WO2018155865A1

WO2018155865A1 - Blood analysis strip

Info

Publication number: WO2018155865A1
Application number: PCT/KR2018/001995
Authority: WO
Inventors: 김원동; 김근영
Original assignee: (주)오상헬스케어
Priority date: 2017-02-27
Filing date: 2018-02-19
Publication date: 2018-08-30
Also published as: KR20180099964A

Abstract

A blood analysis strip according to one embodiment of the present invention is a strip to be inserted into a specimen measurement device such that a specific substance contained in blood is analyzed, and comprises: a blood inlet provided so as to accommodate blood in a first state; a first channel for allowing the first state blood, having been accommodated in the blood inlet, to flow therein in a second state by a capillary phenomenon; a first chamber for collecting the second state blood, having flowed in the first channel, after the blood has passed through the first channel; a second channel for allowing the blood, having been collected in the first chamber, to flow therein in a third state by a capillary phenomenon; and a second chamber for collecting the third state blood, having flowed in the second channel, after the blood has passed through the second channel.

Description

Blood Analysis Strips

The present invention relates to a strip for blood analysis, and more specifically, glucose, ketone, albumin, glycated hemoglobin (HbA1c) in blood by optical methods and / or electromagnetic methods, etc. The present invention relates to a strip for blood analysis that can be used for analysis of hormones, minerals, lipids, proteins, vitamins, hemoglobin, and the like.

Blood circulating in the blood vessels of a human or animal body carries oxygen from the lungs to tissue cells, carries carbon dioxide from the tissues to the lungs, releases it out, transports nutrients absorbed from the digestive tract to organs or tissue cells, As a decomposition product of, it transports substances that are unnecessary to the living body to the kidneys and discharges them out of the body, carries hormones secreted from the endocrine glands to the working organs and tissues, distributes body heat evenly, maintains constant body temperature, and invades the living body It performs various functions such as destroying and detoxifying bacteria and foreign substances.

Such blood may be a sample to be analyzed for a variety of purposes, including glucose, ketones, albumin, glycated hemoglobin (HbA1c), hormones, minerals, lipids in the blood. ), Protein (vitamin), hemoglobin (hemoglobin), etc. can be analyzed to predict the abnormal signs of the body or determine the current state.

For example, the amount of glucose is a factor that can induce diabetes. If the amount of glucose is high, it causes diabetes. On the contrary, a small amount of glucose causes serious problems such as abnormal symptoms in the central nervous system.

Therefore, it is necessary to measure the amount of glucose from time to time to prevent problems due to the amount of glucose in advance, and there are various measuring means for this.

Here, the method using the light of measuring the amount of glucose is to measure the amount of glucose by irradiating light to the blood to analyze the received light.

Blood contains blood cells, such as red blood cells, and blood cells, etc., are relatively large in size compared to plasma containing glucose, and thus the amount of glucose is measured by optical methods, that is, optically, in measuring glucose. The obstacle is the reality.

Therefore, in order to measure the amount of glucose by the optical method, it is necessary to separate the obstructive blood cells from the blood plasma, and the degree of separation eventually determines the accuracy of blood glucose measurement.

However, conventionally, the method of separating plasma and blood cells from the blood is so complicated that there is a problem in terms of efficiency in measuring blood glucose by an optical method.

Eventually, glucose, ketone, albumin, glycated hemoglobin (HbA1c), hormones, minerals, lipids, proteins, vitamins, In the analysis of hemoglobin (hemoglobin) and the like, it is urgent to study to maximize the accuracy of the analysis by satisfying certain conditions.

An object of the present invention is to provide glucose, ketone, albumin, glycated hemoglobin (HbA1c), hormone (hormone), mineral (mineral), in the blood by optical methods and / or electromagnetic methods. It provides a strip inserted into a sample measuring device that can analyze lipids, proteins, vitamins, hemoglobin, and the like. By providing a separate strip for blood analysis to maximize the accuracy of the analysis.

