WO2017090408A1

WO2017090408A1 - Additive for equine feed

Info

Publication number: WO2017090408A1
Application number: PCT/JP2016/082838
Authority: WO
Inventors: 数充本多; 敏夫岩田; 健太郎上手; 武土屋; 智幸谷川
Original assignee: 日清オイリオグループ株式会社
Priority date: 2015-11-27
Filing date: 2016-11-04
Publication date: 2017-06-01
Also published as: JP6674766B2; JP2017093404A

Abstract

Provided is a means for improving the performance of healthy horses. The means comprises feeding horses with an edible fat or oil that contains a triacylglycerol comprising, as a constituting fatty acid, a medium-chain fatty acid having 6-12 carbon atoms.

Description

Horse feed additive

The present invention relates to an equine feed additive. More particularly, the present invention relates to a horse feed additive capable of enhancing clinical laboratory test values for horse red blood cells and a horse feed using the feed additive.

Race horses and other horses have been improved to improve their performance such as competitive ability. On the other hand, in recent years, the importance of feeding management as well as breed improvement has been increasing. For example, various equine feed additives and equine feeds for the purpose of improving the health and performance of horses have been developed (Patent Documents 1 to 4).
By the way, triacylglycerol containing medium chain fatty acid as a constituent fatty acid is a nutrient which is known to be contained in the milk fat of various mammals, and has been used since 1960's in the field of human clinical practice. . For example, it is known to use the above-mentioned triacylglycerol to reduce body fat mass (Patent Document 5).

Patent No. 4702678 Patent No. 3232384 Patent No. 4115338 gazette Patent No. 5490355 gazette WO 2003/007932

Patent Document 5 describes the use of triacylglycerol to reduce body fat mass in animals such as humans. However, Patent Document 5 does not describe the use of the triacylglycerol for the purpose of improving the performance of a non-diseased horse (healthy horse).

The inventors of the present invention have intensively studied the use of triacylglycerol having medium-chain fatty acid as a constituent fatty acid for improving the performance of healthy horses. As a result, triacylglycerol having a specific kind of medium-chain fatty acid as constituent fatty acid Have been found to enhance clinical laboratory data on erythrocytes of horses not afflicted with The present invention has been made based on this finding.

That is, the present invention relates to the following aspects.

1. An equine feed additive containing an edible oil and fat, wherein the edible oil and fat contains triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid.

2. The equine feed additive according to the above 1, wherein the constituent fatty acid of the triacylglycerol having the medium chain fatty acid is composed only of the medium chain fatty acid having 6 to 12 carbon atoms.

3. The equine feed additive according to 1 or 2, wherein the constituent fatty acid of the triacylglycerol having the medium chain fatty acid is composed of only a medium chain fatty acid having 8 and / or 10 carbon atoms.

4. The equine feed additive according to any one of 1 to 3, wherein the content of the triacylglycerol having the medium-chain fatty acid is 70% by mass or more based on the total triacylglycerol amount of the edible fat and oil .

5. The equine feed additive as described in any one of 1 to 4 above, for enhancing clinical laboratory values of erythrocytes.

6. The equine feed additive according to any one of the above 1 to 5, wherein the clinical laboratory value of the red blood cells is any one of the red blood cell count, the amount of hemoglobin, and the hematocrit value.

7. A clinical laboratory value enhancer for equine erythrocytes, which comprises edible fat and oil, wherein the edible fat and oil contains triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid.

8. 7. The enhancer according to 7 above, wherein the clinical test value for red blood cells is any of red blood cell count, hemoglobin amount or hematocrit value.

9. 7. The enhancer according to 7 or 8 above, wherein the constituent fatty acid of triacylglycerol having medium chain fatty acid is composed of only medium chain fatty acid having 6 to 12 carbon atoms.

10. The enhancer according to any one of 7 to 9, wherein the constituent fatty acid of the triacylglycerol having the medium chain fatty acid is composed of only a medium chain fatty acid having 8 and / or 10 carbon atoms.

11. The enhancer according to any one of 7 to 10, wherein the content of the triacylglycerol having the medium chain fatty acid is 70% by mass or more with respect to the total amount of triacylglycerols of the edible oil and fat.

12. A horse performance improver comprising the clinical laboratory value enhancer for horse red blood cells according to any one of 7 to 11 above.

