WO2018087301A1 - Procédés pour améliorer la résistance à la sécheresse dans les plantes kgdr06, kgdr26, kgdr25, kgdr42 et kgdr37 - Google Patents

Procédés pour améliorer la résistance à la sécheresse dans les plantes kgdr06, kgdr26, kgdr25, kgdr42 et kgdr37 Download PDF

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WO2018087301A1
WO2018087301A1 PCT/EP2017/078909 EP2017078909W WO2018087301A1 WO 2018087301 A1 WO2018087301 A1 WO 2018087301A1 EP 2017078909 W EP2017078909 W EP 2017078909W WO 2018087301 A1 WO2018087301 A1 WO 2018087301A1
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protein
seq
plant
nos
sequence identity
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PCT/EP2017/078909
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Anne DESLATTES MAYS
Jesse David MUNKVOLD
Matthew Vitabile DILEO
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Keygene N.V.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Definitions

  • Abiotic stresses such as drought, salinity, extreme temperatures, chemical toxicity and oxidative stress, are threats to agriculture and are the primary cause of crop loss worldwide.
  • There have been reports in the art dealing with the biochemical, molecular and genetic background of abiotic stress Wang et al., Planta, 2003, 218(1 ):1-14; Kilian et al., Plant J., 2007, 50(2):347-363).
  • Plant modification to deal with abiotic stress is often based on manipulation of genes that protect and maintain the function and structure of cellular components.
  • due to the genetically complex responses to abiotic stress conditions such plants appear to be more difficult to control and engineer.
  • WO 2003/020015 discloses transgenic plants that are resistant to salt stress by utilizing 9-cis- epoxycarotenoid dioxygenase nucleic acids and polypeptides.
  • Plants with increased drought tolerance are disclosed in, for example, US 2009/0144850, US 2007/0266453, and WO 2002/083911.
  • US2009/0144850 describes a plant displaying a drought tolerance phenotype due to altered expression of a DR02 nucleic acid.
  • US 2007/0266453 describes a plant displaying a drought tolerance phenotype due to altered expression of a DR03 nucleic acid.
  • WO 2002/083911 describes a plant having an increased tolerance to drought stress due to a reduced activity of an ABC transporter which is expressed in guard cells.
  • At least one aspect of the invention described herein relates to a method for improving drought resistance in plants, comprising overexpressing or expressing de novo a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, in a plant, plant protoplast or plant cell, wherein the KGDR06 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39- 43, 45, 47, 49, 51 , and 53, wherein the KGDR26 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89, wherein the KGDR25 protein comprises an amino acid sequence
  • the method comprises genetically engineering the plant, plant protoplast or plant cell to overexpress or express de novo the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • the method comprises transforming a plant protoplast or plant cell with a vector comprising a recombinant nucleic acid encoding the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • the method comprises contacting the plant protoplast or plant cell with an Agrobacterium strain comprising the vector to introduce the recombinant nucleic acid into the plant protoplast or plant cell.
  • the method further comprises regenerating the plant protoplast or plant cell into a plant.
  • the method further comprises testing the plant, plant protoplast or plant cell for overexpression or de novo expression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • the method further comprises testing the plant, plant protoplast or plant cell for improved drought resistance.
  • the method further comprises producing progenies of the plant, plant protoplast or plant cell and selecting one or more progenies that overexpress or express de novo the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • the method further comprises producing progenies of the plant, plant protoplast or plant cell and selecting one or more progenies that have improved drought resistance.
  • the KGDR06 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27,
  • the KGDR06 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 28,
  • the KGDR06 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 28, 30, 32, 34, 36, 38, 44, 46, 48, 50, 52, and 54.
  • the KGDR26 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the KGDR26 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the KGDR26 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the KGDR25 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the KGDR25 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the KGDR25 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the KGDR42 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the KGDR42 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the KGDR42 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the KGDR37 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142,
  • the KGDR37 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 127, 129, 131 , 133, 135, 137, 139, 141 , 143,
  • the KGDR37 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 127, 129, 131 , 133, 135, 137, 139, 141 , 143, 145, 147, 149, 155, 157, 163, 165, 167 and 169.
  • the plant, plant protoplast or plant cell is selected from the group consisting of Solanum tuberosum, Zea mays, Triticum spp. , Triticum aestivum, Oryza sativa, Sorghum bicolor, Dioscorea spp. , Musa spp. , Manihot esculenta, Glycine max, Solanum lycopersicum,
  • the plant, plant protoplast or plant cell is Solanum tuberosum.
  • Another aspect of the invention relates to a method for improving drought resistance in plants, comprising producing a plurality of plants, plant protoplasts or plant cells that have been genetically engineered to overexpress or express de novo a KGDR06 KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, and testing the genetically-engineered plants, plant protoplasts or plant cells for improved drought resistance and selecting a plant, plant protoplast or plant cell having improved drought resistance, wherein the KGDR06 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53, wherein the KGDR26 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69,
  • a further aspect of the invention relates to a plant, plant protoplast or plant cell having improved drought resistance, wherein the plant, plant protoplast or plant cell has been genetically engineered to overexpress or express de novo a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, wherein the KGDR06 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53, wherein the KGDR26 protein comprises an amino acid sequence having at least 85% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89, wherein the KGDR25 protein
  • the plant, plant protoplast or plant cell is selected from the group consisting of Solanum tuberosum, Zea mays, Triticum spp. , Triticum aestivum, Oryza sativa, Sorghum bicolor, Dioscorea spp. , Musa spp. , Manihot esculenta, Glycine max, Solanum lycopersicum, Gossypium hirsutum, Hordeum vulgare, Avena sativa, Secale cereale, and Brassica napus.
  • the plant, plant protoplast or plant cell is Solanum tuberosum.
  • the KGDR06 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the KGDR06 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 28, 30, 32, 34, 36, 38, 44, 46, 48, 50, 52, and 54.
  • the KGDR06 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 28, 30, 32, 34, 36, 38, 44, 46, 48, 50, 52, and 54.
  • the KGDR26 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the KGDR26 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the KGDR26 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the KGDR25 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15 .
  • the KGDR25 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the KGDR25 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the KGDR42 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the KGDR42 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the KGDR42 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the KGDR37 protein comprises an amino acid sequence having at least 95% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the KGDR37 protein is encoded by a nucleotide sequence having at least 85% sequence identity with any of SEQ ID NOs: 127, 129, 131 , 133, 135, 137, 139, 141 , 143, 145, 147, 149, 155, 157, 163, 165, 167 and 169.
  • the KGDR37 protein is encoded by a nucleotide sequence having at least 95% sequence identity with any of SEQ ID NOs: 127, 129, 131 , 133, 135, 137, 139, 141 , 143, 145, 147, 149, 155, 157, 163, 165, 167 and 169.
  • aligning and “alignment” refer to the comparison of two or more nucleotide sequences based on the presence of short or long stretches of identical or similar nucleotides. Several methods for alignment of nucleotide sequences are known in the art, as will be further explained below.
  • “Expression of a gene” refers to the process wherein a DNA region, which is operably linked to appropriate regulatory regions, particularly a promoter, is transcribed into an RNA, which is biologically active, i.e. which is capable of being translated into a biologically active protein or peptide (or active peptide fragment).
  • “Ectopic expression” refers to expression in a tissue in which the gene is normally not expressed.
  • “Expression of a protein” is used herein interchangeably with the term expression of a gene. It refers to the process in which a DNA region, which is operably linked to appropriate regulatory regions, particularly a promoter, is transcribed into an mRNA and which is subsequently translated into a protein or peptide (or active peptide fragment).
  • the term "gene” means a DNA sequence comprising a region (transcribed region), which is transcribed into an RNA molecule (e.g., an mRNA) in a cell, operably linked to suitable regulatory regions (e.g., a promoter).
  • a gene may thus comprise several operably linked sequences, such as a promoter, a 5' leader sequence comprising, e.g., sequences involved in translation initiation, a (protein) coding region (cDNA or genomic DNA) and a 3' non-translated sequence comprising, e.g., transcription termination sequence sites.
  • cDNA means complementary DNA.
  • Complementary DNA is made by reverse transcribing RNA into a complementary DNA sequence.
  • cDNA sequences thus correspond to RNA sequences that are expressed from genes.
  • mRNA sequences when expressed from the genome can undergo splicing, i.e., introns are spliced out of the mRNA and exons are joined together, before being translated in the cytoplasm into proteins, it is understood that expression of a cDNA means expression of the mRNA that encodes for the cDNA.
  • the cDNA sequence thus may not be identical to the genomic DNA sequence to which it corresponds as cDNA may encode only the complete open reading frame, consisting of the joined exons, for a protein, whereas the genomic DNA encodes and exons interspersed by intron sequences. Genetically modifying a gene which encodes the cDNA may thus not only relate to modifying the sequences corresponding to the cDNA, but may also involve mutating intronic sequences of the genomic DNA and/or other gene regulatory sequences of that gene, as long as it results in the impairment of gene expression.
  • Identity is a measure of the identity of nucleotide sequences or amino acid sequences. In general, the sequences are aligned so that the highest order match is obtained. “Identity” per se has an art-recognized meaning and can be calculated using published techniques (see, e.g.,
  • identity is well known to skilled artisans (Carillo, H., and Lipton, D., SIAM J. Applied Math (1988) 48:1073). Methods commonly employed to determine identity or similarity between two sequences include, but are not limited to, those disclosed in GUIDE TO HUGE COMPUTERS, Martin J. Bishop, ed., Academic Press, San Diego, 1994, and Carillo, H., and Lipton, D., SIAM J. Applied Math (1988) 48:1073. Methods to determine identity and similarity are codified in computer programs.
  • Preferred computer program methods to determine identity and similarity between two sequences include, but are not limited to, GCS program package (Devereux, J., et al., Nucleic Acids Research (1984) 12(1 ):387), BLASTP, BLASTN, FASTA (Atschul, S. F. et al., J. Molec. Biol. (1990) 215:403).
  • nucleotide sequence having at least, for example, 95% "identity" to a reference nucleotide sequence encoding a polypeptide of a certain sequence it is intended that the nucleotide sequence of the polynucleotide is identical to the reference sequence except that the polynucleotide sequence may include up to five point mutations per each 100 nucleotides of the reference polypeptide sequence.
  • the percentage of identity of a nucleotide sequence to a reference nucleotide sequence is calculated over the entire length of the reference nucleotide sequence.
  • nucleotide having a nucleotide sequence at least 95% identical to a reference nucleotide sequence up to 5% of the nucleotides in the reference sequence may be deleted and/or substituted with another nucleotide, and/or a number of nucleotides up to 5% of the total nucleotides in the reference sequence may be inserted into the reference sequence.
  • mutations of the reference sequence may occur at the 5' or 3' terminal positions of the reference nucleotide sequence, or anywhere between those terminal positions, interspersed either individually among nucleotides in the reference sequence or in one or more contiguous groups within the reference sequence.
  • polypeptide having an amino acid sequence having at least, for example, 95% "identity" to a reference amino acid sequence is intended that the amino acid sequence of the polypeptide is identical to the reference sequence except that the polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the reference amino acid. Hence, the percentage of identity of an amino acid sequence to a reference amino acid sequence is calculated over the entire length of the reference amino acid sequence.
  • a polypeptide having an amino acid sequence at least 95% identical to a reference amino acid sequence up to 5% of the amino acid residues in the reference sequence may be deleted or substituted with another amino acid, or a number of amino acids up to 5% of the total amino acid residues in the reference sequence may be inserted into the reference sequence.
  • These alterations of the reference sequence may occur at the amino or carboxy terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence or in one or more contiguous groups within the reference sequence.
  • a nucleic acid according to the present invention may include any polymer or oligomer of pyrimidine and purine bases, preferably cytosine, thymine, and uracil, and adenine and guanine, respectively (See Albert L. Lehninger, Principles of Biochemistry, at 793-800 (Worth Pub. 1982) which is herein incorporated by reference in its entirety for all purposes).
