruses is easily adsorbed, and the adsorption and sterilization ability is high.

The volcanic ash soil is characterized by being Kikai Akahoya volcanic ash soil containing 3 to 8 wt% of Fe ₂ O ₃ .
According to this feature, the ratio of Al ₂ O ₃ is three times or more as large as that of Fe ₂ O ₃ and it is easy to form a complex with aluminum ions and phosphate ions. Excellent.

The volcanic ash soil is characterized by comprising 39 to 65 wt% of SiO ₂ , 31 to 45 wt% of Al ₂ O ₃ , 3 to 8 wt% of Fe ₂ O _{3 and} 0 to 22 wt% of others.
According to this feature, there is little processing to natural Kikai Akahoya volcanic ash soil.

The volcanic ash soil is characterized by being fired.
According to this feature, it is possible to eliminate various germs contained in the natural Kikai Akahoya volcanic ash soil.

The calcination is performed at a temperature of 1000 ° C. or less.
According to this feature, those having small pores in the volcanic ash soil are likely to exist, and the microorganisms are easily adsorbed.

The volcanic ash soil is characterized by having at least pores having a diameter of 100 nm or less.
According to this feature, pores having a small diameter are likely to exist, and microorganisms are easily adsorbed.

The volcanic ash soil has a specific surface area of 3.9 m ² g ⁻¹ or more.
According to this feature, the surface area is large and the microorganisms are easily adsorbed.

The volcanic ash soil is a powder having a particle size of 1 mm or less.
According to this feature, since it is in the form of a powder, it is easy to spray and use it.

The volcanic ash soil is a powder having a soil particle density of 2.6 to 2.7 g / cm ³ .
According to this feature, since the soil particle density is low, it can be used in a state of being floated on water by stirring or aeration.

In order to solve the above-mentioned problems, a method for sterilizing microorganisms using a microorganism adsorbent of the present invention is characterized by using the microorganism adsorbent.
According to this feature, microorganisms such as bacteria and viruses can be sterilized at low cost and with less burden on the environment.

It is a graph which shows the relationship between the sintering temperature of red sea squirt and the pore size distribution which constitute the microorganism adsorbent in the example of the present invention. It is a figure which shows the experimental result which investigated the adsorption ability with respect to Escherichia coli of the red sea squirt which comprises the microorganism adsorption material in an Example. It is a figure showing the experimental result which investigated the bactericidal ability to Escherichia coli of the microorganism adsorbent in Example 1 of the present invention. It is a figure which shows the experimental result which investigated the adsorption capability with respect to avian influenza virus of the microorganism adsorbent in Example 2 of this invention. It is a graph which shows the result of the survival test of Escherichia coli adsorbed on the microorganism adsorbent in Example 5 of the present invention. 9 is a graph showing the results of a survival test of O-157 adsorbed on the microorganism adsorbent in Example 6 of the present invention.

The present inventors adsorb or sterilize bacteria such as enterohemorrhagic Escherichia coli (hereinafter sometimes simply referred to as “Escherichia coli”) contained in manure of livestock and microorganisms such as viruses such as avian influenza virus. It has been found that volcanic ash soils, especially natural Kikai Akahoya volcanic ash soils (hereinafter simply referred to as “Akahoya”) are excellent. As a result of the research, it was significant to pay attention to the components and the structure. First, this matter will be described.

The mechanism of adsorption or sterilization of microorganisms by the microorganism adsorbent will be described. The microbial adsorbent has innumerable pores with a diameter of 100 nm or less and has a large specific surface area, so it has excellent physical adsorption to microorganisms due to van der Waals force and hydrogen bonding, and also has excellent water absorption. ing. Furthermore, since the microorganism adsorbent has a high composition ratio of Al ₂ O ₃ (aluminum oxide) and Fe ₂ O ₃ (iron oxide) and shows hydrophilicity, after absorbing water, ionized Al ³⁺ (aluminum ion) and Fe ³⁺ (iron ion) shows high chemical bonding (chemical reactivity) with PO ₄ ^3- (phosphate ion) constituting the surface of the cell membrane of the microorganism (hydrophilic group of phospholipid), and insoluble AlPO ₄ (phosphate Aluminum) and FePO ₄ (iron phosphate) are produced. The chemical formula of the chemical bond between ionized Al ³⁺ and Fe ³⁺ and PO ₄ ^3- is shown in the following chemical formula 1.

