WO2018152422A1 - Dosage à surface texturée - Google Patents

Dosage à surface texturée Download PDF

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Publication number
WO2018152422A1
WO2018152422A1 PCT/US2018/018521 US2018018521W WO2018152422A1 WO 2018152422 A1 WO2018152422 A1 WO 2018152422A1 US 2018018521 W US2018018521 W US 2018018521W WO 2018152422 A1 WO2018152422 A1 WO 2018152422A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
plates
plate
spacers
prior embodiments
Prior art date
Application number
PCT/US2018/018521
Other languages
English (en)
Inventor
Stephen Y. Chou
Ji QI
Wei Ding
Yufan ZHANG
Original Assignee
Essenlix Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2018/017307 external-priority patent/WO2018148342A1/fr
Priority claimed from PCT/US2018/017501 external-priority patent/WO2018148469A1/fr
Priority claimed from PCT/US2018/017504 external-priority patent/WO2018148471A2/fr
Priority claimed from PCT/US2018/017492 external-priority patent/WO2018148461A1/fr
Priority claimed from PCT/US2018/017494 external-priority patent/WO2018148463A1/fr
Priority claimed from PCT/US2018/017502 external-priority patent/WO2018148470A1/fr
Priority claimed from PCT/US2018/017489 external-priority patent/WO2018148458A1/fr
Priority claimed from PCT/US2018/017712 external-priority patent/WO2018148606A1/fr
Priority claimed from PCT/US2018/017713 external-priority patent/WO2018148607A1/fr
Priority claimed from PCT/US2018/017716 external-priority patent/WO2018148609A2/fr
Priority claimed from PCT/US2018/018007 external-priority patent/WO2018148729A1/fr
Priority claimed from PCT/US2018/018108 external-priority patent/WO2018148764A1/fr
Priority claimed from PCT/US2018/017499 external-priority patent/WO2018152005A1/fr
Priority claimed from PCT/US2018/018405 external-priority patent/WO2018152351A1/fr
Priority to JP2019544634A priority Critical patent/JP7107953B2/ja
Priority to CN201880025156.1A priority patent/CN111448449A/zh
Priority to CA3053301A priority patent/CA3053301A1/fr
Priority to US16/485,347 priority patent/US10966634B2/en
Application filed by Essenlix Corporation filed Critical Essenlix Corporation
Publication of WO2018152422A1 publication Critical patent/WO2018152422A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food

Definitions

  • PCT/US18/17716 filed on 2/9/2018 (18F08), PCT Application No. PCT/US18/17713, filed on 2/9/2018 (18F07), PCT Application No. PCT/US18/17712, filed on 2/9/2018 (18F16), PCT Application No. PCT/US18/17504, filed on 2/8/2018 (18F02), PCT Application No.
  • PCT/US18/17494 filed on 2/8/2018 (18F02), PCT Application No. PCT/US18/17502, filed on 2/8/2018 (18F09), and PCT Application No. PCT/US18/17307, filed on 2/7/2018 (18F15A), each of which is incorporated herein by reference in its entirety for all purposes.
  • the present invention is related to devices and methods of performing biological and chemical assays, devices and methods of performing a biological and chemical using colorimetric approaches.
  • the present invention provides solutions to the to improve the sensitivity, speed, and easy-to-use of assaying by optical signal, such as colorimetric assays or fluorescent assays.
  • Fig. 1-A illustrates an example of opened assembled colorimetric assay sample card comprising a bottom plate, a top plate and an aluminum hinge, in accordance with an embodiment of the present invention.
  • Fig. 1-B and Fig. 1 -C illustrate an example of bottom plate of the colorimetric assay sample card having textured microstructures on top surface, in accordance with an embodiment of the present invention.
  • Fig. 1-D and Fig. 1-E illustrate an example of top plate of the colorimetric assay sample card having pillar arrays of uniform heights on bottom surface, in accordance with an embodiment of the present invention.
  • FIG.1 -F and FIG. 1-G illustrates an example of ready-to-test colorimetric assay sample card comprising a bottom plate, a top plate, an aluminum hinge and sample liquid between top and bottom plates, in accordance with an embodiment of the present invention.
  • FIG. 2-A illustrates a test apparatus of colorimetric measurement of sample with textured surfaces using side illumination of a fiber.
  • Fig. 2-B, Fig. 2-C and Fig. 2-D illustrates a test apparatus of colorimetric measurement of sample with textured surfaces using ring illumination of a fiber.
  • Fig. 3 is an illustration of a CROF (Compressed Regulated Open Flow) embodiment.
  • Panel (a) illustrates a first plate and a second plate wherein the first plate has spacers.
  • Panel (b) illustrates depositing a sample on the first plate (shown), or the second plate (not shown), or both (not shown) at an open configuration.
  • Panel (c) illustrates (i) u sing the two plates to spread the sample (the sample flow between the plates) and reduce the sample thickness, and (ii) using the spacers and the plate to regulate the sample thickness at the closed configuration.
  • the inner surface of each plate may have one or a plurality of binding sites and or storage sites (not shown).
  • Fig. 4 A diagram of a process of testing heavy metal in water. DETAILED DESCRIPTION OF EXMAPLARY EMBODIMENTS
  • an assay involving a detection of light signal such as colorimetric assay or fluoresceuse assay
  • a small container to hold a liquid sample and passes a light beam though the sample to measure the light or the color of the sample.
  • the sample is very thin, the light or color becomes faint and difficult measure.
  • the present invention provides, among other thing, solution to get a stronger optical signal in a thin sample.
  • One novelty of the present invention is to use QMAX card (that has two movable plates) to make a sample into a very uniform thin layer (less than 200 urn).
  • Another novelty of the present invention is to use a textured reflective surface on a surface of one of the two plates to enhance an optical signal, particularly for colorimetric assay and/or fluorescence assay.
  • the color signal of a colorimetric assay can be significantly increased by using a reflective textured surface as one of the wall of the chamber can significantly increase the color signal.
  • a device uses to plates to sandwich a sample into a thin layer, wherein one of the plate is transparent and the other plate has a textured reflective surface on its sample contact area.
  • the probing light enters the sample from the transparent plate, goes through the sample, and diffusively reflected by the textured surface back to the transparent plate.
  • the device further comprise a dry reagent coated on one of the plate, so that a liquid sample can dropped on one or both of the plate, close the plates, and then measurement.
  • the sample thickness can be 150 urn or less, making the dry regent mixed with the sample in a short time, to speed up the total measurement time.
  • CROF Card or card
  • COF Card or card
  • COF Card QMAX-Card
  • Q-Card CROF device
  • COF device COF device
  • QMAX-device CROF plates
  • COF plates COF plates
  • QMAX-plates are interchangeable, except that in some embodiments, the COF card does not comprise spacers; and the terms refer to a device that comprises a first plate and a second plate that are movable relative to each other into different configurations (including an open configuration and a closed configuration), and that comprises spacers (except some embodiments of the COF) that regulate the spacing between the plates.
  • X-plate refers to one of the two plates in a CROF card, wherein the spacers are fixed to this plate. More descriptions of the COF Card, CROF Card, and X-plate are described in in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on
  • a device for assaying a sample using optical signal comprising:
  • first and second plates are movable relative to each other into different configurations
  • the second plate has, on its inner surface, have textured structures for
  • the textured surface can be, but is not limited to a bumpy, wavy roughly
  • the textured surface can be periodic or aperiodic; vi. the textured surface's average roughness range is preferred to be, but is not limited to 2um ⁇ 5um;
  • the spacers are fixed to the inner surface of the first plate and have a
  • the preferred height of spacers is larger than the average roughness of the textured surface and smaller than 100um;
  • a closed configuration which is configured after the sample deposition in the open configuration, and in the closed configuration: at least part of the deposited sample is compressed by the two plates into a continuous layer;
  • Device_C1 for colorimetric signal
  • a sample handling device for enhancing optical signal comprising:
  • a first plate, a second plate, spacers and textured surface wherein:
  • the plates are movable relative to each other into different configurations; ii. one or both plates are flexible;
  • the second plate has, on its inner surface, have textured structures for
  • the textured surface can be, but is not limited to a bumpy, wavy roughly surface
  • the textured surface can be periodic or aperiodic
  • the textured surface's average roughness range is preferred to be, but is not limited to 2um ⁇ 5um;
  • the spacers are fixed to the inner surface of the first plate and have a
  • the preferred height of spacers is larger than the average roughness of the textured surface and smaller than 100um;
  • a closed configuration which is configured after the sample deposition in the open configuration, and in the closed configuration: at least part of the deposited sample is compressed by the two plates into a continuous layer;
  • the sample is in liquid form.
  • the textured surface is made of opaque white material
  • a sample handling device for enhancing optical signal comprising:
  • a first plate, a second plate, spacers and textured surface wherein:
  • the plates are movable relative to each other into different configurations; ii. one or both plates are flexible;
  • the second plate has, on its inner surface, have textured structures for
  • the textured surface can be, but is not limited to a bumpy, wavy roughly surface
  • the textured surface can be periodic or aperiodic
  • the textured surface's average roughness range is preferred to be, but is not limited to 2um ⁇ 5um;
  • the spacers are fixed to the inner surface of the first plate and have a
  • the preferred height of spacers is larger than the average roughness of the textured surface and smaller than 100um;
  • a closed configuration which is configured after the sample deposition in the open configuration, and in the closed configuration: at least part of the deposited sample is compressed by the two plates into a continuous layer;
  • the textured surface is made of semi-opaque white material, and the transmissivity is 10% -30%;
  • a sample handling device for enhancing optical signal comprising:
  • a first plate, a second plate, spacers and textured surface wherein:
  • the plates are movable relative to each other into different configurations; ii. one or both plates are flexible;
  • the second plate has, on its inner surface, have textured structures for
  • the textured surface can be, but is not limited to a bumpy, wavy roughly surface; v. the textured surface can be periodic or aperiodic;
  • the textured surface's average roughness range is preferred to be, but is not limited to 2um ⁇ 5um;
  • the spacers are fixed to the inner surface of the first plate and have a
  • the preferred height of spacers is larger than the average roughness of the textured surface and smaller than 100um;
  • a closed configuration which is configured after the sample deposition in the open configuration, and in the closed configuration: at least part of the deposited sample is compressed by the two plates into a continuous layer;
  • the textured surface is made of opaque white material or coated with reflective metal film
  • the metal film can be, but is not limited to aluminum, silver and gold.
  • the preferred thickness range of the metal film is preferred to be, but not limited to be 10nm ⁇ 100nm. Apparatus (for colorimetric signal).A1
  • a testing apparatus comprising:
  • a mobile computing device having a camera module and a light source; c) an Illumination optics, comprising a tilted optical fiber;
  • the light source emits white light
  • the preferred distance between them is 15mm ⁇ 20mm;
  • the external lens is put between the Q-card and camera module so that the sample in Q-card is in the working distance of camera module, and the preferred focal length of external lens is 12-18mm, and the distance between lens and camera module is preferred to be as small as possible and no larger than 3mm;
  • the optical fiber guide the light emitted from the light source to illuminate on the sample area right under the camera module; -wherein one end face of the optical fiber is put under the aperture of the light source, and the distance between them is preferred to be as small as possible and no larger than 3mm; -wherein the diameter of the optical fiber is configured to be equal to the diameter of the light source aperture;
  • the tilt angle in which the optical fiber is mounted is set to make the center light beam emitted out from the fiber illuminate on the sample area right under the camera module.
