WO2019022300A1

WO2019022300A1 - Integrated equipment for automatic gene identification

Info

Publication number: WO2019022300A1
Application number: PCT/KR2017/011247
Authority: WO
Inventors: 이태재; 신수정; 임선영; 김지현; 신설이; 이문근; 이경균; 배남호; 이석재
Original assignee: 한국과학기술원
Priority date: 2017-07-24
Filing date: 2017-10-12
Publication date: 2019-01-31
Also published as: KR101912942B1

Abstract

The present invention relates to integrated equipment for automatic gene identification, and more specifically, to integrated equipment for automatic gene identification, the equipment allowing automatic and rapid identification of the genes of a target bacterium for analysis. The present invention provides a configuration of integrated equipment for automatic gene identification, the equipment being characterized by comprising: a main body module forming the exterior and having an integrated chip for automatic gene identification mounted in an upper portion thereof; an upper module provided above the main body module and provided to cover an upper portion of the integrated chip for automatic gene identification when rotated forward; a flow module coupled to the upper module and provided to control the flow of a fluid inside the integrated chip for automatic gene identification; an amplification module provided in the main body module and provided under an amplifier of the integrated chip for automatic gene identification; an identification module provided above the main body module and connected to an electrode unit of the integrated chip for automatic gene identification; and a control module provided in the main body module, wherein the identification module measures the residual amount of a mixture to identify a gene.

Description

Integrated automatic gene discrimination facility

The present invention relates to an integrated automatic gene discrimination facility, and more particularly, to an integrated automatic gene discrimination facility capable of automatically discriminating the gene of a detection target organism automatically.

In recent years, studies on nano-biotechnology capable of directly confirming and manipulating biomolecule behavior of nano units such as genes, proteins and cells of an object have been actively conducted. Such nano-biotechnology can be used to diagnose a disease or to determine the type of a target object, and thus the recent industrial field is being expanded.

Particularly, in recent years, the development of a lab-on-a-chip has been actively carried out. A lab-on-a-chip is a type of biochip that allows a small chip to perform research that can be done in a laboratory. Specifically, the lab-on-a-chip is made of plastic, glass, silicon, etc. to create microchannels of nanometer (nm) or less. Through this, labs and researches that can be done in existing laboratories It is a chip made to replace. Because of this practicality, lab-on-a-chip is continuously being developed and used.

However, in general, in the case of a lab-on-a-chip, the bacteria are desorbed, the bacteria are concentrated, the gene is extracted from the bacteria, and the experiment is performed by injecting the gene extracted in the lab-on-a-chip. At this time, conventionally, there has been a problem that it is necessary to separately perform the steps of concentrating the defective bacteria and extracting the genes, and then injecting the genes individually into the lab-on-a-chip.

In other words, it is difficult to carry a gene discrimination device in the past, and since it takes a long time to prepare for gene discrimination, rapid experimentation is impossible and practicality is low.

And, a typical lab-on-a-chip mixes a PCR buffer and a gene to amplify the gene. However, since the lab-on-a-chip is provided by laminating thin films, there is a problem that the PCR buffer injected between the thin films and the gene are difficult to be mixed quickly.

In addition, conventionally, when a mixed PCR solution containing a PCR buffer and a gene is injected into a lab-on-a-chip, a predetermined amount of human is injected into the lab-on-a-chip. Therefore, conventionally, it takes a lot of time to inject the mixed PCR solution into the lab-on-a-chip, and it is difficult to always inject a certain amount accurately, which lowers the reliability of the experiment.

In particular, the conventional gene chip is not fully automatic, and it takes a lot of time to identify the gene, which is not practical.

An object of the present invention to solve the above problems is to provide an integrated automatic gene discrimination facility capable of automatically discriminating the gene of the detection target bacteria automatically.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a method for manufacturing a genetic material, An upper module provided on the upper part of the main body module and covering the upper part of the integrated automatic gene discrimination chip when it is turned forward; A flow module coupled to the upper module, the flow module being adapted to control the flow of fluid in the integrated automatic gene discrimination chip; An amplification module provided in the main body module and provided under the amplifier of the integrated automatic gene discrimination chip; A discriminating module provided at an upper portion of the main body module and connected to an electrode unit of the integrated automatic gene discrimination chip; And a control module provided in the main body module, wherein the discrimination module discriminates a gene by measuring a current amount of a mixed substance.

In an embodiment of the present invention, the upper module includes an upper module body unit forming a main body and covering the upper part of the integrated automatic gene discrimination chip; And a hinge unit provided on one side of the upper module body unit, wherein the upper module body unit is rotatable forward and rearward to the hinge unit.

In an embodiment of the present invention, the flow module includes: a plurality of flow pump units connected to the integrated automatic gene discrimination chip to inject and suck air; And a plurality of flow valve units for controlling the flow of the fluid by opening and closing the valves in the integrated chip for fully automatic gene discrimination.

In an embodiment of the present invention, the flow pump unit includes: a first flow pump unit connected to a metering pump unit, a mixer pump unit, and a mixer pressure control unit of the integrated automatic gene discrimination chip; A second flow pump unit connected to the amplification pump unit, the extraction chamber pump unit and the extraction pressure regulation unit of the integrated automatic gene discrimination chip; And a third flow pump unit connected to the discrimination pump unit and the discrimination pressure regulating unit of the fully automatic gene discrimination integrated chip.

In an embodiment of the present invention, a first flow valve unit connected to an extraction container valve of the integrated automatic gene discrimination chip; A second flow valve unit connected to the extraction chamber valve unit and the extraction air injection valve of the integrated automatic gene discrimination chip; A third flow valve unit connected to the mixer valve unit of the integrated automatic gene discrimination chip; A fourth flow valve unit connected to the quantitative valve unit of the integrated automatic gene discrimination chip; A fifth flow valve unit connected to the amplification valve unit of the integrated automatic gene discrimination chip; And a sixth flow valve unit connected to the amplification mixing valve unit of the integrated automatic gene discrimination chip.

In an embodiment of the present invention, the flow pump unit comprises: a fluid delivery tube forming a body and coupled to the upper module; And a pressure regulating tube provided on one side of the fluid transfer tube, wherein the pressure regulating tube is configured to regulate the flow of the fluid between the pump and the fluid transfer tube when the fluid transfer tube is connected to the pump of the fully automatic gene- To the outside.

In an embodiment of the present invention, the flow pump unit comprises: a fluid delivery body forming a body and coupled to the upper module; An air discharge hole formed in the fluid transfer body; A fluid delivery tube having one end connected to a rotary valve provided in the main body module and the other end connected to a pump of the integrated automatic gene discrimination chip; And a sealing member provided so as to be rotatable forward and rearward and fixed to seal the air discharge hole when rotating backward.

In the embodiment of the present invention, the air discharge hole may discharge the air between the inner surface of the other end of the fluid transfer tube and the outer surface of the pump to the outside when the other end of the fluid transfer tube is connected to the pump And the like.

In an embodiment of the present invention, the amplification module may include: an amplification module main unit provided in the main body module and coupled to a position corresponding to the amplifier; A heater unit coupled to the amplification module main unit; A heat sink unit provided below the heater unit; And a fan unit coupled to one side of the heat sink unit, wherein the amplification module repeatedly raises and lowers the temperature of the amplifier to perform PCR of a gene.

In an embodiment of the present invention, the discriminating module may include a pogo pin array body provided at an upper portion of the main body module and provided at a position adjacent to the electrode unit of the integrated automatic gene discrimination chip to form a body; A pogo pin array lifting body coupled to one side of the pogo pin array body so as to be able to move up and down, and the other side of which extends toward an upper portion of the electrode unit; And a pogo pin provided on the other side of the pogo pin array lifting body for measuring an amount of current in contact with the electrode unit, wherein the pogo pin array lifting body is lifted and lowered vertically so that the pogo pin and the electrode unit are in contact with each other .

In order to achieve the above object, the present invention provides a gene discrimination apparatus using an integrated automatic gene discrimination facility.

In order to achieve the above object, the present invention provides a food poisoning discriminator using an integrated automatic gene discrimination facility.

In order to accomplish the above object, the present invention provides a fish species discriminator to which a fully automatic gene discrimination integration facility is applied.

The effect of the present invention according to the above configuration is that the bacteria to be detected can be easily concentrated using the magnetic force, and the bacteria to be detected are concentrated in two stages using the concentrating section and the extracting section, Mixing can be prevented. That is, accurate test results can be obtained.

In addition, since the process of concentrating the detection target bacteria and extracting the genes is performed automatically, it is possible to quickly and easily identify the genes.

In addition, the quantitative chamber portion, the quantitative stopper portion, and the remaining amount discharging portion of the present invention can be filled with the predetermined mixed PCR solution in each of the metering chambers without any separate control. That is, the present invention can control the mixed PCR solution injected into the amplifier to be always constant automatically, thereby improving the accuracy of the experiment, and it is economical to inject the mixed PCR solution quickly.

In addition, the quantitative air filter unit of the present invention eliminates the bubbles contained in the mixed PCR solution transferred toward the amplifier, thereby causing an error in the experimental results due to the air contained in the mixed PCR solution when the mixed PCR solution is amplified Can be prevented.

Further, the quantitative air filter unit of the present invention can prevent the mixed PCR solution stored in the amplifier from being pressurized even when the metering pump unit continuously injects air. That is, the quantitative air filter unit is convenient because the metering pump unit can precisely control the time for injecting air to transport the mixed PCR solution.

In addition, the quantitative support hole of the present invention can prevent a problem that leakage occurs when the film substrate laminated by a plurality of layers spreads when air is injected into the metering pump unit.

Further, the amplifying film portion of the amplifier of the present invention does not leak because each film is stacked so as to cover all the upper surfaces of the films laminated on the lower side.

Further, since it is not necessary to fix the probe corresponding to the gene in advance in the discriminator, the present invention shortens the preparation time for gene discrimination, and makes it possible to quickly identify the gene.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

FIG. 1 and FIG. 2 are diagrams illustrating the configuration of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

FIG. 3 is a combined perspective view of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

4 is an exploded perspective view of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

5 is an illustration of a mixer main chamber portion of a blender of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

6 is an exemplary diagram illustrating a vortex induction unit of a blender of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

7 is a diagram illustrating an internal fluid flow of a mixer main chamber part of a blender of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

8 is an exploded perspective view of an amplifier of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

9 is an exemplary diagram of a discriminator of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

FIG. 10 is a flowchart of a gene discrimination method according to an embodiment of the present invention.

Fig. 11 is a flowchart of a step of concentrating the detection target bacteria of the gene discrimination method according to an embodiment of the present invention.

FIG. 12 is a flowchart illustrating a step of generating a primary mixed solution in the step of concentrating a detection target bacterium in the gene discrimination method according to an embodiment of the present invention.

FIG. 13 is a flowchart illustrating a step of injecting a primary mixed solution of a step of concentration of a detection target bacterium in a method for identifying a gene according to an embodiment of the present invention into a concentrating part.

FIG. 14 is a flowchart illustrating a step of concentrating the detection target bacteria by discharging a foreign matter solution in the step of concentrating the detection target bacteria in the gene discrimination method according to an embodiment of the present invention.

FIG. 15 is a flowchart illustrating a step of transferring a second mixed solution in the step of concentrating the detection target bacteria in the gene discrimination method according to an embodiment of the present invention to the extraction unit.

FIG. 16 is a flowchart showing a step of re-concentrating the detection target bacteria in the step of concentrating the detection target bacteria in the gene discrimination method according to an embodiment of the present invention.

17 is a flowchart of a step of extracting a gene of the gene discrimination method according to an embodiment of the present invention.

FIG. 18 is a flowchart illustrating a step of extracting a gene from a detection subject bacterium in the step of extracting a gene of the gene discrimination method according to an embodiment of the present invention.

FIG. 19 is a flowchart illustrating a step of transferring a gene to a blender in the step of extracting a gene of the gene discrimination method according to an embodiment of the present invention.

FIG. 20 is a flowchart of a step of forming a mixed PCR solution of a gene discrimination method according to an embodiment of the present invention.

FIG. 21 is a flowchart of a step of injecting a gene according to an embodiment of the present invention.

22 is a flowchart of a step of forming a mixed material of the gene discrimination method according to an embodiment of the present invention.

FIG. 23 is a flowchart of a step of discriminating genes of a gene discrimination method according to an embodiment of the present invention.

FIG. 24 is a perspective view of an integrated module for fully automatic gene identification according to an embodiment of the present invention, in which an upper module is rotated forward; FIG.

