WO2018045186A1 - Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing - Google Patents

Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing Download PDF

Info

Publication number
WO2018045186A1
WO2018045186A1 PCT/US2017/049641 US2017049641W WO2018045186A1 WO 2018045186 A1 WO2018045186 A1 WO 2018045186A1 US 2017049641 W US2017049641 W US 2017049641W WO 2018045186 A1 WO2018045186 A1 WO 2018045186A1
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
method
dimensional matrix
biological sample
stimulus
Prior art date
Application number
PCT/US2017/049641
Other languages
French (fr)
Inventor
George M. Church
Evan R. DAUGHARTHY
Richard C. TERRY
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201662381997P priority Critical
Priority to US62/381,997 priority
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2018045186A1 publication Critical patent/WO2018045186A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing c