WO2017097013A1

WO2017097013A1 - Wholly natural functional drink for removing harmful reactive oxygen species from human bodies, and preparation method thereof

Info

Publication number: WO2017097013A1
Application number: PCT/CN2016/100012
Authority: WO
Inventors: 张贯京; 陈兴明; 高伟明
Original assignee: 深圳市贝沃德克生物技术研究院有限公司
Priority date: 2015-12-10
Filing date: 2016-09-24
Publication date: 2017-06-15
Also published as: CN105532996A

Abstract

Disclosed are a wholly natural functional drink for removing harmful reactive oxygen species from a human body, and preparation method thereof. The wholly natural functional drink consists of the following components in part by weight: a green tea extract: 0.5 to 2 parts; a grape skin extract: 0.1 to 0.5 part; a mulberry leaves extract: 0.1 to 0.5 part; a wild chrysanthemum extract: 0.05 to 0.2 part; a gingko leaf extract: 0.05 to 0.2 part; a natural flavor: 0.5 to 2 parts; a natural sweetener: 0.5 to 2 parts; and water making up the remaining composition.

Description

Inventive name: pure natural functional beverage for removing harmful active oxygen in human body and preparation method thereof

Technical field

[0001] The present invention relates to the field of life and health, and in particular to a pure natural functional beverage for removing harmful active oxygen from the human body and a preparation method thereof.

Background technique

[0002] Reactive Oxygen (ROS) contains a compound of superoxide radical 02- and hydrogen peroxide H202, which is a by-product of aerobic respiration in biological mitochondria, and its accumulation in cells forms oxidation. Stress, which damages lipids, proteins, and nucleic acids, thereby damaging cells. This long-term cumulative oxidative stress is a major cause of human aging and many metabolic diseases. The superoxide free radical 02-activity in the human body is very strong. Hydrogen peroxide H202 has a long period of time in the human body. No matter which one of the two substances exists, it has a great toxic effect on the human body. At present, most of the antioxidant drinks at home and abroad are prepared by adding compounds, and the functional beverages extracted naturally are rare. In addition, the existing functional beverages can only remove one of superoxide radical 02- and hydrogen peroxide H202, but cannot achieve double scavenging of superoxide radical 02- and hydrogen peroxide H202, so it cannot be effectively removed. Harmful active oxygen in the human body, its antioxidant effect is not obvious.

technical problem

[0003] The main object of the present invention is to provide a compound composition for removing harmful active oxygen in human body and a preparation method thereof, aiming at solving the current antioxidant-containing beverage containing additives and failing to realize superoxide radical 02- in active oxygen of human body. And the double removal of hydrogen peroxide H202 can not completely eliminate the problem of harmful active oxygen species in the human body.

Problem solution

Technical solution

[0004] In order to achieve the above object, the present invention provides a pure natural functional beverage for removing harmful active oxygen from the human body, the pure natural functional beverage being composed of the following parts by weight: green tea extract: 0.5 to 2 parts, Grape Skin extract: 0.1~0.5 parts, Mulberry leaf extract: 0.1~0.5 parts, Wild chrysanthemum extract: 0.05~0.2 parts, Ginkgo biloba extract: 0.05~0.2 parts, Natural flavor: 0.5~2 servings, Natural sweet Flavor: 0.5~2 parts, the rest is water.

[0005] Preferably, the natural flavoring agent is one or more of a navel orange extract, an osmanthus extract, a dragon fruit extract, a kiwi extract, and a blueberry extract.

[0006] Preferably, the natural sweetener is one or more of the palm leaf raspberry extract, the scutellaria extract, and the betel nut extract.

[0007] Preferably, the pure natural functional beverage is composed of the following parts by weight: green tea extract: 0.5 parts, grape skin extract: 0.1 part, mulberry leaf extract: 0.1 part, wild chrysanthemum extract: 0.05 parts , Ginkgo biloba extract: 0.05 parts, natural flavor: 0.5 parts; Natural sweetener: 0.5 parts, water: 98.2 parts.

[0008] Preferably, the pure natural functional beverage is composed of the following parts by weight: green tea extract: 2 parts, grape skin extract: 0.5 parts, mulberry leaf extract: 0.5 parts, wild chrysanthemum extract: 0.2 parts , Ginkgo biloba extract: 0.2 parts, natural flavoring agent: 2 parts, natural sweetener: 2 parts, water: 92.6 parts.

