WO2018048025A1

WO2018048025A1 - Pattern structure of diagnostic sensor

Info

Publication number: WO2018048025A1
Application number: PCT/KR2016/013698
Authority: WO
Inventors: 김민곤; 송문범; 변주영; 홍동구; 정권영
Original assignee: 주식회사 인지바이오
Priority date: 2016-09-07
Filing date: 2016-11-25
Publication date: 2018-03-15
Also published as: KR20180027913A; KR101867823B1

Abstract

The present invention relates to a pattern structure of a diagnostic sensor, wherein the pattern is formed by printing an organic mixture on paper. More particularly, the present invention relates to a pattern structure of a diagnostic sensor, wherein the pattern structure enables detection and diagnosis to be performed by transferring two kinds of materials at different times so that the two materials react without interference from each other. For example, a first material and a second material can be transferred to a diagnosis area at different times by different fluid flows, and reaction of the second material can be induced in the diagnosis area without interference from the first material that was supplied earlier.

Description

Specification

Name of invention: Pattern structure technology of diagnostic sensor

[1] The present invention relates to a diagnostic sensor for printing an organic compound on paper to form a pattern.

With regard to the pattern structure, more specifically, the first material and the second material are transferred to the diagnosis area in time by different fluid flows, and in the diagnosis area, the reaction of the second material is induced without disturbing the first supplied material. It is about the pattern structure of the diagnostic sensor that delivers two kinds of materials by time difference and returns them without any repayment.

Background

[2] Recent advances in medical and medical engineering have led to the introduction of various causes and treatments, as well as more accurate diagnosis of various diseases and the development of more effective and safe treatments. The rate of treatment is increasing.

[3] Nevertheless, the diagnosis and treatment of these diseases is mainly carried out in hospitals.

In the case of a diagnosis subject who wants to be diagnosed, there was a problem that the diagnosis and diagnosis of the disease could not be avoided without visiting the hospital directly. Therefore, expensive examination and treatment costs for the diagnosis subject or patient It was a total burden.

[4] Recently, due to the development of the electronics industry and wired and wireless communication networks,

As part of our efforts to solve problems and difficulties, the Telemedicin system has been introduced, which allows a patient or patient to be diagnosed at a remote location at a relatively low cost without visiting a hospital. However, until now, most of the external diagnosis has been made, and the diagnosis method can only be very limited, which makes it difficult to make an accurate diagnosis.

[5] In particular, diseases that may cause problems such as life threats (eg, various cancers, AIDS, etc.) are usually infected and developed by antigens such as pathogens, which require prompt diagnosis. F. It is urgent to develop a device capable of diagnosing biomaterials including various antigens.

[6] A biosensor using membrane is used as an immunoassay device that is simple to use, short in execution time, without requiring specialized knowledge or complicated procedures. ^`` Analytical devices using these biosensors can generally use an immunochromatographic method that uses a porous membrane as the immobilization matrix for the protein.As the sample containing the analyte is absorbed from the bottom of the membrane strip, capillary The analyte is then immobilized into The transport and reaction between the antigen and the antibody on the solid surface and the unbound components are separated by the fluid flow. This principle-based membrane strip immunochromatography technique uses the lateral flow of the fluid to react the reaction components. By accelerating the delivery of these substances, diagnostics can be completed simply by adding the analyte to the measurement speed and adding the sample.

[7] Recently, these biosensors use wax, paraffin, etc. on the membrane to feed channels.

Although research is being conducted on printing, it has a problem that the reliability of detection decreases due to the influence of temperature and pressure and the flow of fluid when forming multiple patterns on the membrane.

[8] Korean Patent Laid-Open No. 2002-0078032 (published Oct. 18, 2002; hereafter referred to as Priority Document 1).

The non-pregnancy diagnosis kit and the non-pregnancy diagnosis method of an animal using the same are presented in the above-mentioned document. conjugate) and the other end of the

Assay strips immobilized with immunoglobulin (IgG); and antiprogesterone

The immunoglobulin-gold label conjugate (Anti-progesterone IgG-gold conjugate) was made. Diagnosis is achieved by passing reactions through the serum albumin conjugate diagnostic line, which results in color development, or by mixing various reaction substances in the sample to make the diagnosis response on the diagnosis line. Diagnostic errors may occur due to low or non-specific reactions.

