WO2017209341A1 - Corps de structure à maillage tridimensionnelle et turbine ayant un corps de structure à maillage tridimensionnelle - Google Patents

Corps de structure à maillage tridimensionnelle et turbine ayant un corps de structure à maillage tridimensionnelle Download PDF

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Publication number
WO2017209341A1
WO2017209341A1 PCT/KR2016/006866 KR2016006866W WO2017209341A1 WO 2017209341 A1 WO2017209341 A1 WO 2017209341A1 KR 2016006866 W KR2016006866 W KR 2016006866W WO 2017209341 A1 WO2017209341 A1 WO 2017209341A1
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Prior art keywords
mesh structure
dimensional mesh
group
plate
cells
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PCT/KR2016/006866
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English (en)
Korean (ko)
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김중배
홍성길
김한솔
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고려대학교 산학협력단
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Priority claimed from KR1020160066446A external-priority patent/KR101869350B1/ko
Priority claimed from KR1020160066338A external-priority patent/KR101830198B1/ko
Application filed by 고려대학교 산학협력단 filed Critical 고려대학교 산학협력단
Publication of WO2017209341A1 publication Critical patent/WO2017209341A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis

Definitions

  • the present invention relates to an impeller having a three-dimensional mesh structure and a three-dimensional mesh structure in which materials such as organic catalysts, inorganic catalysts and biomolecules are highly integrated.
  • Impeller is a device that regulates the flow and mixing of the fluid by rotating the fluid using external power. Impellers have been developed in various ways according to their purpose and use, and there are representative propeller type, turbine type, and screw type impellers.
  • impellers For practical applications, a number of impellers have been developed for various applications, such as industrial applications requiring precise control of fluid flow to households for simple mixing. In general, the impeller is mainly used for the uniform mixing of the fluid in the reactor or stirred tank.
  • the impeller uniformly mixes the reactants in the fluid and at the same time, useful materials such as catalysts attached to the impeller surface play an important role in improving the overall reaction efficiency.
  • useful materials such as catalysts attached to the impeller can be easily reused, thereby simplifying the process.
  • the present invention provides an impeller having a three-dimensional mesh structure and a three-dimensional mesh structure that can promote reactivity by highly integrating materials such as organic catalysts, inorganic catalysts, and biomolecules in a mesh structure having a three-dimensional shape.
  • the purpose is to.
  • a support having a three-dimensional mesh structure; And a fiber aggregate embedded in the support and capable of bonding with the first material. It provides a three-dimensional mesh structure comprising a.
  • the support may be formed by combining at least two plate-like structure having a mesh structure.
  • the plate-like structure is provided with a plurality of stacked, the fiber assembly may be embedded between the adjacent plate-like structure of the plurality of plate-like structure.
  • a protrusion is formed at one side edge of any of the first plate-like structures of the plate-like structure, the insertion groove into which the protrusion is inserted is formed at the edge of one side of the second plate-like structure adjacent to the first plate-like structure.
  • an insertion groove is formed in each of the at least two plate-like structures, and after the stacking of the at least two plate-like structures, a coupling rod may be fitted into the insertion groove.
  • both sides of the stacked plate-shaped structures may be coupled to each other by a coupling member in the form of a clip.
  • the first material may include an organic catalyst, an inorganic catalyst, and a biomolecule
  • the fiber aggregate may include a functional group bonded to the first material, and at least one of the organic catalyst, an inorganic catalyst, and a biomolecule may be used. It may be bound to the fiber aggregates by adsorption, ionic bonding, covalent bonding, crosslinking or adhesive materials.
  • the functional group is a carboxyl group, amine group, imine group, epoxy group, hydroxyl group, aldehyde group, carbonyl group, ester group, methoxy group, ethoxy group, peroxy group, ether group, acetal group, sulfide group, phosphate group and iodine group It may include at least one of.
