WO2018159055A1

WO2018159055A1 - Fluorescence assay multi-well plate and multi-well plate set, and method for producing fluorescence assay multi-well plate

Info

Publication number: WO2018159055A1
Application number: PCT/JP2017/044154
Authority: WO
Inventors: 秀和内田; 岩渕　康男
Original assignee: 信越ポリマー株式会社
Priority date: 2017-02-28
Filing date: 2017-12-08
Publication date: 2018-09-07
Also published as: JP2018141693A; TW201833556A

Abstract

[Problem] To make it easy to handle smaller well capacities and easy to transfer samples from one multi-well plate to another multi-well plate. [Solution] A fluorescence assay multi-well plate 1 provided with a plurality of wells 15 for use in a fluorescence assay on a sample D, wherein: the fluorescence assay multi-well plate is provided with a multi-hole plate 10 provided, along the thickness direction of the plate, with a plurality of through holes 16 corresponding to the plurality of wells 15, and a transparent film 20 for closing off one opening surface side of the through holes 16 in the multi-hole plate 10; the multi-hole plate 10 is formed by dispersing a light-blocking filler 6 into a rubber-made elastic body 5; and the film 20 and the multi-hole plate 10 are fixed via a transparent pressure-sensitive adhesive layer 30. The invention also pertains to a multi-well plate set 2 and a method for producing a fluorescence assay multi-well plate.

Description

Multiwell plate and multiwell plate set for fluorescence measurement, and manufacturing method of multiwell plate for fluorescence measurement

Cross reference

This application claims priority based on Japanese Patent Application No. 2017-035694 filed in Japan on February 28, 2017, the contents of which are incorporated herein by reference. Moreover, the content described in the patent quoted in this application, a patent application, and literature is used for this specification.

The present invention relates to a fluorescence measurement multiwell plate for fluorescence measurement of a large number of samples, a multiwell plate set that can be used in combination with a pair, and a method for producing a fluorescence measurement multiwell plate.

Conventionally, simultaneous fluorescence measurement of a large number of samples using a multiwell plate is known as a technique representative of the initial screening of a drug discovery process. The conventional multiwell plate has a large capacity for each well, and is suitable for performing a sample sorting operation using a manual or automatic multiple pipette or the like. However, multi-well plates (Multi Micro Vessel: MMV) in which a larger number of wells with smaller capacities are arranged have been developed recently as the amount of samples is reduced and the efficiency of measurement increases.

By the way, a method is known in which a sample filled in the well of the first MMV is transferred to the second MMV at a time so that the sorting operation of the first MMV can be performed easily and in a short time. More specifically, the opening side of the well of the first MMV and the opening side of the well of the second MMV face each other, and an isolation sheet (with a flow hole) is sandwiched between them, and the second MMV to the second A centrifugal force is applied in the direction of the MMV of the first MMV, and the sample in the well of the first MMV is transferred into the well of the second MMV through the flow hole of the isolation sheet (see Patent Document 1). According to such a method, it is possible to transfer from one MMV containing a large number of samples to another MMV all at once, and liquid leakage hardly occurs during the transfer.

JP 2013-195370 A

The conventional MMV is made of a thermoplastic resin, and in many cases, a black filler is dispersed in the resin in order to prevent light leakage between adjacent wells. However, it has become difficult for thermoplastic resins to cope with the reduction in the well capacity. In addition, some users desire a method that does not use an isolation sheet when transferring a sample between two MMVs at once.

The present invention has been made in view of the above situation, and it is an object of the present invention to easily cope with a reduction in the volume of a well and to easily transfer a sample from one multiwell plate to another multiwell plate. And

(1) A fluorescence measurement multi-well plate according to an embodiment for achieving the above object is a fluorescence measurement multi-well plate having a plurality of wells for use in fluorescence measurement of a sample. A multi-hole plate having a plurality of through holes corresponding to the plate along the thickness direction of the plate, and a transparent film that closes one opening surface side of the through hole in the multi-hole plate. A filler is dispersed in a rubber-like elastic body, and the film and the multi-hole plate are fixed via a transparent adhesive layer.

(2) In the multi-well plate for fluorescence measurement according to another embodiment, the adhesive layer may be on one side of the film and exposed at the bottom of the well.

(3) In the multi-well plate for fluorescence measurement according to another embodiment, the rubber-like elastic body may be silicone rubber.