  • a testing apparatus comprising:
  • a mobile computing device having a camera module and a light source
  • an Illumination optics comprising a pair of reflective mirrors
  • the light source emits white light
  • the external lens is put between the Q-card and camera module so that the sample in Q-card is in the working distance of camera module, and the preferred focal length of external lens is 4 ⁇ 8mm, and the preferred distance between lens and camera module is preferred to be as small as possible and no larger than 3mm;
  • the illumination optics turns the light emitted from the light source to back-illuminate the sample on Q-card, and each mirror turns the light by 90 degree;
  • the mirrors are mounted under the Q-card, and one mirror is in a line with the light source, and another one is in a line with the camera module, and the preferred distance between the Q-card and mirrors is 5mm ⁇ 10mm.
  • a testing apparatus comprising:
  • an external lens -wherein the light source emits white light, and the light source is put under the Q-card and in a line with the camera module, and the preferred distance between the light source and Q- card is 5mm ⁇ 10mm.
  • the external lens is put between the Q-card and camera module so that the sample in Q-card is in the working distance of camera module, and the preferred focal length of external lens is 4 ⁇ 8mm, and the preferred distance between lens and camera module is preferred to be as small as possible and no larger than 3mm;
  • the optical signal that can by enhanced by the textured surfaces of the device of any prior embodiment is selected from a group of colors in the sample, fluorescence, luminescence (electrical, chemical, photo, or electrical-chemical), and/or other light from emitters.
  • a testing apparatus comprising:
  • an Illumination optics comprising tilted reflective mirror
  • filters comprising a long pass filter and a short pass filter
  • the light source is a laser diode
  • the tilt mirror turns the light emitted from the light source to illuminate on the sample area right under the camera module
  • the preferred distance between them is 15mm ⁇ 20mm;
  • the external lens is put between the Q-card and camera module so that the sample in Q-card is in the working distance of camera module, and the preferred focal length of external lens is 12mm ⁇ 18mm, and the preferred distance between lens and camera module is preferred to be as small as possible and no larger than 3mm;
  • a method for analyzing the optical signal of sample comprising the steps of:
  • the mobile computing device process the image to analyze colorimetric or fluorescent signal of the image to get some property of the sample.
  • the Q-card device, testing apparatus and the method above can be applied to detect presence and level of the analyte of interest in the following fields:
  • Food science and safety testing pH, ammonia, nitrite, nitrate, heavy metal, bacteria level, etc. in drinking water; testing bacteria, lactose, additive, particular protein level, etc. in milk;
  • a device for enhancing an optical signal in assaying comprises:
  • the first and second plates are movable relative to each other into different configurations, and have, on its respective inner surface, a sample contact area for contacting a sample that contains an analyte;
  • one or both of the plates are flexible
  • the first plate is transparent to the light
  • the second plate substantially reflect light and comprises an inner surface a light scattering layer that has a rough topology
  • one of the configurations is an open configuration, in which the average spacing between the inner surfaces of the two plates is at least 200 um, and the sample is deposited on one or both of the plates; wherein another of the configurations is a close configuration, which is configured after the sample deposition in the open configuration, and in the closed configuration: at least part of the sample is between the two plates and the average spacing between the inner surfaces of the plates is less than 200 urn; and
  • the light scattering layer enhances trapping a probe light between the inner surface of the two plates.
  • the light scattering surface of the second plate comprises:
  • the textured surface can be, but is not limited to a bumpy, wavy roughly
  • the textured surface can be periodic or aperiodic
  • the textured surface's average roughness range is preferred to be, but is not limited to 2um ⁇ 5um; or
  • the spacers are fixed to the inner surface of the first plate and have a
  • the light scattering layer can be made of highly reflectively opaque white material with reflectivity at least 50%, 60%, 70%, 80%, 90%, 100%, or in a range between any of the two values.
  • the light scattering layer can be made of semi-opaque white material, and the transmissivity is 10% -30%.
  • the light scattering layer can be made of reflective metal film, wherein the light scattering layer can be made of opaque white dielectric film.
  • the textured surface can be periodic or aperiodic, wherein the shape of a single feature on the textured surface can be but not limited to square, triangle, sharp corner.
  • the average roughness height (R a ) of the textured reflective need to be at least 20% of the wavelength of the illumination light and can be up to 5-fold of the spacing between the first plate and second plate, or in range between these two values;
  • (b a ) need to be at least 20% and up to 10-fold of the wavelength of the illumination light, or in range between these two values;
  • the average period (b a ) need to be at least 50% and up to 1000-fold of the wavelength of the illumination light, or in range between these two value.
  • FIG. 1 -A is the schematic illustration of colorimetric assay sample card 1 in open status.
  • Sample card 1 comprises a top plate 12, a bottom plate 11 and an aluminum hinge 13.
  • Hinge 13 attach top plate 12 to bottom plate 13.
  • the height of the random scattering structures is from 1 nm to 200 nm, from 1 nm to
  • the reflection surface can be done by random nanoparticles of the same size or different size.
  • the reflective range from 50 % to 100%, from 30% to 100 % and from 50% to 80%. They are either wide band or narrow band in spectrum,
  • FIG. 1-B and FIG. 1 -C are the schematic illustrations of bottom plate 11 in sample card 1 , shown from isometric view and cross-section view respectively.
  • the material for the bottom plate 11 is nonabsorbent and has opaque white color. It can be, but is not limited to, white polyethylene.
  • the bottom plate 11 has textured surface 11 S on one of its top surface (i.e. the surface facing the top plate 12).
  • the textured surface 11 S can be random microstructures or periodic microstructures. For random microstructures, it can be, but is not limited to, a bumpy, wavy or rough surface. In one embodiment, the textured surface is the bumpy surface of the matte finish of the white polystyrene sheet with average roughness of 2-3 um.
  • notch 11 N is fabricated on one side of bottom plate 11 to make it easy to open top plate 12.
  • a triangle gap 11 C is fabricated at one corner of bottom plate 11 to easily differentiate the front and bottom surface of bottom plate 11.
  • FIG. 1-D and FIG. 1-E are the schematic illustrations of top plate 12 in sample card 1 , shown from isometric view and cross-section view respectively.
  • the material for the top plate is transparent and can be, but is not limited to, PMMA.
  • On bottom surface of the top plate i.e. the surface facing the bottom plate 11), there are periodic micro-size pillar arrays 12S with uniform heights.
  • the pillar array can be, but is not limited to, rectangular pillars with square lattice.
  • the top plate is made of PMMA of 175um thickness and the pillar array has a square lattice with the period of 120um*110um.
  • each pillar has the rectangular shape with the dimension of 30um*40um and the pillar height is 30um.
  • a triangle gap 12C is fabricated at one corner of bottom plate 12 to easily differentiate the front and bottom surfaces of top plate 12.
  • FIG. 1-F and FIG. 1-G are the schematic illustrations of colorimetric assay sample card 1 in closed status with sample liquid, shown from isometric view and cross-section view respectively.
  • the sample liquid 1 L is embedded between top plate 12 and bottom plate 11.
  • the textured surface 11S of bottom plate 11 is towards the bottom surface of the top plate 12 with pillar array 12S.
  • the average liquid layer thickness of the sample liquid 1 L is uniform and determined by height of the pillar array 12S on top plate 12. Hence, the volume of the sample liquid 1 L holding in sample card 1 per unit area in this present invention can be accurately determined.
  • textured surface 11 S of bottom plate 11 helps deflect the light beams to increase the light path inside the sample liquid layer 1 L. Hence, light absorption by the colored compounds in sample liquid 1 L is increased and the color change is enhanced.
  • FIG. 2-A, 2-B and 2-C are the schematic views showing details of system 10 reading a colorimetric card, and particularly of device 13.
  • FIG. 15-A is the sectional view showing details of device 13.
  • FIG. 15-B and FIG. 15-C are the schematic views only showing the configuration of the optics elements in device 13. These figures illustrate the functionality of the elements that were described above with reference to FIG. 14.
  • the light emitted from light source 1 L is coupled into side-emitting optical fiber ring 135 from the two end faces of fiber ring 135 and travels inside along the ring.
  • Beam B1 is emitted out from the side wall of fiber ring and go through the diffuser film 136.
  • Beam B1 illuminates the sample area of colorimetric sample card 138 right under the camera 1C from front side to create uniform illumination.
  • the illuminated sample area absorbs part of beam B1 and reflects the beam B1 to beam B2.
  • Beam B2 is collected by lens 133 and gets into camera 1C
  • Lens 133 creates an image of the sample area on the image sensor plane of camera 1C.
  • Smartphone 1 captures and processes the image to analyze the color information in the image to quantify the color change of the colorimetric assay.
  • no spacers are used in regulating the sample thickness between the two plates.
  • the textured reflective surface of the plate has one or a combination of each of the parameters:
  • Colorimetric assay's signal can be enhanced by the textured surfaces.
  • colorimetric assay under the illumination of white light, a specific wavelength of light is absorbed by the colored compounds, which results in the color change. Hence, to get stronger color change signal, more light of the specific absorbing wavelength of the color compounds needs to get absorbed.
  • Beer-Lambert law which determines how much percent of light is absorbed when light passing through a light absorbing medium, the way to increase the light absorption in a colorimetric assay is to increase the light path in the sample liquid.
  • the textured surface can make the small-angle incident light be reflected to a large- angle emergent light by scattering to increase the light path in the sample liquid.
  • textured surface can scatter the incident light several times in the sample liquid to increase the light path before the light emits out.
  • Fluorescent signal of an assay can also be enhanced by the textured surface.
  • the emitting fluorescent intensity is proportional to the product of fluorescent dye's quantum yield and absorbed amount of excitation light.
  • the textured surface increases the light path of excitation light in the sample liquid by scattering hence more excitation light is absorbed by the fluorescent molucules.
  • a test apparatus comprises the device, a light source, an optical fiber and an imager -wherein the light source emits light within wavelength range of 300nm to 1000nm; -wherein the light source and imager are on a same plane; -wherein the Q-card is put right under the imager, the preferred distance between them is 15mm ⁇ 20mm;
  • optical fiber guide the light emitted from the light source to illuminate on the sample area right under the camera module
  • one end face of the optical fiber is put under the aperture of the light source, and the distance between them is preferred to be as small as possible and no larger than 10mm;
  • the diameter of the optical fiber is configured to be equal to the diameter of the light source aperture
  • the tilt angle in which the optical fiber is mounted is set to make the center light beam emitted out from the fiber illuminate on the sample area right under the camera module.
  • a test apparatus comprises the device, a light source, a ring-shape optical fiber and an image,
  • the light source emits light within wavelength range of 300nm to 1000nm; wherein the ring fiber is a side-emitting optical fiber that can outcouple light from the wall of the fiber;
  • the preferred distance between them is 15mm ⁇ 20mm;
  • a light diffuser is put between the ring-shape fiber and sample to diffuse the light emitting from the ring fiber;
  • a QMAX card uses two plates to manipulate the shape of a sample into a thin layer (e.g. by compressing) (as illustrated in Fig. 1).
  • the plate manipulation needs to change the relative position (termed: plate configuration) of the two plates several times by human hands or other external forces. There is a need to design the QMAX card to make the hand operation easy and fast.
  • one of the plate configurations is an open configuration, wherein the two plates are completely or partially separated (the spacing between the plates is not controlled by spacers) and a sample can be deposited.
