WO2017087698A1 - Insert d'incubateur pour expérimentation sur culture automatisée - Google Patents

Insert d'incubateur pour expérimentation sur culture automatisée Download PDF

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Publication number
WO2017087698A1
WO2017087698A1 PCT/US2016/062573 US2016062573W WO2017087698A1 WO 2017087698 A1 WO2017087698 A1 WO 2017087698A1 US 2016062573 W US2016062573 W US 2016062573W WO 2017087698 A1 WO2017087698 A1 WO 2017087698A1
Authority
WO
WIPO (PCT)
Prior art keywords
incubator
millimeters
module
microscope
support platform
Prior art date
Application number
PCT/US2016/062573
Other languages
English (en)
Inventor
Donald E. Ingber
Richard Novak
Miles INGRAM
Susan CLAUSON
Toni DIVIC
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2017087698A1 publication Critical patent/WO2017087698A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)

Definitions

  • FIG. 11 A is a display screen with flow rate settings for a pump user interface.
  • FIG. 11D is a display screen with flush interval settings for the pump user interface of FIG. 11 A.
  • FIG. 12A is an isometric view of a fluidics plate assembly, according to another embodiment.
  • the incubator insert system 100 includes a support platform 104 that is configured to fit within the internal incubator space (defined generally by the length CL, the height CH, and the width CW).
  • the support platform 104 has a generally rectangular support base 106 with a base length BL and a base width BW that are each sized in accordance with the respective size and shape of the incubator.
  • the base length BL is less than about 607 mm and the base width BW is less than about 757 mm.
  • the support platform 104 further has a support frame 108 that extends upward along a back edge of the support base 106.
  • the support frame 104 has a frame height FH, which according to one example is less than about 670 mm.
  • the robotic pipettor module 110 includes one or more features, including, by way of example, a 3-axis positioning of a commercial pipette head 112 (e.g., a Incontinent pipettor or a Z series pipettor), with approximately 300 mm by 300 mm range of lateral motion along each lateral axis X and Y, and approximately 150 mm vertical range of motion along a vertical axis Z.
  • a commercial pipette head 112 e.g., a Incontinent pipettor or a Z series pipettor
  • the robotic pipettor module 110 includes position and acceleration control through GRBL 3-axis shield, belt driven X and Y axes for speed, and/or a screw-driven Z-axis for added precision.
  • the microscope module 116 includes light- emitting diodes ("LEDs") used for excitation, and/or external LEDs mounted above chips for brightfield imaging.
  • the microscope module is controlled and automated through a platform interface, and/or includes periodic image-based analysis.
  • the chip holder module 118 includes one or more features, including, by way of example, a holder for up to 12 standard microscope slides (25 x 75 mm), or a holder for 24 inlet and 24 outlet reservoirs, e.g., standard syringe bodies and custom reservoirs.
  • the incubator insert system 100 is configured to accommodate any number of culture compartments/containers, e.g., the range of culture compartments/containers includes a range of 1 to 12, a range from 10 to 100, or a range that exceeds 100. The range is based on the dimensions of the compartments/containers.
  • the incubator insert system 100 is used for automated recovery of, for example, tissue or embryos, using pipettor or another attachment, which could be used for DNA/RNA analysis or transfer for external growth of organisms or cells.
  • workflow and/or protocol changes are minimized in experiments by superimposing automation on existing microfluidic or other culture systems rather than necessitating special disposables.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un système d'insert d'incubateur qui comprend une plate-forme de support conçue pour s'adapter à l'intérieur d'un incubateur défini par une longueur d'incubateur, une hauteur d'incubateur et une largeur incubateur. Le système comprend une pipette robotisée fixée à la plate-forme de support servant à introduire un fluide dans un ou plusieurs récipients de fluide, un système de perfusion fixé à la plate-forme de support et en communication fluidique avec la pipette robotisée, et un module de microscope fixé à la plate-forme de support et conçu pour capturer des images des échantillons sur la plate-forme de support.
PCT/US2016/062573 2015-11-18 2016-11-17 Insert d'incubateur pour expérimentation sur culture automatisée WO2017087698A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562257043P 2015-11-18 2015-11-18
US62/257,043 2015-11-18
US201662368804P 2016-07-29 2016-07-29
US62/368,804 2016-07-29

Publications (1)

Publication Number Publication Date
WO2017087698A1 true WO2017087698A1 (fr) 2017-05-26