The blood analysis strip according to an embodiment of the present invention is inserted into a sample measuring device, wherein the sample measuring device is configured to analyze a specific component contained in blood by an optical method and / or an electromagnetic method. A strip for allowing a specific component contained in the blood to be analyzed, the blood inlet being provided to receive blood in a first state; A first channel through which the blood in the first state received at the blood inlet flows into the second state by capillary action; A first chamber in which the blood of the second state introduced into the first channel is collected after passing through the first channel; A second channel for introducing blood collected in the first chamber into a third state by capillary action; And a second chamber in which the blood of the third state introduced into the second channel is collected after passing through the second channel, wherein the first channel includes blood of the first state. The blood cells are formed into a capillary tube so that the blood of the second state is filtered in the process of flowing into the second channel, and the second channel is formed by the blood cells in the process of introducing the blood collected in the first chamber into the second channel. It may be characterized in that it is formed into a capillary tube, so that the filtered blood of the third state.

In the first channel of the blood analysis strip according to an embodiment of the present invention, after the blood of the second state is introduced, the blood of the second 'state in which the blood cells are reduced from the blood of the second state is reduced. It may be characterized in that it is formed to reduce the cross-sectional area along the inflow direction of the blood to be introduced into one chamber.

In the second channel of the blood analysis strip according to an embodiment of the present invention, after the blood of the third state is introduced, the blood of the third 'state in which blood cells are reduced from the blood of the third state is reduced. It may be characterized in that the cross-sectional area along the inflow direction of the blood is reduced to be introduced into the two chambers.

The first chamber of the blood analysis strip according to an embodiment of the present invention is such that the blood of the third state is collected in the second chamber after flowing into the second channel by capillary action, the first chamber It may have a channel and a space larger than the second channel.

The second chamber of the blood analysis strip according to an embodiment of the present invention may have a larger space than the second channel to secure an analysis area of a specific component included in the blood.

Blood analysis strip according to an embodiment of the present invention, the first blood processing unit is provided in the first chamber so that the processed blood flows into the second channel by processing the blood collected in the first chamber; It may be characterized in that it further comprises.

The first blood processing unit of the blood analysis strip according to an embodiment of the present invention, the filter to pass the blood plasma in the blood collected in the first chamber, filtering the blood cells, blood cells in the blood collected in the first chamber It may be characterized in that it comprises at least one of a coagulant for coagulation and a hemolytic agent for hemolysis of red blood cells in the blood collected in the first chamber.

Blood analysis strips according to an embodiment of the present invention, the blood cells are collected in the blood collected in the second chamber to collect the specific component based on the plasma contained in the blood collected in the second chamber by the controller And a second blood processing unit provided in the second chamber to be analyzed.

Strip for blood analysis according to an embodiment of the present invention The first blood processing unit may be at least one of salt, beads, and fragments for dNTP buffer or lysis for genetic analysis.

The second chamber of the blood analysis strip according to an embodiment of the present invention may be exposed to the outside.

According to the blood analysis strip according to the present invention, blood cells and plasma in the blood are separated from each other by an effective and simple method, such as glucose, ketone, and blood in the blood by an optical method and an electromagnetic method. It is possible to maximize the accuracy of the analysis of albumin, glycated hemoglobin (HbA1c), hormones, minerals, lipids, proteins, vitamins, hemoglobin, etc. .

BRIEF DESCRIPTION OF THE DRAWINGS The figure for demonstrating the principle by which the specific component of blood is analyzed by the sample measuring apparatus.

2 and 3 are schematic perspective and exploded perspective views showing a strip for blood analysis according to a first embodiment of the present invention.

Figure 4 is a schematic exploded perspective view showing a strip for blood analysis according to a second embodiment of the present invention.

5 is a schematic exploded perspective view showing a strip for blood analysis according to a third embodiment of the present invention.

Figure 6 is a schematic exploded perspective view showing a strip for blood analysis according to a fourth embodiment of the present invention.

7 is a schematic exploded perspective view showing a strip for blood analysis according to a fifth embodiment of the present invention.

Hereinafter, with reference to the drawings will be described in detail a specific embodiment of the present invention. However, the spirit of the present invention is not limited to the embodiments presented, and those skilled in the art who understand the spirit of the present invention may deteriorate other inventions or the present invention by adding, modifying, or deleting other elements within the scope of the same idea. Other embodiments that fall within the scope of the inventive concept may be readily proposed, but they will also be included within the scope of the inventive concept.

In addition, the components with the same functions within the scope of the same idea shown in the drawings of each embodiment will be described using the same reference numerals.

1 is a view for explaining the principle that a specific component of blood is analyzed by a sample measuring device.

