WO2019022299A3 - Fully-automatic gene-reading integrated chip - Google Patents

Fully-automatic gene-reading integrated chip Download PDF

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Publication number
WO2019022299A3
WO2019022299A3 PCT/KR2017/011246 KR2017011246W WO2019022299A3 WO 2019022299 A3 WO2019022299 A3 WO 2019022299A3 KR 2017011246 W KR2017011246 W KR 2017011246W WO 2019022299 A3 WO2019022299 A3 WO 2019022299A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
solution
amplifier
combined
integrated chip
Prior art date
Application number
PCT/KR2017/011246
Other languages
French (fr)
Korean (ko)
Other versions
WO2019022299A9 (en
WO2019022299A2 (en
Inventor
이태재
신수정
임선영
김지현
신설이
이문근
이경균
배남호
이석재
Original Assignee
한국과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국과학기술원 filed Critical 한국과학기술원
Publication of WO2019022299A2 publication Critical patent/WO2019022299A2/en
Publication of WO2019022299A3 publication Critical patent/WO2019022299A3/en
Publication of WO2019022299A9 publication Critical patent/WO2019022299A9/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • G01N35/085—Flow Injection Analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)

Abstract

The present invention relates to a full-automatic gene-reading integrated chip and, more particularly, to a full-automatic gene-reading integrated chip adapted to rapidly read a gene of bacteria to be detected, in a fully automated manner. The configuration of the present invention provides a fully-automatic gene-reading integrated chip comprising: a concentration extractor for enriching a bacteria to be detected by discharging a foreign matter solution out of a combined solution in which a detaching solution and magnetic particles are combined and for extracting a gene from the enriched bacteria to be detected; a mixer, arranged downstream of the concentration extractor, for mixing the gene and a PCR buffer to form a mixed PCR solution; an amplifier, arranged downstream of the mixer, for performing a polymerase chain reaction (PCR) with the mixed PCR solution to give an amplified solution; a quantitative injector, arranged between the mixer and the amplifier, for injecting a determined quantity of the mixed PCR solution to the amplifier; a combiner, arranged downstream of the amplifier, for combining a signaling substance with the amplification solution amplified by the amplifier to form a combined matter; and a reader, arranged downstream of the combiner, for reading the gene contained in the combined matter, wherein the reader measures a current amount of the combined matter to read the gene.
PCT/KR2017/011246 2017-07-24 2017-10-12 Fully-automatic gene-reading integrated chip WO2019022299A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0093651 2017-07-24
KR1020170093651A KR101971636B1 (en) 2017-07-24 2017-07-24 Integrated chip for automatic discrimination of gene

Publications (3)

Publication Number Publication Date
WO2019022299A2 WO2019022299A2 (en) 2019-01-31
WO2019022299A3 true WO2019022299A3 (en) 2019-03-21
WO2019022299A9 WO2019022299A9 (en) 2019-05-16

Family

ID=65040608

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/011246 WO2019022299A2 (en) 2017-07-24 2017-10-12 Fully-automatic gene-reading integrated chip

Country Status (2)

Country Link
KR (1) KR101971636B1 (en)
WO (1) WO2019022299A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022080970A1 (en) * 2020-10-15 2022-04-21 Seegene, Inc. Solution mixing device and solution mixing method
DE102021109852A1 (en) * 2021-04-19 2022-10-20 Ist Innuscreen Gmbh Method for the automated online detection of at least one biological target substance in a liquid and online analysis device

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100401389B1 (en) * 2000-10-27 2003-10-17 김희태 A method for detecting a specific nucleic acid, a kit therefor and an apparatus for driving it
KR101319492B1 (en) * 2012-02-10 2013-10-17 한국과학기술원 Electrochemical One-Step Detection Method of DNA Based on the Transport Control of Signaling Materials
KR20140071213A (en) * 2012-12-03 2014-06-11 삼성전자주식회사 Reagent container for amplification nucleic acid, method for manufacturing the same, method for storing the reagent, and micro-fluidic system for analysis of nucleic acid
KR101465961B1 (en) * 2007-10-09 2014-12-01 삼성전자주식회사 A method and a device for detecting DNAs, etc.
KR101642785B1 (en) * 2014-03-10 2016-07-28 대한민국 DNA Scanner Comparing And Analyzing Electrochemical Detecting Signal For Identifying Species Or Origin About Fish And Shellfish
KR20170012806A (en) * 2015-07-24 2017-02-03 케이맥(주) Method of extracting and amplifying nucleic acids using direct elution
KR20170024827A (en) * 2015-08-26 2017-03-08 (주)바이오니아 The Quantitative PCR Cartridge with Microchannel-Film Reactor, Nucleic Acid Extraction Module and qPCR Reagents Module, and The Rapid qPCR System Using the Same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101421098B1 (en) 2013-05-31 2014-07-18 고려대학교 산학협력단 Lap-on-a-chip for multi detection and fluid flow control

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100401389B1 (en) * 2000-10-27 2003-10-17 김희태 A method for detecting a specific nucleic acid, a kit therefor and an apparatus for driving it
KR101465961B1 (en) * 2007-10-09 2014-12-01 삼성전자주식회사 A method and a device for detecting DNAs, etc.
KR101319492B1 (en) * 2012-02-10 2013-10-17 한국과학기술원 Electrochemical One-Step Detection Method of DNA Based on the Transport Control of Signaling Materials
KR20140071213A (en) * 2012-12-03 2014-06-11 삼성전자주식회사 Reagent container for amplification nucleic acid, method for manufacturing the same, method for storing the reagent, and micro-fluidic system for analysis of nucleic acid
KR101642785B1 (en) * 2014-03-10 2016-07-28 대한민국 DNA Scanner Comparing And Analyzing Electrochemical Detecting Signal For Identifying Species Or Origin About Fish And Shellfish
KR20170012806A (en) * 2015-07-24 2017-02-03 케이맥(주) Method of extracting and amplifying nucleic acids using direct elution
KR20170024827A (en) * 2015-08-26 2017-03-08 (주)바이오니아 The Quantitative PCR Cartridge with Microchannel-Film Reactor, Nucleic Acid Extraction Module and qPCR Reagents Module, and The Rapid qPCR System Using the Same

Also Published As

Publication number Publication date
KR20190011367A (en) 2019-02-07
KR101971636B1 (en) 2019-04-24
WO2019022299A9 (en) 2019-05-16
WO2019022299A2 (en) 2019-01-31

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