[9] Korean Patent Registration No. 10-1412777 (published June 20, 2014; hereafter referred to as Priority Document 2)

The lateral flow device for multi-component simultaneous quantitative analysis is presented. The lateral flow device of the prior document 2 includes: a sample pad for receiving a fluid sample to be analyzed; and placed in contact with the sample pad. Coated with a first binder that binds to different target analytes contained within the fluid sample.

A conjugate pad comprising nanospheres; and a patterned sheet disposed in contact with the conjugate pad, the patterned sheet having a branched channel pattern on which the fluid sample can flow, wherein the channel pattern includes: The pattern material is formed by spraying a pattern material on the base member of silver paper using inkjet printing. The above-mentioned document 2 shows a channel in which a channel pattern is branched from one channel to three channels. Fluid sample into one channel. As the process progresses, it is divided into several parts, and each branched channel contains different reaction materials so that multiple diagnosis can be performed at one time.As a result, the detection sensitivity is lowered or non-specific reactions are caused by the interference between materials. The disadvantage that occurred was not solved. Detailed description of the invention

Technical task

[10] The pattern structure of the diagnostic sensor of the present invention is

[11] In the diagnostic sensor field, which is a biosensor for printing organic materials on paper, a sample containing a target material delivered to a detection area by detection, or a plurality of reaction materials including a signal amplification material can be delivered over time. Therefore, the object of the present invention is to provide a pattern structure that can improve detection sensitivity by allowing each reaction to be performed in a substantial manner without interference from other materials.

Task solution

[12] The pattern structure of the diagnostic sensor of the present invention for achieving the above object is

[13] A pattern structure of a diagnostic sensor in which a channel is formed using a printing technique on paper, which is formed to be long in the vertical direction so that fluid flows from the lower part to the upper part. A main channel having a diagnostic region coated with a detection material at an upper end thereof, and having an intersecting area connected to another channel at a middle portion thereof; and a main channel formed side by side on the main channel; A buffer input area into which a buffer is inserted is separated from the area below the delayed fluidized area, and the buffer input area is connected to each other by a delay pad, so that the time delay for transferring the buffer to the main channel is delayed. A delayed channel having a bent portion formed therein and having a confluence formed therein, the upper portion of which is connected to an intersection region of the main channel; The separation channel portion is formed separated in communication with the cross-area of the main channel is added to the bottom of the second bending direction angled to the main channel formed; is configured to include a.

The first bent portion and the second bent portion may be formed with curved surfaces.

In addition, the main channel width is formed to be 3 ~ 4mm, the delay channel is formed in the length between the first bent portion and the joining portion 3 ~ 4mm, the separation channel is between the second bent portion and the separation portion The length of can be formed into l ~ 2mm.

The delay channel may include a length of the delayed channel width between the first bent portion and the joining portion.

The separation channel may be formed to be 1-1.5 times long, and the separation channel may be spaced apart from the main channel at intervals such that the length between the second bend and the separation part is 0.3 to 0.5 times the main channel width.

In addition, the intersecting area of the main channel may be formed from a point where the separating part is positioned above the confluence part, and the separating part forms a confluence part or intersects with an extended part of the extended channel.

In addition, the portion between the confluence portion in the first bent portion of the delay channel and the portion between the separation portion and the second bent portion of the separation channel may be formed in a direction perpendicular to the main channel. In addition, the splitter of the splitter channel may have an extended part formed at a lower end of the splitter channel extending from the point opposite to the point where the joining part starts to the second bent part.

The delay channel may be formed to have an upward slope in the direction of the joining portion at the first bent portion, and the separation channel may be formed to have an upward slope in the direction of the second bend portion at the separation portion.

In addition, an additional protrusion protruding in the center direction of the main channel may be further formed on the upper wall of the delay channel communicating with the main channel so that the fluid that joins the delay channel is further introduced into the main channel center direction and flows.

In addition, the main channel has a length of 2 to 5 mm from the intersection to the bottom thereof.

The sample dosing area can be removed and the sample dosing area replaced by a partial overlap of the bond pads at the bottom of the main channel.

In addition, the main channel is adjacent to the intersection area between the intersection area and the diagnosis area.

Form a separation area to separate the channel in the portion, and by the bonding pad

The top and bottom of the separation zone can be connected.