[0009] Preferably, the pure natural functional beverage is composed of the following parts by weight: green tea extract: 1 part, grape skin extract: 0.25 parts, mulberry leaf extract: 0.25 parts, wild chrysanthemum extract: 0.1 part , Ginkgo biloba extract: 0.1 part, natural flavor: 1 part, natural sweetener: 1 part, water: 96.3 parts.

[0010] Preferably, the pure natural functional beverage is composed of the following parts by weight: green tea extract: 1.5 parts, grape skin extract: 0.5 parts, mulberry leaf extract: 0.5 parts, wild chrysanthemum extract: 0.2 parts , Ginkgo biloba extract: 0.2 parts, natural flavor: 1 part, natural sweetener: 2 parts, water: 94.1 parts.

[0011] Preferably, the pure natural functional beverage is composed of the following parts by weight: green tea extract: 1.8 parts, grape skin extract: 0.5 parts, mulberry leaf extract: 0.3 parts, wild chrysanthemum extract: 0.2 parts , Ginkgo biloba extract: 0.2 parts, natural flavor: 1.5 parts, natural sweetener: 1.5 parts, water: 94.0 parts.

[0012] In addition, in order to achieve the above object, the present invention also provides a preparation method of a pure natural functional beverage for removing harmful active oxygen in the human body, and the preparation method comprises the following steps: A. extracting green tea extract and grape skin , mulberry leaf extract, wild chrysanthemum extract, ginkgo biloba extract, natural flavoring agent, natural sweetener and water are mixed and stirred at room temperature according to a preset ratio; B. High-speed centrifugation of the mixture formed in step A Separation, separation liquid standby; C, the separation liquid formed in step B is homogenized by a homogenizer to obtain a homogeneous liquid, the homogenization pressure is 20Mpa; D, using a microporous membrane to filter and sterilize the homogenized liquid, Obtaining the pure natural work Can drink.

[0013] Preferably, the pore size of the microporous membrane is 0.88-0.22 μm.

Advantageous effects of the invention

Beneficial effect

[0014] Compared with the prior art, the pure natural functional beverage for removing harmful active oxygen in the human body, on the one hand, combines the mulberry leaf extract and the grape skin extract according to the weight ratio, the mulberry leaf extract The 7-hydroxycoumarin contained in the cell membrane can activate superoxide dismutase SOD2 through the cell membrane to convert superoxide radical 02- into hydroperoxide 202, and the resveratrol contained in the grape skin extract can also be penetrated. The cell membrane activates catalase to catalyze the conversion of hydrazine hydrate 202 to hydrazine 20, thereby achieving double scavenging of superoxide radical 02- and hydrazine hydride 202, ensuring optimal oxidation resistance. On the other hand, natural functional beverages do not contain any food additives or food preservatives. The main ingredients are all natural botanical ingredients. Long-term use of the pure natural functional beverages of the present invention can significantly reduce superoxide radicals 02- and peroxidation in human blood. The content of hydroquinone 202, so as to achieve the body's antioxidant effect, easy to obtain the favor of consumers, and low cost, low price, in line with the general public demand for pure natural food, has greater market value.

BEST MODE FOR CARRYING OUT THE INVENTION

The present invention will be further described in detail below with reference to specific embodiments, which are illustrative of the present invention, and the present invention is not limited to the following. Example.

[0016] The pure natural functional beverage provided by the invention for removing harmful active oxygen in the human body adopts the following weight ratio:

[0017] Green tea extract: 0.5~2 parts, grape skin extract: 0.1~0.5 parts, mulberry leaf extract: 0.1~0.5 parts, wild chrysanthemum extract: 0.05~0.2 parts, Ginkgo biloba extract: 0.05~0.2 , natural flavoring agent: 0.5~2 servings

, natural sweetener: 0.5~2 servings, the rest of the composition is water.

[0018] Among the above components, the tea polyphenol EGCG contained in the green tea extract has the same effect of purifying superoxide 02- and peroxide oxime 202; the mulberry leaf extract contains 7-hydroxycoumarin, It has the effect of activating superoxide dismutase 2 (SOD2) in the human body and scavenging superoxide 02-; grape skin extract contains Resveratrol, which has the function of activating catalase (Peroxiredoxins) in human cells and degrading hydrogen peroxide H202; wild chrysanthemum extract is rich in vitamin C and has activating catalase to scavenge hydrogen peroxide H202 The effect of Ginkgo biloba extract contains glycosides, which can induce the expression of antioxidant enzymes GST-P1 and GCLC, and directly remove superoxide anion 02-; natural flavors contain a lot of vitamin C and carotene, which can not only increase The aroma of the beverage, and has the effect of scavenging hydrogen peroxide H20 2; the natural sweetener can improve the mouthfeel of the beverage, and the vitamin C contained therein has the peroxide peroxide H202.

