WO2018030683A1

WO2018030683A1 - Pharmaceutical composition comprising thymidine phosphorylase (tymp) protein as effective ingredient for prevention or treatment of hair loss

Info

Publication number: WO2018030683A1
Application number: PCT/KR2017/008117
Authority: WO
Inventors: 송순욱; 성종혁
Original assignee: 에스씨엠생명과학 주식회사
Priority date: 2016-08-08
Filing date: 2017-07-27
Publication date: 2018-02-15
Also published as: KR101671361B1

Abstract

The present invention relates to a composition comprising a TYMP protein as an effective ingredient for prevention, treatment, or alleviation of hair loss and for promotion of hair growth and hair development. A composition comprising a TYMP protein as an effective ingredient for prevention of hair loss and promotion of hair growth according to the present invention shows an outstanding hair growth effect within a short time without side effects upon use in the treatment of hair loss and thus can be effectively used as a drug as well as in quasi-drugs, cosmetics, and health functional foods.

Description

[Revision according to Rule 26.08.2017] A pharmaceutical composition for preventing or treating hair loss comprising TYMP (Thymidine Phosphorylase) protein as an active ingredient

The present invention is a pharmaceutical composition for preventing or treating hair loss, a composition for preventing or improving hair loss, a composition for preventing or improving hair loss, a composition for promoting hair growth or promoting hair loss, a method for preventing or treating hair loss, a method for improving hair loss, and a hair growth comprising a thymidine phosphorylase (TYMP) protein as an active ingredient It is about hair growth promotion method.

Hair loss refers to a condition in which hair is not normally present in the area where hair is normally present, and generally means that the hair of the scalp (coarse black hair) falls out. Unlike virgin hair, which has no color and thick color, it can cause cosmetic problems if it falls out. Koreans with lower hair density than Westerners have 5-70,000 hairs, and about 50-70 hairs per day are normal. Therefore, hair loss may be in progress if the number of hairs that fall after sleeping or when you wash your hair exceeds 100.

There are many causes of hair loss, and genetic causes and androgens (androgens) are considered to be important factors for baldness, and some of the female hair loss is believed to occur in the same path as male hair loss. There is a difference in aspects. Alopecia areata is thought to be an autoimmune disease. Resting alopecia is a temporary hair loss that occurs after severe physical and mental stress such as endocrine disease, nutritional deficiency, drug use, childbirth, fever, and surgery.

In addition, among the symptoms of the hair loss, male-type hair loss is gradually thinning hair from the 20s or 30s in people with a family history of baldness, hair loss proceeds. As the forehead and head line is pushed backward, the forehead widens in the M-shape with both temporal heads and hair loss progresses gradually in the crown of the head. Female hair loss is characterized in that the hairline on the forehead is thinned and hair thinner compared to male hair loss. In addition, female hair loss is so weak that hair loss is rare and complete baldness, as in male hair loss. Alopecia areata is also characterized by the development of round or oval bald spots of varying sizes (hair loss that looks like dots). It usually occurs on the head, and rarely can occur on the beard, eyebrows or eyelashes, and the enlarged area of symptoms may cause large bald area to form. If the entire hair falls out, alopecia areata (frontal alopecia), if the hair falls out of the systemic hair loss. In addition, alopecia areata starts hair loss from 2 to 4 months after causative stimulus occurs, and hair thinning is reduced overall. As the causative stimulus is removed, hair loss is reduced as restorative hair returns to normal for several months.

