WO2020032429A1

WO2020032429A1 - Cassette for biopsy and biopsy method using same

Info

Publication number: WO2020032429A1
Application number: PCT/KR2019/009057
Authority: WO
Inventors: 황성욱
Original assignee: (의) 삼성의료재단
Priority date: 2018-08-08
Filing date: 2019-07-23
Publication date: 2020-02-13
Also published as: KR20200017276A; KR102151161B1

Abstract

According to one embodiment of the present invention, provided is a cassette, for biopsy, comprising: a body unit which has one side open and can contain a tissue specimen; a cover unit which enables opening and closing of the body unit and has one or more through hole portions; and a penetration unit which passes through the through hole portions and can be coupled to the cover unit.

Description

Cassette for biopsy and histological method using same

Embodiments of the present invention relate to a histological cassette and a method for histological examination using the same.

In general, histological examination is performed for the treatment and diagnosis of the disease, such as determining the malignancy of the tumor, determining the extent of the lesion, determining the success and prognosis of the resection. The most commonly used method to study tissue is to create a tissue sample that can be observed under an optical microscope.

When preparing a tissue sample, first identify the lesion of the tissue and cut the lesion area to the size of the cassette and insert the cassette into the tissue processor to fix, dehydrate, clear, and so on. You will go through the process of.

In the case of conventional tissue sample preparation, the paraffin is infiltrated and infiltrated when the process is performed. However, when the tissue is thick or not fixed, the dehydration, transparent, and paraffin infiltration processes are not performed smoothly. There was a problem in that the production time is delayed, or a defective tissue in which paraffin does not penetrate.

Background art of the present invention is disclosed in Republic of Korea Patent Publication No. 10-1500833 (2015.03.03 registered, the name of the invention: Embedding cassette for histological examination).

The present invention has been made to improve the above problems, to provide a tissue test cassette and a method for histological examination using the same that can improve the speed and accuracy of tissue preparation for tissue examination by infiltrating the reagent directly into the tissue sample. do.

According to an embodiment of the present invention, one side is open to the body portion that can contain a tissue sample; A cover part capable of opening and closing the main body part and having at least one through hole part formed therein; And a penetrating portion penetrating through the through hole and coupled to the cover portion.

In the present invention, the penetrating portion is formed with openings at both sides, it may be formed in a hollow (Tube) shape.

In the present invention, the penetrating portion may be formed to decrease in width in one direction.

In the present invention, the thickness of the penetrating portion may decrease in one direction.

In the present invention, the penetrating portion, the opening is formed on both sides, the penetrating body formed in a hollow tubular shape; And a base portion coupled to the penetrating body and fixing the penetrating body in position.

In the present invention, the penetrating portion may further include a flow path portion coupled to the base portion, wherein the penetrating body, the base portion and the flow path portion may be in communication.

In the present invention, the penetrating body may be formed to decrease in width in one direction.

According to an embodiment of the present invention, one side is open to contain a tissue sample, at least one through-hole body portion is formed; And a cover part capable of opening and closing the main body part and coupled to the main body part. And a penetrating portion penetrating through the through-hole portion and coupled to the main body portion.

According to an embodiment of the present invention, a disposing step of containing a tissue specimen in the body portion; A fixing step of passing a penetrating part through a through hole formed in a cover part coupled to the main body part and penetrating the tissue sample with the penetrating part to fix the position of the tissue sample; And a penetrating step of injecting a reagent into the penetrating unit and penetrating the reagent into the tissue sample.

According to an embodiment of the present invention, a disposing step of containing a tissue specimen in the body portion; A fixing step of passing a penetrating part through a passage hole formed in the main body part and penetrating the tissue sample with the penetrating part to fix the position of the tissue sample; And a penetrating step of injecting a reagent into the penetrating unit and penetrating the reagent into the tissue sample.

