WO2019124854A1 - Composition for preventing or treating dry eye syndrome, containing mixed extract or fraction thereof - Google Patents
Composition for preventing or treating dry eye syndrome, containing mixed extract or fraction thereof Download PDFInfo
- Publication number
- WO2019124854A1 WO2019124854A1 PCT/KR2018/015687 KR2018015687W WO2019124854A1 WO 2019124854 A1 WO2019124854 A1 WO 2019124854A1 KR 2018015687 W KR2018015687 W KR 2018015687W WO 2019124854 A1 WO2019124854 A1 WO 2019124854A1
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- WIPO (PCT)
- Prior art keywords
- fraction
- dry eye
- eye syndrome
- licorice
- extract
- Prior art date
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- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000002511 suppository base Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
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- 235000013616 tea Nutrition 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000011200 topical administration Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
Definitions
- the present invention relates to a pharmaceutical composition for preventing or treating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient, and a health functional food for preventing or ameliorating dry eye syndrome comprising the mixed extract or the fraction thereof as an active ingredient .
- Dry eye syndrome is not simply a tear defect, but it causes eye discomfort, eye fatigue, decreased visual acuity, and instability of the tear layer due to inflammation of the tear and ocular surface (cornea and conjunctiva) .
- inflammation plays an important role in infiltration of inflammatory cells, increased expression of immune activation molecules and adhesion molecules, Th1 and Th17 responses, abnormalities of apoptotic markers and chemokines have.
- dry eye syndrome is a common disease that occurs in about 20% of adults in Korea. Especially, global warming due to global climate change, especially El Ni ⁇ o phenomenon, and environmental pollution such as fine dust, There is no therapeutic or functional food.
- Paeonia lactiflora is a perennial plant belonging to the family Paeoniaceae and is native to parts of China, Siberia and Korea, and the herbal medicine and herbal medicines are defined as the roots of Paeonia lactiflora Pallas or other herbaceous plants .
- the pharmacological activities of peonies have been reported to inhibit platelet aggregation, to act on the circulatory system, to antitumor action, and to enhance immunity (Son Dong-Ju, Journal of the Korean Society of Food Science and Nutrition, 2002, 31, 511, 4, 445).
- Licorice ( Glycyrrhiza uralensis ) is a perennial herbaceous plant belonging to the soybean family.
- the pharmacological activity of licorice was evaluated by the antitumor effect of chronic hepatitis improvement, antidiabetic effect (Mae, Tatsumasa, et al., The Journal of nutrition 133.11 (2003): 3369-3377) , Korean Journal of Plant Resources 27.5 (2014): 401-414.), Ulcerative Colitis Inhibitory Effect (Lee, Keyong-Ho, and Ki-Hyeong Rhee., The Korean Journal of Food and Nutrition 23.4 -439.), Antioxidant effects (Woo, Koan-Sik, et al., Journal of the Korean Society of Food Science and Nutrition 36.6 (2007): 689-695).
- the present inventors have completed the present invention by confirming that a mixed extract of peony root and licorice, or a fraction thereof, can treat dry eye syndrome through an increase in tear secretion amount and inhibition of corneal morphological change.
- Another object of the present invention is to provide a composition for preventing or treating dry eye syndrome comprising a mixture of peony root and licorice root extract or a fraction thereof as an active ingredient.
- Another object of the present invention is to provide a health functional food for preventing or ameliorating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- It is another object of the present invention to provide a feed composition for preventing or improving dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- Another object of the present invention is to provide a quasi-drug composition for the prevention or treatment of dry eye syndrome comprising a mixture of peony root and licorice root extract or a fraction thereof as an active ingredient.
- Another object of the present invention is to provide an eye cosmetic additive composition for preventing or improving dry eye syndrome, which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- Another object of the present invention is to provide a method for preventing or treating dry eye syndrome comprising administering to a subject a mixed extract of licorice and licorice or a fraction thereof.
- the peanut and licorice mixed extract of the present invention and the fractions thereof can be used for the development of a pharmaceutical composition for the prevention or treatment of dry eye syndrome since the amount of tear secretion due to dry eye syndrome and the change of corneal morphology can be significantly inhibited It will be possible.
- FIG. 1 is a photograph showing a change in the amount of tear secretion according to the administration of the mixed extract through an eye volume test sheet.
- FIG. 2 is a graph showing a quantitative change in the amount of tear secretion according to the mixed extract administration (*: p ⁇ 0.05 vs. Normal; #: p ⁇ 0.05 vs. dry eye).
- FIG. 3 is a photograph showing changes in the corneal morphology of rats upon administration of mixed extracts.
- FIG. 4 is a graph showing scores of corneal smoothness score in rats according to the mixed extract administration.
- the corneal smoothness score was evaluated by dividing the corneal morphology by 0 to 5 (zero point: no distortion, 1 point: distortion at 1/4 point, 2 points: 2/4 point , 3 points: Distortion at 3/4 point, 4 points: Distortion at all points, 5 points: Uncertainty of rounded shape due to severe distortion, #: p ⁇ 0.05 vs. dry eye).
- FIG. 5 is a graph showing a quantitative change in the amount of tear secretion according to administration of licorice extract (*: p ⁇ 0.05 vs. Normal).
- FIG. 6 is a photograph showing changes in the corneal morphology of rats upon administration of licorice extract.
- FIG. 7 is a graph showing the corneal smoothness score of rats according to administration of mixed extract (*: p ⁇ 0.05 vs. Normal).
- the inventors of the present invention have found that when a variety of studies are conducted to develop a therapeutic agent capable of effectively treating dry eye syndrome, a mixed extract of peony root and licorice or a fraction thereof shows an effect of treating dry eye syndrome.
- the above-mentioned mixed extracts or fractions of peanut and licorice not only significantly inhibited the decrease of tear secretion and changes in corneal morphology according to dry eye syndrome but also showed similar effects to the commercial drugs used for the treatment of dry eye syndrome there was.
- the technique for treating dry eye syndrome using the mixed extract or fraction has not been known at all and has been developed for the first time by the present inventors.
- the present invention provides a pharmaceutical composition for preventing or treating dry eye syndrome, which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- the present invention also provides a composition for preventing, ameliorating or treating dry eye syndrome, comprising a mixture of peony root and licorice, or a fraction thereof as an active ingredient.
- the mixed extract of peanut and licorice having the preventive and therapeutic activity of dry eye syndrome can be extracted from various organs of natural, hybrid, and variant plants and can be extracted from various organs such as roots, stems, leaves, flowers, As well as extracts from plant tissue cultures.
- Paeonia lactiflora is a perennial herb that belongs to the family Paeoniaceae and is native to parts of China, Siberia and Korea.
- peonies are defined as the roots of Paeonia lactiflora or other plants, and peeled, dried and dried together with peel and peel are called peptone.
- the pharmacological activities of peonies have been reported to inhibit platelet aggregation, to act on the circulatory system, to antitumor action, and to enhance immunity.
- Glycyrrhiza uralensis is a perennial herbaceous plant belonging to the soybean family and may be used as a medicinal or sweetener by drying the roots.
- the pharmacological activity of licorice has been reported to improve chronic hepatitis by detoxification, anti - diabetic effect, anti - ulcer effect, ulcerative colitis inhibitory effect, antioxidant effect.
- the mixed extract may be extracted from roots, stems, leaves, flowers, or fruits of peony root and licorice, and is not particularly limited thereto.
- the mixed extract of peony root and licorice includes, but is not limited to, extracts from a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof.
- the above extract may contain any one of the extract obtained by the extraction treatment, the diluted solution or the concentrate of the extract, the dried product obtained by drying the extract, or the adjusted product or the purified product.
- the alcohol may be butyl alcohol, ethyl alcohol, methyl alcohol or a water-soluble alcohol thereof.
- the water-soluble alcohol may be an alcohol in an amount of 0.1 to 100% by weight.
- fraction means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
- the fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art.
- a method of obtaining a fraction from the extract by treating a predetermined solvent with an extract obtained by extracting a mixed extract of peony root and licorice root is not particularly limited and may be carried out according to a method commonly used in the art.
- the fraction may be a hexane fraction, an ethyl acetate fraction, a butanol fraction or a water fraction of a mixture of peony root and licorice.
- the fractions may each comprise 0.01 to 100% by weight, more specifically 1 to 80% by weight, based on the total weight of the pharmaceutical composition.
- the kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used.
- Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more.
- polar solvents such as water and alcohol
- non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more.
- C1 to C4 alcohols can be specifically used.
- dry eye syndrome refers to dry eye syndrome, which is caused by inflammation of the lacrimal gland and decongestion of the cornea, inhibiting tear secretion, And abnormal conjunctival epithelial disorder caused by excessive dryness of the tears due to the dry eye syndrome and resulting in wounds of the cornea.
- prevention refers to any act that inhibits or delays the symptoms of dry eye syndrome by administration of a composition comprising the peony root extract of licorice and licorice, or fractions thereof, according to the present invention.
- treatment refers to any action that improves or alleviates the symptoms of dry eye syndrome by administration of a composition comprising the peony root extract and licorice root extract or fractions thereof according to the present invention.
- the composition may increase the secretion amount of tears or inhibit the morphological change of the cornea.
- the morphological change of the cornea may be due to excessive irritation of the tears, which may cause the surface of the cornea to become rugged and the rounded circle to warp and distort.
- the composition of the present invention may improve smoothness and flatness.
- the tear secretion amount was significantly increased in all the concentration groups when the mixed extract of peony root and Licorice was administered, and it was confirmed that the effect was increased depending on the concentration (FIGS. 1 and 2 ).
- the term "pharmaceutical composition” means a preparation intended for the prevention or treatment of disease, and may be formulated into various forms according to each ordinary method.
- it can be formulated into a form such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and the like, and can be formulated in the form of external preparations, suppositories and sterilized injection solutions.