Family

ID=58717837

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/062573 WO2017087698A1 (fr) 2015-11-18 2016-11-17 Insert d'incubateur pour expérimentation sur culture automatisée

Country Status (1)

Country Link
WO (1) WO2017087698A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019046613A1 (fr) * 2017-08-30 2019-03-07 Microbedx, Inc. Dispositif pour l'optimisation de la croissance de micro-organismes en culture liquide

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5009185A (en) * 1989-05-09 1991-04-23 Wescor, Inc. Apparatus for applying a controlled amount of reagent to a microscope slide or the like
US5380662A (en) * 1990-06-01 1995-01-10 Robbins Scientific Corporation Hybridization incubator with rotisserie mechanism
US6099461A (en) * 1997-10-28 2000-08-08 Kendro Laboratory Products Gmbh Device for humidifying the useful space of a climatic test cabinet
US20030113232A1 (en) * 2001-12-14 2003-06-19 Agilent Technologies, Inc. Tri-axial handling apparatus
US6602701B2 (en) * 2000-01-11 2003-08-05 The General Hospital Corporation Three-dimensional cell growth assay
US6847481B1 (en) * 2001-10-26 2005-01-25 Ludl Electronics Products, Ltd. Automated slide loader cassette for microscope
US20060210451A1 (en) * 2001-08-16 2006-09-21 Anderson Clifford L Fixtures for use in parallel processing bio-chips
WO2006098441A1 (fr) * 2005-03-17 2006-09-21 Hamamatsu Photonics K.K. Cassette a verres de preparation microscopique et etui a cassette
US20060210435A1 (en) * 2005-03-07 2006-09-21 Tino Alavie Automated analyzer
US20110015896A1 (en) * 2009-07-16 2011-01-20 Paul John Gendron Accurate and computationally efficent method of non-central chi square probability density function
US20110030803A1 (en) * 2009-08-04 2011-02-10 Roche Diagnostics Operations, Inc. Sample Preparation Dosing Unit
US20130023041A1 (en) * 1996-11-01 2013-01-24 Greenberger Joel S Method and Apparatus for Holding Cells
US20140073043A1 (en) * 2011-09-25 2014-03-13 Theranos, Inc. Systems and methods for multi-analysis
US20140071452A1 (en) * 2012-09-10 2014-03-13 The Trustees Of Princeton University Fluid channels for computational imaging in optofluidic microscopes

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5009185A (en) * 1989-05-09 1991-04-23 Wescor, Inc. Apparatus for applying a controlled amount of reagent to a microscope slide or the like
US5380662A (en) * 1990-06-01 1995-01-10 Robbins Scientific Corporation Hybridization incubator with rotisserie mechanism
US20130023041A1 (en) * 1996-11-01 2013-01-24 Greenberger Joel S Method and Apparatus for Holding Cells
US6099461A (en) * 1997-10-28 2000-08-08 Kendro Laboratory Products Gmbh Device for humidifying the useful space of a climatic test cabinet
US6602701B2 (en) * 2000-01-11 2003-08-05 The General Hospital Corporation Three-dimensional cell growth assay
US20060210451A1 (en) * 2001-08-16 2006-09-21 Anderson Clifford L Fixtures for use in parallel processing bio-chips
US6847481B1 (en) * 2001-10-26 2005-01-25 Ludl Electronics Products, Ltd. Automated slide loader cassette for microscope
US20030113232A1 (en) * 2001-12-14 2003-06-19 Agilent Technologies, Inc. Tri-axial handling apparatus
US20060210435A1 (en) * 2005-03-07 2006-09-21 Tino Alavie Automated analyzer
WO2006098441A1 (fr) * 2005-03-17 2006-09-21 Hamamatsu Photonics K.K. Cassette a verres de preparation microscopique et etui a cassette
US20110015896A1 (en) * 2009-07-16 2011-01-20 Paul John Gendron Accurate and computationally efficent method of non-central chi square probability density function
US20110030803A1 (en) * 2009-08-04 2011-02-10 Roche Diagnostics Operations, Inc. Sample Preparation Dosing Unit
US20140073043A1 (en) * 2011-09-25 2014-03-13 Theranos, Inc. Systems and methods for multi-analysis
US20140071452A1 (en) * 2012-09-10 2014-03-13 The Trustees Of Princeton University Fluid channels for computational imaging in optofluidic microscopes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019046613A1 (fr) * 2017-08-30 2019-03-07 Microbedx, Inc. Dispositif pour l'optimisation de la croissance de micro-organismes en culture liquide

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