WO2018020101A1 - Method for obtaining a unialgal biomass of cells of small multicellular macroalgae and endophytes of host macroalgae, and use of same in cosmetics - Google Patents

Abstract

Method for obtaining a unialgal biomass of small multicellular macroalgae cells and endophytes of host macroalgae, characterised in that it comprises the following successive steps: -A step A) of collecting, from the natural environment, a sample of said host macroalgae; -A step B) of preparing a unialgal sample of cells of said small multicellular macroalgae and endophytes of host macroalgae, by separating them from their macroalgae hosts, from the sample taken in step A) above; -A step C) of culturing said unialgal sample of small multicellular macroalgae cells and endophytes of host macroalgae obtained in step B), in seawater to which at least one nitrogen source has been added, to obtain an aqueous suspension of said unialgal biomass of small multicellular macroalgae cells; -A step D) of harvesting said unialgal biomass of small multicellular macroalgae cells from said aqueous suspension obtained at the end of step C). Glycolic extract of said biomass, method for preparing same, use of same in cosmetics and pharmacy and compositions containing it.

Description

 The present invention relates to a novel unialgular biomass extract of small-sized multicellular macroalgae cells, the method for obtaining a unialgal biomass of small-sized and endophytic macroalgae cells of macroalgae hosts, and its use in cosmetics. process for its preparation and its use as a cosmetic active, and cosmetic, pharmaceutical, dermopharmaceutical compositions for topical use comprising.

 Human skin is the first image offered in the eyes of others, and therefore, the improvement of its appearance is a subject of constant concern for human beings. The skin is a reflection of a state of well-being, often associated with youth, and conversely to a state of fatigue and / or aging. Skin aging is therefore a concern for humans and more particularly for consumers of cosmetic products who are looking for solutions to mitigate and / or prevent the visible manifestations related to aging. This cutaneous aging is observed at the level of the different cutaneous tissues and is characterized by metabolic, functional, cellular, architectural and tissue alterations, leading to visible external effects characterized by the appearance and increase of wrinkles, by a dull complexion. , by a lack of uniformity of the complexion (phenomenon of dyschromism), or by a modification of the texture and the properties, in particular biomechanical, of the skin of the human body.

Skin aging results from factors that are specific to each individual (characteristics of each individual's genetic heritage) and from environmental factors. Among the environmental factors that can cause skin aging include repeated and prolonged exposure to the sun, especially exposure to ultraviolet radiation, exposure to air pollution, cigarette smoke, various oxidative stress that may result among other factors previously mentioned, as well as psychological, emotional and nervous stress. The repeated and prolonged exposure of human skin to sunlight, and more particularly to ultraviolet radiation, leads to a form of aging that is commonly called photo-aging. This photo-aging is well documented in the scientific literature and it causes alterations of the skin at different levels, one of the most well-known skin alterations is solar elastosis, which is characterized by profound changes in the architecture and the organization of the elastic fibers of the dermis. These changes lead to a characteristic appearance of these skins which present very deep and marked wrinkles, inducing a tanned skin appearance, namely stiff, cracked and burnished, as well as modifications of their mechanical properties. Changes in the mechanical properties of human skin, related to aging, are due to the alteration of the dermal extracellular matrix, composed by elastic fibers and collagen fibers, and also to the alteration of cellular characteristics. Schulze et al. have shown that dermal fibroblasts stiffen with age, influencing cellular functions involving the cytoskeleton, such as contractile, migratory and proliferative properties, which are important for the reorganization of the extracellular matrix (Schulze et al., "Stiffening of human skin fibroblasts with age "Clin Plast Surg, 2012; 39 (1): 9-20).

 It is also known that reactive oxygen species (known as "ROS") in excess in human skin (whether the stimulus is exogenous or that production is endogenous) create irreversible binding with the identified proteins. under the term "carbonylated proteins", which then lose their function. There has recently been a link between these carbonyl proteins and their impact on key cellular functions such as carbohydrate metabolism, protein maintenance, cell mobility, including migration, and protein homeostasis [Baraibar and Friguet , "Oxidative proteome modifications target specifies cellular pathways during oxidative stress, cellular senescence and aging"; Exp. Gerontol. 2013; 48 (7): 620-5].

 At the cellular and tissue level, aging is characterized by several phenomena:

- In the epidermis, stem cells (CS) are undifferentiated cells that are characterized by their ability to maintain the integrity of an organ or rebuild it. They are most often located in anatomical "niches", which are associated with specific structural and functional properties. Adult skin contains stem cells that regulate its renewal, its surface appearance, and even its changes over time (Jones and Watt, "Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression" Cell (1993) May 21, 73 (4): 713-24). In the epidermis, the stem cells are located within the basal layer, their location being very specific. They represent 1 to 10% of the basal cells [Papini et al., "Isolation and clonal analysis of human epidermal cell keratinocyte stem cells in long-term culture", Stem Cells (2003) 21 (4): 481-94; Schneider et al., "Measuring stem cell frequency in epidermis: a quantitative in vivo functional assay for long-term repopulating cells", Proc. Natl. Acad. Sci. USA (2003) 30, 100 (20): 1412-7]. As a result of environmental factors, the asymmetric division of stem cells results in the generation of a transient amplifying daughter cell (TA, 40% of basal cells). The transient amplifying daughter cells are thus intended to proliferate, with a number of doublings of limited population, then become cells involved in a process of differentiation (post-mitotic cells), within the suprabasal layers [Jones and Watt, "Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression ", Cell. (1993) May 21; 73 (4): 713-24; Papini et al., "Isolation and clonal analysis of human epidermal keratinocytes stem cells in long-term culture", Stem Cells (2003) 21 (4): 481-94; Marconi et al., "Survivin identified keratinocyte stem cells and is down-regulated by anti-beta 1 integrin during anoikis", Stem Cells (2007) 25 (1): 149-55). During aging, the number of epidermal stem cells is decreased and their functions are altered (Papini et al., 2003 see above reference; Kwon et al., "Photoaging-associated changes in epidermal proliferative cell fractions in vivo", Dermatol Res (2008), 300 (1): 47-52).

 - In the dermis, many changes occur during aging. Collagens are particularly affected leading to disorganization and weakening of the collagen network. The cell-matrix attachment points become rarer, thus helping to reduce the interactions between the fibroblasts and the matrix. The response induced by the collagen fibers is thus reduced under the tension applied to the MEC. An overall decrease in the response to mechano-chemical stimuli is observed. In addition, the level of proteoglycans also decreases and the elastic fibers become fragmented (Silver et al., "Role of Mechanophysiology in Aging of ECM: effects of changes in mechanochemical transduction", J Appl Physiol Nov. 2003; 95: 2134- 41).

 Algae are chlorophyll photosynthetic organisms living in water or very humid environments. They can develop in seawater, freshwater, brackish water, in stagnant, beaten or turbulent environments. The algae can be unicellular or multicellular, they can be brown, green or red and are classified according to cytological and biochemical characteristics. These organisms play an important role on a global scale because they form the basis of food webs and they are involved in the production of atmospheric oxygen and in the fixation of carbon dioxide.

 Macroalgae are eukaryotic multicellular algae, usually visible to the naked eye and often referred to as macrophytes. They can reproduce sexually or asexually; in some species these two modes of reproduction succeed each other during the life cycle. The life cycle can include a generation, an alternation of two generations or three generations. Monogenetic, digenetic or trigenetic cycles will be discussed respectively.

Depending on the type of life cycle, the species may be in the form of gametophytes, sporophytes, carposporophyte or tetrasporophyte. During a cycle, these different forms of life can be either of the same morphologies so isomorphous, or of different morphologies so heteromorphic.

 The difference in morphology can be very significant, making it impossible to attach two life forms to the same species for an uninformed person. This difference can also be marked by the size of the seaweed, a life form can be macroscopic and another form of life can be microscopic. For example, the sporophyte of Undaria pinnatifida is nearly a meter long whereas the gametophyte of this alga is a few tens of micrometers.

 There are about two thousand species of brown algae, seven thousand species of red algae and one thousand seven hundred species of green algae.

 Macroalgae measure from a few tens of micrometers in length in the case of algae of the genus Acrochaetium to a hundred meters for the species Macrocystis py ri will.

 According to the World Marine Species Registry (WoRMS), in the taxonomic hierarchy of the plant kingdom, the Chlorophyceae class, identified as serial number (AphialD): 802, is part of the chlorophyte (green algae) division (AphialD: 801). This class of chorophyceae. Green algae contain pigments found in other plants such as chlorophylls a and b. They are at the origin of the appearance of terrestrial plants.

 Green algae are used in cosmetic preparations for different activities. The Korean patent published under number KR 10 135 653 B1 discloses a cosmetic composition comprising from 0.001 to 30% by weight of an Ulva pertusa hydrolysate (AphialD: 21 1460) as active and 1.0% of glucuronic acid . The international application published under the number WO 2015/071502 A1 discloses an algae extract of the order of Ulvales, containing sulphated and non-sulphated anionic polysaccharides of size less than 50 kDa, which are used as therapeutic agents to prevent and / or treat bacterial infections. More generally Vera et al. discloses the use of algae extracts of the genus ulva linnaeus, (AphialD: 144296), such as ulva rigida (AphialD: 145990) and ulva compressa (AphialD: 234462), comprising polysaccharides of molecular weight between 50 and 60kDa as antibacterial agent (Vera et al., Marin Drugs, Vol.9, No. 12, December 201 1, pp 2514-2525).

