WO2018042225A1 - Procédé d'identification de l'état d'œufs lors du processus d'incubation et système de mise en œuvre de ce dernier - Google Patents

Procédé d'identification de l'état d'œufs lors du processus d'incubation et système de mise en œuvre de ce dernier Download PDF

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Publication number
WO2018042225A1
WO2018042225A1 PCT/IB2016/055180 IB2016055180W WO2018042225A1 WO 2018042225 A1 WO2018042225 A1 WO 2018042225A1 IB 2016055180 W IB2016055180 W IB 2016055180W WO 2018042225 A1 WO2018042225 A1 WO 2018042225A1
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WO
WIPO (PCT)
Prior art keywords
eggs
incubation
egg
spectrum
reference markers
Prior art date
Application number
PCT/IB2016/055180
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English (en)
Inventor
Uladzimir RASTOUTSAU
Aliaksandr LUKYANAU
Sergey VINOGRADOV
Andrei Kosolobov
Nikolai GALITSKY
Roman Gromov
Original Assignee
Rastoutsau Uladzimir
Lukyanau Aliaksandr
Vinogradov Sergey
Andrei Kosolobov
Galitsky Nikolai
Roman Gromov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rastoutsau Uladzimir, Lukyanau Aliaksandr, Vinogradov Sergey, Andrei Kosolobov, Galitsky Nikolai, Roman Gromov filed Critical Rastoutsau Uladzimir
Priority to PCT/IB2016/055180 priority Critical patent/WO2018042225A1/fr
Publication of WO2018042225A1 publication Critical patent/WO2018042225A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
    • A01K45/007—Injecting or otherwise treating hatching eggs