[0019] In the above embodiment, the natural flavoring agent is one or more of navel orange extract, sweet-scented osmanthus extract, dragon fruit extract, kiwi fruit extract, blueberry extract; The extracts extracted from the above-mentioned fruits with different aromas can make the human body healthier by using natural flavoring agents, and can avoid the harm of the existing chemical synthetic flavoring agents to the drinker. The natural sweetener is one or more of the extract of the palm leaf raspberry extract, the extract of the genus Corydalis, and the extract of the horse areca. The natural sweetener is a pure natural extract.

[0020] In the above examples, Ginkgo biloba extract may also be used as a natural preservative to ensure the shelf life of the purely functional beverage of the present invention.

[0021] The pure natural functional beverages prepared in the following Examples 1-8 for removing harmful active oxygen in the human body are all taken as weights of 100 g (ie, equivalent to 100 parts by weight), and the parts by weight of the pure natural functional beverages. The allocation ratio is described in detail below.

Embodiment 1

[0023] The components of the first preferred embodiment of the natural-natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0024] Green Tea Extract: 0.5g, Grape Skin Extract: 0.1g, Mulberry Leaf Extract: 0.1g, Wild Chrysanthemum Extract: 0.05g, Ginkgo Biloba Extract: 0.05g, Natural Flavor: 0.5g, Natural Sweetener: 0.5g, 7X: 98.2g

[0025] Example 2

The components of the second preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0027] Green tea extract: 2g, grape skin extract: 0.5g, mulberry leaf extract: 0.5g, wild chrysanthemum extract: 0. 2g, Ginkgo biloba extract: 0.2g, natural flavoring agent: 2g, natural sweetener: 2g, water: 92.6g.

Embodiment 3

[0029] The components of the third preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0030] Green Tea Extract: lg, Grape Skin Extract: 0.25g, Mulberry Leaf Extract: 0.25g, Wild Chrysanthemum Extract

: 0.1g, Ginkgo biloba extract: 0.1g, natural flavoring agent: lg, natural sweetener: lg, water: 96.3g.

Example 4

[0032] The components of the fourth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body of the present invention are as follows:

[0033] Green Green Tea Extract: 2g, Grape Skin Extract: lg, Mulberry Leaf Extract: 0.5g, Wild Chrysanthemum Extract: 0

.lg, Ginkgo biloba extract: 0.1g, natural flavoring agent: 2g, natural sweetener: 2g, water: 92.3g.

Example 5

[0035] The components of the fifth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0036] Green Tea Extract: lg, Grape Skin Extract: 0.5g, Mulberry Leaf Extract: 0.5g, Wild Chrysanthemum Extract: 0. lg, Ginkgo Biloba Extract: 0.2g, Natural Flavor: lg, Natural Sweet Flavor: 2g, 7j: 94.7g.

Example 6

[0038] The components of the sixth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0039] Green Tea Extract: 1.5g, Grape Skin Extract: 0.5g, Mulberry Leaf Extract: 0.5g, Wild Chrysanthemum Extract:

0.2 g, Ginkgo biloba extract: 0.2 g, natural flavoring agent: lg, natural sweetener: 2 g, 7j: 94.1 g.

Example 7

[0041] The components of the seventh preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body of the present invention are as follows:

[0042] Green Tea Extract: 1.8g, Grape Skin Extract: 0.5g, Mulberry Leaf Extract: 0.3g, Wild Chrysanthemum Extract: 0.2g, Ginkgo Biloba Extract: 0.2g, Natural Flavor: 1.5g, Natural Sweetener: 1.5g, water: 94.0g.

Embodiment 8

[0044] The components of the eighth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention as follows:

[0045] Green tea extract: lg, grape skin extract: 0.4 g, mulberry leaf extract: 0.5 g, wild chrysanthemum extract: 0.

2g, Ginkgo biloba extract: 0.1g, natural flavoring agent: lg, natural sweetener: lg, water: 95.8g.