25 is a top view of an integrated automatic gene discrimination apparatus according to an embodiment of the present invention.

26 and 27 are perspective views of the upper module of the integrated automatic gene discrimination apparatus according to the embodiment of the present invention rotated backward.

28 is a perspective view illustrating an integrated automatic gene discrimination chip mounted on a main body module of an integrated automatic gene discrimination facility according to an embodiment of the present invention.

FIG. 29 is a side perspective view of the upper module of the integrated automatic gene discrimination apparatus according to an embodiment of the present invention, which is rotated forward; FIG.

30 is a perspective view of a rotary valve unit of an integrated automatic gene discrimination integrated facility according to an embodiment of the present invention.

31 is a perspective view of the fluid pump unit of the integrated automatic gene discrimination apparatus according to another embodiment of the present invention in which the sealing member is rotated backward.

32 is a side view of the flow pump unit of the integrated automatic gene discrimination apparatus according to another embodiment of the present invention in which the sealing member is rotated forward;

FIG. 33 is a perspective view showing an upper part of a main body module in which an integrated automatic gene discrimination chip of an integrated automatic gene discrimination facility according to an embodiment of the present invention is detached. FIG.

34 is a perspective view of an amplification module of an integrated automatic gene discrimination apparatus according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as " comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 and FIG. 2 are diagrams illustrating the configuration of an integrated automatic gene discrimination chip according to an embodiment of the present invention. 3 is an assembled perspective view of an integrated automatic gene discrimination chip according to an embodiment of the present invention, and FIG. 4 is an exploded perspective view of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

1 to 4, the integrated automatic gene discrimination chip 1000 includes a concentrator 100, a mixer 200, a quantifier 300, an amplifier 400, a mixer 500, a discriminator 600 A main body chip 700, and a frame machine 800. [0035]

First, the concentrator 100 may be provided to extract a foreign object from a mixed solution in which a desalting solution and magnetic particles are mixed to concentrate the detection target bacteria, and to extract a gene in the concentrated detection target bacteria. An extracting chamber 160, an extracting air injecting unit 170, an extracting unit 180, a mixer 170, and a mixer 170. The buffer unit 120, the concentration pump unit 130, the concentration unit 140, the foreign matter discharge unit 150, And may include a transfer valve unit 190.

The mixing unit 110 is provided upstream of the thickening unit 140 to mix the desorption solution and the magnetic particles and includes a mixing vessel unit 111, a mixing heating unit 112, a mixing motor unit 113, And a mixing valve unit (114).

The mixing vessel unit 111 may be provided so that the desorption solution and the magnetic particles are injected, and the desorption solution and the magnetic particles are supported for a predetermined time. Here, the desolvation solution may be a solution which has been desorbed from food, fish, etc., but is not limited thereto. The magnetic particles may be bonded to the detection object bacteria contained in the desorption solution, and may be particles having magnetic properties.

The mixing heating unit 112 is connected to the mixing container unit 111 and can heat the mixing container unit 111 to a predetermined temperature. At this time, the mixing heating unit 112 may heat the mixing vessel unit 111 to 32 to 42 degrees so that the desorption solution and the magnetic particles can be actively mixed.

The mixing motor unit 113 may be connected to the mixing container unit 111 to apply vibration to the mixing container unit 111. Specifically, the mixing motor unit 113 may mix the desalination solution and the magnetic particles contained in the mixing vessel unit 111 by applying vibration to the mixing vessel unit 111 for 20 to 120 minutes .

Since the mixing vessel unit 111 is heated by the mixing heating unit 112 and the mixing motor unit 113 generates vibration to mix the desorption solution and the magnetic particles, The mixing efficiency of the solution and the magnetic particles can be further improved. And, when the desalination solution and the magnetic particles are mixed, the detection subject bacteria contained in the desorption solution can be combined with the magnetic particles.

The mixing valve unit 114 is provided between the mixing vessel unit 111 and the thickening unit 140 and is capable of selectively providing the mixed primary solution to the thickening unit 140 . Specifically, the mixing valve unit 114 is closed during the mixing of the desorption solution and the magnetic particles in the mixing vessel unit 111, and when the desorption solution and the magnetic particles are mixed, The mixed solution may be supplied to the concentrating part 140.

The buffer unit 120 may be provided upstream of the thickening unit 140 and the buffer unit 120 may be provided to inject the cleaning solution and the transferring solution into the thickening unit 140 have. Specifically, the buffer unit 120 may inject the cleaning solution into the thickening unit 140 so that the foreign matter solution contained in the primary mixed solution may be discharged through the foreign matter discharging unit 150. The buffer unit 120 can transfer the secondary mixed solution stored in the concentrating unit 140 to the extracting unit 180 by injecting the transferring solution into the concentrating unit 140. Here, the secondary mixed solution refers to a solution in which the foreign matter solution is discharged and concentrated in the primary mixed solution.

The concentration pump unit 130 may be disposed downstream of the mixing unit 110 and the buffer unit 120 and may be provided upstream of the concentration unit 140. The concentration pump unit 130 may inject the primary mixed solution, the cleaning solution, and the transfer solution into the concentration unit 140.

The concentrating unit 140 concentrates the detection target bacteria by discharging the foreign matter solution from the primary mixed solution in which the desorption solution and the magnetic particles are mixed, selectively generates a magnetic force, And is capable of concentrating the bacteria to be detected. The enrichment unit 140 includes a concentration vessel unit 141, a concentrated magnet unit 142, a concentration motor unit 143, a vibration motor unit 144 and a concentration valve unit 145.

The concentrating container unit 141 may be provided to receive and pass the primary mixed solution. For example, the concentrating container unit 141 may be provided in the form of a tube, and the liquid injected to one side may be discharged to the other side. However, the shape of the concentrating container unit 141 is not limited to the embodiment.

The concentrated magnet unit 142 is provided adjacent to the concentrating container unit 141 and may generate magnetic force selectively in the concentrating container unit 141. For example, the concentrated magnet unit 142 may be provided below the concentrating container unit 141 and may be provided to apply or remove the magnetic force of the concentrating container unit 141.

The condensing magnet unit 142 may be connected to the condensing motor unit 143 to adjust the distance between the condensing magnet unit 142 and the condensing container unit 141. That is, the concentrated magnet unit 142 may move adjacent to the concentrated container unit 141 by the concentrated motor unit 143 to apply a magnetic force to the concentrated container unit 141. Conversely, the condensing magnet unit 142 may be separated from the condensing container unit 141 by the condensing motor unit 143 to remove the magnetic force applied to the condensing container unit 141.

In more detail, the concentrated magnet unit 142 may have a plurality of magnets in the longitudinal direction of the concentrating container unit 141. At this time, the magnets may have different polarities of adjacent magnets. That is, the condensing magnet unit 142 may be provided such that the polarities of the magnets opposed to the concentrating container unit 141 are alternately repeated in the N and S poles.

Also, the concentrated magnet unit 142 may be electromagnetically generated to selectively generate a magnetic force in the concentrating container unit 141. In this way, when the concentrated magnet unit 142 is provided with an electromagnet, a magnetic force may be generated in the concentrated container unit 141 when a current is supplied to the concentrated magnet unit 142. On the contrary, if the current can not flow through the concentrated magnet unit 142, the magnetic force applied to the concentrated container unit 141 can be removed. When the concentrated magnet unit 142 is provided with an electromagnet, the concentrated motor unit 143 may not be provided.

In the case of the concentrated magnet unit 142 provided as an electromagnet as described above, since the magnetic force generated in the concentrating container unit 141 can be controlled by controlling the current flowing in the concentrated magnet unit 142, Easy magnetic control is possible.

The concentrated magnet unit 142 provided as described above can control the flow of the detection subject bacteria contained in the primary mixed solution by controlling the magnetic force applied to the concentrated container unit 141. [

Specifically, when the concentrated magnet unit 142 generates a magnetic force in the concentrating container unit 141, the magnetic particles combined with the detection target bacteria are moved toward the concentrated magnet unit 142 side by the magnetic force, And can be fixed to the inner wall of the container unit 141.

The vibrating motor unit 144 is connected to the concentrating container unit 141 and generates vibration in the concentrating container unit 141 when the secondary mixed solution is transferred to the extracting unit 180 And the bacteria to be detected attached to the concentrating container unit (141) are peeled off.

Specifically, the secondary mixed solution can maintain the state of being attached to the concentrated magnet unit 142 side in the concentrated vessel unit 141 even when the concentrated vessel unit 141 has its magnetic force removed. Therefore, when the vibration motor unit 144 transfers the secondary mixed solution in the concentrating container unit 141 to the extracting unit 180, Vibration can be applied to the mixed solution to separate from the inner wall. The vibration motor unit 144 provided as described above can increase the transport efficiency of the secondary mixed solution.

The foreign matter discharging unit 150 may be disposed downstream of the thickening unit 140 and the extracting unit 180 and the foreign matter discharging unit 150 may include the thickening unit 140 and the extracting unit 180 ) May be provided to discharge the foreign substance solution.

Specifically, the foreign matter discharge unit 150 includes a foreign matter outlet unit 151, a first discharge valve unit 152, and a second discharge valve unit 153.

The foreign matter outlet unit 151 is disposed on the downstream side of the concentrating unit 140 and the extracting unit 180 so that the foreign matter solution can be discharged.

The first discharge valve unit 152 is provided between the enrichment unit 140 and the discharge port unit 151. When the foreign matter solution having passed through the enrichment unit 140 is transferred to the discharge port unit 151 , And can be opened. The first discharge valve unit 152 may be closed when the second mixed solution is transferred from the enrichment unit 140 to the extraction unit 180.

The second discharge valve unit 153 is provided between the extraction unit 180 and the discharge unit 151 so that when the foreign matter solution having passed through the extraction unit 180 is transferred to the discharge unit 151 , And can be opened. The second discharge valve unit 153 may be closed when the gene extracted from the extractor 180 is transferred to the mixer 200.

The extraction chamber unit 160 can inject the extraction solution for extracting the genes in the detection target bacteria into the extraction unit 180 and the extraction chamber unit 161, the extraction chamber pump unit 162, Unit 163 and an extraction chamber pressure regulating unit 164.

The extraction chamber unit 161 may be provided to receive the extraction solution and may be provided on the upstream side of the extraction unit 180.

The extraction chamber pump unit 162 may be connected to the extraction chamber unit 161 to inject air to supply the extraction solution contained in the extraction chamber unit 161 to the extraction unit 180.

The extraction chamber valve unit 163 is provided between the extraction chamber unit 161 and the extraction unit 180 and may be provided to control whether the extraction solution contained in the extraction chamber unit 161 is supplied or not have.

The extraction chamber pressure regulating unit 164 is connected to the extraction chamber pump unit 162 and the extraction chamber pressure regulating unit 164 is connected to the fluid transfer tube for injecting air into the extraction chamber unit 161 When the extraction chamber pump unit 162 and the fluid delivery tube are connected to each other, the air between the extraction chamber pump unit 162 and the fluid delivery tube is discharged to the outside. The extraction chamber pressure regulating unit 164 thus provided is configured such that when the fluid transfer tube is connected to the extraction chamber pump unit 162, air between the extraction chamber pump unit 162 and the fluid transfer tube is introduced into the extraction chamber pump It is possible to prevent a problem of flowing into the extraction chamber unit 161 through the unit 162. That is, only a predetermined amount of air can always be injected into the extraction chamber unit 161.

The extraction chamber pressure regulating unit 164 may be opened when the extraction chamber pump unit 162 discharges the extraction solution contained in the extraction chamber unit 161 to regulate the internal pressure. The extraction chamber pressure regulating unit 164 thus provided can improve the safety by allowing the fluid transfer tube to be separated from the extraction chamber pump unit 162 while the internal pressure is adjusted.

The extraction air injection unit 170 is provided on the upstream side of the extraction unit 180 and may include an extraction air injection unit 171 and an extraction air injection valve 172.

The extraction air injection unit 171 injects air into the extraction unit 180 to discharge the gene extracted from the extraction unit 180 toward the mixer 200 provided downstream of the extraction unit 180 can do. At this time, the extraction air injection unit 171 can inject air into the extraction unit 180 in a state where a magnetic force is applied to the extraction unit 180. In this way, when the extraction unit 180 is applied with a magnetic force, the detection target bacteria and the magnetic particles are attached to the extraction unit 180 and fixed. Therefore, in a state where the extraction unit 180 is applied with a magnetic force, only the genes extracted from the detection subject organism can be transferred to the mixer 200.

