WO2019121911A1 - Appareil et procédé de création d'ondes stationnaires résonantes dans un tissu biologique - Google Patents

Appareil et procédé de création d'ondes stationnaires résonantes dans un tissu biologique Download PDF

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Publication number
WO2019121911A1
WO2019121911A1 PCT/EP2018/085839 EP2018085839W WO2019121911A1 WO 2019121911 A1 WO2019121911 A1 WO 2019121911A1 EP 2018085839 W EP2018085839 W EP 2018085839W WO 2019121911 A1 WO2019121911 A1 WO 2019121911A1
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Prior art keywords
seconds
modulated
frequency
carrier signals
mhz
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PCT/EP2018/085839
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English (en)
Inventor
Albert Nunez
Eric Miller
Gary MINKER
Alex Nunez
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Innovarius Ltd.
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Publication of WO2019121911A1 publication Critical patent/WO2019121911A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/06—Electrodes for high-frequency therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/36014—External stimulators, e.g. with patch electrodes
    • A61N1/3603—Control systems
    • A61N1/36034—Control systems specified by the stimulation parameters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/40—Applying electric fields by inductive or capacitive coupling ; Applying radio-frequency signals
    • A61N1/403—Applying electric fields by inductive or capacitive coupling ; Applying radio-frequency signals for thermotherapy, e.g. hyperthermia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N7/00—Ultrasound therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0408—Use-related aspects
    • A61N1/0464—Specially adapted for promoting tissue growth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0408—Use-related aspects
    • A61N1/0468—Specially adapted for promoting wound healing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/36002—Cancer treatment, e.g. tumour
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/36014—External stimulators, e.g. with patch electrodes
    • A61N1/36021—External stimulators, e.g. with patch electrodes for treatment of pain
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/36014—External stimulators, e.g. with patch electrodes
    • A61N1/36025—External stimulators, e.g. with patch electrodes for treating a mental or cerebral condition

Definitions

  • the present invention relates to an apparatus for and a method of creating resonant standing waves in biological tissue, in particular in the treatment of medical conditions.
  • the present invention has application in acting to energize biological tissue to increase the potential energy of water in the tissue in vivo, which is postulated to separate charges of water into positive water ions outside of a negative EZ water layer that forms along hydrophilic membranes in a region of the tissue, and also in enhancing protein unfolding in the tissue.
  • the present invention provides an apparatus for creating resonant standing waves in biological tissue, in particular in the treatment of medical conditions, comprising at least one applicator for application to biological tissue and a drive unit for driving the at least one applicator simultaneously with AM modulated and FM modulated carrier signals in order to create resonant standing waves in the tissue.
  • the apparatus comprises a single applicator.
  • the apparatus comprises a plurality of applicators.
  • the applicators are arranged in one or more arrays.
  • the least one applicator comprises a director layer which includes coupling slots, a Z-patch layer which includes a Z-patch and overlies the director layer, and a reflector layer which includes a patterned reflector for defining the polarity ratio.
  • the or each AM modulated carrier signal has a plurality of AM modulation frequencies of changing frequency, as an AM modulation composition set, applied thereto in succession for respective periods of time.
  • a plurality of FM modulated carrier signals are applied simultaneously to the at least one applicator.
  • the at least one applicator has three different FM modulated carrier signals applied thereto, as a triplet.
  • one or more of the FM modulated carrier signals have a plurality of FM modulation frequencies of changing frequency, as an FM modulation composition set, applied thereto in succession for respective periods of time.
  • one or more of the carrier signals are square-wave signals.
  • one or more of the carrier signals are triangular-wave signals.
  • one or more of the carrier signals are sinusoidal-wave signals.
  • the frequency of one or more of the carrier signals is from about 5 MHz to about 13 MHz.
  • the frequency of the one or more carrier signals is from about 100 MHz to about 500 MHz.
  • the frequency of the one or more carrier signals is from about 2.3 GHz to about 3.2 GHz. In one embodiment the one or more carrier signals have a center frequency of about 434 MHz.
  • the or each AM modulated carrier signal is modulated between a first, low-power state and a second, high-power state, thereby allowing control of power level.
  • the or each AM modulated carrier signal is modulated with an applied frequency of from about 1 Hz to about 2.6 MHz, optionally about 1 Hz to about 1 MHz.
  • the or each FM modulated carrier signal is modulated with an applied frequency of from about 1 Hz to about 50 kHz MHz, optionally about 1 Hz to about 10 kHz, optionally about 1 Hz to about 1 kHz.
  • the present invention provides an apparatus for or method of creating resonant standing waves in biological tissue of a subject in treating spinal intervertebral disc disease (IVDD) or musculo-skeletal disorders, pain management and/or tissue regeneration, comprising applying AM modulated and FM modulated carrier signals simultaneously to a subject in order to create resonant standing waves in the tissue.
  • IVDD spinal intervertebral disc disease
  • musculo-skeletal disorders pain management and/or tissue regeneration
  • the present invention provides an apparatus for or method of creating resonant standing waves in biological tissue of a subject in treating anti-inflammatory and/or neurological disorders, comprising applying AM modulated and FM modulated carrier signals simultaneously to a subject in order to create resonant standing waves in the tissue.
  • the present invention provides an apparatus for or method of creating resonant standing waves in biological tissue of a subject in treating oncological disorders, in particular tumors, comprising applying AM modulated and FM modulated carrier signals simultaneously to a subject in order to create resonant standing waves in the tissue.
  • the present invention provides an apparatus for or method of creating resonant standing waves in biological tissue of a subject in treating mastitis, in particular bovine mastitis, comprising applying AM modulated and FM modulated carrier signals simultaneously to a subject in order to create resonant standing waves in the tissue.
  • the present invention provides an apparatus for or method of creating resonant standing waves in biological tissue of a subject in wound and/or bone healing, comprising applying AM modulated and FM modulated carrier signals simultaneously to a subject in order to create resonant standing waves in the tissue.
  • the present invention provides an apparatus for or method of creating resonant standing waves in biological tissue of a subject in treating body stones or calcifications, in particular renal and bladder stones, comprising applying AM modulated and FM modulated carrier signals simultaneously to a subject in order to create resonant standing waves in the tissue.
  • the AM modulated and FM modulated carrier signals are applied through a single applicator.
  • the AM modulated and FM modulated carrier signals are applied through a plurality of applicators.
  • the applicators are arranged in one or more arrays. In one embodiment a single AM modulated carrier signal is applied to the subject.
  • the or each AM modulated carrier signal has a plurality of AM modulation frequencies of changing frequency, as an AM modulation composition set, applied thereto in succession for respective periods of time.
  • a plurality of FM modulated carrier signals are applied simultaneously to the subject.
  • three different FM modulated carrier signals are applied simultaneously to the subject, as a triplet.
  • one or more of the FM modulated carrier signals have a plurality of FM modulation frequencies of changing frequency, as an FM modulation composition set, applied thereto in succession for respective periods of time.
  • one or more of the carrier signals are square-wave signals.
  • one or more of the carrier signals are triangular-wave signals.
