WO2020028619A1 - Procédé de détection de micro-organismes sur la base d'un sparc - Google Patents
Procédé de détection de micro-organismes sur la base d'un sparc Download PDFInfo
- Publication number
- WO2020028619A1 WO2020028619A1 PCT/US2019/044592 US2019044592W WO2020028619A1 WO 2020028619 A1 WO2020028619 A1 WO 2020028619A1 US 2019044592 W US2019044592 W US 2019044592W WO 2020028619 A1 WO2020028619 A1 WO 2020028619A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sbp
- labeled
- activator
- sbps
- species
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Definitions
- the invention provides a rapid homogeneous immunoassay detection method for microorganisms (e.g., living bacteria).
- microorganisms e.g., living bacteria.
- the immunoassay is a simple mix-and-read format that can generate results in ⁇ 20 minutes and can be used for, e.g., food safety testing, water safety testing, and any industry where microbial contamination is a concern (such as hospitals).
- the immunoassay could be used as a rapid, up-front diagnostic that could later be confirmed by traditional methods; for example, as an initial, quick method to determine if a bacterial infection is Gram-positive or Gram-negative.
- the sample can be any suitable sample, including one that is suspected of containing microorganisms (e.g., bacteria).
- the sample can be obtained from any suitable source, including, but not limited to, a patient, a liquid, any kind of food, a drug, or a swab.
- Typical samples which can be used in the methods of the invention include bodily fluids such as blood, which can be anticoagulated blood as is commonly found in collected blood specimens, plasma, serum, urine, stool, sputum, mucus, semen, saliva, cell cultures, tissue extracts, body swabs, and the like.
- Other types of samples include solvents, seawater, industrial water samples, food samples, and environmental samples such as soil, water, and plant materials.
- the microorganisms are bacteria (e.g., living bacteria, spores, etc), such as Gram-positive or Gram-negative bacteria. Any bacteria can be detected including, but not limited to, bacteria of the following genera: Corynebacterium,
- Staphylococcus e.g., Methicillin-resistant Staphylococcus aureus, i.e., MRSA
- Streptococcus Clostridia, Neisseria, Campylobacter, Enterobacter, Escherichia, Bacteriodes, Burkholderia, Haemophilus, Klebsiella, Legionella, Proteus, Pseudomonas, Salmonella, Shigella, Vibrio, Yersinia, and/or Mycoplasma.
- the sbp is a molecule or a complex, including biological molecules and complexes, that has specific binding affinity for another substance including DNA, RNA, oligonucleotides, antibodies, antibody fragments, antibody-DNA chimeras, antigens, haptens, proteins, peptides, lectins, avidin, streptavidin, and biotin.
- Sbps can be conjugated to one or more molecules of either an activator or a chemiluminescent compound.
- step (a) chemiluminescent compound
- enhancers include phenolic compounds and aromatic amines known to enhance other peroxidase reactions as described in U.S. Patent Nos. 5,171,668 and 5,206,149.
- Substituted and unsubstituted arylboronic acid compounds and their ester and anhydride derivatives as disclosed in U.S. Patent 5,512,451 are also considered to be within the scope of enhancers useful in the present invention.
- antimicrobial agents include, but are not limited to, antibacterial (antibiotic), antifungals, and antiviral agents.
