WO2017067054A1

WO2017067054A1 - Sterilization tray with magnetic base for superclean bench

Info

Publication number: WO2017067054A1
Application number: PCT/CN2015/098605
Authority: WO
Inventors: 邱一华; 彭聿平; 高江宁; 刘展; 曹蓓蓓
Original assignee: 南通大学
Priority date: 2015-10-20
Filing date: 2015-12-24
Publication date: 2017-04-27
Also published as: CN105233899A; ZA201703623B

Abstract

A sterilization tray with a magnetic base for a super clean bench, comprising a magnetic base (6) that generates a magnetic attraction force, wherein the side surface of the magnetic base (6) is a magnetic base attraction surface (4) for attracting the magnetic base (6) on an iron vertical edge plate in a superclean bench; the bottom surface (7) of the magnetic base is in contact with the bottom surface in the superclean bench; the upper surface of the magnetic base (6) is fixedly provided with an upright column pedestal (3); a column (1) is provided on the upright column pedestal (3); a horizontal member height positioning lock is installed on the column (1); a horizontal sterilization tray member that is sleeved on the column (1) and can horizontally rotate on the column (1) is provided above the horizontal member height positioning lock; the horizontal sterilization tray member comprises a connecting sleeve (18) that is connected, by means of a horizontal arm (15), to a horizontal culture plate placing plate (16) used for placing a culture plate; an anti-sliding frame (17) is provided around the horizontal culture plate placing plate (16).

Description

Ultra-clean table with magnetic base sterilization tray

Technical field

[0001] The present invention relates to a magnetic base sterilization tray for a clean bench.

Background technique

[0002] In vitro culture experiments of cells are a kind of scientific research method commonly used in scientific research experiments. In the cell culture project, in a sterile environment provided by the clean bench, a cell culture plate (hereinafter referred to as a culture plate) of a sterile plastic material is used as a container, and the cultured cells are added to the test liquid, and the test liquid is replaced. Carry out experimental operations such as incubation and cultivation of cells.

[0003] In the above cell culture experiment, it is necessary to perform aseptic treatment on a culture plate, in particular, a plastic culture plate of a secondary use. Since the culture plates cannot be autoclaved, the general laboratory does not have the conditions for radiation sterilization. Therefore, it is common to sterilize the culture plate by ultraviolet light irradiation in a clean bench under aseptic conditions. In order to ensure the effect of UV light irradiation, the distance between the UV lamp and the culture plate should be controlled within 30 cm. Therefore, it is necessary to find some objects in the clean bench to raise the plate so that it is close to the source of the UV lamp. Since the ultra-clean platform has certain requirements for the placed items, it is rather troublesome to find these high-level objects, and there is also a problem that the height of the mat is unstable and the culture board is dropped.

technical problem

[0004] The object of the present invention is to provide a magnetic base sterilization tray for a clean platform with reasonable structure, convenient use and adjustable space.

Problem solution

Technical solution

The technical solution of the present invention is:

[0006] A magnetic base sterilization tray for a clean bench, comprising: a magnetic seat for generating an adsorption magnetic force, wherein a side of the magnetic seat is a magnetic body for adsorbing the magnetic base on a vertical side plate of the iron in the ultra-clean platform; The suction surface of the seat, the bottom surface of the magnetic seat is in contact with the bottom surface of the clean bench, the upper surface of the magnetic seat is fixed with the column bottom plate, the column is provided on the column bottom plate; the horizontal member height positioning lock is mounted on the column, and the horizontal member height positioning lock is arranged above the column. a sterilizing tray horizontal member that can be horizontally rotated on the column; the sterilizing tray horizontal structure The piece includes a connecting sleeve for fitting the horizontal member of the sterilization tray on the column of the magnetic base, and helps the horizontal member of the sterilization tray to move at a large angle in the horizontal direction, and the connecting sleeve is cultured by the horizontal arm and the horizontal The plate of the plate is placed horizontally, and the horizontal plate of the plate is provided with a non-slip frame to prevent the plate from slipping off.