[0046] The purely natural functional beverages for removing harmful active oxygen in the human body in the above Examples 1-8 were prepared by the following methods:

[0047] A, green tea extract, grape skin extract, mulberry leaf extract, wild chrysanthemum extract, ginkgo biloba extract, natural flavor, natural sweetener and water at a predetermined ratio of stirring at room temperature Mixing to form a mixture;

[0048] B, the mixture formed in step A is centrifuged at high speed to obtain a separation solution for use;

[0049] C, the separation liquid formed in step B is homogenized by a homogenizer to obtain a homogeneous liquid, the homogenization pressure is 20Mpa;

[0050] D. Filtering and sterilizing the homogenized liquid by using a microporous membrane to obtain the pure natural functional beverage.

In the above embodiment, the pore diameter of the microporous membrane may be selected to be 0.88 to 0.22 μm. The detailed operation of filtering and sterilizing the homogenized liquid by using the microporous membrane in the above step D is as follows:

[0052] (1) assembly, sterilization;

[0053] A microporous membrane (for example, a 0.22 μη pore size microporous membrane) is placed in a clean plastic filter, screwed and flattened, and packaged for sterilization (0.25 MPa, 121.5 ° C sterilization for 20 min) .

[0054] (2) connection;

[0055] Under sterile conditions, the inlet of the sterilized plastic filter is aseptically attached to a syringe containing the solution to be filtered, the needle is attached to the outlet and inserted into a sterile test tube with a rubber stopper.

[0056] (3) pressure filtration;

[0057] pressurizing the solution to be filtered in the syringe and slowly squeezing it into a sterile test tube, filtering, and dispensing the needle

. Pressurize the filter, the force should be appropriate, not too fast and too fast, so as to prevent the bacteria from being squeezed through.

(4) sterility inspection;

[0059] Aseptically aspirate the sterile filtrate in the sterile test tube 0.1ml on the beef paste peptone plate, evenly coated

The cells were cultured in a greenhouse at 37 ° C for 24 hours to check for bacterial growth.

(5) cleaning;

[0061] Remove the microporous membrane on the plastic filter, clean the plastic filter, and replace it with a new microporous membrane

, assembly and dressing, and then use after sterilizing. [0062] The entire operation process should be strictly aseptically operated under aseptic conditions to prevent contamination. Filtration should avoid leakage at the joints.

[0063] Clinical trials Through a health management cloud platform, 15 volunteers were sampled and tested, taking an average of 200 ml of the functional beverage daily. The average range of values of hydrogen peroxide H202 and superoxide 02- before taking was compared according to the population. Each time, hydrogen peroxide H202 and superoxide 02-content were measured intravenously, and averaged three times (averaged by 30 days per month).

[0064] The test results of the pure natural functional beverage of the present invention for removing hydrogen peroxide H202 are as follows:

Hydrogen peroxide content of H202 hydrogen peroxide content of H202 (mM)

[0066] Groups First month before drinking Second month Third month

Example 1 (15 people) 120.78 100.13 92.33 84.6

Example 2 (15 people 126.5 116.0 105.3 95.2

Example 3 (15 people 110.35 96.8 85.4 77.1

Example 4 (15 people 175.27 157.4 137.1 126.8

Example 5 (15 people 90.1 87.8 67.5 71.3)

Example 6 (15 people 95.52 88.9 78.1 67.5

Example 7 (15 people 100.56 99.5 98.4 97.7

Example 8 (15 people 106.5 95.42 83.86 74.3

[0075]

[0076] The test results of the pure natural functional beverage of the present invention for removing superoxide 02- are as follows:

[0077] Superoxide 02- content Superoxide 02- content (mM)

[0078] Groups before drinking the first month, the second month, the third month

Example 1 (15 people) 180.78 160.13 142.33 124.6

Example 2 (15 people) 166.5 146.0 125.3 105.2

Example 3 (15 people) 130.35 116.8 105.4 97.1

Example 4 (15 people) 165.27 157.4 137.1 126.8

Example 5 (15 people) 190.1 187.8 167.5 151.3

Example 6 (15 people) 155.52 128.9 108.1 97.5

Example 7 (15 people) 130.56 109.5 98.4 77.7 Example 8 (15 people) 185.6 178.5 165.3 148.9

[0087]

[0088]

[0089]