The cell membrane of the microorganism constitutes a lipid bilayer by self-assembly of phospholipids, but PO ₄ ^3- constituting the surface of the cell membrane (hydrophilic groups of the phospholipid) is replaced with the microorganism adsorbents Al ³⁺ and Fe ^{3. The} formation of AlPO ₄ and FePO ₄ by chemical bonding with ³⁺ degrades the phospholipid. In particular, Escherichia coli is considered to be killed by the decomposition of the cell membrane due to the decomposition of the phospholipid. Escherichia coli suspended in the treated water without being adsorbed by the microorganism adsorbent is also killed by chemically bonding to Al ³⁺ and Fe ³⁺ dissolved in the treated water.

As described above, the microorganism adsorbent physically adsorbs the microorganism through the innumerable pores having a diameter of 100 nm or less, and has high chemical bonding between the ionized Al ³⁺ and Fe ³⁺ with PO ₄ ^3- constituting the cell membrane of the microorganism. By producing AlPO ₄ and FePO ₄ by the above, microorganisms can be efficiently adsorbed or sterilized.

形態 Embodiments for implementing the microorganism adsorbent according to the present invention will be described below based on examples. In this example, each test result such as the composition ratio of components is an average value of three or more samples.

The microbial adsorbent is obtained by crushing and sifting red sea squirts from the Higashi-Moryo-gun district of Miyazaki Prefecture, and then firing under the following conditions to kill soil-derived bacteria, stabilize constituent compounds, and maintain a certain shape and strength. To secure.
Temperature 800 ° C
Holding time 60 minutes (temperature rise 100 ° C / 1 hour)
Firing device (electric muffle furnace FUW220PA manufactured by ADVANTEC)

The component composition ratios of the red sea squirt before firing were as follows. The composition ratio of the components was analyzed according to JIS K0119: 2008 using an energy dispersive X-ray fluorescence analyzer EDX-720 manufactured by Shimadzu Corporation.
51.0 wt% SiO ₂
39.7 wt% Al ₂ O ₃
4.71 wt% Fe ₂ O ₃
1.49 wt% K ₂ O
1.40wt% CaO
0.51wt% MgO
0.53wt% TiO ₂
0.66wt% other

成分 The composition ratio of the components of the red sea squirt after firing was as shown in Table 1 below.

The red squirt after firing has an Al ₂ O ₃ component composition ratio of 38.3 wt% and a Fe ₂ O ₃ component composition ratio of 3.96 wt%, and Al ₂ O ₃ and Fe ₂ O _{3 with} respect to SiO ₂ . Ratio is high.

細孔 Here, the pore diameter distribution and the specific surface area change depending on the firing temperature, which will be described below. In addition, the pore diameter distribution was analyzed by a mercury intrusion method (JIS R1655) using AutoPore V9620 manufactured by Micromeritics.

As shown in the graph of FIG. 1, the pores of red sea squirt are distributed with a diameter of 0.01 to 10 μm (100 to 10000 nm), and at a firing temperature of 800 to 1000 ° C., pores having a diameter of 100 nm or less are 1 volume. % And pore distribution, but at a firing temperature of 1100 ° C. and 1150 ° C., pores having a diameter of 100 nm or less are less than 1% by volume and the pore distribution is lost. That is, it was confirmed that the pores were closed and the pore size distribution changed as the firing temperature increased.

比 The specific surface area was determined by BET method (JIS Z8830) using FlowSorb3 2310 manufactured by Micromeritics.

As shown in Table 2, the specific surface area of the red sea squirt was 68.7 m ² g ⁻¹ without firing, but was 36.0 m ² g ⁻¹ at the firing temperature of 800 ° C. and 9.90 at the firing temperature of 900 ° C. ^{54m 2 g -1, 3.9m 2 g} -1 at a firing temperature 1000 ℃, 1.35m ² g ^-1 at a firing temperature 1100 ° C., is reduced with 0.79 m ² g ^-1 at a firing temperature 1150 ° C.. That is, as the firing temperature increases, the specific surface area decreases as the pores are closed.