Effects of the Invention

[24] The pattern structure of the diagnostic sensor of the present invention by the above solving means,

[25] By changing the pattern structure, the sample and reaction material have a time difference,

By transmitting the target material, the process of fixing the target material and the immobilized target material react with each other so that the detection process of luminescence or coloration is performed separately.

[26] This can improve the detection sensitivity by passing the sample and reaction material to the detection area at the same time and improving the end point of the existing pattern structure whose detection sensitivity is lowered due to the interference between the materials by supplying the time difference, and separating various substances sequentially. Supply

It is possible to provide a pattern structure in which accurate and precise diagnosis can be made by minimizing non-specific reactions caused by the mixed supply.

Brief description of the drawings

1 to 7b show a pattern structure according to a preferred embodiment of the present invention.

Floor plan.

8 is a plan view showing various pattern structures according to an embodiment of the present invention.

9 is a photograph showing a fluid flow process according to various patterns according to an embodiment of the present invention.

10 is a graph showing the results of measuring the flow rate and flow rate of various delay pads according to the embodiment of the present invention.

11 repeats the flow rate and flow rate of a selected delay pad according to an embodiment of the present invention.

Graph showing the results of the experiment.

12 is a photograph illustrating a fluid flow process based on a design and time difference using a fluorescent material according to an embodiment of the present invention.

Best Mode for Carrying Out the Invention [33] The present invention will be described in more detail with reference to the accompanying drawings. However, the accompanying drawings are only illustrative of the technical spirit and scope of the present invention, and the technical scope of the present invention is limited thereto. It will be appreciated by those skilled in the art that various modifications and variations are possible within the scope of the technical idea of the present invention based on these examples.

[34]

1 is a plan view showing a pattern structure by printing according to a preferred embodiment of the present invention.

[36] As can be seen, the pattern structure according to the present invention (10) is based on paper printing technology.

It is formed by coating an organic solvent, and can be produced by various methods such as screen printing. In this case, various kinds of fluid flow can be used, and NC membrane (Nitrocellulose membrane;

'MILLIPORE' product) -Based (75, 95, 120, 180, 240) s to select and use.

[37] The fabricated pattern structure 10 includes a sample injection zone 21 and a diagnosis zone 22 formed therein.

And a main channel 20, a delay channel 30 for delaying the fluid for a certain time and then joining the main channel, and a separation channel 40 for separating the fluid of the main channel.

The main channel 20 is formed to be long in the vertical direction, and at one side thereof.

The sample injection zone 21 is formed and the opposite side: a diagnostic zone 22 is formed at the end to allow fluid to flow from the sample injection zone to the diagnostic zone. In FIG. 1, a search target is provided at the lower end of the main channel. To insert a sample containing

A sample injection zone 21 is formed, and a diagnostic zone 22 in which the detection material is coated on the opposite upper end is formed so that the fluid flows from the lower part to the upper direction. An intersecting region 23 is formed in the middle portion of the section in which the sample introduced into the region 21 flows upward to the capillary phenomenon so as to communicate with another channel. In the intersecting region 23, fluid is introduced from another channel or the main The fluid in the channel is separated and drained.

The delay channel 30 is arranged side by side on the lower side of the main channel, and the lower buffer input area 32 into which the buffer is inserted is the main movement area of the delay channel.

It is configured separately from the delay flow region 31. The delay channel 30

The buffer input area 32 and the delay flow area 31 use separate delay pads 50:

By connecting: Buffer delay pad 50 inserted into buffer input area ί 32

Passage increases the flow time and finally joins the main channel 20 with a time difference.

[0040] The delay channel 30 includes a first bending portion 33 whose upper portion is bent in the main channel direction.

The upper end of the delay channel is in communication with the intersecting area 23 of the main channel. The communicating portion of the delay channel and the main channel is called a confluence part 34.

[41] Next, the separation channel 40 is arranged side by side with the upper portion of the main channel, the lower portion is bent in the main channel direction by the second bent portion 41, and the lower portion is connected to the intersection region 23 of the main channel. The communicated portion is referred to as a separator 42.

The separation channel 40 is formed on one side of the main channel 20, but is located in a direction opposite to the direction in which the delay channel is formed. When the fluid is introduced into the main channel from the delay channel 30, the main channel ( 20), the flowing fluid is pushed to the separation channel 40 and separated to proceed.

