WO2017095216A1 - Composition et procédé de lutte contre les escargots pommes - Google Patents

Composition et procédé de lutte contre les escargots pommes Download PDF

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Publication number
WO2017095216A1
WO2017095216A1 PCT/MY2016/000073 MY2016000073W WO2017095216A1 WO 2017095216 A1 WO2017095216 A1 WO 2017095216A1 MY 2016000073 W MY2016000073 W MY 2016000073W WO 2017095216 A1 WO2017095216 A1 WO 2017095216A1
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WO
WIPO (PCT)
Prior art keywords
surfactant
composition
composition according
saponin
selloa
Prior art date
Application number
PCT/MY2016/000073
Other languages
English (en)
Inventor
AWANG.Rita MUHAMAD
Dzolkhifli OMAR
Anis Syahirah MOKHTAR
Original Assignee
Universiti Putra Malaysia (Upm)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiti Putra Malaysia (Upm) filed Critical Universiti Putra Malaysia (Upm)
Publication of WO2017095216A1 publication Critical patent/WO2017095216A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives

Definitions

  • the present invention relates to a composition and a method for controlling Apple snail Pomace a sp.
  • Apple snail, Pomacea sp. is a freshwater edible snail, which is indigenous to South America, but a successful invasive species in Malaysia. In Malaysia it unfortunately acts as a rice pest, hampering the rice production, especially damaging young seedlings of rice.
  • the apple snail is usually controlled chemical pesticides which often have a low degradability.
  • An environmentally friendly alternative is provided by plant-based preparations, using compounds from plants such as Chenopodium quinoa, Yucca schidigera, Quillaja saponaria, Camellia sinensis, usually locally available plants are used. The necessary requirements are sufficient stability, biodegradability and enviromental friendliness of the preparations. There is a continuing need to find pesticide sources and formulations corresponding to these requirements.
  • the present invention provides a composition for controlling snail of Pomacea sp., which contains saponins from Furcraea selloa, preferably from leaves of Furcraea selloa, water, emulsifier and surfactant, and is in the form of nanoemulsion having an average particle size below 500 nm.
  • the saponins are obtained by methanol extraction of Furcraea selloa, preferably of leaves of Furcraea selloa
  • the saponin in Furcraea selloa was subjected to qualitative and quantitative high- performance liquid chromatography (HPLC) analysis with Photodiode Array detector against the analytical standard of saponin from Sigma- Aldrich, USA.
  • HPLC high- performance liquid chromatography
  • the separation of saponin was performed by using CI 8 (5 ⁇ particle size, 150mm x 4.6mm) column (Sigma-Aldrich, USA) and the detection wavelength was set at 280 nm.
  • the temperature was maintained at 30°C, with the injection volume of 20 ⁇ and flow rate of 1.5 ml/min.
  • the standards and samples were separated using a gradient mobile phase consisted of solvent A (99.5 % water and 0.5 % phosphoric acid) and solvent B (99.5 % methanol and 0.5 % phosphoric acid) with ratio 50: 50 v/v for 10 minutes.
  • the saponins from Furcraea selloa are present as the active ingredient, preferably in an amount of 1 wt. % to 30 wt. %, more preferably 1 wt. % to 20 wt. %.
  • the surfactant is preferably an oleochemical surfactant or non-ionic surfactant and preferably of plant origin.
  • a suitable non-ionic surfactant is castor oil ethoxylate (commercially available, e.g., as Termul 1284).
  • the surfactant is preferably present in an amount of 1 wt. % to 20 wt. %.
  • the nanoemulsion has a surface tension below 50 mN.
  • the present invention further provides a method of controlling snail of Pomacea sp. in rice field, which involves the step of applying a composition according to the present invention to the rice field.
  • the recommended dosage for the invention is 100 to 300 gram of saponin crude extract per hectare.
  • the present invention can be applied by using knapsack sprayer with cone or fan nozzle.
  • composition of the present ivention is nontoxic to humans and animals, biodegradable, environmentally friendly, effective in small quantities, and effective against the apple snail with mean LT 50 values of less than 200 minutes.
  • the composition can be stored for at least 6 months without any significant loss of activity.
  • a the plant that is the source of the active ingredient is available in Malaysia, the present composition does not incur any unnecessary costs and does not pollute the environment by the need of transportation.
  • Fig. 1 shows a ternary phase diagram (Example 2).
  • Fig. 2 shows a chromatogram of standard saponin and Fig. 3 shows a chromatogram of saponin from F rcraea selloa (Example 1).
  • Example 1 Preparation and quantification of saponin from Furcraea selloa
  • the solvent in the filtrate was removed in a rotary evaporator at 45°C to get the crude extract.
  • the crude formed was suspended in 10 millimetres of solvent used and the quantification of saponin was subjected to qualitative and quantitative high-performance liquid chromatography (HPLC) analysis with Photodiode Array detector against the analytical standard of saponin from Sigma- Aldrich, USA.
  • HPLC high-performance liquid chromatography
  • the separation of saponin was performed by using CI 8 (5 ⁇ particle size, 150mm x 4.6mm) column (Sigma-Aldrich, USA) and the detection wavelength was set at 280 nm.
  • the temperature was maintained at 30°C, with the injection volume of 20 ⁇ and flow rate of 1.5 ml/min.
  • the ternary phase diagrams were constructed by using three-component phase diagram system (Murell, J.N. and Jenkins, A.D., 1994. Property of Liquids and Solutions: Second edition. Published by John Wiley & Sons, Chichester) - see Fig. 1.
  • the ratios of surfactant and emulsifier in this experiment were fixed to 1 1 combinations (100:0, 90: 10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, 10:90 and 0: 100).
  • Appropriate amounts of surfactant, emulsifier and water were weighed for a total of 0.5g into 10 ml culture glass tube with cap. Samples observation and experimental measurements were performed at room temperature, 26 ⁇ 1°C. Samples which retained a transparent one-phase appearance after centrifuged were chosen for the next test. The result obtained was used to plot the ternary phase diagram.
  • phase diagram constructed different formulations were selected from nanoemulsion region. Selection of formulation from phase diagram was based on the isotropic whereby the one with narrower isotropic region on the plotted phase diagram was not used for the selection of nanoemulsion formulation.
  • the composition of surfactant: emulsifier: water in TF6 was 10%: 10% : 80%.
  • the selected formulation was subjected to stability, surface tension and particle size measurement.
  • Stability test For this test, selected formulation was centrifuged at 3000 rpm for 30 minutes and was kept at two different temperature storages; room temperature (26 ⁇ 1°C) and 54 °C for four weeks. Subsequently, the formulation was evaluated for the ability to retain transparent one-phase appearance. Formulations which retained transparent one-phase appearance were used to indicate the presence of nanoemulsion. The stability of the formulation for 6-month test was subjected to room temperature (26 ⁇ 1°C). Stability Test
  • Measuring particle size of compound and zeta potential were carried out by using Zetasizer to determine the particle size of saponin.
  • the particle size of the formulations range between 50-500nm which is considered as nanoparticles
  • the TF6 formulation was tested on apple snail with five different concentrations (10 wt. %, 7.5 wt. %, 5 wt. %, 2.5 wt. % and 1 wt. % of the active compound in crude) against niclosamide (recommended rate: 2.8 ml in 500ml of water) and water as positive and negative control.
  • Five snails (one to two centimeters) were placed in plastic container filled with 500 ml of each treatment. Each treatment was replicated four times thus, total number of snails used were 20 per treatment.
  • Completely Randomized Design (CRD) was used as experimental design.
  • the containers were provided with 0.3 grams of rice plant (15-20 days old seedling) for apple snail to consume.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne une composition pour lutter contre les escargots de l'espèce Pomacea, qui contient des saponines provenant de Furcraea selloa, de l'eau, un émulsifiant et un tensioactif, et se présente sous forme de nanoémulsion ayant une taille de particule moyenne inférieure à 500 nm. L'invention concerne également un procédé de lutte contre les escargots de l'espèce Pomacea dans un champ de riz, qui comprend l'étape consistant à appliquer ladite composition sur le champ de riz.
PCT/MY2016/000073 2015-12-01 2016-11-04 Composition et procédé de lutte contre les escargots pommes WO2017095216A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2015704374 2015-12-01
MYPI2015704374 2015-12-01