According to another embodiment of the present invention, a histological cassette may include: a main body part having an upper portion thereof open to hold a tissue sample; And a cover part covering the body part and having a plate shape in which at least one through hole part is formed, wherein the cover part includes a reagent penetrating tube surrounding the through hole part.

In the present invention, the reagent penetrating tube may be a hollow tube that becomes narrower in one direction.

In the present invention, the reagent inlet tube may be in the form of a cone (cone) with the top and bottom open.

In the present invention, the thickness of the reagent infiltrating tube may decrease in one direction.

In the present invention, the reagent penetrating tube may have a pointed end to penetrate the tissue specimen.

Cover portion of the histology cassette according to another embodiment of the present invention, the plate formed with one or more through-holes; And a reagent penetrating tube surrounding the passage hole and having a pointed end to penetrate the tissue sample.

In the present invention, the reagent penetration tube may be a hollow tube that becomes narrower in one direction.

Tissue examination method according to another embodiment of the present invention, the step of putting a tissue specimen in the body portion; Fixing the position of the tissue sample by penetrating the tissue sample with a reagent penetration tube formed in a cover part; And injecting a reagent into the reagent penetration tube to infiltrate the reagent into the tissue sample.

Other aspects, features, and advantages other than those described above will become apparent from the following drawings, claims, and detailed description of the invention.

The bioassay cassette and the histological examination method using the same according to the present invention have an effect of infiltrating a reagent directly into a tissue sample to improve the speed and accuracy of tissue preparation for biopsy compared to the conventional natural immersion method.

1 is a perspective view showing a histology cassette according to an embodiment of the present invention.

2 is an exploded perspective view illustrating a cover part and a penetration part according to an exemplary embodiment of the present invention.

3 is a perspective view showing a histology cassette according to an embodiment of the present invention.

FIG. 4 is a front sectional view showing the II ′ section of FIG. 3.

5 is an enlarged view illustrating an enlarged portion A of FIG. 4.

Figure 6 is a front cross-sectional view showing a histology cassette according to an embodiment of the present invention.

7 is a side view showing a biopsy cassette according to an embodiment of the present invention.

8 is an exploded perspective view illustrating a main body part and a penetrating part according to an embodiment of the present invention.

Figure 9 is a front sectional view showing a histology cassette according to an embodiment of the present invention.

10 is a flowchart illustrating a biopsy method according to an embodiment of the present invention.

11 and 12 are exploded and combined perspective view of the histology cassette according to an embodiment of the present invention, respectively.

FIG. 13 is a cross-sectional view taken along the line II-II ′ of the histological cassette of FIG. 12, FIG. 14 is an enlarged view of region B of FIG. 13, and FIG. 15 is a reagent penetrating reagent into a tissue sample using a reagent penetrating tube. It is sectional drawing which showed the method of making.

As the invention allows for various changes and numerous embodiments, particular embodiments will be illustrated in the drawings and described in detail in the written description. Effects and features of the present invention, and methods for achieving them will be apparent with reference to the embodiments described below in detail in conjunction with the drawings. However, the present invention is not limited to the embodiments disclosed below but may be implemented in various forms.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, and the same or corresponding components will be denoted by the same reference numerals, and redundant description thereof will be omitted. .

In the following embodiments, the terms first, second, etc. are used for the purpose of distinguishing one component from other components rather than a restrictive meaning.

In the following examples, the singular forms “a”, “an” and “the” include plural forms unless the context clearly indicates otherwise.

In the following examples, the terms including or having have meant that there is a feature or component described in the specification and does not preclude the possibility of adding one or more other features or components.

In the following embodiments, when a part such as a film, a region, a component, or the like is on or on another part, not only is it directly above the other part, but also another film, a region, a component, etc. is interposed therebetween. This includes any case.

In the drawings, components may be exaggerated or reduced in size for convenience of description. For example, the size and thickness of each component shown in the drawings are arbitrarily shown for convenience of description, and thus the present invention is not necessarily limited to the illustrated.