  • one or more of the carrier signals are sinusoidal-wave signals.
  • the frequency of one or more of the carrier signals is from about 5 MHz to about 13 MHz.
  • the frequency of the one or more carrier signals is from about 100 MHz to about 500 MHz. In one embodiment the frequency of the one or more carrier signals is from about 2.3 GHz to about 3.2 GHz.
  • the one or more carrier signals have a center frequency of about 434 MHz.
  • the or each AM modulated carrier signal is modulated between a first, low-power state and a second, high-power state, thereby allowing control of power level.
  • the or each AM modulated carrier signal is modulated with an applied frequency of from about 1 Hz to about 2.6 MHz, optionally about 1 Hz to about 1 MHz.
  • the or each FM modulated carrier signal is modulated with an applied frequency of from about 1 Hz to about 50 kHz MHz, optionally about 1 Hz to about 10 kHz, optionally about 1 Hz to about 1 kHz.
  • Figure 1 illustrates an apparatus in accordance with a first embodiment of the present invention
  • Figure 2 illustrates an apparatus in accordance with a second embodiment of the present invention
  • Figure 3 illustrates an apparatus in accordance with a third embodiment of the present invention
  • Figure 4 illustrates an apparatus in accordance with a fourth embodiment of the present invention
  • FIGS 5(a) and (b) illustrate an applicator in accordance with one embodiment of the present invention for use in the apparatus of the present invention
  • Figure 6 illustrates one exemplary modulation for the AM modulated carrier signal in accordance with an embodiment of the present invention
  • Figure 7 illustrates a plot of nerve blocking and pain relief scores in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 8 illustrates images of the thixotropic and thermal rise in muscle phantom following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 9 illustrates images of the thixotropic and thermal rise in muscle phantom following application of AM and FM modulations in repetition in accordance with another embodiment of the present invention.
  • Figure 10 illustrates a plot of neurological rehabilitation scores in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 11 illustrates a plot of pain scores in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 12 illustrates images showing the epithelialization of the hard palate of a subject following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 13 illustrates a plot of wound healing in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 14 illustrates a plot of healing of bone fractures in pediatric subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 15 illustrates a plot of healing of bone fractures in adult subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 16 illustrates images of the dissolution of bladder stones in a subject following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 17 illustrates a plot of visual progress scores in the dissolution of bladder stones in the subject of Figure 16;
  • Figure 18 illustrates a plot of effectiveness scores in treating body stones in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 19 illustrates a plot of respiratory distress scores in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention. - g .
  • Figure 20 illustrates a plot of effectiveness scores in cardiac and circulatory improvement in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 21 illustrates a plot of disease activity index (CDAI) in subjects suffering from Crohn's disease following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • FIGS 22 and 23 illustrate respectively plots of Cincinnati orthopedic disability index (CODI) and pain scores in subjects suffering from arthritis following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 24 illustrates a plot of insulin requirement in subjects following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figure 25 illustrates a plot of pain scores in subjects suffering from sports injuries following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention
  • Figures 26 and 27 illustrate respectively plots of muscle soreness scores and pain scores in subjects suffering from muscular conditions following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention.
  • Figure 28 illustrates a plot of effectiveness scores in reducing tremors in subjects suffering from tremors following application of AM and FM modulations in repetition in accordance with one embodiment of the present invention.
  • the apparatus comprises at least one applicator 103 for application to biological tissue T and a drive unit 105 for driving the at least one applicator 103 simultaneously with AM modulated and FM modulated carrier signals in order to create resonant standing waves in the tissue T.
  • the apparatus comprises a single applicator 103.
  • the apparatus comprises a plurality of applicators 103.
  • the applicators 103a-d are arranged in one or more arrays 107.
  • each applicator 103 comprises a first, facing dielectric layer 121 which is applied to the tissue T, a second, director layer 123 which includes coupling slots 125 and overlies the first layer 121, a third, spacing dielectric layer 127 which overlies the second layer 123, a fourth, Z-patch layer 129 which includes a Z-patch 131 and overlies the third layer 127, a fifth, spacing dielectric layer 133 which overlies the fourth layer 129, a sixth, reflector layer 135 which includes a patterned reflector 137 for defining the polarity ratio, and a seventh, absorbing dielectric layer 139 which overlies the sixth layer 135 and provides an electromagnetic (EM) shield.
  • EM electromagnetic
  • the or each AM modulated carrier signal has a plurality of AM modulation frequencies of changing frequency, as an AM modulation composition set, applied thereto in succession for respective periods of time.
  • a plurality of FM modulated carrier signals are applied simultaneously to the at least one applicator 103.
  • one or more of the FM modulated carrier signals have a plurality of FM modulation frequencies of changing frequency, as an FM modulation composition set, applied thereto in succession for respective periods of time.
  • the at least one applicator 103 has three different FM modulated carrier signals applied thereto, as a triplet.
  • one or more of the FM modulated carrier signals have a single FM modulation frequency applied thereto continuously.
  • one or more of the carrier signals are square-wave signals.
  • one or more of the carrier signals are triangular-wave signals.
  • one or more of the carrier signals are sinusoidal-wave signals.
  • the frequency of one or more of the carrier signals is from about 5 MHz to about 13 MHz.
  • the frequency of the one or more carrier signals is from about 100 MHz to about 500 MHz.
  • the frequency of the one or more carrier signals is from about 2.3 GHz to about 3.2 GHz.
  • the one or more carrier signals have a center frequency of 434 MHz, with control of AM and FM modulation, power level and duty cycle.
  • AM modulation allows for power of the associated carrier wave to be modulated between a first, low-power state and a second, high- power state, thereby allowing control of the power level.
  • the AM modulated carrier signal can be modulated between a 4 W always-on power state to a 27 W peak power state, with an average power of 11.5 W, as illustrated in Figure 6.
  • the or each AM modulated carrier signal is modulated with an applied frequency of from about 0.1 MHz to about 2.6 MHz.
  • the one or more FM modulated carrier signals are modulated with applied frequencies in the range of from about 0.1 MHz to about 50000 Hz using an audio digital signal.
  • wave shaping of each carrier signal is achieved, and a series of resonant standing wave configurations can be applied to impart potential energy into water in the biological tissue by manipulation of the water micro-tubular cellular interface, being tuned to match the resonant frequencies of various targeted tissue types, such as organs and glands.
  • the carrier signals can be pulsated to achieve specific physiological rhythmic effects arising from the effects of standing wave generation and the resonant frequency stimulation of the biological tissue, which are soft glassy materials in kind [Fredburg et at, "The cytoskeleton as a soft glassy material”, Cytoskeletal Mechanics: Models and Measurements, pages 3 to 8 (2006)].
  • the apparatus is capable of applying a wide range of resonant arrangements that deliver clinically-relevant, non-invasive therapies with the purpose of providing electronic, anti-inflammatory and other physiological effects.
  • the apparatus is controlled continuously to operate to generate standing waves in biological tissue while operating within a predetermined range of temperature, for example, a temperature ceiling of from about 37 °C to about 41 °C.