  • the present invention contemplates any deoxyribonucleotide, ribonucleotide or peptide nucleic acid component, and any chemical variants thereof, such as methylated, hydroxymethylated or glycosylated forms of these bases, and the like.
  • the polymers or oligomers may be heterogenous or homogenous in composition, and may be isolated from naturally occurring sources or may be artificially or synthetically produced.
  • the nucleic acids may be DNA or RNA, or a mixture thereof, and may exist permanently or transitionally in single- stranded or double-stranded form, including homoduplex, heteroduplex, and hybrid states.
  • KGDR06 proteins refer to a group of plant proteins characterized by their sequence homology to the protein encoded by Arabidopsis At5G64660 gene (e.g., having at least 30%, or at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or at least 99% sequence identity to the amino acid sequence of At5G64660), as well as their functionality in improving drought resistance when overexpressed or expressed de novo in a plant.
  • the KGDR06 proteins include at least At5G64660 (SEQ ID NO:1 ) and its homologs from corn (GRMZM2G303964_P01— SEQ ID NO:3; GRMZM2G127690_P01— SEQ ID NO:5;
  • GRMZM2G059042_P01 SEQ ID NO:7; GRMZM2G073310_P01— SEQ ID NO:9), wheat
  • LOC_Os04g34140.1 SEQ ID NO:15
  • LOC_Os05g 36360.1 SEQ ID NO:17
  • GLYMA05G32310.1 SEQ ID NO:45; GLYMA06G 15630.1— SEQ ID NO:47; GLYMA09G03520.2— SEQ ID NO:49; GLYMA16G28630.1— SEQ ID NO:51 ; GLYMA02G09240.1— SEQ ID NO:53).
  • KGDR06 genes refers genes encoding the KGDR06 proteins.
  • the KGDR06 genes include at least At5G64660 (SEQ ID NO:2) and its homologs from corn (GRMZM2G303964_T01 CDS— SEQ ID NO:4; GRMZM2G127690_T01 CDS— SEQ ID NO:6; GRMZM2G059042_T01 CDS— SEQ ID NO:8; GRMZM2G073310_T01 CDS— SEQ ID NO: 10), wheat (Traes_2BL_9FD31 E1 13.1 CDS —SEQ ID NO: 12), rice (LOC_Os02g33680.1 CDS— SEQ ID NO:14; LOC_Os04g34140.1 CDS— SEQ ID NO:16; LOC_Os05g36360.1 CDS— SEQ ID NO:18),sorghum (Sobic.004G169900.1 CDS— SEQ ID NO:20; Sobic.006
  • KGDR26 proteins refer to a group of plant proteins characterized by their sequence homology to the protein encoded by Arabidopsis At5G17880 gene (e.g., having at least 30%, or at least 40%, at least 50%, at least 60%, at least 70%, or at least 80% sequence identity to the amino acid sequence of At5G 17880), as well as their functionality in improving drought resistance when overexpressed or expressed de novo in a plant.
  • the KGDR26 proteins include at least At5G17880 (SEQ ID NO:55) and its homologs from corn (GRMZM2G394261_T01— SEQ ID NO:57), wheat (Traes_2AS_5A3373A3D.1— SEQ ID NO:59), sorghum (Sobic.002G342200.1— SEQ ID NO:61 ), potato (PGSC0003DMT400031455— SEQ ID NO:63; PGSC0003DMT400040535— SEQ ID NO:65; PGSC0003DMT400040531— SEQ ID NO:67; PGSC0003DMT400043748—SEQ ID NO:69;
  • PGSC0003DMT400006233 SEQ ID NO:71 ;
  • PGSC0003DMT400077128 SEQ ID NO:73;
  • PGSC0003DMT400006231 SEQ ID NO:75
  • PGSC0003DMT400043733 SEQ ID NO:77
  • PGSC0003DMT400040528 SEQ ID NO:79
  • PGSC0003DMT400043300 SEQ ID NO:81 ;
  • PGSC0003DMT400043719 SEQ ID NO:83
  • soybean GLYMA16G27521 .1— SEQ ID NO:85
  • GLYMA16G27544.1 SEQ ID NO:87
  • GLYMA16G27550.1 SEQ ID NO:89
  • KGDR26 genes refers genes encoding the KGDR26 proteins.
  • the KGDR26 genes include at least AT5G17880 CDS (SEQ ID NO:56) and its homologs from corn
  • Glyma.16G159500.1 CDS SEQ ID NO:88; Glyma.16G159600.1 CDS— SEQ ID NO:90).
  • KGDR25 proteins refer to a group of plant proteins characterized by their sequence homology to the protein encoded by Arabidopsis At5G16140 gene (e.g., having at least 30%, or at least 40%, at least 50%, at least 60%, at least 70%, or at least 80% sequence identity to the amino acid sequence of At5G16140), as well as their functionality in improving drought resistance when overexpressed or expressed de novo in a plant.
  • the KGDR25 proteins include at least At5G16140 (SEQ ID NO:91 ) and its homologs from corn (GRMZM2G132021_P01— SEQ ID NO:93;
  • GRMZM2G132021_P02 SEQ ID NO:95; GRMZM2G132021_P03— SEQ ID NO:97), wheat (TRAES3BF010400420CFD_t1— SEQ ID NO:99; Traes_3AS_7BF10F95B.2— SEQ ID NO:100; Traes_3DS_79443B666.2_P01— SEQ ID NO: 102), sorghum (Sb03g007130.1— SEQ ID NO: 104), yam
  • KGDR25 genes refers genes encoding the KGDR25 proteins.
  • the KGDR25 genes include at least At5G16140 (SEQ ID NO:92) and its homologs from corn (GRMZM2G132021_T01— SEQ ID NO:94; GRMZM2G132021_T02— SEQ ID NO:96; GRMZM2G132021_T03— SEQ ID NO:98), wheat (Traes_3AS_7BF10F95B.2— SEQ ID NO:101 ; Traes_3DS_79443B666.2_P01— SEQ ID NO: 103), sorghum (Sobic.003G084500.3— SEQ ID NO: 105), plantain (GSMUA_Achr5P23710_001— SEQ ID NO: 108), potato (PGSC0003DMT400067854— SEQ ID NO: 1 10), and soybean
  • KGDR42 proteins refer to a group of plant proteins characterized by their sequence homology to the protein encoded by Arabidopsis At2G07768 gene (e.g., having at least 30%, or at least 40%, at least 50%, at least 60%, at least 70%, or at least 80% sequence identity to the amino acid sequence of At2G07768), as well as their functionality in improving drought resistance when overexpressed or expressed de novo in a plant.
  • the KGDR42 proteins include at least At2G07768 (SEQ ID NO: 1 17) and its homologs from corn (GRMZM5G867512_P01— SEQ ID NO: 1 19;
  • GRMZM2G314328_P01 SEQ ID NO: 121
  • wheat Traes_2AL_9FBD6EB21 .1— SEQ ID NO: 123
  • yam sending let 1 18932— SEQ ID NO: 125.
  • KGDR42 genes refers genes encoding the KGDR42 proteins.
  • the KGDR42 genes include at least AT2G07768 CDS (SEQ ID NO:1 18) and its homologs from corn
  • KGDR37 proteins refer to a group of plant proteins characterized by their sequence homology to the protein encoded by Arabidopsis At1 G49300 gene (e.g., having at least 30%, or at least 40%, at least 50%, at least 60%, at least 70%, or at least 80% sequence identity to the amino acid sequence of At1 G49300), as well as their functionality in improving drought resistance when overexpressed or expressed de novo in a plant.
  • the KGDR37 proteins include at least At1 G49300
  • GRMZM5G81 1797_T05 SEQ ID NO: 130; GRMZM2G158887_T01— SEQ ID NO: 132), wheat
  • PGSC0003DMT400006517 SEQ ID NO: 156
  • cassava cassava6978.valid.ini— SEQ ID NO: 158
  • cassava19087.valid.ini SEQ ID NO: 159
  • cassava31439.valid.m1 SEQ ID NO: 160;
  • KGDR37 genes refers genes encoding the KGDR37 proteins.
  • the KGDR37 genes include at least At1 G49300 (SEQ ID NO: 127) and its homologs from corn (GRMZM2G044368_T01 CDS— SEQ ID NO: 129; GRMZM5G81 1797_T05 CDS— SEQ ID NO: 131 ; GRMZM2G158887_T01 CDS—SEQ ID NO: 133), wheat (Traes_1AL_E07235CF1.1 CDS—SEQ ID NO: 135; Traes_1 DL_4A9307E37.1 CDS— SEQ ID NO: 137; Traes_1 BL_212EC3A2C1 CDS— SEQ ID NO: 139; Traes_3AL_078057859.1 CDS—SEQ ID N0:141 ; Traes_3AS_B8C00449D.1 CDS—SEQ ID NO: 143; Traes_3
  • the term "functional" in relation to any one of KGDR06, KGDR26, KGDR25, KGDR42 and KGDR37 proteins or genes refers to the capability of the gene and/or encoded protein to modify the (quantitative and/or qualitative) drought resistance, e.g., by modifying the expression level of the gene (e.g., by overexpression or de novo expression) in a plant.
  • the functionality of a KGDR06 protein obtained from plant species X can be tested by various methods.
  • the protein is a functional KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein
  • overexpressing the gene encoding the protein in plant species X or expressing de novo the gene encoding the protein in a different plant species can lead to improved drought resistance as can be tested as explained herein in detail.
  • silencing the overexpressed or de novo expressed KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein can restore the drought sensitivity of the wild-type plants. The skilled person will be able to test such functionality.
  • a "vector,” “expression vector” or “expression construct” refers to a recombinant nucleic acid molecule which is used to deliver exogenous DNA into a host cell.
  • the vector backbone may for example be a binary or superbinary vector (see e.g. U.S. Pat. No. 5,591 ,616, US 2002138879 and WO 95/06722), a co-integrate vector or a T-DNA vector, as known in the art and as described elsewhere herein, into which a chimeric gene is integrated or, if a suitable transcription regulatory sequence is already present, only a desired nucleotide sequence (e.g.
  • Vectors usually comprise further genetic elements to facilitate their use in molecular cloning, such as e.g. selectable markers, multiple cloning sites and the like.
  • operably linked refers to a linkage of polynucleotide elements in a functional relationship.
  • a nucleic acid is “operably linked” when it is placed into a functional relationship with another nucleotide sequence.
  • a promoter or rather a transcription regulatory sequence, is operably linked to a coding sequence if it affects the transcription of the coding sequence.
  • Operably linked may mean that the DNA sequences being linked are contiguous.
  • Plant refers to either the whole plant or to parts of a plant, such as cells, tissue or organs (e.g. pollen, seeds, gametes, roots, leaves, flowers, flower buds, anthers, fruit, etc.) obtainable from the plant, as well as derivatives of any of these and progenies derived from such a plant by selfing or crossing.
  • Plant cell(s) include protoplasts, gametes, suspension cultures, microspores, pollen grains, etc., either in isolation or within a tissue, organ or organism.
  • Promoter refers to a nucleic acid fragment that functions to control the transcription of one or more genes, located upstream with respect to the direction of transcription of the transcription initiation site of the gene, and is structurally identified by the presence of a binding site for DNA- dependent RNA polymerase, transcription initiation sites and any other DNA sequences, including, but not limited to transcription factor binding sites, repressor and activator protein binding sites, and any other sequences of nucleotides known to one of skill in the art to act directly or indirectly to regulate the amount of transcription from the promoter.
  • promoter includes also the 5' UTR region (5' Untranslated Region) (e.g.
  • the promoter may herein include one or more parts upstream of the translation initiation codon of a gene, as this region may have a role in regulating transcription and/or translation).
