WO2017137494A1 - Préparation de matériel de paroi cellulaire de carotte - Google Patents

Préparation de matériel de paroi cellulaire de carotte Download PDF

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Publication number
WO2017137494A1
WO2017137494A1 PCT/EP2017/052861 EP2017052861W WO2017137494A1 WO 2017137494 A1 WO2017137494 A1 WO 2017137494A1 EP 2017052861 W EP2017052861 W EP 2017052861W WO 2017137494 A1 WO2017137494 A1 WO 2017137494A1
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WO
WIPO (PCT)
Prior art keywords
cell wall
wall material
preparation
carrot
carrot cell
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PCT/EP2017/052861
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English (en)
Inventor
Elisabeth Cornelia M. BOUWENS
Hendrikus Theodorus W. M. Van Der Hijden
Michael Jacobus SUIJKER
Original Assignee
Unilever N.V.
Unilever Plc
Conopco, Inc., D/B/A Unilever
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever N.V., Unilever Plc, Conopco, Inc., D/B/A Unilever filed Critical Unilever N.V.
Priority to EP17703185.3A priority Critical patent/EP3414279A1/fr
Priority to BR112018016251A priority patent/BR112018016251A2/pt
Priority to EA201891819A priority patent/EA201891819A1/ru
Publication of WO2017137494A1 publication Critical patent/WO2017137494A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H8/00—Macromolecular compounds derived from lignocellulosic materials
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L1/00—Compositions of cellulose, modified cellulose or cellulose derivatives
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/14—Hemicellulose; Derivatives thereof

Definitions

  • the present invention relates to a preparation of carrot cell wall material and a method for preparing a preparation of carrot cell wall material. It also relates to a composition comprising an aqueous phase and a preparation of carrot cell wall material and a method to prepare such a composition. The invention also provides use of a preparation of carrot cell wall material to modify the rheological properties of an aqueous phase and to use of such a preparation to structure an aqueous phase.
  • compositions comprising an aqueous phase are desirable in many applications. This particularly holds true for food compositions. Many foodstuffs have or require a structured aqueous phase. Some foodstuffs have thickened or well-suspending water phases by their very nature, whereas in others consumer appreciation or product stability (for example against sedimentation or coalescence) depend on additional means to control their rheology.
  • Denatured, gelatinised starch is a well-known structurant for aqueous systems.
  • Citrus fibres are also known as structuring agents for aqueous phases. However, these often require extensive processing before they are suitable for use as structuring agents. Such processing is relatively costly and may reduce the perceived naturalness of a composition comprising such fibres.
  • Other cellulose-based, plant-derived structuring agents have also been developed. However, such materials often exhibit related issues.
  • WO 2014/142651 discloses the use of particulate cellulose material derived from vegetable pulp - in particular from sugar beet pulp - for many different types of rheological modification, but the material requires chemical and or enzymatic treatment to degrade and or extract pectin and hemicellulose.
  • WO 2014/147393 discloses a process for preparing cellulose containing particles from plant material using a peroxide agent. The bleaching step is believed to partially degrade pectins and hemicelluloses.
  • compositions in particular food compositions comprising an aqueous phase wherein the aqueous phase has controllable rheological properties, is structurable, and wherein the structurant is plant- based.
  • carrot cell wall material with excellent structuring and/or rheology-modifying characteristics can be prepared from carrots, provided that solutes and other non-cell wall components are removed from the cell wall material to a sufficient degree.
  • Such a preparation of carrot cell wall material can be prepared using a relatively simple process that does not depend on enzymatic treatment or bleaching.
  • a carrot cell wall material that is characterised in terms of its particle size and in terms of the ratios of several saccharide monomers of the fibrous material making up the cell wall material was found to be highly suitable to structure aqueous phases, tailor their rheological properties and/or control their suspending properties.
  • the invention provides a preparation of carrot cell wall material, wherein:
  • the cell wall material is in particulate form and comprises particles having a size of between 25 and 500 ⁇ ;
  • the cell wall material has a ratio of galacturonic acid monomers to bound glucose monomers of at most 1 .0;
  • the cell wall material has a ratio of galactose monomers to bound glucose
  • a preparation of carrot cell wall material displaying the above desirable characteristics can be made by a relatively simple process directed at removing solutes and other non-cell-wall components from the cell wall material by a method involving heating and pureeing followed by a washing step. Therefore, according to a second aspect, the invention provides a method for preparing a preparation of carrot cell wall material according to the invention, wherein the method includes the steps of
  • the present invention provides a preparation of carrot cell wall material obtainable by the method according to the invention.
  • the preparation of carrot cell material of the present invention is highly suitable to modify the rheological properties of aqueous phases. Therefore, according to a fourth aspect, the present invention provides a composition comprising an aqueous phase and the preparation of carrot cell wall material according to the invention, wherein the preparation is dispersed in said aqueous phase.
  • the present invention provides a method for preparing a composition comprising a structured aqueous phase, including the step of dispersing the preparation of carrot cell wall material according to the invention into an aqueous medium so as to form said structured aqueous phase.
  • the present invention provides use of a preparation of carrot cell wall material according to the invention in a composition comprising an aqueous phase to modify the rheological properties of said aqueous phase.
  • the present invention provides use of a preparation of carrot cell wall material according to the invention in a composition comprising an aqueous phase to structure said aqueous phase.
  • FIGURE 1 provides a CSLM micrograph of the carrot cell wall material of Example 18.
  • FIGURE 2 provides a CSLM micrograph of the carrot cell wall material of Example 19.
  • FIGURE 3 provides a CSLM micrograph of the carrot cell wall material of Example 21.
  • any feature of one aspect of the present invention may be utilised in any other aspect of the invention.
  • the word “comprising” is intended to mean “including” but not necessarily “consisting of or “composed of.” In other words, the listed steps or options need not be exhaustive. It is noted that the examples given in the description below are intended to clarify the invention and are not intended to limit the invention to those examples per se. Similarly, all percentages are weight/weight percentages unless otherwise indicated. Moreover, weight percentage (wt. %) is based on the total weight of the product unless otherwise stated. Except in the operating and comparative examples, or where otherwise explicitly indicated, all numbers in this description indicating amounts of material or conditions of reaction, physical properties of materials and/or use are to be understood as modified by the word "about”.
  • the present invention relates to a preparation of carrot cell wall material.
  • carrot cell wall material is understood as material derived from the cell walls of the tissue of carrot (i.e. the taproot of Daucus carota).
  • cell wall material is predominantly made up of cellulose, hemicellulose and pectin.
  • the cell wall material is in particulate form and comprises particles having a size of between 25 and 500 ⁇ .
  • the particle size is determined by wet sieving.
  • a particle size below 500 ⁇ means that the particles pass a sieve with an aperture size of 500 ⁇ .
