WO2019095286A1 - 一种生物实验用冰袋及其使用方法 - Google Patents

一种生物实验用冰袋及其使用方法 Download PDF

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WO2019095286A1
WO2019095286A1 PCT/CN2017/111674 CN2017111674W WO2019095286A1 WO 2019095286 A1 WO2019095286 A1 WO 2019095286A1 CN 2017111674 W CN2017111674 W CN 2017111674W WO 2019095286 A1 WO2019095286 A1 WO 2019095286A1
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ice
ice pack
main
ice bag
pack
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PCT/CN2017/111674
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English (en)
French (fr)
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王建坤
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盐城天顺机械科技有限公司
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Priority to PCT/CN2017/111674 priority Critical patent/WO2019095286A1/zh
Publication of WO2019095286A1 publication Critical patent/WO2019095286A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
    • F25D3/02—Devices using other cold materials; Devices using cold-storage bodies using ice, e.g. ice-boxes

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  • the invention belongs to the technical field of molecular biology, and particularly relates to an ice bag for biological experiments and a using method thereof.
  • the enzyme in the upper part of the centrifuge tube may be partially deactivated, affecting the experimental results, and may even cause the experiment to start again.
  • the object of the present invention is to provide a biological experiment ice pack and a use method thereof, which can quickly make the working fluid in the centrifuge tube, especially the 15 ml or 50 ml centrifuge tube, quickly reach a low temperature state, and at the same time keep the low temperature for a long time, thereby ensuring The activity of the enzyme in the working fluid ensures the success rate of the experiment and avoids possible repeated experiments.
  • An ice pack for biological experiment comprising a main ice pack for placing a centrifuge tube rack, and a side ice pack capable of flipping 360 degrees around the main ice pack is hinged at an edge of the main ice pack, and the main ice pack and the sub-ice pack are both
  • the utility model is composed of a rectangular box-shaped plastic box body containing contents, wherein the main ice bag is hinged with a plurality of telescopic brackets, and the sub-ice bag is provided with a plurality of grooves, and the groove is along the main body and the main body.
  • One end of the ice bag hinged is arranged away from one end of the main ice bag, and an elastic ring is disposed in the groove, and an end of one end of the bracket is movably inserted into the groove and closely attached to the elastic ring .
  • the centrifuge tube When the centrifuge tube is relatively large, placed on the main ice pack, it can receive the low temperature transfer of the sub-ice pack except the main ice pack, and the accommodation for accommodating the centrifuge tube formed between the main ice pack and the sub-ice pack
  • the space is slower in heat dissipation than only one of the main ice packs, so that the working fluid in the centrifuge tube placed on the centrifugal tube rack on the main ice pack can be quickly brought to a low temperature state, and can be maintained for a long time.
  • the low temperature state can maintain the activity of the enzyme in the working fluid, ensure the success rate of the experiment, and avoid possible repeated experiments.
  • the auxiliary ice pack is provided at an edge of the four sides of the main ice pack.
  • the centrifuge tube placed on the centrifuge tube can be subjected to cold air transmission in five directions, thereby maintaining it at a low temperature.
  • a side of the main ice bag hinged with the sub-ice bag is provided with a pivot, two ends of the sub-ice bag are provided with a bump, and the protrusion is provided with a through hole, and the two of the pivot The ends are respectively inserted into the two through holes.
  • the interior of the pivot is hollow, and the cavity is filled with the contents.
  • the pivot also functions as an ice pack to further maintain a low temperature.
  • one, two or more of the brackets are disposed between the main ice pack and one of the sub-ice packs.
  • the content is a high molecular polymer, a water absorbing resin or water.
  • a method of using an ice pack for a right biological experiment comprising the steps of:
  • step S2 adjusting a positional relationship between the main ice pack and the sub-ice pack according to the size of the centrifuge tube rack in step S1, thereby adjusting an opening size of an accommodation space formed between the main ice pack and the sub-ice pack
  • the bracket is contracted, the bracket is attached to one side of the main ice bag, and the sub-ice bag is turned over, such that one side of the auxiliary ice bag and one of the main ice bag are The side faces are attached, and one side of the main ice pack that is attached to the sub-ice bag and one side of the main ice pack provided with the bracket are parallel to each other, and are placed in a refrigerator for refrigeration.
  • This collection of ice packs can reduce the storage space occupied by the ice packs.
  • the invention has the beneficial effects that the working fluid in the centrifuge tube, especially the 15 ml or 50 ml centrifuge tube can be quickly brought to a low temperature state, and the temperature is kept low for a long time, and can be kept in the working fluid, compared with only one main ice pack.
  • the activity of the enzyme ensures the success rate of the experiment and avoids repeated experiments.
  • Figure 1 is a schematic view of the structure of the present invention
  • Figure 2 is a schematic view of the mechanism of the sub-ice bag
  • Figure 3 is a plan view of a state of use of the present invention.
  • the figure is marked as: 1, the main ice bag; 2, the auxiliary ice bag; 3, the bracket; 4, the groove; 41, the elastic ring; 5, the pivot; 6, the bump; 61, the through hole; ; 8, centrifuge tube.
  • a biological experiment ice bag includes a main ice bag 1 for placing a centrifuge tube rack 7, and a sub-ice bag 2 capable of 360-degree turning around the main ice bag 1 is hinged at an edge of the main ice bag 1.
  • the ice packs 2 are provided at the edges of the four sides of the main ice pack 1, and the main ice packs 1 and the sub-ice packs 2 are each formed of a plastic box having a rectangular parallelepiped shape containing contents, and the contents are high molecular polymers and water absorbing resins. Or water.
  • the main ice pack 1 is hinged with a plurality of telescopic brackets 3, and the auxiliary ice pack 2 is provided with a plurality of grooves 4 arranged along a direction away from the end hinged with the main ice pack 1 away from the end of the main ice pack 1, the groove 4
  • An elastic ring 41 is disposed therein, and an end of one end of the bracket 3 is movably inserted into the recess 4 and closely adheres to the elastic ring 41.
  • the accommodating space allows the centrifugal tube 8 placed on the centrifuge tube rack 7 to receive the cold air transmission in five directions, so that it is maintained at a low temperature state, and the heat dissipation is slow, enabling placement.
  • the working fluid in the centrifuge tube 8 on the centrifugal tube rack 7 on the main ice pack 1 quickly reaches a low temperature state, and can maintain a low temperature state for a long time, can maintain the activity of the enzyme in the working fluid, and ensure the success rate of the experiment, avoiding the possibility Repeated experimentation.
  • a side portion of the main ice pack 1 hinged to the auxiliary ice pack 2 is provided with a pivot 5, and two ends of the auxiliary ice pack 2 are provided with a protrusion 6 provided with a through hole 61, and two ends of the pivot shaft 5 are respectively Inserted into the two through holes 61.
  • This is a hinged manner in which the sub-ice bag 2 is rotated about the pivot 5 to adjust the positional relationship with the main ice pack 1, thereby adjusting the size of the opening in which the accommodation space of the centrifuge tube rack 7 is placed.
  • the interior of the pivot 5 is hollow, and the cavity of the pivot 5 contains contents.
  • the pivot 5 also functions as an ice pack to further maintain a low temperature.
  • brackets 3 are provided between the main ice pack 1 and one of the sub-ice packs 2.
  • a method of using an ice pack for biological experiments comprising the steps of:
  • the bracket 3 is contracted, the bracket 3 is attached to one side of the main ice pack 1, and the sub-ice pack 2 is turned over, so that one side of the sub-ice pack 2 is attached to one side of the main ice pack 1, and the pair One side of the main ice pack 1 to which the ice pack 2 is attached and one side of the main ice pack 1 provided with the rack 3 are parallel to each other, and are placed in a refrigerator for refrigeration.