  • a "constitutive" promoter is a promoter that is active in most tissues under most physiological and developmental conditions.
  • An “inducible” promoter is a promoter that is
  • tissue specific promoter is only active in specific types of tissues or cells.
  • promoter active in plants or plant cells refers to the general capability of the promoter to drive transcription within a plant or plant cell. It does not make any implications about the spatio-temporal activity of the promoter.
  • protein or “polypeptide” are used interchangeably and refer to molecules consisting of a chain of amino acids, without reference to a specific mode of action, size, 3 dimensional structure or origin. A “fragment” or “portion” of a protein may thus still be referred to as a "protein.”
  • isolated protein is used to refer to a protein which is no longer in its natural environment, for example in vitro or in a recombinant bacterial or plant host cell.
  • Genetically-engineered plant or “transformed plant” refers herein to a plant or plant cell that has been genetically engineered to introduce for example one or more insertions of a gene expression construct in the genome.
  • a genetically-engineered plant cell may refer to a plant cell in isolation or in tissue culture, or to a plant cell contained in a plant or in a differentiated organ or tissue, and both possibilities are specifically included herein.
  • a reference to a plant cell in the description or claims is not meant to refer only to isolated cells or protoplasts in culture, but refers to any plant cell, wherever it may be located or in whatever type of plant tissue or organ it may be present.
  • the term "drought stress” or “drought” refers to a sub-optimal environmental condition associated with limited availability of water to a plant. Limited availability of water may occur when for instance rain is absent or lower and/or when the plants are watered less frequently than required. Limited water availability to a plant may also occur when for instance water is present in soil, but cannot efficiently be extracted by the plant. For instance, when soils strongly bind water or when the water has a high salt content, it may be more difficult for a plant to extract the water from the soil. Hence, many factors can contribute to result in limited availability of water, i.e. drought, to a plant.
  • the effect of subjecting plants to "drought” or “drought stress” may be that plants do not have optimal growth and/or development.
  • Plants subjected to drought may have wilting signs.
  • plants may be subjected to a period of at least 15 days under specific controlled conditions wherein no water is provided, e.g. without rain fall and/or watering of the plants.
  • improved drought resistance refers to plants which, when provided with improved drought resistance, when subjected to drought or drought stress do not show effects or show alleviated effects as observed in plants not provided with improved drought resistance.
  • a normal plant has some level of drought resistance. It can easily be determined whether a plant has improved drought resistant by comparing a control plant with a plant provided with improved drought resistance under controlled conditions chosen such that in the control plants signs of drought can be observed after a certain period, i.e. when the plants are subjected to drought or drought stress.
  • the plants with improved drought resistance will show less and/or reduced signs of having been subjected to drought, such as wilting, as compared to the control plants.
  • a control plant, plant protoplast or plant cell is preferably a plant, plant protoplast or plant cell that differs from the plant, plant protoplast or plant cell having improved drought resistance in that the control does not express de novo or does not overexpress the drought resistance protein as defined herein.
  • the only difference between the control and the plant (plant protoplast or plant cell) having an improved drought resistance is that the control does not express de novo or does not overexpress the drought resistance protein as defined herein.
  • the control may be a wildtype plant, plant protoplast or plant cell or the control may comprise an empty vector.
  • the empty vector comprises the same backbone as the vector used for overexpression or de novo expression of the functional drought resistance protein as defined herein.
  • One aspect of the invention described herein relates to a method for improving drought resistance in plants, comprising overexpressing or expressing de novo a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, in a plant, plant protoplast or plant cell, wherein the KGDR06 protein comprises an amino acid seguence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% seguence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53, wherein the KGDR26 protein comprises an amino acid seguence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% seguence identity with any of SEQ ID NOs: 55,
  • KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein has an amino acid sequence as it occurs in nature, e.g. as it can be isolated from a wild type plant.
  • Preferred wild type plants are e.g. Arabidopsis, corn, wheat, rice, sorghum, yam, potato, plantain, potato, cassava or soybean.
  • amino acid sequence can encode engineered forms of any of the KGDR06 KGDR26, KGDR25, KGDR42 or KGDR37 proteins as defined herein, wherein the amino acid sequence comprises one or more amino acid substitutions, insertions and/or deletions as compared to the corresponding naturally occurring KGDR06 protein but that are within the ranges of identity as defined herein.
  • the KGDR06 protein can be encoded by, for example, a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 28, 30, 32, 34, 36, 38, 44, 46, 48, 50, 52, and 54.
  • the KGDR26 protein can be encoded by, for example, a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the KGDR25 protein can be encoded by, for example, a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the KGDR42 protein can be encoded by, for example, a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the KGDR37 protein can be encoded by, for example, a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 127, 129, 131 , 133, 135, 137, 139, 141 , 143, 145, 147, 149, 155, 157, 163, 165, 167 and 169.
  • the method can comprise, for example, genetically engineering the plant, plant protoplast or plant cell to overexpress or express de novo the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • Overexpression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof can be achieved by, for example, inserting at least one additional copy of an endogenous gene encoding the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, into the plant, plant protoplast or plant cell.
  • Further ways are modulating promoter and/or further regulating sequences that are operably linked to the KGDR06-, KGDR26-, KGDR25-, KGDR42- or KGDR37-encoding sequence and resulting in enhanced expression.
  • the method can comprise, for example, transforming a plant protoplast or plant cell with a vector or expression construct comprising a recombinant nucleic acid encoding the KGDR06 protein.
  • the method can comprise, for example, Agrobacterium-mediated transformation (e.g., contacting the plant protoplast or plant cell with an Agrobacterium strain comprising the vector or expression construct to introduce the recombinant nucleic acid into the plant protoplast or plant cell).
  • Agrobacterium-mediated transformation e.g., contacting the plant protoplast or plant cell with an Agrobacterium strain comprising the vector or expression construct to introduce the recombinant nucleic acid into the plant protoplast or plant cell.
  • the method can further comprise, for example, regenerating the plant protoplast or plant cell into a plant.
  • the method can further comprise, for example, producing seeds from the plant having improved drought resistance.
  • the method can further comprise, for example, growing the seeds into plants having improved drought resistance.
  • the method can further comprise, for example, testing the plant, plant protoplast or plant cell for overexpression or de novo expression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • Methods for testing overexpression or de novo expression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof include, but are not limited to, PCR analysis, sequencing of genomic DNA, sequencing of mRNA transcript, analyzing mRNA transcript levels (Northern-blot analysis), analyzing copy number (Southern blot analysis), etc.
  • the method can further comprise, for example, testing the plant, plant protoplast or plant cell for improved drought resistance.
  • Methods for testing drought resistance include, but are not limited to, the drought assay described in WO 2013/122471 , WO 2013/122472 and WO 2013/122473, which are incorporated herein by reference in their entireties, as well as field tests under water-stress conditions as described in the working examples of the present application.
  • the method can further comprise, for example, producing progenies of the plant, plant protoplast or plant cell and selecting one or more progenies that overexpress or express de novo the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • the method can further comprise, for example, producing progenies of the plant, plant protoplast or plant cell and selecting one or more progenies plants that have improved drought resistance.
  • the plant, plant protoplast or plant cell can be selected from, for example, Solanum tuberosum, Zea mays, Triticum spp. , Triticum aestivum, Oryza sativa, Sorghum bicolor, Dioscorea spp. , Musa spp. , Manihot esculenta, Glycine max, Solanum lycopersicum, Gossypium hirsutum, Hordeum vulgare, Avena sativa, Secale cereale, and Brassica napus.
  • the plant, plant protoplast or plant cell is Solanum tuberosum.
  • the method can comprise, for example, overexpressing in a corn plant (e.g., Zea mays) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 3, 5, 7, and 9) or expressing de novo in a corn plant an homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 3, 5, 7, and 9.
  • a corn plant e.g., Zea mays
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 3, 5, 7, and 9
  • an homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 3, 5, 7, and 9.
  • the method can comprise, for example, expressing de novo in a corn plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39- 43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a wheat plant (e.g., Triticum aestivum) an endogenous KGDR06 protein (e.g., SEQ ID NO: 1 1 ) or expressing de novo in a wheat plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 1 1.
  • a wheat plant e.g., Triticum aestivum
  • an endogenous KGDR06 protein e.g., SEQ ID NO: 1 1
  • expressing de novo in a wheat plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 1 1.
  • the method can comprise, for example, expressing de novo in a wheat plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a rice plant (e.g., Oryza sativa) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 13, 15, and 17) or expressing de novo in a sorghum plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 13, 15, and 17.
  • a rice plant e.g., Oryza sativa
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 13, 15, and 17
  • a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 13, 15, and 17.
  • the method can comprise, for example, expressing de novo in a rice plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39- 43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a sorghum plant (e.g., Sorghum bicolor) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 19, 21 , and 23) or expressing de novo in a sorghum plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 19, 21 , and 23.
  • a sorghum plant e.g., Sorghum bicolor
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 19, 21 , and 23
  • expressing de novo in a sorghum plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 19, 21 , and
  • the method can comprise, for example, expressing de novo in a sorghum plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a yam plant (e.g., Dioscorea spp.) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 25 and/or 26) or expressing de novo in a yam plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 25 and/or 26.
  • a yam plant e.g., Dioscorea spp.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 25 and/or 26
  • expressing de novo in a yam plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 25 and/or
  • the method can comprise, for example, expressing de novo in a yam plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a plantain/banana plant (e.g., Musa spp.) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 27, 29, 31 , and 33) or expressing de novo in a plantain plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 27, 29, 31 , and 33.
  • a plantain/banana plant e.g., Musa spp.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 27, 29, 31 , and 33
  • expressing de novo in a plantain plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ
  • the method can comprise, for example, expressing de novo in a plantain plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25, 26, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a potato plant (e.g., Solanum tuberosum) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 35 and/or 37) or expressing de novo in a potato plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 35 and/or 37.
  • a potato plant e.g., Solanum tuberosum
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 35 and/or 37
  • expressing de novo in a potato plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 35 and/or 37.
  • the method can comprise, for example, expressing de novo in a potato plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a cassava plant (e.g., Manihot esculenta) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 39, 40, 41 , and 42) or expressing de novo in a cassava plant a homologous KGDR06 protein having at least 70%, at least 80%, at least
  • the method can comprise, for example, expressing de novo in a cassava plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, overexpressing in a soybean plant (e.g., Glycine max) an endogenous KGDR06 protein (e.g., SEQ ID NOs: 43, 45, 47, 49, 51 , and 53) or expressing de novo in a soybean plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 43, 45, 47, 49, 51 , and 53.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 43, 45, 47, 49, 51 , and 53
  • expressing de novo in a soybean plant a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, expressing de novo in a soybean plant a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, and 39-42.
  • the method can comprise, for example, expressing de novo in a tomato plant (e.g., Solanum lycopersicum) a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least
  • the method can comprise, for example, expressing de novo in a cotton plant (e.g., Gossypium hirsutum) a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a cotton plant e.g., Gossypium hirsutum
  • KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47
  • the method can comprise, for example, expressing de novo in a barley plant (e.g., Hordeum vulgare) a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a barley plant e.g., Hordeum vulgare
  • KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, expressing de novo in an oat plant (e.g., Avena sativa) a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • an oat plant e.g., Avena sativa
  • KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53
  • the method can comprise, for example, expressing de novo in a rye plant (e.g., Secale cereale) a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a rye plant e.g., Secale cereale
  • KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the method can comprise, for example, expressing de novo in a rapeseed plant (e.g., Brassica napus) a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a rapeseed plant e.g., Brassica napus
  • KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53
  • the method can comprise, for example, overexpressing in a corn plant (e.g., Zea mays) an endogenous KGDR26 protein (e.g., SEQ ID NO: 57) or expressing de novo in a corn plant an homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 57.