[0090] In formulating the pure natural functional beverage of the present invention, comprehensive consideration is given to the various components of active oxygen and food additive. On the one hand, in the beverage formula, the mulberry leaf extract and the grape skin extract are combined according to the weight ratio, and the 7-hydroxycoumarin contained in the mulberry leaves can penetrate the cell membrane, thereby activating the human superoxide dismutase. SOD2 catalysis can only remove superoxide radical 02-converted to hydrogen peroxide H2 02, resveratrol contained in grape skin, can also pass through the cell membrane, activate catalase to catalyze the conversion of hydrogen peroxide H202 to water H20 , thereby achieving a transition from superoxide radical 02- to hydrogen peroxide H202 to harmless water to the human body; green tea extract EGCG can simultaneously purify superoxide radical 02- and hydrogen peroxide H202; containing vitamins . Vitamin E, anthocyanins, glycosides, carotene, osmanthus brass and other natural compounds rich in antioxidant compounds, and activate multiple antioxidant signaling pathways to ensure optimal antioxidant effects. On the other hand, natural functional beverages do not contain any food additives, food preservatives, the main components are all natural botanical ingredients, and can achieve double scavenging of superoxide radical 02- and hydrogen peroxide H202, long-term use of the present invention The pure natural functional beverage can significantly reduce the content of superoxide free radical 02- and hydrogen peroxide H202 in human blood, thereby achieving the anti-oxidation effect of the human body, easily obtaining the favor of consumers, and the cost is low, the price is cheap, and the user is resistant to oxidation and natural. The demand for food has greater market value.

[0091]

The above is only a preferred embodiment of the present invention, and is not intended to limit the scope of the invention, and the equivalent structure or equivalent function changes made by the contents of the specification of the present invention may be directly or indirectly applied to other related technical fields. The same is included in the scope of patent protection of the present invention.

Embodiments of the invention

The present invention will be further described in detail below with reference to specific embodiments, which are illustrative of the invention, and the present invention is not limited to the following. Example. [0094] The pure natural functional beverage provided by the invention for removing harmful active oxygen in the human body adopts the following weight ratio group distribution ratio:

[0095] Green tea extract: 0.5~2 parts, grape skin extract: 0.1~0.5 parts, mulberry leaf extract: 0.1~0.5 parts, wild chrysanthemum extract: 0.05~0.2 parts, Ginkgo biloba extract: 0.05~0.2 , natural flavoring agent: 0.5~2 servings

, natural sweetener: 0.5~2 servings, the rest of the composition is water.

Among the above components, the tea polyphenol EGCG contained in the green tea extract has the same effect of purifying superoxide 02- and peroxide H202; the mulberry leaf extract contains 7-hydroxycoumarin, It has the effect of activating superoxide dismutase 2 (SOD2) in the human body and scavenging superoxide 02-; grape skin extract contains resveratrol, which activates catalase (Peroxiredoxins) in human cells and degrades The effect of hydrogen peroxide H202; Wild chrysanthemum extract is rich in vitamin C, with the effect of catalase to scavenge hydrogen peroxide H202; Ginkgo biloba extract contains glycosides, which can induce antioxidant enzyme GST-P1 And the expression of GCLC, and then directly remove superoxide anion 02-; natural flavor contains a lot of vitamin C and carotene, not only can increase the aroma of the beverage, but also has the effect of scavenging hydrogen peroxide H20 2; natural sweetener can improve The taste of the beverage, which contains vitamin C, has the ability to remove peroxide H202.

[0097] In the above embodiment, the natural flavoring agent is one or more of a navel orange extract, an osmanthus extract, a dragon fruit extract, a kiwi extract, and a blueberry extract; the natural flavoring agent has The extracts extracted from the above-mentioned fruits with different aromas can make the human body healthier by using natural flavoring agents, and can avoid the harm of the existing chemical synthetic flavoring agents to the drinker. The natural sweetener is one or more of the extract of the palm leaf raspberry extract, the extract of the genus Corydalis, and the extract of the horse areca. The natural sweetener is a pure natural extract.

In the above embodiments, the Ginkgo biloba extract may also be used as a natural preservative to ensure the edible shelf life of the purely natural functional beverage of the present invention.

[0099] The purely natural functional beverages prepared in the following Examples 1-8 for removing harmful active oxygen in the human body are each 100 parts by weight (ie, equivalent to 100 parts by weight), for the weight group of the pure natural functional beverages. The allocation ratio is described in detail below.

Embodiment 1

[0101] The components of the first preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention as follows:

Green Tea Extract: 0.5g, Grape Skin Extract: 0.1g, Mulberry Leaf Extract: 0.1g, Wild Chrysanthemum Extract: 0.05g, Ginkgo Biloba Extract: 0.05g, Natural Flavor: 0.5g, Natural Sweetener: 0.5g, 7X: 98.2g

Example 2

[0104] The components of the second preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0105] Green tea extract: 2g, grape skin extract: 0.5g, mulberry leaf extract: 0.5g, wild chrysanthemum extract: 0.