On the other hand, as a conventional treatment for hair loss, a male or female hair loss drug such as minoxidil, a drug such as finasteride, a hair transplant, and the like are used. For the treatment of alopecia areata, topical steroid preparations, systemic steroid preparations, immunotherapy and the like are used. Resting alopecia causes hair to recover when the cause is removed, so it is important to identify and treat the cause. However, when treating hair loss by such a conventional method, there is a problem of side effects because it depends on drug therapy, steroid preparations, etc. In addition, there is a problem that the treatment cost also increases significantly if the treatment using drugs or hair transplantation. Minoxidil and finasteride are representative drugs used to promote hair growth. Minoxidil from the US Upzone may cause side effects such as swelling, arrhythmia and unwanted hairs on long term application.The effect of minoxidil is most effective between 6 months and 1 year from use and then gradually It is known that the effect is reduced. Finasteride, developed by Merck, is known to inhibit the activity of 5-α-reductase, an enzyme that acts on male hormone testosterone metabolism in hair follicles. An increase in suicidal thoughts has been reported. In addition, it is not applicable to women of childbearing age or pregnant women because it increases the probability of birth of birth defects. Moreover, it is known that hair loss progresses again if both drugs are discontinued. There is also Valproic acid, but taking it during pregnancy is known to greatly reduce the child's cognitive development ability. As mentioned above, various types of conventional hair regrowth have been attempted to help hair growth for the purpose of promoting blood circulation of the scalp and nourishing hair roots. However, the present invention is also toxic and side effects are severe and the effect is insufficient. .

Accordingly, the present inventors have made intensive efforts to develop a material capable of preventing hair loss and promoting hair growth without side effects, and confirmed that the TYMP (thymidine phosphorylase) protein present in the body has an effect of promoting hair growth. Completed.

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating hair loss comprising TYMP (thymidine phosphorylase) protein as an active ingredient.

Another object of the present invention is to provide a quasi-drug composition, cosmetic composition and food composition for preventing or improving hair loss containing TYMP protein as an active ingredient.

Another object of the present invention to provide a pharmaceutical composition, quasi-drug composition, cosmetic composition and food composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

Still another object of the present invention is to provide a method for preventing or treating hair loss, a method for improving hair loss, and a method for promoting hair growth or hair growth, comprising: treating a subject with TYMP protein.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the prevention or treatment of hair loss comprising TYMP (thymidine phosphorylase) protein as an active ingredient.

In addition, the present invention provides a quasi-drug composition for preventing or improving hair loss comprising TYMP protein as an active ingredient.

The present invention also provides a cosmetic composition for preventing or improving hair loss comprising TYMP protein as an active ingredient.

In addition, the present invention provides a food composition for preventing or improving hair loss comprising TYMP protein as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

In addition, the present invention provides a quasi-drug composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

The present invention also provides a cosmetic composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

In addition, the present invention provides a food composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

The present invention also provides a method for preventing or treating hair loss, comprising the step of treating the subject with TYMP protein.

In addition, the present invention provides a method for improving hair loss comprising the step of treating the subject with a TYMP protein.

In addition, the present invention provides a method for promoting hair growth or hair growth comprising a; treating the subject with a TYMP protein.

The composition for preventing hair loss or promoting hair growth comprising TYMP (thymidine phosphorylase) protein according to the present invention as an active ingredient has no side effects when treating hair loss, and can be used as a medicine due to its excellent hair growth effect even in a short time. It can be usefully used for health functional food.

Figure 1 is a diagram showing the results of measuring the cell proliferation for 40 hours after treatment with TYMP protein DPC.

Figure 2 is a diagram showing the dorsal portion of the mouse after 15 days after subcutaneous injection of TYMP protein after removing hair using a hair removal agent in the back of C3H / HeN mice.

Figure 3 is a diagram showing the result of measuring the increased hair growth weight (mg) of the mouse after 15 days after subcutaneous injection of TYMP protein after hair removal using a hair removal agent in the back of C3H / HeN mice.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing or treating hair loss and promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

TYMP (thymidine phosphorylase) is an enzyme that reversibly converts thymidine into thymine and 2-deoxyribose-1-phosphate and is known to be involved in DNA synthesis. In addition, high expression of TYMP was observed in various solid tumors, centering on gastric cancer and colorectal cancer, and positive expression correlated with angiogenesis, invasion, metastasis, and expression of TYMP was one of the prognostic factors. Was found to be. In relation to other conditions, high expression at the site of the disease has been reported, mainly in inflammatory diseases, in patients with glomerulonephritis, interstitial cystitis, chronic arthritis rheumatism, and inflammatory bowel disease. However, it has not been reported that TYMP is associated with hair loss symptoms and hair growth effects.