  • a corn plant e.g., Zea mays
  • an endogenous KGDR26 protein e.g., SEQ ID NO: 57
  • SEQ ID NO: 57 an endogenous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 57.
  • the method can comprise, for example, expressing de novo in a corn plant a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87, and 89.
  • the method can comprise, for example, overexpressing in a wheat plant (e.g., Triticum aestivum) an endogenous KGDR26 protein (e.g., SEQ ID NO: 59) or expressing de novo in a wheat plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 59.
  • a wheat plant e.g., Triticum aestivum
  • an endogenous KGDR26 protein e.g., SEQ ID NO: 59
  • expressing de novo in a wheat plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 59.
  • the method can comprise, for example, expressing de novo in a wheat plant a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87, and 89.
  • the method can comprise, for example, overexpressing in a sorghum plant (e.g., Sorghum bicolor) an endogenous KGDR26 protein (e.g., SEQ ID NO: 61 ) or expressing de novo in a sorghum plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 61.
  • a sorghum plant e.g., Sorghum bicolor
  • an endogenous KGDR26 protein e.g., SEQ ID NO: 61
  • expressing de novo in a sorghum plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 61.
  • the method can comprise, for example, expressing de novo in a sorghum plant a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87, and 89.
  • the method can comprise, for example, overexpressing in a potato plant (e.g., Solanum tuberosum) an endogenous KGDR26 protein (e.g., SEQ ID NOs: 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83) or expressing de novo in a potato plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83.
  • an endogenous KGDR26 protein e.g., SEQ ID NOs: 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83
  • the method can comprise, for example, expressing de novo in a potato plant a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 85, 87, and 89.
  • the method can comprise, for example, overexpressing in a soybean plant (e.g., Glycine max) an endogenous KGDR26 protein (e.g., SEQ ID NOs: 85, 87, and 89) or expressing de novo in a soybean plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 85, 87, and 89.
  • a soybean plant e.g., Glycine max
  • an endogenous KGDR26 protein e.g., SEQ ID NOs: 85, 87, and 89
  • expressing de novo in a soybean plant a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 85, 87, and 89.
  • the method can comprise, for example, expressing de novo in a soybean plant a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83.
  • the method can comprise, for example, expressing de novo in a rice plant (e.g., Oryza sativa) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a rice plant e.g., Oryza sativa
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the method can comprise, for example, expressing de novo in a yam plant (e.g.,
  • Dioscorea spp. a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the method can comprise, for example, expressing de novo in a plantain or banana plant (e.g., Musa spp.) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a plantain or banana plant e.g., Musa spp.
  • a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79,
  • the method can comprise, for example, expressing de novo in a cassava plant (e.g., Manihot esculenta) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a cassava plant e.g., Manihot esculenta
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 ,
  • the method can comprise, for example, expressing de novo in a tomato plant (e.g., Solanum lycopersicum) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a tomato plant e.g., Solanum lycopersicum
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 ,
  • the method can comprise, for example, expressing de novo in a cotton plant (e.g.,
  • Gossypium hirsutum) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the method can comprise, for example, expressing de novo in a barley plant (e.g., Hordeum vulgare) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a barley plant e.g., Hordeum vulgare
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85,
  • the method can comprise, for example, expressing de novo in an oat plant (e.g., Avena sativa) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • an oat plant e.g., Avena sativa
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83
  • the method can comprise, for example, expressing de novo in a rye plant (e.g., Secale cereale) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a rye plant e.g., Secale cereale
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85
  • the method can comprise, for example, expressing de novo in a rapeseed plant (e.g., Brassica napus) a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a rapeseed plant e.g., Brassica napus
  • KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83
  • the method can comprise, for example, overexpressing in a corn plant (e.g., Zea mays) an endogenous KGDR25 protein (e.g., SEQ ID NOs: 93, 95, and 97) or expressing de novo in a corn plant an homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 93, 95, and 97.
  • a corn plant e.g., Zea mays
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 93, 95, and 97
  • an homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 93, 95, and 97.
  • the method can comprise, for example, expressing de novo in a corn plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a wheat plant (e.g., Triticum aestivum) an endogenous KGDR25 protein (e.g., SEQ ID NOs: 99, 100, and 102) or expressing de novo in a wheat plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 99, 100, and 102.
  • a wheat plant e.g., Triticum aestivum
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 99, 100, and 102
  • a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 99, 100, and 102.
  • the method can comprise, for example, expressing de novo in a wheat plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a sorghum plant (e.g., Sorghum bicolor) an endogenous KGDR25 protein (e.g., SEQ ID NO: 104) or expressing de novo in a sorghum plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 104.
  • a sorghum plant e.g., Sorghum bicolor
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 104
  • a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 104.
  • the method can comprise, for example, expressing de novo in a sorghum plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a yam plant (e.g., Dioscorea spp.) an endogenous KGDR25 protein (e.g., SEQ ID NO: 106) or expressing de novo in a yam plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 106.
  • a yam plant e.g., Dioscorea spp.
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 106
  • the method can comprise, for example, expressing de novo in a yam plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a plantain plant (e.g., Musa spp.) an endogenous KGDR25 protein (e.g., SEQ ID NO: 107) or expressing de novo in a plantain plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 107.
  • a plantain plant e.g., Musa spp.
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 107
  • the method can comprise, for example, expressing de novo in a plantain plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a potato plant (e.g., Solanum tuberosum) an endogenous KGDR25 protein (e.g., SEQ ID NO: 109) or expressing de novo in a potato plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 109.
  • a potato plant e.g., Solanum tuberosum
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 109
  • the method can comprise, for example, expressing de novo in a potato plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a cassava plant (e.g., Manihot esculenta) an endogenous KGDR25 protein (e.g., SEQ ID NOs: 1 1 1 and 1 12) or expressing de novo in a cassava plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 1 1 and 1 12.
  • a cassava plant e.g., Manihot esculenta
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 1 1 1 and 1 12
  • expressing de novo in a cassava plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 1 1 and 1 12.
  • the method can comprise, for example, expressing de novo in a cassava plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a soybean plant (e.g., Glycine max) an endogenous KGDR25 protein (e.g., SEQ ID NOs: 1 13 and 1 15) or expressing de novo in a soybean plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 13 and 1 15.
  • a soybean plant e.g., Glycine max
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 1 13 and 1 15
  • expressing de novo in a soybean plant a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in a soybean plant a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 1 1 and 1 12.
  • the method can comprise, for example, expressing de novo in a rice plant (e.g., Oryza sativa) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • a rice plant e.g., Oryza sativa
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in a tomato plant (e.g., Solanum lycopersicum) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • a tomato plant e.g., Solanum lycopersicum
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in a cotton plant (e.g., Gossypium hirsutum) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • a cotton plant e.g., Gossypium hirsutum
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in a barley plant (e.g., Hordeum vulgare) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • a barley plant e.g., Hordeum vulgare
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in an oat plant (e.g., Avena sativa) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • an oat plant e.g., Avena sativa
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in a rye plant (e.g., Secale cereale) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • a rye plant e.g., Secale cereale
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, expressing de novo in a rapeseed plant (e.g., Brassica napus) a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • a rapeseed plant e.g., Brassica napus
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99, 100, 102, 104, 106, 107, 109, 1 10-1 13 and 1 15.
  • the method can comprise, for example, overexpressing in a corn plant (e.g., Zea mays) an endogenous KGDR42 protein (e.g., SEQ ID NOs: 1 19 and 121 ) or expressing de novo in a corn plant an homologous KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 19 and 121.
  • a corn plant e.g., Zea mays
  • an endogenous KGDR42 protein e.g., SEQ ID NOs: 1 19 and 121
  • an homologous KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 19 and 121.
  • the method can comprise, for example, expressing de novo in a corn plant a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 123, and 125.
  • the method can comprise, for example, overexpressing in a wheat plant (e.g., Triticum aestivum) an endogenous KGDR42 protein (e.g., SEQ ID NO: 123) or expressing de novo in a wheat plant a homologous KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 123.
  • a wheat plant e.g., Triticum aestivum
  • an endogenous KGDR42 protein e.g., SEQ ID NO: 123
  • the method can comprise, for example, expressing de novo in a wheat plant a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , and 125.
  • the method can comprise, for example, overexpressing in a yam plant (e.g., Dioscorea spp.) an endogenous KGDR42 protein (e.g., SEQ ID NO: 125) or expressing de novo in a yam plant a homologous KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 125.
  • a yam plant e.g., Dioscorea spp.
  • an endogenous KGDR42 protein e.g., SEQ ID NO: 125
  • a homologous KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 125.
  • the method can comprise, for example, expressing de novo in a corn plant a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , and 123.
  • the method can comprise, for example, expressing de novo in a rice plant (e.g., Oryza sativa) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a rice plant e.g., Oryza sativa
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a sorghum plant (e.g., Sorghum bicolor) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a sorghum plant e.g., Sorghum bicolor
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a plantain or banana plant (e.g., Musa spp.) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a plantain or banana plant e.g., Musa spp.
  • a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a potato plant (e.g., Solanum tuberosum) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a potato plant e.g., Solanum tuberosum
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a cassava plant (e.g., Manihot esculenta) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a cassava plant e.g., Manihot esculenta
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a soybean plant (e.g., Glycine max) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a soybean plant e.g., Glycine max
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a tomato plant (e.g., Solanum lycopersicum) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a tomato plant e.g., Solanum lycopersicum
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a cotton plant (e.g.,
  • Gossypium hirsutum a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a barley plant (e.g., Hordeum vulgare) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a barley plant e.g., Hordeum vulgare
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in an oat plant (e.g., Avena sativa) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • an oat plant e.g., Avena sativa
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a rye plant (e.g., Secale cereale) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a rye plant e.g., Secale cereale
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, expressing de novo in a rapeseed plant (e.g., Brassica napus) a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a rapeseed plant e.g., Brassica napus
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the method can comprise, for example, overexpressing in a corn plant (e.g., Zea mays) an endogenous KGDR37 protein (e.g., SEQ ID NOs: 128, 130,and 132) or expressing de novo in a corn plant an homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 128, 130,and 132.
  • a corn plant e.g., Zea mays
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 128, 130,and 132
  • an homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 128, 130,and 132.
  • the method can comprise, for example, expressing de novo in a corn plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a wheat plant (e.g., Triticum aestivum) an endogenous KGDR37 protein (e.g., SEQ ID NOs: 134, 136, 138, 140, 142, and 144) or expressing de novo in a wheat plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 134, 136, 138, 140, 142, and 144.
  • a wheat plant e.g., Triticum aestivum
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 134, 136, 138, 140, 142, and 144
  • SEQ ID NOs: 134, 136, 138, 140, 142, and 144 e.g., SEQ ID NOs: 134, 136
  • the method can comprise, for example, expressing de novo in a wheat plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a rice plant (e.g., Oryza sativa) an endogenous KGDR37 protein (e.g., SEQ ID NO: 146) or expressing de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 146.
  • a rice plant e.g., Oryza sativa
  • an endogenous KGDR37 protein e.g., SEQ ID NO: 1466
  • de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 146.
  • the method can comprise, for example, expressing de novo in a rice plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a sorghum plant (e.g., Sorghum bicolor) an endogenous KGDR37 protein (e.g., SEQ ID NO: 148) or expressing de novo in a sorghum plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 148.
  • a sorghum plant e.g., Sorghum bicolor
  • an endogenous KGDR37 protein e.g., SEQ ID NO: 1408
  • the method can comprise, for example, expressing de novo in a sorghum plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 150-154, 156, 158- 162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a yam plant (e.g., Dioscorea spp.) an endogenous KGDR37 protein (e.g., SEQ ID NOs: 150, 151 , 152 and 153) or expressing de novo in a yam plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 150, 151 , 152 and 153.
  • a yam plant e.g., Dioscorea spp.
