WO2019008679A1

WO2019008679A1 - Simulate biological sample

Info

Publication number: WO2019008679A1
Application number: PCT/JP2017/024533
Authority: WO
Inventors: 裕基庄野; 成田　利治; 明日香向井; 遼佑伊藤
Original assignee: オリンパス株式会社
Priority date: 2017-07-04
Filing date: 2017-07-04
Publication date: 2019-01-10
Also published as: WO2019009279A1

Abstract

This simulate biological sample (1) comprises: a first member (2) that includes a fluorescent material and simulates the shape of biological tissue to be observed; and a second member (3) that covers the first member (2) and simulates the optical properties of tissue surrounding the biological tissue to be observed.

Description

Biological simulation sample

The present invention relates to a biological simulation sample, and more particularly to a biological simulation sample for fluorescence observation.

Conventionally, a method of injecting a fluorescent dye such as indocyanine green (ICG) into a patient and measuring the fluorescence intensity from the fluorescent dye in blood is known as a means for determining the presence or absence of blood flow in living tissue. There is. In this method, a standard sample that emits fluorescence of known intensity is used to standardize the measured intensity of fluorescence (see, for example, Patent Documents 1 and 2).

JP 2005-300540 A JP, 2013-96920, A

However, the standard samples disclosed in

Patent Documents

1 and 2 are not suitable for use as simulated samples for performance evaluation and demonstration of endoscopes. That is, in the performance evaluation and demonstration of the endoscope, while using a simulated sample instead of the living tissue in the living body, the appearance of the fluorescence from the living tissue in the image acquired by the endoscope in the clinical scene is as real as possible It is required to reproduce in However, since the standard samples of

Patent Documents

1 and 2 do not simulate living tissue, they can not achieve a near-visible appearance of fluorescence from living tissue in a clinical setting.

The present invention has been made in view of the above-described circumstances, and it is an object of the present invention to provide a biological simulation sample capable of realizing the appearance close to the appearance of fluorescence from biological tissue in a clinical situation.

In order to achieve the above object, the present invention provides the following means.
One aspect of the present invention is a first member containing a fluorescent material and simulating the shape of a living tissue to be observed, and a second member covering the first member and simulating the optical characteristics of the surrounding tissue of the living tissue to be observed And a biological simulation sample comprising
According to this aspect, the shape of the living tissue to be observed is simulated by the first member, and the optical property of the peripheral tissue to be observed is simulated by the second member around the first member. Therefore, by irradiating the biological simulation sample with excitation light to cause the fluorescent material contained in the first member to emit light, it is possible to realize a near-visible appearance of fluorescence from biological tissue in a clinical situation.

In the above aspect, the second member has a layer structure in which two or more layers are stacked, and the two or more layers are different from each other in at least one of the light scattering property and the light absorption property. Good.
Such a second member more realistically simulates the optical characteristics of the surrounding tissue having a layered structure. This makes it possible to achieve a view closer to the view of the living tissue to be observed in the surrounding tissue having a layered structure.

In the above aspect, the first member may simulate the shape of a vessel, for example, the running shape of a blood vessel or the shape of a lymphatic vessel.
In this way, a closer view of fluorescence from vessels such as blood vessels or lymph vessels in a clinical setting can be realized by the first member simulating the shape of the vessel.

In the above aspect, the fluorescent material may be indocyanine green (ICG).
ICG is often used for fluorescence observation of vessels in the clinic. Therefore, a closer view of the fluorescence from vessels in a clinical setting can be achieved by the first member including the ICG.

In the above aspect, the first member is made of a gel material containing a fluorescent material, the second member is made of a cured resin material, and the first member is embedded in the second member. It is also good.
By using a gel material that can be shaped into any shape, various biological tissue shapes can be simulated by the first member. In addition, by embedding the gel material in a cured resin material that is stable against air and moisture, the gel material can be prevented from drying and the biological simulation sample can be stored for a long time.

ADVANTAGE OF THE INVENTION According to this invention, it is effective in the ability to implement | achieve the appearance close | similar to the appearance of the fluorescence from the biological tissue in a clinical scene.