The TYMP protein may consist of all or part of a peptide having a prophylactic effect of preventing or treating hair loss, hair growth or hair growth, and may preferably be composed of the amino acid sequence of SEQ ID NO: 1, and functional equivalents and variants of the protein. It includes.

Functional equivalents of the proteins include proteins and polypeptides that do not alter the activity of the protein molecule as a whole, and proteins that can function functionally are included in the scope of the present invention. The variant may be, for example, one or several amino acids deleted, substituted or added to the amino acid sequence, having 95% or more, preferably 98% or more, and more preferably 99% or more identity with the amino acid sequence. Say things. Here, "identity" means that the other amino acid sequence includes the gap between two amino acid sequences or the other amino acid sequences, including the number of gaps, when the gap is introduced to the highest amino acid sequence. The ratio (%) of the number of the same amino acid residue of the amino acid sequence of is referred to. In addition, "a few" means the integer of 2-10, for example, the integer of 2-7, 2-5, 2-4, 2-3. As a specific example of a natural variant, the variant based on polymorphisms, such as SNP (monobasic polymorphism), a splice variant, etc. are mentioned. Preferably, the substitution is a conservative amino acid substitution. It is because conservative amino acid substitutions can have a structure or property substantially equivalent to human TYMP which has the said amino acid sequence. Conservative amino acids are non-polar amino acids (glycine, alanine, phenylalanine, valine, leucine, isoleucine, methionine, proline, tryptophan) and polar amino acids (amino acids other than nonpolar amino acids), charged amino acids (acidic amino acids (aspartic acid, glutamic acid) and basic) Amino acids (arginine, histidine, lysine)) and uncharged amino acids (amino acids other than charged amino acids), aromatic amino acids (phenylalanine, tryptophan, tyrosine), branched amino acids (leucine, isoleucine, valine) and aliphatic amino acids (glycine, alanine, leucine) , Isoleucine, valine), and the like are known. In addition, the protein may include a protein having increased structural stability or increased protein activity against heat, pH, etc. of the protein by variation or modification on the amino acid sequence.

The gene encoding the TYMP protein may be composed of the nucleotide sequence of SEQ ID NO: 2, variants which can function functionally the same as the nucleotides are included in the scope of the present invention.

The variant capable of the functionally identical function is at least 70%, preferably 80% or more, more preferably 90% or more of the nucleotide sequence represented by SEQ ID NO: 2 as a result of the addition, substitution or deletion of base. And, more preferably, having a sequence homology of 95% or more, it means a base sequence capable of encoding a protein that exhibits substantially homogeneous physiological activity with a protein encoded by the nucleotide sequence represented by SEQ ID NO: 2. For example, although some sequences have been modified by deletion, substitution or insertion, they include variants that can function functionally identical to nucleic acid molecules encoding the TYMP protein. It is a concept.

As used herein, the term 'hair loss' may fall under the normal hair growth level if the number of hairs is less than the general number without any particular limitation, and preferably consists of male hair loss, female hair loss, circular hair loss and resting hair loss. Any one or more selected from the group may correspond to hair loss being treated or prevented by the composition according to the present invention. In addition, the composition according to the present invention is also excellent in preventing hair loss before hair loss begins.

As used herein, the term 'hair growth' refers to hairy, and 'hair growth' refers to hair growth. The effect of the composition according to the invention also includes the effect of promoting hair growth or hair growth.

As used herein, the term "prevention" means any action that inhibits or delays hair loss by administration of a composition of the present invention, and "treatment" means that the symptoms caused by hair loss are improved or beneficially altered by the composition of the present invention. It means all actions.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. In addition, the pharmaceutical compositions according to the invention are each formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories, and sterile injectable solutions according to a conventional method. Can be used. Suitable formulations known in the art are preferably those described in the literature.