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 150, 151 , 152 and 153
  • a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs
  • the method can comprise, for example, expressing de novo in a yam plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 154, 156, 158-162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a potato plant (e.g., Solanum tuberosum) an endogenous KGDR37 protein (e.g., SEQ ID NOs: 154 and 156) or expressing de novo in a potato plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 154 and 156.
  • a potato plant e.g., Solanum tuberosum
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 154 and 156
  • expressing de novo in a potato plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 154 and 156.
  • the method can comprise, for example, expressing de novo in a potato plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-153, 158-162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a cassava plant (e.g., Manihot esculenta) an endogenous KGDR37 protein (e.g., SEQ ID NOs: 158-161 ) or expressing de novo in a cassava plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 158-161.
  • a cassava plant e.g., Manihot esculenta
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 158-161
  • expressing de novo in a cassava plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 158-161.
  • the method can comprise, for example, expressing de novo in a cassava plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 162, 164, 166 and 168.
  • the method can comprise, for example, overexpressing in a soybean plant (e.g., Glycine max) an endogenous KGDR37 protein (e.g., SEQ ID NOs: 162, 164, 166 and 168) or expressing de novo in a soybean plant a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 162, 164, 166 and 168.
  • a soybean plant e.g., Glycine max
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 162, 164, 166 and 168
  • the method can comprise, for example, expressing de novo in a soybean plant a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-161.
  • the method can comprise, for example, expressing de novo in a plantain or banana plant (e.g., Musa spp.) a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a plantain or banana plant e.g., Musa spp.
  • a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154,
  • the method can comprise, for example, expressing de novo in a tomato plant (e.g.,
  • Solanum lycopersicum a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the method can comprise, for example, expressing de novo in a cotton plant (e.g., Gossypium hirsutum) a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a cotton plant e.g., Gossypium hirsutum
  • KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154
  • the method can comprise, for example, expressing de novo in a barley plant (e.g., Hordeum vulgare) a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a barley plant e.g., Hordeum vulgare
  • KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164,
  • the method can comprise, for example, expressing de novo in an oat plant (e.g., Avena sativa) a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • an oat plant e.g., Avena sativa
  • KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162,
  • the method can comprise, for example, expressing de novo in a rye plant (e.g., Secale cereale) a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a rye plant e.g., Secale cereale
  • KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164
  • the method can comprise, for example, expressing de novo in a rapeseed plant (e.g., Brassica napus) a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a rapeseed plant e.g., Brassica napus
  • KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162,
  • Another aspect of the invention relates to a method for improving drought resistance in plants, comprising producing a plurality of plants, plant protoplasts or plant cells that have been genetically engineered to overexpress or express de novo a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, wherein the KGDR06 protein comprises an amino acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53, and screening the genetically-engineered plants, plant protoplasts or plant cells for improved drought resistance and selecting a plant, plant protoplast or plant cell having improved drought resistance, wherein the KGDR26 protein comprises an amino acid sequence having at least 70%, at least 80%
  • Another aspect of the invention described herein pertains to a recombinant nucleic acid comprising a nucleotide sequence encoding a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, operably linked to a promoter.
  • the recombinant nucleic acid comprising a nucleotide sequence encoding a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, operably linked to a heterologous promoter.
  • the nucleotide sequence encodes a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the nucleotide sequence has at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 28, 30, 32, 34, 36, 38, 44, 46, 48, 50, 52, and 54.
  • the nucleotide sequence encodes a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the nucleotide sequence has at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the nucleotide sequence encodes a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15 .
  • the nucleotide sequence has at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the nucleotide sequence encodes a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125. In some embodiments, the nucleotide sequence has at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the nucleotide sequence encodes a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146,
  • the nucleotide sequence has at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 127, 129, 131 , 133, 135, 137, 139, 141 , 143, 145, 147,
  • the recombinant nucleic acid comprises a cDNA sequence encoding the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein or any combination thereof. In some embodiments, the recombinant nucleic acid comprises a CDS sequence encoding the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof.
  • the promoter is active in plant cells.
  • the promoter is a heterologous promoter or is not operably linked to a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene, respectively, in naturally-occurring species.
  • the promoter is operably linked to a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene, respectively, in naturally-occurring species.
  • the promoter is a constitutive promoter.
  • the promoter is an inducible promoter.
  • Another aspect of the invention described herein pertains to a vector or expression construct comprising the recombinant nucleic acid.
  • the vector or expression construct is configured for Agrobacterium-mediated transformation.
  • a plant with improved drought resistance can also be obtained by intentional introgression of at least one (additional) copy of a KGDR06, KGDR26,
  • Introgression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene, or any combination thereof, can be achieved by repeated backcrossing of an interspecific hybrid comprising the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene, or any combination thereof with one of its parent species to stabilize the overexpression or de novo expression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene, or any combination thereof, in that species.
  • KGDR06 KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, encoding nucleic acid or encoding expression construct encoding said protein, for improving drought resistance in plants, preferably in a method of the invention as defined herein.
  • a further aspect of the invention pertains to a plant, plant protoplast or plant cell having improved drought resistance obtainable or obtained by the method described herein.
  • the plant, plant protoplast or plant cell may be any plant, plant protoplast or plant cell, or may be derived from any plant, such as monocotyledonous plants or dicotyledonous plants, preferably of the family Solanaceae. In some embodiments, the plant is selected
  • the plant is selected from Tobacco (Nicotianaspecies, e.g. N. benthamiana, N.
  • plumbaginifolia N. tabacum, etc.
  • vegetable species including tomato (Solanum lycopersicum) such as e.g. cherry tomato, var. cerasiforme or currant tomato, var. pimpinellifolium) or tree tomato (S.
  • betaceum syn. Cyphomandra betaceae), potato (Solanum tuberosum), eggplant (Solanum
  • the plant cell of the invention may be a cell that does not possess the property of photosynthesis.
  • the plant may belong to any other family, such as Cucurbitaceae or
  • the plant is selected from maize/corn (Zea species), wheat (Triticum species), barley (e.g. Hordeum vulgare), oat (e.g. Avena sativa), sorghum (Sorghum bicolor), rye (Secale cereale), soybean (Glycine spp., e.g. G. max), cotton (Gossypium species, e.g. G.
  • Brassica spp. e.g. B. napus, B. juncea, B. oleracea, B. rapa, etc
  • sunflower Helianthus annus
  • safflower e.g. 0. sativa indica cultivar-group or japonica cultivar-group
  • forage grasses pearl millet
  • pearl millet e.g. P.
  • glaucum tree species (Pinus, poplar, fir, plantain, etc.), tea, coffea, oil palm, coconut, vegetable species, such as pea, zucchini, beans (e.g. Phaseolus species), cucumber, artichoke, asparagus, broccoli, garlic, leek, lettuce, onion, radish, turnip, Brussels sprouts, carrot, cauliflower, chicory, celery, spinach, endive, fennel, beet, fleshy fruit bearing plants (grapes, peaches, plums, strawberry, mango, apple, plum, cherry, apricot, banana, blackberry, blueberry, citrus, kiwi, figs, lemon, lime, nectarines, raspberry, watermelon, orange, grapefruit, etc.), ornamental species (e.g.
  • Rose Petunia, Chrysanthemum, Lily, Gerbera species
  • herbs mint, parsley, basil, thyme, etc.
  • woody trees e.g. species of Populus, Salix, Quercus, Eucalyptus
  • fibre species e.g. flax (Linum usitatissimum) and hemp (Cannabis sativa)
  • model organisms such as Arabidopsis thaliana.
  • the plant is selected from "crop plants", i.e. plant species which is cultivated and bred by humans.
  • a crop plant may be cultivated for food purposes (e.g. field crops), or for ornamental purposes (e.g. production of flowers for cutting, grasses for lawns, etc.).
  • a crop plant as defined herein also includes plants from which non-food products are harvested, such as oil for fuel, plastic polymers, pharmaceutical products, cork and the like.
  • the plant overexpresses or expresses de novo a KGDR06 protein that comprises an amino acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant overexpresses or expresses de novo a KGDR06 protein encoded by a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 2, 4, 6, 8, 10, 12,
  • the plant is a corn plant that overexpresses an endogenous
  • KGDR06 protein e.g., SEQ ID NOs: 3, 5, 7, and 9 or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 3, 5, 7, and 9.
  • the plant is a corn plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a wheat plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NO: 1 1 ) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 1 1 .
  • the plant is a wheat plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 13,
  • the plant is a rice plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NOs: 13, 15, and 17) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 13, 15, and 17.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 13, 15, and 17
  • the plant is a rice plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a sorghum plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NOs: 19, 21 , and 23) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 19, 21 , and 23.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 19, 21 , and 23
  • the plant is a sorghum plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a yam plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NOs: 25 and/or 26) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 25 and/or 26.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 25 and/or 26
  • a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 25 and/or 26.
  • the plant is a yam plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a plantain/banana plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NOs: 27, 29, 31 , and 33) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 27, 29, 31 , and 33.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 27, 29, 31 , and 33
  • the plant is a plantain plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25, 26, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a potato plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NOs: 35 and 37) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least
  • the plant is a potato plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a cassava plant that overexpresses an endogenous
  • KGDR06 protein e.g., SEQ ID NOs: 39, 40, 41 , and 42
  • KGDR06 protein expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 39, 40, 41 , and 42.
  • the plant is a cassava plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 43, 45, 47, 49, 51 , and 53.
  • the plant is a soybean plant that overexpresses an endogenous KGDR06 protein (e.g., SEQ ID NOs: 43, 45, 47, 49, 51 , and 53) or expresses de novo a homologous KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 43, 45, 47, 49, 51 , and 53.
  • an endogenous KGDR06 protein e.g., SEQ ID NOs: 43, 45, 47, 49, 51 , and 53
  • the plant is a soybean plant that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, and 39-42.
  • the plant is a tomato plant (e.g., Solanum lycopersicum) that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a tomato plant e.g., Solanum lycopersicum
  • the plant is a cotton plant (e.g., Gossypium hirsutum) that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a cotton plant e.g., Gossypium hirsutum
  • the plant is a barley plant (e.g., Hordeum vulgare) that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a barley plant e.g., Hordeum vulgare
  • the plant is an oat plant (e.g., Avena sativa) that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • Avena sativa expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • the plant is a rye plant (e.g., Secale cereale) that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a rye plant e.g., Secale cereale
  • the plant is a rapeseed plant (e.g., Brassica napus) that expresses de novo a KGDR06 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a rapeseed plant e.g., Brassica napus
  • the plant is selected from "crop plants", i.e. plant species which is cultivated and bred by humans.
  • a crop plant may be cultivated for food purposes (e.g. field crops), or for ornamental purposes (e.g. production of flowers for cutting, grasses for lawns, etc.).
  • a crop plant as defined herein also includes plants from which non-food products are harvested, such as oil for fuel, plastic polymers, pharmaceutical products, cork and the like.
  • the plant overexpresses or expresses de novo a KGDR26 protein that comprises an amino acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the plant overexpresses or expresses de novo a KGDR26 protein encoded by a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88 and 90.
  • the plant is a corn plant that overexpresses an endogenous KGDR26 protein (e.g., SEQ ID NO: 57) or expresses de novo a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 57.
  • an endogenous KGDR26 protein e.g., SEQ ID NO: 57
  • SEQ ID NO: 57 an endogenous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 57.
  • the plant is a corn plant that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 59, 61 , 63, 65,
  • the plant is a wheat plant (e.g., Triticum aestivum) that overexpresses an endogenous KGDR26 protein (e.g., SEQ ID NO: 59) or expresses de novo a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 59.
  • an endogenous KGDR26 protein e.g., SEQ ID NO: 59
  • SEQ ID NO: 59 an endogenous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NO: 59.