(4) In the multiwell plate for fluorescence measurement according to another embodiment, the film may be a cycloolefin polymer film.

(5) In the multi-well plate for fluorescence measurement according to another embodiment, the material of the adhesive layer may be a material mainly composed of a curable silicone composition.

(6) In the multiwell plate for fluorescence measurement according to another embodiment, the light-shielding filler may be a black filler.

(7) A multi-well plate set according to an embodiment is a multi-well plate set that includes two of the above-described multi-well plates for fluorescence measurement and can have the openings of the wells facing each other.

(8) A method for producing a fluorescence measurement multiwell plate according to an embodiment is a method for producing any one of the above fluorescence measurement multiwell plates, wherein a light-shielding filler is dispersed in a rubber-like elastic body. A multi-hole plate manufacturing process for manufacturing a multi-hole plate having a plurality of through-holes corresponding to a plurality of wells along the thickness direction of the plate, and closing one opening surface side of the through-hole in the multi-hole plate A pressure-sensitive adhesive application step of applying a pressure-sensitive adhesive to one side of a transparent film, a fixing step of pressing the multi-hole plate from above the pressure-sensitive adhesive, and fixing the film and the multi-hole plate through a transparent pressure-sensitive adhesive layer; ,including.

(9) In the method for producing a fluorescence measurement multiwell plate according to another embodiment, the adhesive may be an adhesive mainly comprising a curable silicone composition.

According to the present invention, it is easy to cope with a reduction in the volume of a well, and a sample can be easily transferred from one multiwell plate to another multiwell plate.

FIG. 1 is a perspective view of a fluorescence measurement multi-well plate according to an embodiment of the present invention, an enlarged view (1A) of a part A thereof, and a line BB when cut along the line BB of the perspective view. Sectional drawing and the enlarged view (1B) of the part C are each shown. FIG. 2 shows a manufacturing method of the fluorescence measurement multiwell plate of FIG. 1 using a longitudinal sectional view and a flowchart. FIG. 3 is a longitudinal sectional view of the fluorescence measurement multiwell plate of FIG. FIG. 4 is a perspective view (4A) showing a situation where a microwell plate set including two sets of multiwell plates for fluorescence measurement of FIG. 1 is used, and before the two multiwell plates for fluorescence measurement are stacked. Sectional view (4B) of the situation where the sample D in the well of one fluorescence measurement multiwell plate is transferred into the well of the other fluorescence measurement multiwell plate by superimposing two fluorescence measurement multiwell plates Respectively.

DESCRIPTION OF SYMBOLS 1 ... Fluorescence measurement multiwell plate 2 ... Multiwell plate set 5 ... Rubber-like elastic body 6 ... Light-shielding filler 10 ... Multi-hole plate 15 ... Well 16 ... Through Hole 20 ... Film 30 ... Adhesive layer 31 ... Adhesive D ... Liquid sample (one form of sample)

Hereinafter, embodiments of the present invention will be described with reference to the drawings. The embodiments described below do not limit the invention according to the scope of claims, and all the elements and combinations thereof described in the embodiments are essential to the solution means of the present invention. Not necessarily.

1. FIG. 1 is a perspective view of a fluorescence measurement multiwell plate according to an embodiment of the present invention, an enlarged view (1A) of a part A thereof, and a section taken along line BB of the perspective view. A sectional view taken along line BB and an enlarged view (1B) of a part C are respectively shown.

A multi-well plate for fluorescence measurement (hereinafter simply referred to as “multi-well plate”) 1 according to an embodiment of the present invention includes a plurality of wells 15 for use in fluorescence measurement of a liquid sample (one form of sample). The multiwell plate 1 is preferably composed of two members. One is a multi-hole plate 10 provided with a plurality of through holes 16 corresponding to a plurality of wells 15 along the thickness direction of the plate. The other is a transparent film 20 that closes one opening surface side of the through hole 16 in the multi-hole plate 10. As shown in the enlarged view of part A, the multi-hole plate 10 is formed by dispersing the light-shielding filler 6 in the rubber-like elastic body 5. The film 20 and the multi-hole plate 10 are fixed via a transparent adhesive layer 30. The adhesive layer 30 is preferably on one side of the film 20 (the surface on the multi-hole plate 10 side) and exposed at the bottom of the well 15. Hereinafter, each detail of the multi-hole plate 10, the film 20, and the adhesion layer 30 is demonstrated.