  • a particle size above 25 ⁇ means that a particle does not pass a sieve with a pore size of 25 ⁇ .
  • wet sieving an aqueous dispersion of the cell wall material at a suitable concentration is passed through a sieve, as detailed hereinbelow.
  • cell wall material within the size ranges is capable of acting as a rheology modifier by virtue of its ability to build extended networks throughout an aqueous phase. Fragments that are too large do not significantly contribute to such network formation. Moreover they may impart other undesirable characteristics, as they may lead to a grainy, gritty structure. Especially for applications in food products, these would be undesirable.
  • the preparation of carrot cell wall material preferably does not contain large amounts of particles having a size outside the specified range. Therefore, preferably at least 80 wt-%, more preferably at least 90 wt-% and even more preferably at least 95 wt-% and still more preferably more preferably at least 98 wt-% by dry weight of the carrot cell wall material in the preparation passes a sieve of 500 ⁇ pore size.
  • the cell wall material comprises particles having a size not more than 400 ⁇ , more preferably not more than 300 ⁇ , even more preferably not more than 200 ⁇ and still more preferably not more than 150 ⁇ .
  • at least 80 wt-% by dry weight of the carrot cell wall material in the preparation passes a sieve of 400 ⁇ pore size, more preferably of 300 ⁇ , even more preferably of 200 ⁇ and still more preferably of 150 ⁇ pore size.
  • Cell wall fragments that are below the lower size limit are believed to be too small to provide the desired benefits as they cannot contribute to the network formation.
  • particles including one or more intact cell wall enclosures and/or one or more fragments of such enclosures that still retain their native spheroidal or cotyloid shape are particularly effective in imparting the desired rheological modifications. Therefore particles comprising such intact enclosures and/or cotyloid fragments are preferred.
  • typical carrot cells have a diameter of about 40 micrometres.
  • fragments of cell wall material with a diameter of about 25 ⁇ are believed to sufficiently retain the cotyloid shape related to the excellent structuring properties.
  • the advantageous curvature associated with intact cell wall enclosures or cotyloid fragments is believed to be non-discernible when cell wall fragments are much smaller than about 25 ⁇ in size. Such small fragments typically become more platelet-like.
  • Suitable sieves include for example stainless steel woven wire laboratory test sieves of the appropriate aperture size available from Endecotts Ltd (London, England) as described in the Methods hereinbelow. Separation of particles of carrot cell wall material with sizes above and below 25 ⁇ , respectively, may also suitably be carried out using a filter cloth with the appropriate pore size, for example a Miracloth filter (Calbiochem) as described in the Methods hereinbelow.
  • a filter cloth with the appropriate pore size for example a Miracloth filter (Calbiochem) as described in the Methods hereinbelow.
  • At least 50 wt-% of the particles comprise one or more intact cell wall enclosure, more preferably at least 75 wt-% and even more preferably at least 90 wt-%, wherein the percentage is by dry weight of the particles.
  • intact cell wall enclosures are understood to be enclosures that retain the spheroidal shape of the original carrot cells, though the solutes and other cell-constituents have been removed at least in part.
  • the presence of intact cell wall enclosures in the preparation of carrot cell wall material can easily be observed using microscopic techniques that are suitable for visualising cell walls as known to the skilled person. Confocal scanning microscopy is an example of such a technique as explained hereinbelow.
  • the particle size of the cell wall material can for instance be controlled by conventional methods known to the skilled person, including well-established comminution methods and size selection methods.
  • the advantageous rheological or structuring properties of the preparation of cell wall material of the present invention is related to the ratio of the galacturonic acid and the glucose monomers present in the preparation.
  • the carrot cell wall material in the preparation has a ratio of galacturonic acid monomers to bound glucose monomers of at most 1 .0, wherein the monomer ratio is by weight of the monomers.
  • the ratio of galacturonic acid monomers to bound glucose monomers is preferably at most 0.98, more preferably at most 0.95, and even more preferably at most 0.91 by weight of the monomers.
  • the monomer ratio can be determined by a method based on NMR spectroscopy and Saeman hydrolysis as explained in the Methods section hereinbelow.
  • bound glucose monomers refer to those glucose monomers that are part of the polysaccharides in the cell wall material. Such bound glucose is predominantly present in the cellulose and hemicellulose.
  • the amount of bound glucose monomers may be lower than the total amount of glucose monomers, because by nature, carrots typically also comprise mono- and oligosaccharides, such as glucose and sucrose.
  • the ratio of galacturonic acid to bound glucose of the cell wall material in the present preparation is lower than that in native, untreated carrot cell wall material. The desired ratio can easily be arrived at by the method according to the second aspect of the present invention. It is believed that galacturonic acid monomers are predominantly present in the pectin contained in the cell wall material.
  • the bound glucose is predominantly present in the cellulose and hemicellulose.
  • the ratio of pectin with regard cellulose and hemicellulose decreases during manufacture of the preparation of carrot cell wall material of the present invention.
  • Other solutes are believed that owing to its greater water-solubility, the ratio of pectin with regard cellulose and hemicellulose decreases during manufacture of the preparation of carrot cell wall material of the present invention.
  • solute content of the carrot cell wall material can suitably be quantified in terms of the conductivity of a dispersion of the cell wall material, since such conductivity typically depends on the level of water-soluble electrolytes in the preparation of carrot cell wall material.
  • an aqueous dispersion comprising the carrot cell wall material in an amount corresponding to a dry matter content of 1 .00 wt-% with regard to the total dispersion
  • said dispersion has a conductivity at 20 °C of at most 300 ⁇ / ⁇ (microsiemens per centimeter), more preferably at most 200 ⁇ 8/ ⁇ and even more preferably at most 100 ⁇ / ⁇ , yet more preferably at most 50 ⁇ 8/ ⁇ " ⁇ , still more preferably at most 20 ⁇ / ⁇ and even still more preferably at most 15 ⁇ /cm, wherein the dispersion essentially consists of the preparation of carrot cell wall material and demineralised water.
  • solute content of the carrot cell wall material can also suitably be quantified in terms of its Brix value. Therefore, it is preferred that upon preparation of an aqueous dispersion comprising the carrot cell wall material in an amount
  • said dispersion has a Brix value of 2 °Brix or less, more preferably 1 °Brix or less, even more preferably 0.2 °Brix or less, and still more preferably 0.1 °Brix or less, wherein the Brix value is determined by the refractive index-based method as known to the skilled person.
  • the advantageous rheology-modifying and structuring properties of the preparation of carrot cell wall material can surprisingly be obtained under relatively mild conditions, without the need of an intensive bleaching step. It is believed that typical bleach treatments, such as those in WO 2014/147393, lead to substantial degradation of hemicellulose. Consequently, the ratio of galactose monomers to bound glucose monomers is very low in such bleached material, since galactose monomers are predominantly present in hemicellulose. In contrast to such bleached materials, the cell wall material of the present preparation of carrot cell wall material has a ratio of galactose monomers to bound glucose monomers of at least 0.15, wherein the monomer ratio is by weight of the monomers.