- it can be formulated into an external preparation for eye, a form suitable for topical administration, for example, an eye drop, a cream, an ointment, a gel or a lotion.
- composition of the present invention may be prepared in the form of a pharmaceutical composition for preventing or treating dry eye syndrome, which further comprises an appropriate carrier, excipient or diluent conventionally used in the production of a pharmaceutical composition, (non-naturally occuring carrier).
- the carrier, excipient and diluent which may be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.
- a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.
- Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like.
- lubricants such as magnesium stearate and talc are also used.
- Liquid preparations for oral use may include various excipients such as wetting agents, sweetening agents, fragrances, preservatives, etc.
- Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories.
- the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.
- the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
- the dosage of the pharmaceutical composition of the present invention can be determined by those skilled in the art in consideration of the purpose of use, the degree of addiction to the disease, the age, body weight, sex, history, or kind of the substance used as the active ingredient.
- the pharmaceutical composition of the present invention may be administered at a dose of from about 0.1 ng to about 1,000 mg / kg, in particular from 1 ng to 1,000 mg / kg, per adult, and the frequency of administration of the composition of the present invention is specifically limited However, it may be administered once a day or divided into several doses. The dose is not intended to limit the scope of the invention in any way.
- the present invention provides a method for treating dry eye syndrome comprising administering the pharmaceutical composition to a subject suffering from dry eye syndrome in a pharmaceutically effective amount.
- the term "individual” as used herein may include, without limitation, mammals including rats, livestock, and the like with dry eye syndrome.
- the "individual” may include companion animals.
- the companion animal refers to an animal living together with a human, and specific examples include, but are not limited to, mammals such as dogs, cats, hamsters, and guinea pigs, and birds such as parrots and canaries.
- composition may be administered in single or multiple doses in a pharmaceutically effective amount.
- the administration route of the pharmaceutical composition for preventing or treating dry eye syndrome of the present invention can be administered through any ordinary route as long as it can reach the target tissue.
- composition of the present invention may be administered orally, intraperitoneally, intramuscularly, subcutaneously, intradermally, transdermal patch, oral, intranasal, intrapulmonary, Intramuscular administration, intrathecal administration, intrarectal administration, and the like, and specifically administered orally.
- the present invention provides a health functional food for preventing or ameliorating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- peanut, licorice, fraction, and dry eye syndrome in the health functional food of the present invention is as defined above.
- the term “improvement” means any action that alters or modifies dryness of the eye by orally administering the composition.
- the term "functional" as used in the present invention means that the structure and function of the human body have a beneficial effect for health use such as controlling nutrients or physiological action.
- the health functional food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients that are conventionally added in the art.
- the formulation of the health functional food may also be produced without limitation as long as the formulation is recognized as a health functional food.
- the health functional food of the present invention can be manufactured in various forms, and unlike general pharmaceuticals, it has advantages of being free from side effects that may occur when a food is used as a raw material for a long period of time, and is excellent in portability, Can be ingested as an adjuvant to improve the effect of preventing or improving dry eye syndrome.
- the health functional food is a food prepared by adding the mixed extract or fraction of the present invention to a food material such as beverage, tea, spice, gum, confectionery, or the like, or encapsulating, powdering, or suspending, But it has the advantage that there is no side effect that can occur when a drug is taken for a long time using a food as a raw material, unlike a general medicine.
- an eye cosmetic additive composition for preventing or improving dry eye syndrome comprising extracts of Euphorbiaceae or fractions thereof as an active ingredient.
- the definition of the extract of Eisenhower extract, fraction, dry eye syndrome, prevention and improvement in the eye cosmetic additive composition is as defined above.
- the additive may be included in eye make-up products and may be effective in preventing or improving the disease.
- the composition may include eye make-up products.
- eye make-up cosmetic products include any one selected from the group consisting of eye pencil, eye liner, eye shadow, mascara, and eye makeup remover. have.
- a quasi-drug composition for preventing or improving dry eye syndrome comprising extracts of Euphorbiaceae or fractions thereof as an active ingredient.
- Quasi-drug product in the present invention means products which are less active than drugs, for example, for the purpose of diagnosing, treating, improving, alleviating, treating or preventing a disease in a human or an animal.
- Quasi-drugs are products that are not used for medicines, such as fibers and rubber products used for the treatment and prevention of diseases of humans and animals, and are not acting directly or indirectly on the human body, These include sterilization and insecticides to prevent infectious diseases.
- the type and formulations of the quasi-drug composition of the present invention are not particularly limited, but may be disinfectant cleaner, shower foam, gagrin, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment or filter filler.
- a feed composition for preventing or ameliorating dry eye syndrome comprising extracts of Euphorbiaceae or fractions thereof as an active ingredient.
- feed means any natural or artificial diet, single meal or the like or ingredients of the above formula for ingesting, ingesting, digesting or suitable for the individual.
- the kind of the feed is not particularly limited, and feeds conventionally used in the art can be used.
- feeds include vegetable feeds such as cereals, muscle roots, food processing busines logistics, algae, fibers, pharmaceuticals, buses, oils, pastes, pulses or cereals; Animal feeds such as proteins, inorganic substances, fats, oils, fats, oils, monocellular proteins, animal plankton or foods. These may be used alone or in combination of two or more.
- the tear secretion amount was significantly increased in all the concentration groups when the mixed extract of peony root and Licorice was administered, and it was confirmed that the effect was increased depending on the concentration (FIGS. 1 and 2 ).
- the above-mentioned results indicate that the mixed extract of peony root and licorice or its fractions can be used as a pharmaceutical composition for preventing dry eye disease or eye fatigue, for improving or ameliorating eye fatigue, a health functional food, an eye cosmetic additive, And it can be used effectively.
- the extracts were filtered, and the filtrate was concentrated under reduced pressure at about 50 ° C and dried to prepare a mixed extract.
- the experimental group consisted of the following: non-invasive normal group (NOR) with no abnormality, the disease caused by dry eye lacrimal gland by the method of Example 2.1 (JGT-50) administered with 50 mg / kg of the mixed extract of the present invention (JGT), a drug administration group (JGT-50) administered with 100 mg / kg of the mixed extract of the present invention (JGT- 100), the drug-administered group (JGT-250) in which 250 mg / kg of the mixed extract of the present invention (JGT) was administered.
- the drug containing the mixed extract of the present invention was prepared by dissolving the above dose in sterilized physiological saline containing 0.5% DMSO.
- the dry eye was additionally administered on the 5th day for 3 days.
- the animals were anesthetized and the change in color of the test strip was measured by tearing after 30 seconds of contact with the phenol-red thread volume test (FCI Opthalmics Zone Quick, Japan) And the efficacy of the drug was evaluated.
- composition of the test group and administration of the drug were carried out in the same manner as in Example 2.2.
- the animals were anesthetized and photographed using a stereoscopic microsope (Olympus, Japan) equipped with a fiberoptic ring illuminator. Changes in corneal morphology were assessed by grading the degree of distortion of the rounded circle shape of the circular light reflected on the corneal epithelium from 0 to 5. 0 point: No distortion, 1 point: Distortion at 1/4 point, 2 points: Distortion at 2/4 point, 3 points: Distortion at 3/4 point, 4 points: Distortion at all points, 5 point: Distortion at severe point Can not recognize the shape of the round circle.
- the morphological changes of the cornea were evaluated.
- the tears secreted from the external lacrimal gland formed a tear film, and the smooth and flat surface state was maintained.
- the smooth and flat surface state was maintained.
- abnormalities in the tear film covering the cornea resulted in degeneration of the epithelium of the corneal epithelium and smooth surface of the cornea, so that the rounded shape was distorted and distorted.
- JGT-100 and JGT-250 significantly inhibited corneal morphological changes such as the disease group (FIGS. 3 and 4).
- the tear secretion amount was measured using the method of Example 2.2 in order to confirm the therapeutic effect of the licorice extract extracted by the method of Example 1 with dry liquorice alone.
- the dose of licorice extract was 84 mg / kg, the dose of Licorice based on JGT-250 (250 mg / kg dose).
- the tear secretion amount of the licorice extract-treated group was not significantly different from that of the dry eye syndrome group, confirming that the licorice extract did not increase the tear secretion amount.
- Example 2.3 In order to confirm the therapeutic effect of dry eye extract of licorice root extract, the change of corneal morphology was confirmed by the method of Example 2.3.
- the dose of licorice extract was 84 mg / kg, the dose of Licorice based on JGT-250 (250 mg / kg dose).
- the licorice extract alone did not affect the amount of tear secretion, and had no effect of inhibiting the corneal morphological change. Thus, it was confirmed that it was not suitable as a composition for treating dry eye syndrome.
Abstract
The present invention relates to a pharmaceutical composition for preventing or treating dry eye syndrome, containing, as an active ingredient, a mixed extract of Paeonia lactiflora and Glycyrrhiza uralensis or a fraction thereof, and a functional health food for preventing or alleviating dry eye syndrome, containing, as an active ingredient, the mixed extract or a fraction thereof. The mixed extract of Paeonia lactiflora and Glycyrrhiza uralensis or a fraction thereof, of the present invention, can significantly inhibit a reduction in tear secretion and topographic changes in the cornea, caused by dry eye syndrome, and thus can be provided as an active ingredient of a pharmaceutical composition and a functional health food for preventing or treating dry eye syndrome and in a treatment method.
Description
본 발명은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 치료용 약학 조성물 및 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 개선용 건강기능식품에 관한 것이다.The present invention relates to a pharmaceutical composition for preventing or treating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient, and a health functional food for preventing or ameliorating dry eye syndrome comprising the mixed extract or the fraction thereof as an active ingredient .
