WO2020026031A3 - Methods and systems for target enrichment - Google Patents

Methods and systems for target enrichment Download PDF

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Publication number
WO2020026031A3
WO2020026031A3 PCT/IB2019/000888 IB2019000888W WO2020026031A3 WO 2020026031 A3 WO2020026031 A3 WO 2020026031A3 IB 2019000888 W IB2019000888 W IB 2019000888W WO 2020026031 A3 WO2020026031 A3 WO 2020026031A3
Authority
WO
WIPO (PCT)
Prior art keywords
systems
methods
target enrichment
enrichment
target
Prior art date
Application number
PCT/IB2019/000888
Other languages
French (fr)
Other versions
WO2020026031A2 (en
Inventor
Shirong YU
Joanne MASON
Sidong LIU
Jason MELLAD
Original Assignee
Cambridge Epigenetix Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Epigenetix Limited filed Critical Cambridge Epigenetix Limited
Publication of WO2020026031A2 publication Critical patent/WO2020026031A2/en
Publication of WO2020026031A3 publication Critical patent/WO2020026031A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Provided herein are methods and systems for target enrichment. The methods and systems as described herein may provide an approach for target enrichment with high efficiency and selectivity.
PCT/IB2019/000888 2018-08-02 2019-08-02 Methods and systems for target enrichment WO2020026031A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862713734P 2018-08-02 2018-08-02
US62/713,734 2018-08-02

Publications (2)

Publication Number Publication Date
WO2020026031A2 WO2020026031A2 (en) 2020-02-06
WO2020026031A3 true WO2020026031A3 (en) 2020-03-19

Family

ID=68425156

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2019/000888 WO2020026031A2 (en) 2018-08-02 2019-08-02 Methods and systems for target enrichment

Country Status (1)

Country Link
WO (1) WO2020026031A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113249446A (en) * 2021-04-13 2021-08-13 中山大学 Quantitative method of 5hmC level of whole genome based on nucleic acid isothermal amplification and application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005047542A1 (en) * 2003-10-16 2005-05-26 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Treatments for inhibiting development and progression of nevi and melanoma having braf mutations
US20090208941A1 (en) * 2005-07-19 2009-08-20 Epigenomics Ag Method for investigating cytosine methylations in dna
GB2523919A (en) * 2012-11-06 2015-09-09 New England Biolabs Inc Mapping cytosine modifications
WO2016034908A1 (en) * 2014-09-05 2016-03-10 Cambridge Epigenetix Ltd Methods for detection of nucleotide modification
WO2016080750A1 (en) * 2014-11-18 2016-05-26 사회복지법인 삼성생명공익재단 Gene panel for detecting cancer genome mutant

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005047542A1 (en) * 2003-10-16 2005-05-26 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Treatments for inhibiting development and progression of nevi and melanoma having braf mutations
US20090208941A1 (en) * 2005-07-19 2009-08-20 Epigenomics Ag Method for investigating cytosine methylations in dna
GB2523919A (en) * 2012-11-06 2015-09-09 New England Biolabs Inc Mapping cytosine modifications
WO2016034908A1 (en) * 2014-09-05 2016-03-10 Cambridge Epigenetix Ltd Methods for detection of nucleotide modification
WO2016080750A1 (en) * 2014-11-18 2016-05-26 사회복지법인 삼성생명공익재단 Gene panel for detecting cancer genome mutant

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHUN-XIAO SONG ET AL: "Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine", NATURE BIOTECHNOLOGY, vol. 29, no. 1, 1 January 2011 (2011-01-01), pages 68 - 72, XP055012325, ISSN: 1087-0156, DOI: 10.1038/nbt.1732 *
YUJING LI ET AL: "Selective Capture of 5-hydroxymethylcytosine from Genomic DNA", JOURNAL OF VISUALIZED EXPERIMENTS, no. 68, 5 October 2012 (2012-10-05), XP055559393, DOI: 10.3791/4441 *

Also Published As

Publication number Publication date
WO2020026031A2 (en) 2020-02-06

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