When the extraction air injection unit 171 injects air into the extraction unit 180 and transfers the gene in the extraction unit 180, the extraction chamber unit 160 is also connected to the extraction unit 180 The extraction solution may be further injected to more smoothly transfer the gene.

The extracting unit 180 can re-concentrate the detection target bacteria by discharging the foreign matter solution from the secondary mixed solution in a state in which the detection target bacteria are concentrated by the concentration unit 140, The gene to be detected can be extracted. In particular, the extraction unit 180 may selectively generate a magnetic force to fix the detection target bacteria combined with the magnetic particles in the extraction unit 180, thereby re-concentrating the detection target bacteria and extracting the gene to be detected have.

Specifically, the extraction unit 180 may include an extraction container unit 181, an extraction magnet unit 182, an extraction motor unit 183, an extraction temperature control unit 184, and an extraction cooling unit 185 .

The extraction container unit 181 accommodates the detection target bacteria combined with the magnetic particles, and may be provided so that the secondary mixed solution passes through the extraction container unit 181. For example, the extraction container unit 181 may be provided in a tube shape, but the shape of the extraction container unit 181 is not limited thereto.

The extraction magnet unit 182 is provided adjacent to the extraction container unit 181 and can selectively generate magnetic force in the extraction container unit 181. For example, the extraction magnet unit 182 may be provided below the extraction container unit 181 and may be provided to apply or remove the magnetic force of the extraction container unit 181.

Specifically, the extraction magnet unit 182 may be connected to the extraction motor unit 183 to adjust the distance from the extraction container unit 181. That is, the extraction magnet unit 182 may be moved adjacent to the extraction container unit 181 by the extraction motor unit 183 to apply a magnetic force to the extraction container unit 181. Conversely, the extraction magnet unit 182 may be separated from the extraction container unit 181 by the extraction motor unit 183 to remove the magnetic force applied to the extraction container unit 181.

In more detail, the extraction magnet unit 182 may have a configuration in which a plurality of magnets are provided in the longitudinal direction of the extraction container unit 181. At this time, the magnets may have different polarities of adjacent magnets. That is, the extraction magnet unit 182 may be provided such that the polarities of the magnets opposed to the extraction container unit 181 are alternately repeated in the N and S poles. At the intersection of the N pole and the S pole, a strong magnetic flux is formed. Therefore, since the extraction magnet unit 182 captures and captures magnetic particles intensively at the point where the N pole and the S pole intersect, it is possible to prevent the problem that the detection target bacteria combined with the magnetic particles are lost.

The extraction magnet unit 182 may be electromagnetically generated to selectively generate magnetic force in the extraction container unit 181. When the extraction magnet unit 182 is provided with an electromagnet, a magnetic force may be generated in the extraction container unit 181 when a current is supplied to the extraction magnet unit 182. Conversely, if the current can not flow through the extraction magnet unit 182, the magnetic force applied to the extraction container unit 181 can be removed. If the extraction magnet unit 182 is provided with an electromagnet, the extraction motor unit 183 may not be provided.

In the case of the extraction magnet unit 182 provided as an electromagnet as described above, since the magnetic force generated in the extraction container unit 181 can be controlled by controlling the current flowing in the extraction magnet unit 182, Easy magnetic control is possible.

The extraction magnet unit 182 provided as described above can control the flow of the detection subject bacteria contained in the secondary mixed solution by controlling the magnetic force applied to the extraction container unit 181. [

Specifically, when the extraction magnet unit 182 generates a magnetic force in the extraction container unit 181, the magnetic particles combined with the detection target bacteria are moved toward the extraction magnet unit 182 by the magnetic force, And can be fixed to the inner wall of the container unit 181.

The extraction temperature control unit 184 may be connected to the extraction container unit 181 to heat and cool the extraction container unit 181 into which the extraction solution is injected to a predetermined temperature. The extraction temperature control unit 184 can heat and cool the extraction container unit 181 with the magnetic force acting on the extraction container unit 181 being removed so that the temperature of the secondary mixture solution is uniform .

The extraction container unit 181 can be heated at 90 to 100 degrees for 5 to 20 minutes. If the temperature of the extraction container unit 181 is less than 90 degrees, the extraction solution may not actively active. If the temperature of the extraction container unit 181 exceeds 100 degrees, the gene may be destroyed, The function of the solution may be lost. Thus, the extraction container unit 181 can be heated from 90 to 100 degrees for 5 minutes to 20 minutes.

As described above, the extraction solution contained in the extraction container unit 181 heated in an appropriate range can actively break down the cells of the detection target bacteria to rapidly extract genes.

However, the temperature of the extraction container unit 181 is not limited to that described above, and it may be maintained at room temperature depending on the type of extraction solution.

The extraction temperature control unit 184 can cool the heated extraction container unit 181 to room temperature. At this time, the extraction temperature control unit 184 may cool the temperature of the extraction container unit 181 by 20 to 30 degrees.

The extraction cooling unit 185 may be connected to the extraction temperature control unit 184 to cool the extraction temperature control unit 184. The extraction cooling unit 185 is adapted to be operated simultaneously when the extraction temperature control unit 184 heats the extraction container unit 181 to prevent the failure of the extraction temperature control unit 184 due to overheating And may be operated when the extraction temperature control unit 184 exceeds a predetermined temperature. The extraction cooling unit 184 can operate even when the extraction temperature control unit 184 cools the extraction vessel unit 181 to room temperature so that cooling can be performed more quickly.

The extraction unit 180 may further include a first extraction vessel valve 186 and a second extraction vessel valve 187. The first extraction vessel valve 186 and the second extraction vessel valve 187 may be provided adjacent to both sides of the extraction vessel unit 181, respectively. The first extraction vessel valve 186 and the second extraction vessel valve 187 are connected to the extraction vessel unit 181 when the extraction vessel unit 181 is heated by the extraction temperature control unit 184, It can be controlled to adjust the internal pressure and prevent the contained solution from flowing out.

The mixer transfer valve unit 190 is provided downstream of the extraction unit 180 and may be opened when the extracted gene is transferred to the mixer 200.

FIG. 5 is a diagram illustrating a mixer main chamber part of a blender of an integrated automatic gene discrimination chip according to an embodiment of the present invention, and FIG. 6 is a block diagram illustrating a vortex induction unit of a blender of an integrated automatic gene discrimination chip according to an embodiment of the present invention. And FIG. 7 is an exemplary view illustrating an internal fluid flow of a mixer main chamber portion of a mixer of an integrated automatic gene discrimination chip according to an embodiment of the present invention.

As shown in FIGS. 1 to 7, the mixer 200 is provided downstream of the concentrating extractor 100, and the gene and the PCR buffer are mixed to form a mixed PCR solution. The mixer 200 includes a mixer main chamber 210, a mixer auxiliary chamber 220, a mixer transfer chamber 230, a mixer pump 240, a mixer pressure regulator 250, And a mixer valve unit 270. The gene and the PCR buffer (polymerase chain reaction buffer) are arranged to be mixed while reciprocating between the mixer auxiliary chamber unit 220 and the mixer main chamber unit 210 . &Lt; / RTI >

The mixer main chamber portion 210 includes a mixer main chamber body 211, a mixer main chamber extension 212, a mixer injection hole 213, a mixer air filter 214 and a vortex induction unit 215, And the PCR buffer is accommodated and mixed with the gene extracted from the target bacteria.

The mixer main chamber main body 211 is provided so that the gene and the PCR buffer can be accommodated therein. Specifically, the mixer main chamber body 211 may be provided in the main body chip 700 formed by stacking a plurality of films, and a space in which the gene and the PCR buffer can be accommodated may be formed therein.

The mixer main chamber extension body 212 extends to one side of the mixer main chamber body 211 and a relatively higher step than the mixer main chamber main body 211 can be formed. The mixer main chamber extension 212 is connected to the mixer sub chamber 220 only when the mixer sub chamber 220 sucks the gene and the PCR buffer introduced into the mixer main chamber body 211. [ A step can be formed so as to be able to be transported. The mixer main chamber extension 212 may prevent the gene and the PCR buffer from moving to the mixer sub-chamber 220 when the mixer sub-chamber 220 does not apply a suction force.

The mixer injection hole 213 is formed on the bottom surface of the mixer main chamber body 211 and the extracted gene can be injected. As shown in FIG. 7A, the PCR buffer is injected from the mixer main chamber extension 212 before the gene is introduced into the mixer main chamber body 211, and is injected into the mixer injection hole 213 To the front end of the housing. That is, the mixer injection hole 213 provided in the mixer main chamber body 211 can prevent the PCR buffer pre-filled in the mixer main chamber body 211 from flowing into the mixer injection hole 213 and flowing back May be provided.

The mixer air filter 214 is provided on the upper side of the mixer main chamber body 211 and may be provided on the upper side of the mixer injection hole 213. The mixer air filter 214 mixes the gene and the PCR buffer while discharging the bubbles injected together with the gene through the mixer injection hole 213 to the outside. Specifically, the gene introduced through the mixer injection hole 213 includes bubbles. The mixer air filter 214 can discharge the bubbles introduced together with the gene to the outside. At this time, the bubble may burst and the gene and the PCR buffer may be mixed. That is, the mixer air filter 214 can improve mixing efficiency.

In addition, the mixer air filter 214 may increase the mixing efficiency as described above while discharging the bubbles generated while the gene and the PCR buffer reciprocate.

The vortex induction unit 215 may be provided in the mixer main chamber body 211 and the vortex induction unit 215 may be provided to vortex while colliding with the gene and the PCR buffer when they move . 6, the vortex induction unit 215 may be provided to extend inward from both sides of the mixer main chamber body 211, And is provided. The vortex induction unit 215 may be provided on one side of the mixer main chamber body 211 and on the other side of the mixer main chamber body 211 so as to be offset from each other.

6 (a), the vortex induction unit 215 may be provided to extend vertically inward from both sides of the mixer main chamber body 211, and as shown in FIG. 6 (b) and may be formed to extend diagonally inward from both side surfaces of the mixer main chamber body 211 as shown in Figs.

6 (d), the vortex induction unit 215 may be extended in a curved shape from both sides of the mixer main chamber body 211 toward the inside.

However, the vortex induction unit 215 is not limited to the illustrated form, and may be included in one embodiment as long as the shape and position of vortex can be actively generated when the gene and the PCR buffer move.

The vortex induction unit 215 thus provided can improve the mixing efficiency of the gene and the PCR buffer.

Since the mixer main chamber body 211 provided as described above is provided on the main body chip 700 formed by lamination of thin films, the mixing of the gene and the PCR buffer is performed in the same space, . However, the mixer main chamber part 210 according to the present invention is configured to rapidly mix the gene and the PCR buffer, so that the mixed PCR solution can be rapidly formed.

The mixer auxiliary chamber unit 220 is connected to the mixer main chamber unit 210 to receive the gene and the PCR buffer accommodated in the mixer main chamber unit 210.

The mixer transfer chamber part 230 is connected to the mixer auxiliary chamber part 220 and the mixer transfer chamber part 230 can be provided to receive a mixed PCR solution in which the gene and the PCR buffer are mixed. The mixer transfer chamber 230 may be connected to the quantitative injector 300 so that the mixed PCR solution can be discharged toward the injector 300.

The mixer pump unit 240 is connected to the mixer transfer chamber unit 230 and the mixer pump unit 240 mixes the gene and the PCR buffer with the mixer auxiliary chamber unit 220 and the mixer main chamber unit 210 to reciprocate and mix air. 7 (b), the mixer pump unit 240 sucks the mixed PCR solution contained in the mixer main chamber unit 210 and moves the mixed PCR solution to the mixer auxiliary chamber unit 220, As shown in FIG. 7 (c), air may be injected into the mixed PCR solution contained in the mixer auxiliary chamber 220 and transferred to the mixer main chamber 210. In this way, the mixer pump unit 240 can mix the gene and the PCR buffer between the mixer sub-chamber 220 and the mixer main chamber 210 and mix them.

In addition, the mixer pump unit 240 may suck the mixed PCR solution contained in the mixer auxiliary chamber unit 220 and store the mixed PCR solution in the mixer transfer chamber unit 230. The mixer pump unit 240 may be configured to inject air to discharge the mixed PCR solution contained in the mixer transfer chamber unit 230 toward the metering injector 300.

In addition, the mixer pump unit 240 may inject the PCR buffer into the mixer main chamber 210. Specifically, the mixer pump unit 240 may inject a preset amount of PCR buffer into the mixer main chamber 210 before the gene is injected.