  • the plant is a wheat plant that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the plant is a sorghum plant (e.g., Sorghum bicolor) that overexpresses an endogenous KGDR26 protein (e.g., SEQ ID NO: 61 ) or expresses de novo a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 61.
  • Sorghum bicolor an endogenous KGDR26 protein
  • the plant is a sorghum plant that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the plant is a potato plant that overexpresses an endogenous KGDR26 protein (e.g., SEQ ID NOs: 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 and 83) or expresses de novo a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 and 83.
  • an endogenous KGDR26 protein e.g., SEQ ID NOs: 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 and 83
  • the plant is a potato plant that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 85, 87 and 89.
  • the plant is a soybean plant that overexpresses an endogenous KGDR26 protein (e.g., SEQ ID NOs: 85, 87 and 89) or expresses de novo a homologous KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 85, 87 and 89.
  • an endogenous KGDR26 protein e.g., SEQ ID NOs: 85, 87 and 89
  • the plant is a soybean plant that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 and 83.
  • the plant is a rice plant (e.g., Oryza sativa) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • rice plant e.g., Oryza sativa
  • the plant is a yam plant (e.g., Dioscorea spp.) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a yam plant e.g., Dioscorea spp.
  • the plant is a plantain or banana plant (e.g., Musa spp.) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • banana plant e.g., Musa spp.
  • the plant is a cassava plant (e.g., Manihot esculenta) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • cassava plant e.g., Manihot esculenta
  • the plant is a tomato plant (e.g., Solanum lycopersicum) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a tomato plant e.g., Solanum lycopersicum
  • the plant is a cotton plant (e.g., Gossypium hirsutum) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a cotton plant e.g., Gossypium hirsutum
  • the plant is a barley plant (e.g., Hordeum vulgare) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a barley plant e.g., Hordeum vulgare
  • the plant is an oat plant (e.g., Avena sativa) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • Avena sativa expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • the plant is a rye plant (e.g., Secale cereale) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a rye plant e.g., Secale cereale
  • the plant is a rapeseed plant (e.g., Brassica napus) that expresses de novo a KGDR26 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a rapeseed plant e.g., Brassica napus
  • the plant overexpresses or expresses de novo a KGDR25 protein that comprises an amino acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15 .
  • the plant overexpresses or expresses de novo a KGDR25 protein encoded by a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 92, 94, 96, 98, 101 , 103, 105, 108, 1 10, 1 14 and 1 16.
  • the plant is a corn plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NOs: 93, 95 and 97) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 93, 95 and 97.
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 93, 95 and 97
  • the plant is a corn plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a wheat plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NOs: 99, 100, or 102) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 99, 100, and 102.
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 99, 100, or 102
  • the plant is a wheat plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a sorghum plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NO: 104) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 104.
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 104
  • SEQ ID NO: 104 an endogenous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 104.
  • the plant is a sorghum plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99- 100, 102, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a yam plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NO: 106) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 106.
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 106
  • SEQ ID NO: 106 an endogenous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 106.
  • the plant is a yam plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99- 100, 102, 104, 107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a plantain plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NO: 107) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 107.
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 107
  • SEQ ID NO: 107 an endogenous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 107.
  • the plant is a plantain plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99- 100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a potato plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NO: 109) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 109.
  • an endogenous KGDR25 protein e.g., SEQ ID NO: 109
  • SEQ ID NO: 109 an endogenous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 109.
  • the plant is a potato plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99- 100, 102, 104, 106-107, 1 1 1-1 13 and 1 15.
  • the plant is a cassava plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NOs: 1 1 1 and 1 12) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 1 1 and 1 12.
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 1 1 1 and 1 12
  • SEQ ID NOs: 1 1 1 and 1 12 an endogenous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 1 1 and 1 12.
  • the plant is a cassava plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 13 and 1 15.
  • the plant is a soybean plant that overexpresses an endogenous KGDR25 protein (e.g., SEQ ID NOs: 1 13 and 1 15) or expresses de novo a homologous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 13 and 1 15.
  • an endogenous KGDR25 protein e.g., SEQ ID NOs: 1 13 and 1 15
  • SEQ ID NOs: 1 13 and 1 15 an endogenous KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 1 13 and 1 15.
  • the plant is a soybean plant that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 12.
  • the plant is a rice plant (e.g., Oryza sativa) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106- 107, 109, 1 1 1-1 13 and 1 15.
  • a rice plant e.g., Oryza sativa
  • KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106- 107, 109, 1 1 1 1-1 13 and 1 15.
  • the plant is a tomato plant (e.g., Solanum lycopersicum) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99- 100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • a tomato plant e.g., Solanum lycopersicum
  • the plant is a cotton plant (e.g., Gossypium hirsutum) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99- 100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • a cotton plant e.g., Gossypium hirsutum
  • the plant is a barley plant (e.g., Hordeum vulgare) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • a barley plant e.g., Hordeum vulgare
  • a barley plant e.g., Hordeum vulgare
  • the plant is an oat plant (e.g., Avena sativa) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • Avena sativa expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a rye plant (e.g., Secale cereale) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • a rye plant e.g., Secale cereale
  • a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant is a rapeseed plant (e.g., Brassica napus) that expresses de novo a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • a rapeseed plant e.g., Brassica napus
  • a KGDR25 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15.
  • the plant overexpresses or expresses de novo a KGDR42 protein that comprises an amino acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant overexpresses or expresses de novo a KGDR42 protein encoded by a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 18, 120, 122 and 124.
  • the plant is a corn plant that overexpresses an endogenous KGDR42 protein (e.g., SEQ ID NOs: 1 19 and 121 ) or expresses de novo a homologous KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 19 and 121.
  • the plant is a corn plant that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of 1 17, 123 and 125.
  • the plant is a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant.
  • Triticum aestivum e.g., Triticum aestivum
  • the plant is a wheat plant that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 and 125.
  • the plant is a yam plant (e.g., Dioscorea spp.) that overexpresses an endogenous KGDR42 protein (e.g., SEQ ID NO: 125) or expresses de novo a homologous KGDR42 protein (e.g., SEQ ID NO: 125) or expresses de novo a homologous KGDR42 protein (e.g., SEQ ID NO: 125) or expresses de novo a homologous
  • the plant is a yam plant that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 and 123.
  • the plant is a rice plant (e.g., Oryza sativa) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a rice plant e.g., Oryza sativa
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant is a sorghum plant (e.g., Sorghum bicolor) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • sorghum plant e.g., Sorghum bicolor
  • the plant is a plantain or banana plant (e.g., Musa spp.) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a plantain or banana plant e.g., Musa spp.
  • the plant is a potato plant (e.g., Solanum tuberosum) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a potato plant e.g., Solanum tuberosum
  • the plant is a cassava plant (e.g., Manihot esculenta) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a cassava plant e.g., Manihot esculenta
  • the plant is a soybean plant (e.g., Glycine max) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • Glycine max a soybean plant that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant is a tomato plant (e.g., Solanum lycopersicum) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a tomato plant e.g., Solanum lycopersicum
  • the plant is a cotton plant (e.g., Gossypium hirsutum) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a cotton plant e.g., Gossypium hirsutum
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant is a barley plant (e.g., Hordeum vulgare) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a barley plant e.g., Hordeum vulgare
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant is an oat plant (e.g., Avena sativa) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • oat plant e.g., Avena sativa
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant is a rye plant (e.g., Secale cereale) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a rye plant e.g., Secale cereale
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant is a rapeseed plant (e.g., Brassica napus) that expresses de novo a KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a rapeseed plant e.g., Brassica napus
  • KGDR42 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • the plant overexpresses or expresses de novo a KGDR37 protein that comprises an amino acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130,
  • the plant overexpresses or expresses de novo a KGDR37 protein encoded by a nucleotide sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 127, 129, 131 ,
  • the plant is a corn plant that overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NOs: 128, 130, or 132) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 128, 130, and 132.
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 128, 130, or 132
  • the plant is a corn plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the plant is a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant (e.g., Triticum aestivum) that has a wheat plant.
  • Triticum aestivum e.g., Triticum aestivum
  • KGDR37 protein overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NOs: 134, 136, 138, 140, 142 and 144) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs:
  • the plant is a wheat plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the plant is a rice plant (e.g., Oryza sativa) that expresses an endogenous KGDR37 protein (e.g., SEQ ID NO: 146) or expressing de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 146.
  • an endogenous KGDR37 protein e.g., SEQ ID NO: 146
  • the plant is a rice plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the plant is a sorghum plant that overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NO: 148) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 148.
  • an endogenous KGDR37 protein e.g., SEQ ID NO: 1408
  • SEQ ID NO: 1408 an endogenous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 148.
  • the plant is a sorghum plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 150-154, 156, 158-162, 164, 166 and 168.
  • the plant is a yam plant that overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NOs: 150-153) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 150-153.
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 150-153
  • SEQ ID NOs: 150-153 an endogenous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 150-153.
  • the plant is a yam plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 154, 156, 158-162, 164, 166 and 168.
  • the plant is a potato plant that overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NOs: 154 and 156) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 154 and 156.
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 154 and 156
  • SEQ ID NOs: 154 and 156 expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NO: 154 and 156.
  • the plant is a potato plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-153, 158-162, 164, 166 and 168.
  • the plant is a cassava plant that overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NOs: 158-161 ) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 158-161.
  • the plant is a cassava plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least
  • the plant is a soybean plant that overexpresses an endogenous KGDR37 protein (e.g., SEQ ID NOs: 162, 164, 166 and 168) or expresses de novo a homologous KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with SEQ ID NOs: 162, 164, 166 and 168.
  • an endogenous KGDR37 protein e.g., SEQ ID NOs: 162, 164, 166 and 168
  • the plant is a soybean plant that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-161.
  • the plant is a plantain or banana plant (e.g., Musa spp.) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a plantain or banana plant e.g., Musa spp.
  • the plant is a tomato plant (e.g., Solanum lycopersicum) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a tomato plant e.g., Solanum lycopersicum
  • the plant is a cotton plant (e.g., Gossypium hirsutum) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a cotton plant e.g., Gossypium hirsutum
  • the plant is a barley plant (e.g., Hordeum vulgare) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a barley plant e.g., Hordeum vulgare
  • the plant is an oat plant (e.g., Avena sativa) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • Avena sativa expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • the plant is a rye plant (e.g., Secale cereale) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a rye plant e.g., Secale cereale
  • the plant is a rapeseed plant (e.g., Brassica napus) that expresses de novo a KGDR37 protein having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • rapeseed plant e.g., Brassica napus
  • the plant with improved drought resistance is a plant obtained by genetic engineering. In some embodiments, the plant with improved drought resistance is a plant obtained by intentional introgression of at least one (additional) copy of a KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene, or any combination thereof, into the genome of the plant through breeding and selection.
  • An additional aspect of the invention described herein pertains to seeds produced by the plant with improved drought resistance.
  • An additional aspect of the invention described herein pertains to plants grown from the seeds or regenerated from the plant cell or plant protoplast.
  • An additional aspect of the invention described herein pertains to progenies of the plant with improved drought resistance obtained by breeding and selection, wherein the selected progenies retain the improved drought resistance of the parent and/or retain the overexpression or de novo expression of KGDR06, KGDR26, KGDR25, KGDR42, KGDR37, or any combination thereof.
  • the invention also provides products derived from the plant having improved drought resistance as defined herein, e.g. fruits, leaves, plant organs, plant fats, plant oils, plant starch, and plant protein fractions, either crushed, milled or still intact, mixed with other materials, dried, frozen , and so on. These products may be non-propagating.
  • these products comprise at least fractions of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein, or any combination thereof, or encoding nucleic acid as defined herein, which allows to assess that the plant product is derived from a plant of the invention, e.g. a gene that has been modified such that the it results in overexpression or expressing de novo a KGDR06 protein.