  • Another configuration is a closed configuration, wherein at least part of the sample deposited in the open configuration is compressed by the two plates into a layer of highly uniform thickness, the uniform thickness of the layer is confined by the inner surfaces of the plates and is regulated by the plates and the spacers.
  • the reagents e.g. detection agent and binding agent
  • some reagents e.g. detection agent
  • the detection agent it is desirable for the detection agent to be added after the substantial binding of the target analyte by the binding agent.
  • the present invention is to provide devices and methods for achieving these goals as well as for making bio/chemical sensing (including, not limited to, immunoassay, nucleic assay, electrolyte analysis, etc.) faster, more sensitive, less steps, easy to perform, smaller amount of samples required, less or reduced (or no) needs for professional assistance, and/or lower cost, than many current sensing methods and devices.
  • bio/chemical sensing including, not limited to, immunoassay, nucleic assay, electrolyte analysis, etc.
  • compressed open flow refers to a method that changes the shape of a flowable sample deposited on a plate by (i) placing other plate on top of at least a part of the sample and (ii) then compressing the sample between the two plates by pushing the two plates towards each other; wherein the compression reduces a thickness of at least a part of the sample and makes the sample flow into open spaces between the plates.
  • CROF compressed regulated open flow
  • SCCOF self-calibrated compressed open flow
  • QMAX QMAX
  • spacers or “stoppers” refers to, unless stated otherwise, the mechanical objects that set, when being placed between two plates, a limit on the minimum spacing between the two plates that can be reached when compressing the two plates together. Namely, in the compressing, the spacers will stop the relative movement of the two plates to prevent the plate spacing becoming less than a preset (i.e. predetermined) value.
  • a spacer has a predetermined height
  • a spacer is fixed on its respective plate in a QMAX process means that the spacer is attached to a location of a plate and the attachment to that location is maintained during a QMAX (i.e. the location of the spacer on respective plate does not change) process.
  • An example of "a spacer is fixed with its respective plate” is that a spacer is monolithically made of one piece of material of the plate, and the location of the spacer relative to the plate surface does not change during the QMAX process.
  • a spacer is not fixed with its respective plate
  • a spacer is glued to a plate by an adhesive, but during a use of the plate, during the QMAX process, the adhesive cannot hold the spacer at its original location on the plate surface and the spacer moves away from its original location on the plate surface.
  • open configuration of the two plates in a QMAX process means a configuration in which the two plates are either partially or completely separated apart and the spacing between the plates is not regulated by the spacers
  • the term "closed configuration" of the two plates in a QMAX process means a configuration in which the plates are facing each other, the spacers and a relevant volume of the sample are between the plates, the relevant spacing between the plates, and thus the thickness of the relevant volume of the sample, is regulated by the plates and the spacers, wherein the relevant volume is at least a portion of an entire volume of the sample.
  • a sample thickness is regulated by the plate and the spacers in a QMAX process means that for a give condition of the plates, the sample, the spacer, and the plate compressing method, the thickness of at least a port of the sample at the closed configuration of the plates can be predetermined from the properties of the spacers and the plate.
  • inner surface or “sample surface” of a plate in a QMAX device refers to the surface of the plate that touches the sample, while the other surface (that does not touch the sample) of the plate is termed “outer surface”.
  • spacer height is the dimension of the spacer in the direction normal to a surface of the plate, and the spacer height and the spacer thickness means the same thing.
  • area of an object in a QMAX process refers to, unless specifically stated, the area of the object that is parallel to a surface of the plate.
  • spacer area is the area of the spacer that is parallel to a surface of the plate.
  • QMAX device refers the device that perform a QMAX (e.g. CROF) process on a sample, and have or not have a hinge that connect the two plates.
  • QMAX e.g. CROF
  • QMAX cards do not use spacers to control the sample thickness in a closed configuration of the movable plates, rather they use other ways to measure the sample thickness after reaching a closed configuration.
  • the thickness measurements include light interference measurements.
  • colorimetric and grammatical variants thereof refer to the physical description and quantification of the color spectrum including the human color perception spectrum (e.g., visible spectrum).
  • a colorimetric assay is particularly useful when quantification is not necessary and where expensive detection equipment is unavailable.
  • detection of the color change can be carried out by naked eye observation of a user (e.g., the person performing the assay). Because a colorimetric assay can be detected by naked eye observation, a user can either examine the reaction for a detectable change in color or the assay can be carried out in parallel with one or more controls (positive or negative) that replicate the color of a comparable reaction. In some embodiments, calibrated colorimetric measurements could be used to determine the amount of target quantitatively.
  • a colorimetric analysis involves determining the presence/absence, level, or concentration of an analyte (such as a chemical element or chemical compound) in a sample, such as a solution, with the aid of a color reagent. It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic reaction step.
  • the equipment required is a colorimeter, one or more cuvettes, and a suitable color reagent.
  • the process may be automated, e.g., by the use of an AutoAnalyzer or by Flow injection analysis.
  • colorimeters can be adapted for use with plate readers to speed up analysis and reduce the waste stream.
  • a colorimetric assay disclosed herein is a non-enzymatic method.
  • a metal ion can react with one or more agents to form one or more colored products.
  • calcium can react with o-cresolphthalein complexone to form a colored complex; copper may react with bathocuproin disulfonate to form a colored complex; creatinine can react with picrate to form a colored complex; iron can react with bathophenanthroline disulfonate to form a colored complex; and phosphate can react with ammonium molybdate and/or ammonium metavanadate to form a colored complex.
  • a colorimetric assay disclosed herein comprises one or more enzymatic reaction step.
  • the color reaction is preceded by a reaction catalyzed by an enzyme.
  • the enzyme is specific to one or more particular substrates, more accurate results can be obtained. For example, in an assay for cholesterol detection such as the CHOD-PAP method), cholesterol in a sample is first reacted with oxygen, catalyzed by the enzyme cholesterol oxidase), to produce cholestenone and hydrogen peroxide. The hydrogen peroxide is then reacted with 4-aminophenazone and phenol, this round catalyzed by a peroxidase, to produce a colored complex and water.
  • Another example is the GOD-Perid method for detecting glucose, where glucose is a sample is first reacted with oxygen and water, catalyzed by the enzyme glucose oxidase, to generate gluconate and hydrogen peroxide.
  • the hydrogen peroxide so generated then reacts with ABTS to produce a colored complex, and the reaction can be catalyzed by a peroxidase.
  • the so-called GPO-PAP method detects triglycerides, which are first converted to glycerol and carboxylic acid (catalyzed by an esterase); the glycerol is then reacted with ATP to form glycerol-3-phosphate and ADP (catalyzed by a glycerol kinase); the glycerol-3-phosphate is then oxidized by a glycerol-3-phosphate oxidase to form dihydroxyacetone phosphate and hydrogen peroxide; and the final enzymatic reaction is catalyzed by a peroxidase, where the hydrogen peroxide reacts with 4-aminophenazone and 4-chlorophenol to form a colored complex.
  • the colorimetric assay may comprise both non-enzymatic step(s) and enzymatic step(s).
  • urea can be detected by first converting the analyte into ammonium carbonate (catalyzed by a urease), and then the ammonium carbonate reacts with phenol and hypochlorite in a non-enzymatic reaction to form a colored complex.
  • a colorimetric assay detects a protein target.
  • a colorimetric assay involves the formation of a protein-metal chelation (such as protein-copper chelation), followed by secondary detection of the reduced metal (e.g., copper). Examples of this type of colorimetric assay include the BCA assay and the Lowry protein assay, such as the Thermo Scientific Pierce BCA and Modified Lowry Protein Assays.
  • a colorimetric assay involves protein-dye binding with direct detection of the color change associated with the bound dye. Examples of this type of colorimetric assay include the 660 nm assay and the Coomassie (Bradford) protein assay.
  • colorimetric assays for detecting a polypeptide or protein target include the Biuret assay, the Bicinchoninic Acid (Smith) assay, the Amido Black method, and the Colloidal Gold assay.
  • the colorimetric assay such as a colorimetric screening, can be based on NAD(P)H generation.
  • the absorbance of NAD(P)H at 340 nm is commonly used to measure the activity of dehydrogenases.
  • this type of colorimetric assay involves an indirect method requiring either a synthetic compound or a secondary enzyme.
  • tetrazolium salts such as nitroblue tetrazolium (NBT) can be reduced to formazan dyes, which absorb light in the visible region.
  • NBT nitroblue tetrazolium
  • a cascade reaction leading to the formation of a colored formazan links the production of NAD(P)H to the catalytic activity of a dehydrogenase in a sample.
  • the colorimetric assay is an Enzyme-Linked Immunosorbent Assay (ELISA).
  • ELISA substrates include colorimetric (also called chromogenic) substrate for alkaline phosphatase (AP) and/or horseradish peroxidase enzyme (HRP), such as PNPP (p-Nitrophenyl Phosphate, a widely used substrate for detecting alkaline phosphatase in ELISA applications to produce a yellow water-soluble reaction product that absorbs light at 405 nm), ABTS (2,2'-Azinobis [3- ethylbenzothiazoline-6-sulfonic acid]-diammonium salt, which is used to detect HRP and yields a water-soluble green end reaction product), OPD (o-phenylenediamine dihydrochloride, which is used to detect HRP and yields a water soluble yellow-orange reaction product), and TMB (3,3',5,5'-te
  • PNPP p
  • a colorimetric assay examples include the HRP/ABTS/H202 Assay, HRP/4CN/H202 Assay, the D-Amino Acid Oxidase Assay, the Peroxidase/o-Dianisidine Assay, the ABTS and o-Dianisidine Assay, the TMB Assay, the Guaiacol Assay, the MNBDH Assay, assays based on the Gibbs' Reagent and 4-Aminoantipyrine, the Poly R-478 Assay, the Horseradish Peroxidase-coupled Assay, the MTT assay, the Indole Assay.and the para- Nitrophenoxy Analog (pNA) Assay.
  • HRP/ABTS/H202 Assay examples include the HRP/4CN/H202 Assay, the D-Amino Acid Oxidase Assay, the Peroxidase/o-Dianisidine Assay, the ABTS and o-Dianisidine Assay, the TMB Assay, the Guaiacol
  • Suitable colorimetric assays include, but are not limited to, colorimetric assays that detect proteins, nucleic acids, antibodies, or microorganisms. Colorimetric assays may be used to determine the concentration of a substance in a solution. In some cases, the colorimetric assays include colorimetric immunoassays.
  • Suitable colorimetric assays may include those described in Jiang et al., Analyst (2016), 141 : 1 196-1208; Morbioli et al., Anal. Chim. Acta. (2017), 970: 1-22; Gu et al., Biotechnology /Advances (2015), 33: 666-690; Marin et al., Analyst. ⁇ 2Mb), 140(1): 59-70; Du et al., Small. (2013), 9 (9-10): 1467-81 ; Song et al., Adv. Mater. (2011), 23 (37):4215-36; Liu et al., Nanoscale (2011), 3(4): 1421-33; Martin et al. J. Animicrob Chemother. (2007) 59 (2): 175- 83; Sapan et al. Biotechnol. Appl. Biochem. (1999), 29(pt 2): 99-108.
  • Colorimetric immunoassays can include enzyme immunoassays such as, e.g., an enzyme-linked immunosorbent assay (ELISA).
  • ELISA assays can include labeling a surface bound antigen with an enzyme, e.g., with a single antibody conjugate or two or more antibodies working in concert to label the antigen with the enzyme.
