WO2019113880A1 - Agent protecteur pour des ressources osseuses anciennes - Google Patents

Agent protecteur pour des ressources osseuses anciennes Download PDF

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Publication number
WO2019113880A1
WO2019113880A1 PCT/CN2017/116123 CN2017116123W WO2019113880A1 WO 2019113880 A1 WO2019113880 A1 WO 2019113880A1 CN 2017116123 W CN2017116123 W CN 2017116123W WO 2019113880 A1 WO2019113880 A1 WO 2019113880A1
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WIPO (PCT)
Prior art keywords
parts
ancient
weight
water
ancient bone
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PCT/CN2017/116123
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English (en)
Chinese (zh)
Inventor
吕绍武
张全超
朱泓
葛丹阳
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吉林大学
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Application filed by 吉林大学 filed Critical 吉林大学
Priority to PCT/CN2017/116123 priority Critical patent/WO2019113880A1/fr
Priority to AU2018100753A priority patent/AU2018100753A4/en
Priority to ZA2018/04005A priority patent/ZA201804005B/en
Publication of WO2019113880A1 publication Critical patent/WO2019113880A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D101/00—Coating compositions based on cellulose, modified cellulose, or cellulose derivatives
    • C09D101/02—Cellulose; Modified cellulose
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D103/00—Coating compositions based on starch, amylose or amylopectin or on their derivatives or degradation products
    • C09D103/02—Starch; Degradation products thereof, e.g. dextrin
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D189/00—Coating compositions based on proteins; Coating compositions based on derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
    • C09J11/00—Features of adhesives not provided for in group C09J9/00, e.g. additives