Referring to Figure 1, the blood analysis strip (100, hereinafter referred to as 'strip') according to the present invention, such as glucose (keco), ketone (keton), in the blood by a spectroscopic method and / or an electromagnetic method, etc. In a sample measuring device that can analyze albumin (albumin), glycated hemoglobin (HbA1c), hormone (hormone), mineral (mineral), lipid (lipid), protein (protein), vitamin (vitamin), hemoglobin It may be a strip to be inserted.

In addition, the strip 100 may be a strip used for gene amplification for gene analysis, nucleic acid extraction for nucleic acid analysis, and various other purposes.

The blood analysis strip 100 may be capable of stimulating thermal, physical, optical, and electrical (electrophoresis, electrophoresis, etc.) in the sample measuring device, and the sample measuring device may include the blood analysis strip 100. A correction algorithm may be applied to correct blood phase or characteristics of processes, for example, external environmental factors such as temperature or humidity.

The sample measuring device may be a device capable of analyzing a specific component in blood by an optical method and / or an electromagnetic method, but is not limited thereto, and may be specified by an electrochemical method or a physical invasion method. It may be a device capable of analyzing the components.

Hereinafter, the case by the optical method will be described as an example.

The optical method may mean a method capable of analyzing the concentration of the specific component or the like based on the property of the light irradiated to the blood by the specific component included in the blood.

Here, the strip 100 according to the present invention is glucose (kecose), ketone (keton), albumin (albumin), glycated hemoglobin (HbA1c), hormone (hormone), mineral ( It may be a strip that separates blood cells and plasma so that specific components such as minerals, lipids, proteins, vitamins, hemoglobin, etc. can be analyzed, but below the concentration of glucose The case where is measured will be described by way of example, and the purpose of the strip 100 according to the present invention is not limited to the purpose for measuring the concentration of glucose.

In order to measure the concentration of glucose by an optical method, light may be irradiated to the plasma, and the light irradiated to the plasma containing glucose used in the optical method may be infrared light, that is, a wavelength range of 800-2,500 nm. It may be near infrared (NIR) or mid infrared (MIR) in the wavelength range of 2,500-10,000 nm.

Herein, the light may be reflected or transmitted with scattering by the glucose contained in the plasma. Here, the concentration of glucose may be measured based on light reflected with scattering, and scattering may be performed. It is also possible to measure the concentration of glucose based on the light transmitted while accompanying.

That is, the optical method can analyze a specific component in the blood in either a reflection type or a transmission type.

When measuring the concentration of glucose based on the reflected light with the scattering, spectroscopy the received light to obtain a spectrum, and based on the acquired spectrum, a dynamic spectrum representing a change in the spectrum over time is obtained. The concentration of glucose may be measured by comparing the generated dynamic spectrum with previously stored concentration-specific dynamic spectrum.

And, when measuring the concentration of glucose based on the light transmitted with the scattering, the concentration of glucose can be measured based on the amount of change in the intensity of light received based on the intensity of light irradiated to the plasma.

In other words, the degree of scattering of light is determined based on the number of particles of glucose in the plasma. That is, the more particles, the less scattering of light. When the scattering of light is reduced, the distance that light passes through the plasma becomes shorter. Decreases the rate of decrease.

This is due to the fact that the glucose contained in the plasma decreases the refractive index between the plasma. Consequently, the higher the concentration of glucose, the less the ratio of the lowering of the light intensity. Can be.

In order to measure the concentration of glucose by the optical method as described above, the specimen measuring device includes a light emitting part (X) for irradiating the plasma containing glucose and a light receiving part for receiving the light irradiated from the light emitting part (X). (Y1, Y2), a control unit (not shown) for calculating the concentration of glucose based on the light received by the light receiving unit (Y1, Y2).

Here, the light receiving units Y1 and Y2 measure the concentration of glucose based on the light receiving unit Y1 for measuring glucose concentration and / or the light transmitted while scattering, based on the light reflected with scattering. The light receiving unit Y2 may be included.

On the other hand, light and analysis data that can be used in analyzing a specific component contained in the blood in the optical method is not limited to the above-mentioned, it may use a monochromatic light or multiple wavelengths of various spectra, absorbance, luminance, Analysis may also be made through reflectance, spectrum, colorimetry, and / or color pattern.

2 and 3 are a schematic perspective view and a schematic exploded perspective view showing a strip for blood analysis according to a first embodiment of the present invention.