  • An antigen may be immobilized on a solid surface by non-specific means (e.g., adsorption) or by specific means (e.g., capture by an antibody, in a "sandwich” ELISA). The incubation can be followed by washing steps and the addition of a detection antibody covalently linked to an enzyme.
  • the detection antibody is a primary antibody that is itself detected by a secondary antibody linked to an enzyme.
  • the enzyme is reacted with an appropriate substrate, such as a chromogenic substrate, in such a manner as to produce a signal, e.g., a chemical signal, that may be detected, e.g., by spectrophotometric, fluorimetric or by visual means.
  • a signal e.g., a chemical signal
  • Such color change may indicate the presence and/or quantity of the antigen in the sample.
  • Types of ELISA assays include, for example, direct ELISA, sandwich ELISA, and competitive ELISA.
  • Suitable enzymes for use in enzyme immunoassays include, but are not limited to, malate dehydrogenase, staphylococcal nuclease, delta-5-steroid isomerase, yeast alcohol dehydrogenase, alpha-glycerophosphate, dehydrogenase, triose phosphate isomerase, horseradish peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta- galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase and acetylcholinesterase.
  • the detection in such assays can be accomplished by colorimetric methods which employ a chromogenic substrate for the enzyme, where suitable substrates include, but are not limited to: o-phenylenediamine (OPD), 3, 3', 5,5'- tetramethylbenzidine (TMB), 3,3'-diaminobenzide tetrahydrochloride (DAB) , 2,2'-azino- bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the like.
  • a fluid composition of the substrate e.g., an aqueous preparation of the substrate, is typically incubated with the substrate surface for a period of time sufficient for the detectable product to be produced.
  • Incubation typically lasts for a period of time ranging from about 10 sec to 2 hours, usually from about 30 sec to 1 hour and more usually from about 5 min to 15 min at a temperature ranging from about 0 to 37°C, usually from about 15 to 30°C and more usually from about 18 to 25°C.
  • Colorimetric immunoassays can include lateral flow assays (LFA) or
  • immunochromatography assays Such assays may be performed on a series of capillary beds, e.g., porous paper or polymers, for transporting fluid.
  • Conventional lateral flow test strips include a solid support on which a sample receiving area and the target capture zones are supported.
  • the solid support material is one which is capable of supporting the sample receiving area and target capture zones and providing for the capillary flow of sample out from the sample receiving area to the target capture zones when the lateral flow test strip is exposed to an appropriate solvent or buffer, which acts as a carrier liquid for the sample.
  • Specific classes of materials which may be used as supports include organic or inorganic polymers, and natural and synthetic polymers. More specific examples of suitable solid supports include, without limitation, glass fiber, cellulose, nylon, crosslinked dextran, various chromatographic papers and nitrocellulose.
  • capture molecules may bind the complex, producing a color change in the test strip.
  • the capture zones may include one or more components of a signal producing system.
  • the signal producing system may vary widely depending on the particular nature of the lateral flow assay and may be any directly or indirectly detectable label.
  • Suitable detectable labels for use in the LFA include any moiety that is detectable by spectroscopic, photochemical, biochemical, immunochemical, electrical, optical, chemical, or other means.
  • suitable labels include biotin for staining with labeled streptavidin conjugate, fluorescent dyes (e.g., fluorescein, Texas red, rhodamine, green fluorescent protein, and the like), radiolabels (e.g.
  • enzymes e.g., horseradish peroxidase, alkaline phosphatase and others commonly used in an ELISA
  • colorimetric labels such as colloidal gold nanoparticles, silver nanoparticles, magnetic nanoparticles, cerium oxide nanoparticles, carbon nanotubes, graphene oxide, conjugated polymers, or colored glass or plastic (e.g., polystyrene, polypropylene, latex beads).
  • Radiolabels can be detected using photographic film or scintillation counters, fluorescent markers can be detected using a photodetector to detect emitted light.
  • Enzymatic labels are typically detected by providing the enzyme with a substrate and detecting the reaction product produced by the action of the enzyme on the substrate, and colorimetric labels are detected by simply visualizing the colored label.
  • the colorimetric assay may be used to measure ions in a sample.
  • chloride ions can be measured by a colorimetric assay.
  • Chloride ions displace thiocyanate from mercuric thiocyanate. Free thiocyanate reacts with ferric ions to form a colored complex - ferric thiocyanate, which is measured photometrically.
  • magnesium can be measured colorimetrically using calmagite, which turns a red-violet color upon reaction with magnesium; by a formazan dye test; emits at 600nm upon reaction with magnesium or using methylthymol blue, which binds with magnesium to form a blue colored complex.
  • calcium can be detected by a colorimetric technique using O- Cresolphtalein, which turns a violet color upon reaction of O-Cresolphtalein complexone with calcium.
  • bicarbonate can be tested bichromatically because bicarbonate (HC03-) and phosphoenolpyruvate (PEP) are converted to oxaloacetate and phosphate in the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC).
  • PEPC phosphoenolpyruvate carboxylase
  • MD Malate dehydrogenase
  • NADH reduced nicotinamide adenine dinucleotide
  • Blood urea nitrogen can be detected in a colorimetric test in which diacetyl, or fearon develops a yellow chromogen with urea and can be quantified by photometry.
  • creatinine can be measured colorimetrically, by treated the sample with alkaline picrate solution to yield a red complex.
  • creatine can be measured using a non-Jaffe reaction that measures ammonia generated when creatinine is hydrolyzed by creatinine iminohydrolase.
  • Glucose can be measured in an assay in which blood is exposed to a fixed quantity of glucose oxidase for a finite period of time to estimate concentration. After the specified time, excess blood is removed and the color is allowed to develop, which is used to estimate glucose concentration.
  • glucose oxidase reaction with glucose forms nascent oxygen, which converts potassium iodide (in the filter paper) to iodine, forming a brown color.
  • concentration of glycosylated hemoglobin as an indirect read of the level of glucose in the blood.
  • Plasma high-density lipoprotein cholesterol (HDL-C) determination is measured by the same procedures used for plasma total cholesterol, after precipitation of apoprotein B- containing lipoproteins in whole plasma (LDL and VLDL) by heparin-manganese chloride.
  • LDL and VLDL whole plasma
  • These compounds can also be detected colorimetrically in an assay that is based on the enzyme driven reaction that quantifies both cholesterol esters and free cholesterol.
  • Cholesterol esters are hydrolyzed via cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase into the ketone cholest-4-en-3-one plus hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific colorimetric probe.
  • Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1 : 1 ratio. Samples may be compared to a known concentration of cholesterol standard.
  • Reagent Recipe 1 100 units/ml Glucose Oxidase, 100 unit/ml Horseradish Peroxidase, 20mM 4-amino antipyrine, 20mM TOOS
  • Reagent Recipe 1 530 mg/ml CNPG3, 0.36 unit/ml a-Amylase, 250 mg/ml Calcium acetate
  • Reagent Recipe 1 100 unit/ml Cholesterol Oxidase, 100 unit/ml Horseradish Peroxidase, 20mM 4-amino antipyrine, 20mM TOOS
  • PI Propidium Iodide
  • FITC Fluorescein Isothiocyanate
  • B021 Basic Orange 21
  • PI Propidium Iodide
  • FITC Fluorescein Isothiocyanate
  • B021 Basic Orange 21
  • One aspect of the present invention provides systems and methods of analyzing bio/chemical sample using QMAX device.
  • a method for analyzing a sample comprising:
  • the Q-card comprises two plates that are movable relative to each other and have an open configuration and a closed configuration
  • the sample is deposited on one or both plates of the Q-Card at the open configuration, and at the closed configuration at least a part of the sample is between the two plates,
  • the mobile communication device is configured to produce an image of the Q card in the adaptor and transmit the image and/or an analysis result of the same to a remote location.
  • AA4 The method of any prior embodiment, wherein the anomaly is identified if the analysis results produced by the remote device and the mobile handheld communication device differ by a pre-defined value.
  • the sample comprises a body fluid selected from the group consisting of: amniotic fluid, aqueous humour, vitreous humour, blood (e.g., whole blood, fractionated blood, plasma, serum, etc.), breast milk, cerebrospinal fluid (CSF), cerumen (earwax), chyle, chime, endolymph, perilymph, feces, gastric acid, gastric juice, lymph, mucus (including nasal drainage and phlegm), pericardial fluid, peritoneal fluid, pleural fluid, pus, rheum, saliva, sebum (skin oil), semen, sputum, sweat, synovial fluid, tears, vomit, urine and exhaled condensate.
  • a body fluid selected from the group consisting of: amniotic fluid, aqueous humour, vitreous humour, blood (e.g., whole blood, fractionated blood, plasma, serum, etc.), breast milk, cerebrospinal fluid
  • the sample comprises a foodstuff specimen that includes: raw food ingredients, cooked or processed food, plant and animal sources of food, preprocessed food, or fully processed food.
  • the Q-card is pressed by human hand.
  • step e) comprises comparing the result to a threshold or normal range to identify samples that contain an anomaly.
  • AA10 The method of any prior embodiment, wherein the method further comprises updating the handheld mobile communication device if the analysis at the remote location produces a result that is significantly different. AA11. The method of any prior embodiment, wherein the sample deposited onto the Q-card is from a subject, and the analysis result is not transmitted to the subject.
  • AA17 The method of embodiment AA16, wherein the follow-up information comprises an explanation of the result, education about a disease or condition, information related to a possible treatment, information on the location of a suitable physician, information related to change of diet and/or exercises, or an advertisement.
  • the Q-card comprises spacers that have a substantially uniform height and a predetermined constant inter spacer distance, and in the closed configuration: at least part of the sample is compressed by the two plates of the Q-card into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the inner surfaces of the two plates and is regulated by the plates and the spacers.
  • AA20 The method of embodiment AA19, wherein for the flexible plate, the thickness of the flexible plate times the Young's modulus of the flexible plate is in the range 60 to 750 GPa- um. AA21.
  • spacers regulating the layer of uniform thickness have a filling factor of at least 1 %, wherein the filling factor is the ratio of the spacer area in contact with the layer of uniform thickness to the total plate area in contact with the layer of uniform thickness.
  • AA25 The method of any prior embodiment, wherein one or both plates comprises a scale marker, either on a surface of or inside the plate, that provide information of a lateral dimension of a structure of the sample and/or the plate.
  • AA26 The method of any prior embodiment, wherein one or both plates comprises an imaging marker, either on surface of or inside the plate, that assists an imaging of the sample.
  • AA27 The method of embodiment AA18, wherein the spacers functions as a location marker, a scale marker, an imaging marker, or any combination of thereof.
  • each spacer has the ratio of the lateral dimension of the spacer to its height is at least 1.
  • AA36 The method of embodiment AA18, wherein the minimum lateral dimension of spacer is less than or substantially equal to the minimum dimension of an analyte in the sample.
  • AA37 The method of embodiment AA18, wherein the minimum lateral dimension of spacer is in the range of 0.5 um to 100 um.
  • AA42 The method of embodiment AA18, wherein, for a pressure that compresses the plates, the spacers are not compressible and/or, independently, only one of the plates is flexible. AA43. The method of any prior embodiment, wherein the flexible plate has a thickness in the range of 10 um to 200 um.
  • a system for analyzing a sample comprising:
  • a Q-card for manipulating a sample for analysis comprising two plates that are
  • a handheld mobile communication device that comprises a camera
  • an adaptor having a slot that is configured to hold a closed Q-Card, wherein the adaptor connects to the handheld mobile communication device and permits the camera to take an image of closed Q-Card;
  • a remote device that is capable of storing information and communicating with the mobile communication device
  • the sample is deposited on one or both plates of the Q-Card at the open configuration, and at the closed configuration at least a part of the sample is between the two plates,
  • system is configured to produce an image of the Q card in the adaptor and transmit the image and/or an analysis result of the same to a remote location.