Definitions

  • the invention relates to a greater extent to the sphere of poultry breeding and, in particular, to processes of egg selection for the incubation and egg incubation itself.
  • the poultry breeding industry faces a number of problems related to improvement of the production process efficiency, product quality and environmental friendliness of the production.
  • the incubation process is an essential and a determining production stage, in which case the market requirements need to be taken into account. For example, what the market demands most is a higher production of hens, i.e. females. Selection of males after hatching requires extermination of a substantial portion of chicks. Christian Schmidt, the FRG Minister of Agriculture, stated in his speech that up to 45 million chicks were exterminated annually just because they have a different gender.
  • the most credible methods include the DNA analysis in embryonic fluid, for example, by using nuclear magnetic resonance and spectral resonance methods, including IR Raman spectroscopy, fluorescent spectroscopy and other methods (WO 2014021715 A2 Gender, Viability and/or Developmental Staged Termination of Avian Embryos in Ovo,).
  • IR Raman spectroscopy IR Raman spectroscopy
  • fluorescent spectroscopy fluorescent spectroscopy
  • Most nondestructive methods are designed to obtain spectra of embryos by irradiating them by different types of radiation of which IR irradiation is most frequently used (US 2013/0044210 HYPERSPECTRAL IDENTIFICATION OF EGG FERTILITY AND GENDER) and to compare these spectra with earlier obtained reference spectra.
  • the same problems relate to the method of egg candling in order to obtain images of embryos and their internal structure and to determine their sexual character based on this.
  • the gender of the to-be-incubated chicks needs to be identified prior to setting the eggs in the incubator.
  • the objective of the invention is to accurately identify the egg's gender before setting and also to monitor the embryo development process during the entire incubation period.
  • Another objective of the invention is to assess productivity and developmental potency and also to obtain a healthy animal.
  • An additional objective of the invention is to control the incubation process and identify the eggs in which the embryo development is stopped or delays or specific developmental abnormalities, diseases, damages, etc. occur.
  • identification of the state of eggs includes passive recording of the dynamic electromagnetic spectrum on an egg surface, wavelet spectrum transform and spectral-dynamic analysis of correspondence of the transformed spectrum to reference markers corresponding to male or female gender of the future animal.
  • the dynamic spectrum is recorded periodically at the incubation stage, the spectrum is compared with reference markers of the corresponding development stages and then the eggs which are not suitable for further incubation are removed and discarded.
  • the essence of the claimed method resides in the use of the method of spectral-dynamic analysis of the spectra of an electromagnetic signal recorded on the egg surface using a passive electrode, i.e. without subjecting an egg to exposure of any character.
  • the method of spectral-dynamic diagnosis is known as one of the methods of medical diagnostics (the Ukraine Patent UA 34389 A, S.M. Zakirov, I.V. Orzhelsky, Bull. No. 1, 15.02.2001. The Method of Diagnosing and Correcting Wave Characteristics of the Study Objects and a Device for Implementing the Same).
  • the accuracy of this method may be improved according to the Eurasian Patent (EA 017369 THE METHOD OF IDENTIFYING AND TESTING SUB-PROCESSES IN A COMPLEX WAVE FIELD OF AN OBJECT, V.N. Rostovtsev, A.O. Lukyanov).
  • a method of forming reference markers for identifying the state of eggs which includes recording electromagnetic spectra of a control batch of eggs, processing thereof and isolating reference markers, characterized in that the dynamic electromagnetic spectra on the egg surface are passively recorded at different stages prior to and in the process of incubation, wavelet transform of spectra, spectral dynamic analysis thereof are conducted and reference markers of practically significant states for each stage are formed and the obtained markers are verified based on respective characters of the hatched chicks after completion of the incubation.
  • a sexual character embedded on chromosomes is also available among multiple markers.
  • this method is used to monitor a complete cycle of development from an egg to a chick with prominent sexual characters and isolate reference markers of the male and female gender identity and also specific stages of the embryo development, for example, formation of a nervous, a blood- vascular system and other systems.
  • individual spectra of eggs are recorded successively in the process of conveying eggs to the incubator.
  • the obtained results are used for selecting and sorting eggs based on characters of suitability for further incubation (to discard dead and defective eggs) and based on sexual characters.
  • a passive electrode successively contacts the surface of each egg or is disposed in immediate proximity to the egg surface within a specific period of time required for recording the signal.
  • An optimal time for recording a wave signal is from 3 to 30 seconds. In this case, a longer recording interval allows spectral data to be accumulated to statistically correct them and improve credibility of assessment of egg states or identify the gender.
  • the dynamic spectra of eggs are recorded simultaneously by multiple passive electrodes for multiple eggs orderly arranged, for example, in lines or matrixes. Then, the recorded spectra are converted and analyzed to compare them with reference markers. Further, eggs are sorted by discarding and removing substandard eggs.
  • Another advantage of this method is the controllability of the incubation process and timely sorting out the eggs in which abnormalities are detected.
  • the database of reference markers may also include markers of various infections, diseases and carriers of these infections and diseases. They may be timely recognized and eliminated.
  • An egg marking system may be additionally used for further passage of eggs along a technological chain.
  • Fig. 1 is a schematic view of the device for implementing the provided method for selecting eggs based on gender and developmental potency.
  • Fig. 2 is a system for implementing the provided method in the process of incubation.
  • the claimed device for recording spectral dynamic states of eggs 1 comprises an electrode 2, a signal processor unit 3 which includes a serially connected amplifier 4 and an analog digital converter 5 the output of which is connected to the input of a computer 6 with a processor and operation system 7 within the framework of which a comparator unit 8 is provided, and also an information-carrying medium 9 storing records of reference diagnostic markers.
  • the analysis result arrives to an indication or marking unit 10.
  • An actuating mechanism 11 for removing eggs may be also additionally integrated into the system.
  • a conveyer 12 is configured to transport eggs.