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种生物实验用冰袋及其使用方法，涉及分子生物学技术领域，包括用于放置离心管架（7）的主冰袋（1），主冰袋（1）的边缘处铰接有能够绕所述主冰袋（1）360度翻转的副冰袋（2），主冰袋（1）和副冰袋（2）均由长方体形的内部装有内容物的塑料盒体构成，主冰袋（1）上铰接有能够伸缩的支架（3），副冰袋（2）上设有若干凹槽（4），所述凹槽（4）沿从与所述主冰袋（1）铰接的一端向远离所述主冰袋（1）的一端的方向排列，凹槽（4）内设有弹性圈（41），所述支架（3）一端的端部活动插入所述凹槽（4）内并与所述弹性圈（41）紧密相贴；能够快速的使离心管尤其是15ml或50ml的离心管内的工作液快速达到低温状态，同时长时间保持低温，能够保持工作液内的酶的活性，保证实验的成功率，避免反复实验。

Description

一种生物实验用冰袋及其使用方法 技术领域
本发明属于分子生物学技术领域，具体涉及一种生物实验用冰袋及其使用方法。
背景技术
生物实验中有许多实验需要在低温状态下进行，例如PCR、酶切实验，特别是酶切实验中的内切酶对温度特别敏感，容易失活，在做酶切实验时，为了保持低温状态，常用的方法是取一个冰袋，将离心管架放置在冰袋上，离心管放置在离心管架上，然后将内切酶与buffer以及水按一定比例配成工作液，因为会放水比较多，所以尽管原先酶液存放在低温状态，配制成的工作液的温度，也不会立刻低下来，当做少量的实验时只需要用1.5ml或者2ml的离心管即可，一个冰袋也能够使该离心管内的工作液快速达到低温状态，同时保持该离心管内的低温状态，但是当大批量的样品时，需要配制的工作液的量比较多，往往会使用15ml，甚至50ml的离心管用来存放工作液，这时若还是将该离心管直接放置在离心管架上，会使得离心管内的工作液需要一段时间后，才能达到低温状态，在这一段时间内，位于离心管内的上部的酶可能会有部分失活，影响实验结果，甚至可能造成实验重新开始，也有人直接将离心管直接躺在冰袋上，等其降温，这势必又会增加实验操作时间。
因此急需一种能够解决以上问题的生物实验用冰袋及其使用方法。
发明内容
本发明的目的是提供一种生物实验用冰袋及其使用方法，能够快速的使离心管尤其是15ml或50ml的离心管内的工作液快速达到低温状态，同时长时间保持低温，能够保 持工作液内的酶的活性，保证实验的成功率，避免可能的反复实验。
本发明提供了如下的技术方案：
一种生物实验用冰袋，包括用于放置离心管架的主冰袋，所述主冰袋的边缘处铰接有能够绕所述主冰袋360度翻转的副冰袋，所述主冰袋和所述副冰袋均是由长方体形的内部装有内容物的塑料盒体构成，所述主冰袋上铰接有若干能够伸缩的支架，所述副冰袋上设有若干凹槽，所述凹槽沿从与所述主冰袋铰接的一端向远离所述主冰袋的一端的方向排列，所述凹槽内设有弹性圈，所述支架的一端的端部活动插入所述凹槽内并与所述弹性圈紧密相贴。当离心管比较大的时候，放置在所述主冰袋上，可以接受除了主冰袋之外的副冰袋的低温传递，在所述主冰袋和所述副冰袋之间形成的容纳离心管架的容纳空间，相比只有一个所述主冰袋而言，散热慢，能够使放置在所述主冰袋上的所述离心管架上的所述离心管内的工作液快速达到低温状态，且能够长时间保持低温状态，能够保持工作液内的酶的活性，保证实验的成功率，避免可能的反复实验。
优选的，所述主冰袋的四侧的边缘处均设有所述副冰袋。可以使放置在离心管架上的离心管接受到5个方向的冷气传输，从而使其维持在一个低温状态。
优选的，与所述副冰袋铰接的所述主冰袋的侧部设有枢轴，所述副冰袋的两端设有凸块，所述凸块上设有通孔，所述枢轴的两端分别插入两个所述通孔内。这是一种铰接方式，所述副冰袋绕着所述枢轴旋转从而调节和所述主冰袋的位置关系，从而调节放置离心管架的容纳空间的开口的大小。
优选的，所述枢轴的内部为空心，所述枢轴的空腔内装有所述内容物。所述枢轴也有冰袋的作用，从而进一步保持低温。
优选的，所述主冰袋与一个所述副冰袋之间设有1个、2个或多个所述支架。