[44]

As described above, the first bent portion 33 and the second bent portion 41 are formed in a curved surface to minimize the flow velocity in the process of switching the fluid flow direction as shown in FIG. 2. It is desirable.

[46]

[47]

In addition, the main channel 20, the delay channel 30 and the separation channel 40 is formed in a width of 3 to 5mm each formed in the upper and lower portions of the drawing, the first bent portion of the delay channel 30 Between the 33 and the confluence portion 34 is formed in a width of 1.5 ~ 2.5mm, and between the second bent portion 41 and the separating portion 42 of the separation channel 40 is formed in a width of 1.5 ~ 2.5mm.

[49] The width of the main channel, the delay channel, and the separation channel should be less than 3mm.

It is difficult to carry out two-stage reactions in a separated environment because the flow rate increases and the time from the introduction of the sample to the reaction is short.If the channel of each channel is formed to be 5 mm or more, the injected sample and solution are transferred to the diagnosis area along the channel. In order to require large capacity, it is preferable to form the above range.

In addition, the delay channel 30 is a fluid supplied through the buffer injection region (32)

Flowing upward, the first bending section 33 breaks the direction toward the main channel 20, and then joins the main channel, and the first bending section, which is a spaced interval between the vertically arranged main channel and the delay channel, The length between the convex part 34 and the convex part 34 is preferably 0.5 to 1 times the width of the main channel width. For example, when the main channel width is formed to be 4 mm, The length between the first and second bent portions 33 and 34 is less than or equal to 3 mm, and the fluid flowing in the delay channel 30 is joined. Failure to pass through the part 34 to a stable horizontal laminar flow can result in a mismatch between the fluid flowing into the delay channel and the fluid flowing into the main channel, and the length between the first bend part 33 and the confluence part 34 In case of forming more than 4mm, it is possible to flow into the main channel in stable laminar flow state, but the width of the channel structure itself is Since a disadvantage that the brushed, it joined to the first bent part of the length thereof vice is preferably formed over 3 ~ 4ram within the range.

[51]

[52] In addition, the delay channel 30 is less than the channel interval width between the lower portion of the U bend 33 and the delay channel 30 between the confluence portion 34 and the bend portion 33. The meaning of the fluid that is formed in a small width to join the main channel can further increase the force. For example, the width between the confluence 34 and the first bend 33 is 1.5 to 2.5 mm to form a fluid that merges into the main channel. To increase the flow rate of the

Increasing the force to push the flowing fluid to one side leads to an increase in the flow rate of the fluid flowing through the main channel and the flow rate of the fluid flowing through the confluence of the main channel. In the case of forming more than 2.5 mm, it is preferable to form the width in the above range because the flow velocity of the fluid flowing into the joining portion is low, and the force pushing the fluid flowing through the existing main channel is low.

The separation channel 40 is disposed between the vertically arranged main channel and the separation channel.

The distance between the second bend portion 41 and the separation portion 42, which is the separation interval,

For example, when the main channel width is about 4 mm (3 to 5 mm), the length between the second bent portion 41 and the separating portion 42 may be 1 to 2 mm. If the length between the second bent portion 41 and the separating portion 42 is less than lmm

As the sample fluid supplied to the main channel 20 is moved upward, the amount of discharged to the separation channel 40 increases, thereby reducing the amount of sample flow to the diagnostic region 22 located above the main channel. The fluid flowing through the main channel 20 is discharged from the confluence part 34 or pushed toward the separation part 42 by the fluid of the soft channel, and flows in a diagonal direction from the upward flow to the separation part.

In this case, when the length between the second bent portion 41 and the separation portion 42 is longer than 2 mm, the fluid flowing in the diagonal direction strikes the separation channel wall, and then the horizontal direction. this considerable resistance in the process of the flow of the to be done because the inflow into the separation unit is generated such resistance is also fluid entering portion separated _delivered: a significant amount by control fluid portion interfere with the entry of the main channel is not divided into separate channels Therefore, it is preferable that the length between the second bend portion 41 and the separation portion 42 is within the range of 1 mm to 2 mm because it affects the diagnosis flowed with the fluid joined in the delay channel.