Carriers, excipients and diluents that may be included in the pharmaceutical compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulating the pharmaceutical composition according to the present invention, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. which are commonly used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose, lactose, gelatin, and the like in the active ingredient. Mix is prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solution solutions, emulsions, and syrups, and various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin, may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, etc. may be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition of the present invention may be administered in a pharmaceutically effective amount.

As used herein, the term “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dose level is determined by the type and severity, age, sex, disease of the individual. It can be determined according to the type, activity of the drug, sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including the drug used concurrently, and other factors well known in the medical field. The compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. And single or multiple administrations. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect in a minimum amount without side effects, and can be easily determined by those skilled in the art. The preferred dosage of the composition of the present invention depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration of time, and the suitable total daily dosage can be determined by the practitioner within the correct medical judgment. Generally, an amount of 0.001 to 1000 mg / kg, preferably 0.05 to 200 mg / kg, more preferably 0.1 to 100 mg / kg, may be administered once to several times daily. The object is not particularly limited as long as it is an object for the purpose of preventing or treating hair loss, and any object can be applied. For example, it can be applied to any individual, such as monkeys, dogs, cats, rabbits, marmots, rats, mice, cows, sheep, pigs, goats, and the like, and to humans, and the mode of administration is conventional in the art. If not included. For example, transdermal administration through topical application may be used, but is not limited thereto.

On the other hand, the method of administering the pharmaceutical composition according to the present invention is not particularly limited, and may be administered orally, or applied to the hair loss site or applied to the site where the progress of hair loss is foreseen. In addition, there is no particular limitation on the method of administration, and it is also possible to inject directly in the form of injection to the site where hair loss or progression of hair loss is foreseen. In addition, the method of administration is not particularly limited, so it is possible to inject by injection into an artery or vein.

The dosage level selected from the composition will also depend on the activity of the active ingredient, the route of administration, the severity of the hair loss being treated and the condition and previous history of hair loss being treated. However, it is within the knowledge of the art to start with a lower dose of active ingredient than is required to achieve the desired therapeutic effect, and gradually increase the dosage until the desired effect is achieved. It can be determined according to gender, body type and weight. The composition may be further processed before being formulated into a pharmaceutically acceptable pharmaceutical formulation, and may preferably be ground or ground into smaller particles. The composition will also vary depending on the condition and the patient being treated, but this can be determined non-originally.

The compositions of the present invention have formulations that can be administered by methods such as direct application or spreading on the skin, such as hair or scalp, such as creams, lotions, ointments, aerosols, shampoos, gels, or packs. It may be. Methods for the formulation or formulation suitable for each formulation are well known in the art. Those skilled in the art can appropriately select and use various combinations of ingredients used to prepare conventional external preparations in preparing these formulations.

Hair to which the composition of the present invention is applied includes hair follicles and hair follicles of the head, hair and eyelashes, and eyelashes, beards, armpits, pubic hair, and all parts of the hair root and hair follicles throughout the body.

The present invention also provides a quasi-drug composition for preventing or improving hair loss and promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

As used herein, the term "quasi drug" refers to a fiber, rubber product or the like used for the purpose of treating, alleviating, treating or preventing a disease of a human or animal, has a weak action on the human body or does not directly act on the human body, Or non-machinery and the like, or any of the agents used for sterilization, insecticide, and similar purposes for the prevention of infection, for the purpose of diagnosing, treating, reducing, treating or preventing human or animal diseases. Among articles to be used, which are not instruments, machines or devices, and articles which are used for the purpose of pharmacologically affecting the structure and function of humans or animals, except those which are not instruments, machines or devices. Also includes supplies.

When the composition of the present invention is included in a quasi-drug for the purpose of preventing or improving hair loss, promoting hair growth or hair growth, the composition may be used as it is or may be used together with other quasi-drug components, and may be appropriately used according to a conventional method. have. The mixing amount of the active ingredient can be appropriately determined depending on the intended use.

The quasi-drug of the present invention is not particularly limited thereto, but may be prepared and used, for example, in the form of a cream, lotion, aerosol, shampoo, gel or pack.