The mixer pressure regulator 250 is connected to the mixer pump 240 and the mixer pressure regulator 250 includes a fluid delivery tube (not shown) for injecting air into the mixer pump 240, The air between the mixer pump unit 240 and the fluid delivery tube is discharged to the outside and is then sealed. The mixer pressure regulator 250 may be configured such that the air between the mixer pump unit 240 and the fluid delivery tube is supplied to the mixer pump unit 240 when the fluid delivery tube is connected to the mixer pump unit 240, Can be prevented. That is, only a predetermined amount of air can always be injected.

The mixer pressure regulator 250 may be opened when the mixer pump unit 240 discharges the mixed PCR solution to the quantitative injector 300 to regulate the internal pressure. The mixer pressure regulator 250 may be separated from the mixer pump 240 in a state in which the inner pressure of the mixer pressure regulator 250 is adjusted, thereby improving safety.

The mixer support hole 260 may be formed at a position adjacent to the mixer pump 240 and the mixer pressure regulator 250. The mixer support hole 260 may prevent the main body 700 from being deformed when the air flows into the mixer pump 240 and the mixer pressure regulator 250 . In addition, the mixer support hole 260 prevents deformation of the main body chip 700, thereby preventing leakage of water.

The mixer valve unit 270 is provided between the mixer auxiliary chamber unit 220 and the mixer transfer chamber unit 230 and includes a first mixer valve 271 and a second mixer valve 272.

The first mixer valve 271 may control the flow between the mixer auxiliary chamber part 220 and the mixer transfer chamber part 230.

The second mixer valve 272 may control the flow between the mixer transfer chamber part 230 and the metering injector 300.

The first mixer valve 271 may be configured such that the mixer pump unit 240 sucks the mixed PCR solution contained in the mixer auxiliary chamber unit 220 and transfers the mixed PCR solution to the mixer transfer chamber unit 230 It may be in an open state. At this time, the second mixer valve 272 is closed.

The second mixer valve 272 may be opened when the mixer pump unit 240 transfers the mixed PCR solution contained in the mixer transfer chamber unit 230 to the metering injector 300. At this time, the first mixer valve 271 is closed.

1 to 4, the quantitative injector 300 is provided between the mixer 200 and the amplifier 400, and can inject the mixed PCR solution in a predetermined amount into the amplifier 400 . The metering injector 300 includes a metering chamber 310, a metering stopper 320, a metering pump 340, a metering support hole 350, a metering valve 360, And a metering air filter unit 370.

The quantification chamber 310 has a plurality of quantification chambers for receiving a mixed PCR solution formed by mixing a gene and a PCR buffer. Specifically, the metering chamber 310 includes a first metering chamber 311, a second metering chamber 312, a third metering chamber 313, a fourth metering chamber 314, and a fifth metering chamber 315, . The first to third quantification chambers 311 to 315 may be provided to have a size capable of accommodating a predetermined amount of the mixed PCR solution.

The first quantification chamber 311 is provided on the upstream side to which the mixed PCR solution flows, and the downstream side of the second quantification chamber 311, the third quantification chamber 313, The first metering chamber 314 and the fifth metering chamber 315 may be sequentially arranged. The number of the metering chamber units 310 is not limited to one embodiment but may be plural.

The metering stopper 320 is provided between adjacent pair of metering chambers, and has a plurality of metering stoppers having a relatively higher stepped portion compared to the metering chambers. A first metering stopper 321 is provided between the first metering chamber 311 and the second metering chamber 312 and the second metering chamber 312 and the third metering chamber 313 are provided between the first metering chamber 311 and the second metering chamber 312, A second metering stopper 322 is provided. A third metering stopper 323 is provided between the third metering chamber 313 and the fourth metering chamber 314 and between the fourth metering chamber 314 and the fifth metering chamber 315 A fourth metering stopper 324 is provided.

The quantitative stopper unit 320 may be provided with a stepped portion for allowing the mixed PCR solution to pass therethrough in a state where the mixed PCR solution is filled in the quantitative chamber located upstream. The first quantitative stopper 321 to the fourth quantitative stopper 324 of the quantitative stopper 320 may be formed so as to form steps having the same height. In the path of the mixed PCR solution, .

For example, the introduced mixed PCR solution is first directed to the first quantitative stopper 321. However, since the first quantitative stopper 321 has a stepped height, the mixed PCR solution can not pass over the first quantitative stopper 321 and is naturally transferred to the first quantitative chamber 311, And is filled in the first metering chamber 311. When the first quantitative chamber 311 is filled with the mixed PCR solution, the water level of the mixed PCR solution rises by the step height of the first quantitative stopper 321, and the first quantitative stopper 321 It passes over. The mixed PCR solution having passed through the first quantitative stopper 321 is directed to the second quantitative stopper 322. However, since the second quantitative stopper 322 also has a stepped height, the mixed PCR solution moves toward the second quantitative chamber 312 without passing over the second quantitative stopper 322, The chamber 312 is filled. When the second quantitative chamber 312 is filled with the mixed PCR solution, the water level of the mixed PCR solution rises by the step height of the second quantitative stopper 322, and the second quantitative stopper 322 It passes over. In this manner, the third PCR chamber 313 and the fourth PCR chamber 314 can be filled with the mixed PCR solution.

In addition, since the quantitative stopper 320 is not provided in a stepped shape and the wall surface of the channel channel is made hydrophobic, the mixed PCR solution can be injected in a predetermined amount into each of the metering chambers in accordance with the difference in fluid resistance in the channel channel. .

The remaining amount discharging unit 330 may be provided to discharge the mixed PCR solution remaining after filling the metering chamber unit 310. [

The metering stopper 320 further includes a discharge stopper 325 provided between the metering chamber 310 and the residual amount discharging unit 330. Specifically, the discharge stopper 325 is provided between the fifth metering chamber 315 and the remaining amount discharging portion 330, and is relatively higher than the

metering stoppers

321, 322, 323, Can be formed. In the same manner as described above, the discharge stopper 325 may be discharged to the remaining amount discharging unit 330 through the mixed PCR solution while the mixed PCR solution is completely filled in the metering chamber 310 So that a stepped portion is formed.

The quantitative chamber 310, the quantitative stopper 320, and the residual amount discharger 330 provided as described above may be filled with a predetermined mixed PCR solution in each of the quantitative chambers without any separate control. That is, according to the present invention, the mixed PCR solution injected into the amplifier 400 can be constantly maintained, thereby improving the accuracy of the experiment, and the mixed PCR solution can be injected quickly, which is economical.

The metering pump unit 340 is provided on the upstream side of the metering chamber unit 310 and can transfer the mixed PCR solution contained in the metering chamber unit 310 to the amplifier 400. More specifically, the metering pump unit 340 is provided between the metering chamber unit 310 and the metering stopper unit 320. When the metering chamber unit 310 is filled with the mixed PCR solution, So that the mixed PCR solution contained in the quantification chamber 310 can be transferred to the amplifier 400.

The quantitative support hole 350 may be formed at a position adjacent to the quantitative pump 340. When the air is injected into the metering pump unit 340, the quantitative support hole 350 may prevent the main body chip 700 from being deformed. Also, the quantitative support hole 350 can prevent the deformation of the main body chip 700, thereby preventing the leakage of water.

The metering valve unit 360 is provided on the upstream side of the metering pump unit 340. The metering valve unit 360 may be disposed between the metering pump unit 340 and the metering stopper unit 320. The metering valve unit 360 may be disposed between the metering pump unit 340 and the metering stopper unit 320, When the mixed PCR solution contained in the metering chamber 310 is transferred to the amplifier 400, it may be in a closed state.

The quantitative air filter unit 370 is provided on the downstream side of the quantification chamber unit 310 and the quantitative air filter unit 370 is configured to collect bubbles contained in the mixed PCR solution transferred toward the amplifier 400 Can be removed. Specifically, when the amplifier 400 amplifies the gene contained in the mixed PCR solution, if the bubble is included, an error may occur in the experimental result. Therefore, the quantitative air filter unit 370 can remove bubbles contained in the mixed PCR solution transferred toward the amplifier 400, thereby preventing the above-described problems from occurring.

The quantitative air filter unit 370 may be configured to discharge all of the air injected from the quantitative pump unit 340 to stop the transfer of the mixed PCR solution when the mixed PCR solution has passed through the quantitative air filter unit 370 . Specifically, when the quantitative pump unit 340 injects air and the mixed PCR solution contained in the quantification chamber unit 310 is all transferred to the amplifier 400, the quantitative pump unit 340 is injected by the quantitative pump unit 340 The air can be exhausted through the quantitative air filter unit 370. The quantitative air filter unit 370 may prevent the mixed PCR solution contained in the amplifier 400 from being pressurized even when the metering pump unit 340 continuously injects air. Accordingly, the quantitative air filter unit 370 is not required to precisely control the injection time of the air for feeding the mixed PCR solution by the quantitative pump unit 340, which is convenient.

Referring to FIGS. 1 to 4 and 8, the amplifier 400 is provided downstream of the mixer 200, and performs PCR for the mixed PCR solution to form an amplification solution . The amplifier 400 includes an amplification film unit 410, an amplification main chamber unit 420, a first amplification valve unit 430, a second amplification valve unit 440, an amplification pump unit 450, A chamber portion 460, an amplification mixing valve portion 470, and an amplification support hole 480.

First, the amplification main chamber part 420 is provided in the amplification film part 410, and a mixed PCR solution formed by mixing a gene and a PCR buffer may be accommodated. The amplification main chamber 420 may form a channel through which the mixed PCR solution passes, and may be provided such that heating and cooling are continuously performed to generate a polymerase chain reaction.

The first amplification valve unit 430 may be provided on the amplification film unit 410 and may be provided on the upstream side of the amplification main chamber unit 420.

The second amplification valve unit 440 may be provided on the amplification film unit 410 and on the downstream side of the amplification main chamber unit 420.

The first amplification valve unit 430 and the second amplification valve unit 440 may be accommodated in the amplification main chamber 420 during the polymerase chain reaction have.

The amplification film part 410 is laminated with a plurality of films and the valve film 411 is laminated on the amplification main chamber film 412, the amplifying flow film 413, the flow path dummy film 414, the primary dummy film, A film 417, and a secondary dummy film.

The valve film 411 has the amplification main chamber part 420 at a position corresponding to the amplification main chamber part 420 formed in the main body chip 700, The first amplification valve unit 430 and the second amplification valve unit 440 may be formed on the upstream side and the downstream side, respectively.

The amplification main chamber film 412 is stacked on the valve film 411 and the amplification main chamber part 420 may be formed at a position corresponding to the valve film 411. The amplification main chamber film 412 may cover the entire upper portion of the valve film.

Specifically, the amplification main chamber film 412 includes an amplification main chamber main film 412a and a wing film 412b. The amplification main chamber part 420 may be formed in the amplification main chamber film 412 in a position and shape corresponding to the amplification main chamber part 420 formed in the valve film 411. The wing films 412b may be provided on both sides of the main amplification main body film 412a and cover the upper portions of the first amplification valve unit 430 and the second amplification valve unit 440 . At this time, the blade film 412b may be formed to have a step height higher than the amplification main chamber main film 412a.

The amplification channel film 413 is provided to cover the upper part of the amplification main chamber main film 412a and the amplification main chamber part 420 is formed at a position corresponding to the amplification main chamber main film 412a . The amplification channel film 413 may be formed to have a thickness equal to that of the amplification main chamber main film 412a and the wing film 412b. When the amplification channel film 413 is laminated on the amplification main chamber main film 412a, there is no step between the amplification channel film 413 and the wing film 412b.

The channel dummy film 414 is stacked so as to cover the entire upper portion of the amplification main chamber film 421. The channel dummy film 414 has the amplification main chamber part 420 may be formed.

The primary dummy film is stacked so as to cover the entire upper portion of the channel dummy film 414, and the amplification main chamber part 420, the first amplification valve part 430 And the second amplification valve unit 440 may be formed.

In an embodiment, the primary dummy film is composed of the first primary dummy film 415 and the second primary dummy film 415, but the primary dummy film may be provided in more than one.

The valve dummy film 417 is stacked to cover the entire upper portion of the primary dummy film, and the first amplification valve unit 430 and the second amplification valve unit 440 may be formed.

The secondary dummy film is stacked so as to cover the entire upper portion of the valve dummy film 417, and the amplification main chamber part 420, the first amplification valve part 430 And the second amplification valve unit 440 may be formed.

Further, in one embodiment, the secondary dummy film is composed of the first secondary dummy film 418 and the second secondary dummy film 419, but the secondary dummy film may be provided in more than one.