In addition, the width between the separation section 42 and the second bent section 41 of the separation channel 40 is formed at a ratio of 0.5 to 1.5 times the width of the main channel, and 4 mm with the main channel 3 to 5 mm. Since the width between the second bent portion and the separating portion is 2 to 6 mm, the width between the second bent portion and the separating portion is preferably 2 mm or less. It is hard to flow into the separation part by being pushed by the fluid joined in the delay channel, and when formed over 6mm

Even if the fluid does not merge in the delay channel, the amount of fluid flowing into the main channel proceeds to the separate channel, thereby increasing the amount of sample containing the analyte.

It is preferable to form the above range as required. In addition, the confluence part 34 of the delay channel formed in the intersecting area 23 of the main channel 20 and the separation part 42 of the separation channel are located at positions spaced apart from each other.

That is, the fluid of the main channel 20 is introduced from the lower part to the upper part, and must be discharged by the fluid introduced from the confluence part 34 to be separated and discharged to the separating part 42. The confluence part 34 is formed in communication with the main channel 20, and the separation part 42 communicates with the upper part of the confluence part so that the fluid of the main channel located on the condensing part moves in a diagonal direction toward the separation part and discharges. This is done.

At this time, the forming position of the separating section 42 is formed so that the separating section is formed from the intersection point when the upper side of the main channel side wall consolidation section 34 is extended horizontally so that the vertical flow fluid and the joining section of the main channel are formed. When the horizontal fluid flows at and collides at the same flow rate, the vertical fluid flows in a diagonal direction so that it is separated and discharged to the separator.

[60]

Thus, the portion between the first bent portion 33 and the confluence portion 34 of the delay channel 30, and the portion between the separation portion 42 and the second bent portion 41 of the separation channel 40 are main Formed in a direction orthogonal to the channel 20, the fluid flowing horizontally at the joining portion can maximize the force that pushes the fluid moving up the main channel to the side.

As shown in FIG. 3, the expansion channel 43 is formed at the bottom of the separation channel 42 of the separation channel so that the moving fluid of the main channel driven by the fluid introduced from the confluence unit can be easily separated. The expansion portion 43 may be introduced into the channel.

Expansion can be made to the portion where the second bent portion 41 starts, so that expansion can be made from the position where the fluid push from the confluence portion starts.

[63]

In addition, as shown in FIG. 4, the upward inclination between the first bend portion 33 and the confluence portion 34 of the phase delay channel, and between the separation portion 42 and the second bend portion 41 of the separation channel. It can be formed to have.

That is, since the fluid flowing through the main channel 20 flows from the lower part to the upper part, the flow rate of the fluid flowing through the main channel decreases due to the fluid inflow from the delay channel through the confluence part 34. In order to minimize, it can be merged in a non-vertical inclination direction, and the upward inclination angle formed with the main channel is formed at an angle of 30 or more that can cause side sliding, and prevents a sudden drop in flow rate due to confluence. It can be formed below 60 so that

[66] By forming the confluence or slanting the channel portion in this way

The fluid discharged from the delay channel is less likely to push the main channel upwards to the side, so that separate discharge at the separation part may not be easily achieved. Therefore, as shown in FIG. 5, the delay channel 30 further includes a protrusion 35 in which the upper wall forming the confluence part 34 has a predetermined width in the main channel direction, and joins in the delay channel. By allowing the internal fluid to flow further into the main channel center direction, the existing fluid flowing into the main channel can be evenly pushed to the side. The protrusion 35 is projected at a ratio of 0.2 to 0.4 times the width of the main channel. When protruding below, the degree of penetration into the main channel center direction is low, forming a protruding portion. If the accuracy is low and projected more than 0.4 times, excessive projection may cause a problem that the fluid introduced into the main channel through the confluence may be mixed with the fluid vertically flowing from the bottom of the main channel to the projection. It is desirable.

[68]

As shown in FIG. 6, sample input to the main channel is separately determined.

The sample input area was removed by providing a contact pad (Conjugate pad: 60), and the length from the bottom of the main channel 20 to the conduit 34 of the delay channel 30 was formed shortly. (23) Short formation of the lower part shortens the time that the fluid introduced through the bonding pad 60, which overlaps the lower end of the main channel, enters the intersecting area 23, to the point where the fluid is joined from the delay channel 30. Can further increase the time interval.

For example, the widths arranged in the vertical direction in the main channel 20, the delay channel 30, and the separation channel 40 are 4 mm, and are formed between the low 11 bent portion 33 and the confluence portion 34. The delay channel width is 2 mm, and the separation part 42 is further formed with an expansion part 43 (a part connected from the inner channel inner wall surface facing the lower part of the joining part to the start point of the second bend part) having a 45 inclination. The width of the inlet is increased.