  • the ratio of galactose to bound glucose is preferably at least 0.17, more preferably at least 0.20, and even more preferably at least 0.25 by weight of the monomers.
  • the preparation of carrot cell wall material of the present invention preferably has a ratio of galacturonic acid monomers to bound glucose monomers of at least 0.45, more preferably at least 0.50, and even more preferably at least 0.55, by weight of the monomers. Consequently, the carrot cell wall material preferably has a ratio of galacturonic acid monomers to bound glucose monomers of between 0.98 and 0.45, more preferably between 0.95 and 0.50, and even more preferably between 0.91 and 0.55 by weight of the monomers.
  • the preparation of carrot cell wall material of the present invention preferably has a ratio of arabinose monomers to bound glucose monomers of at least 0.10, more preferably at least 0.12, even more preferably at least 0.14 and still more preferably at least 0.16 by weight of the monomers.
  • These monomer ratios can be determined by the same method as the ratio of galacturonic acid to bound glucose.
  • Beta-carotene is the compound that gives most Daucus carota cultivars their characteristic orange colour. It is believed that during typical methods for making the preparation of carrot cell wall material of the present invention, the solute removal also affects the level of beta-carotene in the preparation. Therefore, the preparation of cell wall material preferably comprises beta-carotene in an amount of at most 0.04 wt-%, more preferably at most 0.03 wt-%, even more preferably at most 0.02 wt-%, still more preferably at most 0.01 wt-%, even still more preferably at most 0.005 wt-% and yet more preferably at most 0.004 wt-% with respect to the dry weight of the preparation.
  • the preparation of cell wall matter preferably comprises beta-carotene in an amount of at least 0.0001 wt-%, more preferably at least 0.001 wt-% with respect to the dry weight of the preparation.
  • the preparation preferably comprises between 0.04 wt-% and 0.0001 wt-%, more preferably between 0.03 wt-% and 0.001 wt-% of beta-carotene with respect to the dry weight of the preparation.
  • the preparation of carrot cell wall material according to the invention is in dry form.
  • the preparation in dry form need not be free of water, but has a dry appearance. Consequently, the preparation of carrot cell wall material preferably comprises 15 wt-% or less, more preferably 12 wt-% or less, and even more preferably 10 wt-% or less of water, with regard to the total weight of the preparation.
  • the dry matter content of the preparation in dry form is preferably at least 85 wt- %, more preferably at least 90 wt-% by weight of the total preparation.
  • the preparation comprises the carrot cell wall material in an amount of at least 50 wt-%, more preferably at least 75 wt-%, even more preferably at least 90 wt-%, and still more preferably at least 95 wt-%, wherein the weight percentage is the percentage of the dry weight of the carrot cell wall material with respect to the total dry weight of the preparation.
  • the preparation in dry form is particularly advantageous from a perspective of storage stability and weight efficiency during transport and storage.
  • the advantageous properties of the preparation relate to its behaviour in dispersions, typically aqueous dispersions. Therefore, the preparation in dry form is preferably such that it easily regains its rheology modifying or structuring properties upon redispersing in a liquid medium, in particular water.
  • the preparation in dry form is typically obtainable by a process involving a drying step. Such a drying step is preferably carried out in such a way that the beneficial properties of the preparation are retained. It is believed that the structuring and or rheology-modifying properties are optimally retained if the drying is carried out whilst collapse of the intact cell wall enclosures and cotyloid fragments is minimised or even avoided.
  • the outer volume of the intact cell wall enclosures is retained with regard to the volume of the enclosures before the drying step. It is even more preferred that the particles of carrot cell wall material are redispersible in water by the application of only mild shear, for example by stirring or by agitation using equipment like a Silverson blender.
  • Suitable drying steps to obtain the preparation of the present invention in dry form include techniques like spray drying, freeze-drying, or drying methods based on solvent-exchange.
  • the preparation of carrot cell wall material in dry form comprises the carrot cell wall material in an amount of at least 50 wt-%, more preferably at least 75 wt-%, even more preferably at least 90 wt-%, and still more preferably at least 95 wt-%, wherein the weight percentage is the percentage of the dry weight of the carrot cell wall material with respect to the total dry weight of the preparation. It is even more preferred that the dry matter of the preparation of carrot cell wall material in dry form essentially consists of carrot cell wall material.
  • the preparation in dry from may also comprise an additive directed at retaining the non-collapsed structure of the cell wall material during drying or at facilitating its redispersion upon use. Suitable additives are known to the skilled person.
  • the additive is suitable for use in food applications.
  • it is preferred for the preparation in dry form that re- dispersion of the preparation of carrot cell wall material in water using the Standard Silverson redispersion treatment defined hereinbelow so as to form an aqueous dispersion comprising the preparation of carrot cell wall material in an amount of 1.00 percent by weight of dry material with respect to the total weight of the dispersion yields a shear storage modulus G' of at least 50 Pa, more preferably at least 100 Pa even more preferably at least 200 Pa, and still more preferably at least 500 Pa for said aqueous dispersion, wherein G' is measured as G'(5 min eq.) as defined herein.
  • aqueous dispersion comprising the preparation of carrot cell wall material in an amount of 0.3 percent by weight of dry material with respect to the total weight of the dispersion yields a self-suspending capacity after 24 hours of at least 25%, more preferably at least 50%, even more preferably at least 70%, and still more preferably at least 80% for said aqueous dispersion.
  • the preparation of carrot cell wall material according to the invention is in wet form.
  • the preparation typically comprises one or more solvents, including water.
  • the dry matter content of the preparation in wet form may vary.
  • the preparation may e.g. be in the form of a relatively dilute aqueous dispersion of carrot cell wall material, but it may also be in the form of a concentrated slurry (such as may be formed when the cell wall matter is washed and filtered) or for instance a relatively dense pellet formed during
  • the preparation of carrot cell wall material when in wet form preferably comprises between 0.1 wt-% and 25 wt-%, more preferably between 0.25 wt-% and 20 wt-%, even more preferably between 0.5 wt-% and 15 wt-%and still more preferably between 1 wt-% and 10 wt-% of carrot cell wall material in terms of dry weight with respect to the total weight of the preparation.
  • the preparation of carrot cell wall material of the present invention is in the form of an aqueous dispersion of the cell wall particles.
  • the preparation of carrot cell wall material is in the form of an aqueous dispersion
  • it preferably comprises between 0.1 wt-% and 15 wt- %, more preferably between 0.25 wt-% and 13 wt-%, even more preferably between 0.5 wt-% and 10 wt-% and still more preferably between 1 wt-% and 5 wt-% by dry weight of the carrot cell wall material with respect to the total weight of the preparation.