Where certain embodiments may be implemented differently, a particular process order may be performed differently from the described order. For example, two processes described in succession may be performed substantially concurrently, or may be performed in the reverse order.

In the following embodiments, when a film, a region, a component, or the like is connected, not only the film, the region, the components are directly connected, but also another film, the region, the components intervening in between the film, the region, the components And indirectly connected. For example, in the present specification, when a film, a region, a component, and the like are electrically connected, not only the film, the region, the component, etc. are directly electrically connected, but other films, regions, components, etc. are interposed therebetween. This includes indirect electrical connections.

1 is a perspective view showing a histology cassette according to an embodiment of the present invention. 2 is an exploded perspective view illustrating a cover part and a penetration part according to an exemplary embodiment of the present invention. 3 is a perspective view showing a histology cassette according to an embodiment of the present invention. FIG. 4 is a front sectional view showing the II ′ section of FIG. 3. 5 is an enlarged view illustrating an enlarged portion A of FIG. 4. Figure 6 is a front cross-sectional view showing a histology cassette according to an embodiment of the present invention. 7 is a side view showing a biopsy cassette according to an embodiment of the present invention. 8 is an exploded perspective view illustrating a main body part and a penetrating part according to an embodiment of the present invention. Figure 9 is a front sectional view showing a histology cassette according to an embodiment of the present invention.

1 to 9, the histological cassette 1 according to an embodiment of the present invention may include a main body part 100, a cover part 200, and a penetration part 300.

Referring to FIG. 1, the main body 100 according to an exemplary embodiment of the present invention may have one side (the upper reference side of FIG. 1) opened, and may be combined with the cover 200 to be described later. The main body 100 is a place containing a tissue sample TS therein, and may be formed in a box shape.

Body portion 100 may be formed of a material such as plastic, stainless (Stainless) so as not to be corroded by chemicals such as formalin used in the histological examination process.

1 and 8, at least one passage hole 101 may be formed on a bottom surface (see FIG. 1) of the main body part 100. The passage hole 101 may provide a space through which a paraffin used in the embedding process passes.

In unfixed raw materials or fixed materials, it is difficult to cut them thinly as it is, so that the tissue piece has a rigidity suitable for cutting, and a hard part or a soft part. The operation to make it equally convenient without cutting the difference between them is called embedding.

Referring to FIG. 1, an inclined surface may be formed on the front portion (see FIG. 1) of the main body 100 according to an embodiment of the present invention for labeling. Since the inclined surface is formed in the front portion of the main body 100, the user can easily recognize what the tissue sample (TS) stored in the histology cassette (1).

1 to 4, the cover part 200 according to the exemplary embodiment of the present invention may open and close the main body part 100, and at least one through hole part 210 may be formed. In the present invention, the through-hole part 210 is formed in a circular shape, but is not limited thereto, and various modifications may be made to correspond to the shape of the penetrating part 300 to be described later, specifically, the penetrating body 310. .

Cover portion 200 according to an embodiment of the present invention may cover the upper portion (see Fig. 1) of the main body portion 100 and is formed in a plate shape (platform), although not shown in the present invention, the main body portion 100 Of course, it can be formed to be rotatably coupled to.

Referring to FIG. 1, one side of the cover part 200 may include a locking part for coupling with the main body part 100, and a handle part for allowing the user to easily open and close the cover part 200 on the other side opposite thereto. . However, the present invention is not limited thereto.

At least one through hole 210 is formed in the cover part 200, and the penetrating part 300 passes through the through hole 210 and penetrates the tissue sample TS disposed inside the body part 100. It is possible to provide a route of penetration so that

The passage hole 210 formed in the cover part 200 may have various sizes and arrangements according to the characteristics of the tissue specimen TS embedded therein.

1 to 5, the penetrating part 300 according to an embodiment of the present invention penetrates the through hole part 210 formed in the cover part 200, and can be coupled to the cover part 200. .

In addition, referring to FIGS. 8 and 9, the penetrating part 300 penetrates the through hole part 101 formed in the main body part 100, and can be coupled to the main body part 100.