Definitions

  • the invention relates to the field of ancient bone resource protection, in particular to an ancient bone resource protection agent.
  • China's history has a long history and has a long history. It has a history of more than 5,000 years.
  • Ancient ruins are one of the most important Chinese cultural resources reserved for future generations. They are also precious national treasures.
  • the cultural values they imply and the call for national consciousness and national consciousness are much higher than the value of material wealth.
  • the remains unearthed from the remains of the site are important cultural heritages reflecting the origin and development of the Chinese nation, a valuable material evidence of the history of Chinese civilization and culture, an important carrier of the history of civilization development in China for thousands of years, and a history of Chinese history.
  • the “treasures” in the cultural heritage, these ancient bone resources are unique, irreplaceable, non-renewable, and it is urgent to properly preserve it.
  • resin-based protective agents including: natural resin and nitro varnish, acrylic colloid dispersion system, acrylic emulsion product, polyvinyl acetate, polyvinyl alcohol Butyraldehyde, acrylic resin, trimethyl resin (methyl methacrylate-butyl methacrylate-methacrylic acid copolymer), in addition to polyethylene glycol (PEG) protective agent, but with people Their use, their shortcomings are gradually exposed, and their common shortcomings are poor reversibility and toxicity.
  • resin-based protective agents including: natural resin and nitro varnish, acrylic colloid dispersion system, acrylic emulsion product, polyvinyl acetate, polyvinyl alcohol Butyraldehyde, acrylic resin, trimethyl resin (methyl methacrylate-butyl methacrylate-methacrylic acid copolymer), in addition to polyethylene glycol (PEG) protective agent, but with people Their use, their shortcomings are gradually exposed, and their common shortcomings are poor reversibility and toxicity.
  • PEG polyethylene glycol
  • Natural resin and nitro varnish have poor reinforcement, and they have a certain color, which is not conducive to the protection and ornamental effect of the remains. It has not been put into use at present; the dispersion of acrylic colloid has not been produced in China, and the utilization rate is very small; The emulsion product has poor reversibility and is not used frequently. Polyvinyl acetate is not only poorly reversible, but its anti-aging effect can not meet people's needs.
  • polyvinyl butyral The reversibility of polyvinyl butyral is better than the above two materials, but the aging resistance effect Poor, irreversible after aging, damage to the bones; acrylic resin will affect the values of ⁇ 15 N and ⁇ 13 C, which is not conducive to the identification of the bones, and is often protected by acrylic resin after sampling; A protective agent used, which does not affect the identification of the sample, but has poor reversibility, and is dissolved by toxic organic reagents such as toluene and chloroform, posing a certain threat to the health of operators and visitors, and also has a certain environmental impact. Hazard. In addition to resin-based materials, polyethylene glycol (PEG) is also used for the protection of ancient bone resources, but it is easy to adhere to dust in high-humidity environments and is not currently in use.
  • PEG polyethylene glycol
  • Poor reversibility means that after the resin-based protective agent is applied to ancient bones by spraying or dripping, the curing action of the resin-based protective agent can reinforce the ancient bones, but at the same time, between the ancient bones and between the ancient bones and the soil. The blockage makes it difficult to remove the cured resin-based protective agent and does not return the ancient bones to the original bone texture state.
  • the disadvantage of poor reversibility of the resin-based protective agent limits the re-acquisition and subsequent research of ancient bone samples using the resin as a protective measure.
  • Too much toxicity means that the resin-based protective agent often uses toluene and chloroform as solvents.
  • mild exposure to chloroform can cause conjunctival congestion and skin edema, and long-term exposure can lead to many diseases such as embryo toxicity, teratogenicity, liver necrosis or liver cancer, so the resin protective agent is operated.
  • the body is very toxic.
  • the resin protection agent is also harmful to the visitors.
  • Resin solvents such as chloroform and toluene can enter the visitor by inhalation or absorption through the skin, irritating the mucous membrane and anesthetizing the central nervous system, causing dizziness, headache, vomiting, weakness of the limbs, and confusion.
  • the present invention relates to the following:
  • An ancient bone resource protecting agent which comprises the following components: 10-25 parts of a binder, 10-25 parts of propylene glycol, 0.01-0.3 parts of an antioxidant enzyme mimetic according to 100 parts by weight of a protective agent, Plant extract antioxidant 0.01-0.05 parts, preservative 0.001-0.003 parts, lysozyme 0.1-0.3 parts, the balance is water;
  • the binder includes any one of a water-soluble protein and a water-soluble starch.
  • the ancient bone resource protecting agent according to Item 1 which comprises the following components: 12-22 parts by weight of the protective agent, 12-23 parts of propylene glycol, and 0.02 of antioxidant enzyme mimetic. - 0.25 parts, plant extract antioxidant 0.02-0.05 parts, preservative 0.0015-0.0025 parts, lysozyme 0.15-0.28 parts, the balance is water.
