WO2019218735A1 - 一种检测仪光学系统 - Google Patents

一种检测仪光学系统 Download PDF

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Publication number
WO2019218735A1
WO2019218735A1 PCT/CN2019/075426 CN2019075426W WO2019218735A1 WO 2019218735 A1 WO2019218735 A1 WO 2019218735A1 CN 2019075426 W CN2019075426 W CN 2019075426W WO 2019218735 A1 WO2019218735 A1 WO 2019218735A1
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Prior art keywords
light
filter
detector
path
optical system
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PCT/CN2019/075426
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English (en)
French (fr)
Inventor
罗继全
罗秦
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三诺生物传感股份有限公司
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Publication of WO2019218735A1 publication Critical patent/WO2019218735A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts

Definitions

  • the present invention relates to the field of biochemical analysis and detection technology, and specifically refers to a detector optical system.
  • the principle of biochemical detection is colorimetric, turbid, and excited fluorescence. In order to detect more items, many wavelengths are needed. This requires dividing the complex light into many monochromatic lights.
  • the commonly used splitting methods are as follows: 1. Rotating filter wheel splitting scheme, generally used for pre-split, placing a circumferential array of filters of different wavelengths in the mounting wheel, which wavelength detection is needed The rotating wheel is turned to the corresponding wavelength. This scheme is simple and easy to implement, but the efficiency is low and the device volume is large. 2.
  • the spectroscopic array splitting scheme is generally used in the post-split mode to split the complex color light of the reaction cup into multiple channels.
  • Each channel is placed with a filter of corresponding wavelength for monochrome; this scheme has no transmission mechanism, and has high reliability and relatively compact structure; 3.
  • Grating spectroscopic scheme generally used for post-split scheme, grating spectroscopic can be divided into one-time Many wavelengths are compact and efficient, but they also have the disadvantages of high cost, high positioning requirements, and high stray light.
  • biochemical detection instruments can achieve colorimetric detection and transmission turbidity detection according to analysis requirements, can not achieve scattering turbidimetric detection, but the transmission turbidity signal-to-noise ratio and sensitivity are not high turbidity, and biochemical instruments are not Support for excitation fluorescence detection, less functionality, can not meet the needs of multi-project analysis and high-accuracy applications, therefore, 5 see technology still needs to be improved and developed.
  • the object of the present invention is to provide a three-dimensional optical detection with reasonable structure, which can realize transmission, scattering and excitation of fluorescence, and to meet the requirements of concentration detection of various objects. system.
  • a detector optical system includes an illumination source, a light-passing object to be tested, a filter mechanism, and a probe a filter, the filter mechanism is disposed on the incident light path between the illumination source and the light-transmitting object to be tested, and the first light-splitting mechanism and the corresponding detector are disposed on the transmitted light path of the light-transmitting object to be tested, and the detector comprises multiple groups.
  • a narrow band filter and an optical signal receiver; a second beam splitting mechanism and a corresponding detector are disposed on the refracted light path of the light through the object to be tested, and the detector comprises an optical signal receiver and a fluorescent receiver.
  • the filter mechanism includes a filter wheel, and the illumination light source and the filter wheel are sequentially arranged on an incident light path of the light-emitting object to be tested, and the filter wheel is provided with an exit pass.
  • a hole and a plurality of monochromatic filters the exit through hole and the plurality of monochromatic filters are distributed on the same circumferential track, and the rotating circumferential track intersects with the incident optical path of the light passing object to make the outgoing through hole and the plurality of monochrome
  • the filters can be paired with the illumination source separately.
  • the first beam splitting mechanism includes a plurality of beam splitters, and the plurality of beam splitters are arranged at intervals on the transmitted light path of the light-transmitting object to be tested; and the reflected light paths of any of the beamsplitters are A narrow band filter and a corresponding optical signal receiver are provided, and the plurality of narrow band filters respectively correspond to different monochromatic wavelength ranges to filter the optical signal reflected by the beam splitter.
  • the reflected light paths of the adjacent two beamsplitters in the spectroscopic array are opposite in direction.
  • the second beam splitting mechanism includes a beam splitter and a light filter, and the beam splitter and the light signal receiver are disposed along a refracting light path of the light through the object to be tested, and the fluorescent receiver is disposed on the beam splitter.
  • a fluorescent filter is disposed between the beam splitter and the fluorescent receiver.
  • the beam splitter may be any one of a dichroic mirror or a neutral beam splitter.
