WO2018056660A1

WO2018056660A1 - Method for extracting high content of curcumin from turmeric using emulsifier and ultrasonication

Info

Publication number: WO2018056660A1
Application number: PCT/KR2017/010199
Authority: WO
Inventors: 김준태; 이수비; 이봄내
Original assignee: 계명대학교 산학협력단
Priority date: 2016-09-26
Filing date: 2017-09-18
Publication date: 2018-03-29
Also published as: KR20180033747A

Abstract

The present invention relates to a method for extracting curcumin from turmeric by using water as a solvent and processing an emulsifier and ultrasonication. The method can replace the conventional curcumin extraction method using an organic solvent to eliminate the risk of residual organic solvent and extract a high content of curcumin. When lecithin used as an emulsifier in the present invention is treated with ultrasonication, a high content of curcumin can be extracted at a level similar to the extraction method using an organic solvent. In addition, Tween 80 or Tween 20 can extract a high content of curcumin at a level similar to the extraction method using an organic solvent by a single addition without ultrasonication, and can further increase the extraction content of curcumin when treated with ultrasonication.

Description

High Curcumin Extraction Method from Turmeric Using Emulsifier and Ultrasonic

The present invention relates to a high content curcumin extraction method from turmeric using an emulsifier and ultrasonic waves.

Curcumin is a natural pigment contained in curry, mustard, etc. as the main active ingredient of turmeric, and has been used as a folk remedy such as spices or inflammation and skin diseases since ancient times. Curcumin has a strong antioxidant function and structurally belongs to a phenolic antioxidant, the attention of the scientific community has been attracting attention as the anti-cancer effect of curcumin has recently been revealed. Curcumin has been shown to inhibit NF-kB, which is involved in cancer cell growth, block the expression of major proteins associated with esophageal cancer, and induce tumor cell death in many other cancers, including skin cancer. In addition, it has been reported to inhibit the accumulation of brain cell disruption protein, which causes Alzheimer's disease, to delay disease progression, and to inhibit the expression of interleukin-8 (IL-8), an inflammation-inducing protein. have. In addition, various functions of curcumin have been reported, such as blocking blood vessel formation efficiently at concentrations that are not toxic to other cells, that is, not having cytotoxicity, thereby preventing nutrients from being supplied to cancer cells and inducing death of cancer cells.

Despite the excellent biological activity of curcumin, curcumin has generally been extracted through solvent extraction because of its insolubility in water. Extraction methods using organic solvents such as ethanol or dimethyl sulfoxide (DMSO) have a problem that residual waste and organic solvent remain after extraction, and the extraction efficiency of curcumin is also low.

Therefore, the necessity of a new extraction method for extracting a high content of curcumin by replacing the conventional extraction method using an organic solvent and improving the extraction efficiency is required, and curcumin extracted without using an organic solvent can be safely used as a food additive. Thus, consumers may be able to improve their perception of curcumin as a food additive.

It is an object of the present invention to provide a high content curcumin extraction method from turmeric using an emulsifier and ultrasonic waves.

In order to achieve the above object, the present invention comprises the first step of preparing a turmeric solution by dissolving turmeric powder in water; A second step of preparing a mixture by adding an emulsifier to the turmeric solution; And a third step of extracting curcumin from the mixture.

In addition, the present invention comprises the first step of preparing a turmeric solution by dissolving turmeric powder in water; A second step of preparing a mixture by adding 0.5-5% (w / v) lecithin to the turmeric solution; A third step of treating the mixture with an ultrasonic wave at an amplitude of 20% to 80% for 1 minute to 20 minutes; And a fourth step of extracting curcumin from the mixture treated with the ultrasonic waves.

In addition, the present invention comprises the first step of preparing a turmeric solution by dissolving turmeric powder in water; A second step of preparing a mixture by adding 0.5 to 5% (w / v) of Tween 80 or Tween 20 to the turmeric solution; A third step of treating the mixture with an ultrasonic wave at an amplitude of 20% to 80% for 1 minute to 20 minutes; And a fourth step of extracting curcumin from the mixture treated with the ultrasonic waves.

The present invention is a method of extracting curcumin from turmeric by using water as a solvent and by treating with emulsifier and ultrasonic (ultrasonication), the method replaces the conventional curcumin extraction method using an organic solvent to remove the risk of residual organic solvent, A high content of curcumin can be extracted.