[0007] The horizontal member height positioning lock comprises a positioning lock body for forming a positioning cylindrical space of the positioning lock and the magnetic base column; the positioning lock body is composed of two left and right pieces, and the left and right two pieces are passed through the positioning lock hinge The connection enables the huddle cylindrical space of the positioning lock to be effectively spliced; the positioning lock fastening screw is arranged on the left and right side of the positioning lock body, and the positioning lock fastening screw is tightened to make the positioning lock tightly entangled in the magnetic On the column of the base, loosen the positioning lock fastening screw, remove the positioning lock to the magnetic base column, and move the position of the positioning lock up and down.

[0008] A positioning lock silicone pad is arranged in the positioning lock body, and the positioning lock silicone pad and the magnetic base column are engaged, so that the positioning lock is fixed at a height position required by the magnetic base column.

[0009] The magnetic seat includes a soft magnetic material, and a soft magnetic material is provided with a non-magnetic material that separates the soft magnetic material into uniform upper and lower halves, and a constant magnet is disposed in a cylindrical space surrounded by a soft magnetic material. The constant magnet and the constant magnet are connected to each other. When the constant magnet rotates to the off position, the N and S poles of the constant magnet are in a horizontal position, and the magnetic lines of force are separated by two soft magnetic materials separated by a non-magnetic material. In the closed circuit, the magnetic seat adsorption surface is not magnetic and can move freely; when the constant magnet rotates to the 幵 position, the N and S poles of the constant magnet are in the up and down position, and the N and S poles of the constant magnet are respectively aligned. The two soft magnetic materials of the magnetically permeable material are separated from the N pole to the soft magnetic material to the iron vertical side plate in the ultra clean table to another soft magnetic material to the S pole, forming a magnetic line close, and adsorbing the magnetic seat adsorption surface On the iron vertical side panel in the clean bench.

Advantageous effects of the invention

Beneficial effect

[0010] The present invention can eliminate the trouble of looking for a high height in the ultra clean bench every time the illuminating plate is irradiated with an ultraviolet lamp. The tray is provided with a slip-off frame around it to prevent the culture plate from slipping off. Moreover, the upper and lower spatial positions of the sterilization tray can be changed by adjusting the position of the horizontal member height positioning lock on the column. It is also possible to change the horizontal spatial position of the sterilization tray by adjusting the horizontal angle of the horizontal member of the sterilization tray. It can be seen that the magnetic base sterilization tray for the ultra-clean platform of this application is a tray device which can be used in the ultra-clean platform, which has adjustable storage space, reasonable structure and convenient use for ultraviolet irradiation of the culture plate. Brief description of the drawing

DRAWINGS

[0011] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

1 is a front view of a magnetic base.

2 is a longitudinal cross-sectional view of a magnetic seat.

[0014] FIG. 3 is a top plan view of the horizontal member height positioning lock.

4 is a plan view of a horizontal member of a sterilization tray.

[0016] In the figure:

[0017] 1. Column: vertically placed in the center of the upper surface of the magnetic seat, connected to the horizontal member of the sterilization tray. The height of the column is 25-35cm (for example, 25cm, 30cm. 35cm), and the diameter of the column is 2-3cm (for example, 2cm, 2.5cm, 3cm).

[0018] 2. Chassis fixing screw: Fix the column chassis to the upper surface of the magnetic base.

[0019] 3. Column chassis: connected to the bottom end of the column, forming a fixed surface of the bottom end of the column and the upper surface of the magnetic seat.

[0020] 4. Magnetic seat adsorption surface: The magnetic base is adsorbed on the vertical side plate of the iron in the clean bench.

[0021] 5. Constant magnet rotation is turned off: The constant magnet is rotated to the off position, the magnetic force of the adsorption surface of the magnetic base disappears, and the magnetic base can be freely moved; the constant magnet is rotated to the position of the crucible, and the magnetic base adsorption surface generates a magnetic force. The magnetic base is firmly attached to the vertical side panel of the iron in the clean bench.

[0022] 6. Magnetic seat: The adsorption magnetic force is generated.

[0023] 7. The bottom of the magnetic base: contact with the bottom surface of the clean bench to increase the stability of the magnetic base.