  • the optical path uses an optical fiber as a light propagation medium.
  • a concentrating array is disposed on the incident light path between the illumination source and the filter mechanism, and the concentrating array is composed of a plurality of lenses arranged in sequence, and the illumination source forms a collimation under the action of the concentrating array. The light is projected onto the light-through object to be tested.
  • a lens is disposed on the optical path between the light-emitting object to be tested and the first beam splitting mechanism, the second beam splitting mechanism, and the filter mechanism.
  • the beneficial effects of the present invention are as follows:
  • the structure of the invention is reasonable, and the collimated light generated by the illumination source through the concentrating array composed of the lens enters the light-passing object to be tested after the screening of the filter mechanism, and the transmitted and scattered light signals are generated.
  • the light signal is transmitted through the first and second beam splitting mechanisms, and the light signals are respectively received by the detector after the single-color filtering of the narrow-band filter and the fluorescent filter of different wavelengths, thereby realizing a multi-detection combination of a system, and the overall structure is compact.
  • the cost is not high and the use is flexible, which can meet the needs of most current optical testing experiments.
  • FIG. 2 is a partial structural schematic view of a filter mechanism of the present invention.
  • a detector optical system includes an illumination source 1, a light-passing object to be tested 2, a filter mechanism 3, and a detector, and the filter mechanism 3 is disposed on the illumination source 1 and the light-emitting object to be tested.
  • a first optical splitting means and a corresponding detector are disposed on the transmitted optical path between the two objects, the detector includes a plurality of sets of narrow band filters 41 and an optical signal receiver 43; A second beam splitting mechanism and a corresponding detector are disposed on the refracting light path of the light-transmitting object 2, the detector includes an optical signal receiver 43 and a fluorescent receiver 44; and the illuminating light source 1 generates light into the light-passing object to be tested 2 Transmission and scattering phenomenon, wherein the transmitted light passes through the first light splitting mechanism along the optical path, and the first light splitting mechanism sequentially reflects the optical signals of different wavelengths to the corresponding detectors, and receives corresponding information through the plurality of optical signal receivers 43 to achieve colorimetric Detecting, and the scattered light passes through the second beam splitting mechanism along the optical path, and the corresponding scattered light signal receiver 43 and fluorescent receiver 44 are used to implement the turbidity and excitation fluorescence item detection.
  • the incident light path and the transmitted light path are on the same straight line passing through the light-emitting object 2, so that the light emitted by the light source 1 is projected on the light-through object 2 along the incident light path, and the light is measured in the light-passing
  • a transmitted light signal and a scattered light signal are generated on the object 2, wherein the transmitted light signal continues to propagate along the transmitted light path, and the scattered light signal propagates along the refracted light path
  • the refracted light path generally refers to the incident light path and the transmitted light path except the light passing object 2
  • the arbitrary scattering direction, the refracted light path described in the present case is perpendicular to the transmitted light path of the light-emitting object 2 to obtain the strongest scattered light signal and the optimal structural layout, and the refracted light path may be the transmitted light path as the axis Any radial.
  • the filter mechanism 3 includes a filter wheel 31, and the illumination source 1 and the filter wheel 31 are sequentially arranged on the incident light path of the light-to-be-tested object 2, and the filter The wheel 31 is provided with an exit through hole 32 and a plurality of monochromatic filters 33.
  • the exit through hole 32 and the plurality of monochromatic filters 33 are distributed on the same circumferential track, and the rotating circumferential track and the light passing object 2 are The incident light paths intersect such that the exit through holes 32 and the plurality of monochromatic filters 33 are respectively paired with the illumination light source 1; the filter mechanism 3 is configured to filter the spectral range of the illumination source 1 according to the detection item, and the exit through holes 32 Corresponding to the full-wavelength ray for colorimetric detection, when the corresponding monochromatic filter 33 is rotated with the filter wheel 31 to the incident optical path, only monochromatic light in the wavelength range of the monochromatic filter 33 can be allowed to pass through. And irradiating the light-transmitting object to be tested 2 through the incident light path, thereby filtering the excess light signal to reduce interference with the detection result.
  • the first beam splitting mechanism includes a plurality of beam splitters 4, and the plurality of beam splitters 4 are arranged at intervals on the transmitted light path of the light-emitting object 2 to form a light array, and the plurality of beam splitters 4 are connected to the light-emitting object 2
  • the transmitted light path is disposed at an angle of 45°; the narrowed-band filter 41 and the corresponding optical signal receiver 43 are disposed on the reflected light path of any of the beam splitters 4, and the plurality of narrow-band filters 41 respectively correspond to different single colors.