In addition, the main channel 20 is formed at 3 mm from the lower end to the start point of the confluence part 34, and the delay channel 30 is 3 mm from the beginning of the first bent part 33 to the lower part.

The buffer injection zone 32 was separated from the buffer injection zone 32, and the separation width was 1 mm. The buffer injection zone was formed to have a length of 5.5 mm in the upper and lower portions so as to sufficiently absorb the injected solution.

Wherein the adhesive pad (60; Conjugate pad) is attached to the lower end of the main channel

A delay pad 50 connecting the buffer input region 32 and the delayed flow region 31 of the delay channel;

Delayed release pads can be formed into squares with the same width and length as the main channel width. _:

In addition, when using an asymmetric membrane (Asymmetric Membrane, manufactured by PALL) with an adhesive pad and a delay pad, the length can be slightly increased to perform the filtering role of several sediment at the same time. If it is formed as 4mm, and the adhesive pad 60 and the delay pad 50 are used as asymmetric membranes, each of the adhesive pad and the retardation pad can be formed to have a width of 4 mm and a length of 4.5 mm. Here, the asymmetric membrane can be used. To filter silver plasma and sediment It is possible to select "Vivid GX, Viyid GR, Vivid GF" all of PALL products, and to adjust the flow rate of reaction and filtering of sediment, "Pore size of 0.45 / ffli, 0.8 ^ m, 8 / m asymmetric supermicron

Depending on the intended use, such as: "Asymmetric super micron polysulfone (MMM)" can be used.

In addition, the separation channel 40 is formed to be 21 mm from the point where the second bent portion 41 is formed to the top, so that the fluid pushed out by the fluid introduced from the delay channel 30 in the cross section 23 is introduced. It was made possible to proceed steadily.

In addition, the main channel may be positioned at the same horizontal level as that of the separation channel.

It can be extended to increase the flow distance of the fluid through the cross section.

[76]

As shown in FIG. 7, the main channel 20 may further form a separation region 24 between the intersection region 23 and the top.

The separation area 24 is positioned below the diagnosis area 22 through which detection is performed so that channels spaced up and down by an additionally placed adhesive pad 60 are connected to each other. The fluid passing through the intersecting area 23 can be flown to the diagnostic area 22 of the upper part by the adhesive pad 60 by being formed at a point of 4-5 mm from the separating part ₄ 2.

[79] As described above, the additional configuration of the delay pad 50 or the adhesive pad 60 in the channel flow section is performed by filtering and removing the precipitate contained in the sample or buffer solution to be added or the precipitate generated during the process. Detection sensitivity

To increase the flow rate of the fluid.

Therefore, the separation region is not limited to 1 or 7 in addition to the pattern 7 applied to the pattern of FIG. 6.

5 can also be applied to provide the above effects.

In addition, the size described in FIG. 7 is for one of the embodiments of the present invention, and the scope of the present invention includes not only the illustrated sizes but also changes of each size in the range of -20 to + 20%. something to do.

[82]

[83]

[84] Example

Experimental Example 1 Fluid Flow Measurement for a Pattern Structure

FIG. 8 is a diagram showing various pattern structures by printing an organic compound on an NC (nitrocelulos) membrane.

(A) The vertically arranged portions of the main channel, the delay channel, and the separation channel have a width of 4 mm, and are arranged to be spaced apart from each other by 4 mm. The space between the and separation sections was 2mm wide and formed to have a 45 degree upward slope from the first bend to the low 12 bend.

(B) forms a channel pattern in the same manner as in (A), but between the second bend and the separation part The separation channel width was formed to be the same 4mm width as the main channel.

In (C), a channel pattern 1 turn was formed in the same manner as in (A), but the distance between the vertically arranged separate channel and the main channel was set to 1.5 mm, and the outer angle of the second bent portion was formed as a curved surface. In addition, the confluence portion was formed with a protrusion projected to 1.5 mm of the main channel width in the center of the main channel.

[90] (D) The vertically arranged portions of the main channel, the delay channel and the separation channel have a width of 4 mm. The separation channel is spaced 1.5 mm apart from the main channel.

In addition, between the first bent portion and the confluence portion, and between the second bend portion and the separation portion, the width was 2 mm, and horizontally positioned so as to cross the main channel. The inflow to the separation channel was expanded.