13. The equine feed additive according to any one of 1 to 6, the enhancer of clinical laboratory test values for equine erythrocytes according to any one of 7 to 11, or the equine according to 12 Horse feed containing performance enhancers.

14. The equine feed additive according to any one of 1 to 6, the enhancer of clinical laboratory test values for equine erythrocytes according to any one of 7 to 11, or the equine according to 12 The equine feed according to the above 13, wherein the performance enhancer is contained in an amount corresponding to 1.0 to 17.5% of the daily calorie intake of the horse.

15. The equine feed containing the equine feed additive according to any one of 1 to 6 above, the enhancer of clinical test values for equine red blood cells according to any one of 7 to 11, or 12 A method for rearing a horse comprising the step of feeding the horse performance improver according to any one of the above or 13 or 14 to the horse.

As shown in the following examples, according to the present invention, it is possible to enhance clinical laboratory data on erythrocytes of horses not affected by the disease.
Thus, the present invention can provide a means to improve the performance of healthy horses by feeding management without resorting to breeding.

FIG. 1 is a diagram showing the rate of change of “red blood cell count”, “hemoglobin amount” and “hematocrit value” by intake of LCT or MCT, with 100% before intake.

[Horse feed additive]
One embodiment of the present invention is a horse feed additive comprising an edible oil containing triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid. Hereinafter, the equine feed additive will be described.

[Constituted fatty acid]
The equine feed additive of the present invention comprises an edible oil and fat containing triacylglycerol (ie, oil and fat) having medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid as an active ingredient. The carbon number of the medium chain fatty acid is more preferably a combination of 8 to 12, more preferably 8 and / or 10. The triacylglycerol may be a triacylglycerol consisting only of medium-chain fatty acids such as those having 6 to 12 carbon atoms, more preferably 8 to 12 carbon atoms, and even more preferably 8 and / or 10 carbon atoms. Furthermore, it may be a mixed acid triacylglycerol containing medium-chain fatty acids having 6 to 12 carbon atoms and other fatty acids as constituent fatty acids. Here, the bonding position of each medium-chain fatty acid to glycerin is not particularly limited. In the case of mixed triacylglycerol, part of the constituent fatty acids may contain fatty acids other than 6 to 12 carbon atoms, and for example, long chain fatty acids having 14 or more carbon atoms may be contained. Good.
In addition, the triacylglycerol having medium-chain fatty acid used in the present invention may be, for example, a mixture of fats and oils of a plurality of different molecular species, such as a mixture of trioctanoyl glycerol and tridecanoyl glycerol. It does not matter whether it is in the form of liquid, solid or powder.
Here, the medium-chain fatty acid having 6 to 12 carbon atoms is preferably a linear saturated fatty acid.

The triacylglycerol having a medium-chain fatty acid that can be used in the present invention is preferably a triacylglycerol consisting of only a medium-chain fatty acid having 6 to 12 carbon atoms (hereinafter also referred to as "MCT"), in particular. More preferred is a triacylglycerol in which the fatty acid consists of only a medium-chain fatty acid having 8 and / or 10 carbon atoms.
Triacylglycerols having medium-chain fatty acids which can be used in the present invention can be produced by conventionally known methods. For example, fatty acids having 6 to 12 carbon atoms and glycerol are heated at 50 to 250 ° C., more preferably 120 to 180 ° C., under catalyst, preferably without catalyst, and preferably under reduced pressure, for dehydration condensation. It can be manufactured by
Here, the catalyst is not particularly limited, and, for example, an acid catalyst or a base catalyst used for ordinary transesterification can be used. Under reduced pressure is, for example, 0.01 to 100 Pa, preferably 0.05 to 75 Pa, and more preferably 0.1 to 50 Pa. At this time, the water content in the system is preferably small, and more preferably 0.2% by weight or less.

Edible fats and oils in the present invention refer to fats and oils that can be fed to horses. The edible fat and oil may be any one containing triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms described above as the active ingredient, and in addition, feeding to horses is possible, and Other fats and oils may be contained as long as the effects of the present invention are not impaired. Other fats and oils include common edible oils such as soybean oil, rapeseed oil, corn oil, sesame oil, sesame salad oil, perilla oil, linseed oil, peanut oil, safflower oil, high oleic safflower oil, sunflower oil, high oleic acid Sunflower oil, cottonseed oil, grape seed oil, macadamia nut oil, hazelnut oil, pumpkin seed oil, walnut oil, soy sauce, tea seed oil, sesame oil, borage oil, olive oil, rice bran oil, wheat germ oil, palm oil, palm kernel oil, Palm oil, cocoa butter, beef tallow, lard, chicken fat, milk fat, fish oil, seal oil, algal oil, these fats and oils that have been desaturated by breed improvement, hydrogenated fats and oils of these, fractionated fats and the like can be mentioned.