안구 건조증(건성안: dry eye syndrome)은 단순히 눈물부족이 아닌, 눈물과 안구표면(각막 및 결막)의 염증에 의한 안구의 불편감, 눈피로, 시력저하, 눈물층의 불안정성을 유발하여 안구표면에 손상을 주어, 통증, 불규칙한 각막 표면, 흐리고 변동폭이 커진 시력 및 각막궤양 등의 발병 위험성이 크다. 그러나 이러한 발병기전은 아직 완전히 규명되지 않았으나, 염증세포의 침윤, 면역활성화 분자 및 접착분자발현 증가, Th1 및 Th17 반응, 세포사멸 마커 및 케모카인의 비정상적인 변화 등 염증이 중요한 역할을 한다는 연구결과가 보고되고 있다. Dry eye syndrome is not simply a tear defect, but it causes eye discomfort, eye fatigue, decreased visual acuity, and instability of the tear layer due to inflammation of the tear and ocular surface (cornea and conjunctiva) , There is a great risk of pain, irregular corneal surface, blurred vision, increased corneal ulceration, and the like. However, the mechanisms of this pathogenesis have not yet been fully elucidated, but studies have shown that inflammation plays an important role in infiltration of inflammatory cells, increased expression of immune activation molecules and adhesion molecules, Th1 and Th17 responses, abnormalities of apoptotic markers and chemokines have.
일반적으로 안구 건조증 치료를 위해서 칼륨과 안토시아닌이 풍부한 음식섭취를 권장하고 있고, 이외에도 케일, 키위, 사과 등의 과일섭취도 적극 권장하고 있다, 또한 인공눈물 점안, 눈물점을 막아 배출되는 눈물의 양을 조절하는 치료법을 사용하기도 한다. In general, it is recommended to consume foods rich in potassium and anthocyanins for the treatment of dry eye syndrome. In addition, fruits such as kale, kiwi and apple are also highly recommended, and the amount of tears released by artificial tear drops and tear points They also use controlled treatments.
글로벌 데이터 보고서에 의하면 세계 안구건조증 시장은 2012년 16억 달러 (1조8816억원)에서 2011년에는 55억 달러(6조4680억원)로 연평균 12.8%의 성장률을 보일 것을 전망하였다. 건강보험심사평가원 자료에 의하면 국내 안구건조증 치료에 사용된 총 진료비는 연평균 8.6%씩 증가해 2009년에는 521억원, 2013년에는 726억원으로 5년 사이 200억원 이상 증가하였다(이투데이, 2016. 06.27).According to the Global Data Report, the world market for dry eye syndrome is expected to grow at a CAGR of 12.8% from US $ 1.6 billion in 2012 (US $ 1.88 trillion) to US $ 5.5 billion (US $ 6.4 trillion) in 2011. According to the Health Insurance Review and Assessment Service, total medical expenses used for the treatment of dry eye syndrome in Korea increased by 8.6% per year, reaching 52.1 billion won in 2009 and 72.6 billion won in 2013 (over 2 million won in 2016) .
그러나 안구건조증은 우리나라 성인의 20% 내외로 발생하는 흔한 질병으로, 특히 전 세계적인 기후 변화 특히 엘리뇨 현상으로 온도가 올라가고 있는 상황과 미세먼지 등 환경오염으로 인해 안구건조증 환자가 지속적으로 증가하고 있으나, 특별한 치료제 또는 기능성 식품이 없는 상황이다. However, dry eye syndrome is a common disease that occurs in about 20% of adults in Korea. Especially, global warming due to global climate change, especially El Niño phenomenon, and environmental pollution such as fine dust, There is no therapeutic or functional food.
현재 해외에서 안구건조증이나 눈 피로 관련 기능성식품이 대량 수입되고 있는 상황이다. 국내에서 우수한 기능성 식품이 개발될 경우 눈피로, 안구건조증으로 이행되는 것을 예방함으로 국민 개인의 건강뿐만 아니라 막대한 국가 재정 손실을 방지할 수 있다.Currently, there are large-scale imports of dry eye syndrome and eye fatigue-related functional foods overseas. When functional food products developed in Korea are prevented from being transferred to eye fatigue and dry eye syndrome, it is possible to prevent national health loss as well as national financial loss.
작약(Paeonia lactiflora)은 작약과(Paeoniaceae)에 속하는 다년생 초본으로 중국의 일부와 시베리아 및 한국의 일대에 자생하고 있으며, 한약규격집에는 백작약과 적작약을 Paeonia lactiflora Pallas 또는 기타 동속식물의 뿌리라고 정의하고 있다. 작약의 약리활성은 혈소판응집억제 작용, 순환계에 대한 작용, 항종양작용, 면역증강작용 등이 보고되어 있다(손동주, 한국식품영양과학회지, 2002, 31, 511; 이보미, 대한약학회지, 2008, 4, 445). Paeonia lactiflora is a perennial plant belonging to the family Paeoniaceae and is native to parts of China, Siberia and Korea, and the herbal medicine and herbal medicines are defined as the roots of Paeonia lactiflora Pallas or other herbaceous plants . The pharmacological activities of peonies have been reported to inhibit platelet aggregation, to act on the circulatory system, to antitumor action, and to enhance immunity (Son Dong-Ju, Journal of the Korean Society of Food Science and Nutrition, 2002, 31, 511, 4, 445).
감초(Glycyrrhiza uralensis)는 콩과에 속하는 다년생 초본식물이며, 뿌리를 건조하여 약새로 사용할 수 있다. 감초의 약리활성은 해독작용에 의한 만성간염 개선, 항당뇨(Mae, Tatsumasa, et al., The Journal of nutrition 133.11 (2003): 3369-3377.), 항궤양 효과(Hong, Jae Seung, et al., Korean Journal of Plant Resources 27.5 (2014): 401-414.), 궤양성 대장염 억제 효과(Lee, Keyong-Ho, and Ki-Hyeong Rhee., The Korean Journal of Food and Nutrition 23.4 (2010): 435-439.), 항산화 효과(Woo, Koan-Sik, et al., Journal of the Korean Society of Food Science and Nutrition 36.6 (2007): 689-695.) 등이 보고되어 있다.Licorice ( Glycyrrhiza uralensis ) is a perennial herbaceous plant belonging to the soybean family. The pharmacological activity of licorice was evaluated by the antitumor effect of chronic hepatitis improvement, antidiabetic effect (Mae, Tatsumasa, et al., The Journal of nutrition 133.11 (2003): 3369-3377) , Korean Journal of Plant Resources 27.5 (2014): 401-414.), Ulcerative Colitis Inhibitory Effect (Lee, Keyong-Ho, and Ki-Hyeong Rhee., The Korean Journal of Food and Nutrition 23.4 -439.), Antioxidant effects (Woo, Koan-Sik, et al., Journal of the Korean Society of Food Science and Nutrition 36.6 (2007): 689-695).
이에 본 발명자들은 작약 및 감초의 혼합 추출물 또는 이의 분획물이 눈물 분비량의 증가 및 각막형태 변화의 억제를 통해 안구건조증을 치료할 수 있음을 확인하여, 본 발명을 완성하였다.Accordingly, the present inventors have completed the present invention by confirming that a mixed extract of peony root and licorice, or a fraction thereof, can treat dry eye syndrome through an increase in tear secretion amount and inhibition of corneal morphological change.
본 발명의 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 치료용 약학 조성물을 제공하는 것이다.It is an object of the present invention to provide a pharmaceutical composition for preventing or treating dry eye syndrome which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
본 발명의 다른 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 윳효성분으로 포함하는 조성물의 안구건조증 예방 또는 치료 용도를 제공하는 것이다.Another object of the present invention is to provide a composition for preventing or treating dry eye syndrome comprising a mixture of peony root and licorice root extract or a fraction thereof as an active ingredient.
본 발명의 또 다른 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 개선용 건강기능식품을 제공하는 것이다.Another object of the present invention is to provide a health functional food for preventing or ameliorating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
본 발명의 또 다른 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 개선용 사료 조성물을 제공하는 것이다.It is another object of the present invention to provide a feed composition for preventing or improving dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
본 발명의 또 다른 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 치료용 의약외품 조성물을 제공하는 것이다.Another object of the present invention is to provide a quasi-drug composition for the prevention or treatment of dry eye syndrome comprising a mixture of peony root and licorice root extract or a fraction thereof as an active ingredient.
본 발명의 또 다른 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 개선용 눈 화장료 첨가제 조성물을 제공하는 것이다.Another object of the present invention is to provide an eye cosmetic additive composition for preventing or improving dry eye syndrome, which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
본 발명의 또 다른 하나의 목적은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 개체에 투여하는 단계를 포함하는 안구건조증 예방 또는 치료방법을 제공하는 것이다.Another object of the present invention is to provide a method for preventing or treating dry eye syndrome comprising administering to a subject a mixed extract of licorice and licorice or a fraction thereof.
본 발명의 작약 및 감초의 혼합 추출물 및 이의 분획물은 안구건조증에 의한 눈물 분비량의 감소 및 각막 형태의 변화를 유의하게 억제할 수 있으므로, 안구건조증의 예방 또는 치료를 위한 약학 조성물의 개발에 널리 활용될 수 있을 것이다.The peanut and licorice mixed extract of the present invention and the fractions thereof can be used for the development of a pharmaceutical composition for the prevention or treatment of dry eye syndrome since the amount of tear secretion due to dry eye syndrome and the change of corneal morphology can be significantly inhibited It will be possible.
도 1은 혼합 추출물 투여에 따른 눈물 분비량 변화를 눈물량 검사지를 통해 확인한 사진이다.FIG. 1 is a photograph showing a change in the amount of tear secretion according to the administration of the mixed extract through an eye volume test sheet.
도 2는 혼합 추출물 투여에 따른 눈물 분비량 변화를 정량적으로 나타낸 그래프이다 (*: p<0.05 vs. Normal; #: p<0.05 vs. dry eye).FIG. 2 is a graph showing a quantitative change in the amount of tear secretion according to the mixed extract administration (*: p <0.05 vs. Normal; #: p <0.05 vs. dry eye).