Publications (1)

Publication Number Publication Date
WO2017095216A1 true WO2017095216A1 (fr) 2017-06-08

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ID=58797466

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2016/000073 WO2017095216A1 (fr) 2015-12-01 2016-11-04 Composition et procédé de lutte contre les escargots pommes

Country Status (1)

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WO (1) WO2017095216A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100015236A1 (en) * 2006-09-14 2010-01-21 Shlomo Magdassi Pesticide nanoparticles obtained from microemulsions and nanoemulsions
US20100041629A1 (en) * 2008-08-15 2010-02-18 Sabine Giessler-Blank Nanoemulsions and Processes for their Preparation, And Their Use as Formulations of Plant Protection Agents and/or Pesticides and/or Cosmetic Preparations
CN101999345A (zh) * 2010-10-20 2011-04-06 周端午 绿色、长效抑菌防霉杀虫溶胶制剂
WO2015179313A1 (fr) * 2014-05-19 2015-11-26 Ecoflora Agro Sas Compositions molluscicides et procédés d'utilisation correspondants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100015236A1 (en) * 2006-09-14 2010-01-21 Shlomo Magdassi Pesticide nanoparticles obtained from microemulsions and nanoemulsions
US20100041629A1 (en) * 2008-08-15 2010-02-18 Sabine Giessler-Blank Nanoemulsions and Processes for their Preparation, And Their Use as Formulations of Plant Protection Agents and/or Pesticides and/or Cosmetic Preparations
CN101999345A (zh) * 2010-10-20 2011-04-06 周端午 绿色、长效抑菌防霉杀虫溶胶制剂
WO2015179313A1 (fr) * 2014-05-19 2015-11-26 Ecoflora Agro Sas Compositions molluscicides et procédés d'utilisation correspondants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OSMAN, GAMALAT Y. ET AL.: "Biological Studies on Biomphalaria Alexandrina Snails Treated with Furcraea Selloa Marginata Plant (family: Agavaceae) and Bacillus Thuringiensis Kurstaki (Dipel-2x).", JOURNAL OF APPLIED PHARMACEUTICAL SEIENCE, vol. 1, no. 10, 2011, pages 47 - 55, XP055387626 *

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