Of the ten thousand different species of macroalgae, only about one hundred of them now have an economic interest. Thus, some dietary macroalgae, hydrocolloid producing algae and algae producing biologically active molecules have been the subject of extensive research to better understand their physiology, metabolism and reproduction. Algae used in human nutrition like Undaria pinnatifida better known under the name of Wakame, Laminaria japonica also called Kombu, Porphyra yezoensis also called Nori and Enteromorpha intestinalis also called Ao Nori have been studied to control their culture.

 Hydrocolloids produced by algae like Chondrus crispus,

Kappaphycus spp. and Eucheuma denticulatum are mainly used as thickeners and gelling agents in food and cosmetics. The biomasses producing these polymers are of economic interest [Ronelie C. et al. Non-enzymatic isolation of somatic cells from Kappaphycus spp. and Eucheuma denticulatum (Solieriaceae, Rhodophyta). Eur. J. Phycol. (2014), 49 (4): 486-492].

 The bioreactor culture of the green alga Acrosiphonia coalita, the brown alga Laminaria saccharina and the red algae Agardhiella subulata, Ochtodes secundiramea and Portieria hornemannii have been studied with the aim of synthesizing molecules with biological activity [Gregory L. Rorrer et al . Production of bioactive metabolites by cell and tissue cultures of marine macroalgae in bioreactor Systems. Plant Cell and Tissue Culture for the Production of Food Ingredients, edited by Fu et al. Kluwer Academy / Plenum Publishers, New York, 1999].

 Some of them are based on knowledge and control of the reproduction cycle of the macroalga. For example, in laminariales alternation can be observed between a diploid sporophyte developed in macroscopic thallus and microscopic male and female haploid gametophytes. Fertile mature sporophytes produce swimming spores that settle on a solid substrate and give rise to gametophytes. The study of their life cycle as that of Undaria pinnatifida better known under the name of Wakame or Laminaria japonica called Kombu allowed to develop the culture of gametophytes, form of microscopic life of these algae, to seed supports on which will develop macroscopic sporophytes valued in human nutrition. The cultivation of these algae is first done in the laboratory for the culture of the microscopic life form and then in the natural environment to produce the macroscopic life form that can be consumed.

The development of sporophyte cultivation of brown algae of the order Laminariales such as Undaria pinnatifida in the 1980s led to the development of a gametophyte culture technique called "free living", which consists in harvesting sporophytes mature, cause sporulation, capture spores, form gametophytes and then cultivate them in order to create gametes that, after fertilization, will give rise to new sporophytes (R. Perez et al., 1984). The development of the sporophytic culture of Laminaria saccharina also required the development of a culture method of its gametophyte, similarly to the cultures of Undaria pinnatifida [C. Zhi, GL Rorrer. Photolithotrophic cultivation of Laminaria saccharina gametophyte cells in a bubble-column bioreactor. Enzyme and Microbial Technology. Volume 18, Issue 4, March 1996, Pages 291-299].

 The Chinese patent application published under the number CN 103858745 A, discloses the development of artificial Sytosiphon lomentaria cultures by controlling the steps of differentiation of the germinal plasma of the alga, to produce a mature monocular sporangia, then cause its sporulation for the purpose. to inoculate culture supports for producing at sea, Sytosiphon lomentaria macroscopic biomass.

 The Chinese patent application published under the number CN 103931482 A, discloses a method for obtaining a thallus of the gametophyte of the red algae Porphyra yezoensis, which is used in human nutrition, especially for the making of makis, involving first the culture "In vitro" of the conchocelis phase of the algae to produce conchospores that will be fixed on a culture support to allow the growth of thalli at sea.

 Other methods involve the induction of callus from explants of red algae, said callus resulting in the development of seedlings which are then grown in a bioreactor. In this context, the work of Ronélie et al. [Ronelie C. et al. Non-enzymatic isolation of somatic cells from Kappaphycus spp. and Eucheuma denticulatum (Solieriaceae, Rhodophyta), Eur. J. Phycol. (2014), 49 (4): 486-492]; those of J. Munoz [J. Munoz. Use of plant growth regulators in micropropagation of Kappaphycus alvarezii (Doty) in airlift bioreactors. J Appl Phycol (2006) 18: 209-218], or those of Maliakal et al. [S. Maliakal, D. Cheney. Halogenated monoterpenes production in regenerated plantlet cultures of Ochtodes secundiramea. J. Phycol. 37, 1010-1019 (2001)].

 Other methods involve the production of protoplasts from a thallus for the purpose of reforming new thalli, using, as proposed by Rusig et al. in the case of Enteromorpha intestinalis (TSN 6535), an algae used in food and feed, an enzymatic mixture containing cellulase and aplysia enzymes is used to digest the wall of the thallus cells of the algae. The cells whose wall is digested are called protoplasts [A Rusig & J. Cosson, Plant regeneration from protoplasts of Enteromorpha intestinalis (Chlorophyta, Ulvophyceae) as seedstock for macroagal culture. Journal of Applied Phycology 13: 103-108, 2001].

Since small-sized multicellular macroalgae are not abundant enough in the wild to allow harvesting in the environment and that their small size makes it difficult to identify the desired species as well as the specific harvest of a given species, the inventors have focused on developing a new method for obtaining a unialgal biomass of macroalgae cells of multicellular species. small size, in order to extract an active ingredient used in cosmetics.

 They have developed a method that can be used in a bioreactor, which does not require axenic explants and does not involve phytohormones or enzymes.

 According to a first aspect, the subject of the invention is a process for obtaining a unialgal biomass of small and endophytic macroalgae macroalgae cells of macroalgae, characterized in that it comprises the following successive stages:

 A step A) of sampling the natural environment from a sample of said host macroalgae;

 A step B) of preparing a unialgal sample of cells of said small-sized and endophytic macroalgae of macroalgae hosts, by their separation from their said macroalgae hosts, from the sample taken in step A ) previous ;

 A step C) of culturing said unialgal sample of small and endophytic macroalgae cells of macroalgae of macroalgae hosts isolated at the end of step B), in sea water supplemented with at least one source nitrogen, to obtain an aqueous suspension of said unialgal biomass of small multicellular macroalgae cells;

 A step D) of harvesting said unialgal biomass of small-sized multicellular macroalgal cells from said aqueous suspension obtained at the end of step C).

 By small-sized multicellular macroalga, is denoted in the method as defined above, a multicellular macroalga, measuring between 30μηι and 3cm and organized into cell clusters. This small-sized multicellular macroalga is differentiated from a large-sized multicellular macroalga because the latter is between 5 cm and 200 cm and is organized in a tissue way.

 By unialgal sample of multicellular macroalgae cells is meant in the process as defined above, a culture containing only one species of multicellular algae.

As a sample of a macroalga taken from the natural environment, implemented in stage A) of the process as defined above, a particular sample taken from seawater, in particular a sample of seawater, a sample taken from the surface of solid substrates such as rocks, sand, shells, sediments or artificial supports such as a boat hull, a pontoon or a dike; it can also be a sample taken from the surface or inside other plants (epiphyte or endophyte) such as algae or marine plants, on the surface or inside of animals (epiphyte or endophyte ) such as sponges, cnidarians, procordates, echinoderms, molluscs, arthropods, annelids, or marine vertebrates.

 The macroalgal sample taken from the natural environment, implemented in step A) of the process as defined above, is generally very rich in biodiversity and contains a wide selection of living organisms such as small animals, protozoa, prokaryotic microalgae, eukaryotic microalgae and multicellular macroalgae.

 According to step A) of the method as defined above, the unialgal sample of small multicellular macroalgae cells is obtained by isolating the target macroalgae from other organisms. For this purpose, it is possible to implement physical separation means and / or chemical separation means.

 As a means of physical separation, there is for example the separation carried out by means of a glass pipette using the end of the capillary to cut out some small sized multicellular macroalgae cells, controlling the operation visually, under a microscope or under a binocular loupe. There is also the separation by successive dilution of the cells of the target species from the natural sample.

 As a means of chemical separation, there is for example the use of antibiotics to eliminate microalgae of the cyanobacterial type, or that of germanium dioxide to eliminate microalgae of the diatom type.

The different means of isolation are combined to obtain the best possible isolation result. All isolation methods, physical and chemical, are carried out in translucent light-transmitting receptacles, containing sterile seawater, containing at least one source of nitrogen such as sodium nitrate (NaN0 ₃ ) to a concentration of between 50mg / dm ³ and 250mg / dm ³ with a preference for 150mg / dm ³ and a phosphorus source such as sodium dihydrogen phosphate (NaH ₂ PO ₄ ) at a concentration of between 5mg / dm ³ and 75mg / dm ³ with a preference for 50mg / dm ³ .