2 and 3, the strip 100 according to the first embodiment of the present invention is a strip inserted into a sample measuring apparatus for measuring the concentration of glucose described with reference to FIG. 1. It may be a strip that allows plasma to be separated from each other by an effective and simple method to maximize the accuracy of the measurement of glucose concentration.

Here, blood contains blood cells, such as red blood cells, and the like, because blood cells, etc., are relatively large in size compared to the plasma containing glucose, thereby impeding the measurement of blood glucose by spectroscopic method, thereby increasing the concentration of glucose. Accurate measurement requires the separation of blood cells and plasma.

For this reason, the strip 100 according to an embodiment of the present invention is the blood inlet 121, the first channel 122, the first chamber 123, the second channel 124, the second chamber 125 and Third channel 126 and the like.

The blood inlet 121 may be a component provided to receive the blood B1 in a first state including plasma and blood cells, and is formed such that the cross-sectional area along the moving direction of the blood is reduced linearly or nonlinearly. Can be.

The first channel 122 may be a channel for introducing the blood B1 of the first state received in the blood inlet 121 into the second state by a capillary phenomenon.

The first chamber 123 may be a chamber in which the blood B2 of the second state introduced into the first channel 122 passes through the first channel 122 and is collected. The channel 124 may be a channel for introducing blood collected in the first chamber 123 into a third state by capillary action.

The second chamber 125 may be a chamber in which the blood B3 of the third state introduced into the second channel 124 passes after passing through the second channel 124. Channel 126 is an optional component and may be a channel communicating with the outside to improve capillary phenomenon.

Here, the blood B1 of the first state, the blood B2 of the second state, and the blood B3 of the third state are terms defined to distinguish blood according to the amount of blood cells contained in the blood. .

The blood inlet 121, the first channel 122, the first chamber 123, the second channel 124, the second chamber 125 and the third channel 126 are predetermined spaces. The intermediate layer 120 having a predetermined size, the upper layer 110 and the lower layer 130 of a predetermined size bonded to the upper and lower sides of the intermediate layer 120, respectively.

The intermediate layer 120, the upper layer 110 and the lower layer 130 may be formed of a material that does not absorb blood, the upper layer 110 and the lower layer 130 is a material that can transmit light, For example, it may be formed of a transparent material.

The blood inlet 121, the first channel 122, the first chamber 123, the second channel 124, the second chamber 125 and the third channel 126 may be formed in the intermediate layer ( The size may be determined by a predetermined space of 120, but is not necessarily limited thereto.

That is, in addition to a predetermined space formed in the intermediate layer 120, a space is formed in at least one of the upper layer 110 and the lower layer 130, and the components may be implemented by the sum of the spaces.

In addition, the third channel 126 may be implemented by a space formed in at least one of the upper layer 110 and the lower layer 130 to prevent separation of the intermediate layer.

Hereinafter, the blood inlet 121, the first channel 122, the first chamber 123, the second channel 124, the second chamber 125 and the third channel 126 are The case where the size is determined by a predetermined space of the intermediate layer 120 will be described as an example.

To form the blood inlet 121, the first channel 122, the first chamber 123, the second channel 124, the second chamber 125 and the third channel 126. The predetermined space formed in the intermediate layer 120 may be covered by an upper side and a lower side by the upper layer 110 and the lower layer 130.

The first channel 122 may be formed as a capillary tube so that the blood B2 of the second state, from which blood cells are filtered, is introduced while the blood B1 of the first state flows into the first channel 122. In the second channel 124, the blood B3 of the third state in which the blood cells are filtered is introduced while the blood collected in the first chamber 123 flows into the second channel 124. It may be formed as a capillary tube.

Since the first channel 122 is formed as a capillary, relatively large blood cells are primarily filtered in the blood B1 in the first state accommodated in the blood inlet 121 to reduce the amount of blood cells. Blood B2 in two states is introduced into the first channel 122.

In addition, since the second channel 124 is formed as a capillary tube, blood cells having a relatively large size in the blood collected in the first chamber 123 are secondarily filtered to reduce the amount of blood cells. (B3) is introduced into the second channel 124, and finally the blood is minimized the amount of blood cells are collected in the second chamber (125).