优选的，所述内容物为高分子聚合物、吸水树脂或者水。
一种使用权利生物实验用冰袋的使用方法，其特征在于，包括以下步骤：
S1、将离心管架放在所述主冰袋的正中间；
S2、根据步骤S1中的所述离心管架的大小调节所述主冰袋和所述副冰袋之间的位置关系，从而调节所述主冰袋和所述副冰袋之间形成的容纳空间的开口大小，当所述离心管架小时，所述支架的一端插入远离所述主冰袋和所述副冰袋铰接端的所述凹槽内，当所述离心管架大时，所述支架的一端插入靠近所述主冰袋和所述副冰袋铰接端的所述凹槽内；
S3、当冰袋使用结束后，收缩所述支架，将所述支架与所述主冰袋的一个侧面相贴，将所述副冰袋翻转，使得所述副冰袋的一个侧面与所述主冰袋的一个侧面相贴，与所述副冰袋相贴的所述主冰袋的一面和设有所述支架的所述主冰袋的一面相互平行，放入冰箱内冷藏，待用。这样收藏冰袋可以减少冰袋占用的存放空间。
本发明的有益效果是：相比只有一个主冰袋而言，能够快速的使离心管尤其是15ml或50ml的离心管内的工作液快速达到低温状态，同时长时间保持低温，能够保持工作液内的酶的活性，保证实验的成功率，避免反复实验。
附图说明
附图用来提供对本发明的进一步理解，并且构成说明书的一部分，与本发明的实施例一起用于解释本发明，并不构成对本发明的限制。在附图中：
图1是本发明结构示意图；
图2是副冰袋的机构示意图；
图3是本发明的一种使用状态的俯视图。
其中图中标记为：1、主冰袋；2、副冰袋；3、支架；4、凹槽；41、弹性圈；5、枢轴；6、凸块；61、通孔；7、离心管架；8、离心管。
具体实施方式
如图1-3所示，一种生物实验用冰袋，包括用于放置离心管架7的主冰袋1，主冰袋1的边缘处铰接有能够绕主冰袋1进行360度翻转的副冰袋2，主冰袋1的四侧的边缘处均设有副冰袋2，主冰袋1和副冰袋2均是由长方体形的内部装有内容物的塑料盒体构成，内容物为高分子聚合物、吸水树脂或者水。主冰袋1上铰接有若干能够伸缩的支架3，副冰袋2上设有若干凹槽4，凹槽4沿从与主冰袋1铰接的一端向远离主冰袋1的一端的方向排列，凹槽4内设有弹性圈41，支架3的一端的端部活动插入凹槽4内并与弹性圈41紧密相贴。当离心管8比较大的时候，放置在主冰袋1上，可以接受除了主冰袋1之外的副冰袋2的低温传递，在主冰袋1和副冰袋2之间形成的容纳离心管架7的容纳空间，相比只有一个主冰袋1而言，可以使得放置在离心管架7上的离心管8接受到5个方向的冷气传输，从而使其维持在一个低温状态，散热慢，能够使得放置在主冰袋1上的离心管架7上的离心管8内的工作液快速达到低温状态，且能够长时间保持低温状态，能够保持工作液内的酶的活性，保证实验的成功率，避免可能的反复实验。
具体的，与副冰袋2铰接的主冰袋1的侧部设有枢轴5，副冰袋2的两端设有凸块6，凸块6上设有通孔61，枢轴5的两端分别插入两个通孔61内。这是一种铰接方式，副冰袋2绕着枢轴5旋转从而调节和主冰袋1的位置关系，从而调节放置离心管架7的容纳空间的开口的大小。枢轴5的内部为空心，枢轴5的空腔内装有内容物。枢轴5也有冰袋的作用，从而进一步保持低温。
具体的，主冰袋1与一个副冰袋2之间设有1个、2个或多个支架3。
一种使用生物实验用冰袋的使用方法，包括以下步骤：
S1、将离心管架7放在主冰袋1的正中间；
S2、根据步骤S1中的离心管架7的大小调节主冰袋1和副冰袋2之间的位置关系，从而调节主冰袋1和副冰袋2之间形成的容纳空间的开口大小，当离心管架7小时，支架3的一端插入远离主冰袋1和副冰袋2铰接端的凹槽4内，当离心管架7大时，支架3的一端插入靠近主冰袋1和副冰袋2铰接端的凹槽4内；也可以通过只调节支架3的长度，而不改变支架3与凹槽4的相对位置来改变主冰袋1和副冰袋2之间的位置关系。
S3、当冰袋使用结束后，收缩支架3，将支架3与主冰袋1的一个侧面相贴，将副冰袋2翻转，使得副冰袋2的一个侧面与主冰袋1的一个侧面相贴，与副冰袋2相贴的主冰袋1的一面和设有支架3的主冰袋1的一面相互平行，放入冰箱内冷藏，待用。
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