It is also possible to add other mineral elements to the seawater, for example by adding a nutritive medium in the desired proportions, such as the Provasoli medium of the following composition:

The implementation of step B) of the process as defined above is generally carried out by operating the following successive steps:

 At least one step B1) during which the host macroalgae isolated at the end of step A) are cut into a fragment of two to five centimeters in length, more particularly three centimeters in length;

 - At least one step B2) of cleaning said fragments obtained at the end of step B1), by physical action with absorbent paper with an aqueous solution comprising about 0.1% to 0.5 % by weight of nonionic surfactant, oil-in-water type, and more particularly of a sorbitan ester such as polyethoxylated sorbitan oleate with 20 moles of ethylene oxide or 20% polyethoxylated sorbitan stearate. moles of ethylene oxide, to eliminate a maximum of epiphyte contaminants;

 - At least one step B3), during which the fragments cleaned at the end of step B2) are rinsed in sterile seawater;

 - At least one step B4), said fragments cleaned and rinsed at the end of step B3), are cut longitudinally so as to promote direct contact of macroalgae of small size and endophytes of said macroalgae hosts with the external environment ;

At least one step B5), of chemical separation of the endophytes from their host, by bathing the fragments of algae cut out during step B4) in a medium composed of sterile seawater comprising at least one source of nitrogen and 1 mg / l of germanium dioxide, ie in a medium composed of sterile seawater containing at least one nitrogen source and an antibiotic mixture composed of ampicillin 50mg / l, streptomycin 50mg / l, and chloramphenicol 25mg / l.

 Sterile seawater means that the seawater used in the steps described above, has been autoclaved at 121 ° C for twenty minutes, in order to eliminate any microorganisms present.

 By nitrogen source is meant in particular an ammonium salt, an alkali or alkaline earth nitrate, or a mixture of these salts.

Stage C) of the process as defined above, is carried out in a photobioreactor containing seawater containing at least one nitrogen source such as sodium nitrate (NaN0 ₃ ) at a concentration of between 50 mg / dm ³ and 250mg / dm ³ with a preference for 150mg / dm ³ and a phosphorus source such as sodium dihydrogen phosphate (NaH ₂ PO ₄ ) at a concentration of between 5mg / dm ³ and 75mg / dm ³ with a preference for 50mg / dm ³ .

 It is also possible to add other mineral elements to the seawater by addition, for example, in the desired proportions of Provasoli's medium.

 The cultures are conducted in translucent culture tanks under air bubbling optionally enriched with carbon dioxide.

 The cultures are generally conducted at 10 ° C to 25 ° C with a preference for 17 ° C under constant illumination.

Cultures are conducted over periods of a fortnight in volumes ranging from 500cm ³ for the early stages of culture to 20m ³ for industrial biomass production steps. However, when binocular and / or microscope observations made at the end of the first fifteen-day period reveal the growth of other algae than that referred to at the end of step A), this is a sign that the result of step A) and / or that of step B) is not satisfactory. One or the other or all of these steps A) and B) of the process as defined above, must therefore be repeated until a unialgal sample of small-sized multicellular macroalgal cells is obtained. satisfactory quality.

Stage D) of the process as defined above is generally carried out using a filter cloth with a cut-off threshold of between 25 μm and 100 μm, depending on the size of the small-sized multicellular macroalgae grown in culture. Unialgal biomass culture of small-sized multicellular macroalgae cells is filtered, with seawater passing through the web and remaining biomass at its surface. The biomass retentate is then pressed to remove the free water still present. According to a particular aspect of the method as defined above, said small-sized and endophytic macroalgae of macroalgae hosts, are green algae from the class Ulvophyceae, the order of the Ulvales, the family of Ulvellaceae, the Ochlochaete genus and Ochlochaete hystrix species (AphialD: 146012, according to the World Marine Species Registry (WoRMS)).

 According to another particular aspect of the process as defined above, said macroalgae hosts are red algae from the class of Floridaophyceace, the order Ceramiales, family Rhodomelaceae, genus Osmundea and species Osmundea hybrida. (AphialD: 144842 according to the World Marine Species Registry (WoRMS)

 According to another particular aspect of the process as defined above, it further comprises a step E) of preparing a powder of said unialgal biomass of small-sized multicellular macroalgae cells obtained in step D).

 Step E) of the process as defined above is carried out by methods known to those skilled in the art. For example, it is possible to dry said unialgal biomass of small-sized multicellular macroalgae cells obtained in step D) of the process as defined above, and then grind and sieve it to obtain a powder of the desired average diameter.

 According to a particular aspect, during step E) of the process as defined above, said unialgal biomass of small-sized multicellular macroalgae cells obtained in step D) is frozen, freeze-dried and then milled to obtain the desired powder.

 The subject of the invention is also a method for preparing a glycolic extract of said unialgal biomass of small-sized and endophytic macroalgae macroalgae host cells, obtained by the process as defined above, characterized in that it comprises the following successive steps:

 A step F) during which the powder of said unialgal biomass of small-sized multicellular macroalgae cells obtained in step D) or in step E) of the process as defined above, is dispersed with stirring in a water-glycol mixture at a rate of 0.2% by mass at 20% by mass and more particularly from 0.2% to 10% by mass, of biomass per 100% by mass of dispersion;

 - A step G) during which the dispersion obtained in step F) above, is separated into its immiscible phases, to obtain the expected glycolic extract.

As glycol used in the process as defined above, there are in particular 1,3-propanediol, butylene glycol or 1,4-butanediol, 1,3-butanediol, 1,2-butanediol, 1,2-pentanediol, 1,5-pentanediol, 1,2-hexanediol or 1,6-hexanediol. According to a particular aspect of the process as defined above, the glycol used is 1,3-propanediol.

 During step F) of the process as defined above, the mass proportion of the glycol in the water-glycol mixture used in the process as defined above, is generally greater than or equal to 10% by mass and lower or equal to 100% by mass, more particularly greater than or equal to 25% by mass and less than or equal to 75% by mass. If necessary or if desired after maintaining stirring of the mixture for one to two hours, this mass ratio is adjusted by adding water at the end of this step F) so that the mass content of 1,3-propanediol between 40% and 60%.

 The subject of the invention is also a glycolic extract of a unialgal biomass of small and endophytic macroalgae macroalgae cells obtained by the process comprising the following successive steps:

 A step A) of sampling the natural environment from a sample of said host macroalgae;

 A step B) of preparing a unialgal sample of cells of said small-sized and endophytic macroalgae of macroalgae hosts, by their separation from their said macroalgae hosts, from the sample taken in step A ) previous ;

 A step C) of culturing said unialgal sample of small and endophytic macroalgae macroalgae cells of macroalgae obtained in step B), in sea water with at least one nitrogen source, to obtain an aqueous suspension of said unialgal biomass of small-sized multicellular macroalgae cells;

 A step D) of harvesting said unialgal biomass of small-sized multicellular macroalgal cells from said aqueous suspension obtained at the end of step C);

 - Optionally a step E) of preparing a powder of said unialgal biomass of small multicellular macroalgae cells obtained in step D);

 A step F) during which said unialgal biomass of small-sized multicellular macroalgal cells obtained in step D) or in step E) is dispersed with stirring in a water-glycol mixture at a rate of 0, 2% by mass at 20% by weight and more particularly from 0.2% to 10% by mass of biomass per 100% by mass of dispersion;

- A step G) during which the dispersion obtained in step F) above, is separated into its immiscible phases, to obtain the expected glycolic extract. According to a particular aspect of the present invention, the subject of the present invention is the glycolic extract as defined above, in which the glycol used is 1,3-propanediol.

 According to another particular aspect of the present invention, the subject of the present invention is the glycolic extract as defined above, characterized in that the powder of said unialgal biomass of small-sized multicellular macroalgae cells is used at the same time. step E) of the process for its preparation, is a powder of said unialgal biomass of green algae cells from the class Ulvophyceae, the order Ulvales, the family Ulvellaceae, the genus Ochlochaete and the species Ochlochaete hystrix.

 According to a more particular aspect of the present invention, this relates to the glycolic extract as defined above, characterized in that during step F) of the process for its preparation:

 the water-glycol mixture used is a water-1,3-propanediol mixture whose mass content in 1,3-propanediol (ratio of mass 1,3-propanediol to total mass 1,3-propanediol and water) is included between 25% and 100%,

 the dispersion in said water-glycol mixture is carried out in a proportion of 0.2% to 10% by mass of biomass per 100% by mass of dispersion; and

 the stirring is maintained for one to two hours, then if necessary or if desired,

 the mass content of 1,3-propanediol is adjusted between 40% and 60%.

 The subject of the invention is also the use of the glycolic extract of a unialgal biomass of small-sized multicellular macroalgae cells as defined above, as an anti-aging cosmetic active ingredient, and more particularly the use thereof. as defined above in which the glycolic extract of a unialgal biomass of small multicellular macroalgae cells is the glycolic extract of a unialgal biomass of green algal cells from the class of Ulvophyceae, the order of Ulvales, family Ulvellaceae, genus Ochlochaete and species Ochlochaete hystrix, as defined above.

The invention also relates to a cosmetic composition (C1) for topical use comprising at least one cosmetically acceptable excipient and an effective amount of the glycolic extract of a unialgal biomass of small-sized multicellular macroalgae cells as defined above. above, and more particularly a composition (C1) as defined above in which the glycolic extract of a unialgal biomass of small multicellular macroalgae cells, is the glycolic extract of a unialgal biomass of cells of green algae from the class Ulvophyceae, the order Ulvales, family Ulvellaceae, genus Ochlochaete and species Ochlochaete hystrix as defined above. In the method as defined above, said composition (C1) is generally spread on the surface of the skin to be treated, then the skin is massaged a few moments.