When the blood having the minimum amount of blood cells is collected in the second chamber 125, the light emitting unit X of the sample measuring device irradiates light to the blood in the second chamber 125, and the light receiving unit Y1, After Y2) receives the light, a specific component contained in blood in the second chamber 125 is analyzed by the controller. However, the specimen measuring device may irradiate light to blood in the first chamber 123 to analyze specific components (eg, hemoglobin concentration, etc.) of blood in the first chamber 123 based on the light. .

In addition, the blood in the blood of the first chamber 123 and the blood of the second chamber 125 may be irradiated simultaneously or sequentially so that different specific components of blood may be analyzed based on the light.

On the other hand, the first chamber 123 is the blood (B3) of the third state is introduced into the second channel 124 by the capillary phenomenon, and then collected in the second chamber 125, the first A space larger than the channel 122 and / or the second channel 124 may be provided.

This is because the first channel 122 and the middle in order to maximize the movement force of the blood by the capillary phenomenon that is the source of the force that can move the blood from the first channel 122 to the second channel 124 A larger space than the second channel 124 is required, and the first chamber 123 is required for this purpose.

In addition, when the specific component (eg, hemoglobin concentration, etc.) of the blood in the first chamber 123 is analyzed by the sample measuring device due to the spatial characteristics as described above of the first chamber 123, the sample measuring device The irradiation area of the light emitted from the light emitting portion X can be increased.

The first chamber 123 may have a shape that becomes wider and narrower along a moving direction of blood, and may be, for example, an ellipse, but is not limited thereto.

In addition, the second chamber 125 may have a larger space than the second channel 124 in order to increase the irradiation area of the light emitted by the light emitting unit X of the specimen measuring device.

As a result, glucose concentration measurement by spectroscopic method targeting blood including plasma collected in the second chamber 125 may be more accurate.

Like the first chamber 123, the second chamber 125 may have a shape that is widened and narrowed along a moving direction of blood, and may be, for example, an ellipse, but is not limited thereto.

The first channel 122 and the second channel 124 may have a constant cross-sectional area in a blood moving direction, but may be different in cross-sectional area according to a moving direction of blood as described below.

On the other hand, the third channel 126 may be a channel communicating with the outside to improve the capillary phenomenon, the blood from the blood inlet 121 by the capillary phenomenon without the third channel 126 from the second chamber If so, the third channel 126 can be omitted.

Basically, because capillary phenomena are caused by intermolecular adhesion, ie, intermolecular adhesion of blood, they can also occur in closed channels without the third channel 126.

In this case, however, the direction of gravity and the direction of atmospheric pressure are different.

On the other hand, the above has been described a case in which the blood analysis strip according to the present invention includes two chambers, two or three channels, but is not necessarily limited thereto, and the number of chambers or the number of channels is changed as necessary. It can be revealed.

4 is a schematic exploded perspective view showing a strip for blood analysis according to a second embodiment of the present invention.

Referring to FIG. 4, the strip 200 according to the second embodiment of the present invention may include a first channel 222 and the second channel 224, and the first channel 222 and the first channel 222. At least one of the two channels 224 may be formed such that the cross-sectional area along the direction of blood movement is reduced (in FIG. 4, both the first channel 222 and the second channel 224 are along the direction of blood movement). Is shown to be reduced).

Specifically, after the blood B2 in the second state is introduced into the first channel 222, the blood in the second 'state in which the blood cells are reduced from the blood B2 in the second state is reduced in the first chamber 223. ), The cross-sectional area along the inflow direction of blood may be reduced.

In addition, after the blood B3 of the third state is introduced into the second channel 224, the blood of the third 'state in which blood cells are reduced from the blood B3 of the third state is reduced in the second chamber 225. ), The cross-sectional area along the inflow direction of blood may be reduced.

As described above, the amount of blood cells contained in the blood can be naturally filtered while passing through the first channel 222 and the second channel 224, so that the effect of blood cell removal becomes more remarkable.

5 is a schematic exploded perspective view showing a strip for blood analysis according to a third embodiment of the present invention, and FIG. 6 is a schematic exploded perspective view showing a strip for blood analysis according to a fourth embodiment of the present invention.

First, referring to FIG. 5, the upper layer 310 may include an upper exposure part 315 formed through the second chamber 325 to be exposed to the outside.

Plasma collected in the second chamber 325 due to the upper exposure portion 315 may be exposed to the outside.

This is because when the upper layer 310 is not formed of a transparent material through which light is transmitted, it is possible to measure the concentration of glucose based on spectroscopic method, that is, light reflected while being scattered.