  • porous particles may be adsorbed, ionic, covalent, crosslinked or adhesive. It can be coupled to the fiber lumps by.
  • the fibers are polyaniline, polypyrrole, polythiophene, acrylonitrile-butadiene-styrene, polylactic acid, polyvinyl alcohol, polyacrylonitrile, polyester, polyethylene, polyethyleneimine, polypropylene oxide, polyvinyl Lidane fluoride, polyurethane, polyvinyl chloride, polystyrene, polycaprolactam, polylactic-co-glycolic acid, polyglycolic acid, polycaprolactone, polyethylene terephthalate, polymethylmethacrylate, polydimethylsiloxane, teflon , Collagen, polystyrene-co-maleic anhydride, nylon, cellulose, chitosan, and may include a polymer fiber comprising at least one selected from silicon and a polymer fiber modified to form a functional group.
  • the polymer fibers may be aniline (pyrrole), lactic acid (lactic acid), vinyl alcohol (vinyl alcohol), acrylonitrile (acrylonitrile), ethylene (ethylene), ethylene imine (ethyleneimine), propylene oxide (propylene oxide), urethane, vinyl chloride, styrene, caprolactam, caprolactone, aprolactone, ethylene terephthalate, methyl methacrylate
  • a first monomer comprising at least one selected from dimethylsiloxane, teflon, collagen, nylon, cellulose, cellulose, chitosan and silicon; ; And aminobenzoic acid (1-aminobenzoic acid), 2-aminobenzoic acid, 3-aminobenzoic acid, 3-aminobenzoic acid, 1-phenylenediamine, 2-phenyl 2-phenylenediamine, 3-phenylenediamine, pyrrole-1-carbaldehyde, pyrrole-2-carbaldehyde
  • the support may be acrylonitrile-butadiene-styrene, polyaniline, polypyrrole, polythiophene, polylactic acid, polyvinyl alcohol, polycaprolactam, polycaprolactone, polylactic-co-glycolic acid, polyacrylo Nitrile, polyester, polyethylene, polyethyleneimine, polypropylene oxide, polyurethane, polyglycolic acid, polyethylene terephthalate, polymethylmethacrylate, polystyrene, polydimethylsiloxane, polystyrene-co-maleic anhydride, teflon, collagen, nylon , Cellulose, chitosan, glass, gold, silver, aluminum, iron, copper and silicon, the organic catalyst comprising carbonic anhydrase, glycosylating enzyme, trypsin, chymotrypsin, subtilisin, papain , Thermolysine, lipase, peroxidase, acylase, lact
  • the cells include stem It may include one or more selected from cells, immune cells, epithelial cells, muscle cells, neurons, hepatocytes, lung cells, cardiovascular cells, pancreatic cells, heart cells, bone cells and cancer cells.
  • the coupling portion is coupled to the rotation axis in the center; And an impeller coupled to the coupling part and including one or more three-dimensional mesh structures according to the above-described features.
  • the three-dimensional mesh structure may be a single body.
  • the three-dimensional mesh structure may be provided with a connection portion to be detachable to the coupling portion.
  • different materials may be fixed to each of the one or more three-dimensional mesh structures.
  • the organic catalyst, inorganic catalyst and biomolecules are highly integrated on the surface of the mesh structure of the three-dimensional shape, and reacts with the fluid, compared to the prior art There is an advantage that the reaction can proceed at a high rate.
  • the fiber aggregates are embedded in each of the plurality of internal spaces formed between the plurality of plate-like structures, thereby improving the immobilization amount of materials such as organic catalysts, inorganic catalysts and biomolecules in the fiber aggregates.
  • the three-dimensional mesh structure by making the impeller detachable from the coupling portion is easy to replace as necessary, there is an advantage that can be easily reused after the reaction is finished.
  • a chain reaction may occur by supporting different materials on each of the three-dimensional mesh structures provided with at least one, and various applications may be possible.