The lecithin used as the emulsifier of the present invention, when treated with ultrasound, can extract a high content of curcumin at a level similar to that extracted using an organic solvent. In addition, Tween 80 or Tween 20 may extract high content curcumin to a level similar to the extraction method using an organic solvent by addition alone without ultrasonication, and may further increase the curcumin extraction content when treated with ultrasound.

Figure 1 compares the curcumin extraction content according to the solvent by spectrophotometric method.

2 is a spectrophotometric comparison of curcumin extraction content with 2% lecithin addition and sonication time.

3 is a spectrophotometric comparison of curcumin extract content with lecithin content and sonication time.

4 and 5 compare the curcumin extraction content with 2% Tween addition and sonication time by spectrophotometric method.

6 and 7 compare the curcumin extraction content according to the twin content and the ultrasonic treatment time by spectrophotometry.

8 is a spectrophotometric comparison of curcumin extraction content according to the Tween 80 content.

Figure 9 is a spectrophotometric comparison of the curcumin extraction content according to the emulsifier type, emulsifier content and sonication time.

In the present invention, the inventors have developed a method of extracting curcumin from turmeric by using water as a solvent and treating emulsifiers and ultrasonics, and the method replaces the conventional curcumin extraction method using organic solvents, and thus risks of residual organic solvents. Removed, confirmed that the high content of curcumin to extract and completed the present invention.

The term "curcumin" as used in the present invention is the main active ingredient of turmeric and is used as a food additive as a kind of alkaloid or curcuminoid. Curcumin has a strong antioxidant function and recent studies have shown anti-inflammatory or anti-cancer effects.

As used herein, the term “emulsifier or surfactant” is a substance having both hydrophilic and lipophilic functional groups, and serves to help dissolve or disperse components that are insoluble in water.

The term “ultrasonication” used in the present invention is a physical treatment method used to extract a functional substance, which delivers high energy, destroys cells, and has an effect of separating functional substances contained in cells.

As used herein, the term “lecithin” is a term referring to a group of yellowish fatty substances that occur in animal and plant tissues composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides, and phospholipids. Lecithin can be separated chemically or mechanically and is usually isolated from soybeans, eggs, milk, seafood, rapeseed, cottonseed, sunflower, and the like. Lecithin has a low water solubility and is used as an emulsifier.

As used herein, the term “tween” is also called polysorbate and is an emulsifier used in the manufacture of some pharmaceuticals and foods. Tweens are also often used in cosmetics that dissolve essential oils in water-based products.

The present invention comprises a first step of preparing a turmeric solution by dissolving turmeric powder in water; A second step of preparing a mixture by adding an emulsifier to the turmeric solution; And a third step of extracting curcumin from the mixture.

Preferably, after the second step, the step of treating the ultrasonic wave to the mixture; may further include.

Preferably, the emulsifier may be selected from the group consisting of lecithin, tween 80 and tween 20, but is not limited thereto.

Preferably, the lecithin may be contained in 0.5 to 5% (w / v).

Preferably, the tween 80 or tween 20 may be contained in 0.5 to 5% (w / v).

Preferably, the ultrasonic waves may be treated for 1 minute to 20 minutes at 20% to 80% of amplitude.

The type, content, and sonication condition of the emulsifier are not limited to the above ranges, but when it is out of the above ranges, curcumin extraction efficiency may be lowered, which may be undesirable to extract high content of curcumin.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

실시예 1 : 용매에 따른 커큐민 추출 함량Example 1 Curcumin Extraction Content According to Solvents

Turmeric powder was added at a concentration of 1% (w / v) using 30 mL of water, 50% ethanol or 100% dimethyl sulfoxide (DMSO) as a solvent, and extracted at room temperature for 24 hours. Curcumin content extracted according to each solvent was measured by absorbance by curcumin at 425 nm wavelength using UV-Vis spectrophotometry (UV-2600, Shimadzu, Japan). Curcumin (purity 98.0% or more), a standard reagent purchased from Sigma-Aldrich, was used to create a standard curve, and the curcumin content extracted according to the extraction solvent was calculated by substituting the absorbance values of each extract. It was. Curcumin content was measured at least three times and expressed as an average value.

As a result, referring to Figure 1, it was confirmed that the curcumin containing 4.29 μg / mL in water (DW) extract, 440.6 μg / mL in 50% ethanol extract, and 517.6 μg / mL in dimethyl sulfoxide extract. The amount of curcumin extracted using pure water as a solvent was about 4.29 μg / mL, but the amount of curcumin extracted using 50% ethanol or dimethyl sulfoxide as a solvent was 440.6 μg / mL and 517.6 μg / ml, respectively. As a result, it increased by about 100 to 120 times.