Asymmetric super micron polysulfone with a pore size of 0.8 // m was used to connect the buffer-to-mip region and the delayed flow region of the delay channel.

[92]

[93] In order to understand the fluid flow, the main channel has an edible pigment above the sample injection zone.

blue and YM h) Surfactant (Product name: surfactant 10G from fitzgerald) was applied and dried. The delay channel had food coloring red and 1% (v / v) on the delay pad.

A surfactant (product name: surfactant 10G from fitzgerald) was applied and dried.

[94] I X in the sample input area of the main channel and the buffer input area of the delay channel, respectively.

Phosphate Buffered Saline (PBS) * was added to allow fluid to flow upward.

[95]

FIG. 9 illustrates the steep fluid flow process of the A, B, C, and D patterns of FIG.

It is a photograph.

[97] All four patterns first flowed in the main channel to the diagnosis area above the main channel, and before the time: after the delay, the fluid from the delay panel was supplied to the main channel to push the existing main panel fluid to one side. Fluid flow has occurred

In the case of the A pattern and the B pattern, the red fluid of the delay channel (fluid mixed with edible red) pushes the blue fluid of the main channel (fluid mixed with edible blue), so that a part of the blue fluid is weak. You can see it flowing to the same area.

[99] In the case of the C and D patterns, the red fluid introduced from the delay channel is

The blue fluid was pushed out to the side so that the blue fluid flowed only into the separation channel, and the D pattern had the best effect.

[100] In this way, all four patterns were routed from the delay channel to the main channel by the mimicked fluid.

It was found that the flowing fluid was pushed out to the separation channel by 70 to 100% and separated.

[101]

Experimental Example 2 Flow Measurement of Asymmetric Membrane

[103] Asymmetric super micron (Asymmetric super micron) selected from GALL, GX, GR and GF among Vivid as an asymmetric membrane used as a delay pad. The polysulfone was selected as the pore sizes 0.45, 0.8, and 8, respectively, and represented as GR, GX, GF, 0.45, 0.8, and 8, respectively.

[104] The D pattern of Experimental Example 1 is applied to each selected asymmetric membrane, but additionally

In the main channel, the lower part of the intersecting area has a length of 3 mm, and the shape of the shape is 6, and the lower part of the main channel has the adhesive pads partially overlapped, and the adhesive pad is the same as the soft pad.

[105]

[106] The adhesive pad at the bottom of the main channel is composed of lOug / ml STA-650 (Dylight fluorescent dye).

lOKDa Polyvinylpyrrolidone (PVP) and 0.5% (v / v) surfactant (product name: surfactant 10G from fitzgerald) with 1% (ν / ν) concentration was distilled into 1X PBS (phosphate buffered saline) buffer and converted into lOul volume. Dry at 37 C.

The delay pad of the delay channel is 10ug / ml STA-550 (fluorescent dye of Dylight), PVP (Polyvinylpyrrolidone) with lOKDa 1% (ν / ν) concentration, 0.5% (v / v) type ¾ active agent ： Surfactant 10G) from fitzgerald was diluted in 1 × PBS (phosphate buffered saline) buffer and dried at 37 C with a volume of lOul.

[108]

[109] The adhesive pad placed at the bottom of the main channel and the buffer input area of the delay channel are input by using 1 X PBS buffer, and the time when the fluorescent dye flows out of the delay pad at l ffiin interval (DP flow) and the delay channel. The rate at which the fluid flowing through the main channel is pushed to the separation channel by the fluid introduced in Fig. 10 is measured.

[10] As shown in FIG. 10, the asymmetric membrane of the delay pad is the latest. GF with fluorescent dye and 0.45 were selected, and the final selection of GF with less than 10 minutes in which the existing fluid flowing through the main channel was pushed as a separate channel was selected.

[111]

[112] Fluorescent dyes from delay pads were applied to selected GF asymmetric membranes.

The repeated outflow experiment (DP flow) and the time in which the fluid flowing through the main channel by the fluid introduced from the delay channel is pushed into the separate channel (Ratio) are shown in Fig. 11 several times.

[113] In the repeated experiments, all five measurements showed similar measurement values.

GF was selected as an asymmetric membrane to be used as a delay pad.