 The expression "for topical use" used in the definition of the composition (C1) that is the subject of the present invention means that said composition (C1) is implemented by application to the skin, whether it be a direct application or indirect application when said composition (C1) according to the invention is impregnated on a support intended to be brought into contact with the skin (paper, wipe, textile, transdermal device, etc.).

 The expression "cosmetically acceptable" used in the definition of the composition (C1) object of the present invention, means according to the directive of the Council of the European Economic Community N ° 76/768 / CEE of July 27, 1976 modified by the directive N 93/35 / EEC of 14 June 1993, that it includes any substance or preparation intended to be placed in contact with the various parts of the human body (epidermis, hair and hair system, nails, lips and genitals) or with the teeth and oral mucosa in view, exclusively and mainly, to clean, perfume, modify the appearance and / or correct body odor and / or protect or maintain them in good condition .

 By effective quantity of the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells as defined above, the term "100% of the mass of said composition (C1)" is understood to mean a quantity of between 0.1% and 5%. % by mass, more particularly between 0.1% and 3% by weight, and even more particularly between 0.5% and 2% by weight of said glycolic extract of said unialgal biomass of small multicellular macroalgae cells.

 The composition (C1) which is the subject of the present invention is generally in the form of an aqueous or hydroalcoholic or hydro-glycolic solution, in the form of a suspension, an emulsion, a microemulsion or a nano-emulsion, whether water-in-oil, oil-in-water, water-in-oil-in-water or oil-in-water-in-oil, or in the form of a powder.

 The composition (C1) which is the subject of the present invention may be packaged in a bottle, in a device of the "bottle" pump type, in pressurized form in an aerosol device, in a device provided with a perforated wall such as a grid or in a device provided with a ball applicator (called "roll-on").

In general, the glycolic extract of said unialgal biomass of small-sized multicellular macroalgal cells which is the subject of the present invention is associated with chemical additives usually used in the field of formulations for topical use, such as foaming surfactants and detergents, thickening and / or gelling surfactants, thickening and / or gelling agents, agents stabilizers, film-forming compounds, solvents and co-solvents, hydrotropic agents, thermal or mineral waters, plasticizers, emulsifiers and co-emulsifiers, opacifying agents, pearlescent agents, superfatting agents, sequestering agents, chelating agents, oils, waxes, antioxidants, perfumes, essential oils, preservatives, conditioners, deodorants, bleaching agents for hair and skin discoloration, active ingredients for to provide a treating and / or protective action with respect to the skin or hair, sunscreens, mineral fillers or pigments, particles providing a visual effect or intended for the encapsulation of active ingredients, exfoliating particles, texture agents, optical brighteners, repellents for insects.

 Examples of foaming and / or detergent surfactants that can be combined with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgal cells in the composition (C1) include anionic foaming and / or detergent surfactants. , cationic, amphoteric or nonionic.

 Among the foaming and / or detergent anionic surfactants, mention may be made of the alkali metal, alkaline earth metal, ammonium, amine or alkylalcohol salts of alkylether sulfates, alkyl sulphates, alkylamidoethers sulphates, alkylaryl polyether sulphates, monoglyceride sulphates, alpha-olefin sulphonates, paraffin sulphonates, alkyl phosphates, alkyl ether phosphates, alkyl sulphonates, alkylamide sulphonates, alkylaryl sulphonates, alkyl carboxylates alkyl sulfosuccinates, alkyl ether sulfosuccinates, alkylamide sulfosuccinates, alkyl sulfoacetates, alkyl sarcosinates, acyl isethionates, N-acyl taurates, acyl lactylates, N-acyl derivatives of amines, N-acyl derivatives of peptides, N-acyl derivatives of proteins, N-acyl derivatives of fatty acids.

 Amphoteric foaming and / or detergent surfactants include alkylbetaines, alkylamidobetaines, sultaines, alkylamidoalkylsulfobetaines, imidazoline derivatives, phosphobetaines, amphopolyacetates and amphopropionates.

 Among the foaming and / or detergent cationic surfactants, mention may be made especially of quaternary ammonium derivatives.

Among the foaming and / or detergent nonionic surfactants, mention may be made more particularly of alkylpolyglycosides comprising a linear or branched, saturated or unsaturated aliphatic radical containing from 8 to 16 carbon atoms, such as octyl polyglucoside or decyl polyglucoside. undecylenyl polyglucoside, dodecyl polyglucoside, tetradecyl polyglucoside, hexadecyl polyglucoside, 1-12-dodecanediyl polyglucoside; ethoxylated hydrogenated castor oil derivatives such as the product marketed under the INCI name "Peg-40 hydrogenated castor oil"; the polysorbates such as Polysorbate 20, Polysorbate 40, Polysorbate 60, Polysorbate 70, Polysorbate 80, Polysorbate 85; coconut amides; N-alkylamines.

 Examples of thickening and / or gelling surfactants that may be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include fatty esters of alkylpolyglycosides which may be alkoxylated. such as ethoxylated methylpolyglucoside esters such as PEG 120 methyl glucose trioleate and PEG 120 methyl glucose dioleate respectively marketed under the names GLUCAMATE ™ LT and GLUMATE ™ DOE120; alkoxylated fatty esters such as PEG 150 pentaerythrityl tetrastearate marketed under the name CROTHIX ™ DS53, PEG 55 propylene glycol oleate sold under the name ANTIL ™ 141; fatty chain polyalkylene glycol carbamates such as PPG-14 laureth isophoryl dicarbamate marketed under the name ELFACOS ™ T21 1, PPG-14 palmeth-60 hexyl dicarbamate sold under the name ELFACOS ™ GT2125.

As examples of thickening and / or gelling agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgal cells in the composition (C1), mention may be made of linear polyelectrolyte polymers. or branched or cross-linked, such as homopolymer of partially or totally salified acrylic acid, homopolymer of partially or totally salified methacrylic acid, homopolymer of 2-methyl-[(1-oxo-2) partially or completely salified, copolymers of acrylic acid and AMPS, copolymers of acrylamide and AMPS, copolymers of vinylpyrrolidone and AMPS, copolymers of AMPS and (2-hydroxyethyl) acrylate, copolymers of AMPS and (2-hydroxyethyl) methacrylate, copolymers of AMPS and hydroxyethylacrylamide, copolymers of AMPS and Ν, Ν-di methyl acrylamide, copolymers of AMPS and tris (hydroxymethyl) acrylamido methane (THAM), copolymers of acrylic or methacrylic acid and (2-hydroxyethyl) acrylate, copolymers of acrylic or methacrylic acid and (2-hydroxyethyl) methacrylate, copolymers of acrylic or methacrylic acid and hydroxyethylacrylamide, copolymers of acrylic or methacrylic acid and THAM, copolymers of acrylic acid or methacrylic acid and Ν, Ν-dimethyl acrylamide, terpolymers of acrylic or methacrylic acid, AMPS and (2-hydroxyethyl) acrylate, terpolymers of acrylic or methacrylic acid, AMPS and (2-hydroxyethyl) methacrylate, terpolymers of acrylic or methacrylic acid, AMPS and THAM, terpolymers of acrylic or methacrylic acid, AMPS and N, N dimethyl acrylamide, terpolymers of acrylic or methacrylic acid, AMPS and acrylamide, copolymers of acrylic acid or methacrylic acid and alkyl acrylates whose carbon chain comprises between four and thirty carbon atoms and more particularly between ten and thirty carbon atoms, copolymers of AMPS and alkyl acrylates whose carbon chain comprises between four and thirty carbon atoms and more particularly between ten and thirty carbon atoms the linear, branched or crosslinked terpolymer of at least one monomer having a strong, free, partially salified or totally salified acidic function, with at least one neutral monomer, and at least one monomer of formula (VIII):

CH ₂ = C (R '3) -C (= O) - [CH ₂ -CH ₂ -O] _n -R' ₄ (VIII)

in which R ' ₃ represents a hydrogen atom or a methyl radical, R' ₄ represents a linear or branched alkyl radical containing from eight to thirty carbon atoms and n represents a number greater than or equal to one and less than or equal to fifty .

 The polyelectrolytic polymers, linear or branched or crosslinked that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), can be in the form of a solution, an aqueous suspension, a water-in-oil emulsion, an oil-in-water emulsion, a powder. The polyelectrolytic polymers, linear or branched or crosslinked that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), can be selected from the products marketed under the SIMULGEL ™ EG, SIMULGEL ™ EPG, SEPIGEL ™ 305, SIMULGEL ™ 600, SIMULGEL ™ NS, SIMULGEL ™ INS 100, SIMULGEL ™ FL, SIMULGEL ™ A, SIMULGEL ™ SMS 88, SEPINOV ™ EMT 10, SEPIPLUS ™ 400, SEPIPLUS ™ 265, SEPIPLUS ™ S, SEPIMAX ™ Zen, ARISTOFLEX ™ AVC, ARISTOFLEX ™ AVS, NOVEMER ™ EC-1, NOVEMER ™ EC 2, ARISTOFLEX ™ HMB, COSMEDIA ™ SP, FLOCARE ™ AND 25, FLOCARE ™ AND 75, FLOCARE ™ AND 26, FLOCARE ™ AND 30, FLOCARE ™ AND 58, FLOCARE ™ PSD 30, VISCOLAM ™ AT 64, VISCOLAM ™ AT 100.