Openings are formed at both sides of the penetrating part 300, and the reagent L may penetrate into the tissue sample TS through a flow path formed in the penetrating part 300.

The penetrating part 300 according to the exemplary embodiment of the present invention may include a penetrating body 310, a base part 320, and a flow path part 330. The penetrating body 310 may have openings formed at both sides (upper and lower sides of FIG. 3), and may be formed in a hollow tube shape. As a result, a reagent (L) such as paraffin may flow through a flow path formed inside the penetrating body 310, and the reagent (L) may penetrate the tissue sample (TS).

4 to 6, the penetrating part 300, specifically, the penetrating body 310 may include a fixing agent such as formalin used in the fixing process or a reagent such as paraffin used in the embedding process. L) may provide a flow path to and from this place.

4 and 5, the penetrating part 300, in detail, the penetrating main body 310 according to an embodiment of the present invention may be formed as a separate body from the main body part 100 or the cover part 200. It is not limited to this, and various modifications are possible, such as being formed integrally.

Referring to FIGS. 1 and 4, the penetrating part 300, specifically, the penetrating main body 310 may be located at an upper side of the main body part 100 (based on FIG. 1) when the main body part 100 is coupled to the main body part 100. have.

When the penetration body 310 is coupled to the cover part 200, a predetermined section (lower side of FIG. 4) may be located below the cover unit 200 (refer to FIG. 4). As a result, a predetermined section of the penetrating body 310 is positioned inside the main body 100, and the reagent L is penetrated into the tissue sample TS accommodated in the main body 100. Can be contacted directly.

Referring to FIG. 5, the penetrating part 300 according to an exemplary embodiment of the present invention, specifically, the penetrating body 310 may be formed as a hollow tube that is narrower in one direction (based on FIG. 5). The lower side (see FIG. 5) of the penetrating body 310 may be formed in a cone shape in which a lower end portion (see FIG. 5) is opened.

In other words, the penetrating main body 310 may have a lower end. At this time, the width of the lower surface of the open penetration body 310 may be 0.1cm to 0.5cm, but is not limited thereto.

3 to 6, when the reagent L is introduced through the upper portion (see FIG. 6) of the penetrating body 310 according to an embodiment of the present invention, the reagent L is the penetrating body 310. It may flow through the body portion 100 through the.

In accordance with an embodiment of the present invention, the thickness of the penetrating part 300, in particular, the penetrating body 310 may decrease in one direction (down direction based on FIG. 5). Referring to FIG. 5, the thickness W _u2 of the wall Wall of the penetration body 310 disposed on the lower side is smaller than the thickness W _u1 of the wall of the penetration body 310 disposed on the upper side. Can be formed.

That is, the cross section of the penetrating body 310 may be formed to be sharper toward the lower side (see FIG. 5).

Penetration body 310 according to an embodiment of the present invention may have a pointed end to penetrate the tissue specimen (TS). Accordingly, the pointed end of the penetrating body 310 may penetrate the surface of the tissue sample TS as shown in FIG. 6.

The penetrating part 300, specifically, the penetrating body 310 may be formed of a material such as metal or stainless steel in order to maintain a sharp shape.

Since the penetrating part 300, specifically, the lower end of the penetrating main body 310 is sharply formed, the penetrating main body 310 can penetrate a tissue sample (TS) composed mainly of soft and soft fatty tissue such as breast tissue.

Therefore, the tissue sample (TS) can be stored in the biopsy cassette (1) in a punctured state, and the reagent (L) such as paraffin directly into the 'inside' of the adipose tissue sample (TS) through the penetrating body 310. May be injected.

In addition, there is an effect that the predetermined section of the penetration main body 310 can be positioned inside the main body 100 through the through hole 210. A predetermined section of the penetrating body 310 located inside the main body 100 may be formed at half the height of the main body 100, but is not limited thereto.