[114]

Experimental Example 3) Check the fluid flow of the pattern

Asymmetric membrane GF was used as the delay pad and the adhesive pad in the pattern structure of FIG. 6, and the rest was tested in the same conditions as Experimental Example 2, and the fluid flow was measured at 12-minute intervals.

As mentioned, the main channel is first driven by fluid in the adhesive pad. Flowed into the upper diagnostic area and passed through the delay pad from 2 minutes elapsed. As the fluid was introduced from the side of the main channel, the fluid that predominantly flowed the main channel began to be pushed to one side. The fluid passing through the adhesive pad pushed to one side was separated into the separation channel, and after 11 minutes had elapsed. In the upper diagnosis area of the main channel, only the fluid flowing from the delay channel was transferred, and the fluid passing through the adhesive pad was separated into the separation channel and flowed.

[118]

Therefore, it is necessary to supply different materials in the diagnosis area sequentially so that easy diagnosis can be made by applying a diagnosis method capable of accurate detection.

[120]

Claims

Claim

[Claim 1] In the pattern structure of a diagnostic sensor in which a channel is formed using printing technology on paper,

It is formed long in the vertical direction to flow the fluid from the lower part to the upper part, and the sample input area (21) for introducing the sample is formed at the lower end, the diagnostic region (22) is formed at the upper end is coated with the detection material, An intermediate portion (23) formed in the middle portion of the intersecting region (23) connected to another channel;

The delay channel 31 is formed side by side on the main channel, and is separated from the delay flow region 31, which is a main movement region, and the buffer injection region 32 into which the buffer is inserted, spaced apart from the lower end of the delay flow region. It connects the two regions so that the time for the buffer to be transferred to the main channel is delayed, and the upper portion has a first bent portion 33 which is bent in the main channel direction so that the upper portion has a confluence portion 34 which communicates with the cross region of the main channel. Delay channel 30; A second bent part 41 is formed side by side on both sides of the main channel opposite to the delay channel, and the lower part is formed with a second bent part 41 bent in the main channel direction, and the lower part is in communication with the cross section of the main channel. Pattern structure comprising a separate channel (40) having a.

[Claim 2] The method according to claim 1,

The first bent portion (33) and the second bent portion (41) is a pattern structure characterized in that the bent portion is formed in a curved surface.

[Claim 3] The method of claim 2,

The main channel width is 3 ~ 4mm,

The delay channel 30 has a length between the low U bend 33 and the confluence 34.

3 ~ 4mm

The separation channel 40 has a length between the second section 41 and the separation section 42.

Pattern structure characterized by forming from 1 to 2 mm.

4. The method of claim 3, wherein

The intersecting area 23 of the main channel places the separator 42 above the confluence 34, wherein the separator is formed from a point where it forms a confluence or intersects with the extended portion of the extended channel 30. Characterized by a pattern structure.

5. The method of claim 4,

A portion between the first bent portion 33 and the confluence portion 34 of the delay channel 30 and a portion between the separation portion 42 and the second bent portion 41 of the separation channel 40 are orthogonal to the main channel. Pattern structure characterized by being formed in a direction.

6. The method of claim 5,

The lower part of the separating part 42 of the separating channel 40 extends from the point at which the joining part 34 is started to the point opposite to the point at which the second bent part 41 is started. A pattern structure characterized in that the expansion portion (43) is formed.

7. The method of claim 4,

The delay channel 30 is formed to have an upward slope in the direction of the joining portion 34 in the first bent portion 33,

And the separating channel (40) is formed to have an upward slope in the direction of the second bent portion (41) in the separating portion (42).

8. The method of claim 4,

The upper wall of the delay channel 30 in communication with the main channel 20 is further formed with a projection 35 projecting in the main channel center direction to flow the fluid that joins in the delay channel in the main channel center direction to flow. Pattern structure.

[Claim 9] The method according to claim 4,

The main channel 20 has a length from the cross section to the bottom of 2 to 5 mm to remove the sample input region, and to place the bonding pad 60 at the bottom of the main channel to partially overlap the sample input region. Pattern structure.

[Claim 10] In paragraph 4,

The main channel 20 further forms a separation region 24 separating the channel between the intersection region 23 and the diagnosis region 22 in a portion adjacent to the intersection region, and is formed by the bonding pads 60 at the upper and lower portions of the separation region. The pattern structure, characterized by the fact that is connected.