WO2019242008A1 - Nouveau diluteur intégré d'échantillon et procédé de dilution d'échantillon - Google Patents

Nouveau diluteur intégré d'échantillon et procédé de dilution d'échantillon Download PDF

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Publication number
WO2019242008A1
WO2019242008A1 PCT/CN2018/092438 CN2018092438W WO2019242008A1 WO 2019242008 A1 WO2019242008 A1 WO 2019242008A1 CN 2018092438 W CN2018092438 W CN 2018092438W WO 2019242008 A1 WO2019242008 A1 WO 2019242008A1
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WO
WIPO (PCT)
Prior art keywords
capillary
sample
protective sleeve
open end
airbag
Prior art date
Application number
PCT/CN2018/092438
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English (en)
Chinese (zh)
Inventor
马云天
马荣华
Original Assignee
江苏科华医疗器械科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 江苏科华医疗器械科技有限公司 filed Critical 江苏科华医疗器械科技有限公司
Publication of WO2019242008A1 publication Critical patent/WO2019242008A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

Definitions

  • the present disclosure belongs to the technical field of experimental equipment, and particularly relates to a novel integrated sample diluter and a sample dilution method.
  • samples are often diluted.
  • a pipette is generally used to measure the diluent, and then a sample is taken, and the sample is added to the diluent. After the two are mixed evenly, the diluent is sucked. And waste experimental supplies.
  • the purpose of the present disclosure includes providing a new integrated sample diluter to solve at least one technical problem existing in the background art.
  • the novel integrated sample diluter includes a balloon, a capillary tube, and a protective sleeve.
  • One end of the capillary tube is a sealed end, and the other end of the capillary tube is an open end.
  • the sealed end of the capillary tube is inserted into the balloon.
  • the open end of the capillary is inserted into a protective sleeve, the capillary is provided with micropores and a breakable line, the breakable line is close to the sealed end of the capillary, and the microhole is close to the open end of the capillary.
  • the inner diameter of the capillary is 0.5 to 2.0 mm.
  • the distance between the micropore and the open end of the capillary is 5-30 mm.
  • the distance between the foldable line and the sealed end of the capillary is 5-25 mm.
  • the airbag includes a cavity and a constriction connected to the cavity, and the breakable line of the capillary tube is located inside the airbag, and is substantially in a seam with the joint between the cavity and the constriction. On a horizontal plane.
  • the necking is an elastic necking and the set is tightly arranged on the sealed end of the capillary.
  • the airbag is filled with a diluent.
  • the sealed end of the air bag is provided with a guide portion configured to guide the sealed end of the capillary tube into the air bag.
  • the opening diameter of the protective sleeve is larger than the outer diameter of the open end of the capillary tube.
  • microhole is located inside the protective sleeve.
  • non-open end of the protective sleeve is a flat bottom and is configured to stand on a workbench.
  • the sample dilution method provided by the present disclosure uses the above-mentioned new integrated sample diluter to perform a dilution process on a sample, and includes the following steps:
  • S50 Insert the open end of the capillary into the protective sleeve, and squeeze the balloon, so that the sample in the capillary and the diluent in the balloon are squeezed into the protective sleeve at the same time.
  • step S10 after the diluent is loaded into the airbag, the method further includes inverting the new integrated sample diluter so that the airbag is down and the protective sleeve is up.
  • the method further includes setting the capillary upright so that the balloon is on top and the open end of the capillary is on the bottom.
  • the microhole is also located in the protective sleeve.
  • the method further includes mixing the sample and the diluent in the protective sleeve.
  • the new integrated sample diluter When using the new integrated sample diluter, first the end of the diluter with the bladder facing down and the end with the protective sleeve facing up, break the capillary along the easy-to-break line, and remove the protective sleeve; In the tilted state, the open end of the capillary is contacted with the sample liquid to be sucked, and the sample is sucked into the capillary by capillary action; when the sample liquid level reaches the micropore and closes the micropore, the suction is stopped, and then the diluent has a balloon. One end is up. At this time, all the diluent in the balloon is concentrated on the bottom of the balloon.
  • the open end of the capillary is inserted into the protective sleeve, and the balloon is squeezed so that the diluent and the sample in the balloon are discharged to the capillary.
  • the liquid in the protective sleeve is mixed uniformly to obtain a diluent.
  • the new integrated sample diluter and sample dilution method realize the operation of measuring the diluent, sampling the sample, diluting the sample and diluting the sample in a laboratory equipment in sequence. It simplifies the tedious process in the previous sample dilution process, improves the experimental efficiency, and saves experimental consumables, thereby reducing the experimental cost.
  • FIG. 1 is a schematic structural diagram of a new integrated sample diluter provided by the present disclosure
  • FIG. 2 is a schematic structural diagram of a new integrated sample diluter provided by the present disclosure after the closed end of a capillary tube is broken;
  • FIG. 3 is a schematic structural diagram of a new integrated sample diluter provided by the present disclosure after a protective sleeve is removed from a capillary;
  • FIG. 4 is a schematic structural diagram of a new integrated sample diluter provided by the present disclosure before preparing to squeeze a sample and a diluent into a protective sleeve.
  • 1-airbag 2-capillary tube; 3-protective sleeve; 4-sealed end; 5-open end; 6-microporous; 7-easy line.