  • various biomolecules such as antibodies capable of selective binding
  • the mesh structure is immobilized on the mesh structure to specifically bind microorganisms or cells, and then the three-dimensional mesh structure is desorbed to culture and activate the bound microorganisms and cells, thereby diagnosing the immune cells. It is expected that new applications will be created in various medical fields such as treatment and cell chip development.
  • FIG. 1 is a perspective view schematically showing a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG. 2 is an exploded perspective view of a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG 3 is a cross-sectional view of a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG. 4 is a perspective view showing another example of a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG. 5 is an exploded perspective view showing another example of a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG. 6 is a cross-sectional view showing another example of a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG. 7A and 7B are views illustrating a method of joining a plate-like structure in a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG 8 to 11 is a perspective view showing an impeller having a three-dimensional mesh structure according to an embodiment of the present invention.
  • FIG. 13 illustrates experimental data obtained by immobilizing GOx to a 3D mesh structure according to an exemplary embodiment of the present invention.
  • 3D mesh structure 120 may include a support 121 and the fiber assembly 150, as shown in Figures 1 and 2.
  • a plurality of porous holes 128 through which the reaction solution may pass may be formed on the outer circumferential surface of the support 121, and an inner space 129 may be formed on the inner circumferential surface of the support 121.
  • the support 121 may include a pair of plate-like structures 125a and 125b having a mesh structure. That is, the support 121 may be formed by the combination of a pair of plate-shaped structure (125a, 125b).
  • the plate-like structure 125 may include an opening opened to one side, and a plurality of porous holes 128 may be formed on the outer circumferential surface.
  • an inner space 129 is formed therein (see FIG. 3). That is, the opening 122a of the first plate-like structure 125a and the opening 122b of the second plate-like structure 125b are combined to form an inner space 129.
  • the coupling of the first and second plate-like structure may be coupled to each other by fitting coupling.
  • an insertion groove 123 may be formed at one edge of the first plate-like structure 125a, and at one edge of the second plate-like structure 125b facing one surface of the first plate-like structure 125a.
  • the protrusion 124 may be formed.
  • the protrusion 124 may be inserted into and inserted into the insertion groove 123 so that assembly of the support 121 may be completed.
  • an inner space 129 may be formed inside the support 121.
  • the fiber assembly 150 may be disposed in the inner space 129.
  • the fiber assembly 150 is disposed between the first and second plate-like structures 125a and 125b, and the first and second plate-shaped structures 125a and 125b.
  • the fiber assembly 150 may be embedded therein, thereby completing the three-dimensional mesh structure 120.
  • the support 121 is shown and described as being formed by a combination of a pair of plate-like structure, the present invention is not limited thereto. That is, the support may be formed by combining a plurality of plate-like structures.
  • the three-dimensional mesh structure 120 ′ may include a support 121 ′ and a fiber assembly 150 ′, as shown in FIGS. 4 and 5. have.
  • the support 121 ′ may include a plurality of plate-shaped structures 125 ′ having a mesh structure. That is, the support 121 'may be formed by combining a plurality of plate-shaped structures 125'.
  • the plate-like structure 125 ′ may include an opening opened to one side, and a plurality of porous holes 128 ′ may be formed on an outer circumferential surface thereof.
  • a plurality of plate-shaped structures 125a ', 125b', 125c ', and 125d' having such a configuration are coupled to each other, a plurality of internal spaces 129a ', 129b', and 129c 'are formed therein.
  • the support 121 ' will be formed by four plate-like structures.
  • the plurality of plate-shaped structures 125a ', 125b', 125c ', and 125d' may be stacked in the horizontal direction to form the support 121 '.
  • the plurality of plate structures 125a are formed such that a plurality of inner spaces 129a ', 129b', and 129c 'are formed by openings formed in the plurality of plate structures 125a', 125b ', 125c', and 125d ', respectively. ', 125b', 125c ', 125d') are stacked.