The amount (content) of triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms contained in the edible oil or fat is preferably 70% by mass or more, more preferably the total amount of triacylglycerol in the edible oil or fat Is 80% by mass or more, more preferably 90% by mass or more, and particularly preferably 95% by mass or more. When the content is 70% by mass or more, higher blending effects can be obtained. In addition, the upper limit of content is 100 mass%.

The horse feed additive of the present invention does not impair the effects of the present invention, and other components such as dextrin, excipients such as starch, emulsifiers, vitamins, minerals etc. It may be one containing an agent or a nutritional supplement or the like added to a known horse feed. The additive may be in any form such as liquid, solid or powder.

The “triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid”, which is an active ingredient of the equine feed additive of the present invention, can enhance the clinical laboratory value of equine erythrocytes.
Laboratory test values for horse red blood cells include red blood cell count, hemoglobin amount, hematocrit value and red blood cell constant (MCV, MCH, MCHC). Among these, the present invention can be suitably used to enhance the number of red blood cells, the amount of hemoglobin, and the hematocrit value.
Clinical laboratory data on equine erythrocytes can be measured using a test method generally used for clinical blood tests in the veterinary field.

[Enhancers for clinical laboratory test values for equine erythrocytes]
As mentioned above, "triacylglycerol having medium-chain fatty acid having 6 to 12 carbon atoms as constituent fatty acid" which can be used as an active ingredient of the equine feed additive of the present invention enhances the clinical test value of equine erythrocytes. be able to.
Therefore, one aspect of the present invention is a clinical laboratory value enhancer for equine erythrocytes, which comprises an edible oil containing triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid.
The definitions of “triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as constituent fatty acid” and “food oil and fat”, which are active ingredients of the enhancer, are as described above for the horse feed additive.

Horse Performance Improver
The aforementioned "laboratory value of horse red blood cells" is an indicator of the state of red blood cells having the function of delivering oxygen from the lungs to each tissue. An increase in these values means the improvement of horse's resistance to exercise and endurance, and thus the improvement of horse's performance.
Therefore, one aspect of the present invention is a horse performance improver comprising the aforementioned "enhancement agent for clinical laboratory test value for horse red blood cells".
"Horse performance" refers to any ability of the horse that involves red blood cell condition, such as competitive ability for race horses, transportability for farm horses, etc.
The definitions of “triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as constituent fatty acid” and “food oil and fat”, which are active ingredients of the performance improver, are as described above for the horse feed additive.

[Horse feed]
The "horse feed additive" of the present invention can be added to rough feed to make a horse feed.
The “contest value enhancer for horse red blood cells” and “horse performance enhancer” of the present invention may be fed to horses alone, but when used as a horse feed in combination with rough feed, body conditioning It is preferable because it can further improve the score (BCS), the condition of hair, the condition of feces, the condition after training, the amount of feeding, the intake condition and the like.
Therefore, one aspect of the present invention is a horse feed comprising the above-mentioned "horse feed additive", "horseradish red blood cell clinical value enhancer" or "horse performance enhancer".
As forage, those generally used for equine feed can be used without particular limitation. In general, the forage includes grasses (Timothy, alfalfa, heycube, orchardgrass, Italian ryegrass, etc.).
The horse feed according to the present invention may further contain grains (eg, barley, oats, corn, etc.), vitamins, and optional components such as minerals, if necessary.
The amount (content) of the above-mentioned "horse feed additive", "a laboratory test value increaser for horse red blood cells" or "a horse performance improver" contained in the horse feed is the daily amount of the horse Preferably, the amount corresponds to preferably 1.0 to 17.5%, more preferably 3.0 to 15.0%, still more preferably 5.0 to 12.5% of the calories ingested. When the content is 1.0 to 17.5%, a higher blending effect can be obtained.
The calories of the "horse feed additive", the "enhancement agent of clinical laboratory test value for horse red blood cells" or the "horse performance improver" can be calculated according to a generally known method. For example, a modified atwater method, a cylinder calorimeter method, a calorie answer method and the like can be mentioned.
The equine feed of the present invention is produced by adding the above-mentioned "horse feed additive", "horseradish red blood cell clinical value enhancer" or "horse performance enhancer" to roughage and mixing. be able to.