  • the power and duty cycle of the AM and FM modulated carrier signals are controlled in order to maintain continuous application of the carrier signals, and so prevent the apparatus from turning off when a target temperature is approached.
  • the power and duty cycle of the AM and FM modulated carrier signals are controlled to maintain the desired temperature within +/- 0.1 °C.
  • the apparatus successively applies a test series of different resonant frequencies to the carrier signals for a predetermined period of time, for example, one minute, and measures the time taken to raise the temperature of the tissue with each specific frequency set, and this time is compared to the temperature rise for each respective frequency set. After this series of test frequency runs, the apparatus selects the most efficient resonant frequency set as determined by the fastest temperature rise in a given amount of time in order to continue the therapy with the ideal resonant frequency for the tissue being treated.
  • multiple applicators 103 are made to play in harmony by offsetting each set of applicators 103 in Fibonacci sequence, or so-called golden "Phi", in order to create a harmonic ratio between all of applicators 103 applied over the area of the body to be energized.
  • the base frequency of this set is 1.618 Hz and the applicators 103 are driven for a predetermined period of time, for example, from about 1 second to about 10 seconds, then the base frequency is raised by 1.618 Hz, with the applicators again being driven for a predetermined period of time, for example, from about 1 second to about 10 seconds, and this incremental increase in the base frequency is repeated in order to produce an ascending set of harmonic standing waves that efficiently oscillate the tissue.
  • the base frequency can be made to climb up to a predetermined frequency, for example, about 13000 Hz or about 2.6 MHz.
  • the ascending harmonic is repeated.
  • a plurality of FM modulated carrier signals are applied simultaneously over an AM modulated carrier signal.
  • each FM modulated carrier signal can be different over each of a plurality of ranges of frequency.
  • the FM modulated carrier signals can be made to modulate at a first, slow rate at a frequency of less than from about 1 Hz to about 10 Hz, for example, at 1 Hz or 7.83 Hz.
  • the FM modulated carrier signals are made to be offset by a 1.618033 ratio in Fibonacci sequence to each other in order to form a harmonic standing wave that will ascend by increasing the base frequency by 1.618 Hz, and applied for a predetermined period of time, for example, from about 1 second to about 10 seconds.
  • the base frequency can be made to climb up to a predetermined frequency, for example, about 20000 Hz.
  • the ascending harmonic is repeated.
  • the FM modulated carrier signals may be offset by from about 0 Hz to about 3 Hz, from about 5 Hz to about 8 Hz, from about 8 Hz to about 13 Hz and from about 13 Hz to about 33 Hz in order to create an oscillatory effect such as experienced with binaural beats.
  • one audio signal can applied at 174 Hz and the other two audio signals can be applied at 528 Hz and 529.6 Hz.
  • This arrangement provides a low-frequency harmonic drone with a binaural pulsing of 2.6 Hz between the 528 Hz and 529.6 Hz frequencies.
  • the net effect is to augment the energy delivered to the target area with a binaural rhythmic stimulation at 2.6 Hz, which can be used to entrain the brain with waves of any desired frequency as user specified when placed over the cerebrum or other parts of the brain.
  • the energy transferred by the standing waves generated by the one or more applicators 103 in the tissue is used to increase the potential energy of the water inside of the mitochondria in the tissue under the applicator.
  • This energy can convert ADP into ATP and NAD into NADH through the conversion of ionic standing wave oscillation of the water molecules inside of the mitochondria and tissues into potential energy for charge separation and the creation of ordered structured water along hydrophilic surfaces.
  • the apparatus produces standing waves in tissues that are powerful stimulators of mechanotransduction in vivo, which influence integrin dynamics and cause force-dependent effects such as endothelial responses to shear stress and the regulation of growth and gene expression in many tissues through forced oscillations.
  • the apparatus augments the electromagnetic stimulation of biological tissue through resonance in order to stimulate the tissue into the production of heat shock protein (HSP), such as gp96, hsp70 and hsp90 in vivo.
  • HSP heat shock protein
  • HSPs have been disclosed, for example, in US-A-2003/0012793, positively to effect biological tissue specifically with regards to the rehabilitation of neurologic tissue, wounds, fractures, organs such as the heart, lungs, liver, spleen, kidneys, stomach, small and large intestines, prostate, testes, ovaries, adrenal glands, thyroid glands, pituitary glands, brain, eyes, spinal cord, peripheral nerves, tendons, ligaments, cartilage, bones, hair, nails and skin.
  • the apparatus can be utilized to perform local ultra-high frequency resonant hyperthermia, in order to stimulate donor sites for cancer vaccines.
  • donor sites are prepared for a predetermined period of time, for example, from about 30 minutes to about 60 minutes, prior to vaccine delivery intradermally to prepare dendritic cells for immunization.
  • the apparatus can be utilized to perform local ultra-high frequency resonant hyperthermia of solid tumors alone or in combination with supraparamagnetic iron oxide nanoparticles (SPIONs) of various sizes and in metamaterial combinations of, for example, from about 5 nm to about 30 nm, carboplatin, cisplatin, 2-desoxy-D-glucose and dl-glyceraldehyde, and other chemotherapeutic agents.
  • SPIONs supraparamagnetic iron oxide nanoparticles
  • LTH Localized tumor hyperthermia
  • cyto protection can be offered by electromagnetic (EM) field induction of stress proteins [Carmody et al, Journal of Cellular Biochemistry, 79(3), pages 453-459 (2000)].
  • Stimulation of human promyelocytic HL60 cells by a magnetic field at normal growth temperatures has been disclosed to result in heat shock factor 1 activation and heat shock element binding, a sequence of events that mediates the stress-induced transcription of the stress gene hsp70 and increased synthesis of the stress response protein hsp70kD [Lin et al, Electromagnetic field exposure induces rapid, transitory heat shock factor activation in human cells, J. Cell. Biochem., 66, pages 482-488, 1997].
  • Nerve conduction block induced by high-frequency biphasic rectangular pulses has been disclosed [Zhang et a/, IEEE Transactions on Biomedical Engineering, 53(7), pages 1433-1436 (2006)].
  • axons of different diameters (2-20 pm) can be blocked completely at supra-threshold intensities when the stimulation frequency is above 10 kHz.
  • hsp70 70 kDa HSP
  • hsp27 27 kDa HSP
  • constitutive expression of hsp27 is limited to many (but not all) sensory and motor neurons of the brain stem and spinal cord, while there is little or no constitutive expression of hsp70.
  • inducible expression of both hsp70 and hsp27 is present in many areas of the brain and retina and is associated with cellular resistance to a variety of insults.
  • the apparatus can be employed as a method to induce hsp70 and hsp27 expression non-invasively in neurological tissue. Ponderomotive Force
  • the apparatus is employed to apply a ponderomotive force, being a non-linear force that a charged particle experiences in an inhomogeneous oscillating electromagnetic field, locally over a biological or non-biological material in order to lower the density of the material while the apparatus is stimulating the target material.