  • the provided device operates as described hereinafter.
  • the conveyer 12 moves and eggs pass by the electrode 2, the conveyer stops for a specific period of time and the electrode 2 contacts (directly or through a pad) the surface of an egg 1.
  • An electromagnetic wave signal of the audio range (20 Hz - 11 KHz) arrives to the input of the signal processor unit 3 and is fed to the broadband low-noise amplifier 4.
  • An amplified signal arrives to the input of the analog-digital converter 5.
  • a digitized signal via a USB port arrives to the computer 6 to the input of the comparator unit 8 which sends it to the processor 7 with an operation system configured to provide the spectrum wavelet transform and compares wavelet spectrograms of an input signal with a reference diagnostic marker previously selected from the information-carrying medium 9, thereby recognizing an image of the reference diagnostic marker in the spectral dynamic structure of an input signal from the egg 1.
  • the results of the spectral-dynamic analysis arrives to the indication or marking unit 10 which either illuminates the egg or marks it.
  • the actuating mechanism 11 either let the egg pass for further incubation process by actuating the conveyer 12 or excludes it from the technological process.
  • Fig. 2 a scheme shown in Fig. 2 may be used.
  • eggs 1 are arranged in cells on a carrier suitable for incubation.
  • Passive measuring electrodes 2 are simultaneously or successively moved to proximity of a batch of eggs 1, with these electrodes being connected to reversible amplifiers 12 and AD converter 13 (ADC-DAC) which are connected to switching and communication devices 14 which in their turn are coupled to a processing unit 15 configured to control, convert and compare dynamic spectra with reference markers from the database 16.
  • ADC-DAC AD converter 13
  • the system may operate in the automatic mode displaying the process on a terminal 17 or being controlled from this terminal.
  • Passive electrodes 2 may also be mounted in egg cells and switch themselves simultaneously or successively with amplifiers 12.
  • the system operating principle is as described hereinafter.
  • the eggs 1 are arranged in cells for incubation.
  • Individual passive electrodes 2 configured to pick up the surface audio-range electromagnetic signal accompanying the egg 1 wave field are disposed in immediate proximity to the egg surface.
  • the picked up signals are amplified by respective reversible amplifiers 12 and are converted by the reversible AD converter 13 into a digital signal.
  • the time of signal recording is in the range of 3-30 seconds.
  • Digital signals are recorded in the data switch and communication devices 14, are controlled and sent to the processing unit 15 configured to control, convert and compare dynamic spectra with reference markers from the database 16.
  • the processing unit 15 provides the wavelet transform of the spectrum of each egg and compares wavelet spectrograms of each input signal with a reference diagnostic marker or a group of markers previously selected from the information-carrying medium 9, thereby recognizing an image of the reference diagnostic markers in the spectral dynamic structure of an input signal from each egg 1.
  • a reference diagnostic marker or a group of markers previously selected from the information-carrying medium 9 thereby recognizing an image of the reference diagnostic markers in the spectral dynamic structure of an input signal from each egg 1.
  • Both markers of gender at different incubation stages and markers of different typical state of eggs of the given species and also markers of different egg diseases may be used as reference markers.
  • the degree of development of states and/or diseases is also assessed and eggs having developmental abnormalities or eggs in which development is either arrested or a specific disease manifested itself or there exists the risk of disease occurrence are identified.
  • the eggs having considerable abnormalities are removed from the incubator.
  • the treatment may be provided by exposing a selected egg to a compensatory or resonance reverse effect of the reversed signal formed from the reference marker by reversed wavelet transform thereof into a digital signal and further by converting it into an analog signal by the DA converter, by standardizing the amplitude with the reversible amplifier 12 and sending the resultant signal to the electrode 2 of the selected egg in antiphase when exposing the egg to compensatory effect and in-phase when exposing it to resonance effect.
  • This procedure is conducted until the abnormality is compensated or eliminated or it is stopped if no improvement is detected. In the latter case, the egg is removed from the incubator. Detected abnormalities are displayed on the terminal screen 17 which is also configured to send respective action instructions.
  • the degree of integration of the scanning and analytical parts of the system and also control procedures and resources distribution are defined by the scale of production, incubation period and embryo development stages, i.e. by stages of formation of different embryo system.
  • it is reasonable to make the analog-digital part comprising units 12, 13, 14 mobile and transmit digitized signals by wireless channels to the analytical complex. This allows for monitoring the incubation process and timely detect deviations from the embryo development process, possible diseases and damages. This improves process efficiency, provides actually 100% yield of healthy chicks, improves quality of products and helps protect the environment.
  • These control and correction systems also considerably help prevent the occurrence of mass diseases in chicks both during the incubation process and the following breeding.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Birds (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne des procédés de sélection d'œuf destiné à être incubé et l'incubation de l'œuf lui-même. L'objectif de l'invention est d'identifier avec précision le sexe de l'œuf avant la couvée et également de surveiller le processus de développement de l'embryon pendant toute la période d'incubation. L'identification de l'état des œufs comprend l'enregistrement passif du spectre électromagnétique dynamique sur une surface d'œuf, d'une transformée de spectre d'ondelettes et d'une analyse spectrale-dynamique de la correspondance du spectre transformé avec des marqueurs de référence correspondant au genre mâle ou femelle du futur animal. En outre, la productivité et les étapes de développement sont comparées à des marqueurs de référence. Il est tout à fait essentiel que le spectre dynamique soit enregistré avant incubation et comparé à des marqueurs de référence du genre et/ou de la productivité, puis que les œufs à incuber soient sélectionnés.
PCT/IB2016/055180 2016-09-03 2016-09-03 Procédé d'identification de l'état d'œufs lors du processus d'incubation et système de mise en œuvre de ce dernier WO2018042225A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2016/055180 WO2018042225A1 (fr) 2016-09-03 2016-09-03 Procédé d'identification de l'état d'œufs lors du processus d'incubation et système de mise en œuvre de ce dernier