  • the preparation of carrot cell wall material in wet form is in the form of a concentrated slurry, a filtration residue or a centrifuge pellet
  • it preferably comprises between 5 wt-% and 25 wt-%, more preferably between 10 wt-% and 23 wt-%, even more preferably between 15 wt-% and 20 wt-% by dry weight of the cell wall material with respect to the total weight of the preparation.
  • the preparation in wet form may suitably be applied to structure or rheologically modify aqueous phases by dispersing the preparation therein. Therefore, it is preferred for the preparation in wet form, that dispersion of the preparation of carrot cell wall material in water using the Standard Silverson redispersion treatment defined hereinbelow so as to form an aqueous dispersion comprising the preparation of carrot cell wall material in an amount of 1 .00 percent by weight of dry material with respect to the total weight of the dispersion yields a shear storage modulus G' of at least 50 Pa, more preferably at least 100 Pa, even more preferably at least 200 Pa and still more preferably at least 500 Pa for said aqueous dispersion, wherein G' is measured as G'(5 min eq.) as defined herein.
  • aqueous dispersion comprising the preparation of carrot cell wall material in an amount of 0.3 percent by weight of dry material with respect to the total weight of the dispersion yields a self-suspending capacity after 24 hours of at least 25 %, more preferably at least 50 %, even more preferably at least 70%, and still more preferably at least 80% for said aqueous dispersion.
  • the present invention relates to a method for preparing a preparation of carrot cell wall material according to the first aspect of the invention, comprising the steps detailed hereinabove.
  • the carrot may be provided in any form that is suitable for subjecting to the heat treatment of step b. Therefore, the carrot is preferably provided in sliced or diced or shredded form.
  • the carrot starting material may be subjected to such a slicing/dicing or shredding treatment directed specifically at making it suitable for the present application, but may also be the result of earlier treatments. The latter may for instance be the case if the carrot is sourced from a waste stream such as peelings, scratchings, or press-cakes. From a sustainability point of view it is preferred that the carrot is sourced from a waste stream of another process, in particular another process directed at obtaining a food product or beverage from carrot.
  • Step b of the method includes a heat treatment at a temperature of between 85°C and 1 10°C for a period of at least 20 minutes.
  • the temperature is between 90 °C and 105°C, more preferably between 92°C and 100° and even more preferably between 93°C and 98°C.
  • the heat treatment is preferably for a period of between 20 and 45 minutes.
  • the carrot material should be subjected to a pureeing step c before said washing step.
  • the carrot material may be subjected to the pureeing step before or after the heat treatment.
  • said pureeing may already have taken place in the step of providing the carrot. Pureeing may be carried out by any suitable pureeing equipment as known to the skilled person, including for example Kenwood or Waring or Thermomix blenders, or Silverson mixers.
  • the washing step d is directed at removing solutes and other non-cell wall components from the carrot cell wall material to a sufficient degree.
  • the liquid/solid separation treatment may be any suitable separation treatment known to the skilled person.
  • the washing treatment may be a treatment that is suitable for batch-wise or for continuous operation. Depending on the type of washing, a single step of contacting with water and subsequent separation may suffice, whilst in other types, the washing needs to be repeated in order to obtain a preparation of carrot cell wall material according to the present invention.
  • the washing step is preferably carried out, optionally repeatedly, until the wash water that is separated from the carrot cell wall material has a Brix level of not more than 2 °Brix and/or a conductivity at 20°C of not more than 300 ⁇ 8/ ⁇ " ⁇ .
  • said Brix level is not more than 1 °Brix, more preferably not more than 0.2 °Brix and even more preferably not more than 0.1 °Brix, wherein the Brix is determined by the refractive index method as known to the skilled person.
  • the conductivity of the wash water that is separated from the carrot cell wall material not only depends on the level of solutes present in the carrot material, but also on the background conductivity of the water before it is contacted with the carrot material. Typical process water has a conductivity at 20 °C of between 300 ⁇ / ⁇ and 50 ⁇ 8/ ⁇ " ⁇ .
  • the washing step is carried out, optionally repeatedly, until the conductivity of the wash water after separation has a conductivity at 20°C that is no more than 150%, more preferably no more than 120%, even more preferably no more than 1 10% and still more preferably no more than 105% of the conductivity of the water before contacting it with the carrot material.
  • the washing step is carried out, optionally repeatedly, until the conductivity of the wash water after separation has a conductivity at 20°C of at most 200 ⁇ /cm, more preferably at most 100 ⁇ 8/ ⁇ " ⁇ , yet more preferably at most 50 ⁇ /cm, still more preferably at most 20 ⁇ 8/ ⁇ and even still more preferably at most 15 ⁇ /cm.
  • the method for preparing the preparation of carrot cell wall material may suitably include more steps between the initial heating step and the final washing step. Such additional steps may include subjection to one or more comminution treatments, one or more additional heat treatments and one or more additional washing treatments, for instance in order to arrive at a preparation with the appropriate particle size and with the appropriate ratio of galacturonic acid monomers to bound glucose monomers and the approriate ratio of galactose monomers to bound glucose monomers.
  • the method preferably includes one or more additional steps in which the carrot cell wall material is subjected to a comminution treatment and wherein these comminution treatments take place between said heating step and the last washing step.
  • the additional steps may be present in any suitable order and number. Suitable
  • comminution steps may for example include relatively low shear blending, pureeing, milling or grinding treatments, or high shear treatments, including for instance high pressure homogenisation.
  • the method for preparing the preparation of carrot cell wall material may also include additional steps after the final washing step.
  • the method may optionally include a comminution step after the final washing step, for example in order to reduce the size of the particles to the required size.
  • the method typically also includes a drying step.
  • a drying step is preferably carried out in such a way that the beneficial properties of the preparation are retained. It is believed that the structuring and or rheology-modifying properties are optimally retained if the drying is carried out whilst collapse of the intact cell wall enclosures and cotyloid fragments is minimised or even avoided.
  • Suitable drying steps to obtain the preparation of the present invention in dry form include techniques like spray drying or freeze-drying.
  • such a drying step may include a solvent-exchange process, including the steps of
  • the non-aqueous water-miscible solvent preferably is a solvent in which the cell wall material is poorly dispersible.
  • the solvent is an alcohol, more preferably it is ethanol or isopropanol or a mixture thereof.
  • a solvent mixture that is sufficiently enriched in non-aqueous solvent (and
  • the preparation of carrot cell wall material according to the invention is preferably obtainable by the method according to the second aspect of the invention.
  • compositions comprising the preparation
  • the invention provides a composition comprising aqueous phase and the preparation of carrot cell wall material according to the invention, wherein the preparation is dispersed in said aqueous phase.