Referring to FIG. 6, each of the upper layer 410 and the lower layer 430 may include an upper exposed portion 415 and a lower exposed portion 435 formed through the second chamber 425 so as to be exposed to the outside. .

This is because when the upper layer 410 and the lower layer 430 are not formed of a transparent material through which light is transmitted, it is possible to measure the concentration of glucose based on spectroscopic method, that is, based on the transmitted light with scattering. to be.

Referring to FIG. 7, the strip 500 according to the fifth embodiment of the present invention allows a specific process to be performed based on the blood collected in the first chamber 523 so that the blood after the specific process is performed is It may include a first blood processing unit 550 to be introduced into the second channel 524.

For example, when the strip 500 according to the present invention is a strip for measuring the concentration of glucose in the blood as described above, the first blood processing unit 550 is connected to the first chamber 523. It may be a component provided in the first chamber 523 to reduce the blood cell amount of the blood (B3) of the third state flowing into the second channel 524 by collecting blood cells in the collected blood. .

In this case, the first blood processing unit 550 may be a kind of filter that allows blood to pass through the plasma, but filters the blood cells of a relatively large size, and the second channel 524 in the first chamber 523. Any position may be provided as long as it does not block the entrance.

This is to prevent a decrease in blood movement due to a bottleneck during blood movement, and the first blood processing part 550 may be a filter formed of paper, glass fiber, nanofiber, porous layer medium, polymer separation membrane, or the like. It may be formed of one or several layers of multilayers, but is not necessarily limited thereto, and any one having a characteristic of filtering blood cells and passing only plasma may be applicable.

* Here, when the first blood processing unit 550 is implemented with the above-described filter, it may be possible to control whether or not filtering or the degree of filtering, internally or externally.

As described above, the first blood processing unit 550 is not limited to a filter, but a coagulant that coagulates blood cells in the blood collected in the first chamber 523 and / or hemolyzes the red blood cells in the blood. Eg hemoglobin, lymphocytes, etc.) may be a hemolytic agent to be analyzed.

When the first blood processing unit 550 includes a coagulant, the coagulant may be silica particles, citric acid, or the like, but is not necessarily limited thereto, and any coagulant may be used as long as it can coagulate blood cells.

When the first blood processing unit 550 includes a hemolytic agent, the hemolytic agent may be Lysercell or the like. In this case, the sample measuring device may be applied to the blood hemolyzed by the hemolytic agent in the first chamber 123. The light may be irradiated with respect to a specific component (eg, hemoglobin concentration, etc.) of blood in the first chamber 123 based on the light.

Here, the first blood processing unit 550 may be various reagents or enzymes in addition to the coagulant and / or hemolytic agent.

The coagulant, hemolytic agent, various reagents and / or enzymes may be provided in at least one of the first channel and the second channel.

The first blood processing unit 550 does not necessarily have to be provided with the above-described filter, a coagulant and / or a hemolytic agent independently, and the filter and a coagulant and / or a hemolytic agent are simultaneously provided in the first chamber 523. On the other hand, although not shown, the second chamber 525 collects blood cells in the blood collected in the second chamber 525 so that the second chamber 525 is controlled by the control unit of the sample measuring device. A second blood processing unit may be provided to allow a specific component to be analyzed based on the plasma contained in the blood collected in the).

The second blood processing unit may have the same configuration as the first blood processing unit 550, but preferably implemented as a filter.

Here, when the second blood processing unit is implemented as a filter, it may be possible to control whether the filtering or the degree of filtering, internally or externally.

Meanwhile, when the strip 500 according to the present invention is a strip for gene amplification for gene analysis, the first blood processing unit 550 may be a dNTPs buffer and may further include a porous membrane for physical filtering. .

The strip 500 may include a third chamber in addition to the first chamber 523 and the second chamber 525, and the third channel 126 may include the second chamber 525 and the third chamber. It can function as a connection channel for connecting.

In addition, the strip 500 may include a fourth channel, which is a channel communicating with the outside of the third chamber to improve a capillary phenomenon.

In this case, the second blood processing part which may be provided in the second chamber 525 may be a polymerize which is a kind of enzyme, and thus, the blood flowing into the second chamber 525 may be the second blood processing part. After mixing with the polymerize, it is introduced into the third chamber through the third channel 126 and combined with the primer provided in the third chamber.