- the antimicrobials agents are antibacterial or antifungal agents.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un procédé de détection de la présence de micro-organismes (par exemple, des bactéries, des virus et champignons) dans un échantillon, ainsi qu'un procédé de détermination de la sensibilité de microorganismes à des substances antimicrobiennes (par exemple, antibiotiques, antifongiques ou antivirales).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862713200P | 2018-08-01 | 2018-08-01 | |
US62/713,200 | 2018-08-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2020028619A1 true WO2020028619A1 (fr) | 2020-02-06 |
Family
ID=67742966
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2019/044592 WO2020028619A1 (fr) | 2018-08-01 | 2019-08-01 | Procédé de détection de micro-organismes sur la base d'un sparc |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2020028619A1 (fr) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5171668A (en) | 1988-09-30 | 1992-12-15 | Fujirebio Inc. | Method of the chemiluminescence assay of the activity of peroxidase |
US5206149A (en) | 1989-04-28 | 1993-04-27 | Toray Industries, Inc. | Method of high sensitivity luminescence analysis |
US5512451A (en) | 1993-04-01 | 1996-04-30 | British Technology Group Limited | Enhancement of chemiluminescent reactions |
US7732153B2 (en) | 2006-05-09 | 2010-06-08 | Beckman Coulter, Inc. | Nonseparation assay methods |
US7799534B2 (en) | 2006-05-09 | 2010-09-21 | Beckman Coulter, Inc. | Nonseparation assay methods |
US8124392B2 (en) | 2002-05-01 | 2012-02-28 | Vertex Pharmaceuticals Incorporated | Crystal structure of Aurora-2 protein and binding pockets thereof |
US9029092B2 (en) | 2009-02-27 | 2015-05-12 | Beckman Coulter, Inc. | Solution phase homogeneous assays |
-
2019
- 2019-08-01 WO PCT/US2019/044592 patent/WO2020028619A1/fr active Application Filing
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5171668A (en) | 1988-09-30 | 1992-12-15 | Fujirebio Inc. | Method of the chemiluminescence assay of the activity of peroxidase |
US5206149A (en) | 1989-04-28 | 1993-04-27 | Toray Industries, Inc. | Method of high sensitivity luminescence analysis |
US5512451A (en) | 1993-04-01 | 1996-04-30 | British Technology Group Limited | Enhancement of chemiluminescent reactions |
US8124392B2 (en) | 2002-05-01 | 2012-02-28 | Vertex Pharmaceuticals Incorporated | Crystal structure of Aurora-2 protein and binding pockets thereof |
US7732153B2 (en) | 2006-05-09 | 2010-06-08 | Beckman Coulter, Inc. | Nonseparation assay methods |
US7923214B2 (en) | 2006-05-09 | 2011-04-12 | Beckman Coulter, Inc. | Nonseparation assay methods |
US7923213B2 (en) | 2006-05-09 | 2011-04-12 | Beckman Coulter, Inc. | Nonseparation assay methods |
US8012705B2 (en) | 2006-05-09 | 2011-09-06 | Beckman Coulter, Inc. | Nonseparation assay methods |
US8076092B2 (en) | 2006-05-09 | 2011-12-13 | Beckman Coulter, Inc. | Nonseparation assay methods |
US7799534B2 (en) | 2006-05-09 | 2010-09-21 | Beckman Coulter, Inc. | Nonseparation assay methods |
US8377647B2 (en) | 2006-05-09 | 2013-02-19 | Beckman Coulter, Inc. | Nonseparation assay methods |
US9029092B2 (en) | 2009-02-27 | 2015-05-12 | Beckman Coulter, Inc. | Solution phase homogeneous assays |
US20150212005A1 (en) | 2009-02-27 | 2015-07-30 | Beckman Coulter, Inc. | Solution phase homogeneous assays |
US10036708B2 (en) * | 2009-02-27 | 2018-07-31 | Beckman Coulter, Inc. | Solution phase homogeneous assays |
Non-Patent Citations (5)
Title |
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CI ET AL., MIKROCHEM. J., vol. 52, 1995, pages 257 - 62 |
DUAN NUO ET AL: "Simultaneous detection of pathogenic bacteria using an aptamer based biosensor and dual fluorescence resonance energy transfer from quantum dots to carbon nanoparticles", MIKROCHIMICA ACTA, SPRINGER VERLAG, VIENNA, AT, vol. 182, no. 5, 15 November 2014 (2014-11-15), pages 917 - 923, XP035468458, ISSN: 0026-3672, [retrieved on 20141115], DOI: 10.1007/S00604-014-1406-3 * |
MARK J CAMERON ET AL: "An Automated Pharmacokinetic Immunoassay Using Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL) for Human IgG Drugs in Preclinical Species", 1 January 2017 (2017-01-01), pages 1 - 3, XP055629457, Retrieved from the Internet <URL:https://www.andrewalliance.com/wp-content/uploads/2017/05/AN_Automated_Pharmacokinetic_Immunoassay_HD-1.pdf> [retrieved on 20191007] * |
SHIJIA WU ET AL: "Simultaneous Aptasensor for Multiplex Pathogenic Bacteria Detection Based on Multicolor Upconversion Nanoparticles Labels", ANALYTICAL CHEMISTRY, vol. 86, no. 6, 6 March 2014 (2014-03-06), US, pages 3100 - 3107, XP055458777, ISSN: 0003-2700, DOI: 10.1021/ac404205c * |
Y. S. KIM ET AL: "High-Throughput and Facile Assay of Antimicrobial Peptides Using pH-Controlled Fluorescence Resonance Energy Transfer", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 50, no. 10, 27 September 2006 (2006-09-27), US, pages 3330 - 3335, XP055630537, ISSN: 0066-4804, DOI: 10.1128/AAC.00455-06 * |
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