In addition, as shown in the photograph of FIG. 2, when the passed solution after filtering the suspension of Escherichia coli on red sea squirt fired at a firing temperature of 800 ° C. was cultured, no viable bacteria were detected (see the middle part of FIG. 2). . Similar results were obtained by culturing the washing solution that had been washed by passing the buffer solution again through the red sea squirt after filtering the suspension of Escherichia coli (see the lower part of FIG. 2). That is, in the firing of red sea squirt, the firing temperature is 1000 at which the pores having a diameter of 100 nm or less are distributed and the specific surface area is 3.9 m ² g ⁻¹ or more (preferably 36.0 to 69.0 m ² g ⁻¹ ). By carrying out at a temperature of not more than ℃ (preferably not more than 800 ℃), it is possible to maintain the physical adsorption ability of microorganisms by the pores of red sea squirt. If the calcination temperature is less than 100 ° C., it is found that the calcination temperature is preferably 100 to 1000 ° C., and more preferably 180 to 800 ° C., since the bacteria derived from the soil may not be sufficiently killed. . Further, it is preferable that there is no organic matter in the volcanic ash soil. In this case, the firing temperature is desirably 800 ° C. or higher.

(4) Next, the Akadama clay from the Kanuma area of Tochigi prefecture that has been processed in the same manner as the red sea squirt described above will be described.

The component composition ratio of Akadama before firing was as follows.
46.5wt% SiO ₂
34.9 wt% Al ₂ O ₃
13.2 wt% Fe ₂ O ₃
1.37wt% K ₂ O
0.58wt% CaO
1.31wt% MgO
1.35 wt% TiO ₂
0.79wt% other

成分 The component composition ratio of the fired red clay was as shown in Table 3 below.

Akadama clay after firing has an Al ₂ O ₃ component composition ratio of 33.0 wt%, a Fe ₂ O ₃ component composition ratio of 12.5 wt%, and Al ₂ O ₃ and Fe ₂ O with respect to SiO ₂ . The ratio of ₃ is high. Also, the composition ratio of Al ₂ O ₃ is lower and the composition ratio of Fe ₂ O ₃ is higher than that of red sea squirt.

粘土 Next, the clay produced in Shintomi area, Miyazaki Prefecture, which has been processed in the same manner as the above-mentioned Akahoya and Akadama clay will be described.

The component composition ratio before firing was as follows.
65.2 wt% SiO ₂
23.0 wt% Al ₂ O ₃
5.18 wt% Fe ₂ O ₃
3.71 wt% K ₂ O
0.24wt% CaO
1.65wt% MgO
0.81wt% TiO ₂
0.21wt% other

成分 The composition ratio of the clay after firing was as shown in Table 4 below.

The fired clay has a component composition ratio of Al ₂ O ₃ of 22.7 wt% and a composition ratio of Fe ₂ O ₃ of 4.76 wt%, and Al ₂ O ₃ and Fe ₂ O _{3 with} respect to SiO ₂ . Ratio is low. Also, the composition ratio of Al ₂ O ₃ is lower than that of red sea squirt, and the composition ratio of Fe ₂ O ₃ is substantially the same.

In addition, regarding the shirasu produced in the Miyakonojo area of Miyazaki prefecture that has been subjected to the same processing as the above-described red sea squirt, Akadama clay, and clay, the shirasu after firing is characterized by Al ₂ O _{3 with} respect to SiO ₂ as a characteristic of a general component. And the ratio of Fe ₂ O ₃ is low. Further, the composition ratio of Al ₂ O ₃ and Fe ₂ O ₃ is lower than that of red sea squirt.

Similarly, with respect to the Kanuma soil produced in the Kanuma area of Tochigi Prefecture, the ratio of Al ₂ O ₃ and Fe ₂ O ₃ to SiO ₂ is low in the fired Kanuma soil as a characteristic of a general component. Further, the ratio of Al ₂ O ₃ and Fe ₂ O ₃ is lower than that of red sea squirt.

In other words, Akahoya has a higher component composition ratio of Al ₂ O ₃ that produces Al ³⁺ having a higher stability constant than Akadama clay, clay, Shirasu and Kanuma soil, and Fe ₂ O ₃ component compared to Akadama clay. Since the composition ratio is low, for example, a complex is easily formed by Al ³⁺ (aluminum ion) and PO ₄ ^3- (phosphate ion) in feces, and the sequential reaction between the complex and PO ₄ ^3- constituting the cell membrane of the microorganism Then, microorganisms can be adsorbed more efficiently.