  • a further aspect of the invention described herein provides a KGDR06 variant or altered KGDR06 protein that comprises an amino acid seguence having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% seguence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53, a KGDR26 variant or altered KGDR26 protein that comprises an amino acid seguence having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85,
  • amino acid sequences of the altered KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein and a respective wildtype KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 protein may differ by one or more deletions, additions, and/or substitutions of amino acids while retaining functional equivalence to the polypeptide.
  • such proteins comprise from 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10 or more up to about 100, 90, 80, 70, 60, 50, 45, 40, 35, 30, 25, 20, 15 amino acid substitutions, deletions or insertions.
  • amino acids of the polypeptide of the invention may be modified based on similarity in hydrophobicity, hydrophilicity, solubility, polarity of amino acid residues, as long as the variant polypeptide remains functionally equivalent to the polypeptide of the invention.
  • a variant may differ from the polypeptide of the invention by attachment of modifying groups which are covalently or non-covalently linked to the polypeptide backbone.
  • the variant also includes a polypeptide which differs from the polypeptide of the present invention by introduced N-linked or O-linked glycosylation sites, and/or an addition of cysteine residues.
  • DNASTAR DNASTAR
  • a further aspect of the invention described herein provides an isolated, recombinant or synthetic nucleotide sequence encoding a wildtype KGDR06 protein or a KGDR06 variant or altered KGDR06 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53.
  • a further aspect of the invention described herein provides an expression construct comprising a nucleotide sequence encoding a wildtype KGDR06 protein or a KGDR06 variant or altered KGDR06 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25-27, 29, 31 , 33, 35, 37, 39-43, 45, 47, 49, 51 , and 53, which nucleotide sequence is operably linked to a promoter such as a heterologous promoter.
  • a further aspect of the invention described herein provides an isolated, recombinant or synthetic nucleotide sequence encoding a wildtype KGDR26 protein or a KGDR26 variant or altered KGDR26 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89.
  • a further aspect of the invention described herein provides an expression construct comprising a nucleotide sequence encoding a wildtype KGDR26 protein or a KGDR26 variant or altered KGDR26 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 55, 57, 59, 61 , 63, 65, 67, 69, 71 , 73, 75, 77, 79, 81 , 83, 85, 87 and 89, which nucleotide sequence is operably linked to a promoter such as a heterologous promoter.
  • a further aspect of the invention described herein provides an isolated, recombinant or synthetic nucleotide sequence encoding a wildtype KGDR25 protein or a KGDR25 variant or altered KGDR25 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1- 1 13 and 1 15 .
  • a further aspect of the invention described herein provides an expression construct comprising a nucleotide sequence encoding a wildtype KGDR25 protein or a KGDR25 variant or altered KGDR25 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 91 , 93, 95, 97, 99-100, 102, 104, 106-107, 109, 1 1 1-1 13 and 1 15 , which nucleotide sequence is operably linked to a promoter such as a heterologous promoter.
  • a further aspect of the invention described herein provides an isolated, recombinant or synthetic nucleotide sequence encoding a wildtype KGDR42 protein or a KGDR42 variant or altered KGDR42 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125.
  • a further aspect of the invention described herein provides an expression construct comprising a nucleotide sequence encoding a wildtype KGDR42 protein or a KGDR42 variant or altered KGDR42 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 1 17, 1 19, 121 , 123 and 125, which nucleotide sequence is operably linked to a promoter such as a heterologous promoter.
  • a further aspect of the invention described herein provides an isolated, recombinant or synthetic nucleotide sequence encoding a wildtype KGDR37 protein or a KGDR37 variant or altered KGDR37 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168.
  • a further aspect of the invention described herein provides an expression construct comprising a nucleotide sequence encoding a wildtype KGDR37 protein or a KGDR37 variant or altered KGDR37 protein having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% sequence identity with any of SEQ ID NOs: 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 and 168, which nucleotide sequence is operably linked to a promoter such as a heterologous promoter.
  • KGDR06 Arabidopsis drought resistance genes KGDR06 (At5G64660), KGDR26 (At5G17880), KGDR25 (At5G16140), KGDR42 (At5G17880) and KGDR37 (At1 G49300) were obtained from online databases (e.g., Arabidopsis.org).
  • the corresponding primers was designed based on the DNA sequence and used to amplify the full length cDNA by RT-PCR. RNA isolated from Arabidopsis seedlings was used as template in the RT-PCR reaction.
  • the sequence was cloned into a pCambia-based binary vector, which includes nptll as a plant selection marker.
  • the constitutive promoter tCUP on the vector can drive the expression of the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene in the plant (Wu et al., Plant J., 2003, 34 (2):241-247).
  • Either one of the KGDR06-, KGDR26-, KGDR25-, KGDR42- or KGDR37-expressing vector was transformed into a potato cultivar-Ranger Russet.
  • An Agrobacterium culture containing the construct in a frozen glycerol stock was inoculated in the Luria broth (LB) liquid medium with selective antibiotic. Cultures were maintained overnight in an incubator at 28 °C with shaking. Then, the next morning, a 5 mL aliquot of overnight culture was transferred into an Erlenmeyer flask containing 15 mL of fresh LB and incubated for another 4-6 hours at 28 °C with shaking. The absorbance of the culture was measured using a spectrometer and adjusted to a final OD6oo nm of 0.2.
  • Potato stock plants were grown from the nodal or apical meristems for 4-5 weeks with CG medium (4.70 g/L MS modified potato medium, 15 g/L sucrose, 2.0 g/L gelzan, pH 5.7). Stem internodes (3-5 mm long) as explants were cut from stock plants and stored in a petri plate with 15 mL of MS liquid medium (4.44 g/L modified MS basal medium with Gamborg's vitamins (M404), 30 g/L sucrose, pH 5.7). When all explant material was cut, the MS liquid was decanted from the explants, and 15 to 25 mL of the Agrobacterium culture (OD6oo nm of 0.2) was added to the explants. The explants were gently swirled in the suspension of Agrobacterium for 10 minutes on an orbital shaker at the room temperature.
  • CG medium 4.70 g/L MS modified potato medium, 15 g/L sucrose, 2.0 g/L gelzan, pH 5.7.
  • the explants were then removed from the Agrobacterium culture and transferred to the co- culture medium (0.444 g/L modified MS basal medium with Gamborg's vitamins (M404, Phytotech), 30 g/L sucrose, agar 6 g/L (Phytotech), pH 5.7) for 48 hours.
  • co- culture medium 0.444 g/L modified MS basal medium with Gamborg's vitamins (M404, Phytotech), 30 g/L sucrose, agar 6 g/L (Phytotech), pH 5.7
  • the explants were transferred to a callus induction medium (4.44 g/L modified MS basal medium with Gamborg's vitamins (M404, Phytotech), 30 g/L sucrose, 2.5 mg/L zeatin riboside, 0.1 mg/L NAA, 100 mg/L kanamycin, 150 mg/L timentin, pH 5.7, 6 g/L agar) and incubated in a growth chamber at 24 °C with a 16-hour photoperiod.
  • a callus induction medium (4.44 g/L modified MS basal medium with Gamborg's vitamins (M404, Phytotech), 30 g/L sucrose, 2.5 mg/L zeatin riboside, 0.1 mg/L NAA, 100 mg/L kanamycin, 150 mg/L timentin, pH 5.7, 6 g/L agar
  • the explants were transferred to a shoot induction medium (4.44 g/L modified MS basal medium with Gamborg's vitamins (M404), 30 g/L sucrose, 2.5 mg/L zeatin riboside, 0.3 mg/L GA3, 100 mg/L kanamycin, 150 mg/L timentin, pH 5.7, 6 g/L agar) and placed in a growth chamber set at 24 °C with a 16-hour photoperiod. Explants were cultured for up to four weeks on shoot induction medium. As shoots form, they were removed from calli (1 shoot per explant end) and placed in magenta boxes for rooting on 100 mg/l kanamycin.
  • a shoot induction medium (4.44 g/L modified MS basal medium with Gamborg's vitamins (M404), 30 g/L sucrose, 2.5 mg/L zeatin riboside, 0.3 mg/L GA3, 100 mg/L kanamycin, 150 mg/L timentin, pH 5.7, 6
  • Ranger Russet plants expressing the KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 gene were planted to soil in greenhouses following the standard protocols for potato seed production. Resulting seed potatoes (nuclear) were certified by the Idaho Crop Improvement association as certified seed and planted in the field in La Grande, OR. Seeds were planted by hand (5-50 grams) at 15-20 cm deep, 25 cm in-row spacing on 86 cm centers. Standard agronomic practices were employed during the growing season and general phenotypic notes were collected. Plants were not subjected to water stress during the 2014 season. Fifteen individual lines were planted at 10 plants per line in an Augmented experimental design.
  • Potatoes were harvested with a small plot harvester on September 23, 2014, assessed for yield, and stored at 4 °C (95%+ RH). Tuber total yield was analyzed using SAS Augmented design script. Yields were further assessed by pooling all lines to give an average yield to identify the underlying effects of KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37. Distribution of event lines and variation were assessed.
  • Tubers from the 2014 La Grande, OR field trial were certified under Oregon State's Seed Certification Program and used as G1 seeds for the 2015 field trial in Parma, ID.
  • the seeds were cut and blocked for apical and basal portions (50-64 g) on April 2, 2015.
  • the seeds were allowed to wound heal at 12 °C (95%+ RH) until planting on April 14, 2015 in a Randomized Complete Block Design with five field replicates of ten plants per replicate.
  • the seeds were planted approximately 20 cm deep with 25 cm in-row spacing and with 91 cm centers using a research capacity two-row potato planter.
  • Parma field soil is characterized as a Greenleaf-Owyhee silt loam.
  • Pre-tuberization irrigation was managed via sprinkler irrigation for chemical applications and via drip irrigation post- tuberization for the remainder of the growing season.
  • the use of sprinklers facilitated application of dimethen amid-P (Outlook) and pendimethalin (Prowl) at rates of 0.98 kg/ha and 2.3 L/ha, respectively.
  • a pre-plant application of 179.4 kg/ha nitrogen (N) was applied as urea and was incorporated with sprinkler irrigation. In-season fertilizer injections were made via the drip irrigation system.
  • the drip irrigation system utilized Aqua-Traxx disposable tape (8 mm wall thickness; 5 mm pore size; EA5080820) from The Toro Company Micro-Irrigation Business (1588 N. Marshall Ave., El Cajon, CA 92020) to deliver water to the plants at a rate of 0.3 L per hour at 1.03 bars and was place 21 cm to the side of the seed piece.
  • DAP The Toro Company Micro-Irrigation Business
  • the initial drip irrigation treatment was ran for 36 hours to allow for salts and silts to be leached from around the drip tape to not limit the ability of the drip irrigation system to wet the soil profile throughout the growing season.
  • irrigation rates were applied at 50% of (approx.
  • Tukey's HSD, histograms and standard deviations were used to assess individual lines compared to the empty-vector control and wild-type. Yield data were initially evaluated by comparing differences in population distribution for the plants expressing KGDR06, KGDR26, KGDR25, KGDR42 or KGDR37 as compared to the empty-vector control and wild-type. The average yield of plants expressing KGDR06 was higher than the empty-vector control under drought conditions. The plants expressing KGDR06 demonstrated a normal and tighter distribution, as compared to the controls, which suggested that the response was an effect of the KGDR06 gene and not inherit variability.
  • the average yield of plants expressing KGDR26 was higher than both the empty-vector control and wild- type under drought conditions.
  • the plants expressing KGDR26 demonstrated a normal and tighter distribution, as compared to the controls, which suggested that the response was an effect of the KGDR26 gene and not inherit variability.
  • the average yield of plants expressing KGDR25 was higher than both the empty-vector control and wild-type under drought conditions.
  • the plants expressing KGDR25 demonstrated a normal and tighter distribution, as compared to the controls, which suggested that the response was an effect of the KGDR25 gene and not inherit variability.