8. Constant Magnet: A magnetic generating source.

[0025] 9. Non-magnetic material: The soft magnetic material of the magnetic seat is divided into uniform upper and lower halves.

[0026] 10. Soft magnetic material: According to the position of the constant magnet, the magnetic seat is separately magnetized, or demagnetized.

[0027] 11. Positioning lock silicone pad: Cooperating with the magnetic base column to form a large static friction force, so that the positioning lock is well fixed at the height position required by the magnetic base column.

[0028] 12. Positioning lock fastening screw: Tightening the screw allows the positioning lock to be tightly held on the column of the magnetic base

Loosen the screw, remove the positioning lock to the magnetic base column, and move the positioning lock up and down.

[0029] 13. Positioning lock body: forming a positioning cylindrical lock and a magnetic base column. [0030] 14. Positioning lock hinge: enables the cohesive cylindrical space of the positioning lock to be effectively engaged.

[0031] 15. Horizontal arm: The connecting sleeve and the culture plate are horizontally placed.

[0032] 16. Plates Place the plates horizontally: Place the cell culture plates.

[0033] 17. Non-slip off frame: Prevent slippage of the culture plate.

[0034] 18. Connecting sleeve: The sterilizing tray horizontal member is placed on the column of the magnetic base, and the horizontal member of the sterilizing tray is horizontally moved (180° large angle movement).

BEST MODE FOR CARRYING OUT THE INVENTION

[0035] Example 1 :

[0036] A magnetic base sterilization tray for a clean bench includes a magnetic seat for generating an adsorption magnetic force, and a side of the magnetic seat is a magnetic seat adsorption surface for adsorbing the magnetic base on a vertical side plate of the iron in the clean bench. The bottom surface of the magnetic base is in contact with the bottom surface of the clean bench, the upper surface of the magnetic seat is fixed with the column bottom plate, and the column is provided on the column bottom plate; the horizontal member height positioning lock is mounted on the column, and the horizontal member height positioning lock is arranged above the column, and the a horizontally rotating sterilizing tray horizontal member on the column; the sterilizing tray horizontal member includes a connecting sleeve, the connecting sleeve is used for tying the sterilizing tray horizontal member on the column of the magnetic base, and assisting the sterilizing tray horizontal member In the horizontal direction of large-angle movement, the connecting sleeve is connected to the horizontally placed plate of the culture plate on which the culture plate is placed through the horizontal arm, and the anti-slip frame is arranged around the horizontally placed plate of the culture plate to prevent the culture plate from slipping off.

[0037] The height of the column is 25 cm, and the diameter of the column is 2 cm.

[0038] The horizontal member height positioning lock comprises a positioning lock body for forming a positioning cylindrical space of the positioning lock and the magnetic base column; the positioning lock body is composed of two left and right pieces, and the left and right two pieces are passed through the positioning lock hinge The connection enables the huddle cylindrical space of the positioning lock to be effectively spliced; the positioning lock fastening screw is arranged on the left and right side of the positioning lock body, and the positioning lock fastening screw is tightened to make the positioning lock tightly entangled in the magnetic On the column of the base, loosen the positioning lock fastening screw, remove the positioning lock to the magnetic base column, and move the position of the positioning lock up and down.

[0039] A positioning lock silicone pad is arranged in the positioning lock body, and the positioning lock silicone pad and the magnetic base column are engaged, so that the positioning lock is fixed at a height position required by the magnetic base column.

[0040] the magnetic seat includes a soft magnetic material, and the soft magnetic material is disposed to separate the soft magnetic material into uniform upper and lower portions Two non-magnetic materials, a constant magnet is arranged in the cylindrical space surrounded by the soft magnetic material, and the constant magnet is connected with the constant magnet. When the constant magnet rotates to the off position, the constant magnet N The S pole is in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials separated by the non-magnetic material, and the magnetic seat adsorption surface is free from magnetism and can move freely; when the constant magnet rotates to the position of the crucible, the constant The N and S poles of the magnet are in the up and down position, and the N and S poles of the constant magnet are respectively aligned with the two soft magnetic materials separated by the non-magnetic material, and the iron perpendicular from the N pole to the soft magnetic material to the ultra clean table The side plate to another soft magnetic material to the S pole forms a magnetic line close, and the magnetic seat adsorption surface is adsorbed on the iron vertical side plate in the ultra clean table.