  • the apparatus is tuned to biological tissues and the ponderomotive force is employed to decrease the density and viscosity of the same tissues, which increases the thixotropic rate of thick gels or fluids under the at least one applicator 103 for the purpose of biological stimulation.
  • the biologic soft glassy materials under the at least one applicator 103 are stimulated and oscillated by the standing waves it allows for the "rejuvenation” (uniform agitation and distribution) of these materials and facilitates the removal of intracellular debris and toxins from them non-invasively.
  • the apparatus can be used to stimulate and rejuvenate soft glassy materials such as biological tissue with oscillating standing waves of various AM modulations and one or more FM modulated carrier signals.
  • Modulated stimulation generated by audio-to-digital sound generators increases the oscillation and agitation of biological soft glassy materials and promotes the thixotropic properties of the soft glassy material composed of water, microtubules, membrane bilayers, proteins and intracellular organelles, cells in general as stimulated locally.
  • the standing waves generated by the apparatus affect the thixotropic properties of water especially along biological and other polymer gels. Water along hydrophilic surfaces, micro-tubular networks and intracellular water tend to gel and stiffen up over time, as well as become contaminated with metabolic debris, waste products and toxins.
  • the cyclotron ionic stimulation and rhythmic standing wave oscillation of the water and biological tissues generated under the at least one applicator 103 are made mechanically to vibrate the soft glassy material that is the intracellular milieu and cause the soft glassy material that is the cells, tissue and water to become thixotropic. That is, the gelled ordered structured water begins to become thin and less viscous over time when shaken and oscillated by the AM modulation and FM modulated standing waves generated by the apparatus.
  • the biologic soft glassy materials under the applicator 103 are stimulated and oscillated by the standing waves, the stimulation allows for the "rejuvenation" (uniform agitation and distribution) of these materials and facilitates the removal of intracellular debris and toxins from them non-invasively.
  • the potential energy of vibration is used by the water in the cells to create ordered structured water and to facilitate the separation of charges along hydrophilic surfaces into negative charges close to the hydrophilic surface, with positive charges just outside the negative EZ Water layer zone formed along the hydrophilic surface.
  • Figure 8 illustrates the thixotropic and thermal rise demonstrated in muscle phantom. With external application of an applicator to the tank, the area of thixotropic response extends for greater than 15 cm with 15 minutes of therapy.
  • a one gallon milk jug was filled with cold load phantom gel (dimensions: 9 3 ⁇ 4 inches high, and a square base of 5 3 ⁇ 4 inches by 5 3 ⁇ 4 inches).
  • An applicator pad was placed on the bottom of the jug for one hour.
  • the initial temperature was 70 °F and a maximum temperature of 101 °F was reached after over an hour. The heat completely penetrated throughout the whole milk jug.
  • Figure 9 illustrates the thixotropic and thermal rise demonstrated in the phantom.
  • the apparatus creates standing waves in tissue to decrease local edema of the tissue by oscillating the edematous tissue and forcing a phase change of water from liquid H 2 0 into liquid crystal H 3 0 2 , known as EZ water, along hydrophilic surfaces.
  • the oscillation increases the potential energy of the edematous water found in the tissues and forces the same to separate charges into positive and negative along hydrophilic surfaces and to assemble into ordered structured EZ water along the same hydrophilic surfaces.
  • the effect is a decrease in edema in the tissues under the applicator.
  • the at least one applicator 103 can be used as a means to deliver a neurological set of standing waves for the specific intention of decreasing edema, increasing blood flow and oxygenation of neurological tissues and to enhance the production of HSPs in situ local regionally under the at least one applicator 103.
  • the apparatus is used to create standing waves and electromagnetic stimulation of neurological tissue such as the brain, spinal cord and peripheral nerves in vivo, with the or each applicator 103 being positioned over the brain in order to stimulate the brain with standing waves having a frequency of from about 3 Hz to about 13 Hz, so producing neuro- protective HSPs in the brain and increasing blood flow and oxygen delivery to the same tissues.
  • the apparatus is used as a method to decrease the severity of seizure disorder and to treat seizure disorder through the stimulation of HSPs.
  • the apparatus is used to treat depression by the stimulation of the left frontal lobe and other areas of the brain.
  • the left frontal lobe is treated for 15 minutes with a target temperature of 37 °C, three times per week for four weeks, and an increase in blood flow and delivery of oxygen to the neurological tissue being stimulated has been demonstrated.
  • the apparatus is used to treat the symptoms of Parkinson's disease by increasing blood flow and oxygen delivery to the cerebellum and by the production of the neuro-protective chemicals hsp70 and hsp27 in the cerebellum and the production of L-Dopa in the Substantia Nigra.
  • BBS B!ood Brain Barrier
  • the apparatus is used as a method to open the blood brain barrier for clinical application.
  • the or each applicator 103 is placed behind the neck and skull of a patient and oscillated with a set of neurologically-enhancing frequencies for a predetermined period of time, for example, from about 20 minutes to about 30 minutes.
  • the apparatus provides for the treatment of Alzheimer's disease by allowing the glial cells to enhance removal of amyloid from neurological tissue, potentially reversing the clinical signs and progression of the disease.
  • the oscillatory effect as disclosed hereinabove, also enhances the removal of intracellular toxins, cellular mediators of inflammation and unwanted protein derived debris from neurological tissue.
  • the apparatus creates standing waves in the brain to entrain the brain with a dominant frequency in the delta, alpha, theta and/or beta brain wave EEG frequency ranges.
  • a frequency of 2.5 Hz in the delta frequency range is employed to entrain the brain into a calm state.
  • a frequency of 7.82 Hz in the theta frequency range is employed to create an alert and awake state.
  • a set of frequencies are applied to one or more applicators placed over an area of neurological injury at periodic intervals for a period of time, for example, for about 20 minutes to about 30 minutes every other day or once weekly for period of about 4 to 6 weeks.
  • HSPs in particular hsp70 and hsp90.
  • hsp70 has been found to be neuro- protective and a promoter of neurological rehabilitation.
  • a series of AM modulated standing square waves are applied in succession which are selected from frequencies of from about 7.82 Hz to about 13 Hz, from about 15 Hz to about 17 Hz, from about 54 Hz to about 56 Hz, about 108 Hz, from about 500 Hz to about 2000 Hz, from about 2000 Hz to about 15000 Hz, from about 15000 Hz to about 1 MHz, and from about 1 MHz to about 2.6 MHz, in combination with FM modulated standing square waves in succession which are selected from frequencies of from about 7.82 Hz to about 13 Hz, from about 15 Hz to about 17 Hz, from about 54 Hz to about 56 Hz, about 108 Hz, and from about 500 Hz to about 528 Hz.
  • Frequencies in the range of about 7.82 Hz to about 13 Hz, and in particular about 10 Hz, can promote neurological rehabilitation.
  • Frequencies in the range of about 16 Hz to about 55 Hz can promote calcium movement in neurologic tissue.
  • the apparatus is used to activate dynamically and reversibly tunable implant structures, such as polymer implants.
  • the implant includes a nanobiological target to be utilized as a manipulative hyperthermia material stimulator.