  • the wavelength range is to filter the light signal reflected by the beam splitter 4; the light penetrating from the light-emitting object 2 passes through a plurality of beam splitters 4 in the beam splitting array, which are reflected by the beam splitter 4 and output after filtering by the narrow band filter 41.
  • the optical signal receiver 43 is supplied, and the remaining wavelengths of light continue to penetrate the next beam splitter 4 and reflect the corresponding optical signals.
  • the number of the beam splitters 4 of the present scheme can be increased and decreased according to the detection requirements, but the spectroscopic array should include at least The beam splitter 4 and the narrow band filter 41 of the usual wavelengths of 340, 405, 505, 546, 578, 630, 660, and 700 are used.
  • the beam splitter 4 may be any one of a dichroic mirror or a neutral beam splitter.
  • the dichroic mirror refers to a spectroscope 4 that reflects only light of a specific wavelength. When a wavelength type is required, the second type is used.
  • the color mirror can increase the signal value of the long wavelength wavelength; the neutral beam splitter can be used when the number of the spectral array is small, thereby reducing the component type to reduce the structural cost when detecting a specific range.
  • the reflected light paths of the two adjacent beamsplitters 4 in the splitting array are opposite.
  • the adjacent two beamsplitters 4 are disposed at an angle of 90°.
  • the detectors on both sides of the first beam splitting mechanism are symmetrically arranged, which can effectively reduce and compact the overall structure of the detector, and improve the optical signal transmission efficiency.
  • the second beam splitting mechanism includes a beam splitter 4 and a phosphor filter 42.
  • the beam splitter 4 and the light signal receiver 43 are disposed along a refracting optical path of the light-emitting object 2, and the fluorescent receiver 44 is disposed on the beam splitter.
  • the fluorescent filter 42 is disposed between the beam splitter 4 and the fluorescent receiver 44, the light scattered from the light-passing object to be tested 2 enters the second beam splitting mechanism, and the second beam splitting mechanism is used
  • the scattered light turbidity detection and the excitation fluorescence detection, the scattered light passes through the beam splitter 4 and is received by the corresponding optical signal receiver 43, and the light reflected by the beam splitter 4 passes through the fluorescent filter 42 to cut off the excitation light output to the fluorescent receiver. 44.
  • the optical path may use the optical fiber 5 as a light propagation medium, and the optical path includes an incident optical path between the illumination light source 1 and the light-to-test object 2, and between the light-to-test object 2 and the first light-splitting mechanism. a condensed optical path between the transmitted light path, the light-passing analyte 2 and the second light-splitting mechanism; the optical fiber 5 is used to construct an optical path of the system to connect the main body components, thereby isolating the heat source, maintaining the light propagation direction, and reducing signal loss. The role.
  • the illumination source 1 may be a south lamp or an LED light source, and a concentrating array is disposed on the incident light path between the illumination source 1 and the filter mechanism 3.
  • the concentrating array is composed of a plurality of lenses 12 arranged in sequence, the lens 12 converges and calibrates the light by focusing, and the illuminating light source 1 forms a collimated ray under the action of the concentrating array and projects on the light-transmitting object to be tested 2 to increase the output intensity of the optical signal.
  • a lens 12 is disposed on the optical path between the light-emitting device 2 and the first beam splitting mechanism, the second beam splitting mechanism, and the filter mechanism 3, and the lens 12 realizes transmission in the enhanced system by collecting light.
  • the workflow of the present invention is as follows, the illumination light source 1, the concentrating array 11, the filter wheel 31, the light-to-be-tested object 2, and the plurality of beam splitters 4 are sequentially disposed on a straight path of light propagation, and
  • the corresponding narrowband filter 41 and optical signal receiver 43 constitute a first spectroscopic detection system; the other dichroic mirror 4 and the corresponding optical signal receiver 43, fluorescent filter 42 and fluorescent signal receiver 44 are set in the light to be tested
  • the light path on the side of the object 2 constitutes a second spectroscopic detection system; the white light emitted by the illumination source 1 is concentrated by the concentrating array 11 to form a collimated ray, and the filter reel 31 is screened to output a monochromatic spot.