  • the opening of the second plate-like structure 125b 'and the opening of the third plate-like structure 125c' face each other to form a second inner space 129b ', and one surface of the second plate-like structure 125b'.
  • the first inner space 129a ' is formed by engaging with the opening of the first plate-like structure 125'a, and one surface of the third plate-like structure 125c' is coupled with the opening of the fourth plate-like structure 125d '.
  • the third internal space 129c ' may be formed.
  • fiber aggregates 150 ' may be embedded in the first to third internal spaces 129a', 129b ', and 129c', respectively.
  • the first to fourth plate-like structures 125'a and 125d ' may be coupled by fitting coupling.
  • the second and third plate-like structures 125b 'and 125c' have protrusions 124 'and insertion grooves 123' formed at both edges thereof. That is, protrusions 124 'are formed at one edges of the second and third plate-like structures 125b' and 125c ', and insertion grooves 123' are formed at the other edges.
  • first and fourth plate-like structures 125'a and 125d ' have insertion grooves 123' or protrusions 124 'formed on one surface thereof. That is, an insertion groove 123 'is formed in one surface of the first plate-shaped structure 125a' disposed at the outermost side, and a protrusion 124 'is formed in one surface of the fourth plate-shaped structure 125d'.
  • the plurality of plate structures 125a ', 125b', 125c ', and 125d' are assembled, the plurality of plate structures 125a ', respectively, are inserted after the fiber assemblies 150' are inserted between the adjacent plate structures.
  • 125b ', 125c', and 125d ' are assembled to complete the three-dimensional mesh structure 120'.
  • the three-dimensional mesh structure 120, 120 ′ having such a structure has a fiber aggregate embedded in each of a plurality of internal spaces formed between the plurality of plate-like structures, thereby allowing the fiber aggregate to contain materials such as organic catalysts, inorganic catalysts, and biomolecules.
  • the amount of immobilization can be improved.
  • the 3D mesh structure 120 and 120 ' is illustrated and described as being provided in plural numbers, the present invention is not limited thereto, and the 3D mesh structure 120 is not limited thereto.
  • the supports 121 and 121 ′ may be formed in a rectangular parallelepiped shape, and the inside and the outside of the three-dimensional mesh structure 120 and 120 ′ are formed in a mesh shape according to the size of the reaction apparatus and the shape of the reactant.
  • the overall size of the 3D mesh structures 120 and 120 ' may be adjusted.
  • the size of the porous holes (128, 128 ') of the support (121, 121') can also be adjusted according to the size of the reactor and the shape of the reactants, the position of the porous holes (128, 128 ') also reactant It can be changed according to the shape.
  • the present invention is not limited thereto. That is, since the fiber aggregate has a thin thickness, it may be pressed and inserted between the plate-like structures even when there is no internal space in the support.
  • the at least two or more plate-like structure is shown and described as being fitted by the insertion groove and the protrusion formed on the edge, but the present invention is not limited thereto.
  • insertion plates are formed in the plate-shaped structures, respectively, and the coupling rods 161 may be fitted into the insertion grooves after the plate-shaped structures are stacked.
  • the coupling members 162 in the form of clips may be coupled to both sides of the stacked plate-like structures as shown in FIG. 7B.
  • the fiber assemblies 150 and 150 ′ according to FIGS. 1 to 6 may have organic catalysts, inorganic catalysts, and biomolecules fixed on the surfaces thereof to be embedded in the internal spaces of the supports 121 and 121 ′.
  • the organic catalyst, the inorganic catalyst and the biomolecules may be bonded to the fiber assemblies 150 and 150 '.
  • the organic catalyst, the inorganic catalyst and the biomolecule may be fixed to the surface of the fiber assembly 150, 150 'by adsorption, ionic bonding, covalent bonding, crosslinking or adhesive material.