도 3은 혼합 추출물 투여에 따른 랫트의 각막형태 변화를 나타내는 사진이다.FIG. 3 is a photograph showing changes in the corneal morphology of rats upon administration of mixed extracts.
도 4는 혼합 추출물 투여에 따른 랫트의 각막 평활도(corneal smoothness score) 점수를 나타낸 그래프이다. 상기 각막 평활도 점수는 각막 형태 변화를 둥근 원 형태의 찌그림 정도를 0 내지 5까지 점수를 매겨 평가하였다(0점: 찌그러짐 없음, 1점: 1/4 부위에서 찌그러짐, 2점: 2/4 부위에서 찌그러짐, 3점: 3/4 부위에서 찌그러짐, 4점: 모든 부위에서 찌그러짐, 5점: 심한 찌그러짐으로 둥근 원형의 형태를 알아볼 수 없음; #: p<0.05 vs. dry eye).FIG. 4 is a graph showing scores of corneal smoothness score in rats according to the mixed extract administration. The corneal smoothness score was evaluated by dividing the corneal morphology by 0 to 5 (zero point: no distortion, 1 point: distortion at 1/4 point, 2 points: 2/4 point , 3 points: Distortion at 3/4 point, 4 points: Distortion at all points, 5 points: Uncertainty of rounded shape due to severe distortion, #: p <0.05 vs. dry eye).
도 5는 감초 추출물 투여에 따른 눈물 분비량 변화를 정량적으로 나타낸 그래프이다 (*: p<0.05 vs. Normal).FIG. 5 is a graph showing a quantitative change in the amount of tear secretion according to administration of licorice extract (*: p <0.05 vs. Normal).
도 6은 감초 추출물 투여에 따른 랫트의 각막형태 변화를 나타내는 사진이다.6 is a photograph showing changes in the corneal morphology of rats upon administration of licorice extract.
도 7은 혼합 추출물 투여에 따른 랫트의 각막 평활도(corneal smoothness score) 점수를 나타낸 그래프이다 (*: p<0.05 vs. Normal)..FIG. 7 is a graph showing the corneal smoothness score of rats according to administration of mixed extract (*: p <0.05 vs. Normal).
본 발명자들은 안구건조증을 효과적으로 치료할 수 있는 치료제를 개발하고자, 다양한 연구를 수행하던 중, 작약 및 감초의 혼합 추출물 또는 이의 분획물이 안구건조증을 치료하는 효과를 나타냄을 발견하였다. 상기 작약 및 감초의 혼합 추출물 또는 분획물은 안구 건조증에 따라 나타나는 눈물 분비량의 감소 및 각막 형태의 변화를 유의하게 억제할 뿐만 아니라, 안구건조증의 치료를 위해 사용되고 있는 상용약물과 유사한 효과를 나타냄을 알 수 있었다. 상기 혼합 추출물 또는 분획물을 사용하여 안구건조증을 치료하는 기술은 지금까지 전혀 알려져 있지 않고, 본 발명자에 의하여 최초로 개발되었다. The inventors of the present invention have found that when a variety of studies are conducted to develop a therapeutic agent capable of effectively treating dry eye syndrome, a mixed extract of peony root and licorice or a fraction thereof shows an effect of treating dry eye syndrome. The above-mentioned mixed extracts or fractions of peanut and licorice not only significantly inhibited the decrease of tear secretion and changes in corneal morphology according to dry eye syndrome but also showed similar effects to the commercial drugs used for the treatment of dry eye syndrome there was. The technique for treating dry eye syndrome using the mixed extract or fraction has not been known at all and has been developed for the first time by the present inventors.
상기 목적을 달성하기 위한 일 실시양태로서, 본 발명은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 치료용 약학 조성물을 제공한다. In one aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating dry eye syndrome, which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
또한, 본 발명은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 조성물의 안구건조증 예방, 개선 또는 치료용도를 제공한다.The present invention also provides a composition for preventing, ameliorating or treating dry eye syndrome, comprising a mixture of peony root and licorice, or a fraction thereof as an active ingredient.
안구건조증의 예방 및 치료 활성을 갖는 상기 작약 및 감초의 혼합 추출물은 천연, 잡종, 변종 식물의 다양한 기관으로부터 추출될 수 있고, 예를 들어 뿌리, 줄기, 잎, 꽃, 열매의 몸통, 열매의 껍질뿐만 아니라 식물 조직 배양물로부터 추출 가능하다.The mixed extract of peanut and licorice having the preventive and therapeutic activity of dry eye syndrome can be extracted from various organs of natural, hybrid, and variant plants and can be extracted from various organs such as roots, stems, leaves, flowers, As well as extracts from plant tissue cultures.
본 발명에서 사용된 용어 "작약(Paeonia lactiflora)"은 작약과(Paeoniaceae)에 속하는 다년생 초본으로 중국의 일부와 시베리아 및 한국의 일대에 자생하고 있다. 한약규격집에는 작약을 Paeonia lactiflora 또는 기타 동속식물의 뿌리라고 정의하고 있으며, 껍질을 벗겨 건조한 것을 백작약, 껍질과 함께 건조한 것을 적작약이라 한다. 작약의 약리활성은 혈소판응집억제 작용, 순환계에 대한 작용, 항종양작용, 면역증강작용 등이 보고되어 있다.As used herein, the term " Paeonia lactiflora "is a perennial herb that belongs to the family Paeoniaceae and is native to parts of China, Siberia and Korea. In the Chinese medicine standard book, peonies are defined as the roots of Paeonia lactiflora or other plants, and peeled, dried and dried together with peel and peel are called peptone. The pharmacological activities of peonies have been reported to inhibit platelet aggregation, to act on the circulatory system, to antitumor action, and to enhance immunity.
본 발명에서 사용된 용어 "감초(Glycyrrhiza uralensis)"는 콩과에 속하는 다년생 초본식물이며, 뿌리를 건조하여 약재 또는 감미료로 사용할 수 있다. 감초의 약리활성은 해독작용에 의한 만성간염 개선, 항당뇨, 항궤양 효과, 궤양성 대장염 억제 효과, 항산화 효과 등이 보고되어 있다.As used herein, the term " Glycyrrhiza uralensis "is a perennial herbaceous plant belonging to the soybean family and may be used as a medicinal or sweetener by drying the roots. The pharmacological activity of licorice has been reported to improve chronic hepatitis by detoxification, anti - diabetic effect, anti - ulcer effect, ulcerative colitis inhibitory effect, antioxidant effect.
본 발명에서, 상기 혼합 추출물은 작약 및 감초의 뿌리, 줄기, 잎, 꽃, 또는 열매로부터 추출한 것일 수 있으며, 이에 특별히 제한되는 것은 아니다.In the present invention, the mixed extract may be extracted from roots, stems, leaves, flowers, or fruits of peony root and licorice, and is not particularly limited thereto.
본 발명에서, 상기 작약 및 감초의 혼합 추출물은 물, 탄소수 1 내지 4의 저급 알콜 및 이들의 혼합 용매로 구성되는 군으로부터 선택되는 용매로 추출한 것을 포함하나, 이에 제한되지 않는다. 또한, 본 발명에 있어서, 상기 추출물에는, 추출처리에 의해 얻어지는 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 얻어지는 건조물, 또는 이들 조정제물 또는 정제물 중 어느 하나도 포함될 수 있다.In the present invention, the mixed extract of peony root and licorice includes, but is not limited to, extracts from a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof. In addition, in the present invention, the above extract may contain any one of the extract obtained by the extraction treatment, the diluted solution or the concentrate of the extract, the dried product obtained by drying the extract, or the adjusted product or the purified product.
상기 알콜은 부틸 알콜, 에틸 알콜, 메틸 알콜 또는 이의 수용성 알콜인 것일 수 있다. 구체적으로 상기 수용성 알콜은 알콜이 0.1 내지 100 % 중량 범위로 포함되는 것 일 수 있다.The alcohol may be butyl alcohol, ethyl alcohol, methyl alcohol or a water-soluble alcohol thereof. Specifically, the water-soluble alcohol may be an alcohol in an amount of 0.1 to 100% by weight.
본 발명에서 사용되는 용어, "분획물"은 여러 다양한 구성 성분들을 포함하는 혼합물로부터 특정 성분 또는 특정 성분 그룹을 분리하기 위하여 분획을 수행하여 얻어진 결과물을 의미한다.The term "fraction " as used herein means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
본 발명에서 상기 분획물을 얻는 분획 방법은 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 수행될 수 있다. 상기 분획 방법의 비제한적인 예로는, 작약 및 감초의 혼합 추출물을 추출하여 얻은 추출물에 소정의 용매를 처리하여 상기 추출물로부터 분획물을 얻는 방법을 들 수 있다.The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, a method of obtaining a fraction from the extract by treating a predetermined solvent with an extract obtained by extracting a mixed extract of peony root and licorice root.
본 발명에 있어서, 상기 분획물은 작약 및 감초의 혼합 추출물의 헥산 분획물, 에틸 아세테이트 분획물, 부탄올 분획물 또는 물 분획물인 것일 수 있다. 상기 분획물은 각각 약학 조성물의 총 중량에 대하여 0.01 내지 100 중량%, 보다 구체적으로 1 내지 80 중량%로 포함될 수 있다.In the present invention, the fraction may be a hexane fraction, an ethyl acetate fraction, a butanol fraction or a water fraction of a mixture of peony root and licorice. The fractions may each comprise 0.01 to 100% by weight, more specifically 1 to 80% by weight, based on the total weight of the pharmaceutical composition.