  • the ancient bone resource protecting agent according to Item 1 or 2 characterized in that it comprises the following components: According to 100 parts by weight of the protective agent, 13-20 parts of binder, 13-31 parts of propylene glycol, 0.04-0.22 parts of antioxidant enzyme mimetic, 0.03-0.045 parts of plant extract antioxidant, 0.0017-0.0023 parts of preservative, lysing The enzyme is 0.18-0.25 parts, and the balance is water.
  • the ancient bone resource protecting agent according to any one of items 1 to 4, wherein the binder is a water-soluble protein, and the water-soluble protein is 10-part by weight of the protective agent. 25 parts, preferably 12-22 parts of water-soluble protein, further preferably 13-20 parts of water-soluble protein.
  • the ancient bone resource protecting agent according to any one of items 1 to 5, wherein the binder is a water-soluble starch, and the water-soluble starch is 10-part by weight of the protective agent. 25 parts, preferably water soluble starch is 16-18 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 6, wherein the preservative is sorbic acid and a salt thereof, sodium hydroxymethylglycine, polyaminopropyl biguanide, double ( Any one or more of hydroxymethyl)imidazolidinyl urea, imidazolidinyl urea.
  • the ancient bone resource protecting agent according to any one of items 1 to 7, wherein the preservative is sorbic acid and a salt thereof, and the sorbic acid and the sorbic acid are 100 parts by weight of the protective agent.
  • the salt is from 0.002 to 0.006 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 8, wherein the preservative is sodium hydroxymethylglycine, and the sodium hydroxymethylglycine is 100 parts by weight of the protective agent. It is 0.002-0.005 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 9, wherein the preservative is polyaminopropyl biguanide, and the polyaminopropyl biguanide is 100 parts by weight of the protective agent. It is 0.002 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 10, wherein the preservative is bis(hydroxymethyl)imidazolidinyl urea, and the weight of the protective agent is 100 parts.
  • the bis(hydroxymethyl)imidazolidine urea is from 0.002 to 0.005 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 11, wherein the preservative is imidazolidinyl urea, and the imidazolidinyl urea is 0.002 by weight of the protective agent. -0.006 parts.
  • the ancient bone resource protecting agent according to any one of the items 1 to 12, wherein the plant extract antioxidant is any one or more of a tea polyphenol and a rosemary extract.
  • the ancient bone resource protecting agent according to any one of items 1 to 13, wherein the plant extract antioxidant is tea polyphenol, and the tea polyphenol is 100 parts by weight of the protective agent. 0.01-0.05 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 14, wherein the plant extract antioxidant is a rosemary extract, and the rose is 100 parts by weight of the protective agent.
  • the fragrant extract is 0.01-0.05 parts.
  • the ancient bone resource protecting agent according to any one of items 1 to 15, wherein the antioxidant enzyme mimetic is a selenium-containing cyclodextrin having glutathione peroxidase activity, Any one or more of selenopeptides and selenoproteins.
  • the ancient skeletal resource protecting agent according to any one of items 1 to 16, wherein the antioxidant enzyme mimetic is a selenium-containing cyclodextrin, a selenopeptide, a selenoprotein, and a weight of a protective agent.
  • the antioxidant enzyme mimetic is 0.01-0.3 parts in 100 parts.
  • the ancient bone resource protecting agent provided by the invention comprises the following components: 10-25 parts by weight of the protective agent, 10-25 parts of propylene glycol, 0.01-0.3 parts of antioxidant enzyme mimetic, and plant extracting antioxidant 0.01 according to the weight of the protective agent. -0.05 parts, preservative 0.001-0.003 parts, lysozyme 0.1-0.3 parts, the balance is water;
  • the ancient bone resource protecting agent provided by the invention comprises any one of a water-soluble protein and a water-soluble starch.
  • One of water-soluble protein and water-soluble starch and propylene glycol can be used as a raw material of the substrate, and the base component of the ancient bone resource protecting agent can be formed by mixing.
  • the two raw materials of the substrate are not only low in toxicity but also have good water solubility.
  • Sex Sex
  • a film is formed, which realizes the reversibility and protection of ancient bone resource protection agents; by adding non-toxic or low-toxic antioxidant components - Antioxidant enzyme mimics, plant extract antioxidants, and the ancient skeletal resource protectant's antioxidant capacity, reducing the oxidative damage of ancient oxygen resources to the ancient bone resources during excavation, transportation and display; by adding specific content
  • the antiseptic component preservative and lysozyme
  • the obtained ancient bone resource protection agent has a dynamic viscosity value ⁇ of 5-300 mPa ⁇ s (measured by a NDJ-1 rotary viscometer), and has good reversibility, protection, anti-oxidation and antibacterial ability after spraying ancient bones. .
  • the ancient bone resource protecting agent provided by the invention comprises the following components: 12-22 parts by weight of the protective agent, 12-23 parts of propylene glycol, 0.02-0.25 parts of antioxidant enzyme mimetic, plant extracting 0.02-0.05 parts of antioxidant, 0.0015-0.0025 parts of preservative, 0.15-0.28 parts of lysozyme, The amount is water.