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2016/055180 WO2018042225A1 (fr) 2016-09-03 2016-09-03 Procédé d'identification de l'état d'œufs lors du processus d'incubation et système de mise en œuvre de ce dernier

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WO2018042225A1 true WO2018042225A1 (fr) 2018-03-08

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110570027A (zh) * 2019-08-21 2019-12-13 安庆永强农业科技股份有限公司 基于数据分析的高产蛋率番鸭选育方法
WO2021214773A1 (fr) * 2020-04-24 2021-10-28 N.R Soos Technology Ltd. Incubateur d'éclosion d'œufs ayant un bruit de fonctionnement réduit

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6029080A (en) * 1997-07-09 2000-02-22 Reynnells; Richard D. Method and apparatus for avian pre-hatch sex determination
US7041439B2 (en) * 2001-04-17 2006-05-09 Embrex, Inc. Methods and apparatus for selectively processing eggs having identified characteristics
US20140069336A1 (en) * 2012-08-31 2014-03-13 Mat Malta Advanced Technologies Limited Spectrophotometric analysis of embryonic chick feather color

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6029080A (en) * 1997-07-09 2000-02-22 Reynnells; Richard D. Method and apparatus for avian pre-hatch sex determination
US7041439B2 (en) * 2001-04-17 2006-05-09 Embrex, Inc. Methods and apparatus for selectively processing eggs having identified characteristics
US20140080165A1 (en) * 2001-04-17 2014-03-20 Embrex, Inc. Methods and apparatus for selectively processing eggs having identified characteristics
US20140069336A1 (en) * 2012-08-31 2014-03-13 Mat Malta Advanced Technologies Limited Spectrophotometric analysis of embryonic chick feather color

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110570027A (zh) * 2019-08-21 2019-12-13 安庆永强农业科技股份有限公司 基于数据分析的高产蛋率番鸭选育方法
CN110570027B (zh) * 2019-08-21 2022-10-25 安庆永强农业科技股份有限公司 基于数据分析的高产蛋率番鸭选育方法
WO2021214773A1 (fr) * 2020-04-24 2021-10-28 N.R Soos Technology Ltd. Incubateur d'éclosion d'œufs ayant un bruit de fonctionnement réduit

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