본 발명에서 상기 분획물을 얻는 데에 사용되는 분획 용매의 종류는 특별히 제한되지 아니하며, 당해 기술 분야에서 공지된 임의의 용매를 사용할 수 있다. 상기 분획 용매의 비제한적인 예로는 물, 알코올 등의 극성 용매; 헥산(Hexan), 에틸 아세테이트(Ethyl acetate), 클로로포름(Chloroform), 디클로로메탄(Dichloromethane) 등의 비극성 용매 등을 들 수 있다. 이들은 단독으로 사용되거나 2 종 이상 혼합하여 사용될 수 있다. 상기 분획 용매 중 알코올을 사용하는 경우에는 구체적으로 C1 내지 C4의 알코올을 사용할 수 있다.The kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When an alcohol is used in the fraction solvent, C1 to C4 alcohols can be specifically used.
본 발명에서 사용된 용어 "안구건조증"이란, 안구건조증(dry eye syndrome)은 눈물샘의 염증 및 각막의 탈신경화에 의하여 눈물 분비가 억제되거나 마이봄선의 기능부전(Meibomian gland dysfunction) 또는 눈꺼풀의 기능이상에 따른 비정상적인 눈물 증발 등에 의하여 발생하는 질환일 수 있고, 상기 안구건조증에 의해 눈물이 과다 건조되어 각막의 상처로 나타나는 각결막 상피장애일 수 있다.As used herein, the term "dry eye syndrome" refers to dry eye syndrome, which is caused by inflammation of the lacrimal gland and decongestion of the cornea, inhibiting tear secretion, And abnormal conjunctival epithelial disorder caused by excessive dryness of the tears due to the dry eye syndrome and resulting in wounds of the cornea.
본 발명에서 사용된 용어 "예방"이란, 본 발명에 따른 작약 및 감초의 혼합 추출물 또는 이의 분획물을 포함하는 조성물의 투여로 안구건조증의 증상을 억제 또는 지연시키는 모든 행위를 말한다.As used herein, the term "prevention" refers to any act that inhibits or delays the symptoms of dry eye syndrome by administration of a composition comprising the peony root extract of licorice and licorice, or fractions thereof, according to the present invention.
본 발명에서 사용된 용어 "치료"란, 본 발명에 따른 작약 및 감초의 혼합 추출물 또는 이의 분획물을 포함하는 조성물의 투여로 상기 안구건조증의 증상이 호전되거나 이롭게 변경되는 모든 행위를 말한다.As used herein, the term "treatment" refers to any action that improves or alleviates the symptoms of dry eye syndrome by administration of a composition comprising the peony root extract and licorice root extract or fractions thereof according to the present invention.
본 발명에서 상기 조성물은 눈물의 분비량을 증가시키거나 각막의 형태변화를 억제시키는 것일 수 있다. 상기 각막의 형태 변화는 눈물의 과다건조로 인해 각막의 표면이 울퉁불퉁해져 둥근 원형이 휘어지고 찌그러지는 형태를 나타내는데 본 발명의 조성물은 이를 매끈하고 평평하게 개선시키는 것일 수 있다.In the present invention, the composition may increase the secretion amount of tears or inhibit the morphological change of the cornea. The morphological change of the cornea may be due to excessive irritation of the tears, which may cause the surface of the cornea to become rugged and the rounded circle to warp and distort. The composition of the present invention may improve smoothness and flatness.
본 발명의 일 실시예에서 작약 및 감초의 혼합 추출물을 투여하여, 모든 농도군에서 유의적으로 눈물 분비량이 증가함을 확인하였고, 농도의존적으로 그 효과가 증가하는 것을 확인하였다(도 1 및 도 2). In one embodiment of the present invention, it was confirmed that the tear secretion amount was significantly increased in all the concentration groups when the mixed extract of peony root and Licorice was administered, and it was confirmed that the effect was increased depending on the concentration (FIGS. 1 and 2 ).
본 발명의 다른 일 실시예에서 작약 및 감초의 혼합 추출물을 투여하여, 고농도군(100mg/kg 및 250mg/kg)에서 안구건조증 질환군에서 나타나는 각막 형태의 변화를 유의적으로 억제함을 확인하였다(도 3 및 4).In another embodiment of the present invention, it was confirmed that administration of a mixture of peony and licorice extract significantly inhibited the change in corneal morphology in the dry eye syndrome group at high concentrations (100 mg / kg and 250 mg / kg) 3 and 4).
따라서, 상기 결과를 통해서 작약 및 감초의 혼합 추출물이 안구건조증에 대해 치료효과를 가짐을 알 수 있었다.Therefore, it can be seen from the above results that the mixed extract of peony root and licorice has a therapeutic effect on dry eye syndrome.
본 발명에서 사용된 용어 "약학 조성물"이란, 질병의 예방 또는 치료를 목적으로 제조된 것을 의미하며, 각각의 통상의 방법에 따라 다양한 형태로 제형화하여 사용될 수 있다. 예컨대, 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽 등의 제형으로 제형화할 수 있고, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. 구체적으로, 점안투여하기 적합한 형태인 안구 외용제형, 예를 들어, 점안제, 크림제, 연고제, 겔제 또는 로션제로 제형화하여 사용될 수 있다.As used herein, the term "pharmaceutical composition" means a preparation intended for the prevention or treatment of disease, and may be formulated into various forms according to each ordinary method. For example, it can be formulated into a form such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and the like, and can be formulated in the form of external preparations, suppositories and sterilized injection solutions. Specifically, it can be formulated into an external preparation for eye, a form suitable for topical administration, for example, an eye drop, a cream, an ointment, a gel or a lotion.
상기 본 발명의 조성물은, 약학적 조성물의 제조에 통상적으로 사용하는 적절한 담체, 부형제 또는 희석제를 추가로 포함하는 안구건조증 예방 또는 치료용 약학 조성물의 형태로 제조될 수 있는데, 상기 담체는 비자연적 담체(non-naturally occuring carrier)를 포함할 수 있다. The composition of the present invention may be prepared in the form of a pharmaceutical composition for preventing or treating dry eye syndrome, which further comprises an appropriate carrier, excipient or diluent conventionally used in the production of a pharmaceutical composition, (non-naturally occuring carrier).
본 발명에서, 상기 약학 조성물에 포함될 수 있는 담체, 부형제 및 희석제로는 락토즈, 덱스트로즈, 수크로스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로스, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유를 들 수 있다.In the present invention, the carrier, excipient and diluent which may be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.
제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 상기 상엽 추출물과 이의 분획물들에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트(calcium carbonate), 수크로스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제된다. 또한 단순한 부형제 이외에 마그네슘 스티레이트, 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는 데 흔히 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구 투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함된다. 비수성용제, 현탁제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다.In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use may include various excipients such as wetting agents, sweetening agents, fragrances, preservatives, etc. in addition to water and liquid paraffin, which are simple diluents commonly used in suspension, liquid solutions, emulsions and syrups have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
본 발명의 약학 조성물의 투여량은 사용목적, 질환의 중독도, 환자의 연령, 체중, 성별, 기왕력, 또는 유효성분으로서 사용되는 물질의 종류 등을 고려하여 당업자가 결정할 수 있다. 예를 들어, 본 발명의 약학 조성물은 성인 1인당 약 0.1 ng 내지 약 1,000mg/kg, 구체적으로 1 ng 내지 약 1,000mg/kg로 투여할 수 있고, 본 발명의 조성물의 투여빈도는 특별히 이에 제한되지 않으나, 1일 1회 투여하거나 또는 용량을 분할하여 수회 투여할 수 있다. 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다.The dosage of the pharmaceutical composition of the present invention can be determined by those skilled in the art in consideration of the purpose of use, the degree of addiction to the disease, the age, body weight, sex, history, or kind of the substance used as the active ingredient. For example, the pharmaceutical composition of the present invention may be administered at a dose of from about 0.1 ng to about 1,000 mg / kg, in particular from 1 ng to 1,000 mg / kg, per adult, and the frequency of administration of the composition of the present invention is specifically limited However, it may be administered once a day or divided into several doses. The dose is not intended to limit the scope of the invention in any way.
다른 하나의 양태로서, 본 발명은 상기 약학 조성물을 약제학적으로 유효한 양으로 안구 건조증이 발병된 개체에 투여하는 단계를 포함하는 안구 건조증의 치료방법을 제공한다. In another aspect, the present invention provides a method for treating dry eye syndrome comprising administering the pharmaceutical composition to a subject suffering from dry eye syndrome in a pharmaceutically effective amount.
본 발명에서 사용된 용어 "개체"란 안구건조증이 발병된 쥐, 가축 등을 포함하는 포유동물 등을 제한 없이 포함할 수 있다.The term "individual" as used herein may include, without limitation, mammals including rats, livestock, and the like with dry eye syndrome.
보다 구체적으로, 상기 "개체"는 반려동물을 포함할 수 있다. 상기 반려동물은 인간과 함께 더불어 사는 동물을 의미하며, 구체적인 종류로서 개, 고양이, 햄스터, 기니피그 등의 포유류, 앵무새, 카나리아 등의 조류 등을 들 수 있으나, 이에 제한되는 것은 아니다.More specifically, the "individual" may include companion animals. The companion animal refers to an animal living together with a human, and specific examples include, but are not limited to, mammals such as dogs, cats, hamsters, and guinea pigs, and birds such as parrots and canaries.
상기 조성물은 약학적으로 유효한 양으로 단일 또는 다중 투여될 수 있다.The composition may be administered in single or multiple doses in a pharmaceutically effective amount.
본 발명의 안구건조증의 예방 또는 치료용 약학 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 어떠한 일반적인 경로를 통하여도 투여될 수 있다. The administration route of the pharmaceutical composition for preventing or treating dry eye syndrome of the present invention can be administered through any ordinary route as long as it can reach the target tissue.