[How to breed a horse]
A further aspect of the present invention is a method of rearing a horse, comprising the steps of: feeding a horse feed, an enhancer, a performance enhancer, or a horse feed containing the above-mentioned feed additive to a horse.
There is no particular restriction on the feeding means, and it may be a general feeding means such as feeding the feed in a manger and feeding it to horses, but the above-mentioned "horse feed additive", "clinical examination on horse red blood cells" Preferably, the value of the value enhancer or the horse performance enhancer is provided at 1.0 to 17.5% of the daily calorie intake of the horse.

Horses to be fed are not particularly limited as long as they are non-diseased horses (healthy horses). Here, disease refers to a condition (e.g., anemia) in which laboratory values for red blood cells are lower than the corresponding laboratory values in healthy horses. The veterinarian determines if there is disease. One of the important features of the present invention is the ability to significantly enhance the clinical laboratory values for red blood cells of horses that are already in a healthy condition.
Although the type of horse to be fed is not limited, the present invention can be suitably used for racehorses (especially light stallions).
In addition, the age of the feed subject horse is not limited, and the present invention can be applied to the entire life stage of the horse. In the case of racehorses, after being born on a production ranch, they are trained and trained on a breeding ranch, and then they live on racehorses at a training center, but the present invention relates to breeding horses that are bred on a breeding ranch (generally 1 It can be suitably used for years old and 2 and a half years old.

Next, the effects of the present invention will be specifically described by way of examples, but the present invention is not limited to the examples.

In the following, “%” indicates mass% unless otherwise specified.
The analysis of the triacylglycerol composition of fats and oils was performed using a gas chromatograph method (according to JAOCS, vol 70, 11, 1111-1114 (1993)).
Analysis of constituent fatty acids of fats and oils was performed using gas chromatography (based on AOCS Ce1f-96).

<Edible fat and oil>
[MCT]: As "the edible oil and fat containing triacylglycerol having medium-chain fatty acid having 6 to 12 carbon atoms as constituent fatty acid" of the present invention, fatty acid constituting triacylglycerol comprises octanoic acid (having 8 carbon atoms) and Decanoic acid (carbon number 10), consisting of only medium-chain fatty acids having 8 and 10 carbon atoms, in which the proportion (mass ratio) of fatty acids constituting triacylglycerol is octanoic acid: decanoic acid = 75: 25 Triacylglycerol (manufactured by Nisshin Oillio Group, Inc., trade name: MCT-ODO) (hereinafter, also referred to as "MCT") was used.
The amount (content) of triacylglycerol having a medium-chain fatty acid having 8 or 10 carbon atoms in the above MCT was 100% by mass with respect to the total amount of triacylglycerol of the MCT.
[LCT]: fatty acid constituting triacylglycerol is palmitic acid (carbon number 16), oleic acid (carbon number 18), linoleic acid (carbon number 18) and others, and the proportion of fatty acid constituting triacylglycerol (Mass ratio) is palmitic acid: oleic acid: linoleic acid: other = about 4: 61: 20: 15 Canola oil (manufactured by Nisshin Oillio Group, Inc., trade name: Nisshin Canola Oil) (hereinafter, " (Also called LCT) was used.
LCT is triacylglycerol having no medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid, and corresponds to a comparative example.

<Feeding method>
Two-year-old breeders determined not to be ill at a racehorse breeding ranch, such as oats, brans, corns, rice bran, etc., with rough feed such as common fodder (eg grass (Timoshi etc.)) A diet prepared by mixing 200 grams of edible fats and oils with a concentrated feed and a feed appropriately mixed with trace nutrient components such as vitamins and minerals was fed for 3 weeks and tested.
The group (LCT group: 10 stallions and 10 mares) using LCT as edible fat and oil corresponds to the comparative example, and the group using MCT (MCT group: 10 stallions and 10 mares) corresponds to the example .
In each group, the amount (200 g) of the edible fat and oil added to the horse feed was an amount corresponding to 7% of the daily calorie intake of the horse. Calorie calculation was measured by the modified atwater method.
The test period was 3 weeks, and blood was collected before the start of the test and after the test to conduct various clinical tests.
Statistical analysis of blood laboratory test results was performed by repeated measurement (Repeated-Measures ANOVA). If significant intra-group variation is observed, continue paired t-test, and if interaction is observed, perform multiple comparisons using the Turkey-kramer method. (P <0.05). The clinical test results before and after the test in each group are shown in Table 1 below.