Referring to FIG. 7, the base part 320 according to an embodiment of the present invention is coupled to the penetrating main body 310, and may fix the penetrating main body 310. The base portion 320 is formed in a plate shape, and the plurality of penetration bodies 310 may be fixed in position.

6 and 9, the penetrating body 310 according to the exemplary embodiment of the present invention may have a protrusion 311 protruding outward. The outer diameter of the hooking portion 311 may be formed larger than the diameter of the passage hole 210 formed in the cover portion 200 or the passage hole 101 formed in the main body 100, thereby penetrating portion 300 ) May be prevented from penetrating the passage hole 210 when the penetrating main body 310 positioned above the cladding part 311 when the cover part 200 is coupled to the cover part 200.

Hanging portion 311 according to an embodiment of the present invention may be formed in a band shape along the outer peripheral surface of the penetrating body (310).

Due to the base portion 320, the plurality of penetration bodies 310 are formed to have the same height, and the reagent L can be injected at the same time into the tissue sample TS disposed inside the main body portion 100. It works.

Referring to FIG. 7, the flow path part 330 according to an embodiment of the present invention is coupled to the base part 320, and may be formed to communicate with the base part 320 and the penetration body 310. Therefore, the reagent L is supplied from the outside through the flow path part 330, and the reagent is transferred to the tissue sample TS through the base part 320 and the penetrating body 310 through the flow path formed in the flow path part 330. Let (L) be injected.

In the histology cassette 1 according to the embodiment of the present invention, the penetrating part 300 directly penetrates the reagent L into the tissue sample TS, thereby allowing the soft tissue specimen TS such as adipose tissue. Processes such as fixing, washing with water, dehydration, and transparent can be performed efficiently.

In addition, it is possible to create a state suitable for embedding and cutting (Microtome) has the effect of improving the efficiency of biopsy.

Hereinafter, a histological examination method using a histological cassette 1 according to an embodiment of the present invention will be described.

1, 7 and 10, the method for examining the tissue according to an embodiment of the present invention may include a placement step (S10), a fixing step (S20), a penetration step (S30).

In general, the tissue sample TS accommodated in the histological cassette 1 is exposed to a fixed solution, a dehydration agent, and a clearing agent in a tissue processor. However, adipose tissue, such as breast tissue, does not penetrate well into the tissue specimen (TS) due to its characteristics.

This makes the tissue specimen (TS), especially adipose tissue, remain lumped and hardened in the subsequent paraffin embedding process, and it becomes difficult to cut the tissue specimen (TS) into thin sections during the cutting process. In addition to increasing the likelihood of errors in the test, there is a problem that the risk of injury to the user when cutting thinly to put the adipose tissue in the histological cassette (1) for visual observation.

Referring to FIG. 10, in the disposition step S10, the tissue sample TS is disposed in the main body 100.

In the fixing step (S20) according to an embodiment of the present invention, the cover part 200 is coupled to the main body part 100, and the penetration part 300 is formed in the through-hole part 210 formed in the cover part 200, specifically, The penetrating body 310 is passed through, and the penetrating unit 300 may penetrate the tissue sample TS to fix the position of the tissue sample TS.

In addition, in the fixing step (S20), the penetrating part 300, specifically, the penetrating body 310 passes through the passage hole portion 101 formed in the main body portion 100, the tissue sample (TS) as the penetrating portion 300 The position of the tissue specimen (TS) can be fixed by penetrating.

In the penetrating step (S30) according to an embodiment of the present invention to inject the reagent (L) in the infiltration unit 300, and to infiltrate the reagent (L) into the tissue sample (TS), which is about 13 hours Compared to the natural immersion of the abnormal tissue specimen (TS) in paraffins there is an effect that the permeability can be improved.

In addition, since the penetrating body 310 is in direct contact with the tissue sample TS, the tissue sample TS may be fixed in the main body 100 to prevent the tissue sample TS from moving. It works.