It is a perspective view of a living body simulation sample concerning one embodiment of the present invention. It is a side view of the biological simulation sample of FIG. 1A. It is a top view of the living body simulation sample which shows the modification of the shape of the 1st member of Drawing 1A. It is a top view of the living body simulation sample which shows the other modification of the shape of the 1st member of Drawing 1A. It is a perspective view of the modification of a living body simulation sample of Drawing 1A. It is a side view of a living body simulation sample of Drawing 4A. It is a perspective view of the other modification of the biological simulation sample of FIG. 1A.

Hereinafter, a biological simulation sample 1 according to an embodiment of the present invention will be described with reference to the drawings.
The living body simulation sample 1 according to the present embodiment is used as a subject instead of a living tissue in a living body in the evaluation and demonstration of the image performance of an endoscope. As shown in FIGS. 1A and 1B, the biological simulation sample 1 includes the first member 2 simulating the shape of the biological tissue to be observed, and the optical characteristics of the surrounding tissue of the biological tissue to be observed covering the first member 2 And a second member 3 that simulates.

The first member 2 is made of a gel material containing indocyanine green (ICG). As a gel material, for example, an agar gel, a gelatin gel, a two-component mixed gel, or the like is used. The first member 2 has a shape that simulates the traveling shape of a blood vessel to be observed. For example, the first member 2 has a branch structure in which branching from one linear structure to a plurality of linear structures is repeated. In FIG. 1A, the shape of the first member 2 is simplified.

The second member 3 is made of a block-like or sheet-like cured resin material, and the first member 2 is embedded in the second member 3. As the resin material, silicone resin, polyurethane resin, epoxy resin or the like is used. The second member 3 is the same as or similar to the surrounding tissue to be observed in at least one of the light absorption property and the light scattering property.

Such a biological simulation sample 1 is manufactured, for example, by the following method.
The gel material is uniformly mixed with ICG, the gel material is filled in a syringe, and the gel material is discharged in a thread form from the syringe, thereby forming the gel material into a shape that simulates the traveling shape of a blood vessel. Next, a liquid resin material is poured into a mold, the entire molded gel material is placed inside the resin material, and the resin material is cured. Thereby, the biological simulation sample 1 shown in FIG. 1A is manufactured.

Next, a method of using the biological simulation sample 1 configured as described above will be described.
The biological simulation sample 1 is disposed to face the tip of the endoscope, the excitation light (wavelength 760 nm) is irradiated to the biological simulation sample 1 from the endoscope, and the fluorescence (wavelength) emitted from the ICG contained in the first member 2 830 nm) is observed by an endoscope. Thereby, an image (simulated image) simulating an image obtained by imaging fluorescence from a blood vessel in a living body to which ICG has been administered in a clinical scene is acquired by the endoscope. The acquired simulated image of the biological simulation sample 1 is displayed on the display. The user evaluates the imaging performance of the endoscope during clinical use based on the simulated image displayed on the display.

In this case, according to the present embodiment, since the first member 2 simulates the traveling shape of the blood vessel in the living body, in the living body simulation sample 1, fluorescence is emitted in the same shape as the traveling shape of the blood vessel. Furthermore, since the second member 3 around the first member 2 simulates the optical properties of the surrounding tissue of the blood vessel, the fluorescence emitted from the first member 2 is the same as in living tissue in the second member 3 Spread out. As a result, in the simulated image of the biological simulation sample 1, the appearance close to the appearance of the fluorescence observed by the endoscope in the clinical scene is realized. Thus, the user can accurately evaluate the appearance of fluorescence in the image when the endoscope is used clinically based on the simulated image on the display.

In addition, since the first member 2 is made of a gel material that can be formed into an arbitrary shape, the shape of any living tissue including a living tissue having a complicated shape such as a traveling shape of a blood vessel Has the advantage of being able to simulate
In addition, although the gel material constituting the first member 2 is not suitable for long-term storage since the characteristics change due to drying etc., the cured resin material constituting the second member 3 is stable against air and moisture. Material and suitable for long-term storage. Therefore, covering the entire first member 2 with the second member 3 has an advantage of being able to provide the biological simulation sample 1 that can be stored for a long time while maintaining the quality of the fluorescent material.