  • the organic catalyst, the inorganic catalyst, and the biomolecule may be fixed to the surface of the fiber assembly 150 or 150 'by using one of covalent bonds, ionic bonds, and crosslinks. If not fixed through the fiber aggregate (150, 150 ') using one of catechol-based adhesives such as polydopamine, polynorepinephrine, and physical simple adsorption It can be fixed to the surface of the.
  • the fiber aggregates 150 and 150 ' may be formed of porous particles, carbon tubes, polymer tubes, wires, pillars, graphene, fullerenes, polydopamine, poly by adsorption, ionic bonding, covalent bonding, crosslinking, or adhesive materials. It is noted that at least one or more of norepinephrine and spherical particles may additionally be attached.
  • materials such as the organic catalyst, the inorganic catalyst, and the biomolecule may be fixed to the fiber assemblies 150 and 150 '.
  • the organic catalyst, the inorganic catalyst and the biomolecules may be directly or indirectly bonded to the fiber aggregates 150 and 150 '.
  • the organic catalyst, the inorganic catalyst and the biomolecule may be directly fixed to the fiber aggregate 150, 150 'by adsorption, ionic bond, covalent bond, crosslinking and adhesive material, preferably the organic Catalysts, inorganic catalysts and biomolecules can be directly bonded through covalent bonds with the functional groups of the fiber aggregate.
  • the functional group of the polymer fiber and the biomolecule are specifically bound by heterogeneous biomolecules, so that the functional group and the biomolecule are indirectly bound through heterogeneous biomolecules serving as a linker, thereby forming the bio-molecule on the polymer fiber.
  • Molecules may be immobilized, more specifically, the specific binding is antibody-antigen, protein A-antibody, protein G-antibody, nucleic acid-nucleic acid hybrid, aptamer-biomolecule, avidin-biotin ), Through the specific binding of streptavidin-biotin, lectin-carbohydrate, lectin-glycoprotein, etc., between the functional group and the biomolecule.
  • the molecule may act as a linker so that the functional group of the polymer fiber and the biomolecule may be indirectly bonded.
  • polymer fibers are used as the fiber aggregates 150 and 150 ', and the polymer fibers including functional groups include polyaniline, polypyrrole, polythiophene, acrylonitrile-butadiene-styrene, polylactic acid, and polyvinyl alcohol.
  • Polyacrylonitrile polyester, polyethylene, polyethyleneimine, polypropylene oxide, polyvinylidene fluoride, polyurethane, polyvinyl chloride, polystyrene, polycaprolactam, polylactic-co-glycolic acid, polyglycolic acid, Polymers such as polycaprolactone, polyethylene terephthalate, polymethylmethacrylate, polydimethylsiloxane, Teflon, collagen, polystyrene-co-maleic anhydride, nylon, cellulose, chitosan and silicone What formed functional groups in a fiber can be used.
  • the fiber aggregate may produce a polymer fiber having a functional group while polymerizing the polymer fiber polymerization solution to form a polymer or copolymer.
  • the polymer fibers with functional groups are copolymers such as aniline, pyrrole, lactic acid, vinyl alcohol, acrylonitrile, ethylene, ethyleneimine ), Propylene oxide, urethane, vinyl chloride, styrene, caprolactam, caprolactone, aprolactone, ethylene terephthalate, methyl methacrylate (Methyl methacrylate), dimethylsiloxane (dimethysiloxane), Teflon (teflon), collagen (collagen), nylon (nylon), cellulose (cellulose), chitosan (silic acid) and silicon (silicon) A first monomer; And aminobenzoic acid (1-aminobenzoic acid), 2-aminobenzoic acid, 3-aminobenzoic acid, 3-aminobenzobenz
  • the fiber assembly 150, 150 ' may not include a functional group. That is, unlike a copolymer including a functional group, in the case of a polymer fiber grown with a polymer containing no functional group, a separate reforming reaction may be performed to form functional groups in the polymer fiber.