  • the implant includes a switchable drug release platform by stimulating the implanted or injected target material, for example, a nanolipid ball filled with doxorubicin, with an array of standing waves locally at the target tissue and implanted nanotarget.
  • a switchable drug release platform by stimulating the implanted or injected target material, for example, a nanolipid ball filled with doxorubicin, with an array of standing waves locally at the target tissue and implanted nanotarget.
  • the apparatus comprises frequency compositions which enhance ionic movement within cells, thus enhancing ionic movement and chemical reactions in the area energized by the apparatus.
  • One example of a neurological rehabilitative frequency composition is as follows:
  • each frequency component can be applied from about 3 s to about 10 s.
  • the FM modulation applies a three chord combination at the same time, as follows.
  • this neurological composition was employed in the rehabilitation of dogs from paralysis and paresis due to vertebral disk disease.
  • Target temperatures of between 37 °C and 41 °C were achieved over the area of pathology within 10 to 15 minutes, and stimulation over the area of tissue was maintained for 10 minutes.
  • One example of a neurological rehabilitative frequency composition is as follows:
  • This mode of AM modulation is a temperature-regulated mode.
  • the FM modulation applies a three chord combination at the same time, as follows.
  • Dogs tested for a variety of painful conditions were treated with the above composition. Each dog was given a range of from 2 to 6 treatments, and scores were taken before the first treatment and after the last treatment; the worst possible score being 20 and the best possible score being 0.
  • the apparatus applies the above 2T2 composition in wound repair, with the or each applicator 103 being applied to the wound injury for 20 to 30 minutes every other day or once weekly for 4 to 6 weeks.
  • Frequencies in the range of from 22 to 27 Hz can promote collagen synthesis.
  • a frequency of 108 Hz can provide for treatment of edema.
  • Frequencies in the range of from 500 Hz to 13000 Hz can provide for pain mitigation.
  • a feline patient with eosinophilic granuloma complex of the hard palate showed epithelialization of the hard palate over a three-month period using this composition, as shown in Figure 12.
  • This 18-year old female cat had been treated for four years with depo Medrol monthly injections on presentation.
  • Results for re-epithelialization are over a three month period, 1 hour therapy once weekly for 8 weeks, and then every 2 weeks for one more month. This patient is stable to date.
  • HSR production is attributed to the observed results, as well as multiple mechanisms of action, such as hyperthermia induced vasodilation with its subsequent increase in blood flow, oxygen and plasma protein rich reparative molecules and other humoral regenerative substances.
  • wound rehabilitation frequency composition is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the or each applicator 103 is placed over an area of bone injury or a post-surgical repair site and a composition applied for a predetermined period, for example, from about 20 minutes to about 30 minutes, at periodic intervals, for example, every other day or once weekly, over a period, for example, from about 4 weeks to about 6 weeks.
  • waves of different shape are applied, for example, triangular and square waves.
  • triangular waves having a frequency in the range of from about 31 Hz to about 39 Hz are applied in combination with square waves having a frequency of from about 108 Hz to about 500 Hz in order to promote bone healing and mitigate edema, and from about 10000 Hz to about 50000 Hz in order to provide pain relief.
  • bone repair composition is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the patients were treated once or twice a week. As shown in Figure 15, swelling was reduced by 50% on average after 30 minutes of treatment. A numeric pain score of 1 to 10 was also recorded before and after full treatent. The average decrease in pain scores for patients with the hyperthermia treatment was 7, while the average decrease in pain scores for those patients without the hyperthermia treatment was 4.
  • the apparatus can be employed to shatter, vibrate and dissolve unwanted biological calcifications in tissues non-invasively in vivo, and in particular the non-invasive dissolution of calcific tendinopathy, gall bladder stones, kidney stones, ureteral stones, bladder stones, urethral stones, pancreatic duct stones, sialoliths (salivary duct stones), other stones or calcifications stuck in a duct of the body.
  • the composition is applied periodically, for example, every day or every other day, for a period, for example, from about 30 minutes to about 60 minutes, over the unwanted calcification for a period of time, for example, from about 1 week to about 4 weeks until resolution of the unwanted calcification.
  • the extent of dissolution of calcifications can be monitored with x- ray or ultrasound imaging.
  • a canine with a bladder full of struvite bladder stones was treated with the above composition, and following treatment dissolution of all but one stone occurred, as shown in Figure 16.
  • Treatment was applied for 30 to 60 minutes, three times a week over a three- month period to dissolve the stones.
  • a feline was presented with 11 stones in ureter of about 2 mm to 3 mm in diameter.
  • the feline was given 1 hour of hyperthermia treatment over kidneys and abdomen using the above composition set for 3 days. On the fourth day, a repeat ultrasound showed only 1 stone was left, as shown in Figure 17. Treatments were repeated twice a week for 1 month. It was also noted that the patient went from renal failure to normal.
  • a sample of 3 cats and 5 dogs with stones were treated with the hyperthermia using the above composition set.
  • the patients treated for small stones were treated twice a week for a month.
  • the patients with larger stones or a large number of stones were treated twice a week for 5 to 6 months.
  • the treatment was either ineffective (no decrease in stones), partially effective (20%-50% decrease in stones), effective (50%-90% decrease in stones), or cure (90%-100% decrease in stones).
  • the apparatus can be employed to dissolve and warm unwanted arthritic calcifications in joints of the body and to help regain a normal range of motion when the body is pre-treated with the apparatus and then subjected to rehabilitative stretching.
  • the or each applicator 103 is placed over the desired joint while delivering the unwanted calcification dissolving frequency set for a period of time, for example, about 30 minutes, and then the joint is stretched and flexed with a variety of physical therapy stretching techniques in order to break past areas of restriction with the stretching exercises while the joint is warm and oscillating.
  • treatment of affected joints is periodically, for example, every other day, for a period, for example, 30 minutes, each for a period, for example, four weeks, followed by immediate stretching and range of motion exercises right after each oscillatory treatment.
  • calcification dissolution composition is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the knee was treated with the above composition set of frequencies (Shan4) for 30 minutes with a target temperature of 39 °C. Immediately after the therapy, the swelling was reduced by 60 percent, and, on a pain score scale of 10 (0 being no pain), the pain score was 2 and the range of motion was back to 100 percent.
  • Shan4 composition set of frequencies
  • the therapy was repeated two days later and all of the swelling was gone, motion was at a 100 percent range of motion, and the pain score was 0.
  • a patient was given injectable Polyglycan, a combination of hyaluronic acid, chondroitin 4 and 6 and glucosamine, once to twice weekly for four to six weeks, and the area of interest was energized with the applicator 103 employing a composition set of anti-inflammatory, analgesic collagen regenerative frequencies over the desired joints to be treated at between 37 °C and 41 °C, in order to change the phase of the GAGs (glycosaminoglycans) and the water cartilage interphase at the joint surface.
  • GAGs glycosaminoglycans
  • This treatment is postulated to promote reassembly of a thicker EZ Water and GAG electronegative surface along the joint surface area by promoting a thicker EZ water layer over the joint surface.
