WO2018069741A3 - Antibodies to a specific phosphorylation of u3kqk0 and its uses - Google Patents

Antibodies to a specific phosphorylation of u3kqk0 and its uses Download PDF

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Publication number
WO2018069741A3
WO2018069741A3 PCT/GR2017/000061 GR2017000061W WO2018069741A3 WO 2018069741 A3 WO2018069741 A3 WO 2018069741A3 GR 2017000061 W GR2017000061 W GR 2017000061W WO 2018069741 A3 WO2018069741 A3 WO 2018069741A3
Authority
WO
WIPO (PCT)
Prior art keywords
u3kqk0
antibodies
strand breaks
dna double
specific phosphorylation
Prior art date
Application number
PCT/GR2017/000061
Other languages
French (fr)
Other versions
WO2018069741A2 (en
Inventor
Efthymia ROGAKOU
Original Assignee
Epigenfocus Ike
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenfocus Ike filed Critical Epigenfocus Ike
Publication of WO2018069741A2 publication Critical patent/WO2018069741A2/en
Publication of WO2018069741A3 publication Critical patent/WO2018069741A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a specific antibody against the phosphorylated serine at position 157 of the U3KQK0 protein. Said selective binding is indicative of the existence of DNA double-strand breaks in cells. Furthermore, the present invention relates to products and applications based on this technology, which aims at detecting in a sample the existence of DNA double-strand breaks in cells, as well as quantification of DNA double-strand breaks.
PCT/GR2017/000061 2016-10-11 2017-10-10 Antibodies to a specific phosphorylation of u3kqk0 and its uses WO2018069741A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GR20160100524 2016-10-11
GR20160100524A GR1009241B (en) 2016-10-11 2016-10-11 Antibody against specific phosphorylation of the u3kqk0 protein and use of the same

Publications (2)

Publication Number Publication Date
WO2018069741A2 WO2018069741A2 (en) 2018-04-19
WO2018069741A3 true WO2018069741A3 (en) 2018-06-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GR2017/000061 WO2018069741A2 (en) 2016-10-11 2017-10-10 Antibodies to a specific phosphorylation of u3kqk0 and its uses

Country Status (2)

Country Link
GR (1) GR1009241B (en)
WO (1) WO2018069741A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001004158A1 (en) * 1999-07-12 2001-01-18 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Method for determining dna double-stranded breaks
WO2003014142A2 (en) * 2001-08-03 2003-02-20 University Of Virginia Patent Foundation Phosphorylated histone h2b as an apoptosis marker

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005028620A2 (en) * 2003-09-16 2005-03-31 The Rockefeller University Histone modifications as binary switches controlling gene expression
US20140134178A1 (en) * 2012-01-06 2014-05-15 H. Lee Moffit Cancer Center And Research Institute Phosphorylation of histones and uses thereof
US9732146B2 (en) * 2012-03-30 2017-08-15 The United States Of America As Represented By The Department Of Veterans Affairs Antibody-mediated transduction of heat shock proteins into living cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001004158A1 (en) * 1999-07-12 2001-01-18 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Method for determining dna double-stranded breaks
WO2003014142A2 (en) * 2001-08-03 2003-02-20 University Of Virginia Patent Foundation Phosphorylated histone h2b as an apoptosis marker