[0041] Method of use of the invention:

[0042] Pretreatment: Wiping and sterilizing the components of the magnetic base sterilization tray with the disinfectant to ensure that the magnetic base sterilization tray installed in the clean bench does not change the aseptic environment in the clean bench (eg Frequent use, can be placed in the clean bench for a long time, can be wiped out with disinfectant disinfection).

[0043] Installation of the magnetic base: Turn the constant magnet to the off position (5). Thereafter, the N and S poles of the constant magnet 8 are in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials 10 which are separated by the non-magnetic conductive material 9, and the magnetic seat adsorption surface 4 is free from magnetism and is free to move. Place the magnetic base (composed of 1-7) in the desired position. The specific operation is as follows: The magnetic seat adsorption surface 4 is in contact with the vertical side plate of the iron in the clean bench, and the bottom surface 7 of the magnetic seat is in contact with the bottom surface in the clean bench. . The rotating constant magnet rotates to the position of the crucible, and the N and S poles of the constant magnet are in the upper and lower positions, and the N and S poles of the constant magnet are respectively aligned with the two soft magnetic materials separated by the non-magnetic material, from N From the soft magnetic material to the iron vertical side plate in the clean table to another soft magnetic material to the S pole, the magnetic field line is closed, and the magnetic seat adsorption surface can be firmly adsorbed on the iron vertical side in the ultra clean table. On the board.

[0044] Installation of the height positioning lock: Loosen the positioning lock fastening screw 12, put the cylindrical hole in the middle of the positioning lock (composed of 11-14) into the column 1, select the required height, and tighten the positioning lock fastening screw , the silicone pad 11 of the positioning lock is tightly engaged with the column.

[0045] Installation of the horizontal member of the sterilization tray and placement of the culture plate: taking the horizontal member of the sterilization tray (combined with 15-18), the connection sleeve 18 of the member is put into the column, and the lower edge of the connection sleeve is at the height Position the upper edge of the lock to complete the height positioning. The inner diameter of the connecting sleeve is slightly larger than the outer diameter of the column, so that the connecting sleeve can be freely rotated on the column without causing significant oscillation on the column. At this point, the installation of the magnetic base sterilization tray in the clean bench has been completed. Horizontal rotation culture according to experimental needs The plate is placed horizontally at the position of the plate 16, and the plate and the plate cover are placed in a space surrounded by the non-slip frame 17 (the inner faces of the culture holes and the plate cover face the ultraviolet lamp). They are sterilized by rubbing the UV lamp inside the clean bench. After the sterilization process is finished, turn off the UV lamp, cover the culture plate cover under aseptic conditions, and remove the sterilized culture plate from the sterilization tray for use.

[0046] Disassembly: Remove the magnetic base sterilization tray 超 in the clean bench, and move the sterilizing tray horizontal member vertically upwards to disengage the column, remove the sterilizing tray horizontal member, loosen the positioning lock fastening screw, and upward Move vertically, remove the height positioning lock from the column, and finally rotate the constant magnet to the closed position, and take out the magnetic base.

Example 2:

[0048] A magnetic base sterilization tray for a clean bench includes a magnetic seat for generating an adsorption magnetic force, and a side of the magnetic seat is a magnetic seat adsorption surface for adsorbing the magnetic base on a vertical side plate of the iron in the clean bench. The bottom surface of the magnetic base is in contact with the bottom surface of the clean bench, the upper surface of the magnetic seat is fixed with the column bottom plate, and the column is provided on the column bottom plate; the horizontal member height positioning lock is mounted on the column, and the horizontal member height positioning lock is arranged above the column, and the a horizontally rotating sterilizing tray horizontal member on the column; the sterilizing tray horizontal member includes a connecting sleeve, the connecting sleeve is used for tying the sterilizing tray horizontal member on the column of the magnetic base, and assisting the sterilizing tray horizontal member In the horizontal direction of large-angle movement, the connecting sleeve is connected to the horizontally placed plate of the culture plate on which the culture plate is placed through the horizontal arm, and the anti-slip frame is arranged around the horizontally placed plate of the culture plate to prevent the culture plate from slipping off.