In the present embodiment, the first member 2 simulates the traveling shape of the blood vessel. However, instead of this, the shape of another living tissue may be simulated.
For example, as shown in FIG. 2, the first member 2 may simulate the shape of a lymphatic vessel and may simulate the shape of another vessel such as a bile duct. Alternatively, as shown in FIG. 3, the first member 2 may simulate the shape of a tumor.

In the present embodiment, ICG is used as the fluorescent material, but the type of fluorescent material is not limited to this, and other types of fluorescent materials may be used. For example, quantum dots or protoporphyrin IX (ppIX) may be used as the fluorescent material.
In the clinic, protoporphyrin IX (ppIX) is often used for fluorescent labeling of tumor tissue such as cancer. Therefore, it is preferable to use ppIX for the first member 2 that simulates the shape of the tumor in FIG. 3 so as to achieve a closer look to the fluorescence from the tumor in the clinical setting.

In the present embodiment, although the single first member 2 is disposed in the second member 3 having a single-layer structure, the number of first members 2 and the number of layers constituting the second member 3 Can be changed as appropriate.
For example, as shown in FIGS. 4A and 4B, the plurality of

first members

21 and 22 may be disposed at mutually different depths in the second member 3 having a single-layer structure. The shapes of the plurality of

first members

21 and 22 may be identical to each other, or may be different from each other as shown in FIG. 4A.

Further, as shown in FIG. 5, the second member 3 may have a layered structure in which two or

more layers

3 a and 3 b are stacked. The 2nd member 3 of 2 layer structure is shown by FIG. 5 as an example.
Surrounding tissues such as blood vessels, lymph vessels, and tumors have a layered structure composed of multiple layers having different light scattering properties and light absorption properties. The

layers

3a and 3b of the second member 3 are different from each other in at least one of the light scattering property and the light absorption property so as to simulate the optical property of each layer (for example, the mucous layer and muscle layer) of the surrounding tissue. . This makes it possible to achieve an appearance closer to the appearance of fluorescence from the observation target in a clinical setting.

Specifically, the

layer

3a, 3b have respectively about 0.1 mm ^-1 or more ^{5 mm -1} or less of the light scattering coefficient in the wavelength range of visible light. The

layers

3a and 3b have light absorption characteristics that simulate the absorption spectrum of blood, and each have a light absorption coefficient of more than about 0 mm ^-1 and 20 mm ^-1 or less in the visible light wavelength range. The light scattering coefficient and the light absorption coefficient of the layer 3b simulating the layer on the deep side are preferably larger than the light scattering coefficient and the light absorption coefficient of the layer 3a simulating the layer on the front surface, respectively.

When the second member 3 has a plurality of

layers

3a and 3b as shown in FIG. 5, the first member 2 may be disposed only in one layer, and is disposed between two adjacent layers. It may be provided, or may be disposed across multiple layers. Alternatively, the first member 2 may be disposed in each of the

layers

3a and 3b.

1 living

body simulation sample

2, 21, 22 first member 3

second member

3a, 3b layer

Claims

A first member containing a fluorescent material and simulating the shape of a living tissue to be observed;
And a second member that covers the first member and simulates the optical characteristics of the surrounding tissue of the living tissue to be observed.
The second member has a layer structure in which two or more layers are stacked,
The biological simulation sample according to claim 1, wherein the two or more layers differ from each other in at least one of light scattering properties and light absorption properties.
The biological simulation sample according to claim 1 or 2, wherein the first member simulates the shape of a vessel.
The biological simulation sample according to claim 3, wherein the first member simulates a traveling shape of a blood vessel or a shape of a lymphatic vessel.
The biological simulation sample according to any one of claims 1 to 4, wherein the fluorescent material is indocyanine green.
The first member is made of a gel material containing a fluorescent material,
The second member is made of a cured resin material,
The biological simulation sample according to any one of claims 1 to 5, wherein the first member is embedded in the second member.