(1) Multi-hole plate The multi-hole plate 10 is a thin plate that is substantially rectangular or substantially square in plan view. The multi-hole plate 10 is provided with a total of 1024 through-holes 16 that are 32 × 32 in length, penetrating in the thickness direction of the plate in a substantially central region in plan view. The diameter of the well 15 is not particularly limited, but for example, is preferably in the range of 0.2 to 1.0 mm, more preferably in the range of 0.3 to 0.8 mm, and even more preferably in the range of 0.4 to It can be set in the range of 0.7 mm. The length of the through hole 16 (corresponding to the thickness of the multi-hole plate 10) is not particularly limited, but is preferably in the range of 0.8 to 5.0 mm, more preferably 1.0 to 3.0 mm. In the range of 1.5 to 2.5 mm. The multi-hole plate 10 includes one protrusion 11 on each diagonal line and one through-hole 12 on each other diagonal line. When these two convex portions 11 and two through-holes 12 overlap two

identical multi-hole plates

1, 1, the convex portion 11 of one multi-hole plate 1 is replaced with the through-hole of the other multi-hole plate 1. 12 is a portion that can be fixed in the in-plane parallel direction by the method of inserting into Twelve.

The multi-hole plate 10 has a structure in which a black filler which is an example of the light-shielding filler 6 is dispersed in the rubber-like elastic body 5. The multi-hole plate 10 is a structural body mainly composed of a rubber-like elastic body 5 including a rubber-like elastic body 5 and a light-shielding filler 6 having a lower mass ratio. The reason why the light-shielding filler 6 is dispersed in the rubber-like elastic body 5 is to prevent light leakage between adjacent wells 15 and light interference accompanying therewith. Examples of the rubber-like elastic body 5 include thermosetting elastomers such as silicone rubber, urethane rubber, isoprene rubber, ethylene propylene rubber, natural rubber, ethylene propylene diene rubber, nitrile rubber (NBR), and styrene butadiene rubber (SBR); Examples thereof include thermoplastic elastomers such as urethane, ester, styrene, olefin, butadiene, and fluorine, or composites thereof. A suitable example of the rubber-like elastic body 5 is silicone rubber. The light-shielding filler 6 is not particularly limited as long as light transmission between the wells 15 can be prevented. For example, carbon (graphite, etc.), copper-chromium composite oxide, copper-chromium-zinc composite oxide, zinc sulfide Examples thereof include colored pigments typified by titanium oxide and iron oxide. The mass ratio of the light-shielding filler 6 to the rubber-like elastic body 5 is appropriately determined according to the type and size of the light-shielding filler 6 (average particle diameter, etc. for particles, fiber diameter, fiber length, etc. for fibers). It can be changed. For example, the mass ratio of the light-shielding filler 6 is preferably 0.01 to 20 parts by mass, more preferably 0.1 to 10 parts by mass, and still more preferably 0 with respect to 100 parts by mass of the rubber-like elastic body 5. 2 to 5 parts by mass. Instead of these colored pigments, an organic dye may be mixed in the rubber-like elastic body 5. The multi-hole plate 10 in this embodiment is a plate in which particulate graphite filler is dispersed in silicone rubber.

(2) Film The film 20 is a member that covers a region in which a large number of through holes 15 are gathered in the multi-hole plate 10. However, the film 20 may be large enough to cover the entire one surface of the multi-hole plate 10. The film 20 is excellent in translucency in the thickness direction. Examples of the film 20 include cycloolefin polymer (COP), polymethyl methacrylate (PMMA), silicone rubber, polycarbonate (PC), polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), and the like. More preferable examples include COP and silicone rubber. COP and silicone rubber are particularly preferable because autofluorescence, which is noise during fluorescence observation, can be kept low. Although there is no restriction | limiting in particular about the thickness of the film 20, The smaller one is desirable from the need of reducing the loss of the light quantity which reaches the bottom part of the well 15. FIG. However, it is not preferable that the hole is easily opened because the thickness is too small. From the viewpoint of such balance, the preferred range of the thickness of the film 20 is 0.1 to 1.0 mm, more preferably 0.2 to 0.8 mm, and even more preferably 0.3 to 0.00. 6 mm.