  • the light passes through the object to be tested 2, and the light generates a transmitted light signal and a scattered light signal on the light-transmitting object to be tested 2; wherein the transmitted light signal sequentially passes through the plurality of beam splitters 4 in the first beam splitting mechanism, and the reflected portion of the beam splitter 4 transmits partially
  • the optical signal is supplied to the corresponding narrow band filter 41.
  • the narrow band filter 41 allows only the optical signal of a specific wavelength to pass through and is obtained by the optical signal receiver 43, and the remaining transmitted optical signal continues to penetrate the next beam splitter 4 to repeat the above.
  • the scattered light signal generated by the optical communication test object 2 projected on the second beam means beam splitter 4, beam splitter reflecting portion 4 Powder The light signal is projected onto the fluorescent filter 42, excited to generate a fluorescent signal and obtained by the subsequent fluorescent signal receiver 44, and the remaining scattered light signal is transmitted through the beam splitter 4 by the subsequent signal receiver 43;
  • the optical signal input end and the output end of the light-transmitting object 2 are respectively provided with a lens 12, thereby increasing the input and output intensity of the optical signal.

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Abstract

一种检测仪光学系统，属于生化分析检测技术领域，包括照射光源（1）、光通待测物（2）、滤光机构（3）和探测器，滤光机构（3）设置在照射光源（1）和光通待测物（2）之间的入射光路上，光通待测物（2）的透射光路上设有第一分光机构、多组不同波长的窄带滤光片（41）和光信号接收器（43）；与光通待测物（2）透射光路垂直的光路上设有第二分光机构、光信号接收器（43）和荧光接收器（44）；照射光源（1）通过透镜（12）组成的聚光阵列（11）产生的准直光线在滤光机构（3）的筛选后进入光通待测物（2），光信号分别在不同波长的窄带滤光片（41）和荧光滤光片（42）的单色过滤后由探测器接收，从而实现一套系统的多重检测组合，整体结构紧凑合理、成本不高、使用灵活，可满足目前大部分光学检测试验的需求。

Description

一种检测仪光学系统 技术领域
[0001] 本发明涉及生化分析检测技术领域， 具体指一种检测仪光学系统。
背景技术
[0002] 生化检测原理有比色、 比浊和激发荧光的方法， 为了检测更多项目需要用到很 多波长， 这就需要将复色光分成很多的单色光。 目前常用的分光方式有以下几 种： 1.旋转滤光片轮分光方案， 一般用于前分光， 将不同波长的滤光片圆周阵列 放置于安装轮内， 需要用到哪个波长检测时就将旋转轮转到对应波长， 这种方 案简单且易于实现， 但效率较低、 设备体积较大； 2.分光镜阵列分光方案， 一般 用于后分光方式， 将经反应杯的复色光分成多个通道， 每个通道放置对应波长 的滤光片进行单色； 这种方案没有传动机构， 其可靠性高， 结构相对紧凑； 3.光 栅分光方案， 一般用于后分光方案， 光栅分光一次性可以分成很多波长， 结构 紧凑且效率高， 但也有成本高、 定位要求高、 杂散光较高的缺点。