  • the charging up of these apposing electronegative joint surfaces increases joint spaces and promotes joint homeostasis.
  • the device is used to enhance a phase transition on the joint surfaces of H 2 0 into H 3 O 2 through a non-invasive dielectric coupling method. This is a particularly effective way to increase electronegativity along a joint surface in a non-invasive way for the treatment of joint inflammation and pain.
  • the apparatus facilitates the removal into extracellular fluid for excretion of excess magnetite (Fe30 4 ), which arises from magnetite crystals from tau proteins in brain microtubules, and other heavy metals from neurologic tissue as found in senile plaques and Alzheimer's disease.
  • the apparatus plays standing waves in a composition that are capable to oscillate the nanoparticles.
  • the apparatus can be employed to stir and agitate the intracellular microtubules and water interface, and facilitate removal of intracellular debris and other mediators of intracellular inflammation and toxins, as intracellular edema, out of the cells.
  • the vibration caused by the various standing wave frequencies in the cells assist in the vibratory removal of intracellular debris into the extracellular environment for efficient removal of this debris out of the cells and out of the body.
  • the oscillation of the water-micro-tubular-cell interface with standing waves of various frequencies created by radio frequency energy of various AM and FM modulations, in particular triplet FM modulations, are used to impart potential energy into the water by means of ionic cyclotron energy, and this gives the water under the influence of the at least one applicator 103 the energy to separate charges into positive and negative charges along hydrophilic surfaces and to create ordered structured water along the same hydrophilic surfaces.
  • variable AM and FM modulated carrier signals imparted into tissues allows an efficient means to stimulate tissues and organs under the at least one applicator 103 with oscillating standing waves capable of removing intracellular debris, toxins and cellular mediators of inflammation out of the cells and nerve cells and axons into the extracellular environment for removal.
  • a thixotropic effect enhanced by magnetophoresis enhances the removal of cellular toxins and mediators of inflammation.
  • the oscillations created in the tissues by the standing waves created by the apparatus offer a non-invasive means to remove intracellular toxins, debris and intracellular mediators of inflammation from inside the cells under the energy delivered by the at least one applicator 103 and the oscillations facilitate the removal of intracellular debris into the extracellular milieu for facilitated excretion.
  • Slower peristaltic AM modulations are alternated with faster intracellular thixotropic oscillations in order to facilitate extracellular pumping of recently excreted/oscillated material from inside of the cell away from the targeted oscillated area for removal.
  • intracellular debris removal composition is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the apparatus can be employed to loosen and break up phlegm in lungs and bronchial and tracheal passages.
  • this composition is used to provide rapid relief of respiratory symptoms by placing the at least one applicator 103 on the dorsal and ventral/cranial and caudal chest wall over the lungs and bronchial bifurcations as species specific for a period of time, for example, from about 15 minutes to about 30 minutes, with a target temperature of from about 37 °C to about 41 °C.
  • Ci rc ulatorv System Su p p o it ( Poo 6 - 2T6)
  • the apparatus can be employed to improve local circulation through the generation of rhythmic AM and FM modulated standing waves that propagate a peristalsis-like wave through tissues in order to facilitate the acceleration of fluid movement through the tissue under the or each applicator 103.
  • the standing wave nanooscillation of cardiac tissue by the apparatus and the standing wave set of cardiac supporting frequencies may be used as a way to deliver increased blood flow and oxygenation to cardiac tissue, induce HSP production and nanothixotropic stimulation of cardiac tissue, and to support nanofluid dynamics in cardiac tissue.
  • composition set of frequencies can be employed to facilitate a 75 bpm pulse rate, with an approximately 1.25 Hz rhythm under the or each applicator 103.
  • a pulse rate monitor provides active feedback of the patient's heart/pulse rate, and the apparatus matches the monitored heart rate as the rhythm employed by the cardiac/circulatory mode of the apparatus. If no active feedback is given by the heart rate monitor, then a 75 beat per minute pulse is delivered as the default.
  • the apparatus can also promote the production of HSPs, including hsp70, hsp90 and hsp27, in cardiac tissue by applying the circulatory set of frequencies as above over the heart or other vessels selected and energized under the or each applicator 103.
  • HSPs including hsp70, hsp90 and hsp27
  • cardiac circulation composition is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the viability of tissues to be transplanted can profit from pre-conditioning by enhancing the synthesis of HSPs with the use of the apparatus.
  • a problem in the field of transplant medicine is the delicate balance of preparing and transporting organ transplants from donors so that organs can be viable for transplant, especially in cases of a prolonged time between organ collection and transplant. This is particularly relevant in the case of lung transplants.
  • the apparatus comprises a chilled, oxygenated, ozonized, fluid media transport chamber, which includes or is lined with a plurality of applicators, typically from two to four applicators 103, in order to impart energy into the chilled fluid environment.
  • the unit may be used as a pre-implant treatment receptacle/vessel for the preparation of the organ, typically a lung sample.
  • the organ can be maintained chilled but energized, for example, by employing at 50% duty cycle, with a composition of frequencies intended to mimic physiologic micro tubular and mitochondrial vibrations into the media during transport in order to optimize the organ sample, in order to nanooscillate the microtubules in the chilled oxygenated media.
  • the organ can be "thawed" by the same device by simply removing or switching off the cooling mechanism.
  • a cooled oxygen and ozone enriched media surrounds the organ during energized transport or standard liquid helium transport in the case of kidneys.
  • the device can be miniaturized and attached to the container or table side ready chamber, and powered by a battery pack or external power supply during transport.
  • Anti -bacteria I anti-fungal and anti-viral resonances can be energized in the oxygenated physiologic fluid milieu bathing the organs in order to help eliminate unwanted pathogens in recipient organs through use of a scheme where organs are preheated and stimulated with anti-pathogenic resonant frequencies.
  • the device can be used to energize an organ while frozen and transported, and then used to thaw the same organ when the time for implantation and thawing occurs.
  • This device may be placed under the organs while in a cooler or freezer, such as by using TMS bulkhead RF connectors into the cooler/freezer to allow energizing of the sample.
  • AM modulation One example of storage and transport composition is as follows: AM modulation :
  • the apparatus can also be used to decrease edema of lungs and other organs for transplant preparation.
  • the apparatus is used to stimulate the fluid around organs in a bath for preparation prior to transplant in order to reduce edema of tissues being prepared for transplant.
  • each applicator 103 can be dressed in a sterile disposable plastic applicator cover and submerged in the bathing physiological solution in order to energize the desired tissue as an electronic anti- inflammatory for organ transplant edema.
  • a water-soluble colloid gel can be mixed with a formulation of radio coupling enhancing minerals selected from at least one of 1-5% concentration of quartz, 1-5% concentration of amethyst, 1-5% concentration of emerald, 1-5% concentration of sapphire, 1-5% concentration of zeolite/clinoptilolite, 1-5% concentration of hematite, 1-5% concentration of snowflake obsidian, 1-5% concentration of magnetite, 1-5% concentration of supraparamagnetic iron oxide nanoparticles (SPIONs).