[0049] The height of the column is 30 cm, and the diameter of the column is 2.5 cm.

[0050] The horizontal member height positioning lock comprises a positioning lock body for forming a positioning cylindrical space of the positioning lock and the magnetic base column; the positioning lock body is composed of two left and right pieces, and the left and right two pieces are passed through the positioning lock hinge The connection enables the huddle cylindrical space of the positioning lock to be effectively spliced; the positioning lock fastening screw is arranged on the left and right side of the positioning lock body, and the positioning lock fastening screw is tightened to make the positioning lock tightly entangled in the magnetic On the column of the base, loosen the positioning lock fastening screw, remove the positioning lock to the magnetic base column, and move the position of the positioning lock up and down.

[0051] A positioning lock silicone pad is arranged in the positioning lock body, and the positioning lock silicone pad and the magnetic base column are engaged, so that the positioning lock is fixed at a height position required by the magnetic base column.

[0052] the magnetic seat includes a soft magnetic material, and the soft magnetic material is disposed to separate the soft magnetic material into uniform upper and lower portions Two non-magnetic materials, a constant magnet is arranged in the cylindrical space surrounded by the soft magnetic material, and the constant magnet is connected with the constant magnet. When the constant magnet rotates to the off position, the constant magnet N The S pole is in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials separated by the non-magnetic material, and the magnetic seat adsorption surface is free from magnetism and can move freely; when the constant magnet rotates to the position of the crucible, the constant The N and S poles of the magnet are in the up and down position, and the N and S poles of the constant magnet are respectively aligned with the two soft magnetic materials separated by the non-magnetic material, and the iron perpendicular from the N pole to the soft magnetic material to the ultra clean table The side plate to another soft magnetic material to the S pole forms a magnetic line close, and the magnetic seat adsorption surface is adsorbed on the iron vertical side plate in the ultra clean table.

The method of use is the same as in the first embodiment.

Claims

Claim

[Claim 1] A magnetic base sterilization tray for a clean bench, comprising: a magnetic seat for generating an adsorption magnetic force, wherein a side of the magnetic seat is a vertical side plate for adsorbing the magnetic base on the iron in the clean bench The magnetic seat adsorption surface, the bottom surface of the magnetic seat is in contact with the bottom surface of the ultra-clean table, the upper surface of the magnetic seat is fixed with the column bottom plate, and the column is provided on the column bottom plate; the horizontal member height positioning lock is mounted on the column, and the horizontal member height positioning lock is arranged above the set a sterilizing tray horizontal member that is horizontally rotatable on the column; the sterilizing tray horizontal member includes a connecting sleeve for splicing the sterilizing tray horizontal member on the column of the magnetic base and assisting The horizontal component of the sterilization tray realizes a large-angle movement in the horizontal direction, and the connecting sleeve is connected with the horizontal placement plate of the culture plate on which the culture plate is placed through the horizontal arm, and the anti-slip frame is arranged around the horizontal placement plate of the culture plate to prevent the culture plate from slipping off.

[Claim 2] The magnetic base sterilization tray for a clean bench according to claim 1 or 2, wherein: the horizontal member height positioning lock comprises a positioning lock body for forming a positioning lock and a magnetic base column The positioning lock lock body is composed of two left and right pieces, and the left and right two pieces are connected by a positioning lock hinge, so that the cohesive cylindrical space of the positioning lock can be effectively kneaded; Set the positioning lock fastening screw at the mouth end, tighten the positioning lock fastening screw to make the positioning lock tightly fit on the column of the magnetic base, loosen the positioning lock fastening screw, remove the positioning lock to the magnetic base column, and can be up and down Move the position of the positioning lock arbitrarily.