(3) Adhesive layer The adhesive layer 30 is a layer having a function of interposing between the multi-hole plate 10 and the film 20 and fixing the both 10 and 20. Similar to the film 20, the adhesive layer 30 is a layer having excellent translucency. In this embodiment, the adhesive layer 30 is exposed on one opening surface of the well 15. That is, the adhesive layer 30 exists not only between the multi-hole plate 10 and the film 20 but also at the bottom of the well 15. However, the adhesive layer 30 may not be exposed at the bottom of the well 15.

In this embodiment, the adhesive layer 30 is preferably a transparent silicone rubber layer. In that case, the material of the adhesive layer 30 is preferably a material mainly composed of a curable silicone composition. When the adhesive layer 30 is made of silicone rubber, autofluorescence is suppressed, and when the multi-hole plate 10 is made of silicone rubber, the adhesiveness with the COP or silicone rubber film 20 is excellent. Although there is no restriction | limiting in particular about the thickness of the adhesion layer 30, Since the loss of the light quantity which reaches the inside of the well 15 needs to be reduced, the thinner one is desirable. However, it is not preferable that the thickness is too small and the adhesiveness is inferior. From the viewpoint of such balance, the preferable range of the thickness of the adhesive layer 30 is 0.05 to 0.50 mm, more preferably 0.08 to 0.30 mm, and still more preferably 0.10 to 0. .25 mm.

The film 20 and the adhesive layer 30 have extremely low thickness variations in each plane. For this reason, when excitation light having the same intensity is applied, the intensity variation of light reaching the well 15 is small. That is, the thickness of the bottom of the well 15 is formed more uniformly between the wells 15 than when the well 15 that is recessed from one surface of the rubber-like elastic body 5 in which the light-shielding filler 6 is dispersed is formed. Because it can.

2. 2. Method for Manufacturing Fluorescence Measurement Multiwell Plate FIG. 2 shows a method for manufacturing the fluorescence measurement multiwell plate of FIG. 1 using a longitudinal sectional view and a flowchart.

The multiwell plate 1 can be suitably manufactured through a multi-hole plate manufacturing process (step S100), an adhesive application process (step S200), and a fixing process (step S300). Hereinafter, details of each process will be described.

(1) Multi-hole plate manufacturing process (step S100)
The multi-hole plate manufacturing step is formed by dispersing the light-shielding filler 6 in the rubber-like elastic body 5 and includes a plurality of through holes 16 corresponding to the plurality of wells 15 along the thickness direction of the plate. It is a process of producing. More specifically, a fluid obtained by kneading the light-shielding filler 6 in the curable rubber composition is supplied to a mold that can be molded into the form of the multi-hole plate 10, and the mold is clamped and heated. Perform pressure molding. A more preferable method of this molding is an injection molding method. However, you may use the press molding method which puts the plate-shaped molding after primary vulcanization in a metal mold | die, and performs secondary vulcanization. The through-hole 16 is preferably formed at the time of molding, but may be formed by mechanical means or laser processing after molding.

(2) Adhesive application process (step S200)
The pressure-sensitive adhesive application step is a step of applying the pressure-sensitive adhesive 31 to one side of the transparent film 20 that closes one opening surface side of the through hole 16 in the multi-hole plate 10. The pressure-sensitive adhesive 31 is cured to form the pressure-sensitive adhesive layer 30, and is preferably a pressure-sensitive adhesive mainly composed of a curable silicone composition. In this step, preferably, the pressure-sensitive adhesive 31 is coated on the entire surface of one side of the film 20 using a roll coater or the like. Instead of the roll coater, any type of substrate for transferring the adhesive 31 to the film 20 may be used. In that case, what is necessary is just to apply | coat the adhesive 31 to a base material and to transcribe | transfer the adhesive 31 of the said base material to the film 20. FIG. As a result, the pressure-sensitive adhesive 31 can be uniformly placed within the surface of the film 20.

(3) Fixing process (step S300)
The fixing step is a step of pressing the multi-hole plate 10 on the adhesive 31 and fixing the film 20 and the multi-hole plate 10 through the transparent adhesive layer 30. When a method of fixing the multi-hole plate 10 to a surface of the film 20 coated with the pressure-sensitive adhesive 31 mainly composed of a curable silicone composition is used, sufficient adhesive strength can be obtained only by pressure-contacting the both 10 and 20. There is a merit. Moreover, unlike the application of the adhesive, there is no need for a drying step, and further, no step for modifying the surface of the multi-hole plate 10 serving as the adherend is required. Therefore, it is possible to realize the manufacture of the multiwell plate 1 at a low cost by a simple process with few management items. In addition, even when both materials of the multi-hole plate 10 and the film 20 are different, there is an advantage that the adhesive 31 can be easily bonded by using the pressure-sensitive adhesive 31 mainly composed of the curable silicone composition.