  • a method for providing healthcare recommendations to a subject comprising:
  • healthcare recommendations comprise suggestions related to medicine, nutrition/diet, exercise, and/or treatment for the subject.
  • Another aspect of the present invention provides devices and methods of cholesterol testing using QMAX device.
  • a method of analyzing a liquid sample comprising:
  • each plate respectively comprises an inner surface that has a
  • the spacers have a predetermined substantially uniform height, and at least one of the spacers is inside the sample contact area;
  • sample contact surfaces comprise one or more storage sites that store one or more reagents, which are configured to dissolve and diffuse in the sample in the closed configuration, and react with cholesterol in the sample to produce or alter a luminescence signal;
  • reading step (e) comprises detecting and quantifying the colormetric luminescence signal from the analyte in the layer of highly uniform thickness.
  • BA5 The method of paragraph BA4, wherein the color probe comprises 4- aminophenazone and phenol.
  • BA6 The method of paragraph BA1 , wherein the one or more storage sites comprise a first storage site located on the first plate and a second storage site located on the second plate.
  • the first storage site comprises cholesteryl ester hydrolase and cholesterol oxidase
  • the second storage site comprises 4-aminophenazone, phenol and
  • Heavy metal testing Another aspect of the present invention provides devices and methods of heavy metal testing in bio/chemical samples. More specifically, the invention provides a process for detecting heavy metal ions in an aqueous system, a device comprising the heavy metal ion test piece and a sensor. A portable test method provided by the device according to the invention, so as to detect the heavy metal ions in a convenient, efficient and rapid manner.
  • the heavy metal (ion) pollution refers to the environmental pollution caused by heavy metals or their compounds.
  • the increase of the heavy metal content in the environment, especially in the case of heavy metal pollution in an aqueous system, is mainly due to human factors, such as mining, waste gas emission, sewage irrigation and the use of heavy metal-contaning products, which results in the deterioration of environmental quality.
  • a heavy metal ion test piece which can be used to detect the small amount, even trace amount of heavy metal ions in an aqueous system in a simple, low cost, highly sensitive, highly reliable and stable manner. Meanwhile, it is required that the test piece is available for in situ detection, and is capable of detecting heavy metal ions with high sensitivity. Moreover, it is desired that the heavy metal ions can be not only qualitatively detected, but also quantitatively or semi-quantitatively detected. The current invention provides devices and methods for achieving these goals.
  • Fig. C1 shows that the invention comprises two parts: 1. Test, which comprises a test card that has dried reagent in a volume-controlled sample chamber, and can be inserted into a smartphone-based reader for measurement; 2. Calculation, which comprises a method to convert the photograph taken by smartphone and convert to signal for calculating analyte concentrations.
  • Test which comprises a test card that has dried reagent in a volume-controlled sample chamber, and can be inserted into a smartphone-based reader for measurement
  • Calculation which comprises a method to convert the photograph taken by smartphone and convert to signal for calculating analyte concentrations.
  • this invention is a device and method for obtaining a point-of-collection, selected quantitative indicia of an analyte on a test platform, comprising:
  • a first plate which is a coerce white substrate, is printed uniformly with color indicator as well as pH regulating agent.
  • the color indicator is bio/chemical reagent that shows specific reaction to heavy metals in liquid sample.
  • the liquid sample includes, but is not limited to, water, soil sample, oil, body fluid and food.
  • the sample is drinking water.
  • the sample is food.
  • the first plate is a coerce white polystyrene plate.
  • the color indicator is dried on the first plate.
  • the pH regulating agent is dried on the first plate.
  • the concentration of dried color indicator is 1 uM to 10 mM.
  • the concentration of dried pH regulating agent is 1 uM to 10 mM.
  • the surface of the first plate facing the second plate is defined as the inner surface of the first plate; the surface of the second plate that faces the first plate are also defined as the inner surface of the second plate.
  • the inner surfaces of the respective plates comprise a sample contact area for contacting a sample that comprises an analyte.
  • the sample contact area can occupy part or the entirety of the respective inner surface.
  • a pH regulating agent for testing heavy metal in water using colorimetric tests, a pH regulating agent must add to the sample to adjust the pH level to optimum condition. This is because the chemical reaction rate of color indicator to heavy metal ions changes significantly at different pH level, which leads to large color variation within tests if the pH is unregulated.
  • a pH regulating agent for heavy metal test, a pH regulating agent, or a combination of multiple combination of them, is dried on the plate for adjusting sample PH level includes, but is not limited to: Formic acid (methanoic acid), Oxalic acid (ethanedioic acid), Lactic acid (2-hydroxypropanoic acid), Malic acid (2-hydroxybutanedioic acid), Citric acid (2-hydroxypropane-1 ,2,3-tricarboxylic acid), Carbonic acid (hydroxymethanoic acid, not an lUPAC name), Aminomethylphosphonic acid.
  • Formic acid methanoic acid
  • Oxalic acid ethanedioic acid
  • Lactic acid (2-hydroxypropanoic acid
  • Malic acid (2-hydroxybutanedioic acid
  • Citric acid (2-hydroxypropane-1 ,2,3-tricarboxylic acid
  • Carbonic acid hydroxymethanoic acid, not an lUPAC name
  • Aminomethylphosphonic acid
  • the second plate comprises spacers that are fixed on the inner surface of the second plate. It should be noted, however, that in some embodiments the spacers are fixed on the inner surface of the first plate and in other embodiments on the inner surfaces of both the second plate and the first plate
  • the spacer is between 1 urn, 2 urn, 5 urn, 10 urn, 20 urn, 50 urn,
  • the diameter of hole in the spacer is around 0.5mm, 1 mm, 2mm, 3 mm 4mm, 5mm, or in a range between any of the two values.
  • the center-to-center spacing between holes is 1 mm, 2mm, 3mm, 4mm, 5mm, 6 mm, 7mm, 8mm, 9mm, 10mm, 20mm, 50mm. or in a range between any of the two values.
  • the second plate is a transparent flat film, with thickness around 1 urn, 2 urn, 5 urn, 10 urn, 20 urn, 50 urn, 100 urn, 200 urn, 500 urn, 1000 urn or in a range between any of the two values.
  • the first plate and the second plate are moveable relative to each other into different configuration.
  • One of the configurations is an open configuration, in which the two plates are partially or entirely separated apart and the spacing between the plates are not regulated by the spacers.
  • Fig. C1 shows the plates in the open configuration, in which a sample, such as but not limited to blood, can be added to first plate, the second plate, or both of the plates.
  • the inner surface of a respective plate comprises a sample contact area, which occupies a part of the entirety of the inner surface.
  • the spacers are positioned within the sample contact area. In some embodiments, the spacers are not fixed to any one of the plates, but are mixed in the sample.
  • the second plate is a transparent thin film with smooth surface. It is necessary that the absorption of second plate does not interfere with the absorption of color indicator. Depends on the flexibility of the material, thickness from 10 urn ⁇ 300 urn can be used as second plate, as long as no distortion of sample chamber will happen after second plate is pressed onto the sample.
  • Fig 3 shows a Schematics of test procedure. 1. First, minute samples are added to each well printed with color indicator and and pH regulating agent. 2. The transparent second plate is then pressed on top of the spacer to form a closed sample chamber. 3. Incubation about 1 min to allow each individual sample to develop color. In this process, the color indicator and pH regulating agent is fully dissolved and mixed.
  • a white polystyrene (PS) substrate printed with home-made color indicator and pH regulating agent.
  • the color indicator and pH regulating agent amount on the sensing area is carefully controlled according to the dimension of the well, so that when each well is filled full with sample, the desired pH level and color indicator concentration can be achieved.
  • different chemicals are used as color indicator.
  • Color Indicator can be: (1) For lead detection, the color indicator is 0.01 % ⁇ 0.2% Sodium Rhodizonate ( preferable 0.2% after dissolved in sample), or (2) For Copper, Cadmium, Chromium, Mercury, 10 uM ⁇ 1 mM Dithizone (preferable 30 uM after dissolved in sample)
  • the printing parameter for Color Indicator agent can vary as long as uniform drying is achieved on the first plate.
  • the printing conditions i.e., droplet volume, speed, depends on the surface wetting property of the first plate, which is well-known to skilled person, thus do not require elucidation.
  • the printing condition is droplet diameter 500 ⁇ 600 urn, pitch ⁇ 1 mm, print speed ⁇ 10mm/sec.
  • the well dimension is determined by dimensions of holes array on the spacer.
  • the thickness of the spacer, the diameter of the holes and their spacing determines the sample volume. Their configuration is flexible but it is crucial to avoid distortion of sample chamber under certain configurations, i.e. small aspect ratio.
  • the thickness of the spacer can be 2 um ⁇ 1 mm (preferably 100 urn)
  • the well diameter can be 100 um ⁇ 10 mm (preferably, 3 mm)
  • the center-to-center spacing can be 100 um ⁇ 10 mm, (preferably, 6 mm).
  • the method of the present invention after step (2) and before step (3), further comprise incubating the layer of uniform thickness for a predetermined period of time.
  • the predetermined period of time is equal to or longer than the time needed for the detection antibody to diffuse into the sample across the layer of uniform thickness.
  • the predetermined period of time is less than 10 seconds, 20 seconds, 30 seconds, 45 seconds, 1 minute, 1.5 minutes, 2 minutes, 3 minutes, 4 minutes, 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 15 minutes, 20 minutes, 30 minutes, or 60 minutes, or in a range between any of the two values.
  • Fig. C4 shows the diagram of a chemical reaction that is used to test lead in water.
  • the lead ion reacts with Sodium Rhodizonate (dark yellow color) dissolved in sample, which form a insoluable lead Rhodizonate that has a red-crimson color.
  • the color absorption can be analyzed to calculate the lead concentration in water.
  • Fig. C5 A diagram of a chemical reaction that is used to test heavy metals in water.
  • the heavy metals can be Cd, Cu, Cr, Hg.
  • the heavy metal ion reacts with Dithiozone dissolved in sample, which form a Dithizone-Metal complex that yield a different color for different heavy metals.
  • the color can be used to identify the type of heavy metals and the color absorption can be analyzed to calculate the heavy metal concentration in water.
  • Fig. C6 shows schematics of converting colorimetric Lead in water test standard curve of individual R, G, B channel to a single standard curve. For each sample contains different concentration of heavy metals, the R, G, B signal are different. A combination of R, G, B channel signal at different Lead concentration is used for this conversion.
  • the method of combination is linear combination.
  • the coefficient for combining RGB channel signal is a constant.
  • the coefficient for combining RGB channel signal is a matrix.
  • the coefficient for combining RGB channel signal is a function of lead concentration in water.
  • the algorithm to converting standard curve of individual R, G, B channel to a single standard curve is a process to find the best coefficient of combing R,G,B signals so that best sensitivity of assay can be achieved.
  • a linear combination of R, G, B channel signal at different Lead concentration is used for this conversion.
  • the linear coefficient is trained using a Generalized Reduced Gradient Algorithm. Such algorithm is open source and known to skilled person and does not require elucidation.
  • the process of this algorithm is shown in a diagram, briefly:
  • each plate has 48 wells, well diameter is 3 mm Center-to-center distance is 6 mm, well height is -100 um (controlled using double- sided tape from Adhesive Research).