  • the organic catalyst is carbonic anhydrase, glycosylating enzyme, trypsin, chymotrypsin, subtilisin, papain, thermolysine, lipase, peroxidase, acylase, lactonase, protease, tyrosinase, laccase At least one of enzymes including at least one of cellulase, xylanase, organophosphohydrolase, cholinesterase, formic acid dehydrogenase, aldehyde dehydrogenase, alcohol dehydrogenase, glucose dehydrogenase, and glucose isomerization enzyme. And
  • the inorganic catalyst is platinum, platinum, rhodium, palladium, lead, iridium, rubidium, iron, nickel, zinc, cobalt, copper, manganese, titanium, ruthenium, silver, molybdenum, tungsten, aluminum, iron, antimony, tin, It may include at least one of bismuth, barium, osmium, nitric oxide, copper oxide, manganese oxide, titanium oxide, vananium oxide, and zinc oxide.
  • the biomolecule may include at least one of enzyme, albumin, insulin, collagen, antibody, antigen, protein A, protein G, avidin, streptavidin, biotin, nucleic acid, peptide, lectin, carbohydrate.
  • the support bodies 121 and 121 'of the three-dimensional mesh structures 120 and 120' may be used as long as they can grow polymer fibers containing functional groups on the surface thereof.
  • the support is made of acryl. Nitrile-butadiene-styrene, polyaniline, polypyrrole, polythiophene, polylactic acid, polyvinyl alcohol, polycaprolactam, polycaprolactone, polylactic-co-glycolic acid, polyacrylonitrile, polyester, polyethylene, Polyethyleneimine, polypropylene oxide, polyurethane, polyglycolic acid, polyethylene terephthalate, polymethyl methacrylate, polystyrene, polydimethylsiloxane, polystyrene-co-maleic anhydride, teflon, collagen, nylon, cellulose, chitosan, At least one of glass, gold, silver, aluminum, iron, copper and silicon can be used, but the invention is not so limited.
  • the three-dimensional mesh structure 120 having such a structure is highly integrated with the catalyst material or the biomolecule on the surface and inside of the support having the three-dimensional mesh structure, the overall reaction area can be widened, and the catalyst and the biomolecule are highly integrated. The reaction yield can be improved by this.
  • the organic catalyst, the inorganic catalyst and the biomolecules are highly integrated on the surface and the inside of the three-dimensional mesh structure and react with the fluid, there is an advantage that the reaction may proceed at a high speed as compared with the prior art.
  • the three-dimensional mesh structure (120, 120 ') having the configuration as described above can be various applications depending on the first material to be bonded.
  • the antibody may be attached to the fiber aggregates 150 and 150 'provided in the supports 121 and 121' to selectively bind microorganisms and cells, and to culture and activate them.
  • the microorganism is Bacillus subtilis, Bacillus licheniformis, Bacillus polyfermenticus, Bacillus mesentericus, Saccharomyces cerevises cerevisiae, Clostridium butyricum, Streptococcus faecalis, Streptococcus faecium, Micrococcus caseolyticus, Staphylococcus aureus auretaloclocus ), Lactobacillus casei, Lactobacillus plantarum, Leuconostoc Mesenteroides, Saccharomyces cerevisiae, Debariomyses nicotiana nicotianae), Acinetobacter calcoaceticus, alkali Alcaligenesodorans, Aromatoleum aromaticum, Geobacter metallireducens, Dechloromonas aromatic, Arthrobacter sp. And Alkanivolas bors It may include one or more selected from the cumulus (Al, Bacill
  • the cells may include one or more selected from stem cells, immune cells, epithelial cells, muscle cells, nerve cells, hepatocytes, lung cells, cardiovascular cells, pancreas cells, heart cells, bone cells and cancer cells.
  • the three-dimensional mesh structures 120 and 120 ′ having the above configuration may be provided in plural to form impellers 100 and 100 ′.
  • Impellers 100, 100 ′ having three-dimensional mesh structures 120 and 120 ′ may include coupling portions 110 and 110 ′ and three-dimensional mesh structures 120 and 120 ′. Can be.