2g, Ginkgo biloba extract: 0.2g, natural flavoring agent: 2g, natural sweetener: 2g, water: 92.6g.

Example 3

[0107] The components of the third preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body of the present invention are as follows:

[0108] Green Tea Extract: lg, Grape Skin Extract: 0.25g, Mulberry Leaf Extract: 0.25g, Wild Chrysanthemum Extract

Example 4

[0110] The components of the fourth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body of the present invention are as follows:

[0111] Green Green Tea Extract: 2g, Grape Skin Extract: lg, Mulberry Leaf Extract: 0.5g, Wild Chrysanthemum Extract: 0

Example 5

[0113] The components of the fifth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body of the present invention are as follows:

Green Tea Extract: lg, Grape Skin Extract: 0.5g, Mulberry Leaf Extract: 0.5g, Wild Chrysanthemum Extract: 0. lg, Ginkgo Biloba Extract: 0.2g, Natural Flavor: lg, Natural Sweet Flavor: 2g, 7j: 94.7g.

Example 6

The components of the sixth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0117] Green Tea Extract: 1.5g, Grape Skin Extract: 0.5g, Mulberry Leaf Extract: 0.5g, Wild Chrysanthemum Extract: 0.2 g, Ginkgo biloba extract: 0.2 g, natural flavoring agent: lg, natural sweetener: 2 g, 7j: 94.1 g.

Example 7

[0119] The components of the seventh preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

Green Tea Extract: 1.8g, Grape Skin Extract: 0.5g, Mulberry Leaf Extract: 0.3g, Wild Chrysanthemum Extract: 0.2g, Ginkgo Biloba Extract: 0.2g, Natural Flavor: 1.5g, Natural Sweetener: 1.5g, water: 94.0g.

Example 8

[0122] The components of the eighth preferred embodiment of the purely natural functional beverage for removing harmful active oxygen in the human body according to the present invention are as follows:

[0123] Green tea extract: lg, grape skin extract: 0.4 g, mulberry leaf extract: 0.5 g, wild chrysanthemum extract: 0.

The purely natural functional beverages for removing harmful active oxygen in the human body in the above Examples 1-8 were prepared by the following methods:

[0125] A. Mixing green tea extract, grape skin extract, mulberry leaf extract, wild chrysanthemum extract, ginkgo biloba extract, natural flavoring agent, natural sweetener and water at a predetermined ratio at room temperature Mixing to form a mixture;

[0126] B, the mixture formed in step A is centrifuged at high speed to obtain a separation liquid for use;

[0127] C, the separation liquid formed in step B is homogenized by a homogenizer to obtain a homogeneous liquid, the homogenization pressure is 20Mpa;

[0128] D. Filtering and sterilizing the homogenized liquid by using a microporous membrane to obtain the pure natural functional beverage.

[0130] (1) assembly, sterilization;

[0131] A microporous membrane (for example, a 0.22 μm pore size microfiltration membrane) is placed in a clean plastic filter, screwed and flattened, and packaged for sterilization (0.25 MPa, 121.5 ° C sterilization for 20 min) .

[0132] (2) connection;

[0133] Under sterile conditions, the inlet of the sterilized plastic filter is aseptically attached to a syringe containing the solution to be filtered, the needle is attached to the outlet and inserted into a sterile test tube with a rubber stopper.

(3) pressure filtration; [0135] pressurizing the solution to be filtered in the syringe and slowly squeezing it into a sterile test tube, filtering, and dispensing the needle

(4) sterility test;

[0137] Aseptically aspirate 0.1 ml of the sterile filtrate in a sterile test tube onto a beef paste peptone plate, evenly spread

[0138] (5) cleaning;

[0139] Remove the microporous membrane on the plastic filter, clean the plastic filter, and replace it with a new microporous membrane.

, assembly and dressing, and then use after sterilizing.

[0140] The entire operation should be strictly aseptically operated under aseptic conditions to prevent contamination. Filtration should avoid leakage at the joints.

[0141] Clinical trials Through a health management cloud platform, 15 volunteers were sampled, and an average of 200 ml of the functional beverage was taken daily. The average range of values of hydrogen peroxide H202 and superoxide 02- before taking was compared according to the population. Each time, hydrogen peroxide H202 and superoxide 02-content were measured intravenously, and averaged three times (averaged by 30 days per month).