In the case of creams, ointments, shampoos, gels or packs, white petrolatum, yellow petrolatum, lanolin, bleached beeswax, cetanol, stearyl alcohol, stearic acid, hardened oil, gelled hydrocarbons, polyethylene glycol, liquid paraffin, squalane, etc. Mechanism of; Solvents and dissolving aids such as oleic acid, isopropyl myristate, glycerin triisooctanoate, crotamiton, diethyl sebacate, diisopropyl adipropyl, hexyl laurate, fatty acids, fatty acid esters, aliphatic alcohols and vegetable oils; Antioxidants such as tocopherol derivatives, L-ascorbic acid, dibutylhydroxytoluene and butylhydroxyanisole; Preservatives such as parahydroxybenzoic acid ester; Moisturizing agents such as glycerin, propylene glycol and sodium hyaluronate; Surfactants such as polyoxyethylene derivative, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester and lecithin; Thickeners such as carboxyvinyl polymer, xanthan gum, carboxymethyl cellulose, carboxymethyl cellulose sodium salts, hydroxypropyl cellulose, hydroxypropyl methyl cellulose and the like.

In the case of aerosols, white petrolatum, yellow petrolatum, lanolin, bleached beeswax, cetanol, stearyl alcohol, stearic acid, used in the preparation of ointments, creams, gels, suspensions, emulsions, solutions and lotions Bases such as hardened oil, gelled hydrocarbon, polyethylene glycol, liquid paraffin, squalane and the like; Such as oleic acid, isopropyl myristate, diisopropyl adipic acid, isopropyl sebacate, glycerin triisooctanoate, crotamiton, diethyl sebacate, hexyl laurate, fatty acids, fatty acid esters, aliphatic alcohols, vegetable oils Solvents and dissolution aids; Antioxidants such as tocopherol derivatives, L-ascorbic acid, dibutylhydroxytoluene and butylhydroxyanisole; Preservatives such as parahydroxybenzoic acid ester; Moisturizing agents such as glycerin, propylene glycol and sodium hyaluronate; Surfactants such as polyoxyethylene derivatives, glycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters, lecithin; Thickeners such as carboxyvinyl polymer, xanthan gum, carboxymethyl cellulose, carboxymethyl cellulose sodium salts, hydroxypropyl cellulose, hydroxypropyl methyl cellulose; In addition, various stabilizers, buffers, mating agents, suspending agents, emulsifiers, fragrances, preservatives, dissolution aids, and other suitable additives may be blended.

Moreover, a stabilizer, a preservative, an absorption promoter, a pH adjuster, and other suitable additives can be mix | blended as needed.

In addition, the present invention provides a cosmetic composition for preventing or improving hair loss and promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

The cosmetic composition is a skin external preparation formulation of hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder gel, hair pack, hair treatment, eyebrow regrowth, eyelash regrowth, eyelash It may be a formulation of a nutrient, but is not limited thereto.

In addition, the composition for preventing or improving hair loss, hair growth or hair growth promoting composition of the present invention can be used for pets different from the formulation. For example, it can be prepared in various forms such as solution, sol-gel, emulsion, oil, wax, aerosol, etc. such as pet shampoo and pet rinse. It can be prepared by addition.

In addition, the cosmetic composition of the present invention may include without limitation the conventionally acceptable ingredients in addition to the active ingredient, and includes conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. can do.

In the case of a shampoo, any one or more of a synthetic surfactant, a preservative, a thickening agent, a viscosity adjusting agent, a pH adjusting agent, a perfume, a dye, a hair conditioner, and water, which are cleaning components, may be contained. Among the synthetic surfactants, synthetic anionic surfactants include alkyl and alkyl ether sulfates, and representative examples thereof include sodium lauryl sulfate, lauryl ammonium sulfate, lauryl sulfate triethanolamine, polyoxyethylene lauryl sulfate, and polyoxyethylene lauryl ammonium sulfate. have. Among the synthetic surfactants, synthetic amphoteric surfactants are alkyl betamin and alkyl amidopropyl betaine, and representative examples thereof are cocodimethyl carboxymethyl betaine, lauryldimethyl carboxymethyl betaine, lauryl dimethyl alpha-carboxyethyl betaine. , Cetyl dimethyl carboxymethyl betaine, cocamidopropyl betaine and the like. The nonionic surfactants include alkanol amides and amine oxides, such as lauryl diethyl amine oxide, palm oil alkyldimethyl amine oxide, lauric acid diethanol amide, palm oil fatty acid diethanolamide, palm oil fatty acid monoethanolamide, and the like.