In this way, the microbial adsorbent is composed of red sea squirt, which is an inexhaustible natural material in a relatively shallow place underground in southern Kyushu, while having a high adsorption capacity for microorganisms such as bacteria and viruses, The burden on the environment can be reduced at low cost. The red sea squirt used as the microorganism adsorbent is not limited to the one produced in the district of Higashi-Moryo-gun, Miyazaki Prefecture as long as it is produced in southern Kyushu.

The volcanic ash soil such as red sea squirt in each of the following examples uses the above-mentioned components.

A microorganism adsorbent according to Example 1 will be described. The microbial adsorbent of Example 1 is constituted by pulverizing and sterilizing by dry heat under the following conditions, and then packing 10 g of red squirt adjusted to a particle size of 0.5 mm or less into a cylindrical column having a diameter of 15 mm. .
Temperature 180 ° C
Time 40 minutes Dry heat sterilizer (STA620DA manufactured by ADVANTEC)

After 10 ml of Escherichia coli (10 ⁵ cfu / ml) was passed through the microorganism adsorbent, 10 mg of red sea squirt in the column was stored at 4 ° C., and the viability of the adsorbed Escherichia coli was confirmed by a culture method. As can be seen, the E. coli adsorbed on the red sea squirt died over time, and no viable bacteria were detected after 6 days (see the left petri dish in FIG. 3). In addition, as a control experiment, when the same column was filled with 10 g of shirasu from Miyakonojo, Miyazaki Prefecture, and a similar experiment was performed, viable bacteria were detected until 17 days later (see the right petri dish in FIG. 3). That is, the microorganism adsorbent made of red sea squirt has the ability to adsorb and sterilize Escherichia coli.

In this example, the example using the red sea squirt sterilized by dry heat at 180 ° C. for 40 minutes was described, but the same result was obtained with the red sea squirt fired at the firing temperature of 800 ° C.

微生物 A description will be given of the microorganism adsorbent according to the second embodiment. The microorganism adsorbent of Example 2 is constituted by putting 0.2 g of red sea squirt which has been pulverized and sterilized by dry heat into a 0.6 ml column (microtube).

The microbial adsorbent is inoculated with 0.4 ml of avian influenza virus (H3N2 subtype) (HA value: 16 times, see the second row from the top in FIG. 4), and the passing solution after passing through the red sea squirt is diluted by 2- to 256 times. The agglutination titer (HA titer) was measured and, as shown in the photograph of FIG. 4, the avian influenza virus was adsorbed on the red sea squirt and did not agglutinate with the blood cells (HA titer negative, two columns from the bottom of FIG. 4). Eyes). As a control experiment, when 0.2 g of shirasu produced in the Miyakonojo district of Miyazaki was placed in a 0.6 ml column and the same experiment was performed, the bird influenza virus was hardly adsorbed to the shirasu and aggregated with blood cells ( HA number 8 times, see the bottom of FIG. 4). That is, the microorganism adsorbent made of red sea squirt has the ability to adsorb avian influenza virus.

微生物 A description will be given of the microorganism adsorbent according to the third embodiment. The microorganism adsorbent of Example 3 is a natural or calcined red sea squirt. The microorganism adsorbent is put into a large container together with the object to be treated, such as livestock manure, and then stirred to produce microorganisms such as Escherichia coli contained in the object to be treated. Adsorb or sterilize while promoting contact with The sludge (microbial adsorbent after use) generated in these treatments can be reduced to the ground, so that the treatment cost is low and sludge treatment equipment is not required.

微生物 A description will be given of the microorganism adsorbent according to the fourth embodiment. The microorganism adsorbent of Example 4 is a natural or baked red sea squirt processed into a powder having a particle size of 0.5 mm or less, and is easily sprayed. The powdery microorganism adsorbent is sprayed, for example, around the livestock barn, thereby adsorbing or sterilizing microorganisms existing around the livestock barn and preventing the invasion of microorganisms into the livestock barn. Also, by using red sea squirt, which is a natural material, as a microorganism adsorbent, even if it is sprayed in large quantities, the cost is low and the environmental load is small.