As examples of thickening and / or gelling agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of polysaccharides consisting solely of such as glucose glucans or homopolymers, glucomannoglucans, xyloglycans, galactomannans whose degree of substitution (DS) of D-galactose units on the main D-mannose chain is between 0 and 1, and more particularly between 1 and 0.25, such as galactomannans derived from cassia gum (DS = 1/5), locust bean gum (DS = 1/4), tara gum (DS = 1/3), guar gum (DS = 1/2), fenugreek gum (DS = 1).

 As examples of thickening and / or gelling agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of polysaccharides consisting of oste, such as sulphated galactans and more particularly carrageenans and agar, uronanes and more particularly alginines, alginates and pectins, heteropolymers of oste and uronic acids and more particularly xanthan gum, gellan gum , exudates of arabic gum and karaya gum, glucosaminoglycans.

 Examples of thickening and / or gelling agents that can be combined with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include cellulose, cellulose derivatives such as methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, silicates, starch, hydrophilic derivatives of starch, polyurethanes.

 Examples of stabilizing agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include microcrystalline waxes, and more particularly ozokerite, inorganic salts such as sodium chloride or magnesium chloride, silicone polymers such as polysiloxane polyalkyl polyether copolymers.

 As examples of solvents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of water, organic solvents such as glycerol, diglycerol, and the like. , oligomers of glycerol, ethylene glycol, propylene glycol, butylene glycol, 1,3-propanediol, 1,2-propanediol, hexylene glycol, diethylene glycol, xylitol, erythritol, sorbitol, water-soluble alcohols such as ethanol, isopropanol or butanol, water mixtures and said organic solvents.

As examples of thermal or mineral waters that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of thermal or mineral waters having a mineralization of at least 300 mg / l, in particular Avene water, Vittel water, the waters of the Vichy basin, Uriage water, Roche Posay water, water from Bourboule, the water of Enghien-les-bains, the water of Saint-Gervais-les bains, the water of Néris-les-bains, the water of Allevard-les-bains, the water of Digne , Maizieres water, Neyrac-les-bains water, Lons le Saunier water, Rochefort water, Saint Christau water, Fumades water and Tercist water the baths. As examples of hydrotropic agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of xylenesulfonates, cumenesulfonates and hexyl polyglucoside, (2-ethyl hexyl) polyglucoside or n-heptyl polyglucoside.

 Examples of emulsifying surfactants which may be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include nonionic surfactants, anionic surfactants, cationic surfactants.

 As examples of emulsifying nonionic surfactants which may be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of the esters of fatty acids and of sorbitol like the products marketed under the MONTANE ™ 40, MONTANE ™ 60, MONTANE ™ 70, MONTANE ™ 80 and MONTANE ™ 85 names; compositions comprising glycerol stearate and ethoxylated stearic acid between five moles and one hundred and fifty moles of ethylene oxide, such as the composition comprising stearic acid ethoxylated to one hundred and thirty-five moles of ethylene oxide and glycerol stearate marketed under the name SIMULSOL ™ 165; mannitan esters; ethoxylated mannitan esters; sucrose esters; methyl glucoside esters; alkyl polyglycosides having a linear or branched aliphatic radical, saturated or unsaturated, and having from fourteen to thirty six carbon atoms, such as tetradecyl polyglucoside, hexadecyl polyglucoside, octadecyl polyglucoside, hexadecyl polyxyloside, octadecyl polyxyloside, eicosyl polyglucoside, dodecosyl polyglucoside, (2-octyl dodecyl) polyxyloside, (12-hydroxy stearyl) polyglucoside; linear or branched fatty alcohol compositions, saturated or unsaturated, and containing from fourteen to thirty-six carbon atoms, and alkyl polyglycosides as described above, for example the compositions sold under the tradenames MONTANOV ™ 68, MONTANOV ™ 14, MONTANOV ™ 82, MONTANOV ™ 202, MONTANOV ™ S, MONTANOV ™ W018, MONTANOV ™ L, FLUIDANOV ™ 20X and EASYNOV ™.

 As examples of anionic surfactants that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of glyceryl stearate citrate, cetearyl sulphate, soaps such as sodium stearate or triethanolammonium stearate, N-acyl derivatives of salified amino acids such as stearoyl glutamate.

As examples of cationic emulsifying surfactants that can be associated with the glycolic extract of said unialgale macroalgae cell biomass multicellular small size in the composition (C1), there may be mentioned aminoxides, quaternium-82 and surfactants described in the patent application WO 96/00719 and mainly those whose fatty chain comprises at least sixteen carbon atoms.

 Examples of opacifying and / or nacrating agents that may be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include sodium palmitate, stearate of sodium, sodium hydroxystearate, magnesium palmitate, magnesium stearate, magnesium hydroxystearate, ethylene glycol monostearate, ethylene glycol distearate, polyethylene glycol monostearate, polyethylene glycol distearate the fatty alcohols having from twelve to twenty-two carbon atoms.

 As examples of texture agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgal cells in the composition (C1), mention may be made of N-acylated amino acid derivatives, such as lauroyl lysine marketed under the name AMINOHOPE ™ LL, octenyl starch succinate sold under the name DRYFLO ™, myristyl polyglucoside marketed under the name MONTANOV ™ 14, cellulose fibers, cotton fibers, fibers chitosan, talc, sericite, mica.

As examples of deodorant agents that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), there may be mentioned alkali silicates, zinc salts such as sulphate zinc, zinc gluconate, zinc chloride, zinc lactate; quaternary ammonium salts such as cetyltrimethylammonium salts, cetylpyridinium salts; glycerol derivatives such as glycerol caprate, glycerol caprylate, polyglycerol caprate; 1,2-decanediol; 1,3-propanediol; salicylic acid; sodium bicarbonate; cyclodextrins; metallic zeolites; TRICLOSAN ™; aluminum bromohydrate, aluminum chlorohydrates, aluminum chloride, aluminum sulphate, aluminum and zirconium hydrochlorides, aluminum and zirconium trihydrochloride, aluminum zirconium tetrachlorohydrate , aluminum and zirconium pentachlorohydrate, aluminum and zirconium octochlorhydrate, aluminum sulphate, sodium and aluminum lactate, aluminum and glycol hydrochloride complexes, as well as propylene glycol aluminum dihydrochloride complex, aluminum and propylene glycol sesquichlorohydrate complex, polyethylene glycol aluminum aluminum and polyethylene glycol, the complex of aluminum sesquichlorohydrate and polyethylene glycol. Examples of oils which can be combined with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include mineral oils such as paraffin oil, lemon Vaseline oil, isoparaffins or mineral white oils; oils of animal origin, such as squalene or squalane, vegetable oils, such as phytosqualane, sweet almond oil, coconut oil, castor oil, jojoba oil, olive oil, rapeseed oil, peanut oil, sunflower oil, wheat germ oil, corn germ oil, soybean oil, cottonseed oil, alfalfa oil, poppy oil, pumpkin oil, evening primrose oil, millet oil, barley oil, rye oil, Safflower oil, bancoulier oil, passionflower oil, hazelnut oil, palm oil, shea butter, apricot kernel oil, coriander seed oil, oil of oil, calophyllum oil, sysymbrium oil, avocado oil, calendula oil, oils derived from flowers or vegetables vegetable oils ethoxylated; synthetic oils such as fatty acid esters such as butyl myristate, propyl myristate, isopropyl myristate, cetyl myristate, isopropyl palmitate, octyl palmitate, butyl stearate, hexadecyl stearate, isopropyl stearate, octyl stearate, isocetyl stearate, dodecyl oleate, hexyl laurate, propylene glycol dicaprylate, lanolic acid derived esters, such as isopropyl lanolate, isocetyl lanolate, monoglycerides, diglycerides and triglycerides of fatty acids such as glycerol triheptanoate, alkylbenzoates, hydrogenated oils, poly (alpha-olefin), polyolefins such as polyisobutane ) synthetic isoalkanes such as isohexadecane, isododecane, perfluorinated oils; silicone oils such as dimethylpolysiloxanes, methylphenylpolysiloxanes, amine-modified silicones, fatty acid-modified silicones, alcohol-modified silicones, alcohol-modified silicones and fatty acids, modified silicones, polyether groups, modified epoxy silicones, silicones modified with fluorinated groups, cyclic silicones and silicones modified with alkyl groups. By "oils" is meant herein the compounds and / or the mixtures of water-insoluble compounds having a liquid appearance at a temperature of 25 ° C.

As examples of waxes that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of beeswax, carnauba wax and wax. candelilla, ouricoury wax, Japanese wax, cork fiber wax, sugar cane wax, paraffin waxes, lignite waxes, microcrystalline waxes, lanolin wax; ozokerite; polyethylene wax; silicone waxes; vegetable waxes; fatty alcohols and solid fatty acids at room temperature; glycerides solid to ambient temperature. By "waxes" is meant in the present application the compounds and / or mixtures of compounds insoluble in water, having a solid appearance at a temperature greater than or equal to 45 ° C.