  • the composition may be in any suitable format.
  • the preparation of carrot cell wall material is particularly suitable for use in edible compositions. Therefore, the composition preferably is a food composition. It is preferred that the composition, in particular when it is a food composition, is a composition of liquid, gelled, paste-like, spoonable, or semi-solid consistency.
  • the food composition may for example be a soup, sauce, dressing, condiment, beverage, paste, ice cream, desert, or a dairy-based product.
  • the preparation of carrot cell wall material is highly suitable to modify the rheological properties of the aqueous phase.
  • the preparation of the invention is highly suitable as a texture-modifier, which can impart an aqueous phase or entire product with the desired textural properties, including for instance
  • the aqueous phase in the composition preferably is a structured aqueous phase.
  • the composition preferably comprises the preparation of carrot cell wall material in an amount of between 0.1 wt% and 10 wt%, more preferably between 0.2 and 5 wt%, even more preferably between 0.3 and 4 wt% and still more preferably between 0.4 and 3 wt% with regard to the weight of the aqueous phase.
  • the present invention also relates to a method for preparing a composition comprising a structured aqueous phase, including the step of dispersing the preparation of carrot cell wall material according to the invention into an aqueous medium so as to form said structured aqueous phase.
  • any feature that is preferred in the preparation according to the first aspect, the method for preparing the preparation according to the second aspect, or the composition according to the third aspect of the invention is likewise preferred in this method according to the fourth aspect of the invention.
  • the use according to the sixth aspect preferably is use of a preparation of carrot cell wall material according to the invention in a composition comprising an aqueous phase to provide said aqueous phase with a shear storage modulus of at least 50 Pa more preferably at least 100 Pa, even more preferably at least 200 Pa and still more preferably at least 500 Pa, wherein the shear storage modulus is measured as G'(5 min eq) as described herein.
  • the equilibration step the sample is subjected to a time sweep test at very low oscillatory shear for 5 min, the so-called equilibration time, at 1 Hz and a 0.1 % strain.
  • the G' value after 5 min equilibration is taken as value called G' (5 min eq).
  • Miracloth filter (from Calbiochem, supplied by Millipore, Typical pore size: 22-25 ⁇ , composed of rayon-polyester with an acrylic binder) was used for washing carrot cell wall material in water and obtaining a dispersion with wherein the particles of carrot cell wall material are larger than 25 ⁇ " ⁇ . This was done by first adding 500g of a processed slurry of carrot cell wall material (between 1 -4% w/w) on the Miracloth filter using a Buchner funnel and adding portions of about 500ml demineralised water on top of the slurry and gently mixing the carrot cwm and water using a spoon. The filtrate was eluted by gravity.
  • the washing of the cell wall material was done until the filtrate became almost colourless (about 5L of demineralised water was used for washing).
  • the filtrate was removed and the residue was used further.
  • the particle size of the cell wall material was measured by filtration of a dispersion of carrot cell wall material in water through a sieve with 0.500 mm aperture size (Stainless steel woven wire laboratory test sieve from Endecotts Ltd, London, England). Filtration of a dispersion of carrot cwm (e.g.
  • 1 .5 % DM was performed by adding portions of about 500ml demineralised water on top of the slurry and gently mixing the carrot cwm and water using a spoon. The filtrate was eluted by gravity. The % residue was analysed based on % dry weight of the residue divided by the total dry weight.
  • the samples containing the dispersed cwm were centrifuged for 30min at 6000rpm. A volume of 10 ml of the supernatant was then used to measure the conductivity using a standard bench top conductivity meter (Schott, model CS 855).
  • the Brix value of a sample was measured using a digital Pocket refractometer PAL1 -RI (Atago, Tokyo, Japan). By applying about 0.3 mL of sample onto the measuring surface, the instrument displays the refractive index, the Brix and the temperature of the sample. Dry matter
  • DM dry matter
  • Insoluble solids were measured after samples were centrifuged (20000 g for 30 min at room temperature, e.g. 23°C), obtained pellets were exposed overnight to a temperature of 80°C in an oven. The remaining was weighed.
  • the % w/w IS is the ratio of insoluble solid content (g) to the total weight of the sample (g) times 100%.
  • 0.2M phosphate buffer 0.2M KH 2 P0 4 Na2HP04 in D 2 0, pD 7.4 containing 0.1 % NaN 3 .
  • EDTA-d i2 solution 50 mg of EDTA-d i2 (1.5) in 10 ml of D 2 0 (1 .1 ).
  • the probe was tuned to detect 1 H resonances at 600.25 MHz.
  • the internal probe temperature was set to 298K.
  • 32 scans were collected in 57K data points with a relaxation delay of 10 seconds, an acquisition time of 4 seconds and a mixing time of 100 ms.
  • Low power water suppression (16 Hz) was applied for 0.99 seconds.
  • the data were processed in TOPSPIN software version 3.5 pi 1
  • the monomer composition of the polysaccharides in the preparation of carrot cell wall material was determined using a quantitative 1 H-NMR method.
  • the NMR quantification is done on samples which were freeze dried and powdered if needed and which were hydrolysed using the Saeman hydrolysis (Saeman, J.F., Moore, W.E., Mitchell, R.L and Millett, M.A., (1954) Tappi Journal, 37, 336-343).
  • the N MR and the hydrolysis method is applied as described by de Souza et al. (De Souza, A.S., Rietkerk, T., Selin, C.G. and Lankhorst, P.P., (2013). "A robust and universal NMR method for the
  • Deuterated water (D20, 99.85% D), Eurisotop; Deuterated sulphuric acid ( ⁇ 99%), Aldrich; D-(+)-Mannose ( ⁇ 99%), Fluka; D-(+)-Glucose ( ⁇ 99%), Sigma; D-(+)-Galactose ( ⁇ 99%), Sigma; L-Rhamnose ( ⁇ 99%), Aldrich; D-Galacturonic acid (97%), Sigma, L-(+)- Arabinose, ( ⁇ 99%) Sigma, D-(+)-Xylose ( ⁇ 99%) Sigma, Maleic Acid (99.78 ⁇ 0.08 (g/g)%), Fluka.
  • Apparatus Avance I I I 600 MHz NMR spectrometer equipped with a 5mm-cryoprobe, (Bruker GmbH, Germany). (Multipoint) magnetic stirrer, type poly 15, 10 W,
  • Preparation of the polysaccharide sample solution (including solubilization): Weigh 15- 25 mg of each sample in a 15 mL glass tubes. Add 1 mL of 72 (w/w)%. D 2 S0 4 in D 2 0 to each sample.
  • Solubilization Add a stirring bar to each sample. Stir the samples at room temperature for 60 minutes using a multipoint stirrer (the glass tubes are placed in glass vials for stability). After the solubulization step, add 6.2 mL D 2 0 to each sample in order to obtain a final D 2 S0 4 concentration of 14 (w/w) % in D 2 0.