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
ANDY T. Y. LAU ET AL: "Phosphorylation of Histone H2B Serine 32 Is Linked to Cell Transformation", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 30, 29 July 2011 (2011-07-29), US, pages 26628 - 26637, XP055468292, ISSN: 0021-9258, DOI: 10.1074/jbc.M110.215590 *
CHENG-SHENG LEE ET AL: "Dynamics of yeast histone H2A and H2B phosphorylation in response to a double-strand break", NAT. STRUCT. MOL. BIOL., vol. 21, no. 1, 1 January 2014 (2014-01-01), New York, pages 103 - 109, XP055468041, ISSN: 1545-9993, DOI: 10.1038/nsmb.2737 *
CHENG-SHENG LEE ET AL: "Supplementary Information: Dynamics of yeast histone H2A and H2B phosphorylation in response to a double-strand break", NAT. STRUCT. MOL. BIOL., vol. 21, no. 1, 1 January 2014 (2014-01-01), New York, pages 103 - 109, XP055468678, ISSN: 1545-9993, DOI: 10.1038/nsmb.2737 *
CHEUNG W L ET AL: "Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase", CELL, CELL PRESS, AMSTERDAM, NL, vol. 113, no. 4, 16 May 2003 (2003-05-16), pages 507 - 517, XP002353259, ISSN: 0092-8674, DOI: 10.1016/S0092-8674(03)00355-6 *
ELINOR R. FOSTER ET AL: "Histone H2A phosphorylation in DNA double-strand break repair : H2A phosphorylation and DNA repair", FEBS JOURNAL, vol. 272, no. 13, 24 June 2005 (2005-06-24), GB, pages 3231 - 3240, XP055467982, ISSN: 1742-464X, DOI: 10.1111/j.1742-4658.2005.04741.x *
HAICO VAN ATTIKUM ET AL: "The histone code at DNA breaks: a guide to repair?", NAT REV MOL CELL BIOL., vol. 6, no. 10, 15 September 2005 (2005-09-15), pages 757 - 765, XP055468395, ISSN: 1471-0072, DOI: 10.1038/nrm1737 *
OSCAR FERNANDEZ-CAPETILLO ET AL: "Phosphorylation of Histone H2B at DNA Double-Strand Breaks", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 199, no. 12, 21 June 2004 (2004-06-21), US, pages 1671 - 1677, XP055468297, ISSN: 0022-1007, DOI: 10.1084/jem.20032247 *
ROGAKOU E P ET AL: "DNA DOUBLE-STRANDED BREAKS INDUCE HISTONE H2AX PHOSPHORYLATION ON SERINE 139", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 273, no. 10, 6 March 1998 (1998-03-06), pages 5858 - 5868, XP000960410, ISSN: 0021-9258, DOI: 10.1074/JBC.273.10.5858 *
ROGAKOU E P ET AL: "INITIATION OF DNA FRAGMENTATION DURING APOPTOSIS INDUCES PHOSPHORYLATION OF H2AX HISTONE AT SERINE 139", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 275, no. 13, 31 March 2000 (2000-03-31), pages 9390 - 9395, XP000960404, ISSN: 0021-9258, DOI: 10.1074/JBC.275.13.9390 *
ROGAKOU E P ET AL: "MEGABASE CHROMATIN DOMAINS INVOLVED IN DNA DOUBLE-STRAND BREAKS IN VIVO", THE JOURNAL OF CELL BIOLOGY : JCB, THE ROCKEFELLER UNIVERSITY PRESS, US, vol. 146, no. 5, 6 September 1999 (1999-09-06), pages 905 - 915, XP000960407, ISSN: 0021-9525, DOI: 10.1083/JCB.146.5.905 *
ROHINTON T KAMAKAKA ET AL: "REVIEW Histone variants: deviants?", 1 January 2005 (2005-01-01), XP055468392, Retrieved from the Internet <URL:http://genesdev.cshlp.org/content/19/3/295.full.pdf#page=1&view=FitH> [retrieved on 20180418] *
UNIPROTKB: "B4DR52_HUMAN", 23 August 2008 (2008-08-23), XP055468439, Retrieved from the Internet <URL:http://www.uniprot.org/uniprot/B4DR52.txt> [retrieved on 20180418] *
UNIPROTKB: "U3KQK0_HUMAN", 13 November 2013 (2013-11-13), XP055468451, Retrieved from the Internet <URL:http://www.uniprot.org/uniprot/U3KQK0.txt> [retrieved on 20180418] *
UPSTATE: "Antibodies for Phosphorylation & Beyond", INTERNET CITATION, June 2004 (2004-06-01), XP002334236, Retrieved from the Internet <URL:http://www.upstate.com/img/pdf/antibodies_phos.pdf> [retrieved on 20050630] *

Also Published As

Publication number Publication date
GR1009241B (en) 2018-03-06
WO2018069741A2 (en) 2018-04-19

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