As examples of active principles which can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of vitamins and their derivatives, in particular their esters, such as retinol (vitamin A) and its esters (retinyl palmitate for example), ascorbic acid (vitamin C) and its esters, ascorbic acid sugar derivatives (such as ascorbyl glucoside), tocopherol (vitamin E) and its esters (such as tocopherol acetate), vitamins B3 or B10 (niacinamide and its derivatives); compounds showing a lightening or depigmenting action of the skin, such as ω-undecylenoyl phenylalanine marketed under the name SEPIWHITE ™ MSH, SEPICALM ™ VG, the mono ester and / or the glycerol diester of ω-undecylenoyl phenylalanine, the ω- undecylenoyl dipeptides, arbutin, kojic acid, hydroquinone, the composition marketed under the brand name Seashine ™, cell culture of Crithmum maritimum sold under the trademark Celtosome ™ Crithmum maritimum; the compounds showing a soothing action including SEPICALM ™ S, allantoin and bisabolol; anti-inflammatory agents; compounds showing a moisturizing action such as urea, hydroxyureas, glycerol, polyglycerols, glycerolglucoside, diglycerolglucoside, polyglycerylglucosides, xylitylglucoside, the composition sold under the brand name AQUAXYL ™, the composition sold under the name of PRO-XYLANE ™ brand, derivatives of C-glycosides and more particularly derivatives of C-glucosides, of C-xylosides; extracts of Codium Tomentosum algae such as those included in the composition marketed under the brand name Codiavelane ™, extracts of seed products such as those included in the composition marketed under the brand name Bioplasma ™ (INCI name, Aqua- Buiyiene giyco! Piankton extract Ârginine ferruiate), plant extracts rich in polyphenols such as grape extracts, pine extracts, wine extracts, olive extracts; compounds showing a slimming or lipolytic action such as caffeine or its derivatives, ADIPOSLIM ™, ADIPOLESS ™, fucoxanthin, the composition marketed under the brand name Rhodysterol ™; N-acylated proteins; N-acylated peptides such as MATRIXIL ™; N-acyl amino acids; partial hydrolysates of N-acylated proteins; amino acids; peptides; total hydrolysts of protein; soy extracts, for example Raffermine ™; wheat extracts for example TENSINE ™ or GLIADINE ™; plant extracts, such as tannin-rich plant extracts, plant extracts rich in isoflavones or plant extracts rich in terpenes; freshwater or marine algae extracts; extracts marine plants; marine extracts in general such as corals; essential waxes; bacterial extracts; ceramides; phospholipids; compounds exhibiting antimicrobial action or purifying action, such as LIPACIDE ™ C8G, LIPACIDE ™ UG, SEPICONTROL ™ A5, FLUIDIPURE ™ 8G; OCTOPIROX ™ or SENSIVA ™ SC50; compounds showing an energizing or stimulating property such as PHYSIOGENYL ™, panthenol and its derivatives such as SEPICAP ™ MP; anti-aging actives like SEPILIFT ™ DPHP, LIPACIDE ™ PVB, SEPIVINOL ™, SEPIVITAL ™, MANOLIVA ™, PHYTO-AGE ™, TIMECODE ™; SURVICODE ™; anti-aging photo active ingredients; the active ingredients protecting the integrity of the dermal-epidermal junction; actives enhancing the synthesis of extracellular matrix components such as collagen, elastins, glycosaminoglycans; assets acting favorably on chemical cellular communication such as cytokines or physical ones such as integrins; active ingredients that create a sensation of "heating" on the skin, such as activators of cutaneous microcirculation (such as nicotinic acid derivatives) or products that create a sensation of "freshness" on the skin (such as menthol and derivatives) ; the active agents improving cutaneous microcirculation, for example the venotonic ones; draining assets; decongestant active ingredients such as extracts of ginko biloba, ivy, horse chestnut, bamboo, ruscus, holly, centalla asiatica, fucus, rosemary, willow; tanning agents or skin browning agents, for example dihydroxyacetone (DHA), erythrulose, mesotartaric aldehyde, glutaraldehyde, glyceraldehyde, alloxane, ninhydrin, plant extracts, for example wood extracts red Pterocarpus and Baphia genus such as Pteropcarpus santalinus, Pterocarpus osun, Pterocarpus soyauxii, Pterocarpus erinaceus, Pterocarpus indicus or Baphia nitida such as those described in the European patent application EP 0 971 683; agents known for their action of facilitating and / or accelerating the tanning and / or browning of human skin, and / or for their action of coloring human skin, for example caratenoids (and more particularly beta carotene) and gamma carotene), the product marketed under the tradename "Carrot oil" (INCI name: Daucus Carota, helianthus annuus sunflower oil) by Provital, which contains carotenoids, vitamin E and vitamin K; tyrosine and / or its derivatives, known for their effect on the tanning acceleration of human skin in association with exposure to ultraviolet radiation, for example the product marketed under the brand name "SunTan Accelerator ™" by the Provital company which contains tyrosine and riboflavins (vitamin B), the tyrosine and tyrosinase complex marketed under the brand name "Zymo Tan Complex" by Zymo Line, the product marketed under the brand name MelanoBronze ™ (INCI name: Acetyl Tyrosine, Monk's pepper extract (Vitex Agnus castus)) by the company Mibelle which contains acetyl tyrosine, product sold under the brand name Unipertan VEG-24/242/2002 (INCI name: Butylene Glycol and Acetyl Tyrosine and Hydrolyzed Vegetable Protein and Adenosine Triphosphate) by the company UNIPEX, the product sold under the trademark "Try-Excell ™" (INCI name: Oleoyl Tyrosine and Luffa Cylindrica (Seed) Oil and Oleic acid) by the company Sederma which contains extracts of pumpkin seed (or Loofah oil), the product marketed under the brand name "Actibronze ™" (INCI name: hydrolyzed wheat protein and acetyl tyrosine and copper gluconate) by the Alban Muller company, the product marketed under the brand name Tyrostan ™ (INCI name: potassium caproyl tyrosine) by Synerga, the product sold under the trade name Tyrosinol (INCI name: Sorbitan Isostea) spleen, glyceryl oleate, caproyl Tyrosine) by the company Synerga, the product sold under the brand name InstaBronze ™ (INCI name: Dihydroxyacetone and acetyl tyrosine and copper gluconate) marketed by Alban Muller, the product sold under the brand name Tyrosilane (INCI name: methylsilanol and acetyl tyrosine) by Exymol; peptides known for their effect of activating melanogenesis, for example the product marketed under the brand name Bronzing SF Peptide powder (INCI name: Dextran and Octapeptide-5) by the company Infinitec Activos, the product sold under the brand name Melitane (INCI name: Glycerin and Aqua and Dextran and Acetyl hexapeptide-1) including acetyl hexapeptide-1 known for its alpha-MSH agonist action, the product marketed under the brand name Melatimes Solutions ™ (INCI name: Butylene glycol, Palmitoyl Tripeptide-40) by LIPOTEC, sugars and sugar derivatives, for example the product marketed under the trade name Tanositol ™ (INCI name: inositol) by the company Provital, the product marketed under the name of brand Thalitan ™ (or Phycosaccharide ™ AG) by the company CODIF International (INCI name: Aqua and hydrolyzed algin (Laminaria Digitata) and magnesium sulfate and manganese sulfate) containing an oli gosaccharide of marine origin (guluronic acid and mannuronic acid chelated with magnesium and manganese ions), the product marketed under the trade name Melactiva ™ (INCI name: Maltodextrin, Mucuna Pruriens Seed extract) by the company Alban Muller, the rich compounds in flavonoids for example the product sold under the brand name "Biotanning" (INCI name: Hydrolyzed citrus Aurantium dulcis fruit extract) by the company Silab and known to be rich in flavonoids of lemon (hesperidin type); agents for the treatment of hair and / or hair, for example protective agents of the hair follicle melanocytes, for protecting said melanocytes against the cytotoxic agents responsible for the senescence and / or apoptosis of said melanocytes, such as the mimetics of the DOPAchrome tautomerase activity chosen from those described in the European patent application published under the number EP 1 515 688 A2, the synthetic mimetic molecules of SOD, for example the manganese complexes, antioxidant compounds, for example the cyclodextrin derivatives, silicic compounds derived from ascorbic acid, pyrrolidone carboxylate of lysine or arginine, associations of mono- and diester of cinnamic acid and vitamin C, and more generally those cited in the application for European patent published under the number EP 1 515 688 A2.

 Examples of antioxidants that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include EDTA and its salts, citric acid , tartaric acid, oxalic acid, BHA (butylhydroxyanisol), BHT (butylhydroxytoluene), tocopherol derivatives such as tocopherol acetate, mixtures of antioxidant compounds such as DISSOLVINE ™ GL 47S marketed by the Akzo Nobel company under the INCI name: Tetrasodium Glutamate Diacetate.

 Examples of sunscreens that can be combined with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1) include all those contained in the cosmetic directive 76/768 / EEC amended Annex VII.