  • the average yield of plants expressing KGDR42 was higher than both the empty-vector control and wild-type under drought conditions.
  • the plants expressing KGDR42 demonstrated a normal and tighter distribution, as compared to the controls, which suggested that the response was an effect of the KGDR42 gene and not inherit variability.
  • the average yield of plants expressing KGDR37 was higher than both the empty- vector control and wild-type under drought conditions.
  • the plants expressing KGDR37 demonstrated a normal and tighter distribution, as compared to the controls, which suggested that the response was an effect of the KGDR37 gene and not inherit variability.
  • At least three individual lines expressing KGDR26 exhibited significant higher yield under drought conditions compared to the empty-vector control (about 18-27% increase in yield), and at least one individual line expressing KGDR26 exhibited significant higher yield under drought conditions compared to the wild- type (about 16% increase in yield).
  • At least four individual lines expressing KGDR25 exhibited significant higher yield under drought conditions compared to the empty-vector control (about 18-28% increase in yield), and at least one individual line expressing KGDR25 exhibited significant higher yield under drought conditions compared to the wild-type (about 17% increase in yield).
  • At least three individual lines expressing KGDR42 exhibited significant higher yield under drought conditions compared to the empty-vector control (about 17-30% increase in yield), and at least one individual line expressing KGDR42 exhibited significant higher yield under drought conditions compared to the wild- type (about 19% increase in yield).
  • At least four individual lines expressing KGDR37 exhibited significant higher yield under drought conditions compared to the empty-vector control (about 19-95% increase in yield), and at least two individual lines expressing KGDR37 exhibited significant higher yield under drought conditions compared to the wild-type (about 18-79% increase in yield).
  • the average of all lines expressing KGDR06 (1.78 kg/plant) had higher yields under drought conditions than the empty vector control (1.64 kg/plant) at p ⁇ 0.05.
  • the terms “substantially,” “substantial,” and “about” are used to describe and account for small variations.
  • the terms can refer to instances in which the event or circumstance occurs precisely as well as instances in which the event or circumstance occurs to a close approximation.
  • the terms can refer to less than or equal to ⁇ 10%, such as less than or equal to ⁇ 5%, less than or equal to ⁇ 4%, less than or equal to ⁇ 3%, less than or equal to ⁇ 2%, less than or equal to ⁇ 1 %, less than or equal to ⁇ 0.5%, less than or equal to ⁇ 0.1 %, or less than or equal to ⁇ 0.05%.

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Abstract

L'invention concerne un procédé d'amélioration de la résistance à la sécheresse dans des plantes, comprenant la surexpression ou l'expression de la protéine de novoa KGDR06, une protéine KGDR26, une protéine KGDR25, une protéine KGDR42, une protéine KGDR37, ou toute combinaison de celles-ci, dans une plante, un protoplaste végétal ou une cellule végétale, la protéine KGDR06 comprenant une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25-27, 29, 31, 33, 35, 37, 39-43, 45, 47, 49, 51, et 53, la protéine KGDR26 comprenant une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87 et 89, la protéine KGDR25 comprenant une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 91, 93, 95, 97, 99-100, 102, 104, 106-107, 109, 111-113 et 115, la protéine KGDR42 comprenant une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 117, 119, 121, 123 et 125, et la protéine KGDR37 comprenant une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 et 168. L'invention concerne également une plante, un protoplaste végétal ou une cellule végétale ayant une résistance à la sécheresse améliorée, la plante, le protoplaste végétal ou la cellule végétale a été génétiquement modifié pour surexprimer ou exprimer une protéine de novoa KGDR06 qui comprend une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25-27, 29, 31, 33, 35, 37, 39-43, 45, 47, 49, 51, et 53, une protéine KGDR26 qui comprend une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87 et 89, une protéine KGDR25 qui comprend une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 91, 93, 95, 97, 99-100, 102, 104, 106-107, 109, 11-113 et 115, une protéine KGDR42 qui comprend une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 117, 119, 121, 123 et 125, une protéine KGDR37 qui comprend une séquence d'acides aminés ayant au moins 85 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150-154, 156, 158-162, 164, 166 et 168, ou toute combinaison de ceux-ci.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109734786A (zh) * 2018-12-10 2019-05-10 北京市农林科学院 植物花粉育性恢复相关蛋白TaDMT25及其编码基因和应用

Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006722A1 (fr) 1993-09-03 1995-03-09 Japan Tobacco Inc. Procede permettant de transformer une monocotyledone avec un scutellum d'embryon immature
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
US20020138879A1 (en) 1997-04-30 2002-09-26 Tishu Cai Agrobacterium mediated transformed sorghum
WO2002083911A1 (fr) 2001-04-12 2002-10-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Production de plantes a tolerance amelioree au stress de secheresse ou a transpiration augmentee
WO2003020015A2 (fr) 2001-08-30 2003-03-13 Purdue Research Foundation Procedes de production de plantes transgeniques resistant aux contraintes osmotiques
WO2003081988A2 (fr) * 2002-03-27 2003-10-09 Agrinomics Llc Production de plantes a tolerance amelioree vis-a-vis de la secheresse
US20060150283A1 (en) * 2004-02-13 2006-07-06 Nickolai Alexandrov Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
US20070266453A1 (en) 2003-06-24 2007-11-15 Catherine Anderson Generation of Plants with Improved Drought Tolerance
US20110191912A1 (en) * 2000-04-26 2011-08-04 Nickolai Alexandrov Promoter, promoter control elements, and combinations, and uses thereof
WO2013006058A1 (fr) * 2011-07-07 2013-01-10 Keygene N.V. Utilisation de jaz5a pour l'amélioration de la résistance à la sécheresse chez une plante
WO2013122473A1 (fr) 2012-02-17 2013-08-22 Keygene N.V. Amélioration de la résistance des plantes à la sécheresse: pectinestérase
WO2013122471A1 (fr) 2012-02-17 2013-08-22 Keygene N.V. Amélioration de la résistance de plantes à la sécheresse : upl4
WO2013122472A1 (fr) 2012-02-17 2013-08-22 Keygene N.V. Amélioration de la résistance de plantes à la sécheresse : upl3
WO2014184193A2 (fr) * 2013-05-13 2014-11-20 Consejo Superior De Investigaciones Cientificas (Csic) Plantes transgéniques
WO2016054978A1 (fr) * 2014-10-11 2016-04-14 山东农业大学 Gène associé à la résistance à la sécheresse osdt11 du riz et application de ce dernier

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
WO1995006722A1 (fr) 1993-09-03 1995-03-09 Japan Tobacco Inc. Procede permettant de transformer une monocotyledone avec un scutellum d'embryon immature
US20020138879A1 (en) 1997-04-30 2002-09-26 Tishu Cai Agrobacterium mediated transformed sorghum
US20110191912A1 (en) * 2000-04-26 2011-08-04 Nickolai Alexandrov Promoter, promoter control elements, and combinations, and uses thereof
WO2002083911A1 (fr) 2001-04-12 2002-10-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Production de plantes a tolerance amelioree au stress de secheresse ou a transpiration augmentee
WO2003020015A2 (fr) 2001-08-30 2003-03-13 Purdue Research Foundation Procedes de production de plantes transgeniques resistant aux contraintes osmotiques
US20090144850A1 (en) 2002-03-27 2009-06-04 Agrigenetics, Inc. Generation of plants with improved drought tolerance
WO2003081988A2 (fr) * 2002-03-27 2003-10-09 Agrinomics Llc Production de plantes a tolerance amelioree vis-a-vis de la secheresse
US20070266453A1 (en) 2003-06-24 2007-11-15 Catherine Anderson Generation of Plants with Improved Drought Tolerance
US20060150283A1 (en) * 2004-02-13 2006-07-06 Nickolai Alexandrov Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
WO2013006058A1 (fr) * 2011-07-07 2013-01-10 Keygene N.V. Utilisation de jaz5a pour l'amélioration de la résistance à la sécheresse chez une plante
WO2013122473A1 (fr) 2012-02-17 2013-08-22 Keygene N.V. Amélioration de la résistance des plantes à la sécheresse: pectinestérase
WO2013122471A1 (fr) 2012-02-17 2013-08-22 Keygene N.V. Amélioration de la résistance de plantes à la sécheresse : upl4
WO2013122472A1 (fr) 2012-02-17 2013-08-22 Keygene N.V. Amélioration de la résistance de plantes à la sécheresse : upl3
WO2014184193A2 (fr) * 2013-05-13 2014-11-20 Consejo Superior De Investigaciones Cientificas (Csic) Plantes transgéniques
WO2016054978A1 (fr) * 2014-10-11 2016-04-14 山东农业大学 Gène associé à la résistance à la sécheresse osdt11 du riz et application de ce dernier

Non-Patent Citations (24)

* Cited by examiner, † Cited by third party
Title
"BIOCOMPUTING: INFORMATICS AND GENOME PROJECTS", 1993, ACADEMIC PRESS
"COMPUTATIONAL MOLECULAR BIOLOGY", 1988, OXFORD UNIVERSITY PRESS
"COMPUTER ANALYSIS OF SEQUENCE DATA", 1994, HUMANA PRESS
"GUIDE TO HUGE COMPUTERS", 1994, ACADEMIC PRESS
"Methods in Enzymology", ACADEMIC PRESS
"SEQUENCE ANALYSIS PRIMER", 1991, M STOCKTON PRESS
ALBERT L. LEHNINGER: "Principles of Biochemistry", 1982, WORTH PUB., pages: 793 - 800
ATSCHUL, S. F. ET AL., J. MOLEC. BIOL., vol. 215, 1990, pages 403
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
CARILLO, H.; LIPTON, D., SIAM J. APPLIED MATH, vol. 48, 1988, pages 1073
DANBY ET AL., TRENDS IN BIOT., vol. 23, no. 11, 2005
DATABASE EMBL [online] 12 February 2016 (2016-02-12), "Sequence 32756 from patent US 20111/91912", retrieved from EBI accession no. EM_PAT:HK817483 Database accession no. HK817483 *
DATABASE Geneseq [online] 3 February 2011 (2011-02-03), "Arabidopsis thaliana polynucleotide SEQ ID NO 107560", retrieved from EBI accession no. GSN:AOE94084 Database accession no. AOE94084 *
DATABASE Geneseq [online] 3 February 2011 (2011-02-03), "Arabidopsis thaliana protein SEQ ID NO 118800", retrieved from EBI accession no. GSP:AOF05325 Database accession no. AOF05325 *
DEVEREUX, J. ET AL., NUCLEIC ACIDS RESEARCH, vol. 12, no. 1, 1984, pages 387
HARB ET AL., ANALYSIS OF DROUGHT STRESS IN ARABIDOPSIS, AOP, 2010
HEINJE, G.: "SEQUENCE ANALYSIS IN MOLECULAR BIOLOGY", 1987, ACADEMIC PRESS
KASUGA ET AL., NAT. BIOTECHNOL., vol. 17, no. 3, 1999, pages 287 - 291
KILIAN ET AL., PLANT J., vol. 50, no. 2, 2007, pages 347 - 363
SAMBROOK ET AL.: "Molecular Cloning. A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SERRANO ET AL., J. EXP. BOT., vol. 50, 1999, pages 1023 - 1036
SNOW; TINGEY, PLANT PHYSIOL., vol. 77, 1985, pages 602 - 7
WANG ET AL., PLANTA, vol. 218, no. 1, 2003, pages 1 - 14
WU ET AL., PLANT J., vol. 34, no. 2, 2003, pages 241 - 247

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CN109734786A (zh) * 2018-12-10 2019-05-10 北京市农林科学院 植物花粉育性恢复相关蛋白TaDMT25及其编码基因和应用
CN109734786B (zh) * 2018-12-10 2022-05-31 北京市农林科学院 植物花粉育性恢复相关蛋白TaDMT25及其编码基因和应用

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