WO2017214839A1 - Kit de réactifs multifonctionnels - Google Patents

Kit de réactifs multifonctionnels Download PDF

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Publication number
WO2017214839A1
WO2017214839A1 PCT/CN2016/085644 CN2016085644W WO2017214839A1 WO 2017214839 A1 WO2017214839 A1 WO 2017214839A1 CN 2016085644 W CN2016085644 W CN 2016085644W WO 2017214839 A1 WO2017214839 A1 WO 2017214839A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
kit
kit according
multifunctional
control unit
Prior art date
Application number
PCT/CN2016/085644
Other languages
English (en)
Chinese (zh)
Inventor
彭鹏
Original Assignee
彭鹏
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 彭鹏 filed Critical 彭鹏
Priority to PCT/CN2016/085644 priority Critical patent/WO2017214839A1/fr
Publication of WO2017214839A1 publication Critical patent/WO2017214839A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16Z—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR
    • G16Z99/00—Subject matter not provided for in other main groups of this subclass

Definitions

  • the present invention relates to the field of biotechnology, and in particular to a multifunctional kit.
  • kits In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role.
  • the kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.
  • the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated.
  • the general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water.
  • the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples.
  • kits As the development of biology and the interdisciplinary processes of biology and other closely related disciplines increase, the variety of kits continues to increase. For the manufacturers of kits, especially for large manufacturers that produce a wide variety of widely used kits, the management of the kits produced has always been a very challenging task. At present, most manufacturers use the management method to record the relevant information of the kit in the paper document, and find the relevant documents to check if necessary. This manual recording and comparison management mode results in inefficient and high error rates for kit management, and the additional expenditure for "correction" generated each year amounts to millions or even tens of millions.
  • the present invention provides a multifunctional kit.
  • the technical solution adopted by the present invention is: a multifunctional kit comprising a kit body and a lid, the kit body is provided with a sounding device and a control unit, a small battery, a display device and a close range Wireless communication NFC tag.
  • the sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected; the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device and the temperature control probe And the control unit is electrically connected; the short-range wireless communication NFC tag is composed of a data storage module and a data transmission module, the output end of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the NFC chip to Intelligent management of the use of the staff
  • the inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further arranged between the two.
  • the lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.
  • the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.
  • the kit body has a two-layer structure, and the control unit, the sounding device and the small storage battery are disposed between the inner layer and the outer layer of the reagent cartridge body.
  • the number of the reagent wells is six.
  • the display device is a digital display screen.
  • the sounding device is a buzzer, and the number is two.
  • control unit is a single chip microcomputer.
  • the small battery is a lithium ion battery.
  • the inductor is connected to the bottom of the pipette tip collecting hole by a spring.
  • the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the sensor.
  • the arrow indicator is made of LED, connected to the sensor, and controlled by the sensor.
  • the smart terminal used by the staff is set on the mobile phone and displays related information of the kit through the mobile phone interface.
  • the present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device.
  • the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample.
  • the experiment failed; the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for the production enterprise Reduced management costs.
  • 2 is a structural diagram of the reagent well.
  • FIG. 3 is a schematic structural view of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.
  • a multifunctional kit includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 , a sounding device 40, and a control unit (not shown). ), a small battery (not shown), a display device 80, and a short-range wireless communication NFC tag 90.
  • the sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected.
  • the outer wall of the reagent hole is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
  • the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is further disposed therebetween, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36.
  • the sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32.
  • the rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated.
  • the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50.
  • the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32.
  • the color of the disk 36 is rotated and the arrow indicator light 34 is illuminated.
  • the color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36.
  • the eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively.
  • control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.
  • the short-range wireless communication NFC tag 90 mainly includes a data storage module 901, a data transmission module 902, and an NFC chip 903.
  • the output of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal (usually the mobile phone 910) used by the user through the NFC chip.
  • the present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once it exceeds the setting range, The sound device immediately alarms the experimenter to reduce the probability of the experiment failure; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.
  • the present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed; the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for the production enterprise Reduced management costs.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Temperature Or Quantity Of Heat (AREA)