실시예Example 2 : 2%2: 2% 레시틴(lecithin) 첨가 및 초음파 처리시간에 따른 Depending on lecithin addition and sonication time 커큐민Curcumin 추출 함량 Extraction content

Turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v) and mixed with 2% soy lecithin. Thereafter, ultrasonic waves were treated for 0, 1, 3, 5, 10 and 20 minutes, and curcumin was extracted at room temperature for 24 hours to compare the curcumin extraction content according to lecithin addition and sonication time. The ultrasonic probe used Vibra cell VCX-750 (Sonics & Materials, Inc., USA), power 20 kHz, amplitude 40%, pulse on / off mode 3s / 2s was set.

As a result, FIG. 2 compares the curcumin extraction content according to the ultrasonic treatment time using water containing 2% soybean lecithin, and about 48.8 μg / mL of curcumin was extracted under the ultrasonic treatment. It was confirmed that the extraction content of curcumin gradually increased with increasing treatment time. In addition, a curcumin of 388.2 μg / mL, which was the maximum value when the sonication time was 10 minutes, was extracted. This was confirmed that the extraction content similar to the extraction method using 50% ethanol as a solvent.

실시예 3 : 레시틴 함량 및 초음파 처리시간에 따른 커큐민 추출 함량Example 3 Curcumin Extraction Content According to Lecithin Content and Ultrasonic Treatment Time

Turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v), and the contents of soy lecithin were added at 0, 1, 2% and mixed. Thereafter, ultrasonic treatment was performed for 0, 1, 3, 5, and 10 minutes for each condition, and curcumin was extracted for 24 hours at room temperature, and the lecithin content and curcumin extraction content according to the ultrasonic treatment time were compared.

As a result, referring to Figure 3, a very small amount of curcumin was extracted from the water extract (no-surfactant) without the addition of lecithin 4.29 μg / mL, even if the ultrasonic treatment time up to 10 minutes 32.12 μg Although increased to / mL, the curcumin content was very low compared to the extraction method using an organic solvent.

However, when 1% or 2% of lecithin was added to the solvent, the curcumin content was 29.4 μg / mL and 48.8 μg / mL, respectively, when the sonication time was 0 minutes, which is higher than the extraction method using pure water. Indicated. In addition, it was confirmed that the curcumin content of about 90.5 times increased to 242.4 μg / mL in 1% lecithin and 388.2 μg / mL in 2% lecithin when sonicated for 10 minutes.

실시예Example 4 : 2%4: 2% 트윈Twin 80(Tween 80) 또는 2% 80 (Tween 80) or 2% 트윈Twin 20(Tween 20) 첨가, 및 초음파 처리시간에 따른 커큐민 추출 함량 Curcumin Extraction Content with Tween 20 Addition and Ultrasonication Time

Instead of soybean lecithin, curcumin was extracted using TLB 80 and Tween 20 with hydrophile lipophile balance (HLB) values of 15.0 and 16.7. HLB represents a numerical value based on the balance of hydrophobic and hydrophilic groups.

Turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v) and mixed by addition of 2% Tween 80 or Tween 20. Thereafter, ultrasonic waves were treated for 0, 1, 3, 5, 10, 20 minutes and curcumin was extracted at room temperature for 24 hours, and the curcumin extraction contents were compared according to the twin addition and the ultrasonic treatment time.

As a result, referring to Figures 4 and 5, the addition of 2% Tween 80 or Tween 20 showed an extraction content similar to the method extracted using the organic solvent even in the conditions without ultrasonic treatment. In addition, additional sonication did not show a significant effect on curcumin extraction content over time.

실시예 5 : 트윈 함량 및 초음파 처리시간에 따른 커큐민 추출 함량Example 5 Curcumin Extraction Content According to Tween Content and Ultrasonic Treatment Time

Turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v), and the contents of Tween 80 or Tween 20 were added at 0, 1, 2% and mixed. Thereafter, the ultrasonic wave was treated for 0, 1, 3, 5, and 10 minutes for each condition, and curcumin was extracted at room temperature for 24 hours, and the content of tween and curcumin extract according to the ultrasonic treatment time were compared.