[0003] 5见有生化检测仪器可以根据分析需求实现比色检测和透射比浊检测， 无法实现 散射比浊检测， 但透射比浊信噪比和灵敏度没有散射比浊高， 而且生化仪器也 不支持激发荧光检测， 功能较少， 不能满足多项目分析和高精确度的应用需求 ， 因此， 5见有技术还有待于改进和发展。
发明概述
技术问题
问题的解决方案
技术解决方案
[0004] 本发明的目的在于针对现有技术的缺陷和不足， 提供一种结构合理、 可以实现 透射、 散射和激发荧光的三种光学检测， 可满足多种对象的浓度检测要求的检 测仪光学系统。
[0005] 为了实现上述目的， 本发明采用以下技术方案：
[0006] 本发明所述的一种检测仪光学系统，包括照射光源、 光通待测物、 滤光机构和探 测器， 滤光机构设置在照射光源和光通待测物之间的入射光路上， 所述光通待 测物的透射光路上设有第一分光机构和对应的探测器， 探测器包括多组窄带滤 光片和光信号接收器； 所述光通待测物的折射光路上设有第二分光机构和对应 探测器， 探测器包括光信号接收器和荧光接收器。
[0007] 根据以上方案， 所述滤光机构包括滤光轮盘， 照射光源和滤光轮盘依次排列设 置在光通待测物的入射光路上， 所述滤光轮盘上设有出射通孔和若干单色滤光 片， 出射通孔和若干单色滤光片分布在同一圆周轨迹上， 且该转动圆周轨迹与 光通待测物的入射光路相交从而使出射通孔和若干单色滤光片可分别与照射光 源配对设置。
[0008] 根据以上方案， 所述第一分光机构包括若干分光镜， 若干分光镜依次间隔地在 光通待测物的透射光路上排列成分光阵列； 所述任一分光镜的反射光路上均设 有窄带滤光片和对应的光信号接收器， 若干窄带滤光片分别对应不同的单色波 长范围以过滤分光镜反射的光信号。
[0009] 根据以上方案， 所述分光阵列中相邻两个分光镜的反射光路方向相反。
[0010] 根据以上方案， 所述第二分光机构包括分光镜和変光滤光片， 分光镜和光信号 接收器依沿光通待测物的折射光路设置， 荧光接收器设于分光镜的反射光路上 ， 且荧光滤光片设于所述分光镜和荧光接收器之间。
[0011] 根据以上方案， 所述分光镜可以是二向色镜或中性分束镜中的任意一种。
[0012] 根据以上方案， 所述光路采用光纤作为光传播介质。
[0013] 根据以上方案， 所述照射光源和滤光机构之间的入射光路上设有聚光阵列， 聚 光阵列由若干依次排列的透镜组成， 照射光源在聚光阵列的作用下形成准直光 线并投射于光通待测物上。
[0014] 根据以上方案， 所述光通待测物与第一分光机构、 第二分光机构、 滤光机构之 间的光路上均设有透镜。
发明的有益效果
有益效果
[0015] 本发明有益效果为： 本发明结构合理， 照射光源通过透镜组成的聚光阵列产生 的准直光线在滤光机构的筛选后进入光通待测物， 产生的透射、 散射光信号分 别经由第一和第二分光机构传导， 光信号分别在不同波长的窄带滤光片和荧光 滤光片的单色过滤后由探测器接收， 从而实现一套系统的多重检测组合， 整体 结构紧凑、 成本不高、 使用灵活， 可满足目前大部分光学检测试验的需求。 对附图的简要说明
附图说明
[0016] 图 1是本发明的整体结构示意图；
[0017] 图 2是本发明的滤光机构部分结构示意图。
[0018] 图中：
[0019] 1、 照射光源； 2、 光通待测物； 3、 滤光机构； 4、 分光镜； 5、 光纤； 11、 聚 光阵列； 12、 透镜； 31、 滤光轮盘； 32、 出射通孔； 33、 单色滤光片； 41、 窄 带滤光片； 42、 荧光滤光片； 43、 光信号接收器； 44、 荧光接收器。
实施该发明的最佳实施例
本发明的最佳实施方式
[0020] 下面结合附图与实施例对本发明的技术方案进行说明。
[0021] 如图 1所示， 一种检测仪光学系统，包括照射光源 1、 光通待测物 2、 滤光机构 3和 探测器， 滤光机构 3设置在照射光源 1和光通待测物 2之间的入射光路上， 所述光 通待测物 2的透射光路上设有第一分光机构和对应的探测器， 探测器包括多组窄 带滤光片 41和光信号接收器 43 ; 所述光通待测物 2的折射光路上设有第二分光机 构和对应探测器， 探测器包括光信号接收器 43和荧光接收器 44; 所述照射光源 1 产生光线进入光通待测物 2发生透射和散射现象， 其中透射光线沿光路穿过第一 分光机构， 第一分光机构依次反射不同波长的光信号给对应的探测器， 通过多 个光信号接收器 43接收对应的信息以实现比色检测， 而散射光线沿光路穿过第 二分光机构， 对应的散射光信号接收器 43和荧光接收器 44用于实现比浊和激发 荧光项目检测。