From these facts, the particle size is 1 mm or less, preferably 0.5 mm or less, more preferably 0.1 mm or less.

Furthermore, a red sea squirt as a material of the microorganism adsorbent may be stirred or aerated so that it can be used in a state of being suspended in water. Also, for example, water can be applied to trays installed at the entrances of livestock barns, etc., and the microorganism adsorbing material can be sprayed on the water surface and floated on the water surface to ensure that the microorganism adsorbing material adheres to shoes, livestock legs, etc. This ensures that microorganisms present in shoes, livestock legs, etc. are adsorbed or sterilized, and that the microorganisms can be prevented from entering the livestock barn.

For these reasons, the soil particle density is preferably 2.6 to 2.7 g / cm ³ , and more preferably the density is as low as possible. The soil particle density was determined according to JIS A1202: 2009.

Example 5 describes a survival test of Escherichia coli. The microbial adsorbent of Example 5 was pulverized, sterilized by dry heat under the following conditions, and then adjusted to a particle size of 0.5 mm or less, 10 g each of Akahoya, Akadama, Kanuma, and Shirasu in a cylindrical column with a diameter of 15 mm. Respectively.
Temperature 180 ° C
Time 40 minutes Dry heat sterilizer (STA620DA manufactured by ADVANTEC)

After passing a certain amount of E. coli through the microbial adsorbent, 10 mg each of red sea squirt, Akadama soil, Kanuma soil and Shirasu in the column were stored at 4 ° C., and the viability of the adsorbed E. coli was confirmed by a culture method. As shown in the graph, it was confirmed that the number of Escherichia coli adsorbed on the red sea squirt, Akadama soil, Kanuma soil and Shirasu decreased over time. As described above, the microorganism adsorbent made of red sea squirt, Akadama soil, Kanuma soil and Shirasu has the ability to adsorb and sterilize Escherichia coli.

Here, since the number of bacteria is reduced to 1/100, that is, 99% of the bacteria are killed, it can be said that a significant bactericidal effect is obtained. The number of days when the number of bacteria is less than 1/100, that is, the number of days when E. coli is killed, is E. coli adsorbed on Akashiya and Akadama soil three days later, E. coli adsorbed on Kanuma soil nine days later, and E. coli adsorbed on Shirasu After 17 days, the bactericidal effect was confirmed. As described above, it was confirmed that the microorganism adsorbent made of red sea squirt and red slag can obtain a bactericidal effect in a short period of time. The number of Escherichia coli adsorbed on red sea squirt, Akadama soil and Kanuma soil has been reduced to about 1 log @ cfu / g after 17 days, and it has been confirmed that almost all bacteria are killed over a long period of time. Was done.

Example 6 describes a survival test of enterohemorrhagic Escherichia coli O-157. In addition, what was manufactured like Example 5 was used for the microorganism adsorbent.

After a certain amount of enterohemorrhagic Escherichia coli O-157 (hereinafter simply referred to as “O-157”) was passed through the microorganism adsorbent, 10 mg each of Akahoya, Akadama soil, Kanuma soil and Shirasu in the column was added to each of 4 mg. The viability of the adsorbed O-157 was confirmed by a culture method. The O-157 adsorbed on the red sea squirt, the Akadama soil, and the Kanuma soil was over time, as shown in the graph of FIG. It was confirmed that the number of bacteria decreased. As described above, the microorganism adsorbent made from red sea squirt, Akadama soil, and Kanuma soil has O-157 adsorption / sterilization ability.

Here, since the number of bacteria is reduced to 1/100, that is, 99% of the bacteria are killed, it can be said that a significant bactericidal effect is obtained. The number of days when the number of bacteria is less than 1/100, that is, the number of days when O-157 is killed, is O-157 adsorbed on Akahoya and Akadama soil after 7 days, and O-157 adsorbed on Kanuma soil is 14 days or more. Later, the bactericidal effect was confirmed respectively. As described above, it was confirmed that the microorganism adsorbent made of red sea squirt and red slag can obtain a bactericidal effect in a short period of time. The number of bacteria of O-157 adsorbed on red sea squirt and Akadama soil decreased to about 1 log @ cfu / g after one month, and it was confirmed that almost all bacteria were killed over a long period of time. Was.