As a result, referring to Figure 6, when the 1% of Tween 80 was added compared to the water extract without the Tween 80, the curcumin content increased by about 71.44 times to 306.47 μg / mL, and when the ultrasonic treatment for 3 minutes Curcumin was extracted to the maximum value at 434.12 μg / mL, which showed an extraction content similar to that extracted using 50% ethanol as a solvent. However, when 2% of Tween 80 was added, the effect of sonication time could not be observed.

Referring to FIG. 7, the addition of Tween 20 showed a similar tendency to the addition of Tween 80. When 1% of Tween 20 was added, curcumin extraction content tended to increase after more than 1 minute of sonication, and the effect of sonication time was observed when 2% of Tween 20 was added. Could not.

In addition, it was confirmed that the curcumin extract content slightly higher when the Tween 80 was added than the Tween 20 was added.

실시예 6 : 트윈 80 함량에 따른 커큐민 추출 함량Example 6 Curcumin Extraction Content According to Tween 80 Content

Turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v) and Tween 80 was mixed up to 4%. Thereafter, curcumin was extracted for 24 hours at room temperature without sonication, and the curcumin extraction content according to the Tween 80 content was compared, and the control was extracted using 50% ethanol solvent.

As a result, referring to Figure 8, when the content of Tween 80 increased from 1% to 3%, the curcumin extraction content tended to increase little by little. It was confirmed that not. In addition, when 3% or more of Tween 80 was added, the extraction content was similar to that extracted using 50% ethanol as a solvent.

실시예Example 7 : 유화제 종류, 유화제 함량 및 초음파 처리시간에 따른 7: according to the type of emulsifier, the amount of emulsifier and the ultrasonic treatment time 커큐민Curcumin 추출 함량 Extraction content

To compare the results according to Examples 2 to 6 above, turmeric powder is added to 30 mL of pure water at a concentration of 1% (w / v), and lecithin, Tween 80, Tween 20 as emulsifier 1% Or 2% added and mixed. Thereafter, ultrasonic waves were treated for 0 and 10 minutes and curcumin was extracted at room temperature for 24 hours to compare the curcumin contents.

As a result, referring to Figure 9, the extraction content of curcumin was increased after the ultrasonic treatment under the condition that the emulsifier is not added, but a high content of curcumin was not extracted. However, when 1% of Tween 80 or Tween 20 was added, the curcumin extraction content increased after sonication. When 2% of Tween 80 or Tween 20 was added, the effect of treatment time was not observed. However, in the case of adding 1% or 2% of the lecithin, the curcumin content of 7 to 10 times or more was confirmed after sonication.

As described above in detail a specific part of the present invention, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

The scope of the present invention is represented by the following claims, and it should be construed that all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the present invention.

Claims

Dissolving turmeric powder in water to prepare a turmeric solution;

A second step of preparing a mixture by adding an emulsifier to the turmeric solution; And

Extracting curcumin from the mixture;

Curcumin extraction method comprising a.
According to claim 1, After the second step, further comprising the step of ultrasonically treating the mixture; curcumin extraction method further comprising.
The method of claim 1, wherein the emulsifier is selected from the group consisting of lecithin, tween 80, and tween 20.
The method of claim 3, wherein the lecithin is contained in 0.5 to 5% (w / v), curcumin extraction method.
The method of claim 3, wherein the Tween 80 or Tween 20 is contained at 0.5 to 5% (w / v).
The method of claim 2, wherein the ultrasonic wave is treated for 1 minute to 20 minutes at an amplitude of 20% to 80%.
The method of claim 2, wherein the extraction method

Dissolving turmeric powder in water to prepare a turmeric solution;

A second step of preparing a mixture by adding 0.5-5% (w / v) lecithin to the turmeric solution;

A third step of treating the mixture with an ultrasonic wave at an amplitude of 20% to 80% for 1 minute to 20 minutes; And

A fourth step of extracting curcumin from the ultrasonically treated mixture;

Curcumin extraction method comprising a.
The method of claim 2, wherein the extraction method

Dissolving turmeric powder in water to prepare a turmeric solution;

A second step of preparing a mixture by adding 0.5 to 5% (w / v) of Tween 80 or Tween 20 to the turmeric solution;

A third step of treating the mixture with an ultrasonic wave at an amplitude of 20% to 80% for 1 minute to 20 minutes; And

A fourth step of extracting curcumin from the ultrasonically treated mixture;

Curcumin extraction method comprising a.