  • the light source used is the smartphone camera flash light. And the image is taken using the smartphone's camera.
  • Fig. C8 shows the Lead in water test standard curve of individual R, G, B channel.
  • the converted data is fitted with 5PL logistic fitting. Error bar is Standard deviation of 6 replicate wells.
  • the LOD, after conversion is 8.5 ppb.
  • Fig. C9 shows the sensitivity of all 8 different test plates in this example of the invention. Each test plate is prepared separately with different reagent and tested at different time. The average LOD achieved is 8 ppb, which is below the EPA action level at 15 ppb.
  • Fig. C10 Table of Intra-assay, Inter Assay and Day-to-day CV% of lead in water test.
  • this example shows a test of lead concentration in tap water that shows (1) Sensitivity: average LOD ⁇ 8 ppb. All test plates show LOD that meets EPA standard (15 ppb), with the best LOD achieved is 3.9 ppb. (2) Repeatability: Intra-assay CV% at LOD ⁇ 4%, Inter-assay CV% at LOD ⁇ 4% and Day-to-day CV% at LOD - 1.1 %
  • D Foodstuff Safety and Allergen Test Using QMAX Device Another aspect of the present invention provides devices and methods for safety and allergen test in foodstuff samples.
  • the devices, systems and methods in the present invention may find use in analyzing a foodstuff sample, e.g., a sample from raw food, processed food, cooked food, drinking water, etc., for the presence of foodstuff markers.
  • a foodstuff marker may be any suitable marker, such as those shown in Table B9, below, that can be captured by a capturing agent that specifically binds the foodstuff marker in a CROF device configured with the capturing agent.
  • the environmental sample may be obtained from any suitable source, such as tap water, drinking water, prepared food, processed food or raw food, etc.
  • the presence or absence, or the quantitative level of the foodstuff marker in the sample may be indicative of the safety or harmfulness to a subject if the food stuff is consumed.
  • the foodstuff marker is a substance derived from a pathogenic or microbial organism that is indicative of the presence of the organism in the foodstuff from which the sample was obtained.
  • the foodstuff marker is a toxic or harmful substance if consumed by a subject.
  • the foodstuff marker is a bioactive compound that may unintentionally or unexpectedly alter the physiology if consumed by the subject.
  • the foodstuff marker is indicative of the manner in which the foodstuff was obtained (grown, procured, caught, harvested, processed, cooked, etc.).
  • the foodstuff marker is indicative of the nutritional content of the foodstuff.
  • the foodstuff marker is an allergen that may induce an allergic reaction if the foodstuff from which the sample is obtained is consumed by a subject.
  • the devices, systems and methods in the present invention further includes receiving or providing a report that indicates the safety or harmfulness for a subject to consume the food stuff from which the sample was obtained based on information including the measured level of the foodstuff marker.
  • the information used to assess the safety of the foodstuff for consumption may include data other than the type and measured amount of the foodstuff marker. These other data may include any health condition associated with the consumer (allergies, pregnancy, chronic or acute diseases, current prescription medications, etc.).
  • the report may be generated by the device configured to read the CROF device, or may be generated at a remote location upon sending the data including the measured amount of the foodstuff marker.
  • a food safety expert may be at the remote location or have access to the data sent to the remote location, and may analyze or review the data to generate the report.
  • the food safety expert may be a scientist or administrator at a governmental agency, such as the US Food and Drug Administration (FDA) or the CDC, a research institution, such as a university, or a private company.
  • the food safety expert may send to the user instructions or recommendations based on the data transmitted by the device and/or analyzed at the remote location.
  • the QMAX device is used to detect the presence and/or quantity of analyte, including, but not limited to, the foodstuff markers listed in Table D1.
  • Toxoplasma gondii Vibrio cholera, Vibrio parahaemolyticus, Vibrio vulnificus, Enterococcus faecalis, Enterococcus faecium,
  • Angiostrongylus Cantonensis Cyclospora cayetanensis
  • Entamoeba histolytica Trichinella spiralis
  • BMAA N-methylamino-L-alanine
  • neurotoxins BoNT A, B, Ricin A, B; diphtheria toxin; Aristolochic acid; Colchicine, Ochratoxin A, Sterigmatocystin, Ergotamine, Fumonisins, Fusarin C, domoic acid, Brevetoxin, Mycotoxins, Antimony, Ciguatera fish poisoning, museinol, muscarine, psilocybin, coprius artemetrais, ibotenic acid, amanitin, Nitrite poisoning, Puffer fish (tetrodotoxin), histamine, amnesic,
  • Heavy metals Lead, mercury, cadmium, Chromium, Arsenic, Copper, Tin, Zinc,
  • Mald1.0104 Mald1.0105, Mald1.0106, Mald1.0107, Mald1.0108, Mald1.0109, Mald1.0201, Mald1.0202, Mald1.0203, Mald1.0204, Mald1.0205, Mald1.0206, Mald1.0207, Mald1.0208, Mald1.0301, Mald1.0302, Mald1.0303, Mald1.0304, Mald1.0401, Mald1.0402, Mald1.0403, Mald3.0101w, Mald3.0102w, Mald3.0201w,
  • Prunus persica Prup4.0101 , Prup4.0201
  • EPN fenitrothion, pirimiphos-methyl, thiabendazole, methiocarb, Carbendazim, deltamethrin, Avermectin, Carbaryl, Cyanazine, Kresoxim, resmethrin, kadethrin, cyhalothrin, biphenthrin, fenpropathrin, allethrin and tralomethrin; aromatic-substituted alkanecarboxylic acid esters such as fenvarerate, flucythrinate, fluvalinate and cycloprothrin; and non-ester compounds such as etofenprox, halfenprox (MTI-732), 1-(3-phenoxyphenyl)-4-(4- ethoxyphenyl)-4-methylpentane (MTI-790), 1-(3-phenoxy-4- fluorophenyl)-4-(4-ethoxyphenyl)-4-methylpentane
  • Herbicide atrazine deethylatrazine, cyanazine, terbuthylazine, terbutryn, molinate, simazine, prometon, promteryn, hydroxyatrazine, 2,6- dichlorobenzamide (BAM), N-dealkylated triazines, mecoprop, thiram, acetochlor, alachlor, Chlorothalonil, Chlorsulfuron, Fenoxaprop ethyl, Linuron, monuron, diuron, Quizalofop-ethyl, Imazalil, Iprodione, Iprovalicarb, Myclobutanil
  • Antibiotics 3-Amino-5-morpholinomethyl-2-oxazolidone AMOZ; tissue bound metabolite of furaltadone), oxytetracycline, rolitetracycline, Actinomycin D, Amikacin sulfate, Aminoglycosides, nitrofuran (AOZ), Chloramphenicol, Doxycycline, Streptomycin, gentamicin, Source/Class Marker/target
  • neomycin kanamycin, sulfamethazine, enrofloxacin, sulfadiazine, enrofloxacin
  • Nutritional content Vitamins A (retinol), B12 (cobalmins), B6 (pyridoxine), B1 (thiamin),
  • B2 riboflavin
  • B3 niacin
  • B5 D-pantothenic acid
  • B7 biotin
  • B9 folic acid
  • the imprecise force is around 0.01 kg, 0.05 kg, 0.1 kg, 0.25 kg, 0.5 kg, 1 kg, 2.5 kg, 5 kg, 7.5 kg, 10 kg, 20 kg, 25 kg, 30 kg, 40 kg, 50 kg, 60 kg, 70 kg, 80 kg, 100 kg, 200 kg, or in a range between any two of these values; and a preferred range of 0.5 - 2 kg, 2 - 5 kg, 5 - 7.5 kg, 7.5 - 10 kg, 10 - 20 kg, 20 - 40 kg, 40 - 60 kg, or 60 - 100 kg.
  • the imprecise force is applied by human hand, for example, e.g., by pinching an object together between a thumb and index finger, or by pinching and rubbing an object together between a thumb and index finger.
  • the hand pressing force is around 0.05 kg, 0.1 kg, 0.25 kg, 0.5 kg, 1 kg, 2.5 kg, 5 kg, 7.5 kg, 10 kg, 20 kg, 25 kg, 30 kg, 40 kg, 50 kg, 60 kg, or in a range between any two of these values; and a preferred range of 0.5 - 1 kg, 1 - 2 kg, 2- 4 kg, 4 - 6 kg, 6 - 10 kg, 10 - 20 kg, 20 - 40 kg, or 40 - 60 kg.
  • the hand pressing has a pressure of 0.01 kg/cm 2 , 0.1 kg/cm 2 , 0.5 kg/cm 2 , 1 kg/cm 2 , 2 kg/cm 2 , 2.5 kg/cm 2 , 5 kg/cm 2 , 10 kg/cm 2 , 20 kg/cm 2 , 30 kg/cm 2 , 40 kg/cm 2 , 50 kg/cm 2 , 60 kg/cm 2 , 100 kg/cm 2 , 150 kg/cm 2 , 200 kg/cm 2 , or a range between any two of the values; and a preferred range of 0.1 kg/cm 2 to 0.5 kg/cm 2 , 0.5 kg/cm 2 to 1 kg/cm 2 , 1 kg/cm 2 to 5 kg/cm 2 , or 5 kg/cm 2 to 10 kg/cm 2 .
  • the term "imprecise” in the context of a force refers to a force that (a) has a magnitude that is not precisely known or precisely predictable at the time the force is applied;
  • the imprecision (i.e. the variation) of the force in (a) and (c) is at least 20% of the total force that actually is applied.
  • An imprecise force can be applied by human hand, for example, e.g., by pinching an object together between a thumb and index finger, or by pinching and rubbing an object together between a thumb and index finger.
  • a device for forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising:
  • one or both plates are flexible
  • each of the plates comprises an inner surface that has a sample contact area for contacting a fluidic sample
  • each of the plates comprises, on its respective outer surface, a force area for applying an imprecise pressing force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, and a predetermined fixed inter- spacer-distance;
  • the fourth power of the inter-spacer-distance (IDS) divided by the thickness (h) and the Young's modulus (E) of the flexible plate (ISD 4 /(hE)) is 5x10 6 um 3 /GPa or less;
  • At least one of the spacers is inside the sample contact area
  • one of the configurations is an open configuration, in which: the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers, and the sample is deposited on one or both of the plates;
  • another of the configurations is a closed configuration which is configured after the sample is deposited in the open configuration and the plates are forced to the closed configuration by applying the imprecise pressing force on the force area; and in the closed configuration: at least part of the sample is compressed by the two plates into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers.
  • a method of forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising the steps of:
  • the plates are movable relative to each other into different configurations; ii. one or both plates are flexible;
  • each of the plates comprises an inner surface that has a sample contact area for contacting a fluidic sample
  • each of the plates comprises, on its respective outer surface, a force area for applying an imprecise pressing force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, and a predetermined fixed inter-spacer-distance; vii. the fourth power of the inter-spacer-distance (IDS) divided by the thickness (h) and the Young's modulus (E) of the flexible plate (ISD 4 /(hE)) is 5x10 6 um 3 /GPa or less; and
  • At least one of the spacers is inside the sample contact area
  • conformable pressing either in parallel or sequentially, an area of at least one of the plates to press the plates together to a closed configuration, wherein the conformable pressing generates a substantially uniform pressure on the plates over the at least part of the sample, and the pressing spreads the at least part of the sample laterally between the sample contact surfaces of the plates, and wherein the closed configuration is a configuration in which the spacing between the plates in the layer of uniform thickness region is regulated by the spacers; and wherein the reduced thickness of the sample reduces the time for mixing the reagents on the storage site with the sample, and
  • the force that presses the two plates into the closed configuration is an imprecise pressing force provided by human hand.