  • radio coupling enhancing minerals selected from at least one of 1-5% concentration of quartz, 1-5% concentration of amethyst, 1-5% concentration of emerald, 1-5% concentration of sapphire, 1-5% concentration of zeolite/clinoptilolite, 1-5% concentration of hematite, 1-5% concentration of snowflake obsidian, 1-5% concentration of magnetite, 1-5% concentration of supraparamagnetic iron oxide nanoparticles (SPIONs).
  • the radio enhancing minerals can comprise metamaterial supraparamagnetic iron oxide nanoparticulate ferro fluid composition of 30 mg/ml concentration, being a mixture of 20 mg/ml of 30nm SPION and 8 mg/ml of lOnm SPION and 2 mg/ml of 5 nm SPION.
  • the apparatus can be energized over the target area with a frequency composition of from about 44 Hz to about 500 Hz and from about 200 KHz to about 600 KHz, in order to maximize the resonant maximum vibratory rate of the SPION mixture at the target site under the or each applicator 103.
  • a frequency composition of from about 44 Hz to about 500 Hz and from about 200 KHz to about 600 KHz, in order to maximize the resonant maximum vibratory rate of the SPION mixture at the target site under the or each applicator 103.
  • the apparatus can be used to promote the production of HSPs, including hsp70 and hsp90, in intestinal tissue, including the stomach, the small intestine and the large intestine, by applying the circulatory set of frequencies.
  • HSPs including hsp70 and hsp90
  • intestinal tissue including the stomach, the small intestine and the large intestine
  • hsp70 production in the intestine promotes the propagation of beneficial gut flora and is detrimental for unwanted intestinal flora.
  • disease activity indexes (0-21) were recorded.
  • the disease activity index scoring system is shown below in Table 1.
  • the apparatus can be employed to create standing waves in tissue that increase the local temperature under the applicator 103 for the precise control of temperature locally, with considerably lower power as compared to conventional microwave hyperthermia units that require with a water bolus to cool off the skin, by the efficient use of local resonant frequencies in tissues being energized and oscillated by the apparatus, and an efficient back plane on a modified z strip patch meta-material applicator.
  • the apparatus creates standing waves that increase the heat of friction of water and the micro-tubules under the direct contact of the or each applicator 103 to a variable depth range of from about 4 cm to about 15 cm depending on tissue and material treated.
  • AM and FM modulation of the carrier signals allows the temperature of the energized tissues to be raised in a controlled homogenous fashion that lends itself well to local hyperthermia of biological tissues with a wide margin of safety.
  • the apparatus can be employed to enhance apoptosis of neoplastic cells by matching the resonant frequency of those neoplastic cells, and one or more of increasing the local temperature of the tumor, increasing the local pH, increasing local oxygen delivery to the tumor, increasing mitochondrial damage of tumor cells, increasing intra-tumor production of tissue necrotic factor alpha, activating the immune system against the tumor through the production of TNF alpha and hsp70, and promoting free radical damage inside the tumor.
  • the apparatus can also be employed to facilitate the presentation of oscillated and heated tumor parts to the dendritic cells, and stimulate an immune response with the purpose of creating an in vivo cancer vaccine that specifically targets the tumor being warmed and oscillated under the or each applicator 103.
  • the apparatus can also be used in combination with supraparamagnetic iron oxide nanoparticles (SPIONs), gold nanoshell supraparamagnetic iron oxide nanoparticles (gold SPIONs), chemotherapeutic drugs, such as carboplatin, cisplatin, 2-desoxy-D-glucose, dl-glycerol-aldehyde, immune therapy and glucose metabolic inhibitors, in order to further increase metabolic stress inside the tumors and enhance an activated immune response against the tumor in vivo.
  • SPIONs supraparamagnetic iron oxide nanoparticles
  • gold SPIONs gold nanoshell supraparamagnetic iron oxide nanoparticles
  • chemotherapeutic drugs such as carboplatin, cisplatin, 2-desoxy-D-glucose, dl-glycerol-aldehyde, immune therapy and glucose metabolic inhibitors, in order to further increase metabolic stress inside the tumors and enhance an activated immune response against the tumor in vivo.
  • the composition set can include frequencies which reduce cancer breakthrough pain by oscillating out chemical mediators of inflammation from around pain receptors surrounding the tumor and by providing pain mitigating frequency compositions that include pain blocking frequencies, for example, frequencies in the range of from about 10000 Hz to about 50000 Hz.
  • cancer killing composition is as follows:
  • This set of frequencies is effective to facilitate the removal of intracellular debris and promote apoptosis of bacteria, viruses, fungi and neoplastic cells, and, when used in combination with supraparamagnetic iron oxide solutions (SPIOSMs), as a way to inactivate various bacterial and fungal biofilms and as a method to enhance heating of infected tissues.
  • SPIOSMs supraparamagnetic iron oxide solutions
  • the apparatus can be employed to stimulate cell propagation and support stem cell activation and differentiation in tissues, in combination with stimulating the production of hsp70.
  • composition set for the stimulation and differentiation of osteogenic stem cells and fat heating is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the present invention has particular application in treating Spinal intervertebral disc disease (IVDD).
  • IVDD Spinal intervertebral disc disease
  • the apparatus can be used for stem cell stimulation using an externally-applied applicator 103 on stem cell donor sites, including the falciform ligament and the sternum, ileac crest and femurs.
  • a patient was treated for 30 minutes at a target temperature of about 37 °C using a composition of stimulatory and peristaltic-like resonant frequencies for the activation of the donor sites, and then subsequently a composition set of secretory rhythms were pulsated for 5 minutes at 1.25 Hz for 8 seconds and 3.0 Hz for 5 seconds, which is repeated for 5 minutes.
  • the overall hierarchical pattern of peristaltic-like distribution of the applied signals is applied to the applicators 103 with reference to the patient's orientation, such that the waves move from the donor site to the recipient site and is the stimulated at the recipient site for a defined period, typically from about 10 minutes to about 30 minutes per site after an initial period, typically 5 minutes, of peristaltic tractor waves moving towards the donor site.
  • Insulin Product i o n Insulin Product i o n (Faiva 9 )
  • the apparatus can be employed to stimulate insulin production and support hsp70 activation in pancreatic tissue in the presence of natural iron oxide or administered iron oxide nanoparticles.
  • One example of an insulin production composition set is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the apparatus can be employed in the short-term management of musculoskeletal and sport injuries.
  • This frequency set can stimulate repair processes, increase drug activity, allow more efficient relief from pain, help in the removal of toxic wastes, increase tendon extensibility and reduce muscle and joint stiffness.
  • Hyperthermia induced vasodilation secondary to these specific standing waves induces hyperemia, improves local tissue drainage, increases metabolic rate and induces alterations in the cell membrane to facilitate the removal of lactic acid and other toxic wastes.
  • musculo-skeletal composition set is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • a muscle soreness numeric score was recorded before treatment and the day after treatment for the human patients, with 0 representing no soreness and 10 representing extreme soreness. As will be seen from Figure 26, a marked reduction in soreness was observed, with most having no soreness after treatment.