  • NMR Sample preparation Carefully add 1 mL of maleic acid internal standard solution (0.5 mg/ml in D 2 0) and stir the solutions at a magnetic stirrer for 5 minutes. The NMR samples were transferred to 3-mm NMR tubes for analysis.
  • NMR Sample preparation 500 ⁇ of maleic acid internal standard solution was added to the samples. The solutions were stirred for 5 minutes on a magnetic stirrer. The NMR samples were transferred to 3-mm NMR tubes for analysis.
  • a small library was created for the deconvolution of galacturonic acid, galactose, arabinose, glucose, xylose, mannose and rhamnose.
  • Each of the compounds was characterized by a set of spin particles and parameters for chemical shifts, coupling constants, line widths and intensities (populations). These parameters were obtained by fitting experimental spectra of the pure model compounds recorded in D20 using the PERCH NMR software. All parameters were iteratively optimized.
  • the internal standard (IS) consisting of maleic acid was used to calculate the absolute compound concentrations from the fitted populations. The spin particles of the internal standard were included in each fit.
  • MS monosaccharide sugar recovery standard (non-hydrolysed)
  • ⁇ MMPP — UD MM -' I[ -T
  • the GalA Glu ratio is calculated from the galacturonic acid amount in the sample after Saemen hydrolysis [GalA] divided by the total amount of Glucose in the sample
  • Free glucose (free Glu) analysed is the sum of the monomeric glucose in the sample without hydrolysis and the glucose content in the quantified sucrose in the sample without hydrolysis.
  • the glucose amount in sucrose is 50%.
  • Glucose monomeric Glucose monomeric determined for the non-hydrolysed sample
  • Glucose from sucrose The glucose content in sucrose as determined for the non-hydrolysed sample
  • Glucose total giucose-free glucose
  • CSLM Scanning confocal laser microscopy
  • the Square Hole High Shear ScreenTM square hole screen, used as pre-treatment for 5-10min at 3000-5000rpm.
  • the Standard Silverson redispersion treatment used herein was was performed by combining the amounts of a sample of a preparation of carrot cell wall material and demineralised water required to yield 500 grams of a dispersion comprising 1.00 wt% by weight of dry matter of the carrot cell wall material to the total weight of the composition.
  • the combined mixture treated with a Silverson L4RT homogenizer for 10 min at 6000 rpm using workhead 2 (fine emulsor screen) and a 1 L laboratory beaker.
  • Carrots from a local supermarket were peeled and cut in approximatively 1 -2 cm slices. After washing, the slices were heated for 30 minutes in water at 95°C. Then after cooling, the pieces were mechanically disrupted in (one part of) demineralized water using a blender (Kenwood Chef Glass liquidiser) at the maximum speed for 1 to 3 min.
  • the resulting carrot puree was diluted in water to a total solids content of 1 .7 wt-% (Comparative Ex. A).
  • water was added to the undiluted puree (of comparative A) and the resulting slurry was centrifuged for 30 minutes at 4500 rpm at room temperature, yielding a pellet of cell wall material.
  • Example 2 a slurry of carrot cell wall material, prepared according to Ex. 1 , was mechanically processed using an overhead mixer (Silverson L4RT-A mixer, workhead 2, fine emulsor screen with 2 mm diameter round holes during 10 min. at 5000 rpm) and subsequently diluting the slurry to 1.0% total solids content (Ex. 2).
  • the Total solids (TS) were determined by drying a known amount of the carrot puree (comparative A) or the pellet (Ex. 1 ) in an oven at 105°C, during 16h under 100 mbar vacuum. The TS was determined on an average of three measurements.
  • the pellet was resuspended in a total volume of 8800 mL and homogenised using a Silverson BX mixer with a slotted disintegrator work head (workhead 3) in a 10L plastic vessel for 5 minutes at 3000 rpm.
  • the homogenized slurry was washed two more times with a centrifugation time of 20 minutes at 8450g.
  • the Brix value of the supernatant of the final washing was ⁇ 0.1 °.
  • the resulting cell wall material was split in 400 g batches and different batches were redispersed in demineralised water at a concentration of 0.8, 1.2 and 1.7 % DM, respectively.
  • the batches were redispersed with a Silverson mixer (with 2 mm square hole screen, workhead 1 ) for 8 min at 8000rpm (Ex. 3).
  • the results summarised in Table 2 demonstrate that good structuring can also be obtained with a preparation of carrot cell wall material according to the invention that was prepared using frozen carrot press cake as the starting material.
  • Table 2 Ex 3 storage modulus as function of concentration and homogenization method
  • Frozen carrot press cake (375g ex Van Rijsingen) was allowed to thaw and 3000 g of boiled demineralised water was added.
  • the suspension was heated in the microwave for 5min at 1000W and subsequently blended using a Thermomix (30min at 90 °C, speed 4, ex Vorwerk, from Thermomix Benelux NV CNUDDE)).
  • the suspension was then centrifuged using a Beckman Coulter Avanti J-26S XP centrifuge, with 6x 400g in centrifuge buckets, for 30rminut.es at 10000g.
  • the resulting pellet was re-dispersed in demi water to a total volume of 1600 mL and centrifuged another time to remove more soluble compounds.
  • the freeze-dried powder was rehydrated in demineralized water at 1 .50 % DM of carrot cwm and blended in a Waring blender for 5 min at speed 3 and 100 ml was washed using filtration on Miracloth (pore size 25 ⁇ , from CalBiochem). A small sample was taken to measure %DM and to determine the yield. The residue was found to contain 75% of the DM of the filtered carrot cwm. The residue was mixed in demineralized water at a DM content of 1 .5% and the carrot cwm samples (before and after filtration) storage moduli were measured after 24 h storage (Table 4).
  • Example B was made by taking 50 g FW (fresh weight) of fine cut raw carrot (from van Rijsingen, stored frozen) and drying the frozen carrot using freeze drying. The freeze dried carrot was milled to a powder using a Waring Laboratory Blender for 2 min at speed 4.
  • Example C was made by taking 50 g FW (fresh weight) of fine cut raw carrot (from van Rijsingen, stored frozen), letting it thaw for 15 min at room temperature and then washing the carrot at room temperature on a Miracloth filter (poresize 25 ⁇ , from CalBiochem) with an excess of demineralised water until the filtrate was almost colourless. The residue was collected and freeze dried and then milled to a powder using a Waring Laboratory Blender for 2 min at speed 4.
  • a Waring Laboratory Blender for 2 min at speed 4.