  • a device for forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise force comprising:
  • the plates are movable relative to each other into different configurations
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, a predetermined width, and a predetermined inter-spacer-distance;
  • a ratio of the inter-spacer-distance to the spacer width is 1.5 or larger
  • At least one of the spacers is inside the sample contact area
  • one of the configurations is an open configuration, in which: the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers, and the sample is deposited on one or both of the plates;
  • another of the configurations is a closed configuration which is configured after the sample deposition in the open configuration; and in the closed configuration: at least part of the sample is compressed by the two plates into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers; and wherein the force that presses the two plates into the closed configuration is an imprecise pressing force provided by human hand.
  • a method of forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising the steps of:
  • the plates are movable relative to each other into different configurations
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, a predetermined width, and a predetermined inter-spacer-distance;
  • a ratio of the inter-spacer-distance to the spacer width is 1.5 or larger
  • At least one of the spacers is inside the sample contact area
  • conformable pressing either in parallel or sequentially, an area of at least one of the plates to press the plates together to a closed configuration, wherein the conformable pressing generates a substantially uniform pressure on the plates over the at least part of the sample, and the pressing spreads the at least part of the sample laterally between the sample contact surfaces of the plates, and wherein the closed configuration is a configuration in which the spacing between the plates in the layer of uniform thickness region is regulated by the spacers; and wherein the reduced thickness of the sample reduces the time for mixing the reagents on the storage site with the sample, and
  • the force that presses the two plates into the closed configuration is an imprecise pressing force provided by human hand.
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample; iv. each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, a predetermined width, and a predetermined inter-spacer-distance;
  • a ratio of the inter-spacer-distance to the spacer width is 1.5 or larger
  • At least one of the spacers is inside the sample contact area
  • one of the configurations is an open configuration, in which: the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers, and the sample is deposited on one or both of the plates;
  • another of the configurations is a closed configuration which is configured after the sample deposition in the open configuration; and in the closed configuration: at least part of the sample is compressed by the two plates into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
  • a method of forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising the steps of:
  • the plates are movable relative to each other into different configurations
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, a predetermined width, and a predetermined inter-spacer-distance;
  • a ratio of the inter-spacer-distance to the spacer width is 1.5 or larger
  • At least one of the spacers is inside the sample contact area
  • conformable pressing either in parallel or sequentially, an area of at least one of the plates to press the plates together to a closed configuration, wherein the conformable pressing generates a substantially uniform pressure on the plates over the at least part of the sample, and the pressing spreads the at least part of the sample laterally between the sample contact surfaces of the plates, and wherein the closed configuration is a configuration in which the spacing between the plates in the layer of uniform thickness region is regulated by the spacers; and wherein the reduced thickness of the sample reduces the time for mixing the reagents on the storage site with the sample, and
  • the force that presses the two plates into the closed configuration is an imprecise pressing force provided by human hand.
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample; iv. each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, a predetermined width, and a predetermined inter-spacer-distance;
  • a ratio of the inter-spacer-distance to the spacer width is 1.5 or larger.
  • at least one of the spacers is inside the sample contact area; and wherein one of the configurations is an open configuration, in which: the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers, and the sample is deposited on one or both of the plates;
  • another of the configurations is a closed configuration which is configured after the sample deposition in the open configuration; and in the closed configuration: at least part of the sample is compressed by the two plates into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
  • a method of forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising the steps of: (a) obtaining a first plate, a second plate, and spacers, wherein:
  • the plates are movable relative to each other into different configurations
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, a predetermined width, and a predetermined inter-spacer-distance;
  • a ratio of the inter-spacer-distance to the spacer width is 1.5 or larger. viii. at least one of the spacers is inside the sample contact area;
  • conformable pressing either in parallel or sequentially, an area of at least one of the plates to press the plates together to a closed configuration, wherein the conformable pressing generates a substantially uniform pressure on the plates over the at least part of the sample, and the pressing spreads the at least part of the sample laterally between the sample contact surfaces of the plates, and wherein the closed configuration is a configuration in which the spacing between the plates in the layer of uniform thickness region is regulated by the spacers; and wherein the reduced thickness of the sample reduces the time for mixing the reagents on the storage site with the sample, and
  • imprecise force provided by human hand comprising:
  • a first plate a second plate, spacers, and an area-determination device, wherein: i. the plates are movable relative to each other into different configurations;
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample that has a relevant volume to be measured;
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, and a predetermined constant inter- spacer-distance;
  • ISD 4 /(hE) is 5x10 6 um 3 /GPa or less.
  • the area-determination device is configured to determine the lateral area of the relevant volume
  • one of the configurations is an open configuration, in which: the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers, and the sample is deposited on one or both of the plates;
  • another of the configurations is a closed configuration which is configured after the sample deposition in the open configuration; and in the closed configuration: at least part of the sample is compressed by the two plates into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
  • the relevant volume of the sample is a partial or entire volume of the uniform thickness layer and the value of the relevant volume is determined by the uniform thickness and the determined lateral area;
  • the area-determination device comprises an area in the sample contact area of a plate, wherein the area is less than 1/100, 1/20, 1/10, 1/6, 1/5, 1/4, 1/3, 1/2, 2/3 of the sample contact area, or in a range between any of the two values.
  • the area-determination device comprises a camera and an area in the sample contact area of a plate, wherein the area is in contact with the sample.
  • a method of forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising the steps of:
  • the plates are movable relative to each other into different configurations
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample that has a relevant volume to be measured;
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, and a predetermined constant inter- spacer-distance;
  • thickness (h) and the Young's modulus (E) of the flexible plate (ISD 4 /(hE)) is 5x10 6 um 3 /GPa or less.
  • the area-determination device is configured to determine the lateral area of the relevant volume
  • conformable pressing either in parallel or sequentially, an area of at least one of the plates to press the plates together to a closed configuration, wherein the conformable pressing generates a substantially uniform pressure on the plates over the at least part of the sample, and the pressing spreads the at least part of the sample laterally between the sample contact surfaces of the plates, and wherein the closed configuration is a configuration in which the spacing between the plates in the layer of uniform thickness region is regulated by the spacers; and wherein the reduced thickness of the sample reduces the time for mixing the reagents on the storage site with the sample, and
  • the force that presses the two plates into the closed configuration is an imprecise pressing force provided by human hand.
  • a device for determining a relevant sample volume by pressing with an imprecise force provided by human hand comprising:
  • the plates are movable relative to each other into different configurations
  • one or both plates are flexible
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample that has a relevant volume to be measured;
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, and a predetermined constant inter- spacer-distance;
  • ISD 4 /(hE) is 5x10 6 um 3 /GPa or less.
  • at least one of the spacers is inside the sample contact area; and ix. the area-determination device is configured to determine the lateral area of the relevant volume;
  • one of the configurations is an open configuration, in which: the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers, and the sample is deposited on one or both of the plates;
  • another of the configurations is a closed configuration which is configured after the sample deposition in the open configuration; and in the closed configuration: at least part of the sample is compressed by the two plates into a layer of highly uniform thickness and is substantially stagnant relative to the plates, wherein the uniform thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
  • the relevant volume of the sample is a partial or entire volume of the uniform thickness layer and the value of the relevant volume is determined by the uniform thickness and the determined lateral area;
  • a method of forming a thin fluidic sample layer with a uniform predetermined thickness by pressing with an imprecise pressing force comprising the steps of:
  • the plates are movable relative to each other into different configurations
  • each of the plates comprises, on its respective inner surface, a sample contact area for contacting and/or compressing a fluidic sample that has a relevant volume to be measured;
  • each of the plates comprises, on its respective outer surface, an area for applying a force that forces the plates together;
  • one or both of the plates comprise the spacers that are permanently fixed on the inner surface of a respective plate;
  • the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, and a predetermined constant inter- spacer-distance;
  • the thickness (h) and the Young's modulus (E) of the flexible plate (ISD 4 /(hE)) is 5x10 6 um 3 /GPa or less.
  • at least one of the spacers is inside the sample contact area; and ix. the area-determination device is configured to determine the lateral area of the relevant volume;
  • conformable pressing either in parallel or sequentially, an area of at least one of the plates to press the plates together to a closed configuration, wherein the conformable pressing generates a substantially uniform pressure on the plates over the at least part of the sample, and the pressing spreads the at least part of the sample laterally between the sample contact surfaces of the plates, and wherein the closed configuration is a configuration in which the spacing between the plates in the layer of uniform thickness region is regulated by the spacers; and wherein the reduced thickness of the sample reduces the time for mixing the reagents on the storage site with the sample, and
  • the force that presses the two plates into the closed configuration is an imprecise pressing force provided by human hand.
  • imprecise force refers to a force that has a magnitude that is completely unknown, known only in a magnitude range but not in a particular magnitude value (the magnitude range varies at least 20% from the minimum to the maximum of the range), or unpredictable at the time that a force is applied.
  • Examples of an imprecise force include that the magnitude of an imprecise force may vary from one application of the force to the next, may be uneven across the area upon which the force is applied, and may vary over the time that the force is being applied. An imprecise force does not need to be measured at the time that it is applied.
  • the deformable sample is a fluidic sample.
  • the deformable sample is a liquid sample.
  • the imprecision force has a variation at least 20%, 30%, 40%, 50%, 60, 70%, 80%, 90% 100%, 150%, 200%, 300%, 500%, or in a range of any two values, of the total force that actually is applied.
  • the device is further configured to have, after the pressing force is removed, a sample thickness that is substantially the same in thickness and uniformity as that when the force is applied.
  • the highly uniform layer has a thickness that varies by less than 15 %, 10%, or 5% of an average thickness.
  • Another aspect of the present invention provides devices and methods for bio/chemical assays using QMAX device in which binding site and storage site are on the same plate, meaning both capture agent and second agent are coated on the same plate.
  • a method for assaying a sample comprising
  • first plate and second plate are movable relative to each other into different configurations, including an open and a closed configurations
  • FB A device for performing a competitive assay comprising:
  • a first plate comprising, on its inner surface, a sample contact area for contacting a sample that contains a target analyte; a second plate comprising a sample contact area that comprises an assaying area, wherein the assaying area comprises
  • first plate and second plate are movable relative to each other into different configurations
  • one of the configurations is an open configuration, in which the plates are partially or entirely separated apart, and the average spacing between the sample contact areas of the plates is larger than 300 urn;
  • another configuration is a closed configuration in which the average spacing between the sample contact areas of the plates is 200 ⁇ or less.
  • the method or device of any prior embodiment wherein the capture agents and the second agents are separated by a distance that is at least 2 times less than the average spacing between the sample contact area of the two plates.
  • the method or device of any prior embodiment wherein the capture agents and the second agents are separated by a distance that is at least 2 times, 3 times, 5 times, 10 times, 20 times, 30 times, 50 times, 100 times, 200 times,300 times, 500 times, 1000 times, 2000 times, 5000 times, 10000 times, 5000 times, less than the average spacing between the sample contact area of the two plates, or in a range of any two values.
  • the signal related to the analyte captured by the capture agent are the signals coming from (i) the analyte captured by the capture agent, (ii) the label attached an analyte that is captured by the binding site, or (iii) both (i) and (ii).