  • one of the water-soluble protein and water-soluble starch and propylene glycol can be used as a raw material of the substrate, and the base component of the ancient bone resource protecting agent can be formed by mixing, and the raw materials of the two substrates are not only very toxic. It is low and has good water solubility; after a certain proportion of the ancient bone resource protection agent is sprayed onto the ancient bone resources, it will form a protective film on the surface to protect the ancient bone resources. Because the two substrates have good water solubility, the protective film formed on the surface of ancient bone resources can be easily washed away with water, and the ancient bone resources return to the original bone texture state, which realizes the protection of ancient bone resources. The reversibility of the agent.
  • the antioxidant enzyme mimetic is selected from any one or more of selenium-containing cyclodextrin and selenopeptide-containing. Selenium is an essential trace element in the human body and has long been regarded as an important antioxidant.
  • Selenium is also a component of the important antioxidant enzyme, glutathione peroxidase, which guarantees glutathione peroxidase. It removes active oxygen and exerts anti-oxidation effect, which can effectively prevent oxidation reaction of biomolecules.
  • Selenium-containing cyclodextrin is a polysaccharide containing selenium.
  • the oral administration of LD50 is 3.6g/kg (low toxicity) in mice.
  • the selenopeptide is a peptide molecule containing selenium.
  • the oral LD50 of mice is 2.8-4.1g/kg (low toxicity). ).
  • the selenium-containing cyclodextrin, selenopeptide and other molecules have glutathione peroxidase activity (called antioxidant enzyme mimic), which not only has antioxidant activity, but also has stronger stability than natural enzymes, and is more suitable.
  • antioxidant enzyme mimic glutathione peroxidase activity
  • the plant extracting antioxidant is selected from any one or more of tea polyphenols and rosemary extracts. Tea polyphenols are the main active substances in tea. They are non-toxic natural antioxidants with strong anti-oxidation properties. As early as 1995, China Light Industry Association applied them as antioxidants in the food industry. Approved food additives are widely used in food preservation. Rosemary extract is a natural antioxidant that protects biomolecules against oxidation.
  • the antioxidants in this extract are mainly rosmarin, rosmarinic acid, ursolic acid, carnosol, etc.
  • Rosemary extract has been widely used in air cleaners, perfumes, soaps, lotions, and in beverages, skin oils, and hair tonics.
  • the above-mentioned antioxidant component is applied to the ancient bone resource protection agent not only to prevent oxidative damage of ancient bone resources, but also to protect the safety of operators.
  • the selenopeptide-containing one of Arg-Gly-Sec-Arg-Asn and Sec-Arg-Gly-Asp is selected One or more, in the selenopeptide-containing Sec is selenocysteine.
  • the selenopeptide-containing peptide is Arg-Gly-Sec-Arg-Asn
  • the selenopeptide-containing peptide is 0.025-0.045 parts by weight of 100 parts by weight of the protective agent.
  • the selenopeptide-containing peptide is 0.03-0.04 parts. It has been verified that the ancient skeletal resource protectant formulation contains the parts by weight of the selenopeptide, and the anti-oxidation effect is good.
  • the selenopeptide-containing peptide is Sec-Arg-Gly-Asp, and the selenopeptide-containing peptide is 0.03-0.05 parts by weight of 100 parts by weight of the protective agent. Further, the selenopeptide-containing peptide is from 0.035 to 0.045 parts. It has been verified that the ancient skeletal resource protectant formulation contains the parts by weight of the selenopeptide, and the anti-oxidation effect is good.
  • Lysozyme is a non-toxic, non-side-effect protein with natural antibacterial activity, decomposing Bacillus subtilis and radiation-resistant micrococcal bacteria in Gram-positive bacteria, against Escherichia coli, common proteobacteria and parahemolytic arc Gram-negative bacteria such as bacteria also have a certain dissolution effect, and have been widely used in the preservation of aquatic products, meat products, cakes, milk powders and beverages.
  • the above antiseptic component does not need to be heated during the sterilization and antiseptic process, so it can be applied to the protection of ancient bone resources without damaging the ancient bone resources.
  • the binder is selected from the nutrients of the daily diet of human beings, and the human is non-toxic.
  • the ancient bone resource protecting agent provided by the embodiment of the invention comprises the following components: 13-20 parts by weight of the protective agent, 13-21 parts of propylene glycol, and 0.04-0.22 of the antioxidant enzyme mimetic. Ingredients, plant extract antioxidant 0.03-0.045 parts, preservative 0.0017-0.0023 parts, lysozyme 0.18-0.25 parts, the balance is water.
  • the water-soluble protein selects bovine serum albumin, soybean water-soluble protein, has a good adhesion effect, and is easy to wash off.
  • the water-soluble protein is any one or more of bovine serum albumin and soybean water-soluble protein, and the water-soluble protein is 14-19 parts by weight of 100 parts by weight of the protective agent.
  • the binder is a water-soluble starch