The primary dummy film and the secondary dummy film provided as described above can provide a space for swelling when the temperature of the mixed PCR solution contained in the amplification main chamber part 420 increases. That is, the dummy films provide an inflatable space of the amplification main chamber part 420 to prevent damage and sample leakage.

The amplifying film part 410 provided as described above is provided such that the films laminated on the valve film 411 cover the entire upper portion of the valve film 411 so that the liquid does not flow out, .

The amplification pump unit 450 is provided downstream of the amplification main chamber 420 and is capable of sucking and injecting air to transfer the amplified amplified solution.

The amplification auxiliary chamber part 460 is provided between the amplification pump part 450 and the amplification main chamber part 420 and can accommodate the amplification solution.

The amplification mixing valve unit 470 may be provided between the amplification assisting chamber unit 460 and the mixer 500.

The amplification support part 480 is provided at a position adjacent to the amplification pump part 450 and injects air into and discharges the amplification pump part 450. The gap of the main body chip 700 spreads, Can be prevented.

Specifically, the amplification pump unit 450 sucks air in a state in which the amplification / mixing valve unit 470 is closed, and supplies the amplification solution stored in the amplification main chamber unit 420 to the amplification auxiliary chamber unit 460 The amplification mixing valve unit 470 is opened and the amplification solution contained in the amplification auxiliary chamber unit 460 is injected into the mixer 500 ). &Lt; / RTI >

The mixer 500 is provided downstream of the amplifier 400 and may mix a signal material with the amplified solution amplified by the amplifier 400 to form a mixed material. The mixer 500 may include a mixing chamber part 510 and a mixing auxiliary chamber part 520.

The mixing chamber part 510 includes a mixing chamber main body 511, a mixing chamber extension 512, a mixing injection hole 513, a mixing air filter 514 and a vortex induction unit (not shown).

The mixing chamber chamber body 511 is provided so that the amplification solution and the signal material can be received therein. Specifically, the mixing chamber chamber body 511 may be provided in the body chip 700 formed by stacking a plurality of films, and a space in which the amplification solution and the signal material can be accommodated may be formed therein.

The mixing chamber extension body 512 is provided to extend to one side of the mixing chamber main body 511 and relatively higher steps than the mixing chamber main body 511 can be formed. Specifically, the mixing chamber extension 512 is formed in the mixing auxiliary chamber part 520 only when the amplification solution and the signal material introduced into the mixing chamber main body 511 are sucked by the mixing auxiliary chamber part 520, A stepped portion may be formed so as to be able to be transported. The mixing chamber extension 512 may prevent the amplification solution and the signal material from moving to the mixing auxiliary chamber part 520 when the mixing auxiliary chamber part 520 does not apply a suction force.

The mixing hole 513 is formed on the bottom surface of the mixing chamber main body 511 to inject the amplification solution. Like the mixer 200 shown in FIG. 7A, the signal material is injected from the mixing chamber chamber extension 512 before the amplification solution is introduced into the mixing chamber chamber body 511, And is filled up to the front end of the injection hole 513. That is, the mixing injection hole 513 provided in the mixing chamber main body 511 is positioned at a position where the signal material pre-filled in the mixing chamber chamber body 511 flows into the mixing injection hole 513, As shown in FIG.

The mixed air filter 514 is provided on the upper side of the mixing chamber main body 511 and may be provided on the upper part of the mixing injection hole 513. The mixed air filter 514 may mix the amplification solution and the signal material while discharging the bubbles injected together with the amplification solution through the mixing injection hole 513 to the outside. Specifically, the amplification solution introduced through the mixing hole 513 includes bubbles. The mixed air filter 514 may discharge the bubbles introduced together with the gene. At this time, the amplification solution and the signal material can be mixed with the bubbles. That is, the mixing air filter 514 can improve mixing efficiency.

Also, the mixing air filter 514 may discharge the bubbles generated as the amplification solution and the signal material reciprocate to the outside, thereby improving the mixing efficiency as described above.

The vortex induction unit is substantially the same as the vortex induction unit 215 of the mixer 200, so that redundant description will be omitted.

FIG. 9A is an exploded perspective view of a discriminator of an integrated automatic gene discrimination chip according to an embodiment of the present invention, and FIG. 9B is a diagram showing a discrimination of an integrated automatic gene discrimination chip according to an embodiment of the present invention Fig.

As shown in FIGS. 1 and 9, the discriminator 600 is provided downstream of the mixer 500, and can identify a gene contained in the mixed material. The discrimination device 600 can discriminate the gene by measuring the amount of the current of the mixed material and includes an electrode unit 610, a discrimination substrate unit 620, a discrimination film unit 630, a discrimination unit 640 A discrimination pump unit 650, a discrimination pressure regulating unit 660 and a discrimination support hole 670. [

The electrode unit 610 may be formed of a material such as platinum, gold, carbon, copper, nickel, or silver. The electrode unit 610 may measure the amount of current of the mixed material, and may include a working electrode 611, a counter electrode 612, And includes a reference electrode 613.

The working electrode 611 can measure the amount of current of the mixed material. Specifically, the mixed material is a mixture of the amplification solution and the signal material, and the gene contained in the amplification solution is combined with the signal material. If the number of the genes is smaller than that of the signal substance, the signal substance remains in the mixed substance in a state that the signal substance can not bind to the gene. The working electrode 611 can measure an electrical signal of the signal material that is not thus coupled. Therefore, the amount of current measured by the working electrode 611 can be changed in accordance with the amount of the signal material that is not coupled.

The counter electrode 612 can exchange electrons derived from the redox reaction of the signal material included in the mixed material with the working electrode 611. Specifically, the counter electrode 612 can exchange electrons derived from the signal material not associated with the gene of the mixed material through the redox reaction with the working electrode 611. The working electrode 611 can accurately measure the amount of current of the mixed material by measuring the amount of electrons exchanged with the counter electrode 612

The reference electrode 613 may be a reference for a voltage to be applied to the working electrode 611. The reference electrode 613 has a reference voltage for each of the mixed materials and the working electrode 611 is adapted to apply a voltage according to a reference voltage of the reference electrode 613, (611) can maintain a relatively constant voltage with respect to the mixed material.

The applied voltage applied to the working electrode 611 may be a unique voltage value for causing a redox reaction depending on each signal material included in the mixed material. In particular, the applied voltage may be adjusted in consideration of the reference electrode 613 so that the signal material included in the mixed material has a voltage value at which a redox reaction can occur.

The display voltage may be a sum of the reference voltage and the applied voltage. For example, when the reference voltage of the reference electrode 613 is 0.2V and the applied voltage to be applied to the working electrode 611 is 0.5V, the display voltage of the working electrode 611 is a reference voltage of 0.2V And an applied voltage of 0.5V.

The

electrodes

511, 512 and 513 of the electrode unit 610 include a reaction part 614 contacting the mixed material and a ground part 615 extending from the reaction part 614 and grounded ). &Lt; / RTI >

The reaction unit 614 may refer to a portion where the working electrode 611, the counter electrode 612, and the reference electrode 613 are in contact with the mixed material. The mixed material may be electrochemically reacted with the electrode unit 610 while passing through the reaction unit 614.

The ground unit 615 may refer to a grounded portion of the

electrodes

611, 612, and 613 included in the electrode unit 610 and may extend from the reaction unit 614. The grounding unit 615 may be separated from the reaction unit 614 by the discriminating film unit 630 so as not to be in contact with the mixed substance.

A plurality of the electrode units 610 may be attached to the upper portion of the identification substrate unit 620. Specifically, the material of the identification substrate unit 620 may be glass, and a plurality of the electrode units 610 may be attached to the upper portion of the identification substrate unit 620 in the longitudinal direction. At this time, the material of the identification substrate unit 620 is not limited to glass.

The discriminating film unit 630 is attached to the upper portion of the discriminating substrate unit 620, and a reaction chamber through which the mixed substance passes may be formed. Specifically, the determination film unit 630 may be provided to form the reaction chambers at positions corresponding to the plurality of electrode units 610 provided in the longitudinal direction of the identification substrate unit 620. The reaction chamber may be formed in such a shape that mixed material having passed through the mixer 200 flows into one side, reacts with the electrode unit 610, and is discharged to the other side. The width of the discriminating film unit 630 is divided into the reaction part 614 and the grounding part 615 of the electrode unit 610 so that the grounding part 615 and the mixed material are not in contact with each other .

The discriminating and discharging unit 640 may be disposed downstream of the discriminating film unit 630 and may receive the mixed substance that has passed through the discriminating film unit 630 and has been subjected to gene discrimination to be discharged to the outside.

The discrimination pump unit 650 is connected to the mixing auxiliary chamber unit 520 and the discrimination pump unit 650 amplifies the amplification solution and the signal material from the mixing chamber main body 510 and the mixing auxiliary chamber unit 520) to be mixed and reciprocated.

Specifically, the discrimination pump unit 650 sucks the air in the mixing chamber part 510 to move the amplification solution and the signal material to the mixing auxiliary chamber part 520, The amplification solution and the signal material accommodated in the chamber part 520 can be transferred to the mixing chamber part 510. As such, the discrimination pump unit 650 may mix the amplification solution and the signal material between the mixing auxiliary chamber unit 520 and the mixing chamber chamber unit 510 to form a mixed material.

The discrimination pump unit 650 may suck air such that the mixed material contained in the mixing auxiliary chamber unit 520 passes through the discriminating film unit 630 and moves to the discriminating discharging unit 640 .

The discrimination pressure regulating unit 660 is connected to the discrimination pump unit 650 and the discrimination pressure regulating unit 660 is connected to the discrimination pump unit 650 through an air suction tube (not shown) The air in the space between the discrimination pump unit 650 and the air suction tube is discharged to the outside and is then sealed. When the air suction tube is connected to the discrimination pump unit 650, the discrimination pressure regulating unit 660 provides air to the discrimination pump unit 650, It is possible to prevent the problem of flowing into the inside through the through hole.

In addition, the discrimination pressure regulating unit 660 may be opened when the discrimination pump unit 650 moves and discharges the mixed substances to regulate the internal pressure. The discrimination pressure regulating unit 660 thus provided can separate the air suction tube from the discriminating pump unit 650 while the internal pressure is adjusted, thereby improving the safety.

The discrimination support hole 670 may be formed at a position adjacent to the discrimination pump unit 650 and the discrimination support hole 670 may be formed in the main body chip 650 when the discrimination pump unit 650 sucks air. 700 can be prevented from being deformed. That is, it is possible to prevent the mixed material from flowing out due to the deformation of the main body chip 700.

The main body chip 700 is provided inside the frame unit 800 to form a body, and the injected sample can be moved. The main body chip 700 may include a main body upper film 710, a main body film 720, and a main body lower film 730, and a plurality of films may be stacked to form a channel.

The frame device 800 includes an upper frame 810 and a lower frame 820 to form an outer shape of the integrated automatic gene discrimination chip 1000. Specifically, the upper frame 810 and the lower frame 820 are coupled to each other to form an appearance of the integrated chip 1000 for an automatic gene discrimination. The frame unit 800 may be made of a plastic material, but the material of the frame unit 800 is not limited thereto.

In addition, a plurality of alignment holes may be formed in the main body chip 700, and a columnar alignment column provided in the frame unit 800 may be inserted into the alignment holes. The alignment pillars may be inserted into the alignment holes formed in the integrated automatic gene discrimination chip 1000 to fix the respective structures at predetermined positions.

The integrated automatic gene discrimination integrated chip 1000 is compact and convenient to carry. In addition, the integrated automatic gene discrimination chip 1000 is practical since it can be used immediately on the spot because the gene can be identified quickly. In addition, since the integrated chip 1000 for automatic gene discrimination does not need to fix the probe corresponding to the gene to the discriminator 600 in advance, it is possible to shorten the preparation time for gene discrimination and quickly identify the gene Do.

FIG. 10 is a flowchart of a gene discrimination method according to an embodiment of the present invention, and FIG. 11 is a flowchart of a step of concentrating a detection target bacterium of a gene discrimination method according to an embodiment of the present invention.

As shown in FIGS. 10 and 11, the gene discrimination method can first perform step S100 of concentrating the detection target bacteria. The step (S100) of concentrating the bacteria to be detected comprises a step (S110) of producing a primary mixed solution by mixing the desorption solution and the magnetic particles, a step of injecting the generated primary mixed solution into the concentration part (S120 A step S140 of discharging the foreign substance solution from the injected primary mixed solution to concentrate the detection target bacteria (S130), transferring the secondary mixed solution in the concentrated state to the extraction part (S140) And discharging the foreign substance solution from the secondary mixed solution to re-concentrate the detection target bacteria (S150).