본 발명의 약학 조성물은 특별히 이에 제한되지 않으나, 목적하는 바에 따라 점안 투여, 복강내 투여, 정맥내 투여, 근육내 투여, 피하 투여, 피내 투여, 경피패치투여, 경구 투여, 비내 투여, 폐내 투여, 직장내 투여 등의 경로를 통해 투여 될 수 있고, 구체적으로 경구 투여의 경로를 통해 투여될 수 있다. The pharmaceutical composition of the present invention may be administered orally, intraperitoneally, intramuscularly, subcutaneously, intradermally, transdermal patch, oral, intranasal, intrapulmonary, Intramuscular administration, intrathecal administration, intrarectal administration, and the like, and specifically administered orally.
또 다른 하나의 양태로서, 본 발명은 작약 및 감초의 혼합 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 개선용 건강기능식품을 제공한다.In another aspect, the present invention provides a health functional food for preventing or ameliorating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
본 발명의 건강기능식품에서 작약, 감초, 분획물, 안구건조증에 대한 정의는 상기 정의된 바와 같다. The definition of peanut, licorice, fraction, and dry eye syndrome in the health functional food of the present invention is as defined above.
본 발명에서 사용된 용어 "개선"은 상기 조성물을 경구투여하여 안구건조증이 호전되거나 이롭게 변경되는 모든 행위를 의미한다.As used herein, the term "improvement" means any action that alters or modifies dryness of the eye by orally administering the composition.
본 발명에서 사용된 용어 "기능성"은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻는 것을 의미한다. The term "functional" as used in the present invention means that the structure and function of the human body have a beneficial effect for health use such as controlling nutrients or physiological action.
본 발명의 건강기능식품은 당 업계에서 통상적으로 사용되는 방법에 의하여 제조 가능하며, 상기 제조시에는 당 업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. 또한, 상기 건강기능식품의 제형 또한 건강기능식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 본 발명의 건강기능식품은 다양한 형태의 제형으로 제조될 수 있으며, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고 휴대성이 뛰어나므로, 본 발명의 건강기능식품은 안구건조증의 예방 또는 개선의 효과를 증진시키기 위한 보조제로 섭취가 가능하다.The health functional food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients that are conventionally added in the art. In addition, the formulation of the health functional food may also be produced without limitation as long as the formulation is recognized as a health functional food. The health functional food of the present invention can be manufactured in various forms, and unlike general pharmaceuticals, it has advantages of being free from side effects that may occur when a food is used as a raw material for a long period of time, and is excellent in portability, Can be ingested as an adjuvant to improve the effect of preventing or improving dry eye syndrome.
구체적으로, 상기 건강기능식품은 본 발명의 혼합 추출물 또는 분획물을 음료, 차류, 향신료, 껌, 과자류 등의 식품 소재에 첨가하거나, 캡슐화, 분말화, 현탁액 등으로 제조한 식품으로, 이를 섭취할 경우 건강상 특정한 효과를 가져오는 것을 의미하나, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용이 없는 장점이 있다.Specifically, the health functional food is a food prepared by adding the mixed extract or fraction of the present invention to a food material such as beverage, tea, spice, gum, confectionery, or the like, or encapsulating, powdering, or suspending, But it has the advantage that there is no side effect that can occur when a drug is taken for a long time using a food as a raw material, unlike a general medicine.
본 발명의 또 다른 하나의 양태로서, 벌개미취 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증의 예방 또는 개선용 눈 화장료 첨가제 조성물을 제공한다.As another embodiment of the present invention, there is provided an eye cosmetic additive composition for preventing or improving dry eye syndrome comprising extracts of Euphorbiaceae or fractions thereof as an active ingredient.
상기 눈 화장료 첨가제 조성물에서 벌개미취 추출물, 분획물, 안구건조증, 예방 및 개선에 대한 정의는 상기 정의된 바와 같다.The definition of the extract of Eisenhower extract, fraction, dry eye syndrome, prevention and improvement in the eye cosmetic additive composition is as defined above.
상기 첨가제는 눈 화장용 제품류에 포함되어 상기 질환의 예방 또는 개선에 효과를 나타낼 수 있다. 본 발명에서 상기 조성물은 눈 화장용 제품류를 포함하는 것일 수 있으며, 상기 눈 화장료 제품류로는 아이브로 펜슬, 아이 라이너, 아이섀도, 마스카라, 및 아이 메이크업 리무버를 포함하는 군으로부터 선택되는 어느 하나인 것일 수 있다.The additive may be included in eye make-up products and may be effective in preventing or improving the disease. In the present invention, the composition may include eye make-up products. Examples of the eye make-up cosmetic products include any one selected from the group consisting of eye pencil, eye liner, eye shadow, mascara, and eye makeup remover. have.
본 발명의 또 다른 하나의 양태로서, 벌개미취 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증의 예방 또는 개선용 의약외품 조성물을 제공한다.In another aspect of the present invention, there is provided a quasi-drug composition for preventing or improving dry eye syndrome comprising extracts of Euphorbiaceae or fractions thereof as an active ingredient.
상기 의약외품 조성물에서 벌개미취 추출물, 분획물, 안구건조증, 예방 및 개선에 대한 정의는 상기 정의된 바와 같다.In the above-mentioned quasi-drug composition, the definitions of the extracts of Echinochloa sp., Fractions, dry eye syndrome, prevention and improvement are as defined above.
본 발명에서 용어, "의약외품"은 사람이나 동물의 질병을 진단, 치료, 개선,경감, 처치 또는 예방할 목적으로 사용되는 물품들 중 의약품보다 작용이 경미한 물품들을 의미하는 것으로, 예를 들어 약사법에 따르면 의약외품이란 의약품의 용도로 사용되는 물품을 제외한 것으로, 사람ㆍ동물의 질병 치료나 예방에 쓰이는 섬유 ㆍ고무 제품, 인체에 대한 작용이 경미하거나 직접 작용하지 않으며, 기구 또는 기계가 아닌 것과 이와 유사한 것, 감염병을 막기 위한 살균ㆍ살충제 등이 이에 포함된다. 본 발명의 의약외품 조성물의 종류나 제형은 특별히 제한되지 아니하나, 바람직하게는 소독 청결제, 샤워폼, 가그린, 물티슈, 세제 비누, 핸드 워시, 가습기 충진제, 마스크, 연고제 또는 필터 충진제 등일 수 있다.The term "quasi-drug product" in the present invention means products which are less active than drugs, for example, for the purpose of diagnosing, treating, improving, alleviating, treating or preventing a disease in a human or an animal. For example, Quasi-drugs are products that are not used for medicines, such as fibers and rubber products used for the treatment and prevention of diseases of humans and animals, and are not acting directly or indirectly on the human body, These include sterilization and insecticides to prevent infectious diseases. The type and formulations of the quasi-drug composition of the present invention are not particularly limited, but may be disinfectant cleaner, shower foam, gagrin, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment or filter filler.
본 발명의 또 다른 하나의 양태로서, 벌개미취 추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증의 예방 또는 개선용 사료 조성물을 제공한다.In another aspect of the present invention, there is provided a feed composition for preventing or ameliorating dry eye syndrome comprising extracts of Euphorbiaceae or fractions thereof as an active ingredient.
상기 조성물에서 벌개미취 추출물, 분획물, 안구건조증, 예방 및 개선에 대한 정의는 상기 정의된 바와 같다.The definition of the extract of Echinochloa sp., Fractions, dry eye syndrome, prevention and improvement in the composition is as defined above.
본 발명에서 용어, "사료"는 개체가 먹고, 섭취하며, 소화시키기 위한 또는 이에 적당한 임의의 천연 또는 인공 규정식, 한끼식 등 또는 상기 한끼식의 성분을 의미한다.As used herein, the term "feed" means any natural or artificial diet, single meal or the like or ingredients of the above formula for ingesting, ingesting, digesting or suitable for the individual.
상기 사료의 종류는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 사료를 사용할 수 있다. 상기 사료의 비제한적인 예로는, 곡물류, 근과류, 식품 가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박 류 또는 곡물 부산물류 등과 같은 식물성 사료; 단백질류, 무기물류, 유지류, 광물성류, 유지류, 단세포 단백질류, 동물성 플랑크톤류 또는 음식물 등과 같은 동물성 사료를 들 수 있다. 이들은 단독으로 사용되거나 2종 이상을 혼합하여 사용될 수 있다.The kind of the feed is not particularly limited, and feeds conventionally used in the art can be used. Non-limiting examples of such feeds include vegetable feeds such as cereals, muscle roots, food processing busines logistics, algae, fibers, pharmaceuticals, buses, oils, pastes, pulses or cereals; Animal feeds such as proteins, inorganic substances, fats, oils, fats, oils, monocellular proteins, animal plankton or foods. These may be used alone or in combination of two or more.
본 발명의 일 실시예에서 작약 및 감초의 혼합 추출물을 투여하여, 모든 농도군에서 유의적으로 눈물 분비량이 증가함을 확인하였고, 농도의존적으로 그 효과가 증가하는 것을 확인하였다(도 1 및 도 2).In one embodiment of the present invention, it was confirmed that the tear secretion amount was significantly increased in all the concentration groups when the mixed extract of peony root and Licorice was administered, and it was confirmed that the effect was increased depending on the concentration (FIGS. 1 and 2 ).
본 발명의 다른 일 실시예에서 작약 및 감초의 혼합 추출물을 투여하여, 고농도군(100mg/kg 및 250mg/kg)에서 안구건조증 질환군에서 나타나는 각막 형태의 변화를 유의적으로 억제함을 확인하였다(도 3 및 4).In another embodiment of the present invention, it was confirmed that administration of a mixture of peony and licorice extract significantly inhibited the change in corneal morphology in the dry eye syndrome group at high concentrations (100 mg / kg and 250 mg / kg) 3 and 4).