Claims (7)

  1. 一种生物实验用冰袋，其特征在于，包括用于放置离心管架的主冰袋，所述主冰袋的边缘处铰接有能够绕所述主冰袋360度翻转的副冰袋，所述主冰袋和所述副冰袋均是由长方体形的内部装有内容物的塑料盒体构成，所述主冰袋上铰接有若干能够伸缩的支架，所述副冰袋上设有若干凹槽，所述凹槽沿从与所述主冰袋铰接的一端向远离所述主冰袋的一端的方向排列，所述凹槽内设有弹性圈，所述支架的一端的端部活动插入所述凹槽内并与所述弹性圈紧密相贴。
  2. 根据权利要求1所述的生物实验用冰袋，其特征在于，所述主冰袋的四侧的边缘处均设有所述副冰袋。
  3. 根据权利要求2所述的生物实验用冰袋，其特征在于，与所述副冰袋铰接的所述主冰袋的侧部设有枢轴，所述副冰袋的两端设有凸块，所述凸块上设有通孔，所述枢轴的两端分别插入两个所述通孔内。
  4. 根据权利要求3所述的生物实验用冰袋，其特征在于，所述枢轴的内部为空心，所述枢轴的空腔内装有所述内容物。
  5. 根据权利要求1所述的生物实验用冰袋，其特征在于，所述主冰袋与一个所述副冰袋之间设有1个、2个或多个所述支架。
  6. 根据权利要求1所述的生物实验用冰袋，其特征在于，所述内容物为高分子聚合物、吸水树脂或者水。
  7. 一种使用权利要求1至6任意一项所述的生物实验用冰袋的使用方法，其特征在于，包括以下步骤：
    S1、将离心管架放在所述主冰袋的正中间；
    S2、根据步骤S1中的所述离心管架的大小调节所述主冰袋和所述副冰袋之间的位置关系，从而调节所述主冰袋和所述副冰袋之间形成的容纳空间的开口大小，当所述离心管架小时，所述支架的一端插入远离所述主冰袋和所述副冰袋铰接端的所述凹槽内，当所述离心管架大时，所述支架的一端插入靠近所述主冰袋和所述副冰袋铰接端的所述凹槽内；
    S3、当冰袋使用结束后，收缩所述支架，将所述支架与所述主冰袋的一个侧面相贴，所述副冰袋翻转，使得所述副冰袋的一个侧面与所述主冰袋的一个侧面相贴，与所述副冰袋相贴的所述主冰袋的一面和设有所述支架的所述主冰袋的一面相互平行，放入冰箱内冷藏，待用。
PCT/CN2017/111674 2017-11-17 2017-11-17 一种生物实验用冰袋及其使用方法 WO2019095286A1 (zh)

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JP2008285185A (ja) * 2007-05-16 2008-11-27 Mitsubishi Plastics Ind Ltd 保冷容器
CN203638415U (zh) * 2013-11-04 2014-06-11 深圳华大基因研究院 冰盒
CN204568405U (zh) * 2015-05-06 2015-08-19 无锡市第二人民医院 一种低温保存试管架转运盒
CN205724994U (zh) * 2016-04-21 2016-11-23 苏州慧得赛物联网技术有限公司 一种多功能移动电源装置
CN106697489A (zh) * 2015-11-17 2017-05-24 财团法人工业技术研究院 折叠式保冷箱
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JP2008285185A (ja) * 2007-05-16 2008-11-27 Mitsubishi Plastics Ind Ltd 保冷容器
CN203638415U (zh) * 2013-11-04 2014-06-11 深圳华大基因研究院 冰盒
CN204568405U (zh) * 2015-05-06 2015-08-19 无锡市第二人民医院 一种低温保存试管架转运盒
CN106697489A (zh) * 2015-11-17 2017-05-24 财团法人工业技术研究院 折叠式保冷箱
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