Particular implementations described in the present invention are embodiments and do not limit the scope of the present invention in any way. For brevity of description, descriptions of conventional electronic configurations, control systems, software, and other functional aspects of the systems may be omitted. In addition, the connection or connection members of the lines between the components shown in the drawings are illustrative of the functional connection and / or physical or circuit connections as an example, in the actual device replaceable or additional various functional connections, physical It can be represented as a connection, or circuit connections. In addition, unless otherwise specified, such as "essential", "important" may not be a necessary component for the application of the present invention.

Therefore, the spirit of the present invention should not be limited to the above-described embodiments, and the scope of the spirit of the present invention is defined not only in the claims below, but also in the ranges equivalent to or equivalent to the claims. Will belong to.

Hereinafter, the configuration, operation principle and effects of the histological cassette 1000 according to another embodiment of the present invention will be described.

11 and 12 are exploded perspective and combined perspective views of the histological cassette 1000 according to another embodiment of the present invention, respectively.

The histology cassette 1000 according to another embodiment of the present invention includes a cover portion 1200 having a body portion 1100 and a reagent penetrating tube 1210.

Referring to FIG. 11, the main body 1100 may contain a tissue sample (TS, see FIG. 15) therein, and may have a box shape having an open top.

The body unit 1100 may include plastic, stainless, or the like that is not corroded to chemicals such as formalin used in the histological examination. One or more through hole parts 1100H may be formed on the bottom surface of the body part 1100. The passage hole part 1100H may provide a space through which a paraffin used in the embedding process passes. One side of the body portion 1100 may be inclined for labeling.

The cover part 1200 may cover an upper portion of the main body 1100. The cover part 1200 may include a flat plate. One side of the cover portion 1200, the engaging portion for coupling with the main body portion 1100, the other side may be disposed in the handle portion for the user to easily open and close the cover portion 1200, but the present invention is not limited thereto. .

Similar to the main body 1100, the cover part 1200 may also have a passage hole 1200H that provides a space through which a paraffin used in the embedding process passes. A plurality of passing holes 1200H may be formed, and may have various sizes / arrangements according to the characteristics of the tissue sample TS to be embedded. The passage hole 1200H may be circular, but the present invention is not limited thereto.

The cover portion 1200 includes a reagent penetrating tube 1210 surrounding the passage hole 1200H. The reagent penetrating tube 1210 is a tube whose top and bottom surfaces are open, and is fixed in a fixation agent such as formalin or paraffin used in embedding. It may provide a passage through which reagents enter and exit.

The flat surface of the cover portion 1200 and the reagent inlet tube 1210 may be integrally formed. The reagent penetrating tube 1210 may be disposed on the bottom surface of the cover unit 1200 with reference to FIG. 11. That is, as shown in FIG. 12, the reagent penetration tube 1210 may be located inside the main body 1100 while the cover 1200 covers the main body 1100.

According to another embodiment of the present invention, the reagent penetrating tube 1210 may be a hollow tube that becomes narrower in one direction. That is, with reference to FIGS. 11 and 12, the reagent penetration tube 1210 may be narrower in a downward direction.

According to another embodiment of the present invention, the reagent inlet tube 1210 may be in the form of a cone (cone) with the top and bottom open. That is, the reagent penetrating tube 1210 may have a pointed bottom. At this time, the width of the open lower surface may be 0.1cm ~ 0.5cm, but the present invention is not limited thereto.

FIG. 13 is a cross-sectional view taken along the line II-II ′ of the histological cassette 1000 of FIG. 12, FIG. 14 is an enlarged view of region B of FIG. 13, and FIG. 15 is a reagent penetration tube 1210. A cross-sectional view illustrating a method of infiltrating a reagent into a tissue specimen (TS).

13 to 15, the top and bottom surfaces of the reagent inlet tube 1210 are opened. Therefore, if the reagent is poured on the cover portion 1200, the reagent may flow through the reagent inlet tube 1210 into the body portion 1100.