따라서, 상기 결과를 통해서 작약 및 감초의 혼합 추출물 또는 이의 분획물은, 안구건조증 또는 눈피로 예방, 개선 또는 개선치료용 약학적 조성물, 건강기능식품, 눈 화장료 첨가제, 의약외품 조성물 및 사료 조성물 건강기능식품으로 유용하게 활용될 수 있음을 알 수 있었다.Accordingly, the above-mentioned results indicate that the mixed extract of peony root and licorice or its fractions can be used as a pharmaceutical composition for preventing dry eye disease or eye fatigue, for improving or ameliorating eye fatigue, a health functional food, an eye cosmetic additive, And it can be used effectively.
이하, 본 발명을 실시예를 통하여 보다 상세하게 설명한다. 그러나 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다. Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.
실시예 1. 작약 및 감초의 혼합 추출물 제조Example 1. Preparation of mixed extract of peony and licorice
작약과 감초를 2:1의 비율로 칭량한 후, 정제수를 넣고 약 90℃ 에서 8시간 동안 환류추출하여 얻은 추출물을 여과하고 약 50℃에서 감압농축한 후 건조하여 혼합 추출물을 제조하였다.The extracts were filtered, and the filtrate was concentrated under reduced pressure at about 50 ° C and dried to prepare a mixed extract.
실시예 2. 실험동물을 이용한 약효시험Example 2. Drug test using an experimental animal
실시예 2.1: 안구건조증 실험동물의 제작Example 2.1: Dry eye syndrome Production of experimental animals
6주령 암컷 SD 랫트(오리엔트바이오, 한국)를 구입하고 일주일 순화 후, 눈물샘 제거를 위해 펜토바비탈 나트륨(pentobarbital sodium(30 mg/kg body weight, 한림제약, 한국)으로 마취하였다. 마취시킨 랫트의 안구와 귀 사이 정중앙 부위의 털을 제모하고, 수술부위를 소독한 후 피부를 1cm 가량 절개하였다. 절개 부위로부터 안구 밖 눈물샘(exorbital lacrimal gland)를 떼어낸 후, 피부를 봉합하였다. 1주일 뒤, 페놀-레드 thread 눈물량 검사지(FCI Opthalmics Zone Quick, Japan)를 눈꺼풀 외측 끝 부위 안구표면에 접촉시킨 후 30초 후 눈물에 의해 검사지의 색이 변한 길이를 측정하여, 비수술군에 비해 현저하게 눈물 분비량이 감소된 개제들만 이용하여 약효시험을 진행하였다.Six weeks old female SD rats (Orient Bio, Korea) were purchased and anesthetized with pentobarbital sodium (30 mg / kg body weight, Hanlim Pharm, Korea) for lacrimal clearance after one week purification. After removing the exorbital lacrimal gland from the incision site, the skin was sutured.After 1 week, the skin was sutured to the skin, Phenol-red thread volume test (FCI Opthalmics Zone Quick, Japan) was applied to the eyeball surface at the lateral end of the eyelid. After 30 seconds, the change in color of the test piece was measured by the tear test. The drug efficacy test was conducted using only these reduced preparations.
실시예 2.2: 눈물 분비량 측정Example 2.2: Tear secretion measurement
안구건조증에 대한 혼합 추출물의 효과를 확인하기 위하여, 상기 실시예 2.1의 실험동물을 이용하여 혼합 추출물 투여에 따른 눈물 분비량의 변화를 확인하였다. In order to confirm the effect of the mixed extract on the dry eye syndrome, the change of the tear secretion amount according to the mixed extract administration was confirmed using the experimental animal of Example 2.1.
각 실험군별로 5마리의 개체를 무작위로 배정하였으며, 실험군은 다음과 같이 구성하였다: 아무 이상이 없는 비수술 정상군 (NOR), 실시예 2.1의 방법으로 안구 밖 눈물샘을 제거하여 안구건조증을 유발시킨 질환군 (DED), 본 발명의 혼합 추출물(JGT)을 50 mg/kg을 투여한 약물투여군 (JGT-50), 본 발명의 혼합 추출물(JGT)을 100 mg/kg을 투여한 약물투여군 (JGT-100), 본 발명의 혼합 추출물(JGT)을 250 mg/kg을 투여한 약물투여군 (JGT-250). 본 발명의 혼합 추출물을 포함하는 약물은 0.5 % DMSO가 포함된 멸균 생리식염수에 상기 투여량을 녹여 준비하였으며, 4일 동안 경구 투여 후 투여 5일째 안구건조증을 유발 3일 동안 추가 투여 하였다. 총 투여 7일째 실험동물을 마취 시키고, 페놀-레드 thread 눈물량 검사지(FCI Opthalmics Zone Quick,Japan)를 눈꺼풀 외측 끝 부위 안구표면에 접촉시킨 후 30초 후 눈물에 의해 검사지의 색이 변한 길이를 측정하여 약효 효능 평가를 실시하였다.Five individuals were randomly assigned to each experimental group. The experimental group consisted of the following: non-invasive normal group (NOR) with no abnormality, the disease caused by dry eye lacrimal gland by the method of Example 2.1 (JGT-50) administered with 50 mg / kg of the mixed extract of the present invention (JGT), a drug administration group (JGT-50) administered with 100 mg / kg of the mixed extract of the present invention (JGT- 100), the drug-administered group (JGT-250) in which 250 mg / kg of the mixed extract of the present invention (JGT) was administered. The drug containing the mixed extract of the present invention was prepared by dissolving the above dose in sterilized physiological saline containing 0.5% DMSO. After the oral administration for 4 days, the dry eye was additionally administered on the 5th day for 3 days. On the 7th day after total administration, the animals were anesthetized and the change in color of the test strip was measured by tearing after 30 seconds of contact with the phenol-red thread volume test (FCI Opthalmics Zone Quick, Japan) And the efficacy of the drug was evaluated.
그 결과, 도 1 및 도 2에 나타낸 바와 같이, 정상군에서는 색이 변한 길이가 매우 긴 것에 비해, 안구건조증 질환군은 길이가 짧은 것을 확인하였다. 약물 투여군은 안구건조증 질환군과 비교하여 모든 실험 농도에서 그 길이가 유의적으로 늘어났으며, 농도의존적으로 증가하는 것을 확인하였다. As a result, as shown in Fig. 1 and Fig. 2, it was confirmed that the length of the color change in the normal group was very long, while that of the dry eye disease group was short. Compared with dry eye disease group, drug - treated group showed significant increase in length at all experimental concentrations and increased dose - dependently.
따라서, 상기 결과를 통해서, 작약 및 감초의 혼합 추출물을 투여하여 눈물 분비량이 개선됨을 확인하였으므로, 상기 혼합 추출물이 안구건조증 치료효과가 있음을 알 수 있었다. Thus, through the above results, it was confirmed that the tear secretion amount was improved by administering the mixed extract of peony and licorice, so that the mixed extract was found to be effective in treating dry eye syndrome.
실시예 2.3: 각막의 형태 변화 측정 Example 2.3: Measurement of morphological change of cornea
안구건조증에 대한 혼합 추출물의 효과를 확인하기 위하여, 상기 실시예 2.1의 실험동물을 이용하여 혼합 추출물 투여에 따른 각막의 형태 변화를 확인하였다.In order to confirm the effect of the mixed extract on the dry eye syndrome, the morphological changes of the cornea according to administration of the mixed extract were confirmed using the experimental animal of Example 2.1.
실험군의 구성 및 약물의 투여는 상기 실시예 2.2와 동일하게 수행하였다. The composition of the test group and administration of the drug were carried out in the same manner as in Example 2.2.
각막은 동물을 마취 후, 원형 광섬유 조명기(fiberoptic ring illuminator)가 부착된 입체현미경(stereoscopic microsope, Olympus, Japan)을 이용하여 사진을 찍었다. 각막의 형태 변화는 각막 상피세포에 반사된 원형 조명의 둥근 원 형태에 대한 찌그러짐 정도를 0 내지 5까지 점수를 매겨 평가하였다. 0점: 찌그러짐 없음, 1점: 1/4 부위에서 찌그러짐, 2점: 2/4 부위에서 찌그러짐, 3점: 3/4 부위에서 찌그러짐, 4점: 모든 부위에서 찌그러짐, 5점: 심한 찌그러짐으로 둥근 원형의 형태를 알아볼 수 없음. The animals were anesthetized and photographed using a stereoscopic microsope (Olympus, Japan) equipped with a fiberoptic ring illuminator. Changes in corneal morphology were assessed by grading the degree of distortion of the rounded circle shape of the circular light reflected on the corneal epithelium from 0 to 5. 0 point: No distortion, 1 point: Distortion at 1/4 point, 2 points: Distortion at 2/4 point, 3 points: Distortion at 3/4 point, 4 points: Distortion at all points, 5 point: Distortion at severe point Can not recognize the shape of the round circle.
각막의 형태학적 변화를 평가한 결과, 정상군에서는 외부에 눈물샘에서 분비되는 눈물이 tear film을 형성하여 매끈하고 평평한 표면 상태가 유지됨으로써, 도 3과 같이 원형이 조명 그대로 반사되어 둥근 원모양을 관찰하였다. 그러나 안구건조증 질환군에서는 각막을 덮고 있는 tear film에 이상이 생겨 각막 상피세포의 변성 및 매끈한 각막 표면이 말라서 울퉁불퉁하게 변하게 되어, 둥근 원형이 휘어지고 찌그러지는 형태를 관찰한 반면, JGT 약물의 고농도 투여군(JGT-100 및 JGT-250) 에서는 질환군과 같은 각막형태 변화를 유의적으로 억제하는것을 확인하였다(도 3 및 도 4). The morphological changes of the cornea were evaluated. In the normal group, the tears secreted from the external lacrimal gland formed a tear film, and the smooth and flat surface state was maintained. As a result, Respectively. However, in the dry eye syndrome group, abnormalities in the tear film covering the cornea resulted in degeneration of the epithelium of the corneal epithelium and smooth surface of the cornea, so that the rounded shape was distorted and distorted. On the other hand, (JGT-100 and JGT-250) significantly inhibited corneal morphological changes such as the disease group (FIGS. 3 and 4).