  • the coupling parts 110 and 110 ′ are for rotating one or more three-dimensional mesh structures 120 and 120 ′ in one direction and have a cube shape.
  • rotating shafts 130 and 130 'for rotating the 3D mesh structure 120 and 120' in one direction may be coupled to the upper portions of the coupling parts 110 and 110 ', as shown in FIG.
  • coupling holes 111 and 111 ′ may be formed to couple the 3D mesh structures 120 and 120 ′.
  • the shape of the coupling parts 110 and 110 ′ is described and illustrated as being formed in a cube shape, but is not limited thereto. Specifically, it may be formed in a hexahedral shape or a spherical shape, and may be formed in any shape as long as it is combined with the one or more three-dimensional mesh structures to rotate in one direction.
  • One or more three-dimensional mesh structures 120 and 120 ' may be coupled to side surfaces of the coupling portions 110 and 110', and more specifically, formed on side surfaces of the coupling portions 110 and 110 '. Coupled to the coupling holes 111 and 111 '.
  • one end of the three-dimensional mesh structure (120, 120 ') may be provided with connecting portions 140, 140' for coupling to the coupling holes (111, 111 ').
  • the connecting portions 140 and 140 ' may have a predetermined length and protrude from one end of the three-dimensional mesh structure 120 and 120', and the one or more three-dimensional mesh structures 120 and 120 'may be provided.
  • the coupling parts 140 and 140 ′ may be detachably installed at the coupling parts 110 and 110 ′.
  • connection parts 140 and 140 'and the coupling holes 111 and 111' are formed in shapes and sizes corresponding to each other.
  • one or more of the three-dimensional mesh structure 120, 120 ' may be spaced apart at equal intervals on a plane perpendicular to the rotation axis (130, 130').
  • four three-dimensional mesh structures are arranged at intervals of 90 degrees, but the number and placement angle are not limited thereto.
  • the one or more three-dimensional mesh structure (120, 120 ') is detachably installed from the coupling portion (110, 110'), it can be easily replaced as necessary.
  • one or more three-dimensional mesh structure is detachable to the impeller, a chain reaction may be carried by carrying a different material on each of the one or more three-dimensional mesh structures, and various applications may be possible.
  • an enzyme may be immobilized to an arbitrary first mesh structure among the plurality of three-dimensional mesh structures 120 coupled to the impeller 100, and an antibody may be immobilized to another arbitrary second mesh structure.
  • the reaction may be simultaneously performed with stirring.
  • ABS acrylonitrile-butadiene-styrene
  • PS-PSMA polystyrene-polystyrene-co-maleic anhydride
  • the fiber assembly 150 in which the antibody is immobilized in this manner is inserted into the internal space 129 of the support 121.
  • Antigen detection experiment was performed using the three-dimensional mesh structure 120 in which the antibody immobilized fiber aggregate 150 was inserted.
  • four mesh structures were put in 5 ⁇ g / mL of the antigen solution and stirred at 100 rpm. As a result, as can be seen in Figure 12a, it was confirmed that the antigen attached to the antibody over time.
  • the three-dimensional mesh structure 120 ′ according to FIGS. 4 to 6 is the same as the three-dimensional mesh structure 120 according to FIGS. 1 to 3 to manufacture the fiber aggregate 150 ′ from the outside, and then the support body 121 ′. Use the built-in method. However, in the three-dimensional mesh structure 120 according to FIGS. 1 to 3, the antigen is attached to the fiber aggregate, while in the three-dimensional mesh structure 120 ′ according to FIGS. 4 to 6, the enzyme is attached to the fiber assembly 150 ′. Attaching a will be described as an example.
  • the fiber assembly 150 ' is made of polystyrene-polystyrene-co-maleic anhydride (PS-PSMA) and produced by electrospinning.