  • the water-soluble starch is 15-18 parts by weight of 100 parts by weight of the protective agent. It has been verified that the ancient bone resource protectant formulation contains the parts by weight of water-soluble starch, and the reversibility effect is good.
  • the preservative is selected from any one or more of sorbic acid and its salts, sodium hydroxymethylglycine, polyaminopropyl biguanide, and imidazolidinyl urea.
  • the maximum allowable concentration of sorbic acid and its salt preservatives is 0.6%, and the maximum allowable concentration of sodium hydroxymethylglycinate is 0.5%.
  • the maximum allowable concentration of propyl biguanide is 0.3%, and the maximum allowable concentration of imidazolidinyl urea is 0.6%.
  • the preservatives selected in the present invention are all lower than the above technical specifications, indicating that the safety is high.
  • the preservative is sodium sorbate
  • the sodium sorbate is from 0.0013 to 0.0027 parts by weight of the protective agent.
  • the sodium sorbate is from 0.0015 to 0.002 parts. It has been verified that the ancient bone resource protectant formulation contains the parts by weight of sodium sorbate, and has good antiseptic and antibacterial effects.
  • the preservative is sodium hydroxymethylglycine
  • the sodium hydroxymethylglycine is 0.0012-0.0025 parts by weight of 100 parts by weight of the protective agent; further, the sodium hydroxymethylglycine is 0.002-0.0023 parts.
  • the ancient skeletal resource protectant formulation contains the parts by weight of sodium hydroxymethylglycine, and has good antiseptic and antibacterial effects.
  • the preservative is polyaminopropyl biguanide
  • the polyaminopropyl biguanide is 0.001-0.002 parts by weight of 100 parts by weight of the protective agent; further, the polyaminopropyl biguanide is 0.0013-0.0017. It has been verified that the ancient bone resource protectant formulation contains the parts by weight of polyaminopropyl biguanide, and has good antiseptic and antibacterial effect.
  • the preservative is imidazolidinyl urea
  • the imidazolidinyl urea is 0.0016-0.0028 parts by weight of the protective agent
  • the imidazolidinyl urea is 0.0018-0.0026. It has been verified that the ancient bone resource protectant formulation contains the parts by weight of imidazolidinyl urea, and has good antiseptic and antibacterial effects.
  • the ancient bone resource protecting agent provided by the embodiment of the invention uses a binder and propylene glycol as a substrate, and selects a specific kind of antioxidant (antioxidant enzyme mimetic, plant extract antioxidant), lysozyme, and is mixed with water. Through the combination of different material components and their contents, each component synergizes with each other, and it has very good reversibility, protection, anti-oxidation and antibacterial ability after spraying ancient bones. Moreover, the components of the ancient bone resource protecting agent provided by the invention are non-toxic or low-toxic substances, and the added amount thereof refers to the national food or cosmetic standard, and the obtained bone resource protecting agent has no damage to the human body, and the operator is guaranteed. And The safety of the viewer.
  • An ancient bone resource protecting agent comprising the following components: 10 parts by weight of protective agent, 10 parts of bovine serum albumin, 10 parts of propylene glycol, 0.01 part of selenium cyclodextrin, 0.01 part of tea polyphenol, 0.001 part of sodium sorbate , 0.1 part of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 15 parts of soybean water-soluble protein, 15 parts of propylene glycol, 0.03 parts of selenopeptide (Arg-Gly-Sec-Arg-Asn), and 10 parts by weight of protective agent 0.04 parts of fragrant extract, 0.002 parts of sodium hydroxymethylglycine, 0.2 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protection agent comprising the following components: 25 parts by weight of protective agent, 25 parts of bovine serum albumin, 25 parts of propylene glycol, 0.3 parts of selenium cyclodextrin, 0.05 parts of tea polyphenol, 0.003 parts of sodium sorbate , lysozyme 0.3 parts, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protection agent comprising the following components: 10 parts of bovine serum albumin, 25 parts of propylene glycol, 0.01 parts of selenium cyclodextrin, 0.03 parts of tea polyphenol, polyaminopropyl biguanide according to 100 parts by weight of protective agent 0.0045 parts, 0.2 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 17 parts of soybean water-soluble protein, 18 parts of propylene glycol, and 0.035 parts of selenopeptide (Arg-Gly-Sec-Arg-Asn) according to 100 parts by weight of protective agent, 0.036 parts of fragrant extract, 0.0021 parts of sodium hydroxymethylglycine, 0.21 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 10 parts of water-soluble starch, 10 parts of propylene glycol, 0.01 parts of Arg-Gly-Sec-Arg-Asn, rosemary according to 100 parts by weight of protective agent 0.01 parts of extract, 0.001 parts of imidazolidinyl urea, 0.1 part of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 25 parts of water-soluble starch, 25 parts of propylene glycol, 0.3 parts of selenium-containing cyclodextrin, 0.05 parts of tea polyphenol, 0.003 parts of sodium sorbate, according to 100 parts by weight of the protective agent. 0.3 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 16 parts of water-soluble starch, 18 parts of propylene glycol, 0.17 parts of selenium cyclodextrin, 0.039 parts of tea polyphenol, and 0.0018 parts of sodium sorbate according to 100 parts by weight of protective agent. 