따라서, 상기 결과를 통해서, 작약 및 감초의 혼합 추출물을 투여하여 안구건조증에 의해 나타나는 각막의 형태변화를 억제함을 확인하였으므로, 상기 혼합 추출물이 안구건조증 치료효과가 있음을 알 수 있었다. Accordingly, it was confirmed through the above results that the combined extract of peony root and licorice inhibited the change of corneal morphology caused by dry eye syndrome. Thus, it was found that the mixed extract was effective in treating dry eye syndrome.
비교예 1.감초 추출물의 약효시험Comparative Example 1: Drug test for licorice root extract
비교예 1.1 감초 추출물 투여군의 눈물 분비량 측정Comparative Example 1.1 Measurement of tear secretion of licorice extract-treated group
감초 단독으로 실시예 1의 방법을 이용하여 추출한 감초 추출물의 안구건조증 치료 효과를 확인하기 위해, 실시예 2.2의 방법을 이용하여 눈물 분비량을 측정하였다. 감초 추출물의 투여량은 JGT-250(250mg/kg 투여) 기준 감초의 투여량인 84mg/kg을 투여하였다.The tear secretion amount was measured using the method of Example 2.2 in order to confirm the therapeutic effect of the licorice extract extracted by the method of Example 1 with dry liquorice alone. The dose of licorice extract was 84 mg / kg, the dose of Licorice based on JGT-250 (250 mg / kg dose).
그 결과, 도 5에 나타낸 바와 같이, 안구건조증 질환군과 비교하여 감초 추출물 투여군의 눈물 분비량이 유의적인 차이를 보이지 않아 감초 추출물이 눈물분비량을 증가시키지 않는 것을 확인하였다.As a result, as shown in FIG. 5, the tear secretion amount of the licorice extract-treated group was not significantly different from that of the dry eye syndrome group, confirming that the licorice extract did not increase the tear secretion amount.
비교예 1.2 감초 추출물 투여군의 각막 형태변화 측정Comparative Example 1.2 Measurement of corneal morphological change in licorice extract-treated group
감초 추출물의 안구건조증 치료효과를 확인하기 위해, 실시예 2.3의 방법을 이용하여 각막 형태변화를 확인하였다. 감초 추출물의 투여량은 JGT-250(250mg/kg 투여) 기준 감초의 투여량인 84mg/kg을 투여하였다.In order to confirm the therapeutic effect of dry eye extract of licorice root extract, the change of corneal morphology was confirmed by the method of Example 2.3. The dose of licorice extract was 84 mg / kg, the dose of Licorice based on JGT-250 (250 mg / kg dose).
그 결과, 도 6에 나타낸 바와 같이, 정상군과 비교하여 감초 추출물 투여군의 각막형태가 뚜렷하지 않은 것을 확인할 수 있으며, 점수로 평가한 경우에도 안구건조증 질환군과 비교하여 감초 추출물 투여군의 각막 형태변화 점수가 유의적인 감소를 보이지 않아 감초 추출물이 각막 형태변화를 억제하지 못하는 것을 확인하였다.As a result, as shown in FIG. 6, it was confirmed that the corneal morphology of the licorice extract-treated group was not clear compared to the normal group, and the corneal morphology of the licorice extract-treated group No significant decrease in the score was observed, indicating that the licorice extract did not inhibit corneal morphological changes.
따라서, 상기 비교예를 통해 감초 추출물 단독으로는 눈물분비량에 영향을 주지 않으며, 각막 형태변화 억제 효과를 가지지 않아 안구건조증 치료용 조성물로는 부적합한 것을 확인하였다.Thus, it was confirmed that the licorice extract alone did not affect the amount of tear secretion, and had no effect of inhibiting the corneal morphological change. Thus, it was confirmed that it was not suitable as a composition for treating dry eye syndrome.
본 명세서는 본 발명의 기술 분야에서 통상의 지식을 가진 자이면 충분히 인식하고 유추할 수 있는 내용은 그 상세한 기재를 생략하였으며, 본 명세서에 기재된 구체적인 예시들 이외에 본 발명의 기술적 사상이나 필수적 구성을 변경하지 않는 범위내에서 보다 다양한 변형이 가능하다. 따라서 본 발명은 본 명세서에서 구체적으로 설명하고 예시한 것과 다른 방식으로 실시될 수 있으며, 이는 본 발명의 기술 분야에 통상의 지식을 가진 자이면 이해할 수 있는 사항이다.It is to be understood that both the foregoing general description and the following detailed description of the present invention are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. More variations are possible within a range that does not. Accordingly, the present invention may be embodied in other ways than those specifically described and illustrated herein, and it may be understood by those skilled in the art.
Claims (12)
- 작약 및 감초의 혼합추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 치료용 약학적 조성물.A pharmaceutical composition for preventing or treating dry eye syndrome, which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- 제1항에 있어서,The method according to claim 1,상기 추출물은 물, 탄소수 1 내지 4의 저급 알코올 및 이들의 혼합 용매로 구성되는 군으로부터 선택되는 용매로 추출한 추출물인 것인, 약학적 조성물Wherein the extract is an extract extracted with a solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof.
- 제1항에 있어서,The method according to claim 1,상기 분획물은 상기 혼합 추출물의 n-헥산 분획물, 에틸 아세테이트 분획물, 부탄올 분획물 또는 물 분획물인 것인, 약학적 조성물.Wherein said fraction is an n-hexane fraction, an ethyl acetate fraction, a butanol fraction or a water fraction of said mixed extract.
- 제1항에 있어서,The method according to claim 1,상기 추출물은 감압고온추출, 열탕추출, 환류추출, 열수추출, 상온추출, 초음파 추출 또는 증기추출 방법으로 추출한 것을 특징으로 하는 안구건조증 예방 및 치료용 약학적 조성물.The pharmaceutical composition for preventing and treating dry eye syndrome according to claim 1, wherein the extract is extracted by a method selected from the group consisting of vacuum, high temperature extraction, hot water extraction, reflux extraction, hot water extraction, room temperature extraction, ultrasonic extraction or steam extraction.
- 제1항에 있어서,The method according to claim 1,상기 조성물은 눈물 분비량을 증가시키거나 각막 형태 변화를 억제하거나, 눈피로를 개선하는 것인, 약학적 조성물.Wherein said composition increases tear secretion, inhibits corneal morphological changes, or improves eye fatigue.
- 제1항에 있어서,The method according to claim 1,상기 조성물은 작약 및 감초를 10:1 내지 1:10 중량비율로 혼합하는 것을 특징으로 하는, 약학적 조성물.Wherein said composition is a mixture of peony root and licorice root in a weight ratio of 10: 1 to 1:10.
- 제1항에 있어서, 상기 약학적 조성물은 안구 외용제형인 것인, 약학적 조성물.The pharmaceutical composition according to claim 1, wherein the pharmaceutical composition is an external preparation for eyeball.
- 작약 및 감초의 혼합추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증 예방 또는 개선용 건강기능식품.A health functional food for preventing or ameliorating dry eye syndrome, which comprises a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- 작약 및 감초의 혼합추출물 또는 이의 분획물을 유효성분으로 포함하는 눈물 분비량 증진용 건강기능식품.A health functional food for enhancing tear secretion content comprising a mixture of peony and licorice or a fraction thereof as an active ingredient.
- 작약 및 감초의 혼합추출물 또는 이의 분획물을 개체에 투여하는 단계를 포함하는 안구건조증 예방 또는 치료방법.A method for preventing or treating dry eye syndrome comprising administering to a subject a mixed extract of peony root and licorice or a fraction thereof.
- 작약 및 감초의 혼합추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증의 예방 또는 개선용 눈 화장료 첨가제 조성물. A cosmetic additive composition for preventing or ameliorating dry eye syndrome comprising a mixed extract of peony root and licorice or a fraction thereof as an active ingredient.
- 작약 및 감초의 혼합추출물 또는 이의 분획물을 유효성분으로 포함하는 안구건조증의 예방 또는 치료용 의약외품 조성물.A quasi-drug composition for the prevention or treatment of dry eye syndrome comprising a mixture of peony root and licorice as an active ingredient or a fraction thereof.
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US20040058015A1 (en) * | 2000-06-01 | 2004-03-25 | Yuanjin Tao | Compositions and methods for treating eye discomfort and eye disease |
CN102210821A (en) * | 2011-05-15 | 2011-10-12 | 王春兰 | Traditional Chinese medicine for treating xerophthalmia |
CN104383296A (en) * | 2014-12-04 | 2015-03-04 | 湖南中医药大学 | Traditional Chinese medicine composition for treating xerophthalmia and preparation method of traditional Chinese medicine composition for treating xerophthalmia |
CN104435685A (en) * | 2014-11-30 | 2015-03-25 | 顾蔷怡 | Medicine for treating xerophthalmia and preparation method thereof |
KR20170004245A (en) * | 2015-07-01 | 2017-01-11 | 김무연 | Pharmaceutical composition for preventing or treating ocular diseases |
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US20040058015A1 (en) * | 2000-06-01 | 2004-03-25 | Yuanjin Tao | Compositions and methods for treating eye discomfort and eye disease |
CN102210821A (en) * | 2011-05-15 | 2011-10-12 | 王春兰 | Traditional Chinese medicine for treating xerophthalmia |
CN104435685A (en) * | 2014-11-30 | 2015-03-25 | 顾蔷怡 | Medicine for treating xerophthalmia and preparation method thereof |
CN104383296A (en) * | 2014-12-04 | 2015-03-04 | 湖南中医药大学 | Traditional Chinese medicine composition for treating xerophthalmia and preparation method of traditional Chinese medicine composition for treating xerophthalmia |
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