  • Example 6 154g of fine cut raw carrot (from van Rijsingen) was used which was mixed with 1.5L of boiled demineralised water and heated in the microwave for 5min at 1000W. The suspension was then blended using a Thermomix (30min at 90 °C speed 4). The suspension was then washed using a Buchner funnel and a Miracloth filter (25 ⁇ pore size, from CalBiochem). Demineralised water (around 4L) was used for washing the fibers until the filtrate was almost transparent. After being washed, the residue left on the filter was collected and freeze dried and then milled to a powder using a Waring Laboratory Blender for 2 min at speed 4.
  • a Waring Laboratory Blender for 2 min at speed 4.
  • Example 7 was made similar to Example 6, but after the washing step the carrot residue was collected and demineralised water was added to prepare a suspension of 1 % DM. Then the carrot suspension was treated by shear using a bench top Silverson (L4RT) for 10 min 3000rpm using the square hole screen workhead 1 (large pores) followed by 10 min 6000 rpm using the fine emulsor screen workhead 2. The suspension was then washed using a Buchner funnel and a Miracloth filter (25 ⁇ pore size, from CalBiochem). Demineralised water (around 4L) was used for washing the fibers until the filtrate was almost transparent. After being washed, the residue left on the filter was collected and freeze dried and then milled to a powder using a Waring Laboratory Blender for 2 min at speed 4.
  • L4RT bench top Silverson
  • Demineralised water around 4L was used for washing the fibers until the filtrate was almost transparent.
  • Example 8 was made similar as described for Example 7 except that after the Silverson treatment a HPH treatment was applied using the lab scale Panda Plus High Pressure Homogeniser (from Niro Soavi) between 600-1000 bar. Then the HPH-treated carrot suspension was frozen at -80 °C, then freeze dried, then milled to a powder using a Waring Laboratory Blender for 2 min at speed 4.
  • a HPH treatment was applied using the lab scale Panda Plus High Pressure Homogeniser (from Niro Soavi) between 600-1000 bar. Then the HPH-treated carrot suspension was frozen at -80 °C, then freeze dried, then milled to a powder using a Waring Laboratory Blender for 2 min at speed 4.
  • Example 9 To 125g of fine cut raw carrot (from van Rijsingen) 625 g boiled
  • demineralised water was added and heated in the microwave for 5min at 1000W. Subsequently the suspension was blended using a Thermomix (30min at 90 °C speed 4). Washing of the fibers was carried out using a Buchner funnel and a Miracloth filter (25 ⁇ pore size). Demineralised water ( ⁇ 4L) was used for washing the fibers until the filtrate was almost transparent. The washed residue on the filter was collected and demineralised water was added to reach a total weight of 1 .3kg.
  • This suspension was homogenized with a Silverson mixer (L4RT) firstly during 5 minutes at 3000 rpm (using workhead 1 , square hole screen pores) followed by 10 min 7700 rpm (using workhead 2, fine emulsor screen). Subsequently the homogenised carrot preparation was heated in the Thermomix during 30min at 90 °C (speed 4) and homogenized with a Silverson mixer (L4RT) for 10 min at 7700 rpm (using workhead 2, fine emulsor screen) and then washed (filtration 25 micro meter pore size) and homogenised in a Niro Soavi High Pressure Homogeniser at 1000-1600 bar.
  • a Silverson mixer L4RT
  • Carbohydrate composition of freeze dried carrot samples was determined after Seaman hydrolysis by NMR (Table 6).
  • Free glucose content in sample F and G were respectively 10.58 and 9.46% (w/w), whereas the free glucose content in the other samples was below 0.01 % (w/w).
  • Examples according to the invention have a GalA:Glu ratio below 1 .0, whereas the comparative examples that did not undergo essential unit operations have ratios below 1.0.
  • Examples 10 and Comparative Examples D to G were performed to assess the effect of hydrogen peroxide bleaching on the monomer composition of carrot cell wall material.
  • Pieces of carrot tissue were mixed with hot water to a dry matter content of 5% (w/v). The mixture was heated in the microwave to reach 90C. Subsequently the suspension was blended using a Thermomix (120 min at 90 °C speed 3-4). Then Silverson treatment for 5 min using workhead 1 , square hole screen pores) at 6000 rpm followed by 10 min at 7700 rpm (using workhead 2, fine emulsor screen).
  • Example 10 was incubated as Ex. G, but in the absence of H2O2.
  • the samples were freeze-died prior to further analysis.
  • the monosaccharide composition of the freeze dried samples was determined after Seaman hydrolysis by NMR as described in the Method section. (Table 8). ⁇ -carotene content was also determined by NMR.
  • the GahGlu ratios of the comparative sample treated with H2O2 are below 0.15 whereas the GahGlu ratios of the Examples according to the invention are above 0.15.
  • the residues after washing of the cwm of example 9 and of the comparative examples D-G were collected and suspended to a 1 % cell wall material (cwm). Physical properties (rheology, yield, pH) were measured (Table 9). Table 9.
  • Preparations of carrot cell wall material were prepared using the unit operations heating, blending, washing and mechanical shearing as described in Ex. 1 , but applied in different order as presented in Table 10.
  • the heating step was not the first one, carrot slices were blanched to inactivate enzymes and mechanically disrupted in water (ratio carrot/water: 1/1 ) using the Kenwood blender at maximum speed for 2 min. Dispersions were diluted to 1.0% total solid content before determination of the storage modulud G'.
  • Example 18 An aliquot of 300g of this suspension was homogenized with a Silverson mixer (L4RT) firstly during 5 minutes at 3000 rpm using a ring with large pores (workhead 1 , square hole screen) and subsequently during 10min at 7700rpm using a ring with small pores (workhead 2, emulsor screen) (Example 18; H+W+S).
  • Example 19 H+W+S+H+S
  • Example 19 (H+W+S+H+S) was freeze dried and resuspended in water and homogenized with a Silverson (L4RT) for 10 min at 6000 rpm (workhead 2, emulsor screen) to obtain example 20 (H+W+S+H+S+FD).
  • sample 19 H+W+S+H+S+W+S+HPH
  • Miracloth filter 25 ⁇ pore size
  • the resuspended pellet was homogenized with a Silverson mixer (L4RT) for 10 min at 7700 rpm (workhead 2, emulsor screen) followed by the shear treatment in a High Pressure Homogeniser (HPH) using a lab scale Panda Plus HPH (from Niro Soavi) between 600-1600 bar.
  • HPH High Pressure Homogeniser
  • CSLM images of Figures 1 -3 show that all samples contained recognizable cell walls, including intact cell wall enclosures and cotyloid cell wall fragments and that increased shear leads to smaller particle size. It was found that the majority of the particles had a size below 500 ⁇ as not more than 2% by dry weight of the cell wall material was retained on a 500 ⁇ sieve (woven wire stainless steel sieve from Endecotts).
  • the self-suspending capacity of the above carrot cell wall preparations was determined in a graduated cylinder of a 0.3% (w/v) on dry weight basis in demineralized water. As a control the starting carrot material was diluted to a content of 0.3% insoluble cell wall material in demineralized water.