  • orientations or positional relationships indicated by the terms “up”, “down”, and “horizontal” are based on the orientations or positional relationships shown in the drawings, and are merely for the convenience of describing the present invention.
  • the disclosure and simplified description do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as a limitation on the present disclosure.
  • the present disclosure provides a new type of integrated sample diluter, including a balloon 1, a capillary 2, and a protective sleeve 3.
  • one end of the capillary 2 is a sealed end 4, and the other end of the capillary 2 is an opening.
  • End 5 wherein the sealed end 4 of the capillary 2 is inserted into the airbag 1, the open end 5 of the capillary 2 is inserted into the protective sleeve 3, and the capillary 2 is provided with a microhole 6 and a foldable line 7, which is close to the seal of the capillary 2 End 4, the micropore 6 is close to the open end 5 of the capillary 2.
  • the inner diameter of the capillary 2 may be 0.5 to 2.0 mm.
  • This setting enables the sample to be reliably aspirated, thereby improving the accuracy of the sample measurement process.
  • the distance between the micropore 6 and the open end 5 of the capillary 2 is 5-30 mm.
  • the distance between the foldable line 7 and the sealed end 4 of the capillary tube 2 is 5 to 25 mm.
  • This arrangement allows sufficient length for the sealed end 4 so that the operator can easily break the capillary 2 from the breakable line 7.
  • the arrangement of the easy-break line 7 reduces the difficulty of breaking the capillary 7, thereby ensuring the working reliability of the new integrated sample diluter of this embodiment.
  • the airbag 1 includes a receiving cavity and a constriction connected to the receiving cavity.
  • the flexible line 7 of the capillary tube 2 is located inside the airbag 1 and is between the cavity and the constriction.
  • the seams are basically on a horizontal plane.
  • the experimenter needs to break the capillary 2 from the foldable line 7, he can refer to the position of the chamber of the airbag 1 and the constriction seam, and then apply force to the capillary 2 from here to break the capillary 2.
  • This arrangement improves the pertinence of the force applied when the capillary tube 2 is broken, so that it can be quickly broken, thereby further improving the experimental efficiency.
  • the above-mentioned constriction may be an elastic constriction, which is used to be sleeved on the sealed end 4 of the capillary tube 2. In this arrangement, after the capillary tube 2 is inserted into the airbag 1, there is almost no gap between the capillary tube 2 and the opening of the airbag 1, thereby effectively preventing the outflow of the diluent.
  • a diluent is contained in the airbag 1, and the volume of the diluent is set in advance.
  • the airbag 1 may be provided as a transparent airbag, and a corresponding scale may be provided on the outside thereof.
  • a transparent airbag enables the experimenter to know the diluent content involved in the dilution process in real time, and during the process of squeezing the diluent into the protective sleeve 3, the experimenter can also observe the remaining amount of diluent in the airbag 1 to The diluent is further squeezed into the protective sleeve 3. Not only the diluent residue in the airbag 1 is reduced, but also the accuracy of the dilution is improved, thereby ensuring the experimental effect.
  • the sealed end 4 of the airbag 1 may further be provided with a guide portion for guiding the sealed end 4 of the capillary tube 2 to be inserted into the airbag 1.
  • This arrangement enables the closed end of the capillary tube 2 to be quickly inserted into the balloon 1 when assembling the new integrated sample diluter, further improving the experimental efficiency.
  • the guide portion on the sealed end 4 may be arc-shaped.
  • the opening diameter of the protective sleeve 3 is larger than the outer diameter of the open end 5 of the capillary tube 2.
  • the micropore 6 is also located inside the protective sleeve 3.
  • This arrangement avoids to a certain extent the sample overflowing from the microwell 6 to the outside of the protective sleeve 3 during the sample dilution process, which not only reduces waste but also reduces pollution to the experimental environment.
  • the non-open end of the protective sleeve 3 may be a flat bottom for standing on a workbench.
  • This embodiment also provides a sample dilution method.
  • the sample dilution method uses the above-mentioned new integrated sample diluter to dilute a sample, including the following steps:
  • S50 Insert the open end 5 of the capillary tube 2 into the protective sleeve 3, and squeeze the balloon 1 so that the sample in the capillary tube 2 and the diluent in the balloon 1 are squeezed into the protective sleeve 3 at the same time.
  • the sample dilution method further includes: after filling the diluent into the airbag 1, inverting the new integrated sample diluter so that the airbag 1 is down and the protective sleeve 3 is up .
  • the method further includes placing the capillary tube 2 upright so that the balloon 1 is on top and the open end 5 of the capillary tube 2 is on the bottom, as shown in FIG. 4.
  • This arrangement enables the diluent in the airbag 1 to be almost completely squeezed into the protective sleeve 3 under the effect of its own gravity, thereby reducing the residual of the diluent in the airbag 1 and thereby ensuring the reliability of the dilution.
  • the sample and the diluent in the protective sleeve 3 can also be mixed. This setting ensures the uniformity of the mixing of the two, thereby ensuring the dilution effect.
  • a new type of integrated sample diluter and sample dilution method realizes the operation of sequentially measuring a diluent, a sample, a sample, and a sample after dilution in an experimental device. It is easy to operate, which not only simplifies the tedious process of the previous sample dilution process, improves the experimental efficiency, but also saves the experimental consumables, thereby reducing the experimental cost.

