KR20180083285A - Extraction method of high content of curcumin from Turmeric using emulsifiers and ultrasonication - Google Patents

Abstract

The present invention relates to a method for extracting curcumin from turmeric using water as a solvent and processing an emulsifier and ultrasonication, which removes the risk of residual organic solvents and can extract a high content of curcumin by replacing a conventional method for extracting curcumin using an organic solvent. When lecithin used as an emulsifier of the present invention is treated with ultrasonic waves, a high content of curcumin can be extracted at a similar level to an extracting method using an organic solvent. In addition, Tween 80 or 20 can extract a high content of curcumin at a similar level to the extracting method using an organic solvent by single addition even without ultrasonication, and can further increase the extraction content of curcumin when being treated with ultrasonic waves.

Description

[0002] Extraction methods of curcumin from turmeric using emulsifiers and ultrasonic waves [0003]

The present invention relates to a method for extracting a high content of curcumin from turmeric using an emulsifier and an ultrasonic wave.

Curcumin is a natural coloring matter contained in curry, mustard, etc. as a main active ingredient of turmeric, and has been used as a curing agent for spices or inflammation and skin diseases since ancient times. Curcumin has strong antioxidant properties and is structurally related to phenolic antioxidants. Recently, curcumin has been shown to have an anticancer effect. Curcumine inhibits NF-kB, which is involved in cancer cell growth, to block the expression of key proteins involved in esophageal cancer, and has been shown to induce the death of tumor cells in various other cancers such as skin cancer. In addition, it inhibited the accumulation of brain-destroying protein, which is the cause of Alzheimer's disease, and delayed the progression of the disease, and suppressed the expression of inflammatory protein, interleukin-8 (IL-8). In addition, various functions of curcumin have been reported, such as inhibiting the supply of nutrients to the cancer by effectively inhibiting angiogenesis at concentrations that do not show toxicity to other cells, that is, not cytotoxic, and induce the death of cancer cells.

Despite the excellent biological activity of curcumin, curcumin is generally insoluble and has very low solubility in water and has therefore been extracted via solvent extraction. The extraction method using an organic solvent such as ethanol or dimethyl sulfoxide (DMSO) has a problem that the residual waste and the organic solvent remain after extraction, and the extraction efficiency of curcumin is also low.

Therefore, there is a need for a new extraction method for extracting a high content of curcumin by replacing an extraction method using an organic solvent and improving the extraction efficiency, and curcumin extracted without using an organic solvent can be safely used as a food additive To improve the perception of curcumin as a food additive for consumers.

Korean Registered Patent No. 10-1582306 (Registered on December 28, 2015)

The object of the present invention is to provide a process for producing a cured product which comprises a first step of dissolving a sulfur powder in water, a second step of adding an emulsifier to the dissolved sulfuric acid solution and reacting, and a third step of extracting curcumin from the reacted solution The present invention provides a method of extracting curcumin.

A further object of the present invention is to provide a method for preparing a fermentation product which comprises a first step of dissolving a sulfur powder in water, a second step of adding 0.5 to 5% (w / v) lecithin to the dissolved sulfuric acid solution and reacting, A third step of processing at an amplitude of 20% to 80% for 1 minute to 20 minutes, and a fourth step of extracting curcumin from the solution treated with the ultrasonic wave. .

Another object of the present invention is to provide a method for producing a silver halide emulsion which comprises a first step of dissolving a sulfur powder in water, a second step of adding 0.5 to 5% (w / v) of Tween 80 or Tween 20 to the dissolved sulfuric acid solution, A third step of treating ultrasonic waves with an amplitude of 20% to 80% for 1 minute to 20 minutes, and a fourth step of extracting curcumin from the solution treated with ultrasonic waves. And to provide an extraction method.

In order to attain the above object, the present invention provides a method for producing a fermentation product, comprising: a first step of dissolving a sulfurized powder in water; a second step of adding an emulsifier to the dissolved sulfuric acid solution and reacting; and a third step of extracting curcumin from the reacted solution The present invention also provides a method for extracting curcumin.