In the clinical examination items in Table 1, although both total protein and albumin are indicators of the nutritional status of the horse, no significant difference was observed between the LCT group and the MCT group before and after the test. This indicates that MCT has no adverse effect on the nutritional status of the horse and indicates that the condition of the horse is healthy (no change between the groups).
On the other hand, in the "red blood cell count", "hemoglobin amount" and "hematocrit value", which are clinical test items related to red blood cells, the MCT group improves significantly higher than the LCT group at the change rate when the start of the test is 100%. Is shown (Figure 1).
The results of this study demonstrate that MCT has enhanced clinical laboratory values for horse red blood cells. And in healthy horses, the ability to increase the laboratory test values for these red blood cells, MCT, to improve the resistance to endurance and endurance of the horse, and thus to improve the performance of the horse It is easy to understand that you can contribute.

The invention can be used in the field of industry involving horses.

Claims

An equine feed additive containing an edible oil and fat, wherein the edible oil and fat contains triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid.
The equine feed additive according to claim 1, wherein the constituent fatty acid of triacylglycerol having the medium chain fatty acid is composed only of the medium chain fatty acid having 6 to 12 carbon atoms.
The equine feed additive according to claim 1 or 2, wherein the constituent fatty acid of triacylglycerol having the medium chain fatty acid is composed only of the medium chain fatty acid having 8 and / or 10 carbon atoms.
The equine feed additive according to any one of claims 1 to 3, wherein the content of the triacylglycerol having the medium chain fatty acid is 70% by mass or more based on the total triacylglycerol amount of the edible fat and oil. Agent.
The equine feed additive according to any one of claims 1 to 4, for enhancing clinical laboratory values of erythrocytes.
The equine feed additive according to any one of claims 1 to 5, wherein the clinical laboratory value of the red blood cells is any one of the red blood cell count, the hemoglobin amount and the hematocrit value.
A clinical laboratory value enhancer for equine erythrocytes, which comprises edible fat and oil, wherein the edible fat and oil contains triacylglycerol having a medium-chain fatty acid having 6 to 12 carbon atoms as a constituent fatty acid.
The enhancing agent according to claim 7, wherein the clinical test value for red blood cells is any one of red blood cell count, hemoglobin amount or hematocrit value.
The enhancer according to claim 7 or 8, wherein the constituent fatty acid of triacylglycerol having a medium chain fatty acid is composed only of a medium chain fatty acid having 6 to 12 carbon atoms.
The enhancer according to any one of claims 7 to 9, wherein the constituent fatty acid of the triacylglycerol having the medium chain fatty acid is composed only of a medium chain fatty acid having 8 and / or 10 carbon atoms.
The enhancer according to any one of claims 7 to 10, wherein the content of the triacylglycerol having a medium-chain fatty acid is 70% by mass or more based on the total triacylglycerol amount of the edible fat and oil.
A horse performance improver comprising the clinical laboratory value enhancer for horse red blood cells according to any one of claims 7 to 11.
The equine feed additive according to any one of claims 1 to 6, the enhancer of clinical laboratory test values for equine red blood cells according to any one of claims 7 to 11, or claim 12 Horse feed, including horse performance enhancers.
The equine feed additive according to any one of claims 1 to 6, the enhancer of clinical laboratory test values for equine red blood cells according to any one of claims 7 to 11, or claim 12 The horse feed according to claim 13, wherein the horse performance improver is contained in an amount corresponding to 1.0 to 17.5% of the daily calorie intake of the horse.
An equine feed comprising the equine feed additive according to any one of claims 1 to 6, an enhancer of clinical laboratory test values for equine red blood cells according to any one of claims 7 to 11, A method of breeding a horse comprising the step of feeding the horse a horse performance improver according to claim 12, or a horse feed according to claim 13 or 14.