Thereafter, denaturation, annealing and polymerization processes are repeated to finally achieve gene amplification.

In addition, when the strip 500 according to the present invention is a strip used for nucleic acid extraction for nucleic acid analysis, the first blood processing unit 550 may be a salt, a bead, a treatment, or the like. The second blood processing unit may be a washing solution for cleaning.

In the third chamber, the column to which the hydroxyl group is attached may be chemically filtered so that only the nucleic acid that needs to be analyzed may remain, and the remaining proteins or lipids may be separated and then the beads may be illuminated.

Here, a known material for chemical filtering may be present as the second blood processing part in the third chamber, and the third chamber may be implemented in plural.

Meanwhile, the first blood processing unit 550 may be formed of a lysercell or surfactant, and nanofibers to separate blood cells, and the second blood processing unit may be implemented with various reactive enzymes to form specific substances in the blood. Can be analyzed.

In this case, the measurement of a specific substance may proceed through an external response stimulus.

In the above description of the configuration and features of the present invention based on the embodiment according to the present invention, the present invention is not limited thereto, and various changes or modifications can be made within the spirit and scope of the present invention. It will be apparent to those skilled in the art that such changes or modifications fall within the scope of the appended claims.

For example, in the present invention, the size, number, direction of travel of the first channel, the second channel, and the third channel may be variously changed within the spirit and scope of the present invention. The size, number, shape, etc. of the first chamber and the second chamber may also be variously changed within the spirit and scope of the present invention.

That is, the present invention may be arranged in a plane or at least one of at least one of the first channel, the second channel, the third channel, the first chamber, and the second chamber.

In addition, the present invention may form another channel and another chamber in addition to the first channel, the second channel, the third channel, the first chamber and the second chamber according to the type of the specific component. have.

Claims

In the blood analysis strip which is inserted into a sample measuring device for analyzing a specific component contained in the blood so that the specific component contained in the blood is analyzed,

A blood inlet provided for receiving blood in a first state;

A first channel through which the blood in the first state received at the blood inlet flows into the second state by capillary action;

A first chamber in which the blood of the second state introduced into the first channel is collected after passing through the first channel;

A second channel for introducing blood collected in the first chamber into a third state by capillary action; And

And a second chamber in which the blood of the third state introduced into the second channel is collected after passing through the second channel.

The first channel,

When the blood of the first state is introduced into the first channel, the blood cells are formed into a capillary tube, so that the blood of the second state filtered out,

The second channel,

The blood analysis strip of claim 1, wherein the blood collected in the first chamber flows into the second channel.
The method of claim 1,

The first channel,

After the blood of the second state is introduced, the cross-sectional area along the inflow direction of the blood is reduced so that the blood of the second 'state in which blood cells are reduced from the blood of the second state is introduced into the first chamber. Characterized by the blood analysis strip.
The method of claim 1,

The second channel,

After the blood of the third state is introduced, the cross-sectional area along the inflow direction of the blood is reduced so that the blood of the third 'state in which blood cells are reduced from the blood of the third state is introduced into the second chamber. Characterized by the blood analysis strip.
The method of claim 1,

The first chamber,

After the blood of the third state is introduced into the second channel by the capillary phenomenon, the first channel and a larger space than the second channel so as to be collected in the second chamber, characterized in that for blood analysis strip.
The method of claim 1,

The second chamber,

And a space larger than the second channel to secure an analysis region of a specific component included in the blood.
The method of claim 1,

A first blood processing unit provided in the first chamber to process the blood collected in the first chamber and the processed blood flows into the second channel; Blood analysis strips further comprising.
The method of claim 6,

The first blood processing unit,

Among the filters that pass the plasma in the blood collected in the first chamber but filters the blood cells, a coagulant that coagulates the blood cells in the blood collected in the first chamber and a hemolytic agent that hemolyses red blood cells in the blood collected in the first chamber. A blood analysis strip comprising at least one.
The method of claim 1,

A second blood processing unit provided in the second chamber so that the blood cells are collected in the blood collected in the second chamber so that the specific component is analyzed based on the plasma contained in the blood collected in the second chamber by the controller. Blood analysis strips, characterized in that it further comprises.
The method of claim 6,

The first blood processing unit,

At least one of a salt, beads, or a piece for the dNTP buffer or lysis for genetic analysis.
The method of claim 1,

The second chamber,

Strip for blood analysis, characterized in that exposed to the outside.