In addition, the present invention provides a method for preparing a fermentation product, comprising the steps of: a step of dissolving a sulfurized powder in water; a step of adding 0.5 to 5% (w / v) of lecithin to the dissolved sulfuric acid solution followed by a reaction; and a fourth step of extracting curcumin from the solution treated with the ultrasonic wave. The present invention also provides a method for extracting curcumin, comprising the steps of: (1) treating the solution with an amplitude of 20% to 80% for 1 minute to 20 minutes;

The present invention also provides a method for producing a silver halide emulsion, comprising a first step of dissolving a sulfur powder in water, a second step of adding 0.5 to 5% (w / v) of Tween 80 or Tween 20 to the dissolved sulfuric acid solution, A third step of treating the ultrasonic waves at an amplitude of 20% to 80% for 1 minute to 20 minutes, and a fourth step of extracting curcumin from the solution treated with the ultrasonic waves. to provide.

The present invention relates to a method for extracting curcumin from turmeric by using water as a solvent and an emulsifier and ultrasonication. This method replaces the curcumin extraction method using a conventional organic solvent to eliminate the risk of residual organic solvent, A high content of curcumin can be extracted.

When the lecithin used as the emulsifier of the present invention is treated with ultrasonic waves, a high content of curcumin can be extracted at a level similar to that obtained by using an organic solvent. In addition, tween 80 or tween 20 can extract high-content curcumin at a level similar to the extraction method using an organic solvent by singly adding without ultrasonic treatment, and can further increase the content of curcumin extract when treated with ultrasonic waves.

Fig. 1 is a spectrophotometric comparison of the content of curcumin extracted according to the solvent.
FIG. 2 is a spectrophotometric comparison of the content of curcumin extracted with 2% lecithin addition and sonication time.
FIG. 3 is a spectrophotometric comparison of the content of curcumin extracted according to lecithin content and sonication time.
FIGS. 4 and 5 are spectrophotometric comparisons of the content of curcumin extracted with 2% tween addition and sonication time.
FIGS. 6 and 7 are spectrophotometric comparisons of the content of curcumin extracted according to the twin content and the sonication time.
8 is a spectrophotometric comparison of curcumin extract content according to the tween 80 content.
Fig. 9 is a spectrophotometric comparison of the content of curcumin extracted with the kind of emulsifier, the content of emulsifier and the time of ultrasonic treatment.

In the present invention, the inventors of the present invention have developed a method of extracting curcumin from turmeric by using water as a solvent, emulsifying agent and ultrasonication, and this method has been developed to replace the curcumin extraction method using an organic solvent, And a high content of curcumin was extracted. Thus, the present invention was completed.

As used herein, the term " curcumin " is the main active ingredient of turmeric and is used as a food additive as an alkaloid or a kind of cucuminoid. Curcumin has strong antioxidant properties and has recently been shown to have anti-inflammatory and anti-cancer effects.

The term " emulsifier or surfactant " used in the present invention is a substance having both hydrophilic and lipophilic functional groups, and functions to help water-insoluble components to be dissolved or dispersed in water.

The term " ultrasonication " used in the present invention is a physical treatment method used for extracting a functional substance, and has an effect of separating a functional substance contained in a cell by delivering high energy to destroy the cell.

The term " lecithin " as used in the present invention is a term referring to a group of fatty substances of yellowish-purple color occurring in plant and animal tissues composed of phosphoric acid, choline, fatty acid, glycerol, glycolipid, triglyceride and phospholipid. Lecithin can be separated chemically or mechanically and can generally be separated from beans, eggs, milk, seafood, oilseed rape, cottonseed, sunflower, and the like. Lecithin has low water solubility and is used as an emulsifier.

The term " tween ", as used herein, is also referred to as polysorbate and is an emulsifier used in the manufacture of certain medicines and foods. Tweens are also used in cosmetics that often dissolve essential oils in water-based products.

The present invention relates to a method for producing curcumin, which comprises a first step of dissolving a sulfur powder in water, a second step of adding an emulsifier to the dissolved sulfuric acid solution and reacting, and a third step of extracting curcumin from the reacted solution. The extraction method is provided.

The method may further include the step of treating ultrasonic waves in the solution between the second step and the third step.

Preferably, the emulsifier may be selected from the group consisting of lecithin, tween 80 and tween 20, but is not limited thereto.

Preferably, the lecithin may be contained in an amount of 0.5 to 5% (w / v).

Preferably, the tween 80 or tween 20 may be contained in an amount of 0.5 to 5% (w / v).

Preferably, the ultrasonic waves can be processed at an amplitude of 20% to 80% for 1 minute to 20 minutes.