12, in step S110 of mixing the desorption solution and the magnetic particles to form a primary mixed solution, first, a step S111 of injecting a desorption solution and magnetic particles into the mixing vessel unit is performed .

Next, a step S112 of heating the mixing vessel unit to a predetermined temperature may be performed. At this stage, the mixing vessel unit 111 can be heated to a temperature at which the mixed heating unit 112 can actively mix the desorption solution and the magnetic particles. To this end, the mixing vessel unit 111 may be heated to 32 to 42 degrees.

Next, vibration may be applied to the mixing vessel unit to mix the desorption solution and the magnetic particles to generate a primary mixed solution (S113). In this step, the mixing vessel unit 111 is subjected to vibration for 20 to 120 minutes by the mixing motor unit 113, so that the desorption solution and the magnetic particles can be mixed. In the primary mixed solution thus produced, the detection target microorganism and the magnetic particles may be combined.

Referring to FIG. 13, the step of injecting the generated primary mixed solution into the concentrating unit (S120) may be performed by first performing a step S121 of generating a magnetic force in the concentrating container unit using the concentrated magnet unit have.

Concretely, in the step S121 of generating a magnetic force to the concentrating container unit using the concentrated magnet unit, the concentrated magnet unit 142 is disposed adjacent to the concentrating container unit 141 by the concentrating motor unit 143 So that a magnetic force can be generated in the concentrating container unit 141.

Alternatively, in the step (S121) of generating a magnetic force to the concentrating container unit using the concentrated magnet unit when the concentrated magnet unit 142 is provided as an electromagnet, a current is supplied to the concentrated magnet unit 142, It is also possible to generate a magnetic force in the magnet 141.

Next, the step of injecting the primary mixed solution into the concentrating container unit using the concentrating pump unit (S122) may be performed. At this stage, the detection subject bacteria contained in the primary mixed solution may be attached to the inner wall of the concentrating container unit 141 by magnetic force in a state of being combined with the magnetic particles.

Referring to FIG. 14, the step S130 of discharging the foreign material solution from the injected primary mixed solution to concentrate the detection target bacteria is performed first (S131) of injecting the cleaning solution into the concentration portion . Prior to this step, the first discharge valve unit 152 is opened and the buffer unit 120 can inject the cleaning solution into the concentrating container unit 141.

Next, the foreign substance solution may be discharged to the foreign substance discharging unit together with the washing solution to concentrate the detection target bacteria (S132). At this stage, the cleaning solution can transfer the foreign matter solution in the primary mixed solution contained in the concentrating container unit 141 to the foreign matter outlet unit 151 and discharge it. At this time, the bacteria and the magnetic particles to be detected attached to the inner wall of the concentrating container unit 141 are not conveyed to the foreign matter outlet unit 151 together with the cleaning solution, but by the magnetic force of the concentrated magnet unit 142 By keeping the fixed state, it can be concentrated.

15, in the step S140 of transferring the secondary mixed solution in the concentrated state to the extraction unit, the magnetic force generated in the concentrating container unit is first removed, (S141) of generating a magnetic force in the unit. At this stage, the extraction magnet unit 182 may move adjacent to the extraction container unit 181 by the extraction motor unit 183 to generate a magnetic force in the extraction container unit 181.

Alternatively, in the step (S141) of removing magnetic force generated in the concentrating container unit and generating magnetic force in the extraction container unit using the extraction magnet unit when the extraction magnet unit 182 is provided as an electromagnet, the extraction magnet unit 182 to generate a magnetic force in the extraction container unit 181. [

Conversely, the concentrating unit 140 separates the concentrated magnet unit 142 from the concentrating container unit 141, or prevents current from flowing through the concentrated magnet unit 142, Can be removed.

Next, step (S142) of generating vibration in the concentrating container unit can be performed. At this stage, the vibration motor unit 144 provided in the enrichment unit 140 generates vibration in the enrichment container unit 141 to remove the detection target bacteria attached to the enrichment container unit 141 . That is, the detection target bacteria adhering to the inner wall of the thickening part 140 can be more quickly transferred to the extracting part 180.

Next, the transferring solution may be injected into the concentrating unit to transfer the secondary mixed solution to the extraction container unit (S143).

Specifically, the buffer unit 120 injects the transfer solution into the concentrating unit 140 while the enrichment valve unit 145 is opened, thereby to transfer the secondary mixed solution to the extraction container unit 181 .

Referring to FIG. 16, in step S150 of discharging the foreign matter solution from the transferred secondary mixed solution to re-concentrate the detection target bacteria, first, the detection target bacteria combined with the magnetic particles are collected in the extraction container Unit (S151) attached to the unit.

Specifically, in this step, the magnetic force generated in the concentrating container unit is removed, and the magnetic force applied to the extraction container unit 181 in the step (S 141) of generating magnetic force in the extraction container unit using the extraction magnet unit The bacteria to be detected contained in the secondary mixed solution passing through the container unit 181 can be attached to the extraction container unit 181. [

Next, the foreign substance solution transferred together with the transferring solution may be discharged to the foreign substance discharging unit, and the step of re-concentrating the detection target bacteria (S152) may be performed. Prior to this step, the second discharge valve unit 153 is switched to the open state. At this stage, the foreign matter solution other than the detection target bacteria combined with the magnetic particles may be transferred to the foreign matter discharging unit 150 together with the transferring solution and discharged. When the foreign matter solution is completely discharged through the discharge unit 150, only the detection target bacteria adhering to the inner wall of the extraction container unit 181 are left by the magnetic force, so that the re-concentration can be performed.

As shown in Figs. 10 and 17, after step (S100) of concentrating the detection subject bacteria, step (S200) of extracting genes from the concentrated detection subject bacteria can be performed.

(S200) of extracting a gene from the concentrated detection target bacteria is performed by injecting an extraction solution into an extraction unit containing the detection target bacteria combined with magnetic particles (S210), heating the extraction unit to a predetermined temperature, (S220) a step of extracting the gene from the target organism, and a step S230 of cooling the extracting unit to room temperature, and transferring the extracted gene toward the mixer (S240).

Specifically, in the step S210 of injecting the extraction solution into the extraction unit containing the detection target bacteria combined with the magnetic particles, the extraction chamber unit 160 includes the extraction unit 180 ). &Lt; / RTI > Here, the extraction solution may be a solution capable of extracting the gene in the bacteria to be detected by disrupting the bacteria to be detected.

18, a step S220 of extracting a gene from the detection target bacteria by heating the extracting unit to a predetermined temperature includes firstly removing the magnetic force applied to the extracting unit by using the extracting magnet unit (S221).

More specifically, in the step S221 of removing the magnetic force applied to the extraction unit using the extraction magnet unit, the extraction magnet unit 182 is separated from the extraction container unit 181 and is connected to the extraction unit 180 The applied magnetic force can be removed.

Alternatively, when the extracted magnet unit 182 is provided with an electromagnet, in the step S221 of removing the magnetic force applied to the extraction unit using the extracted magnet unit, the current flowing in the extracted magnet unit 182 is controlled So that the magnetic force applied to the extraction unit can be removed.

Next, the extracting unit may be heated to a predetermined temperature to extract a gene from the detection target organism (S222). At this stage, the extraction section 180 can be heated at 90 to 100 degrees for 5 to 20 minutes. If the temperature of the extraction container unit 181 is less than 90 degrees, the extraction solution may not actively active. If the temperature of the extraction container unit 181 exceeds 100 degrees, the gene may be destroyed, The function of the solution may be lost. Thus, the extraction container unit 181 can be heated from 90 to 100 degrees for 5 minutes to 20 minutes.

After the extracting unit is heated to a preset temperature and the gene is extracted from the detection target bacteria (S220), the extracting unit may be cooled to room temperature (S230). At this stage, the extraction container unit 181 can be quickly cooled by 20 to 30 degrees by the extraction temperature control unit 184 and the extraction cooling unit 185.

Referring to FIG. 19, the step of transferring the extracted gene toward a blender (S240) may first perform step S241 of applying a magnetic force to the extracting unit. At this stage, the detection target bacteria contained in the extraction container unit 181 are in a state of being fixed to the inner wall of the extraction container unit 181 by the magnetic force because they are in a state of being coupled with the magnetic particles. However, the gene extracted from the detection target bacteria is not fixed to the inner wall of the extraction container unit 181 because it is not coupled with the magnetic particles.

Next, the step of transferring the gene extracted by the extracting unit toward the blender (S242) may be performed. At this stage, the extraction air injection unit 170 may open the extraction air injection valve 172 to inject air into the extraction unit 180. At this time, the second discharge valve unit 153 is closed, and the mixer transfer valve unit 190 may be in an open state. In this state, the air injected into the extracting unit 180 may transfer the gene to the mixer 200. At this time, the bacteria to be detected attached to the extraction container unit 181 and the magnetic particles are not transferred to the mixer 200, so they can be separated from the gene and discharged naturally.

In addition, in the step S242 of transferring the gene extracted from the extracting unit toward the blender, the extraction chamber 160 further injects the extraction solution into the extraction unit 180 to smoothly transfer the gene .

10 and 20, after the step S200 of extracting the gene from the concentrated detection target bacteria, a step S300 of forming a mixed PCR solution by mixing the extracted gene with the PCR buffer can be performed . In step S300 of mixing the extracted gene with the PCR buffer to form a mixed PCR solution, step S310 of injecting a PCR buffer into the main mixer chamber may be performed. In this step, the PCR buffer is injected from the mixer main chamber extension 212 by the mixer pump unit 240 before the gene is introduced into the mixer main chamber body 211, To the front end of the housing.

Next, a step S320 of injecting a gene into the mixer main chamber portion into which the PCR buffer is injected may be performed. At this stage, the gene injected through the mixer injection hole 213 may be mixed with the PCR buffer while bubbles are discharged by the mixer air filter 214 formed in the upper part of the mixer injection hole 213 .

Next, a step S330 of injecting and sucking air such that the injected gene and the PCR buffer are mixed while reciprocating the mixer main chamber portion and the mixer auxiliary chamber portion can be performed. 7 (b), the mixer pump unit 240 sucks the mixed PCR solution contained in the mixer main chamber unit 210 and moves the mixed PCR solution to the mixer auxiliary chamber unit 220, As shown in FIG. 7 (c), air may be injected into the mixed PCR solution contained in the mixer auxiliary chamber 220 and transferred to the mixer main chamber 210. As described above, the mixer pump unit 240 may mix the gene and the PCR buffer by reciprocating the mixer auxiliary chamber unit 220 and the mixer main chamber unit 210 a plurality of times.

In addition, when the gene and the PCR buffer are mixed while reciprocating between the mixer auxiliary chamber part 220 and the mixer main chamber part 210, they may collide with the vortex induction unit 215 to generate a vortex. The resulting vortex can improve the mixing efficiency of the gene and the PCR buffer.

Next, the mixed PCR solution in which the gene and the PCR buffer are mixed can be sucked and transferred to the mixer transfer chamber part (S340). In this stage, the mixer transfer chamber unit 230 can receive and receive the mixed PCR solution contained in the mixer auxiliary chamber unit 220 by the suction force of the mixer pump unit 240.

Next, air may be injected into the mixer transfer chamber portion to transfer the mixed PCR solution to the quantitative injector (S350). At this stage, the mixer pump unit 240 may inject air to discharge the mixed PCR solution contained in the mixer transfer chamber unit 230 toward the metering injector 300.

Such a gene discrimination method improves the mixing efficiency of the gene and the PCR buffer, improves the reliability of the experimental results, and enables rapid experimentation.

10 and 21, after the step S300 of forming the mixed PCR solution by mixing the extracted gene with the PCR buffer, the mixed PCR solution thus formed may be quantitatively injected into the amplifier (S400) .

In the step S400 of injecting the mixed PCR solution formed in the amplifier (S400), the step S410 of filling the mixed PCR solution sequentially from the quantification chamber located upstream may be performed. This step may be performed automatically by the quantitative stopper having a stepped portion through which the mixed PCR solution is passed after the mixed PCR solution is filled in the quantification chamber located upstream.

Next, a step S420 of filling the remaining portion of the metering chamber and discharging the remaining mixed PCR solution to the remaining amount discharging portion may be performed. At this stage, the discharge stopper 325 (see FIG. 3), which has a stepped portion to allow the excess mixed PCR solution to pass therethrough and be discharged to the remaining amount discharge portion 330 in a state in which the mixed PCR solution is filled in the dosing chamber portion 310 ). In this case, as shown in Fig.

Next, step S430 of closing the metering valve unit may be performed.