0.23 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 11 parts of water-soluble starch, 20 parts of propylene glycol, 0.03 parts of sele-containing peptide (Sec-Arg-Gly-Asp), rosemary extract according to 100 parts by weight of protective agent 0.033 parts, 0.0036 parts of sodium hydroxymethylglycine, 0.2 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • An ancient bone resource protecting agent comprising the following components: 20 parts of water-soluble protein, 22 parts of propylene glycol, 0.016 parts of sele-containing peptide (Sec-Arg-Gly-Asp), rosemary extract according to 100 parts by weight of protective agent 0.034 parts, 0.0015 parts of polyaminopropyl biguanide, 0.26 parts of lysozyme, the balance is water; the content of each component is mixed to obtain the spraying agent of ancient bone resource protection agent.
  • the ancient bone resource protecting agents prepared in Examples 1-10 were tested for reversibility, protection, oxidation resistance and antibacterial properties, respectively.
  • the ancient bone resource protecting agents prepared in Examples 1-3 and 10 were determined by fluorescence labeling for reversibility.
  • Example 4-6 The ancient bone resource protection agent prepared in Example 4-6 was tested for reversibility by means of iodine water.
  • the spraying of the ancient bone resource protection agent is carried out: the thickness of the protective film is 45-55 ⁇ m, the value of ⁇ is 60% ⁇ ⁇ ⁇ 73%; the spraying of the ancient bone resource protection agent is carried out twice: protection The film thickness is 75-95 ⁇ m, the ⁇ value range is 95% ⁇ 143%; 3 times of ancient bone resource protection agent spraying: the protective film thickness is 115-135 ⁇ m, the ⁇ value range is 139% ⁇ 201%; It can be seen from the above data that the ancient bone resource protection agent provided by the embodiment of the present invention can effectively form a protective film on the bone surface and enhance the bone hardness by spraying one or more times on the ancient bone.
  • the bone hardness reduction rate at 6 months and 12 months after spraying was measured.
  • the archaeological excavation process is a sudden change of the environment of ancient bone resources.
  • the above ground environment including temperature, humidity, light, bacteria, etc.
  • the protection of ancient bone resources can be divided into two types: “in situ protection” and “extraction protection”.
  • In-situ protection refers to the site protection that does not change the excavation location of the ancient bones; “extraction protection” refers to the protection of ancient bones during the process of peeling ancient bones from the buried soil. The first movement after the cultural relics were unearthed, the quality of protection will directly affect the future display and research work.
  • the in-situ protection work is relatively simple, and the ancient bone resource protection agent provided by the invention is sprayed on the ancient bone exposed on the surface of the soil to a thickness of 40-140 ⁇ m, and the ancient bone resource protection agent is infiltrated into the soil, and the area is reached.
  • the surface of the ancient bones exposed to the surface of the earth is more than 1.5 times and the depth is more than 0.3m, thus achieving the role of protecting ancient bones below the surface of the earth.
  • the extraction and protection work must first evaluate the degree of fragmentation (completeness, rupture, porosity, decay, chalking, etc.) and hardness of the extracted ancient bone resources.
  • the ancient bone resource protection agent provided by the invention is injected into the ancient bone by multiple in-situ drip infiltration method, so that the purpose of ancient bone extraction can be satisfied.
  • Ancient bones were bonded by the adhesion of ancient bone resource protectants, and the hardness of ancient bones was also increased after the ancient bone resource protectants dried, and then used for subsequent display and scientific research.
  • the ancient bone preservation conditions are different, and the protection effect of ancient bone resource protection agents is also different. It can be used to track the evaluation of the ancient bones and the work of the spray protection agent in 3 months or 6 months.
  • the diameter of the antibacterial ring of the ancient bone resource protection agent is more than 12mm, which proves that the antibacterial effect is obvious.
  • the ancient bone resource protecting agent provided by the present invention, it can be presumed that it can be used for inhibiting mold.
  • the ancient bone resource protecting agent provided by the embodiment of the invention uses one or more of water-soluble protein and water-soluble starch and propylene glycol as a substrate for an ancient bone resource protecting agent, and the two substrates are not only low in toxicity but also have Very good water solubility. After a certain proportion of the ancient bone resource protection agent is sprayed onto the ancient bone resources, a protective film is formed on the surface to protect the ancient bone resources.
  • the protective film formed on the surface of the ancient bone resources can be conveniently washed away with water, and the ancient bone resources are returned to the original state, thereby realizing the reversibility of the ancient bone resource protecting agent;
  • Non-toxic or low-toxic natural antioxidant enzymes glutathione peroxidase
  • natural antioxidants plant extract antioxidants