If the content of the emulsifier and the condition of the ultrasonic treatment are out of the above ranges, the extraction efficiency of curcumin may be lowered and it may not be preferable to extract a high content of curcumin.

In addition, the present invention provides a method for preparing a fermentation product, comprising the steps of: a step of dissolving a sulfurized powder in water; a step of adding 0.5 to 5% (w / v) of lecithin to the dissolved sulfuric acid solution followed by a reaction; and a fourth step of extracting curcumin from the solution treated with the ultrasonic wave. The present invention also provides a method for extracting curcumin, comprising the steps of: (1) treating the solution with an amplitude of 20% to 80% for 1 minute to 20 minutes;

The present invention also provides a method for producing a silver halide emulsion, comprising a first step of dissolving a sulfur powder in water, a second step of adding 0.5 to 5% (w / v) of Tween 80 or Tween 20 to the dissolved sulfuric acid solution, A third step of treating the ultrasonic waves at an amplitude of 20% to 80% for 1 minute to 20 minutes, and a fourth step of extracting curcumin from the solution treated with the ultrasonic waves. to provide.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example  1: depending on the solvent Curcumin  Extract content

The sulfuric acid powder was added at a concentration of 1% (w / v) using 30 mL of water, 50% ethanol or 100% dimethyl sulfoxide (DMSO) as a solvent and extracted at room temperature for 24 hours. The absorbance of curcumin extracted at various wavelengths was measured by UV-Vis spectrophotometer (UV-2600, Shimadzu, Japan) at a wavelength of 425 nm. Curcumin, a standard reagent purchased from Sigma-Aldrich, having a purity of 98.0% or more, was used to prepare a standard curve, and the absorbance value of each extract was substituted to calculate the amount of curcumin extracted according to the extraction solvent Respectively. Measurements of curcumin content were repeated at least three times and expressed as mean values.

As a result, it was confirmed that the water (DW) extract contained curcumin at 4.29 μg / mL, 50% ethanol extract at 440.6 μg / mL, and dimethylsulfoxide extract at 517.6 μg / mL. The content of curcumin extracted with pure water as a solvent was very low at about 4.29 μg / mL, but the content of curcumin extracted with 50% ethanol or dimethyl sulfoxide as a solvent was 440.6 μg / mL and 517.6 μg / mL, respectively To about 100 times to 120 times.

Example 2: 2%  Lecithin addition and ultrasonic treatment time Curcumin  Extract content

The turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v), and 2% soy lecithin was added and mixed. After that, ultrasonic waves were treated for 0, 1, 3, 5, 10, and 20 minutes, and curcumin was extracted at room temperature for 24 hours to compare curcumin extract contents with lecithin addition and ultrasonic treatment time. The ultrasonic probe was a Vibra cell VCX-750 (Sonics & Materials, Inc., USA), with a power of 20 kHz, an amplitude of 40% and a pulse on / off mode 3s / 2s.

As a result, FIG. 2 shows that curcumin extracted at a concentration of about 48.8 μg / mL was used under the condition not treated with ultrasound, while ultrasonic treatment with water containing 2% soybean lecithin was used as a solvent. As the treatment time increased, the content of curcumin increased gradually. In addition, when the ultrasonic treatment time was 10 minutes, the maximum value of 388.2 μg / mL of curcumin was extracted. It was confirmed that the extract contents were similar to those obtained by using 50% ethanol as a solvent.

Example  3: Depending on lecithin content and ultrasonic treatment time Curcumin  Extract content

Turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v), and the content of soybean lecithin was added at 0, 1, 2% and mixed. After that, ultrasonic waves were treated for 0, 1, 3, 5, and 10 minutes for each condition, and curcumin was extracted at room temperature for 24 hours to compare curcumin extract contents with lecithin content and ultrasonic treatment time.

As a result, referring to FIG. 3, a very small amount of curcumin was extracted at 4.29 μg / mL in no-surfactant without lecithin, and even when the ultrasonic treatment time was 10 minutes, 32.12 μg / mL, but the content of curcumin was very low as compared with the extraction method using an organic solvent.

However, when 1% or 2% of lecithin was added to the solvent, the content of curcumin was 29.4 μg / mL and 48.8 μg / mL, respectively, when the ultrasonic treatment time was 0 minutes, Respectively. In addition, it was confirmed that the content of curcumin increased by 90.5 times to 242.4 μg / mL for 1% lecithin and 388.2 μg / mL for 2% lecithin when ultrasonic treatment was performed for 10 minutes.