The test results of the natural natural functional beverage of the present invention for removing hydrogen peroxide H202 are as follows:

Hydrogen peroxide content of H202 hydrogen peroxide content of H202 (mM)

[0144] Groups First month before drinking Second month Third month

Example 1 (15 persons) 120.78 100.13 92.33 84.6

Example 2 (15 people) 126.5 116.0 105.3 95.2

Example 3 (15 people) 110.35 96.8 85.4 77.1

Example 4 (15 people) 175.27 157.4 137.1 126.8

Example 5 (15 people) 90.1 87.8 67.5 71.3

Example 6 (15 people) 95.52 88.9 78.1 67.5

Example 7 (15 people) 100.56 99.5 98.4 97.7

Example 8 (15 persons) 106.5 95.42 83.86 74.3

[0153]

[0154] The test results of the pure natural functional beverage of the present invention for removing superoxide 02- are shown in Table 2 below:

Superoxide 02- content of superoxide 02- (mM) [0156] The first month before the first month of drinking, the third month of the second month

Example 1 (15 persons) 180.78 160.13 142.33 124.6

Example 2 (15 people) 166.5 146.0 125.3 105.2

Example 3 (15 people) 130.35 116.8 105.4 97.1

Example 4 (15 people) 165.27 157.4 137.1 126.8

Example 5 (15 persons) 190.1 187.8 167.5 151.3

Example 6 (15 people) 155.52 128.9 108.1 97.5

Example 7 (15 people) 130.56 109.5 98.4 77.7

Example 8 (15 persons) 185.6 178.5 165.3 148.9

[0165]

[0166]

[0167]

[0168] In formulating the pure natural functional beverage of the present invention, comprehensive consideration is given to the various components of the active oxygen and the food additive. On the one hand, in the beverage formula, the mulberry leaf extract and the grape skin extract are combined according to the weight ratio, and the 7-hydroxycoumarin contained in the mulberry leaves can penetrate the cell membrane, thereby activating the human superoxide dismutase. SOD2 catalysis can only remove superoxide radical 02-converted to hydrogen peroxide H2 02, resveratrol contained in grape skin, can also pass through the cell membrane, activate catalase to catalyze the conversion of hydrogen peroxide H202 to water H20 , thereby achieving a transition from superoxide radical 02- to hydrogen peroxide H202 to harmless water to the human body; green tea extract EGCG can simultaneously purify superoxide radical 02- and hydrogen peroxide H202; containing vitamins . Vitamin E, anthocyanins, glycosides, carotene, osmanthus brass and other natural compounds rich in antioxidant compounds, and activate multiple antioxidant signaling pathways to ensure optimal antioxidant effects. On the other hand, natural functional beverages do not contain any food additives, food preservatives, the main components are all natural botanical ingredients, and can achieve double scavenging of superoxide radical 02- and hydrogen peroxide H202, long-term use of the present invention The pure natural functional beverage can significantly reduce the content of superoxide free radical 02- and hydrogen peroxide H202 in human blood, thereby achieving the anti-oxidation effect of the human body, easily obtaining the favor of consumers, and the cost is low, the price is cheap, and the user is resistant to oxidation and natural. The demand for food has greater market value.

[0169] The above is only a preferred embodiment of the present invention, and is not intended to limit the scope of the invention, and the equivalent structure or equivalent function changes made by the contents of the specification of the present invention may be directly or indirectly applied to other related technical fields. The same is included in the scope of patent protection of the present invention.

Industrial applicability

Compared with the prior art, the pure natural functional beverage for removing harmful active oxygen in the human body, on the one hand, combines the mulberry leaf extract and the grape skin extract according to the weight ratio, the mulberry leaf extract The 7-hydroxycoumarin contained in the cell membrane can activate superoxide dismutase SOD2 through the cell membrane to convert superoxide radical 02- into hydrogen peroxide H202, and the resveratrol contained in the grape skin extract can also be penetrated. The cell membrane activates catalase to catalyze the conversion of hydrogen peroxide H202 to water H20, thereby achieving double elimination of superoxide radical 02- and hydrogen peroxide H202, ensuring optimal oxidation resistance. On the other hand, natural functional beverages do not contain any food additives or food preservatives. The main ingredients are all natural botanical ingredients. Long-term use of the pure natural functional beverages of the present invention can significantly reduce superoxide radicals 02- and peroxidation in human blood. The content of hydrogen H202, so as to achieve the body's antioxidant effect, easy to obtain the favor of consumers, and low cost, low price, in line with the general public demand for pure natural food, has greater market value.