  • the apparatus can be employed in the management of neuromuscular disease, including Parkinson's disease like tremors.
  • the frequencies are designed to be neuro supportive.
  • neuromuscular supportive composition set is as follows:
  • the FM modulation applies a three chord combination at the same time, as follows.
  • the apparatus comprises a plurality of applicators 103, for example, from 2 to 4 applicators 103 or from 4 to 8 applicators 103.
  • the applicators 103 can be controlled individually or in one or more groups.
  • This configuration allows multiple body parts to be treated simultaneously in order to shorten the overall treatment time by the number of applicators 103 used to treat each of the individual body parts at the same time with their own applicator 103. For example, it would take one hour to treat four body separate body parts for 15 minutes each with a single applicator 103, while the same treatment could be reduced to 15 minutes if four applicators 103 were employed simultaneously.
  • treatment times can further reduced after the administration of supraparamagnetic iron oxide nanoparticles (SPIONs).
  • SPIONs supraparamagnetic iron oxide nanoparticles
  • the frequencies of the one or more carrier signals can be offset.
  • the offset can be (i) the Fibonacci sequence, (ii) the Pythagorean tuning ratio, (iii) the Phi ratio 1.618033, and (iv) frequency tones in the ratio of the circle of fifths.
  • standing wave ratios consistent with chords can be delivered in order to increase efficiency and tissue stimulatory effects for the purpose of physiological stimulation, magneto hydrodynamics and mechano- transduction.
  • strong resonant standing waves created by two or more applicators 103 can be utilized to enhance the nanooscillatory dissolutions of unwanted calcifications and intracellular debris.
  • This vibratory nanolithotripsy offers a non-invasive alternative to surgical stone removal in many parts of the body.
  • the ideal resonant frequency in the tissue can be determined as a function of shortest time of temperature rise per frequency set.
  • the ideal resonant frequency can be employed to find an optimum resonant frequency for the purpose of dissolving the stone.
  • the ionic water layer outside of microtubules is targeted in order to more efficiently target cellular functions.
  • the microtubules themselves are targeted in order more efficiently to target actin myosin and tubulin monomer stimulation through the efficient use of standing waves.
  • the ability to stimulate the micro-tubular surface structure in vivo allows for the non-invasive stimulation of microtubules in vivo for use in medical applications.
  • the energy transferred into the body by the stimulation with the or each ultra-high frequency resonant therapy applicator 103 is capable of promoting micro-tubular stability.
  • disposable, de-attachable, quick-disconnect bandage or stick-on electrolytic dielectric applicator pads for post-surgical application and therapy can be employed.
  • the or each applicator 103 is placed over a post-surgical site and adheres to the surface of the wound or area to be treated therapeutically for post-operative pain relief and wound rehabilitation.
  • the applicators 103 are of two formats, being (i) a dry bandage cotton surface applicator for incorporation into a bandage, and (ii) a hypoallergenic stick-on polymer gel applicator for direct site of interest application.
  • quick-disconnect radio frequency coax cables are attached to the patient bandage applicator or the hypoallergenic stick-on polymer gel applicator to the base unit radio frequency generator.
  • a quick-connect connector connects the temperature sensors from the applicators 103 to the base unit radio frequency generator.
  • Quick disconnects from the base unit to the applicators 103 allow the patient to only need to connect the cables to the applicators 103 during a therapy session and allow the patient to quickly disconnect the applicators 103 from the base unit.
  • the applicator bandage contains an EPROM memory storage in order to monitor therapy and provide a limited life use of the respective applicator 103.
  • the applicator bandage is programed to a limited number of uses in order to terminate the use of a bandage beyond its known safety ratings from a hardware prospective. In one embodiment the applicator bandage can be terminated after a certain period of time or by number of sessions for wound care and sterility. T ranscranial Mag netic Stimu fa tion
  • the apparatus can include a skull cap, which supports one of more applicators 103, for example, one, two, three or four applicators, for the application of AM modulated UHF energy for transcranial magnetic stimulation of brain tissue in humans and animals.
  • a skull cap optionally with Velcro (RTM) attachment, is made to receive a single patch antenna applicator 103 for AM modulated UHF application and stimulation of underlying brain tissue.
  • RTM Velcro
  • the single applicator 103 may be moved and positioned over the area of interest and secured in place to the skull cap.
  • the single applicator 103 is energized by an appropriate frequency composition over the area of interest for a user determined or machine determined time.
  • the skull cap is made to receive two patch antenna applicators 103 for AM modulated UHF application and stimulation of underlying brain tissue in stereo over opposing temporal lobes or over the frontal and occipital lobes.
  • the two applicators 103 are energized by an appropriate frequency composition over the area of interest for a user determined or machine determined time.
  • the double energized applicators 103 are set to apply a variety of phased intonations and harmonic vibratory compositions that allow the composition to energize and focus the UHF energy over opposing areas or targeted areas of the brain.
  • opposing applicators 103 may be set to offset frequencies in order to create a binaural signal that entrains brain signals.
  • the applicators 103 may be offset by from 0 Hz to about 35 Hz or other user-specified harmonic ratios.
  • the three applicators 103 are energized by an appropriate frequency composition over the area of interest for a user determined or machine determined time.
  • the triple-energized applicators 103 are set to apply a variety of phased intonations and harmonic vibratory compositions that allow the composition to energize and focus the UHF energy over opposing areas or targeted areas of the brain.
  • Triplet harmonic tunings allow chords of vibratory energy to be tuned and focused.
  • the four applicators 103 are energized by an appropriate frequency composition over the area of interest for a user determined or machine determined time.
  • the quadruple energized applicators 103 are set to play a variety of phased intonations and harmonic vibratory compositions that allow the composition to energize and focus the UHF energy over single areas or group targeted areas of the brain.
  • Quadruple harmonic tunings allow chords of vibratory energy to be tuned and focused.
  • binaural stimulation in stereo and in phased array focusing is possible with the double, triple and quadruple applicator configurations.
  • chords may be created with Pythagorean tuning of base and subsequent frequencies in order to increase the resonance factor of stimulated tissues.
  • the apparatus can be employed to pre-heat major muscle groups of an athlete prior to an event and cause vasodilation with accompanying oxygen delivery to tissues. Studies show that heating muscles to from 37 °C to 41 °C produces HSPs, which help form stronger tendons and ligaments and decrease sport/training related injuries, so trainers have investigated pre-warming during training for pre-conditioning.
  • pre-conditioning composition set is as follows:

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Abstract

L'invention concerne un appareil ou un procédé de création d'ondes stationnaires résonantes dans un tissu biologique d'un sujet dans le traitement de la mastite, en particulier de la mastite bovine, comprenant l'application simultanée de signaux de porteuse modulés en amplitude et en fréquence à un sujet afin de créer des ondes stationnaires résonantes dans le tissu.
PCT/EP2018/085839 2017-12-19 2018-12-19 Appareil et procédé de création d'ondes stationnaires résonantes dans un tissu biologique WO2019121911A1 (fr)

Applications Claiming Priority (2)

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