3. Production Example 100 parts by mass of curable silicone rubber composition (Shin-Etsu Chemical Co., Ltd. product number: KE-961T-U) and black pigment (Shin-Etsu Chemical Co., Ltd. product number: KE-COLOR-BL) 0.5 Mass parts and 0.5 parts by mass of a crosslinking agent (Shin-Etsu Chemical Co., Ltd. product number: C-8A) were added, mixed uniformly, and subjected to heat and pressure molding to produce a multi-hole plate. Further, a transparent curable silicone rubber adhesive (product number: X-40-3340 manufactured by Shin-Etsu Chemical Co., Ltd.) is applied to a film made by COP (ZeonorFilm manufactured by Nippon Zeon Co., Ltd., product number: ZF16). A film with an adhesive was formed. In place of the curable silicone rubber pressure-sensitive adhesive (product number: X-40-3340 manufactured by Shin-Etsu Chemical Co., Ltd.), product number: X-40-3331-2 manufactured by the same company may be used. The curable silicone rubber adhesive was applied using a roll coater. Next, the multi-well plate after molding was pressed on the film with the adhesive to complete a multi-well plate.

4). Usage Example FIG. 3 shows a longitudinal sectional view of the fluorescence measurement multiwell plate of FIG.

When the liquid sample D is subjected to fluorescence measurement, the liquid sample D is put into each well 15 of the multi-well plate 1 and the excitation light P is incident so as to pass through the film 20 from the bottom of the well 15 containing the liquid sample D. . The excitation light P can be emitted using, for example, a laser oscillator 40. In addition, for example, when an ELISA method is applied and an antigen-antibody reaction and an enzyme-substrate reaction occur in the liquid sample D in the specific well 15, when the excitation light P is applied to the liquid sample D, the enzyme -The fluorescence of the fluorescent substance produced by the substrate reaction can be measured. The measurement object contained in the liquid sample D which is an example of the sample is not particularly limited as long as fluorescence measurement is possible. For example, peptides or proteins, or DNA or RNA encoding such information, bacteria, One or more selected from the group consisting of fungi, yeast, living cells in a single cell suspension, and hybridoma cells.

5). FIG. 4 is a perspective view of a situation in which a microwell plate set including two sets of multiwell plates for fluorescence measurement in FIG. 1 is used, and the two fluorescence measurement multiwell plates are stacked. FIG. (4A) and two fluorescence measurement multiwell plates are stacked, and the liquid sample D in the well of one fluorescence measurement multiwell plate is transferred into the well of the other fluorescence measurement multiwell plate. Sectional drawing (4B) is shown, respectively.

When two identical multi-well plates 1 are stacked, the openings of the wells 15 face each other, and the convex portions 11 of one multi-well plate 1 are inserted into the through holes 12 of the other multi-well plate 1. When the microwell plate set 2 having two such multi-shaped multiwell plates 1 is used, the liquid sample D can be transferred from one multiwell plate 1 to the other multiwell plate 1. For example, the liquid sample D placed in each well 15 of one multi-well plate 1 can be transferred to an empty well 15 of the other multi-well plate 1 and each well of one multi-well plate 1 can be transferred. It is also possible to transfer the liquid sample D placed in 15 to the well 15 containing another sample in the other multi-well plate 1 to be mixed or reacted.

The example shown in FIG. 4 (4B) is an example in which the liquid sample D placed in each well 15 of one multiwell plate 1 is transferred to an empty well 15 of the other multiwell plate 1. Two

multiwell plates

1 and 1 are overlapped so that the openings of the opposing wells 15 are aligned (a), and force is applied from one multiwell plate 1 to the other multiwell plate 1 by a method such as centrifugation. Effect. As a result, the liquid sample D put in each well 15 of one multi-well plate 1 can be transferred simultaneously to the empty wells 15 of the other multi-well plate 1.