The optional ingredients used in the present invention are well known to those skilled in the art as conventional optional ingredients for maintaining basic physical properties and quality as shampoos. Such optional ingredients include pearlescent aids, preservatives, thickeners and viscosity adjusting agents, pH adjusting agents, perfumes, dyes, hair conditioning agents. Pearlescent aids such as, for example, ethylene glycol monostearate, ethylene glycol distearate; Preservatives such as methyl paraoxycyanate, methylchloro isothiazolinone and a mixture of methyl isothiazolinone; Thickeners and viscosity modifiers such as sodium chloride, ammonium chloride, propylene glycol; PH adjusters such as citric acid, phosphoric acid, sodium hydroxide, potassium hydroxide; Dyestuffs such as polyquaternium-10, polyquaternium-7, methylpolysiloxanes, dimethiconecopolyols, hair conditioning agents such as hydrolyzed animal proteins, water soluble tars are used, and fragrance may also be used.

The present invention also provides a food composition for preventing or improving hair loss and promoting hair growth or hair growth comprising TYMP protein as an active ingredient.

The food composition of the present invention may be in the form of a dietary supplement, and the "health supplement" refers to a food prepared and processed using raw materials or ingredients having useful functions to the human body according to Act No. 6767 of the Act on Dietary Supplement. "Functional" means ingestion for the purpose of obtaining useful effects on health use such as nutrient control or physiological action on the structure and function of the human body.

The food composition may further include food additives, and the suitability as a "food additive", unless otherwise specified, according to the General Regulations of the Food Additives Code and General Test Methods, etc. Judging by the criteria.

Items listed in the "Food Additives Code" include, for example, chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, cinnamon acid, natural additives such as navy, licorice extract, crystalline cellulose, guar gum, L- Mixed preparations, such as a glutamate sodium preparation, a noodles addition alkali preparation, a preservative preparation, and a tar pigment preparation, are mentioned.

Foods containing the active ingredient of the present invention includes breads, rice cakes, dried fruits, candy, chocolates, chewing gum, confectionery such as ice cream, ice cream products such as ice cream, ice cream powder milk, low fat milk, lactose milk, Processed Milk, Goat Milk, Fermented Milk, Butter Oil, Concentrated Milk, Milk Cream, Butter Oil, Natural Cheese, Processed Cheese, Powdered Milk, Dairy Products such as Whey, Meat Products, Processed Products, Meat Products such as Hamburgers, Ham, Fish products such as sausages, bacon and other processed meat products Ramen noodles, dried noodles, raw noodles, noodle soups, delicacies, dried noodles, improved noodles, frozen noodles, pasta noodles, fruit drinks, vegetable drinks, carbonated drinks, soy milk products, Lactic acid bacteria beverages such as yogurt, beverages such as mixed drinks, soy sauce, miso, red pepper paste, chunjang, cheongukjang, mixed soy sauce, vinegar, sauces, seasoned foods such as tomato ketchup, curry, dressing, margarine, But are the teuning and pizza, without being limited thereto.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And carbonating agents used in the carbonated beverage. In addition, the composition of the present invention may include a pulp for the production of natural fruit juice, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The beverage composition including the active ingredient of the present invention is not particularly limited to other ingredients other than the above-mentioned protein, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the aforementioned natural carbohydrates include monosaccharides (eg, glucose, fructose, and the like); Disaccharides (eg maltose, sucrose and the like); And conventional sugars such as polysaccharides (eg dextrin, cyclodextrin, etc.), and sugar alcohols such as xylitol, sorbitol, erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. have.