As shown in Table 5, from Examples 5 and 6, as shown in Table 5, the microorganism adsorbents made of red sea squirt and Akadama soil showed a significant effect on Escherichia coli and O-157 as compared with the microorganism adsorbents made of Kanuma soil and Shirasu. It has excellent sterilization ability. In Table 5, “○” indicates that there is a significant sterilizing ability, “△” indicates that the sterilizing ability is required although a long period is required, and “×” indicates that the sterilizing ability is insufficient. Is shown.

Also, referring to FIG. 5, the bactericidal ability against Escherichia coli in a short period of time (within 3 days), that is, the rate of decrease in the number of bacteria from day 0 to day 3 is superior to Akahoya over Akadama clay. Similarly, referring to FIG. 6, the bactericidal ability of O-157 in a short period (within 7 days), that is, the rate of reduction of the number of bacteria from day 0 to day 7 is superior to Akahoya.

As described above, the embodiments of the present invention have been described with reference to the drawings. However, the specific configuration is not limited to these embodiments, and even if there are changes and additions without departing from the gist of the present invention, they are included in the present invention. It is.

For example, in the above-described Examples 1 to 4, an example was described in which red or green sea squirt was used as a microorganism adsorbent after processing such as natural or sintering, but the present invention is not limited to this, and Al ₂ O ₃ containing 30 wt% or more is contained. If it is a volcanic ash soil, for example, it may be natural akadama soil or calcined akadama soil, and processed so as to contain 30 wt% or more of Al ₂ O ₃ in a volcanic ash soil such as Kanuma soil, clay or shirasu. It may be processed.

In addition, the microorganism adsorbent has an adsorption effect on microorganisms other than Escherichia coli (eg, Staphylococcus aureus, Salmonella spp., Campylobacter, Bacillus subtilis spores, Bacillus anthracis spores), and viruses other than avian influenza virus. Play.

微生物 In addition, the microorganism adsorbent is not limited to firing, and may be sterilized by, for example, ultraviolet light.

Industrial applications

1. Use as an adsorbent for livestock raising environment purification systems.
2. Use as an air purification system, for example, as an adsorbent for adsorbing microorganisms in the atmosphere.
3. Water purification systems, for example eutrophic salt (phosphorus) removal in closed water bodies (lake) and use as adsorbent for microorganisms such as Escherichia coli or coliforms.
4. Use as an adsorbent for bacteria and viruses such as anthrax spores in bioterrorism control systems.
5. Use as feed containing pathogen-adsorbed substances.

Claims (10)

1. A microorganism adsorbent characterized by using a volcanic ash soil in which Al ₂ O ₃ is 30 wt% or more.
2. The microorganism adsorbent according to claim 1, wherein the volcanic ash soil is Kikai Akahoya volcanic ash soil containing 3 to 8 wt% of Fe ₂ O ₃ .
3. The volcanic ash soil according to claim 1, wherein SiO ₂ is 39 to 65 wt%, Al ₂ O ₃ is 31 to 45 wt%, Fe ₂ O ₃ is 3 to 8 wt%, and others are 0 to 22 wt%. 3. The microorganism adsorbent according to 2.
4. The microorganism adsorbent according to any one of claims 1 to 3, wherein the volcanic ash soil is fired.
5. (5) The microorganism adsorbing material according to any one of (1) to (4), wherein the calcination is performed at 1000 ° C. or lower.
6. The microorganism adsorbent according to any one of claims 1 to 5, wherein the volcanic ash soil has pores having a diameter of at least 100 nm or less.
7. 7. The microorganism adsorbent according to claim 1, wherein the volcanic ash soil has a specific surface area of 3.9 m ² g ⁻¹ or more.
8. The microorganism adsorbent according to any one of claims 1 to 7, wherein the volcanic ash soil is a powder having a particle size of 1 mm or less.
9. The microorganism adsorbent according to any one of claims 1 to 8, wherein the volcanic ash soil is a powder having a soil particle density of 2.6 to 2.7 g / cm ³ .
10. 微生物 A method for sterilizing microorganisms using the microorganism adsorbent according to any one of claims 1 to 9.

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