  • the method or device of any prior embodiment wherein one or both of the sample contact areas comprise spacers, wherein the spacers regulate the spacing between the sample contact areas of the plates when the plates are in the closed configuration.
  • the device further comprises spacers that regulate the spacing between the sample contact areas when the plates are in a closed configuration.
  • the storage site further comprises another reagent.
  • binding site comprises, in addition to immobilized capture agent, another reagent that is, upon contacting the sample, capable of diffusion in the sample,
  • sample is a blood sample (whole blood, plasma, or serum).
  • the material of fluorescent microsphere is dielectric, (e.g. Si02, Polystyrene,) or the combination of dielectric materials thereof.
  • the exemplary assay recipes disclosed herein are applicable to embodiments including but not limited to: bio/chemical assays, QMAX cards and systems, QMAX with hinges, notches, recessed edges and sliders, assays and devices with uniform sample thickness, smartphone detection systems, cloud computing designs, various detection methods, labels, capture agents and detection agents, analytes, diseases, applications, and samples; the various embodiments are disclosed, described, and/or referred to in the aforementioned applications, all of which are hereby incorporated in reference by their entireties.
  • the present invention includes a variety of embodiments, which can be combined in multiple ways as long as the various components do not contradict one another.
  • the embodiments should be regarded as a single invention file: each filing has other filing as the references and is also referenced in its entirety and for all purpose, rather than as a discrete independent. These embodiments include not only the disclosures in the current file, but also the documents that are herein referenced, incorporated, or to which priority is claimed.
  • CROF Card or card
  • COF Card or card
  • COF Card QMAX-Card
  • Q-Card CROF device
  • COF device COF device
  • QMAX-device CROF plates
  • COF plates COF plates
  • QMAX-plates are interchangeable, except that in some embodiments, the COF card does not comprise spacers; and the terms refer to a device that comprises a first plate and a second plate that are movable relative to each other into different configurations (including an open configuration and a closed configuration), and that comprises spacers (except some embodiments of the COF card) that regulate the spacing between the plates.
  • X-plate refers to one of the two plates in a CROF card, wherein the spacers are fixed to this plate. More descriptions of the COF Card, CROF Card, and X-plate are given in the provisional application serial nos. 62/456065, filed on February 7, 2017, which is incorporated herein in its entirety for all purposes.
  • the devices, systems, and methods herein disclosed can include or use Q-cards, spacers, and uniform sample thickness embodiments for sample detection, analysis, and quantification.
  • the Q-card comprises spacers, which help to render at least part of the sample into a layer of high uniformity.
  • the structure, material, function, variation and dimension of the spacers, as well as the uniformity of the spacers and the sample layer, are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on February 8, 2017, all of which applications are incorporated herein in their entireties for all purposes.
  • the devices, systems, and methods herein disclosed can include or use Q-cards for sample detection, analysis, and quantification.
  • the Q-card comprises hinges, notches, recesses, and sliders, which help to facilitate the manipulation of the Q card and the measurement of the samples.
  • the structure, material, function, variation and dimension of the hinges, notches, recesses, and sliders are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on February 8, 2017, all of which applications are incorporated herein in their entireties for all purposes.
  • the sample contact area of one or both of the plates comprises a compressed open flow monitoring surface structures (MSS) that are configured to monitoring how much flow has occurred after COF.
  • MSS comprises, in some embodiments, shallow square array, which will cause friction to the components (e.g. blood cells in a blood) in a sample.
  • the depth of the MSS can be 1/1000, 1/100, 1/100, 1/5, 1/2 of the spacer height or in a range of any two values, and in either protrusion or well form.
  • the devices, systems, and methods herein disclosed can include or use Q-cards for sample detection, analysis, and quantification.
  • the Q-cards are used together with sliders that allow the card to be read by a smartphone detection system.
  • the structure, material, function, variation, dimension and connection of the Q-card, the sliders, and the smartphone detection system are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on February 8, 2017, all of which applications are incorporated herein in their entireties for all purposes. (5) Detection methods
  • the devices, systems, and methods herein disclosed can include or be used in various types of detection methods.
  • the detection methods are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14,
  • the devices, systems, and methods herein disclosed can employ various types of labels, capture agents, and detection agents that are used for analytes detection.
  • the labels are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on February 8, 2017, all of which applications are incorporated herein in their entireties for all purposes.
  • the devices, systems, and methods herein disclosed can be applied to manipulation and detection of various types of analytes (including biomarkers).
  • the analytes and are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8,
  • the devices, systems, and methods herein disclosed can be used for various applications (fields and samples).
  • the applications are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on February 8, 2017, all of which applications are incorporated herein in their entireties for all purposes. (9) Cloud
  • the devices, systems, and methods herein disclosed can employ cloud technology for data transfer, storage, and/or analysis.
  • the related cloud technologies are herein disclosed, or listed, described, and summarized in PCT Application (designating U.S.) Nos. PCT/US2016/045437 and PCT/US0216/051775, which were respectively filed on August 10, 2016 and September 14, 2016, US Provisional Application No. 62/456065, which was filed on February 7, 2017, US Provisional Application No. 62/456287, which was filed on February 8, 2017, and US Provisional Application No. 62/456504, which was filed on February 8, 2017, all of which applications are incorporated herein in their entireties for all purposes. Additional Notes
  • adapted and “configured” mean that the element, component, or other subject matter is designed and/or intended to perform a given function.
  • the use of the terms “adapted” and “configured” should not be construed to mean that a given element, component, or other subject matter is simply “capable of” performing a given function.
  • subject matter that is recited as being configured to perform a particular function may additionally or alternatively be described as being operative to perform that function.
  • the phrase, "for example,” the phrase, “as an example,” and/or simply the terms “example” and “exemplary” when used with reference to one or more components, features, details, structures, embodiments, and/or methods according to the present disclosure, are intended to convey that the described component, feature, detail, structure, embodiment, and/or method is an illustrative, non-exclusive example of components, features, details, structures, embodiments, and/or methods according to the present disclosure.
  • the phrases "at least one of” and “one or more of,” in reference to a list of more than one entity, means any one or more of the entity in the list of entity, and is not limited to at least one of each and every entity specifically listed within the list of entity.
  • “at least one of A and B” (or, equivalently, “at least one of A or B,” or, equivalently, “at least one of A and/or B”) may refer to A alone, B alone, or the combination of A and B.
  • the term "and/or" placed between a first entity and a second entity means one of (1) the first entity, (2) the second entity, and (3) the first entity and the second entity.
  • Multiple entity listed with “and/or” should be construed in the same manner, i.e., "one or more" of the entity so conjoined.
  • Other entity may optionally be present other than the entity specifically identified by the "and/or” clause, whether related or unrelated to those entities specifically identified.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne, entre autres, certaines surfaces et certains porte-échantillons permettant d'améliorer la sensibilité, la vitesse et la facilité d'utilisation dans des dosages basés sur des signaux optiques, tels que des dosages colorimétriques ou des dosages par fluorescence.
PCT/US2018/018521 2017-02-16 2018-02-16 Dosage à surface texturée WO2018152422A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US16/485,347 US10966634B2 (en) 2017-02-16 2018-02-16 Assay with textured surface
CA3053301A CA3053301A1 (fr) 2017-02-16 2018-02-16 Dosage a surface texturee
CN201880025156.1A CN111448449A (zh) 2017-02-16 2018-02-16 采用纹理化表面的测定
JP2019544634A JP7107953B2 (ja) 2017-02-16 2018-02-16 テクスチャ表面を用いたアッセイ

Applications Claiming Priority (48)

Application Number Priority Date Filing Date Title
US201762460062P 2017-02-16 2017-02-16
US201762460076P 2017-02-16 2017-02-16
US201762459920P 2017-02-16 2017-02-16
US201762460047P 2017-02-16 2017-02-16
US201762459972P 2017-02-16 2017-02-16
US201762460069P 2017-02-16 2017-02-16
US201762460091P 2017-02-16 2017-02-16
US201762460083P 2017-02-16 2017-02-16
US201762460088P 2017-02-16 2017-02-16
US201762460075P 2017-02-16 2017-02-16
US62/460,075 2017-02-16
US62/460,088 2017-02-16
US62/460,076 2017-02-16
US62/460,083 2017-02-16
US62/460,091 2017-02-16
US62/460,062 2017-02-16
US62/459,920 2017-02-16
US62/460,047 2017-02-16
US62/459,972 2017-02-16
US62/460,069 2017-02-16
USPCT/US2018/017307 2018-02-07
PCT/US2018/017307 WO2018148342A1 (fr) 2017-02-07 2018-02-07 Dosage et utilisation d'écoulement ouvert comprimé
USPCT/US2018/017492 2018-02-08
USPCT/US2018/017501 2018-02-08
PCT/US2018/017501 WO2018148469A1 (fr) 2017-02-08 2018-02-08 Extraction et dosage de matières bio/chimiques
PCT/US2018/017489 WO2018148458A1 (fr) 2017-02-08 2018-02-08 Dosage numérique
USPCT/US2018/017499 2018-02-08
PCT/US2018/017502 WO2018148470A1 (fr) 2017-02-08 2018-02-08 Collecte et manipulation d'échantillons pour analyse retardée
USPCT/US2018/017504 2018-02-08
PCT/US2018/017494 WO2018148463A1 (fr) 2017-02-08 2018-02-08 Dosage d'hybridation d'acide nucléique
USPCT/US2018/017489 2018-02-08
USPCT/US2018/017494 2018-02-08
USPCT/US2018/017502 2018-02-08
PCT/US2018/017492 WO2018148461A1 (fr) 2017-02-09 2018-02-08 Dosage avec amplification
PCT/US2018/017504 WO2018148471A2 (fr) 2017-02-08 2018-02-08 Optique, dispositif et système d'essai
USPCT/US2018/017716 2018-02-09
USPCT/US2018/017713 2018-02-09
PCT/US2018/017713 WO2018148607A1 (fr) 2017-02-09 2018-02-09 Dosage utilisant différentes hauteurs d'espacement
PCT/US2018/017716 WO2018148609A2 (fr) 2017-02-09 2018-02-09 Dosages colorimétriques
USPCT/US2018/017712 2018-02-09
PCT/US2018/017712 WO2018148606A1 (fr) 2017-02-09 2018-02-09 Dosage et applications qmax (ii)
PCT/US2018/018007 WO2018148729A1 (fr) 2017-02-08 2018-02-13 Dispositifs et procédés de dosage à base de carte qmax
USPCT/US2018/018007 2018-02-13
PCT/US2018/018108 WO2018148764A1 (fr) 2017-02-08 2018-02-14 Manipulation moléculaire et dosage à température contrôlée
PCT/US2018/017499 WO2018152005A1 (fr) 2017-02-08 2018-02-14 Dosages qmax et applications
USPCT/US2018/018108 2018-02-14
USPCT/US2018/018405 2018-02-15
PCT/US2018/018405 WO2018152351A1 (fr) 2017-02-15 2018-02-15 Dosage à changement rapide de température

Related Parent Applications (1)

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PCT/US2018/017502 Continuation WO2018148470A1 (fr) 2017-02-08 2018-02-08 Collecte et manipulation d'échantillons pour analyse retardée

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WO2018152422A1 true WO2018152422A1 (fr) 2018-08-23

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PCT/US2018/018521 WO2018152422A1 (fr) 2017-02-16 2018-02-16 Dosage à surface texturée

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WO (1) WO2018152422A1 (fr)

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