The present invention will be described in more detail with reference to Examples and Experimental Examples. However, the following Examples and Experimental Examples are provided to illustrate the present invention more easily, and the content of the present invention is not limited by the Examples. In this case, if there is no other definition in the technical and scientific terms used, The present invention has a meaning generally understood by those skilled in the art.

실시예 1.Example 1. TYMP의 수득Obtaining TYMP

TYMPine (thymidine phosphorylase) protein was purchased from Peprotech. The results of analyzing and confirming the amino acid sequence and the DNA base sequence of the obtained TYMP protein are shown in SEQ ID NOs: 1 and 2.

실시예 2.Example 2. TYMP의 세포증식 효과 확인Confirmation of Cell Proliferation Effect of TYMP

Human dermal papilla cell (DPC) proliferation experiments, a representative in vitro cell test system for measuring hair proliferation, were performed. 1 ng / ml or 5 ng / ml of the TYMP protein of Example 1 was treated with DPC, and the result of measuring proliferation for 40 hours is shown in FIG. 1.

As shown in FIG. 1, when TYMP protein was treated with DPC, it was confirmed that cell proliferation was increased compared with cells not treated with TYMP protein.

From the above results, it was confirmed that the TYMP protein has an excellent cell proliferation effect and thus can be used for hair loss treatment or promotion of hair growth.

실시예 3.Example 3. TYMP의 발모 효과 검증Validation of TYMP's hair growth effect

For experiments to confirm the hair growth effect, the hair of 7-week-old C3H / HeN mice was stabilized after hair removal using a depilatory agent. One day later, 100 ng / ml human thymidine phosphorylase (SEQ ID NO: 1) protein was injected subcutaneously once. As a control, phosphate buffered saline (PBS) containing 0.1% BSA in the same volume in which TYMP protein was dissolved was injected subcutaneously. Then, the degree of hair growth of the dorsal part of the depilated mice was observed for 15 days. The photograph of the back part of the mouse in which hair growth occurred is shown in FIG.

As shown in Figure 2, it was confirmed that the hair growth state is better in the group treated with TYMP protein than the control (control).

In addition, after removing hair using a knife on the back part of the newly growing hair after 15 days, the weight of the obtained hair was measured, and the results are shown in FIG. 3.

As shown in FIG. 3, the measured hair growth weight was found to be 90 mg in the TYMP protein treated group, compared to 40 mg in the control group, and it was confirmed that the hair growth promoting effect was excellent when the TYMP protein was treated. .

From the above results, it was confirmed that the TYMP protein component has an excellent hair growth effect and can be used as a composition for preventing and treating hair loss.

Hereinafter will be described a formulation example of a pharmaceutical composition for preventing or treating hair loss, a cosmetic composition for preventing and improving hair loss, and a food composition comprising the active ingredient of the present invention, but is not intended to limit the present invention, only to be described in detail. .

제제예 1. 약학적 제제의 제조Formulation Example 1 Preparation of a Pharmaceutical Formulation

1. Preparation of powder

TYMP protein 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

2. Preparation of Tablets

TYMP protein 10 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

3. Preparation of Capsule

TYMP protein 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

4. Preparation of Injectables

TYMP protein 10 mg

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Na ₂ HPO ₄ 2H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

5. Preparation of Liquid

TYMP protein 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding a proper amount of lemon aroma, and then mixing the above components, adding purified water and adjusting the whole to 100 ml by adding purified water and filling into a brown bottle. The solution is prepared by sterilization.