Abstract

L'invention concerne un nouveau diluteur intégré d'échantillon comprenant un coussin d'air (1), un tube capillaire (2) et un manchon protecteur (3). Une extrémité du tube capillaire (2) est une extrémité scellée et l'autre extrémité du tube capillaire (2) est une extrémité ouverte, l'extrémité scellée du tube capillaire (2) étant insérée dans le coussin d'air (1), l'extrémité ouverte du tube capillaire (2) étant insérée dans le manchon protecteur (3) et le tube capillaire (2) étant pourvu d'un micropore (6) et d'une ligne de pliage facile (7). La ligne de pliage facile (7) est proche de l'extrémité scellée du tube capillaire (2). Le micropore (6) est proche de l'extrémité ouverte du tube capillaire (2). L'adoption du nouveau diluteur intégré d'échantillon lors de la dilution d'un échantillon permet à un utilisateur de mesurer séquentiellement un diluant, de mesurer l'échantillon, de diluer l'échantillon et de mesurer l'échantillon dilué avec un seul élément d'équipement expérimental, ce qui permet d'augmenter l'efficacité et d'économiser le matériel utilisé lors d'une expérience.
PCT/CN2018/092438 2018-06-19 2018-06-22 Nouveau diluteur intégré d'échantillon et procédé de dilution d'échantillon WO2019242008A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201810628679.7A CN108507860A (zh) 2018-06-19 2018-06-19 一种新型一体化样品稀释器
CN201810628679.7 2018-06-19

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Publication Number Publication Date
WO2019242008A1 true WO2019242008A1 (fr) 2019-12-26

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WO (1) WO2019242008A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110196182A (zh) * 2019-04-23 2019-09-03 江苏科华医疗器械科技有限公司 一种封闭式采血稀释器及其使用方法

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CN101291620A (zh) * 2005-08-04 2008-10-22 惠普开发有限公司 收集及稀释液体样品的方法和装置
CN101957273A (zh) * 2009-07-15 2011-01-26 英科新创(厦门)科技有限公司 收集和按固定比例稀释微量液体样本的装置及其使用方法
CN102072949A (zh) * 2010-11-11 2011-05-25 郝书顺 一种一体化定量采样加试剂装置
WO2012133605A1 (fr) * 2011-03-31 2012-10-04 積水メディカル株式会社 Extrémité de pipette
CN205209809U (zh) * 2015-10-27 2016-05-04 光景生物科技(苏州)有限公司 一种微量液体取样加样装置及其采血套件
CN206362581U (zh) * 2016-12-28 2017-07-28 天津中新科炬生物制药有限公司 一种液体样本采集装置

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DE19716073A1 (de) * 1997-04-17 1998-10-22 Boehringer Mannheim Gmbh Dosiervorrichtung zur Abgabe kleiner Flüssigkeitsmengen
FI20095230A0 (fi) * 2009-03-09 2009-03-09 Thermo Fisher Scientific Oy Laimennuskärki
JP2012026997A (ja) * 2010-07-22 2012-02-09 Aska Special Laboratory Co Ltd 毛細管による微量検体採取・希釈・分注方法及び器具
US20160288118A1 (en) * 2013-03-20 2016-10-06 Siemens Healthcare Diagnostics Inc. Pipette components useful for medical diagnostics
CN105498877A (zh) * 2014-12-23 2016-04-20 马荣华 定量移液管
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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101291620A (zh) * 2005-08-04 2008-10-22 惠普开发有限公司 收集及稀释液体样品的方法和装置
CN101957273A (zh) * 2009-07-15 2011-01-26 英科新创(厦门)科技有限公司 收集和按固定比例稀释微量液体样本的装置及其使用方法
CN102072949A (zh) * 2010-11-11 2011-05-25 郝书顺 一种一体化定量采样加试剂装置
WO2012133605A1 (fr) * 2011-03-31 2012-10-04 積水メディカル株式会社 Extrémité de pipette
CN205209809U (zh) * 2015-10-27 2016-05-04 光景生物科技(苏州)有限公司 一种微量液体取样加样装置及其采血套件
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