Next, air may be injected into the metering pump unit, and the mixed PCR solution stored in the metering chamber unit may be injected into the amplifier (S440). At this stage, when the mixed PCR solution is completely filled in the quantification chamber part 310, the quantitative pump part 340 injects the mixed PCR solution stored in the quantification chamber part 310 into the amplifier (400). At this time, the quantitative air filter unit 370 can remove bubbles contained in the mixed PCR solution transferred toward the amplifier 400. When the mixed PCR solution passes through the quantitative

air filter unit

370, 340 to stop the transfer of the mixed PCR solution.

The gene discrimination method as described above can be always injected into the amplifier 400 by a predetermined amount, thereby improving the reliability of the experimental results and automatically injecting the quantitation without any additional control, The experiment can be made possible.

In addition, the PCR buffer for gene amplification may be lyophilized in a gene amplification chamber and loaded in advance.

Referring to FIG. 10, after the formed mixed PCR solution is quantitatively injected into the amplifier (S400), amplified genes are amplified by polymerase chain reaction (PCR) on the quantified mixed PCR solution to form an amplification solution (S500).

In the step S500 of amplifying the gene by PCR using the quantified mixed PCR solution (S500), the mixed PCR solution injected in the quantitative manner is amplified by the amplification of the gene Can be amplified. Here, the gene can be amplified by polymerase chain reaction (PCR). Generally, since the number of genes extracted from the detection target bacteria is very small, it is difficult to perform accurate discrimination work using only the extracted genes. However, if the number of genes is increased by amplifying the gene, the gene discrimination can be accurately performed.

Referring to FIGS. 10 and 22, after amplification of a gene through PCR (PCR) on a quantitatively-mixed PCR solution to form an amplification solution (S500), an amplification solution and a signal substance are mixed Forming a mixed material (S600) may be performed.

In the step S600 of forming the mixed material by mixing the amplification solution and the signal material, a step (S611) of injecting the amplification solution into the mixing chamber part into which the signal material is injected, and a step of injecting the amplification solution and the signal material into the mixing chamber part (S612) of injecting and sucking air to be mixed while reciprocating the mixed auxiliary chamber portion.

In this step, the signal material is injected into the mixing chamber chamber extension 512 (FIG. 5) before the amplification solution is introduced into the mixing chamber chamber body 511, at step S611, And may be filled up to the front end of the mixed injection hole 513.

Prior to the step S612 of injecting and sucking air such that the injected amplification solution and the signal substance are mixed while reciprocating the mixing chamber part and the mixing assisting chamber part, the amplification solution injected through the mixing hole 513 is mixed with the mixture The air bubbles can be mixed with the signal material while being discharged by the mixed air filter 514 formed in the upper portion of the injection hole 513. [

In addition, in the step S612 of injecting and sucking air such that the injected amplification solution and the signal substance are mixed while reciprocating the mixing chamber portion and the mixing assisting chamber portion, the discrimination pump portion 650 is connected to the mixing chamber portion 510 Air can be sucked in and the signal material and the amplification solution can be transferred to the mixing auxiliary chamber part 520. The discrimination pump unit 650 may inject air and then transfer the signal material and the amplification solution contained in the mixing auxiliary chamber unit 520 to the mixing chamber chamber unit 510. In this way, the discrimination pump unit 650 can mix the amplification solution and the signal material between the mixing auxiliary chamber part 520 and the mixing chamber part 510 a plurality of times.

Further, when the amplification solution and the signal material are mixed while reciprocating between the mixing auxiliary chamber part 520 and the mixing chamber part 510, the amplification solution and the signal material may collide with the vortex induction unit to generate a vortex. The vortex generated as described above can improve the mixing efficiency of the amplification solution and the signal material.

Referring to FIGS. 10 and 23, after the amplification solution and the signal material are mixed to form a mixed material (S600), a step S700 of determining the gene by measuring the amount of current of the mixed material may be performed .

Step S700 of determining the gene by measuring the amount of current of the mixed material formed may include applying an applied voltage to the working electrode (S710), measuring the electron derived from the redox reaction of the signal material included in the mixed material A step S720 of continuously exchanging the working electrode with the working electrode through an amount of electrons exchanged with the counter electrode S730 and a step S740 of discriminating the gene by analyzing the measured amount of current .

First, a step of applying an applied voltage to the working electrode S710 is a step of applying an applied voltage to the working electrode 611. [ Here, the applied voltage means a unique voltage value for causing a redox reaction depending on each signal material included in the mixed material. That is, by applying the applied voltage to the working electrode 611 in consideration of the reference voltage of the reference electrode 613, the redox reaction can be performed on the signal material contained in the mixed material.

In the step S720 of replacing the electrons derived from the redox reaction of the signal material included in the mixed material with the working electrode, the counter electrode 612 may oxidize the signal material contained in the mixed material Electrons derived through the reduction reaction can be exchanged with the working electrode 611. At this time, in the step of replacing the electrons derived through the redox reaction of the signal material included in the mixed material with the working electrode (S720), the signal material causing the redox reaction may be a signal material Quot; Specifically, the binding amount of the signal material is determined according to the amount of the gene, and the signal material that is not coupled to the gene is extracted through the redox reaction to provide an electrical signal to the working electrode 611 .

In the step S730 of continuously measuring the amount of current through the amount of electrons exchanged with the counter electrode, the working electrode 611 sequentially changes the amount of current through the amount of electrons exchanged with the counter electrode 612 . That is, in the step S730 of continuously measuring the amount of current through the amount of electrons exchanged with the counter electrode by the working electrode, it is possible to successively measure the amount of current change according to the amount of the signal material not coupled to the gene .

In the step S740 of determining the gene by analyzing the measured amount of electric current, when the amount of current is higher than a preset reference value, it is determined that the gene is less than the reference amount. If the amount of current is lower than the predetermined reference value, Many can be judged.

For example, in the case of a food-borne pathogen discriminator using a gene discrimination method using an integrated automatic gene discrimination chip, it is judged that the gene causing food poisoning is small and the food is safe when the electric current is higher than a preset reference value . On the other hand, if the amount of electric current is lower than a preset reference value, it is judged that there are many genes causing food poisoning, and it is judged that there is a risk of food poisoning if the food is consumed.

In addition, the method for determining the gene is not limited to the food poisoning bacteria determination device. For example, the method of gene identification can be applied to a fish species discriminator.

Next, referring to the following drawings, a description will be made of an integrated automatic gene discrimination facility.

FIG. 24 is a perspective view showing a state in which the upper module of the integrated automatic gene discrimination apparatus according to the embodiment of the present invention is rotated forward, FIG. 25 is a top view of the integrated automatic gene discrimination apparatus according to an embodiment of the present invention, 26 and 27 are perspective views of the upper module of the integrated automatic gene discrimination apparatus according to the embodiment of the present invention rotated backward.

FIG. 28 is a perspective view of an integrated automatic gene discrimination chip mounted on a main body module of an integrated automatic gene discrimination apparatus according to an embodiment of the present invention. FIG. 29 is a perspective view of an integrated automatic gene discrimination apparatus according to an embodiment of the present invention. FIG. 30 is a perspective view of a rotary valve unit of an integrated automatic gene discrimination apparatus according to an embodiment of the present invention. Referring to FIG.

As shown in FIGS. 24 to 30, the fully automatic gene discrimination integration facility 2000 includes a body module 1100, an upper module 1200, a flow module 1300, an amplification module 1400, a discrimination module 1500, Module 1600. < / RTI >

The main body module 1100 may include a main body frame unit 1110 and a rotary valve unit 1120. The main body frame unit 1100 may be mounted on the integrated chip 1000. [

The main body frame unit 1110 forms the body of the main body module 1100 and the upper module 1200 can be coupled to the upper side.

The rotary valve unit 1120 may be provided inside the body frame unit 1110. The rotary valve unit 1120 is connected to a rotary pump (not shown) provided in the main body frame unit 1110 and a flow pump unit provided in the upper module 1200 to inject and suck air .

In addition, the concentrator 140 of the integrated automatic gene discrimination chip 1000 may be positioned inside the body module 1100.

The upper module 1200 is provided on the upper part of the main body module 1100 and may be provided to cover the upper part of the integrated automatic gene discrimination chip 1000 when it is turned forward. The upper module 1200 includes an upper module main body unit 1210, a hinge unit 1220, and a handle unit 1230.

The upper module body unit 1210 forms the body of the upper module 1200 and may be provided to cover the upper part of the integrated automatic gene discrimination chip 1000.

The hinge unit 1220 may be provided on one side of the upper module body unit 1210 so that the upper module body unit 1210 can be rotated forward and rearward. Here, the forward direction may refer to a direction in which the upper module main body unit 1210 covers the upper portion of the fully automatic gene discrimination integrated chip 1000. That is, the integrated autonomous gene discrimination chip 1000 can be mounted on and dismounted from the main body module 1100 while the upper module main body unit 1210 is pivoted rearward, and the upper module main body unit 1210, In this state, the gene discrimination can proceed.

The handle unit 1230 may be provided on the other side of the upper module body unit 1210 so as to easily rotate the upper module body unit 1210 forward and backward.

The flow module 1300 may be coupled to the upper module 1200 and may be provided to control fluid flow in the integrated automatic gene discrimination chip 1000. The flow module 1300 includes a plurality of

flow pump units

1350, 1370 and 1390 connected to the fully automatic gene discrimination integrated chip 1000 to inject and suck air, and a valve in the fully automatic gene discrimination integrated chip 1000 And a plurality of flow valve units (1310, 1320, 1330, 1340, 1360, 1380) for controlling the flow of the fluid by opening and closing.

The flow pump unit includes a first flow pump unit 1350, a second flow pump unit 1370, and a third flow pump unit 1390.

The first flow pump unit 1350 is connected to the quantitative pump unit 340, the mixer pump unit 240 and the mixer pressure controller 250 of the fully automatic gene discrimination integrated chip 1000, And may be coupled to the upper module 1200. The first flow pump unit 1350 may be connected to the metering pump unit 340, the mixer pump unit 240, and the mixer pressure regulator 250 to inject and suck air.

Specifically, the first flow pump unit 1350 according to one embodiment may include a first fluid delivery tube 1351, a second fluid delivery tube 1352, and a pressure control tube 1353. The first fluid delivery tube 1351 may be connected to the metering pump unit 340 to inject air and the second fluid delivery tube 1352 may be connected to the mixer pump unit 240, Can be injected and inhaled. The pressure control tube 1353 is connected to the mixer pressure regulator 250 so that when the first flow pump unit 1350 is connected to the metering pump unit 340 and the mixer pump unit 240, The internal pressure can be adjusted.

FIG. 31 is a perspective view of the flow pump unit of the integrated automatic gene discrimination integrated facility according to another embodiment of the present invention, in which the sealing member is rotated backward. FIG. And the sealing member of the flow pump unit is rotated forward.

31 and 32, in another example, the first flow pump unit 1350 includes a first fluid delivery tube 1351, a second fluid delivery tube (not shown), a fluid delivery body 1354, An air discharge hole 1355 and a sealing member 1356. [

Specifically, the fluid transfer body 1354 forms the body of the first flow pump unit 1350 and may be coupled to the upper module body unit 1210. Specifically, the fluid transfer body 1354 may be coupled to the metering pump unit 340 and the mixer pump unit 240 at corresponding positions. The first fluid transfer tube 1351 and the second fluid transfer tube may be coupled to the fluid transfer body 1354.

The air discharge hole 1355 may be formed in the fluid transfer body 1354. Specifically, when the first fluid delivery tube 1351 and the other end of the second fluid delivery tube are connected to the metering pump unit 340 and the mixer pump unit 240, The first fluid transfer tube 1351 and the inner surface of the other end of the second fluid transfer tube and the outer surface of the metering pump unit 340 and the outer surface of the mixer pump unit 240, .

More specifically, when the metering pump unit 340 and the mixer pump unit 240 are inserted and connected to the inside of the first fluid delivery tube 1351 and the second fluid delivery tube, the metering pump unit 340 And the air between the outer surface of the mixer pump part 240 and the inner surface of the second fluid delivery tube is supplied to the metering pump part 340 and the mixer pump unit 240 are pushed and moved. Accordingly, the air discharge hole 1355 is provided between the first fluid delivery tube 1351 and the amplification pump unit 340 and the air from the second fluid delivery tube and the mixer pump unit 240 to the outside It is possible to prevent the fluid from moving before the air is artificially introduced into the amplification pump unit 340 and the mixer pump unit 240 and improve the accuracy of gene discrimination.