Example 4: 2% Twin  80 (Tween 80) or 2% Twin  20 (Tween 20), and by ultrasonic treatment time Curcumin  Extract content

Curcumin was extracted by using Tween 80 and Tween 20, which have HLB (hydrophile lipophile balance) values of 15.0 and 16.7, as emulsifiers instead of soybean lecithin. The HLB represents a numerical value based on the balance between hydrophobic and hydrophilic groups.

The turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v), and 2% Tween 80 or Tween 20 was added and mixed. Then, ultrasonic waves were treated for 0, 1, 3, 5, 10, and 20 minutes, and curcumin was extracted at room temperature for 24 hours to compare curcumin extract contents with twin addition and sonication time.

As a result, referring to FIG. 4 and FIG. 5, when 2% Tween 80 or Tween 20 was added, extraction contents similar to those obtained by extraction using an organic solvent were shown even under the condition of no ultrasonic treatment. In addition, additional sonication did not significantly affect the curcumin extract content over time.

Example  5: Twin  Depending on the content and ultrasonic treatment time Curcumin  Extract content

The turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v), and the content of Tween 80 or Tween 20 was added at 0, 1, 2% and mixed. After that, ultrasonic waves were treated for 0, 1, 3, 5, and 10 minutes for each condition, and curcumin was extracted at room temperature for 24 hours to compare curcumin extract contents with respect to the twin content and the ultrasonic treatment time.

As a result, referring to FIG. 6, the content of curcumin increased by about 71.44 times to 306.47 μg / mL when 1% of tween 80 was added compared to the water extract without addition of tween 80, and when ultrasonic waves were treated for 3 minutes Curcumin was extracted at a maximum of 434.12 μg / mL, which was similar to that extracted with 50% ethanol as a solvent. However, when 2% of Tween 80 was added, the effect according to the ultrasonic treatment time could not be observed.

Referring to FIG. 7, the addition of Tween 20 showed a tendency similar to that of Tween 80. When 1% Tween 20 was added, the content of curcumin increased after 1 minute of ultrasonic treatment compared to the condition without ultrasonic treatment. When 2% Tween 20 was added, the effect of ultrasonic treatment time was observed I could not.

Also, it was confirmed that the content of extract of curcumin was slightly higher when Tween 80 was added than that of Tween 20.

Example  6: Twin  80 content Curcumin  Extract content

The turmeric powder was added to 30 mL of pure water at a concentration of 1% (w / v) and the tween 80 was mixed up to a maximum of 4%. Then, curcumin was extracted for 24 hours at room temperature without ultrasonic treatment to compare curcumin extract contents according to Tween 80 content, and extracted with a 50% ethanol solvent as a control.

As a result, referring to FIG. 8, when the content of tween 80 was increased from 1% to 3%, the extract content of curcumin tended to increase little, but when the content of tween 80 was more than 4%, the content of curcumin extract increased . In case of addition of more than 3% of Tween 80, extraction contents similar to the extraction method using 50% ethanol as a solvent were shown.

Example  7: Depending on emulsifier type, emulsifier content and ultrasonic treatment time Curcumin  Extract content

In order to compare the results according to Examples 2 to 6 with each other, the powder of the sulfuric acid was added to 30 mL of pure water at a concentration of 1% (w / v), lecithin, Tween 80, Or 2%. Thereafter, ultrasonic waves were treated for 0, 10 minutes, and curcumin was extracted at room temperature for 24 hours to compare curcumin contents.

As a result, referring to FIG. 9, the extract content of curcumin increased after ultrasonication under the condition that no emulsifier was added, but a high content of curcumin was not extracted. However, when 1% Tween 80 or Tween 20 was added, the amount of curcumin extracted after the ultrasonic treatment was greatly increased. When 2% was added, the effect of treatment time was not observed. However, in case of lecithin, the content of curcumin increased by 7 to 10 times or more after ultrasonication in all cases where 1% or 2% was added.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (1)

A first step of dissolving the sulfur powder in water;
A second step of adding 1 to 2% (w / v) lecithin to the dissolved sulfuric acid solution and reacting;
A third step of treating the reacted solution with ultrasonic waves at a frequency of 20 kHz for 1 minute to 20 minutes; And
A fourth step of extracting curcumin from the solution treated with the ultrasonic wave;
And extracting the curcumin.

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