WO2018004253A3 - 표적 단백질 검출을 위한 압타머-면역세포 바이오프로브 - Google Patents

표적 단백질 검출을 위한 압타머-면역세포 바이오프로브 Download PDF

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WO2018004253A3
WO2018004253A3 PCT/KR2017/006834 KR2017006834W WO2018004253A3 WO 2018004253 A3 WO2018004253 A3 WO 2018004253A3 KR 2017006834 W KR2017006834 W KR 2017006834W WO 2018004253 A3 WO2018004253 A3 WO 2018004253A3
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bioprobe
aptamer
target protein
immune cell
protein
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PCT/KR2017/006834
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French (fr)
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WO2018004253A2 (ko
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최종훈
황장선
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중앙대학교 산학협력단
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Publication of WO2018004253A2 publication Critical patent/WO2018004253A2/ko
Publication of WO2018004253A3 publication Critical patent/WO2018004253A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4737—C-reactive protein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/495—Transforming growth factor [TGF]
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 표적 단백질에 특이적으로 결합하는 압타머(aptamer), 및 상기 압타머와 링커(linker)를 통하여 연결된 면역세포를 포함하는 표적 단백질 검출용 바이오프로브(bioprobe)에 관한 것으로서, 단백질을 표적화하는 압타머가 살아있는 면역세포와 결합되어 제조된 실시간으로 체내외 단백질을 검출할 수 있는 바이오프로브를 제공한다. 또한, 상기 바이오프로브를 포함하는 단백질 검출용 진단 시약을 제공한다.
PCT/KR2017/006834 2016-07-01 2017-06-28 표적 단백질 검출을 위한 압타머-면역세포 바이오프로브 WO2018004253A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020160083261A KR101928620B1 (ko) 2016-07-01 2016-07-01 표적 단백질 검출을 위한 압타머-면역세포 바이오프로브
KR10-2016-0083261 2016-07-01

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WO2018004253A2 WO2018004253A2 (ko) 2018-01-04
WO2018004253A3 true WO2018004253A3 (ko) 2018-02-22

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PCT/KR2017/006834 WO2018004253A2 (ko) 2016-07-01 2017-06-28 표적 단백질 검출을 위한 압타머-면역세포 바이오프로브

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WO (1) WO2018004253A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108866063A (zh) * 2018-06-28 2018-11-23 中国水产科学研究院珠江水产研究所 一种peg修饰的核酸适配体及其制备方法和应用
KR102200143B1 (ko) 2019-01-31 2021-01-08 한국기술교육대학교 산학협력단 표적단백질 검출용 바이오프로브, 이의 제조 방법, 이를 갖는 분석장치 및 그 방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060014172A1 (en) * 2004-05-03 2006-01-19 Nanosphere, Inc. Aptamer-nanoparticle conjugates and method of use for target analyte detection
KR101088885B1 (ko) * 2008-12-23 2011-12-07 연세대학교 산학협력단 바이오프로브, 그 제조방법, 상기를 사용한 분석 장치 및 분석 방법
US20110318846A1 (en) * 2010-06-25 2011-12-29 National Cheng-Kung University Aptamer and detection method for C-reactive protein
US20130040837A1 (en) * 2009-12-01 2013-02-14 Brigham And Women's Hospital, Inc. Aptamer cell compositions
US20140017170A1 (en) * 2008-11-24 2014-01-16 Massachusetts Institute Of Technology Methods and compositions for localized agent delivery

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060014172A1 (en) * 2004-05-03 2006-01-19 Nanosphere, Inc. Aptamer-nanoparticle conjugates and method of use for target analyte detection
US20140017170A1 (en) * 2008-11-24 2014-01-16 Massachusetts Institute Of Technology Methods and compositions for localized agent delivery
KR101088885B1 (ko) * 2008-12-23 2011-12-07 연세대학교 산학협력단 바이오프로브, 그 제조방법, 상기를 사용한 분석 장치 및 분석 방법
US20130040837A1 (en) * 2009-12-01 2013-02-14 Brigham And Women's Hospital, Inc. Aptamer cell compositions
US20110318846A1 (en) * 2010-06-25 2011-12-29 National Cheng-Kung University Aptamer and detection method for C-reactive protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HWANG, JANGSUN ET AL.: "Aptamer-conjugated Live Human Immune Cell Based Biosensors for the Accurate Detection of C-reactive Protein", SCIENTIFIC REPORTS, vol. 6, 6 October 2016 (2016-10-06), pages 1 - 9, XP055465971 *

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KR20180004342A (ko) 2018-01-11
KR101928620B1 (ko) 2018-12-12

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