  • the Silverson-homogenized carrot cwm from Ex. 3a was centrifuged and the pellet was mixed with a solution of MaltoDextrin 20 (MD20) 50% w/w in a 10L stainless steel bucket to obtain a 1 :1 ratio of cwm:MD20 on a dry matter base.
  • the mixture was centrifuged and the pellet was spread on metal trays and cooled to -20 a -30°C using a blast freezer within 1 hr and were subsequently dried at -20°C in a freeze dryer.
  • the cake was broken into 1 -2 cm pieces (manually) and filled into the blender reservoir till half volume.
  • the cake pieces were pulverised using a blender using short bursts at low speed counting up to 10-15 s.
  • the preparation of carrot cell wall material of Example 2 was used to structure a tomato-based sauce.
  • 30% and 70% (respectively) of the tomato paste (28-30 °Brix) was replaced by 52 and 122 g of a 5.0% (DM) the preparation of cwm, respectively, to obtain 0.52 and 1 .22% of carrot cwm material content on a dry weight basis (Table 14).
  • the sauce was prepared by mixing the ingredients with a household mixer. Rheological and other physical properties of the sauces were determined (Table 15).
  • Example 26 Oil in water emulsion containing carrot cwm
  • the preparation of carrot cell wall material from Ex. 3a was used to structure an oil in water emulsion.
  • the dried preparation was rehydrated in water and mixed with the egg for 30 seconds. After 20 sec the water phase was added and mixed. Then oil was added and the mixture was emulsified in Fryma Delmix.

Abstract

La présente invention concerne une préparation de matériel de paroi cellulaire de carotte, ledit matériel de paroi cellulaire étant sous forme particulaire et comprenant des particules ayant une taille comprise entre 25 et 500 μm, le matériel de paroi cellulaire présentant un rapport en poids de monomères d'acide galacturonique aux monomères de glucose lié inférieur ou égal à 1,0 ; et la matériel de paroi cellulaire présentant un rapport de monomères de galactose aux monomères de glucose lié supérieur ou égal à 0,15, et les rapports des monomères étant en poids. L'invention concerne également un procédé permettant de préparer la préparation de matériel de paroi cellulaire de carotte. L'invention concerne également une composition comprenant une phase aqueuse et une préparation de matériel de paroi cellulaire de carotte, ainsi qu'un procédé permettant de préparer une telle composition. L'invention concerne également l'utilisation d'une préparation de matériel de paroi cellulaire de carotte pour modifier les propriétés rhéologiques d'une phase aqueuse et l'utilisation d'une telle préparation pour structurer une phase aqueuse.
PCT/EP2017/052861 2016-02-09 2017-02-09 Préparation de matériel de paroi cellulaire de carotte WO2017137494A1 (fr)

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EP17703185.3A EP3414279A1 (fr) 2016-02-09 2017-02-09 Préparation de matériel de paroi cellulaire de carotte
BR112018016251A BR112018016251A2 (pt) 2016-02-09 2017-02-09 preparação de material de parede celular de cenoura, método para preparação de material de parede celular de cenoura, composição, método para preparação de uma composição compreendendo uma fase aquosa estruturada e uso de uma preparação de material de parede celular de cenoura em uma composição
EA201891819A EA201891819A1 (ru) 2016-02-09 2017-02-09 Препарат материала клеточной стенки моркови

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005107500A1 (fr) * 2004-05-07 2005-11-17 Forskarpatent I Syd Ab Graisses vegetales de substitution pour produits carnes
JP2007051075A (ja) * 2005-08-16 2007-03-01 Keio Gijuku 植物体抽出物調製方法、並びに植物体抽出物及びその用途
US20080233238A1 (en) * 2007-02-08 2008-09-25 Grimmway Enterprises, Inc. Supercritical co2 carrot feedstock extraction
EP2462817A1 (fr) * 2010-12-09 2012-06-13 Provalor BV Procédé de fabrication de purée et purée de carottes
US20140056946A1 (en) * 2011-04-29 2014-02-27 Unilever N.V. Method for isolation of polysaccharides
WO2014142651A1 (fr) 2013-03-15 2014-09-18 Koninklijke Coöperatie Cosun U.A. Stabilisation de particules solides en suspension et/ou de bulles de gaz dans des fluides aqueux
WO2014147393A1 (fr) 2013-03-18 2014-09-25 Cellucomp Limited Matière particulaire à base de cellulose
WO2015130163A1 (fr) * 2014-02-28 2015-09-03 Marinus Jacobus Vervoort Procédé d'obtention d'ingrédients naturels pour des produits alimentaires par liaison de l'eau
WO2015128155A1 (fr) * 2014-02-28 2015-09-03 Unilever N.V. Préparations de grappes de parois cellulaires parenchymateuses végétales

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005107500A1 (fr) * 2004-05-07 2005-11-17 Forskarpatent I Syd Ab Graisses vegetales de substitution pour produits carnes
JP2007051075A (ja) * 2005-08-16 2007-03-01 Keio Gijuku 植物体抽出物調製方法、並びに植物体抽出物及びその用途
US20080233238A1 (en) * 2007-02-08 2008-09-25 Grimmway Enterprises, Inc. Supercritical co2 carrot feedstock extraction
EP2462817A1 (fr) * 2010-12-09 2012-06-13 Provalor BV Procédé de fabrication de purée et purée de carottes
US20140056946A1 (en) * 2011-04-29 2014-02-27 Unilever N.V. Method for isolation of polysaccharides
WO2014142651A1 (fr) 2013-03-15 2014-09-18 Koninklijke Coöperatie Cosun U.A. Stabilisation de particules solides en suspension et/ou de bulles de gaz dans des fluides aqueux
WO2014147393A1 (fr) 2013-03-18 2014-09-25 Cellucomp Limited Matière particulaire à base de cellulose
WO2015130163A1 (fr) * 2014-02-28 2015-09-03 Marinus Jacobus Vervoort Procédé d'obtention d'ingrédients naturels pour des produits alimentaires par liaison de l'eau
WO2015128155A1 (fr) * 2014-02-28 2015-09-03 Unilever N.V. Préparations de grappes de parois cellulaires parenchymateuses végétales

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DE SOUZA, A.S.; RIETKERK, T.; SELIN, C.G; LANKHORST, P.P.: "A robust and universal NMR method for the compositional analysis of polysaccharides", CARBOHYDRATE POLYMERS, vol. 95, 2013, pages 657 - 663, XP028534188, DOI: doi:10.1016/j.carbpol.2013.02.036
SAEMAN, J.F.; MOORE, W.E.; MITCHELL, R.L; MILLETT, M.A., TAPPI JOURNAL, vol. 37, 1954, pages 336 - 343

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