Claims

Claim

A pure natural functional beverage for removing harmful active oxygen in human body, characterized in that the pure natural functional beverage is composed of the following parts by weight: green tea extract: 0.5~2 parts, grape skin extract: 0.1~0.5 , mulberry leaf extract: 0.1~0.5 parts, wild chrysanthemum extract: 0. 05~0.2 parts, ginkgo biloba extract: 0.05~0.2 parts, natural flavoring agent: 0.5~2 parts, natural sweetener: 0.5~ 2 parts, the rest of the composition is water.

The pure natural functional beverage for removing harmful active oxygen in human body according to claim 1, wherein the natural flavoring agent is navel orange extract, sweet-scented osmanthus extract, dragon fruit extract, kiwi fruit extract, and blueberry extract. One or several of them.

The pure natural functional beverage for removing harmful active oxygen in human body according to claim 1, wherein the natural sweetener is in the extract of the palm leaf raspberry extract, the multi-scented extract, and the betel nut extract. One or several.

The pure natural functional beverage for removing harmful active oxygen in human body according to claim 1, wherein the pure natural functional beverage is composed of the following parts by weight: green tea extract: 0.5 part, grape skin extract: 0.1 part, mulberry leaf extract: 0.1 part, wild chrysanthemum extract: 0.05 parts, ginkgo biloba extract: 0.05 parts, natural flavoring agent: 0.5 part; natural sweetener: 0.5 part, τ: 98.2 parts.

The pure natural functional beverage for removing harmful active oxygen in human body according to claim 1, wherein the pure natural functional beverage is composed of the following parts by weight: green tea extract: 2 parts, grape skin extract: 0.5 parts, mulberry leaf extract: 0.5 parts, wild chrysanthemum extract: 0.2 parts, ginkgo biloba extract: 0.2 parts, natural flavoring agent: 2 parts, natural sweetener: 2 parts, water: 92.6 parts.

The pure natural functional beverage for removing harmful active oxygen in human body according to claim 1, wherein the pure natural functional beverage is composed of the following parts by weight: green tea extract: 1 part, grape skin extract: 0.25 parts, mulberry leaf extract: 0.25 parts, wild chrysanthemum extract: 0.1 part, ginkgo biloba extract: 0.1 part, natural flavoring agent: 1 part, natural sweetener: 1 part, water: 96.3 parts.

A pure natural functional beverage for removing harmful active oxygen from the human body according to claim 1, The natural functional beverage is composed of the following parts by weight: green tea extract

: 1.5 parts, Grape skin extract: 0.5 parts, Mulberry leaf extract: 0.5 parts, Wild chrysanthemum extract: 0.2 parts, Ginkgo biloba extract: 0.2 parts, Natural flavor: 1 part, Natural sweetener: 2 servings , Water: 94.1 parts.

[Claim 8] The pure natural functional beverage for removing harmful active oxygen in the human body according to claim 1, wherein the pure natural functional beverage is composed of the following parts by weight: green tea extract: 1.8 parts, Grape skin extract: 0.5 parts, Mulberry leaf extract: 0.3 parts, Wild chrysanthemum extract: 0.2 parts, Ginkgo biloba extract: 0.2 parts, Natural flavor: 1.5 parts, Natural sweetener: 1.5 parts, Τ: 94.0 Share.

[Claim 9] A method for preparing a purely natural functional beverage for removing harmful active oxygen in a human body according to any one of claims 1 to 8, wherein the preparation method comprises the following steps: The green tea extract, the grape skin extract, the mulberry leaf extract, the wild chrysanthemum extract, the ginkgo leaf extract, the natural flavoring agent, the natural sweetener and the water are stirred and mixed at a predetermined ratio at room temperature to form a mixed solution. Β, the mixture formed in the step Α is centrifuged at a high speed to obtain a separation liquid for use; C. The separation liquid formed in the step B is homogenized by a homogenizer to obtain a homogeneous liquid, and the homogenization pressure is 20 MPa; The homogenized liquid is filtered and sterilized using a microporous membrane to obtain the pure natural functional beverage.

[Claim 10] The method for producing a purely natural functional beverage for removing harmful active oxygen in human body according to claim 9, wherein the pore size of the microporous membrane is 0.88-0.22 μm.