The first fluid transfer tube 1351 and the second fluid transfer tube are connected at one end to the rotary valve unit 1120 of the body module 1100 and at the other end to the amplification pump (340) and the mixer pump unit (240), respectively.

The sealing member 1356 is rotatable forward and rearward, and may be fixed to seal the air discharge hole 1355 when rotating backward. Specifically, the sealing member 1356 may be provided on one side of the fluid transfer body 1354, and may be provided so as to pivot forward and backward.

The sealing member 1356 is configured such that the air discharge hole 1355 is formed between the outer surface of the metering pump unit 340 and the inner surface of the first fluid delivery tube 1351 and the air between the outer surface of the metering pump unit 340 and the inner surface of the mixer pump unit 240 And the inner surface of the second fluid delivery tube may be pivoted rearward to seal the air discharge hole 1355 when the air is discharged to the outside. The sealing member 1356 thus formed can prevent air from leaking through the air discharge hole 1355 when the first fluid delivery tube 1351 and the second fluid delivery tube inject air .

As described above, the first flow pump unit 1350 may be provided according to one embodiment or another embodiment.

The second flow pump unit 1370 is connected to the amplification pump unit 450, the extraction chamber pump unit 162 and the extraction pressure regulating unit 164 of the integrated automatic gene diagnosis integrated

chip

1000, 1210 < / RTI > The second flow pump unit 1370 may include a first fluid delivery tube 1371, a second fluid delivery tube 1372, and a pressure control tube 1373.

The third flow pump unit 1390 may be coupled to the upper module body unit 1210 to be connected to the discrimination pump unit 650 and the discrimination pressure regulating unit 660 of the fully automatic gene discrimination integrated chip 1000 .

The second flow pump unit 1370 and the third flow pump unit 1390 may have substantially the same configuration as the first flow pump unit 1360 and may be constructed in accordance with one embodiment or another embodiment As shown in FIG.

Referring again to FIGS. 24 to 30, the flow valve unit includes a first flow valve unit 1310, a second flow valve unit 1320, a third flow valve unit 1330, a fourth flow valve unit 1340 ), A fifth flow valve unit 1360 and a sixth flow valve unit 1380. [

The first flow valve unit 1310 may be connected to the

extraction container valves

186 and 187 of the integrated automatic gene discrimination chip 1000. When the extraction container unit 181 is heated by the extraction temperature control unit 184, the first flow valve unit 1310 thus provided adjusts the pressure inside the extraction container unit 181, The first extraction vessel valve 186 and the second extraction vessel valve 187 can be controlled to prevent the solution from leaking out.

The second flow valve unit 1320 may be connected to the extraction chamber valve unit 163 and the extraction air injection valve 172 of the integrated automatic gene discrimination chip 1000. The second flow valve unit 1320 thus provided can control the extraction chamber valve unit 163 to determine whether to supply the extraction solution stored in the extraction chamber unit 161 to the extraction unit 180 . The second flow valve unit 1320 may control the extraction air injection valve 172 for injecting air to move the fluid contained in the extraction unit 180.

The third flow valve unit 1330 may be connected to the mixer valve unit 270 of the fully automatic gene discrimination integrated chip 1000. The third flow valve unit 1300 thus provided controls the flow between the mixer sub-chamber part 220 and the mixer transfer chamber part 230 by controlling the first mixer valve 271, The mixer valve 272 may be controlled to control the flow between the mixer transfer chamber part 230 and the metering injector 300.

The fourth flow valve unit 1340 may be connected to the quantitative valve unit 360 of the fully automatic gene discrimination integrated chip 1000. When the quantitative pump unit 340 transfers the mixed PCR solution contained in the quantification chamber unit 310 to the amplifier 400, the fourth flow valve unit 1340 provided as described above is connected to the quantitative valve unit 360 Can be controlled.

The fifth flow valve unit 1360 may be connected to the

amplification valve units

430 and 440 of the integrated automatic gene discrimination chip 1000. The fifth flow valve unit 1360 may be connected to the first amplification valve unit 430 and the second amplification unit 420 so that the mixed PCR buffer is accommodated in the amplification main chamber 420 during the PCR, The valve unit 440 can be controlled.

The sixth flow valve unit 1380 may be connected to the amplification mixing valve unit 470 of the integrated automatic gene discrimination chip 1000. The sixth flow valve unit 1380 sucks the air in the state where the amplification mixing valve unit 470 is closed and supplies the amplification solution stored in the amplification main chamber unit 420 to the amplification auxiliary chamber unit 460, The amplification mixing valve unit 470 is opened and the amplification solution contained in the amplification auxiliary chamber unit 460 is injected into the mixer 460 by injecting air in a state in which the second amplification valve unit 440 is hermetically closed. The amplification and mixing valve unit 470 can be controlled so that the amplification /

FIG. 33 is a perspective view showing an upper part of a main body module in which an integrated automatic gene discrimination chip of a fully automatic gene discrimination integrated facility according to an embodiment of the present invention is detached. FIG. A perspective view of the amplification module of the integrated facility.

33 and 34, the amplification module 1400 is provided in the main body module 1100 and may be provided below the amplifier 400 of the integrated automatic gene discrimination chip 1000. The amplification module 1400 includes an amplification module main body unit 1410, a heater unit 1420, a heat sink unit 1430, and a fan unit 1440.

The amplification module main body unit 1410 forms the body of the amplification module 1400. The amplification module main body unit 1410 is coupled to the upper side of the main body module 1100. When the integrated automatic gene recognition chip 1000 is mounted on the main body module 1100, May be coupled to corresponding locations.

The heater unit 1420 is coupled to the amplification module main body unit 1410 to increase the temperature of the mixed PCR solution contained in the amplifier 400. Specifically, as the temperature of the mixed PCR solution located in the amplifier 400 is increased and decreased repeatedly, the amount of the gene contained in the mixed PCR solution is increased. The heater unit 1420 may be used when the temperature of the mixed PCR solution located in the amplifier 400 is increased by applying heat to the amplifier 400.

The heat radiating plate unit 1430 is provided under the heater unit 1420 so that when the heater unit 1420 heats the amplifier 400, the heater unit 1420 is damaged due to excessive heat .

The fan unit 1440 is coupled to one side of the heat sink unit 1430 so as to lower the temperature of the amplifier 400 and prevent heat damage to the heater unit 1420.

As described above, the amplification module 1400 repeatedly raises and lowers the temperature of the amplifier 400 to perform the PCR of the gene.

The discrimination module 1500 is provided on the main body module 1100 and is connected to the electrode unit 610 of the integrated automatic gene discrimination chip 1000 to measure the amount of current. The determining module 1500 may include a pogo pin array body 1510, a pogo pin array lifting body 1520, and a pogo pin 1530.

The pogo pin array body 1510 is coupled to a position corresponding to the discrimination hole 1314 of the upper module main body unit 1310 to form the body of the discrimination module 1500. Specifically, the pogo pin array body 1510 may be provided at a position adjacent to the electrode unit 610 of the integrated automatic gene discrimination chip 1000.

The pogo pin array lifting body 1520 may be coupled to one side of the pogo pin array body 1510 so that the pogo pin array lifting body 1520 can be raised and lowered and the other side thereof may extend toward the upper portion of the electrode unit 610. Specifically, the pogo pin array lifting body 1520 may be provided in a plate shape, and one side of the pogo pin array body 1510 may be coupled to move upward and downward to be movable. At this time, the shape of the pogo pin array lifting body 1520 is not limited to the plate shape shown. The pogo pin array lifting body 1520 can be raised and lowered so that the pogo pin 1530 and the electrode unit 610 are in contact with each other.

The pogo pin 1530 is provided on the other side of the pogo pin array lifting body 1520 and can measure the amount of current in contact with the electrode unit 610. Specifically, the pogo pin 1530 is provided on the other side of the pogo pin array lifting body 1520, and may be provided in a position and in a number corresponding to the electrode unit 610. The pogo pin 1530 may have three fins connected to the working electrode 511, the counter electrode 512 and the reference electrode 513 of the electrode unit 610, respectively. The pogo pin 1530 thus prepared can discriminate the gene by the above-described gene discrimination method.

The genetic identification of the fully integrated gene chip 1000 is discarded and replaced with a new fully automatic gene discrimination integrated chip 1000 to perform the next gene discrimination.

The integrated automatic genotyping integrated facility (2000) provided as described above can be applied to a gene discriminator, a food poisoning discriminator, a fish species discriminator, and the like.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims

A body module for mounting an integrated automatic gene discrimination chip on the upper part and forming an external shape;

An upper module provided on the upper part of the main body module and covering the upper part of the integrated automatic gene discrimination chip when it is turned forward;

A flow module coupled to the upper module, the flow module being adapted to control the flow of fluid in the integrated automatic gene discrimination chip;

An amplification module provided in the main body module and provided under the amplifier of the integrated automatic gene discrimination chip;

A discriminating module provided at an upper portion of the main body module and connected to an electrode unit of the integrated automatic gene discrimination chip; And

And a control module provided in the main body module,

Wherein the discrimination module discriminates a gene by measuring a current amount of a mixed substance.
The method according to claim 1,

The upper module includes:

An upper module main body unit forming a main body and covering an upper portion of the integrated automatic gene discrimination chip; And

And a hinge unit provided on one side of the upper module body unit,

Wherein the upper module body unit is rotatably provided to the hinge unit forward and backward.
The method according to claim 1,

The flow module comprises:

A plurality of flow pump units connected to the integrated automatic gene discrimination chip for injecting and sucking air; And

And a plurality of flow valve units for controlling the flow of the fluid by opening and closing the valves in the fully automatic gene discrimination integrated chip.
The method of claim 3,

The flow pump unit comprises:

A first flow pump unit connected to the metering pump unit, the mixer pump unit, and the mixer pressure regulator of the integrated automatic gene discrimination chip;

A second flow pump unit connected to the amplification pump unit, the extraction chamber pump unit and the extraction pressure regulation unit of the integrated automatic gene discrimination chip; And

And a third flow pump unit connected to the discrimination pump unit of the integrated automatic gene discrimination chip and the discrimination pressure regulating unit.
The method of claim 3,

A first flow valve unit connected to an extraction container valve of the integrated automatic gene discrimination chip;

A second flow valve unit connected to the extraction chamber valve unit and the extraction air injection valve of the integrated automatic gene discrimination chip;

A third flow valve unit connected to the mixer valve unit of the integrated automatic gene discrimination chip;

A fourth flow valve unit connected to the quantitative valve unit of the integrated automatic gene discrimination chip;

A fifth flow valve unit connected to the amplification valve unit of the integrated automatic gene discrimination chip; And

And a sixth flow valve unit connected to the amplification mixing valve unit of the integrated automatic gene discrimination chip.
The method of claim 3,

The flow pump unit comprises:

A fluid delivery tube forming a body and coupled to the upper module; And

And a pressure control tube provided on one side of the fluid transfer tube,

Wherein the pressure regulating tube discharges air between the pump and the fluid transfer tube to the outside when the fluid transfer tube is connected to the pump of the fully automatic gene discrimination integrated chip.
The method of claim 3,

The flow pump unit comprises:

A fluid delivery body forming a body and coupled to the upper module;

An air discharge hole formed in the fluid transfer body;

A fluid delivery tube having one end connected to a rotary valve provided in the main body module and the other end connected to a pump of the integrated automatic gene discrimination chip; And

And a sealing member provided so as to be rotatable toward the front and rear sides and fixed to seal the air discharge hole when rotating backward.
8. The method of claim 7,

The air discharge hole

And an air outlet between the inner surface of the other end of the fluid transfer tube and the outer surface of the pump when the other end of the fluid transfer tube is connected to the pump.
The method according to claim 1,

Wherein the amplification module comprises:

An amplification module main unit provided on the main body module and coupled to a position corresponding to the amplifier;

A heater unit coupled to the amplification module main unit;

A heat sink unit provided below the heater unit; And

And a fan unit coupled to one side of the heat sink unit,

Wherein the amplification module repeatedly raises and lowers the temperature of the amplifier so that PCR of the gene is performed.
The method according to claim 1,

The discrimination module comprises:

A pogo pin array body provided at an upper portion of the main body module and provided at a position adjacent to the electrode unit of the integrated automatic gene discrimination chip to form a body;

A pogo pin array lifting body coupled to one side of the pogo pin array body so as to be able to move up and down, and the other side of which extends toward an upper portion of the electrode unit; And

And a pogo pin provided on the other side of the pogo pin array lifting body for measuring an amount of current in contact with the electrode unit,

Wherein the pogo pin array lifting body is vertically moved up and down so that the pogo pin and the electrode unit are in contact with each other.