Among the solar organic filters that can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of the family of benzoic acid derivatives such as the acids para-aminobenzoic acid (PABA), in particular the monoglycerol esters of PABA, the ethyl esters of Ν, Ν-propoxy PABA, the ethyl esters of Ν, Ν-diethoxy PABA, the ethyl esters of Ν, Ν-dimethyl PABA, the esters methyl N, N-dimethyl PABA, butyl esters of Ν, Ν-dimethyl PABA; the family of anthranilic acid derivatives such as homomenthyl-N-acetyl anthranilate; the family of salicylic acid derivatives such as amyl salicylate, homomenthyl salicylate, ethylhexyl salicylate, phenyl salicylate, benzyl salicylate, p-isopropanol phenyl salicylate; the family of cinnamic acid derivatives such as ethylhexyl cinnamate, ethyl-4-isopropyl cinnamate, methyl-2,5-diisopropyl cinnamate, p-methoxypropyl cinnamate, p-methoxyisopropyl cinnamate p-methoxyisoamyl cinnamate, p-methoxyoctyl cinnamate, (p-methoxy-2-ethylhexyl) cinnamate, (p-methoxy-2-ethoxyethyl) cinnamate, (p-methoxycyclohexyl) cinnamate, ethyl-ocyano-3-phenyl cinnamate, (2-ethyl hexyl) -cyano-3-phenyl cinnamate, glyceryl diparamethoxy mono-2-ethylhexanoyl cinnamate; the family of benzophenone derivatives such as 2,4-dihydroxy benzophenone, 2,2'- dihydroxy-4-methoxy benzophenone, 2,2 ', 4,4'-tetrahydroxy benzophenone, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxy-4'-methylbenzophenone, 2-hydroxy-4-methoxybenzophenone 5-sulfonate, 4-phenylbenzophenone, (2-ethylhexyl) -4'-phenylbenzophenone-2-carboxylate, 2-hydroxy-4- (n-octyloxy) benzophenone, 4-hydroxy-3-carboxybenzophenone; 3- (4'-methylbenzylidene) d, l-camphor, 3- (benzylidene) d, 1-camphor, benzalkonium methosulfate camphor; urocanic acid, ethyl urocanate; the family of sulfonic acid derivatives such as sulfonic acid 2-phenyl benzimidazole-5 and its salts; the family of triazine derivatives such as hydroxyphenyl triazine, (ethylhexyloxy) (hydroxyphenyl) (4-methoxyphenyl) triazine, 2,4,6-trianillin-β-carbo-2'-ethylhexyl-1 ' -oxy) -1,3,5-triazine, 4,4 - ((6 - (((1,1-dimethylethyl) amino) carbonyl) phenyl) amino) -1,3,5-triazine-2, Benzoic acid 4-diyl diimino) bis (2-ethylhexyl) ester, 2-phenyl-5-methylbenzoxazole, 2- (2'-hydroxy-5'-methylphenyl) benzotriazole, 2- (2-ethylhexyl) 5'-t-octyl phenyl) benzotriazole, 2- (2'-hydroxy-5'-methylphenyl) benzotriazole; dibenzazine; dianisoylmethane, 4-methoxy-4-tert-butylbenzoylmethane, 5- (3,3-dimethyl-2-norbornylidene) -3-pentan-2-one, the family of diphenylacrylate derivatives such as (2-ethyl) hexyl) 2-cyano-3,3-diphenyl-2-propenoate, ethyl-2-cyano-3,3-diphenyl-2-propenoate, the family of polysiloxanes such as benzylidene malonate siloxane.

 Among the inorganic solar filters, also called "inorganic screens", which can be associated with the glycolic extract of said unialgal biomass of small-sized multicellular macroalgae cells in the composition (C1), mention may be made of titanium oxides , zinc oxides, cerium oxide, zirconium oxide, iron oxides yellow, red or black, chromium oxides. These inorganic screens may or may not be micronized, have undergone or not surface treatments and may be presented in the form of aqueous or oily pre-dispersions.

 The following examples illustrate the invention without limiting it.

Samples tested to show the technical effect

 Extract A: glycolic extract (water-1, 3-propanediol mixture 60/40 mass) containing 4% by weight of a lyophilized biomass extract of the alga Ochlochaete hystrix, obtained by the process according to the invention

Biological effectiveness

 Choice of model and relevance:

 The model chosen to highlight the technical effect of our products is a model for the study of gene expression on human skin explants. So-called genomic and transcriptomic tests are widely used to highlight biological benefits.

Protocol: Explants of abdominal human skin approximately ten millimeters in diameter, taken from a Caucasian female donor about fifty years old, are prepared. They are kept alive in a specific BEM medium (Bio-Ec's ™ Explants Medium) at 37 ° C in a humid atmosphere containing 5% carbon dioxide.

 Extract A is then added, both in the culture medium, solubilized with 1% ethanol and topically, on three explants, vectorized in a hydroalcoholic gel containing 2.5% ethanol. . The explants so treated are left twenty-four hours.

 We operate in the same way on three other explants with the vector alone, that is to say without extract A. (control)

 At the end of this period, the total RNAs are extracted and their concentration and levels of purity and integrity are determined before being subjected to an amplification step.

 500ng of RNA are amplified which allows to obtain complementary RNA (cRNA), labeled with a fluorophore.

750 ng of these cRNAs are hybridized on HT12v4 human slides comprising synthetic DNA sequences corresponding to each human gene. The hybridization of the transcriptome with some of these DNA sequences is then revealed using a slide scanner and the data analyzed using a suitable software. Relative expression values of certain genes (Δ _βχρΓ .) _Are thus obtained in comparison with the controls.

 The results obtained are recorded in the following table:

Gene Full name (in English) (Δ _θ χρι-)

CREB1 cAMP responsive element binding protein 1 1, 36

CTNNB1 βΐ -cartenin 1, 31

FUT8 Fucosyl transferase 8 1, 41

MACF1 Microtube-actin crosslinking factor 1, 34

DST Dystonin 1, 32

MBNL2 Muscleblind-Like Splicing Regulator 2 1, 60

LIN7C lin-7 C-type, crumbs cell polarity complex component 1, 52

G3BP1 GTPase Activating Protein (SH3 Domain) Binding Protein 1 1, 32

DEK DEK (DEK Proto-Oncogene) 1, 47

GNA13 Guanine Nucleotide Protein Binding (G Protein), Alpha 13 1, 43

BNIP3L BCL2 / Adenovirus E1 B 19kDa Interacting Protein 3-Like 1, 66

CD164 cluster of differentiation 164 1, 60

COPS2 cOP9 signalosome subunit 2 1, 72 NOC3L (Nucleolar comples associated 3 homolog / FAD24) 1, 39

MTHFD2 Methylenetetrahydrofolate Dehydrogenase (NADP + Dependent) 2 1 .39

SACM1 SAC1 Suppressor Of Actin Mutations 1 -Like 1, 41

HECA Hdc Homolog, Cell Cycle Regulator 1, 33

KLF5 Kruppel-like Factor 5 1, 51

KFL3 Kruppel-like Factor 3 1, 27

ITGA6 lntegrin-a 6 1, 39

LAMB1 Laminin-β 1 1, 36

UGDH UDP-glucose 6-dehydrogenase 1, 40

THBS1 Thrombospondin 1 1, 38

LCE3D Late Cornified Envelope 3D 0.58

SPRR1 B Small proline-rich protein 1 B 0.59

SPRR2A Small proline-rich protein 2A 0.57

SPRR2B Small proline-rich protein 2B 0.54

SPRR2C Small proline-rich protein 2C 0.60

SPRR2G Small proline-rich protein 2G 0.72

CRCT1 Cysteine-rich C-terminal 1 0.64

DEFB4 Defensin- 4 0.50

HSPE1 Heat shock 10kDa protein 1 0.77

MKKS McKusick-Kaufman Syndrome 0.89

GSDMA Gasdermin A 0.70

MMP9 Matrix metalloproteinase-9 0.61

Out of two hundred and twenty-two genes modulated in response to extract A, a relative over-expression was observed for ninety-three of them (Δ _βχρΓ .≥ 1, 25) and a relative sub-expression per cent twenty-nine of them (Δ _βχρΓ <0.80). Only the most representative results are shown in the previous table.

These genes can be grouped into different biological processes and functions, as shown in the following table: Gene Biological Process Biological Action

 Inhibition of cell differentiation

CREB1, Regulation of and Promoting the Proliferation of CTNNB1 Epidermal Differentiation of Stem Cells by Activating the Wnt Pathway

 Regulation of the

 NOC3L Promotes cell proliferation

 epidermal differentiation

 Promotes maintenance stump condition

Regulation of the

 KLF5 cells and migration of epidermal differentiation

 kératinicytes

 LCE3D, SPRR Implication in differentiation

 Regulation of the

1 B, 2A, 2B, 2C, terminal and epidermal differentiation envelope formation

2G, CRCT1 horny

 Regulation of overexpressed during differentiation

DEFB4

 epidermal differentiation keratinocyte

 Strengthening links Implication in the cytoskeleton

MACF1, DST

 cells / cell matrix and adhesion junctions

Strengthening links

 THNS1, ITGA6 Role in cell / matrix cell / matrix adhesion

 Reinforcement of the matrix Role in the biosynthesis of

UGDH

 Extracellular dermal glycosaminoglycans

Reinforcement of the matrix Role in the degradation of the matrix

MMP-9

 extracellular dermal dermal

Extract A thus makes it possible to regulate three major skin processes:

 - Epidermal differentiation, which is limited to the benefit of cell proliferation and protection of stem cells;

 - The reinforcement of links matrix cells, in favor of a better cohesion between the cells and the matrices as well as between the cells themselves;

 - Reinforcement of the extracellular dermal matrix, which involves an increase in the essential components of its structure and cell signaling (CAG) and a limitation of its degradation.