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Abstract

L'invention concerne un agent protecteur pour des ressources osseuses anciennes, qui est préparé par mélange d'un liant, de propylène glycol, d'un mimétique d'enzyme antioxydant, d'un antioxydant d'extrait végétal, d'un agent antibactérien, d'un conservateur, d'un lysozyme et d'eau. Le liant contient soit une protéine soluble dans l'eau soit un amidon soluble dans l'eau. L'effet synergique des composants de l'agent protecteur est amélioré par la coordination de différents ingrédients de substance et de leurs contenus. L'agent protecteur présente une excellente réversibilité, une excellente protection, une excellente résistance à l'oxydation et une excellente capacité antibactérienne après avoir été pulvérisé sur des os anciens. L'agent protecteur ne nuit pas au corps humain et garantit la sécurité des opérateurs et des visiteurs.
PCT/CN2017/116123 2017-12-14 2017-12-14 Agent protecteur pour des ressources osseuses anciennes WO2019113880A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/CN2017/116123 WO2019113880A1 (fr) 2017-12-14 2017-12-14 Agent protecteur pour des ressources osseuses anciennes
AU2018100753A AU2018100753A4 (en) 2017-12-14 2018-06-06 Ancient bone resource protective agent
ZA2018/04005A ZA201804005B (en) 2017-12-14 2018-06-15 Ancient bone resource protective agent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/116123 WO2019113880A1 (fr) 2017-12-14 2017-12-14 Agent protecteur pour des ressources osseuses anciennes

Publications (1)

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WO2019113880A1 true WO2019113880A1 (fr) 2019-06-20

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PCT/CN2017/116123 WO2019113880A1 (fr) 2017-12-14 2017-12-14 Agent protecteur pour des ressources osseuses anciennes

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AU (1) AU2018100753A4 (fr)
WO (1) WO2019113880A1 (fr)
ZA (1) ZA201804005B (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109517524A (zh) * 2018-12-07 2019-03-26 吉林大学 一种古代骨骼资源保护剂

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0852992A2 (fr) * 1997-01-08 1998-07-15 Dow Corning Corporation Conservation des matériaux organiques et inorganiques
US6835411B1 (en) * 1997-01-08 2004-12-28 Dow Corning Corporation Conservation of organic and inorganic materials
CN104152046A (zh) * 2014-08-08 2014-11-19 吉林大学 一种古代骨骼资源保护剂

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0852992A2 (fr) * 1997-01-08 1998-07-15 Dow Corning Corporation Conservation des matériaux organiques et inorganiques
US6835411B1 (en) * 1997-01-08 2004-12-28 Dow Corning Corporation Conservation of organic and inorganic materials
CN104152046A (zh) * 2014-08-08 2014-11-19 吉林大学 一种古代骨骼资源保护剂

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AU2018100753A4 (en) 2018-07-12
ZA201804005B (en) 2019-04-24

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