The multi-hole plate 10 constituting the multi-well plate 1 is mainly composed of the rubber-like elastic body 5, and thus is a structural body having the flexibility of the rubber-like elastic body 5. For this reason, when the two

multi-well plates

1 and 1 are overlapped so that the openings of the wells 15 face each other, the peripheral area of the openings is likely to be in close contact. For this reason, compared with the conventional method which transfers the liquid sample D using the multiwell plate which consists of hard resin etc., the liquid sample D can be transferred easily and safely, without using an isolation sheet.

The multiwell plate set 2 has two

multiwell plates

1 and 1 as described above, and the openings of the wells 15 can face each other. The microwell plate set 2 can be used as follows, for example. A DNA having a desired promoter sequence or the like is placed in one microwell plate 1 and amplified. After the amplification is completed, a replica is prepared in the same manner as described above using the microwell plate 1 as an original. A buffer for performing in vitro translation can be put in each original well 15 to obtain a peptide as a translation product. In this original, a protein capable of binding a peptide, for example, a magnetic particle immobilized with streptavidin and a target protein immobilized on biotin is amplified, and a DNA containing a target substance and a coding sequence bound to the target substance is amplified. It can be observed by their combination. In the case of binding, for example, a fluorescent label can be further bound, the complex containing the target protein can be cleaved with an enzyme, and the supernatant can be collected to measure fluorescence.

Also, clustering and sequencing of genomic profiles can be performed by collecting the contents of the wells 15 emitting fluorescence and performing micro temperature gradient gel electrophoresis. At this time, various amplified peptides are contained in the well 15 on the receiving side. For this reason, the peptide couple | bonded with a target protein can be selected by putting the magnetic particle which couple | bonded the biotin and the streptavidin couple | bonded with the target protein in each well 15, for example.

6). Other Embodiments Although preferred embodiments of the present invention have been described above, the present invention is not limited to the above-described embodiments, and can be implemented in various modified forms.

For example, a rubber-like elastic body 5 other than silicone rubber may be used as a main material constituting the multi-hole plate 10. Furthermore, a film mainly composed of a material other than the cycloolefin polymer may be used as the film 20. Further, as the material of the adhesive layer 30, materials other than the material mainly composed of the curable silicone composition may be used.

The present invention can be used for fluorescence analysis on a sample in a well.

Claims

A multi-well plate for fluorescence measurement comprising a plurality of wells for use in fluorescence measurement of a sample,
A multi-hole plate comprising a plurality of through holes corresponding to the plurality of wells along the thickness direction of the plate;
A transparent film for closing one opening surface side of the through hole in the multi-hole plate;
With
The multi-hole plate is formed by dispersing a light-shielding filler in a rubber-like elastic body,
The multiwell plate for fluorescence measurement, wherein the film and the multi-hole plate are fixed via a transparent adhesive layer.
The multi-well plate for fluorescence measurement according to claim 1, wherein the adhesive layer is on one side of the film and exposed at the bottom of the well.
The multiwell plate for fluorescence measurement according to claim 1 or 2, wherein the rubber-like elastic body is silicone rubber.
The multi-well plate for fluorescence measurement according to any one of claims 1 to 3, wherein the film is a film of a cycloolefin polymer.
The multi-well plate for fluorescence measurement according to any one of claims 1 to 4, wherein the material of the adhesive layer is a material mainly composed of a curable silicone composition.
The multi-well plate for fluorescence measurement according to any one of claims 1 to 5, wherein the light-shielding filler is a black filler.
A multi-well plate set having two multi-well plates for fluorescence measurement according to any one of claims 1 to 6 and capable of facing each other's openings.
A method for producing a multiwell plate for fluorescence measurement according to any one of claims 1 to 6,
A multi-hole plate production step for producing a multi-hole plate comprising a plurality of through holes corresponding to a plurality of wells along the thickness direction of the light-shielding filler dispersed in a rubber-like elastic body,
An adhesive application step of applying an adhesive to one side of a transparent film that closes one opening surface side of the through hole in the multi-hole plate;
A fixing step of pressing the multi-hole plate from above the adhesive and fixing the film and the multi-hole plate through a transparent adhesive layer;
A method for producing a multiwell plate for fluorescence measurement, comprising:
The method for producing a multiwell plate for fluorescence measurement according to claim 8, wherein the pressure-sensitive adhesive is a pressure-sensitive adhesive mainly composed of a curable silicone composition.