제제예 2. 화장품 제제의 제조Formulation Example 2 Preparation of Cosmetic Formulation

1. Hair Lotion Manufacturer

TYMP Protein 0.6 wt%

Glycerin 5.1 wt%

Propylene Glycol 4.2 wt%

Tocopheryl Acetate 3.0 wt%

4.6 wt% of liquid paraffin

Triethanolamine 1.0 wt%

Squalane 3.1 wt%

Macadamia Nut Oil 2.5% by weight

Polysorbate 60 1.6 wt%

Solbitan Sesquirrolate 1.6% by weight

Propylparaben 0.6 wt%

Carboxy vinyl polymer 1.5 wt%

A small amount of spices

Preservative traces

Purified water level

2. Hair Cream Manufacturing

TYMP Protein 1.0 wt%

Glycerin 4.0 wt%

Vaseline 3.5 wt%

Triethanolamine 2.1 wt%

53% by weight of liquid paraffin

Squalane 3.0 wt%

Beeswax 2.6 wt%

Tocopheryl Acetate 5.4 wt%

Polysorbate 60 3.2 wt%

Carboxy vinyl polymer 1.0 wt%

Sorbanthesquioleate 3.1 wt%

A small amount of spices

Preservative traces

Purified water level

3. Hair Shampoo Manufacturer

TYMP Protein 5.8 wt%

Sodium Laures Sulfate 35% by weight

1% by weight glycerin

Lauramid Die 3.5 wt%

1% by weight of propylene glycol

1% by weight of cocaamidopropyl betaine

Cetyl alcohol 0.1 wt%

0.5% by weight of glycol distearate

Butylene glycol 8% by weight

Methylparaben 0.2 wt%

0.1% by weight of triethanolamine

Citric Acid 0.1 wt%

Polyquaternium-7 0.2% by weight

Polycutternium-10 0.12 wt%

Olive Oil Fiji-7 Ester 0.2 wt%

A small amount of spices

Preservative traces

Purified water level

제제예 3. 식품 제제의 제조Formulation Example 3 Preparation of Food Formulation

1. Preparation of health food

TYMP protein 100 mg

Vitamin Mixture

70 g of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 g of vitamin B12

Vitamin C 10 mg

10 g of biotin

Nicotinamide 1.7 mg

Folic acid 50 g

Calcium Pantothenate 0.5 mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

15 mg potassium monophosphate

Dicalcium Phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

2. Manufacture of health drinks

TYMP protein 100 mg

15 g of vitamin C

100 g of vitamin E (powder)

Iron lactate 19.75 g

3.5 g of zinc oxide

Nicotinamide 3.5 g

0.2 g of vitamin A

0.25 g of vitamin B1

0.3 g of vitamin B2

Water quantification

After mixing the above components in accordance with a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Claims

A pharmaceutical composition for preventing or treating hair loss comprising TYMP (thymidine phosphorylase) protein as an active ingredient.
The method of claim 1,

The TYMP protein is characterized in that consisting of the amino acid represented by SEQ ID NO: 1, hair loss prevention or treatment pharmaceutical composition.
The method of claim 1,

The TYMP protein is characterized in that encoded by the polynucleotide of SEQ ID NO: 2, hair loss prevention or treatment pharmaceutical composition.
The method of claim 1,

The alopecia is characterized in that at least one selected from the group consisting of male hair loss, female hair loss, circular hair loss and resting hair loss, hair loss prevention or treatment pharmaceutical composition.
A quasi-drug composition for preventing or improving hair loss comprising TYMP protein as an active ingredient.
The method of claim 5,

The quasi-drug is one having at least one formulation selected from the group consisting of creams, lotions, aerosols, shampoos, gels and packs, qualitative composition for preventing or improving hair loss.
Hair loss prevention or improvement cosmetic composition containing TYMP protein as an active ingredient.
The method of claim 7, wherein the cosmetic composition is a hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder gel, hair pack, hair treatment, eyebrow hair regrowth, eyelash hair regrowth And eyelash nutrient having one or more formulations selected from the group consisting of, hair loss prevention or cosmetic composition for improvement.
A food composition for preventing or improving hair loss comprising TYMP protein as an active ingredient.
A pharmaceutical composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.
A quasi-drug composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.
Cosmetic composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.
Food composition for promoting hair growth or hair growth comprising TYMP protein as an active ingredient.
A method for preventing or treating hair loss, comprising: treating a subject with TYMP protein.
Treating the TYMP protein to the subject; Hair loss improvement method comprising a.
A method for promoting hair growth or hair growth comprising the step of treating the subject with TYMP protein.