[Claim 3] The magnetic base sterilization tray for a clean bench according to claim 2, wherein: a positioning lock silicone pad is arranged in the positioning lock body, and the positioning lock silicone pad and the magnetic base column are engaged to each other. The lock is attached to the height position required by the magnetic base column.

[Claim 4] The magnetic base sterilization tray for a clean bench according to claim 1, 2 or 3, characterized in that

The magnetic base comprises a soft magnetic material, and the soft magnetic material is provided with a non-magnetic material that separates the soft magnetic material into uniform upper and lower halves, and a constant magnet is arranged in the cylindrical space surrounded by the soft magnetic material, The magnet and the constant magnet are connected to each other. When the constant magnet rotates to the off position, the N and S poles of the constant magnet are in a horizontal position, and the magnetic lines of force are formed in two soft magnetic materials separated by the non-magnetic material. Closed circuit, the magnetic seat adsorption surface is not magnetic, and can move freely; when the constant magnet rotates to the 幵 position, the N and S poles of the constant magnet are in the up and down position. The N and S poles of the constant magnet are respectively aligned with two soft magnetic materials separated by a non-magnetic material, from the N pole to the soft magnetic material to the iron vertical side plate in the clean bench to another soft magnetic material to S The pole is formed to close the magnetic field line, and the magnetic seat adsorption surface is adsorbed on the iron vertical side plate in the clean bench.

[Claim 5] The magnetic base sterilization tray for a clean bench according to claim 4, wherein:

Pretreatment: Wipe the components of the magnetic base sterilization tray with disinfectant to ensure that the magnetic base sterilization tray installed in the clean bench does not change the aseptic environment in the clean bench;

Installation of the magnetic base: Rotate the constant magnet to the closed position; here, the N and S poles of the constant magnet are in a horizontal position, and the magnetic lines of force form a closed circuit in the two soft magnetic materials separated by the non-magnetic material. The magnetic seat adsorption surface is not magnetic and can move freely; the magnetic base is placed at the desired position, and the specific operation is as follows: the magnetic seat adsorption surface is in contact with the iron vertical vertical plate in the ultra-clean platform, the magnetic base bottom surface and the ultra-clean platform The bottom surface contacts; the rotating constant magnet rotates to the position of the crucible, the N and S poles of the constant magnet are in the upper and lower positions, and the N and S poles of the constant magnet are respectively aligned with the two soft magnetic materials separated by the non-magnetic material. From the N pole to the soft magnetic material to the iron vertical side plate in the ultra clean table to another soft magnetic material to the S pole, forming a magnetic line close, and the magnetic seat adsorption surface firmly adheres to the iron in the ultra clean table Vertical side panel;

Installation of the height positioning lock: Loosen the positioning lock fastening screw, put the cylindrical hole between the positioning locks into the column, select the required height, tighten the positioning lock fastening screw, and make the silicone pad of the positioning lock tightly fit the column. ;

Installation of the horizontal member of the sterilization tray and placement of the culture plate: take the horizontal member of the sterilization tray, and insert the connecting sleeve of the member into the column, and the lower edge of the connecting sleeve is located at the upper edge of the height positioning lock to complete the height positioning; The diameter of the inner ring of the connecting sleeve is slightly larger than the outer diameter of the column, so that the connecting sleeve can be freely rotated on the column without causing a significant swing on the column; thus, the magnetic base sterilization tray has been completed. Installation in the clean bench; According to the experiment, rotate the position of the plate horizontally, and place the plate and the plate cover in the space surrounded by the non-slip frame. The inner surface of the culture hole and the plate cover should be faced. UV lamp; sterilize them by using the UV lamp in the clean room; after the sterilization process is finished, Remove the UV lamp, cover the culture plate cover under aseptic conditions, remove the sterilized culture plate from the sterilization tray, and set aside;

Disassembly: Remove the magnetic base sterilization tray in the clean bench, move the horizontal component of the sterilization tray vertically upwards to disengage it from the column, remove the horizontal component of the sterilization tray, loosen the locking screw of the positioning lock, and move vertically upwards. Remove the height positioning lock from the column, and finally rotate the constant magnet to the closed position and take out the magnetic base.