Examples of cosmetic formulations containing extract A Dermo-purifying oil-in-water emulsion

Water qs 100%

Glycerin 3%

 Solagum ™ AX 0.3%

Montanov ™ 202 2%

 Lanol ™ 99 7%

 Ceiol ™ OE 3%

Lanol ™ P 0.25%

Sepiplus ™ 400 0.8%

Euxyl ™ PE9010 1%

 Sensiva ™ PA40 0.5%

Extract A 1%

Lactic acid at 20% qs pH = 5.5

Oil-in-water anti-sebum emulsion

Water qs 100%

 Montanov ™ 202 3%

 Montanov ™ 14 1, 5%

Pelemol ™ BB 2%

 Shea Butter 1, 5%

Phytosqualane 3%

 Jojoba oil 3%

 Triglyceride C8-C10 3%

 DUB ISIP 3%

 0.1% α-tocopherol

Solagum ™ Tara 0.6%

Extract A 2%

 Sorbic acid 0.3%

Soda 48% 0.07% Soothing serum

Sepimax ™ Zen 0.5%

 Water qs 100%

Butylene glycol 2%

Aquaxyl ™ 2%

 Extract A 1%

 Montanox 20 1%

 Phenoxyethanol & Ethylhexyl Glycerin 0.80%

SOLAGUM ™ AX: A mixture of acacia gum and xanthan gum used as an emulsifying agent;

 MONTANOV ™ 202 (INCI name: Arachidyl Alcohol & Behenyl Alcohol & Arachidyl Glucoside): Emulsifying agent;

LANOL ™ 99: Isononyl isononanoate;

 Cetiol ™ OE (INCI name: Dicaprylyl ether): Fatty phase;

LANOL ™ P: Glycol palmitate;

 SEPIPLUS ™ 400 (INCI name: Polyacrylate-13 & Polyisobutene & Polysorbate 20): Polymeric thickener;

EUXYL ™ PE9010 (INCI name: phenoxyethanol and ethylhexylglycerin): Preservative;

 SENSIVA ™ PA40 (INCI name: Phenethyl Alcohol (and) Ethylhexylglycerin) / Antimicrobial Agent.

 MONTANOV ™ 14 (INCI name: Myristyl Alcohol & Myristyl Glucoside): Emulsifying agent;

 PELEMOL ™ BB: Behenyl Behenate;

 SOLAGUM ™ Tara: Tara gum used as an emulsifying agent;

SEPIMAX ™ Zen (INCI name: polyacrylate crosspolymer-6): thickening, emulsifying and stabilizing agent

AQUAXYL ™ (INCI name: Xylitylglucoside and Anhydroxylitol and Xylitol): Moisturizing composition;

 MONTANOX ™ 20 (INCI name: Polysorbate 20): Oil-in-water emulsifying agent

Claims

1. Process for obtaining a unialgal biomass of small and endophytic macroalgae macroalgae cells of macroalgae hosts, characterized in that it comprises the following successive stages:

 A step A) of sampling the natural environment from a sample of said macroalgae hosts;

 A step B) of preparing a unialgal sample of cells of said small-sized and endophytic macroalgae of macroalgae hosts, by their separation from their said macroalgae hosts, from the sample taken in step A ) previous ;

 A step C) of culturing said unialgal sample of small and endophytic macroalgae macroalgae cells of macroalgae obtained in step B), in sea water with at least one nitrogen source, to obtain an aqueous suspension of said unialgal biomass of small-sized multicellular macroalgae cells;

 A step D) of harvesting said unialgal biomass of small-sized multicellular macroalgal cells from said aqueous suspension obtained at the end of step C).

2. Method as defined in claim 1, characterized in that said micro-cellular macroalgae of small size and endophytes of macroalgae hosts, are green algae from the class of Ulvophyceae, of the order Ulvales, family of Ulvellaceae , of the genus Ochlochaete and the species Ochlochaete hystrix.

3. Method as defined in any one of claims 1 or 2, characterized in that said macroalgae hosts are red algae from the class of Polypodiospsida, of the order Osmundales, family Osmundaceae, genus Osmundea and Osmundea hybrida species.

4. Method as defined in any one of claims 1 to 3, characterized in that it further comprises a step E) of preparing a powder of said unialgal biomass of small-sized multicellular macroalgae cells obtained at step D).

5. Method as defined in claim 4, characterized in that, during step E), said unialgal biomass of small multicellular macroalgae cells obtained in step D) is frozen, lyophilized and ground to obtain the desired powder.

6. Process for the preparation of a glycolic extract of a unialgal biomass of small-sized and endophytic macroalgae macroalgae cells of host macroalgae, characterized in that it comprises the following successive steps:

 A step F) during which the powder of said unialgal biomass of small-sized multicellular macroalgae cells obtained in step D) or in step E) of the process as defined in any one of claims 1 at 5, is dispersed with stirring in a water-glycol mixture at a rate of 0.2% by weight to 20% by mass of biomass per 100% by mass of dispersion;

 - A step G) during which the dispersion obtained in step F) above, is separated into its immiscible phases, to obtain the expected glycolic extract.

7. The process as defined in claim 6, wherein the glycol used is 1,3-propanediol.

8. Process as defined in claim 7, characterized in that during step E):

 The water-glycol mixture used is a water-1,3-propanediol mixture whose mass content in 1,3-propanediol (ratio of mass 1,3-propanediol to total mass 1,3-propanediol and water) is included. between 25% and 100%,

 The dispersion in said water-glycol mixture is carried out at a rate of 0.2% by weight to 10% by mass of biomass per 100% by mass of dispersion; and

 - The stirring is maintained for one to two hours, then if necessary or if desired,

 - The mass content of 1, 3-propanediol is adjusted between 40% and 60% by addition of water;

9. Glycolic extract of a unialgal biomass of small-sized and endophytic macromalangular macroalgae cells obtained by the method comprising the following successive steps:

 A step A) of sampling the natural environment from a sample of said host macroalgae;

A step B) of preparing a unialgal sample of cells of said small-sized and endophytic macroalgae of macroalgae hosts, by their separation from their said macroalgae hosts, from the sample taken in the previous step A;

 A step C) of culturing said unialgal sample of small and endophytic macroalgae macroalgae cells of macroalgae obtained in step B), in sea water with at least one nitrogen source, to obtain an aqueous suspension of said unialgal biomass of small-sized multicellular macroalgae cells;

 A step D) of harvesting said unialgal biomass of small-sized multicellular macroalgal cells from said aqueous suspension obtained at the end of step C).

 - Optionally step E) of preparing a powder of said unialgal biomass of small-sized multicellular macroalgae cells obtained in step D).

 A step F) during which the powder of said unialgal biomass of small-sized multicellular macroalgal cells obtained in step D) or in step E) is dispersed with stirring in a water-glycol mixture at a rate of from 0.2% by weight to 20% by mass of biomass per 100% by mass of dispersion;

 - A step G) during which the dispersion obtained in step F) above, is separated into its immiscible phases, to obtain the expected glycolic extract.

10. glycolic extract as defined in claim 9, wherein the glycol used is 1,3-propanediol.

1 1. Glycolic extract as defined in one of Claims 9 or 10, characterized in that the powder of said unialgal biomass of small-sized multicellular macroalgae cells used in step E) of the process for its preparation is a powder of said unialgal biomass of green algae cells from the class Ulvophyceae, the order of the Ulvales, the family of Ulvellaceae, the genus Ochlochaete and the species Ochlochaete hystrix.

12. glycolic extract as defined in claim 1 1, characterized in that during step F) of the process for its preparation:

 the water-glycol mixture used is a water-1,3-propanediol mixture whose mass content in 1,3-propanediol (ratio of mass 1,3-propanediol to total mass 1,3-propanediol and water) is included between 25% and 100%,

the dispersion in said water-glycol mixture is carried out at a rate of from 0.2% by mass to 10% by mass of biomass per 100% by mass of dispersion; and the stirring is maintained for one to two hours, then if necessary or if desired,

 the mass content of 1,3-propanediol is adjusted between 40% and 60%.

13. Use of the glycolic extract of a unialgal biomass of small-sized multicellular macroalgae cells as defined in any one of claims 9 to 12, as an anti-aging cosmetic active ingredient

14. The use as defined in claim 13, wherein the glycolic extract of a unialgal biomass of small multicellular macroalgae cells is the glycolic extract of a unialgal biomass of green algae cells from the class. Ulvophyceae, Ulvales, Ulvellaceae, Ochlochaete and Ochlochaete hystrix as previously defined.

15. Cosmetic composition (C1), for topical use comprising at least one cosmetically acceptable excipient and an effective amount of the glycolic extract of a unialgal biomass of small-sized multicellular macroalgae cells as defined in any one of the claims. 9 to 12.

16. The composition as defined in claim 15, wherein the glycolic extract of a unialgal biomass of small-sized multicellular macroalgae cells, the glycolic extract of a unialgal biomass of green algae cells of the class Ulvophyceae, Ulvales, Ulvellaceae, Ochlochaete and Ochlochaete hystrix as previously defined.