According to another embodiment, the thickness of the reagent penetrating tube 1210 may decrease in one direction. Referring to FIG. 14, the thickness w _u4 of the relatively lower wall of the reagent inlet tube 1210 may be smaller than the thickness w _{u3 of the} wall of the upper side of the reagent penetrating tube 1210. That is, the cross section of the reagent penetrating tube 1210 may become sharper downward.

According to another embodiment, the reagent infiltration tube 1210 may have a pointed end to penetrate the tissue sample TS. Accordingly, the pointed end of the reagent penetrating tube 1210 may penetrate the surface of the tissue sample TS as shown in FIG. 15. The reagent penetrating tube 1210 may have a material for maintaining sharpness. For example, the reagent penetrating tube 1210 may include a material having high hardness, such as metal and stainless.

Since the lower end of the reagent penetrating tube 1210 is sharply formed, the reagent penetrating tube 1210 may penetrate a tissue sample TS made of mainly soft and soft adipose tissue such as breast tissue. Thus, the tissue sample TS may be stored in a histological cassette 1000 in a punctured state. In this state, when the biopsy cassette 1000 is placed in a tissue processor, the reagent is directly injected into the adipose tissue sample TS 'inside' through the reagent penetrating tube 1210. As a result, the penetration efficiency of the reagent can be improved to make the tissue specimen (TS) suitable for examination.

Meanwhile, the height of the reagent penetrating tube 1210 may be smaller than that of the main body 1100 so that the reagent penetrating tube 1210 does not completely penetrate the tissue sample TS. For example, the height of the reagent penetration tube 1210 may be half the height of the body portion 1100, but the present invention is not limited thereto.

According to another embodiment of the present invention, by infiltrating the reagent directly into the tissue sample (TS) through the reagent penetrating tube, the process of fixing, washing, dehydrating, and transparent the soft tissue sample (TS), such as adipose tissue, can be efficiently performed. In addition, it is possible to create a state suitable for embedding (embedding), microtome (microtome) to facilitate the biopsy.

According to the present invention, there is provided a cassette for histology and a method for histology using the same. In addition, the embodiments of the present invention can be applied to cassettes used for the production of tissue specimens for industrial histological examination.

Claims

A main body portion which is open at one side to hold a tissue sample; And

A cover part capable of opening and closing the main body part and having at least one through hole part formed therein; And

And a penetrating portion penetrating the through hole and coupled to the cover portion.
The method of claim 1,

Openings are formed at both sides of the penetrating portion and are formed in a hollow tube shape.
The method of claim 2,

The penetrating portion is formed to decrease in width in one direction, the histological cassette.
The method of claim 3,

The thickness of the penetrating portion decreases in one direction, the histological cassette.
The method of claim 1,

The penetrating portion,

Openings are formed on both sides, the penetration body is formed in a hollow tubular shape; And

And a base portion coupled to the penetrating body and fixing the penetrating body in position.
The method of claim 5,

The penetrating portion further includes a flow path portion coupled to the base portion.

And a penetrating body, the base portion and the flow path portion communicate with each other.
The method of claim 5,

The penetrating body is formed to decrease in width in one direction, the histological cassette.
One side is open to contain a tissue sample, the body portion is formed with at least one through hole; And

A cover part capable of opening and closing the main body part and coupled to the main body part; And

And a penetrating portion penetrating the through hole and coupled to the main body.
Placing the tissue sample in the body;

A fixing step of passing a penetrating part through a through hole formed in a cover part coupled to the main body part and penetrating the tissue sample with the penetrating part to fix the position of the tissue sample; And

And a penetrating step of injecting a reagent into the penetrating unit and penetrating the reagent into the tissue sample.
Placing the tissue sample in the body;

A fixing step of passing a penetrating part through a passage hole formed in the main body part and penetrating the tissue sample with the penetrating part to fix the position of the tissue sample; And

And a penetrating step of injecting a reagent into the penetrating unit and penetrating the reagent into the tissue sample.