[0022] 上述入射光路和透射光路处于穿过光通待测物 2的同一直线上， 从而使照射光 源 1发出的光线沿入射光路投射于光通待测物 2上， 光线在光通待测物 2上产生透 射光信号和散射光信号， 其中透射光信号沿透射光路继续传播， 而散射光信号 沿折射光路传播， 该折射光路泛指除了光通待测物 2的入射光路和透射光路以外 的任意散射方向， 本案中所述的折射光路与光通待测物 2的透射光路垂直以获得 最强的散射光信号和最佳的结构布局， 则该折射光路可以是以透射光路为轴心 的任意径向。
[0023] 如图 2所示， 所述滤光机构 3包括滤光轮盘 31， 照射光源 1和滤光轮盘 31依次排 列设置在光通待测物 2的入射光路上， 所述滤光轮盘 31上设有出射通孔 32和若干 单色滤光片 33 , 出射通孔 32和若干单色滤光片 33分布在同一圆周轨迹上， 且该 转动圆周轨迹与光通待测物 2的入射光路相交从而使出射通孔 32和若干单色滤光 片 33可分别与照射光源 1配对设置； 所述滤光机构 3用于根据检测项目过滤照射 光源 1的光谱范围， 出射通孔 32则对应全波长光线以进行比色检测， 当对应的单 色滤光片 33随滤光轮盘 31转动到入射光路时， 仅允许单色滤光片 33波长范围内 的单色光线能穿过并经由入射光路照射在光通待测物 2, 从而过滤多余光信号以 降低对检测结果的干扰。
[0024] 所述第一分光机构包括若干分光镜 4， 若干分光镜 4依次间隔地在光通待测物 2 的透射光路上排列成分光阵列， 若干分光镜 4均与光通待测物 2的透射光路呈 45° 夹角设置； 所述任一分光镜 4的反射光路上均设有窄带滤光片 41和对应的光信号 接收器 43 , 若干窄带滤光片 41分别对应不同的单色波长范围以过滤分光镜 4反射 的光信号； 从光通待测物 2中穿透的光线依次经过分光阵列中的若干分光镜 4， 分光镜 4反射并在窄带滤光片 41的过滤后输出给光信号接收器 43 , 而剩余的其他 波长光线继续穿透下一个分光镜 4并反射对应光信号， 本方案的分光镜 4数量可 根据检测需求进行增加和缩减， 但分光阵列中应当至少包含了 340、 405、 505、 546、 578、 630、 660、 700这些常用波长的分光镜 4和窄带滤光片 41。
[0025] 所述分光镜 4可以是二向色镜或中性分束镜中的任意一种， 二向色镜是指仅反 射特定波长光线的分光镜 4, 当需要波长种类较多时采用二向色镜， 可以提高长 波波长的信号值； 中性分束镜可在分光阵列数量较小时使用， 从而在特定范围 检测时减少元器件种类以缩减结构成本。
[0026] 所述分光阵列中相邻两个分光镜 4的反射光路方向相反， 在如图 1所示的分光阵 列布局中， 相邻的两个分光镜 4之间呈 90°夹角设置， 第一分光机构两侧的探测器 对称布局， 可有效地缩减和紧凑检测仪整体结构， 提高光信号传递效率。 [0027] 所述第二分光机构包括分光镜 4和変光滤光片 42， 分光镜 4和光信号接收器 43依 沿光通待测物 2的折射光路设置， 荧光接收器 44设于分光镜 4的反射光路上， 且 荧光滤光片 42设于所述分光镜 4和荧光接收器 44之间， 从光通待测物 2中散射的 光线进入第二分光机构， 第二分光机构用于散射光比浊检测和激发荧光检测， 散射光线穿透分光镜 4后由对应的光信号接收器 43接收， 而经分光镜 4反射的光 线经过荧光滤光片 42截止激发光输出给荧光接收器 44。
[0028] 所述光路可采用光纤 5作为光传播介质， 所述光路包括了照射光源 1与光通待测 物 2之间的入射光路、 光通待测物 2与第一分光机构之间的透射光路、 光通待测 物 2与第二分光机构之间的折射光路； 所述光纤 5用于搭建本系统的光路以连接 各主体部件， 起到隔离热源、 维持光线传播方向和降低信号损失的作用。
[0029] 所述照射光源 1可采用南素灯或 LED光源， 照射光源 1和滤光机构 3之间的入射 光路上设有聚光阵列， 聚光阵列由若干依次排列的透镜 12组成， 透镜 12通过聚 焦作用对光线进行汇聚和校准， 照射光源 1在聚光阵列的作用下形成准直光线并 投射于光通待测物 2上从而提高光信号的输出强度。
[0030] 所述光通待测物 2与第一分光机构、 第二分光机构、 滤光机构 3之间的光路上均 设有透镜 12, 所述透镜 12通过汇聚光线实现增强系统中传递的光信号强度的目 的。