  • PS-PSMA polystyrene-polystyrene-co-maleic anhydride
  • Immobilization of the enzyme is covalently bonded to the 10 mg / ml GOx solution for about 2 hours to PS-PSMA, and then added to the stirring agent ammonium sulfate to 55% and stirred. GA, the crosslinker, is then added to bring the final concentration to 0.5%.
  • tilt shaking 50 rpm is performed at 4 for 17 hours. This is washed three times for 5 minutes at 200 rpm using a buffer solution.
  • 100 mM Tris solution pH 7.0 was added and stirred at 200 rpm for 1 hour at room temperature.
  • the enzyme-fiber-fiber aggregate 150 ' is synthesized and stored in the buffer solution.
  • a plurality of inner spaces 129a ', 129b', and 129c ' are formed in the assembly of the plurality of plate-like structures 125a', 125b ', 125c', and 125d '.
  • one three-dimensional mesh structure 120 ' is completed.
  • H 2 O 2 was generated using the three-dimensional mesh structure 120 'into which the GOx immobilized fiber aggregate 150' was inserted, and experiments were conducted on the bacterial growth inhibitory effect of the generated H 2 O 2 .
  • H 2 O 2 generation experiment was carried out over time. 10 mM glucose was added and stirred at 400 rpm. As a result, as can be seen in Figure 13a, the mesh impeller inserted the GOx immobilized three-dimensional structure was confirmed to continuously generate H 2 O 2 .
  • the three-dimensional mesh structure of the impeller according to the embodiment of the present invention has a three-dimensional mesh shape, and the organic catalyst, the inorganic catalyst, and the biomolecules are highly concentrated on the surface and the inside of the three-dimensional mesh structure. Because it reacts with, there is an advantage that the reaction can proceed at a high rate compared to the prior art.
  • the fiber aggregates are embedded in each of the plurality of internal spaces formed between the plurality of plate-like structures, thereby improving the immobilization amount of materials such as organic catalysts, inorganic catalysts and biomolecules in the fiber aggregates.
  • one or more three-dimensional mesh structure is detachable to the impeller, it is possible to carry out a chain reaction by supporting a different material on each of the three-dimensional mesh structure provided with one or more.

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  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un corps de structure maillé et sur une hélice ayant un corps de structure maillé. Un corps de structure à maillage tridimensionnelle selon le mode de réalisation de la présente invention peut comprendre: un support ayant une structure à maillage tridimensionnelle; et un ensemble fibreux intégré dans le support et pouvant être mis en contact avec un premier matériau.
PCT/KR2016/006866 2015-06-25 2016-06-27 Corps de structure à maillage tridimensionnelle et turbine ayant un corps de structure à maillage tridimensionnelle WO2017209341A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20150090713 2015-06-25
KR20150091394 2015-06-26
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KR1020160066338A KR101830198B1 (ko) 2015-06-25 2016-05-30 바이오분자-고분자섬유 복합체 및 이의 제조방법
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KR200361879Y1 (ko) * 2004-06-22 2004-09-13 최정희 폐수처리용 미생물 담체
KR200417037Y1 (ko) * 2006-03-03 2006-05-23 유덕환경(주) 호기성 기능과 혐기성 기능을 동시에 갖는 기능성 담체유닛
KR101394558B1 (ko) * 2012-08-24 2014-05-14 대송환경개발(주) 오폐수처리장치용 미생물 접촉여재 고정유닛
KR20160058579A (ko) * 2014-11-17 2016-05-25 주식회사 아모그린텍 기능성 멤브레인 및 그의 제조 방법

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KR100244367B1 (ko) * 1997-09-10 2000-02-01 양인모 생물학적 수처리방법
KR200361879Y1 (ko) * 2004-06-22 2004-09-13 최정희 폐수처리용 미생물 담체
KR200417037Y1 (ko) * 2006-03-03 2006-05-23 유덕환경(주) 호기성 기능과 혐기성 기능을 동시에 갖는 기능성 담체유닛
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