Abstract

Kit de réactifs multifonctionnels. En fournissant un dispositif générateur de son (40), une unité de commande et une batterie de stockage au niveau d'un corps principal (10) du kit de réactifs, et en fournissant un dispositif de réfrigération (381) et une sonde de régulation de température (382) au niveau d'une paroi externe d'un évidement de réactifs, la présente invention fournit un environnement de pré-refroidissement désiré (2-8 °C) pour un réactif, et permet une détection de température au moyen de la sonde de commande de température (382), et l'affichage de la température sur un dispositif d'affichage (80) pour le personnel de laboratoire. Lorsque la température dépasse une plage préréglée, le dispositif générateur de son (40) donne immédiatement une alarme pour avertir le personnel de laboratoire, réduisant ainsi la probabilité de défaillances d'expérience. En même temps, en utilisant une plaque tournante de réactifs (36) pour entraîner en rotation des évidements de réactifs (38), la présente invention fournit un rappel de chargement au personnel de laboratoire pour empêcher des défaillances d'expérience provoquées par une erreur de chargement. En outre, en employant une transmission de données de bout en bout sans contact entre un terminal de gestion intelligent et le kit de réactifs dans une plage de cinq centimètres, la présente invention résout de nombreux problèmes de procédés de gestion de fabrication de kits de réactifs classiques, améliorant ainsi l'efficacité et la précision de gestion, et réduisant le coût de gestion du fabricant.
PCT/CN2016/085644 2016-06-14 2016-06-14 Kit de réactifs multifonctionnels WO2017214839A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/085644 WO2017214839A1 (fr) 2016-06-14 2016-06-14 Kit de réactifs multifonctionnels

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/085644 WO2017214839A1 (fr) 2016-06-14 2016-06-14 Kit de réactifs multifonctionnels

Publications (1)

Publication Number Publication Date
WO2017214839A1 true WO2017214839A1 (fr) 2017-12-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2016/085644 WO2017214839A1 (fr) 2016-06-14 2016-06-14 Kit de réactifs multifonctionnels

Country Status (1)

Country Link
WO (1) WO2017214839A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060190185A1 (en) * 1997-08-11 2006-08-24 Ventana Medical Systems, Inc. Memory management method and apparatus for automated biological reaction system
CN203259385U (zh) * 2013-03-12 2013-10-30 深圳华大基因研究院 生物样品分配防错设备及系统
CN203465107U (zh) * 2013-08-28 2014-03-05 中国水产科学研究院南海水产研究所 用于元素分析仪中的可分式进样盘机构
CN204241706U (zh) * 2014-12-06 2015-04-01 周振 红外感应微孔板指示仪
CN204779612U (zh) * 2015-06-09 2015-11-18 盐城卫生职业技术学院 一种细胞培养板
CN205003156U (zh) * 2015-10-20 2016-01-27 苏州荣磐医疗科技有限公司 一种运用近距离无线通讯传输的快速血液测试装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060190185A1 (en) * 1997-08-11 2006-08-24 Ventana Medical Systems, Inc. Memory management method and apparatus for automated biological reaction system
CN203259385U (zh) * 2013-03-12 2013-10-30 深圳华大基因研究院 生物样品分配防错设备及系统
CN203465107U (zh) * 2013-08-28 2014-03-05 中国水产科学研究院南海水产研究所 用于元素分析仪中的可分式进样盘机构
CN204241706U (zh) * 2014-12-06 2015-04-01 周振 红外感应微孔板指示仪
CN204779612U (zh) * 2015-06-09 2015-11-18 盐城卫生职业技术学院 一种细胞培养板
CN205003156U (zh) * 2015-10-20 2016-01-27 苏州荣磐医疗科技有限公司 一种运用近距离无线通讯传输的快速血液测试装置

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