[0031] 本发明的工作流程如下， 所述照射光源 1、 聚光阵列 11、 滤光轮盘 31、 光通待 测物 2和若干分光镜 4依次设置在光传播的直线路径上， 与若干对应的窄带滤光 片 41和光信号接收器 43组成第一分光检测系统； 另一个分光镜 4与对应的光信号 接收器 43、 荧光滤光片 42和荧光信号接收器 44设置在光通待测物 2—侧的光路上 组成第二分光检测系统； 所述照射光源 1发出的白色光通过聚光阵列 11汇聚形成 准直光线， 滤光轮盘 31对其进行筛选以输出单色光斑照射于光通待测物 2上， 光 线在光通待测物 2上产生透射光信号和散射光信号； 其中的透射光信号依次经过 第一分光机构中的若干分光镜 4, 分光镜 4反射部分透射光信号给对应的窄带滤 光片 41， 窄带滤光片 41仅允许特定波长的光信号穿过并由光信号接收器 43获得 ， 而剩余的透射光信号继续穿透下一个分光镜 4重复上述步骤； 相应的， 光通待 测物 2产生的散射光信号投射于第二分光机构中的分光镜 4， 分光镜 4反射部分散 射光信号并投射于荧光滤光片 42上， 激发产生荧光信号并由其后的荧光信号接 收器 44获得， 而剩余散射光信号穿透分光镜 4由其后的信号接收器 43获得； 本发 明优选的光通待测物 2的光信号入射端、 出射端分别设有透镜 12, 从而提高光信 号的输入输出强度。
[0032] 以上所述仅是本发明的较佳实施方式， 故凡依本发明专利申请范围所述的构造 、 特征及原理所做的等效变化或修饰， 均包括于本发明专利申请范围内。

Claims

权利要求书
[权利要求 1] 一种检测仪光学系统，包括照射光源 （1） 、 光通待测物 （2） 、 滤光 机构 （3） 和探测器， 滤光机构 （3） 设置在照射光源 （1） 和光通待 测物 （2） 之间的入射光路上， 其特征在于： 所述光通待测物 （2） 的 透射光路上设有第一分光机构和对应的探测器， 探测器包括多组窄带 滤光片 （41） 和光信号接收器 （43） ; 所述光通待测物 （2） 的折射 光路上设有第二分光机构和对应探测器， 探测器包括光信号接收器 （ 43） 和荧光接收器 （44） 。
[权利要求 2] 根据权利要求 1所述的检测仪光学系统， 其特征在于： 所述滤光机构 （3） 包括滤光轮盘 （31） ， 照射光源 （1） 和滤光轮盘 （31） 依次排 列设置在光通待测物 （2） 的入射光路上， 所述滤光轮盘 （31） 上设 有出射通孔 （32） 和若干单色滤光片 （33） ， 出射通孔 （32） 和若干 单色滤光片 （33） 分布在同一圆周轨迹上， 且该转动圆周轨迹与光通 待测物 （2） 的入射光路相交从而使出射通孔 （32） 和若干单色滤光 片 （33） 可分别与照射光源 （1） 配对设置。
[权利要求 3] 根据权利要求 1所述的检测仪光学系统， 其特征在于： 所述第一分光 机构包括若干分光镜 （4） ， 若干分光镜 （4） 依次间隔地在光通待测 物 （2） 的透射光路上排列成分光阵列； 所述任一分光镜 （4） 的反射 光路上均设有窄带滤光片 （41） 和对应的光信号接收器 （43） ， 若干 窄带滤光片 （41） 分别对应不同的单色波长范围以过滤分光镜 （4） 反射的光信号。
[权利要求 4] 根据权利要求 3所述的检测仪光学系统， 其特征在于： 所述分光阵列 中相邻两个分光镜 （4） 的反射光路方向相反。
[权利要求 5] 根据权利要求 1所述的检测仪光学系统， 其特征在于： 所述第二分光 机构包括分光镜 （4） 和荧光滤光片 （42） ， 分光镜 （4） 和光信号接 收器 （43） 依沿光通待测物 （2） 的折射光路设置， 荧光接收器 （44 ） 设于分光镜 （4） 的反射光路上， 且荧光滤光片 （42） 设于所述分 光镜 （4） 和荧光接收器 （44） 之间。
[权利要求 6] 根据权利要求 3-5任一所述的检测仪光学系统， 其特征在于： 所述分 光镜 （4） 可以是二向色镜或中性分束镜中的任意一种。
[权利要求 7] 根据权利要求 1-5任一所述的检测仪光学系统， 其特征在于： 所述光 路采用光纤 （5） 作为光传播介质。
[权利要求 8] 根据权利要求 1所述的检测仪光学系统， 其特征在于： 所述照射光源 （1） 和滤光机构 （3） 之间的入射光路上设有聚光阵列， 聚光阵列由 若干依次排列的透镜 （12） 组成， 照射光源 （1） 在聚光阵列的作用 下形成准直光线并投射于光通待测物 （2） 上。
[权利要求 9] 根据权利要求 1所述的检测仪光学系统， 其特征在于： 所述光通待测 物 （2） 与第一分光机构、 第二分光机构、 滤光机构 （3） 之间的光路 上均设有透镜 （12） 。
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Publication number Priority date Publication date Assignee Title
CN108776103A (zh) * 2018-05-15 2018-11-09 三诺生物传感股份有限公司 一种检测仪光学系统
CN109357982B (zh) * 2018-11-13 2024-02-09 重庆川仪分析仪器有限公司 粉尘仪自动校准装置
CN112748126A (zh) * 2019-10-31 2021-05-04 芯恩（青岛）集成电路有限公司 晶圆检测系统及检测方法
CN111208108A (zh) * 2020-02-27 2020-05-29 光越科技(深圳)有限公司 一种荧光探测方法、系统及装置
CN112255207B (zh) * 2020-09-22 2023-12-12 广州源起健康科技有限公司 一种荧光激发采集系统
CN113358570A (zh) * 2021-06-11 2021-09-07 宋卓 分光结构及包含该结构的微粒检测装置及其检测方法
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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4648714A (en) * 1985-09-11 1987-03-10 University Of Utah Molecular gas analysis by Raman scattering in intracavity laser configuration
JP2003014646A (ja) * 2001-06-27 2003-01-15 Nikon Corp 蛍光測定装置
CN2651746Y (zh) * 2003-09-15 2004-10-27 上海欧陆科仪有限公司 旋转式浊度测量装置
CN1815196A (zh) * 2005-02-03 2006-08-09 三星电子株式会社 多通道荧光测量光学系统及多通道荧光样本分析仪
CN1914502A (zh) * 2004-01-30 2007-02-14 纳尔科公司 可更换探头－开放式样品池荧光计
CN102012368A (zh) * 2010-10-09 2011-04-13 中国科学院上海光学精密机械研究所 上转换发光颗粒多参数检测系统和方法
CN108776103A (zh) * 2018-05-15 2018-11-09 三诺生物传感股份有限公司 一种检测仪光学系统
CN208255041U (zh) * 2018-05-15 2018-12-18 三诺生物传感股份有限公司 一种检测仪光学系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006258776A (ja) * 2004-04-08 2006-09-28 Nippon Koden Corp 粒子分類装置
CN204389386U (zh) * 2015-01-05 2015-06-10 任峰 一种多光谱透射、散射比浊法和化学发光反射法检测用检测头及检测浊度仪
CN104730054B (zh) * 2015-04-10 2018-08-21 中国科学院烟台海岸带研究所 一种一体化探头式光电水质多参数在线测量系统
CN105300885A (zh) * 2015-12-02 2016-02-03 南京华欣分析仪器制造有限公司 分析仪器的光路装置

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4648714A (en) * 1985-09-11 1987-03-10 University Of Utah Molecular gas analysis by Raman scattering in intracavity laser configuration
JP2003014646A (ja) * 2001-06-27 2003-01-15 Nikon Corp 蛍光測定装置
CN2651746Y (zh) * 2003-09-15 2004-10-27 上海欧陆科仪有限公司 旋转式浊度测量装置
CN1914502A (zh) * 2004-01-30 2007-02-14 纳尔科公司 可更换探头－开放式样品池荧光计
CN1815196A (zh) * 2005-02-03 2006-08-09 三星电子株式会社 多通道荧光测量光学系统及多通道荧光样本分析仪
CN102012368A (zh) * 2010-10-09 2011-04-13 中国科学院上海光学精密机械研究所 上转换发光颗粒多参数检测系统和方法
CN108776103A (zh) * 2018-05-15 2018-11-09 三诺生物传感股份有限公司 一种检测仪光学系统
CN208255041U (zh) * 2018-05-15 2018-12-18 三诺生物传感股份有限公司 一种检测仪光学系统

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