WO2017022967A1

WO2017022967A1 - Composition and microcapsule for preventing citrus melanose, both containing lysimachiae foenum-graeci herba extract as active ingredient, and method for preventing citrus melanose by using same

Info

Publication number: WO2017022967A1
Application number: PCT/KR2016/007469
Authority: WO
Inventors: 이영진; 서인현; 김현우
Original assignee: 남부대학교산학협력단; 주식회사 현바이오
Priority date: 2015-07-31
Filing date: 2016-07-11
Publication date: 2017-02-09
Also published as: CN107231788A; KR101781635B1; KR20170014996A

Abstract

The present invention relates to a composition and a microcapsule for preventing citrus melanose, both containing a Lysimachiae Foenum-Graeci Herba extract as an active ingredient, and a method for preventing citrus melanose by using the composition. The composition, of the present invention, for preventing citrus melanose, containing a Lysimachiae Foenum-Graeci Herba extract as an active ingredient has an excellent preventive effect against citrus melanose and has very high potential, as a pollutant-free ingredient originating from a plant extract, to be developed into a biopesticide.

Description

Composition for the control of citrus fruit black spot pattern bottle containing Yeongneung-hyang extract as an active ingredient, microcapsules and method for controlling citrus fruit black spot pattern bottle using the same

The present invention is Yeongneung ( Lysimachiae) Foenum - Graeci Herba) relates to a composition for controlling tangerine black spot disease, comprising microcapsules and extracts as an active ingredient, and a method for controlling tangerine black spot disease using the composition.

Black spot disease (Melanose) is the most damaging disease for citrus farmers in Jeju Island. In severe areas, more than half of the total number of pesticide sprayings is done to control black spot disease.

In particular, the southeastern part of Jeju Island, including Seogwipo, is more affected by black spot disease than other regions. In addition, black spots are the cause of resin disease and the main storage disease of scab rot disease, the entire stem or tree suddenly dies.

The host range is citrus plants, and it occurs in all citrus fruits, but there are some differences in the onset depending on the type. Although it is widely distributed in the citrus region, the incidence is relatively low in areas where there is little rainfall during the growing period of citrus fruits, and in southeastern Jeju Island where rainfall is high.

The black-spotted pathogen is a fungus belonging to the fungal bacterium. The genital spore generation is Diaporthe citri F. A. Wolf and the conidia generation is Phomopsis citri Fawcett. The acute angle forming the conidia, the conidia, mainly occurs on dead branches, especially on recently dead branches, black oval or round, and the size is 200-450 μm. Bottle spores form two forms, alpha (α) spores and beta (β) spores. Alpha spores are colorless and monofoam, 5 ~ 9 × 2 ~ 4㎛ in size, which directly invade host plants, while beta spores are colorless, It is a silk-shaped thread, and its size is 20 ~ 30 × 0.7 ~ 1.5㎛ and it doesn't germinate so it doesn't directly invade host plants.

The despoilers erupt from the crustacean in the form of sticky yellow mucus in moist conditions and in the form of tendrils under relatively dry conditions, and spores from these are known to be very resistant to drying. Asympias occurs on the branch almost finished with crustacean formation. It is black and has a long beak shape. The tip is tapered and its length is 200 ~ 800㎛. Ascima is a long club, containing 8 capspores, each of which is colorless, 2, and two gulutulae, 11 ~ 15.5 × 3 ~ 5㎛. Optimal temperature for mycelial growth is 24

It starts germination after 4 hours at 20 ~ 28 ℃ but requires 10 hours at 15 ℃.

In the occurrence of black spot disease, pathogens overwinter in the form of sickle or asymptomatic, and infectious agents are diseased spores and vesicles produced from them. Although follicles scatter in the air and can spread pathogens over long distances by wind, their relative weight as infectious agents is relatively lighter than that of spores. Morphos are produced from the awakenings from dry branches on trees or dead branches left in orchards and scattered with raindrops. In the case of fruits, the disease can develop from the time of fall until 5 months after the fall, and the degree of disease varies depending on the amount of dead branches remaining in the water, the size of fruit (growing stage), and the period of wetting due to rainfall or dew. Do.

Pathogens settle on dead or pruned branches and form spores in two to three months, becoming infectious agents, and spore production continues for about a year. Pathogens that invade greenery are often alive without dying, and after the greenery dies (not sure if it dies by pathogens), pathogens become infectious agents. When spores invade fruits or leaves, host plants secrete antimicrobial substances as a defense against pathogens, killing invading pathogens, and reacting with them to form black spots.

Spores need to be wetted for more than 8 hours at 24 ~ 28 ℃ and more than 12 hours at 20 ℃. The incubation period is 1 ~ 2 days at 25 ℃ and 7 days at 10 ℃. Therefore, even if there is not much rain, it is a condition sufficient for the occurrence of the disease when the night temperature is somewhat high in the rain and in the dry state in the afternoon. If the disease condition is met, the disease will occur until early October, but the disease is rare since it is resistant after fruit hypertrophy except in special cases.

Symptoms of black spot disease appear only about 1 week after the invasion of pathogens. They develop on leaves, branches, and fruits, and vary widely depending on the time of infection, the condition of the fruit, and the climatic conditions at the time of infection. There are three types, niobium type and platen type. Epidermal cells of plants invaded by pathogens necrosis up to six layers of cells from the invasion site and are embedded with hard, dark red rubbery material, which is a representative sunspot. The affected area before the inflorescence is slightly recessed, and in some cases the depression is maintained until the harvest season, and the area remains blue even when pigmented and then gradually progresses. The size of the sunspots varies so much that during the infall phase, the affected lesions are relatively large in size, and in the more mature state, the lesions form relatively small lesions, and the shape of the sunspots becomes a distinct small bump, and the fruit's hypertrophy is almost over. Infected tissues are reddish in lesion and relatively unspotted. In addition, when the pathogen concentration is high, it becomes a dark red scab or swell-like shape.

In the case of leaves, the leaves start to be infected before the leaves are hardened in early and mid-May, and are typically embedded with black spots, but in severe cases, the leaves are yellowed, severely twisted, and become deciduous. In the case of stems, the young buds or greenery have been infected since the beginning of May and around mid-May.

As a method of controlling such black spot pattern disease, first, dead branches and pruning branches are removed. Because black spots are transmitted by spores formed from dead branches, if there are many infectious agents, that is, dead branches, no amount of pesticides can be used to control them. Thoroughly remove dead branches left on the tree during harvesting or pruning, and pruning branches can be burned or crushed to eliminate disease sources rather than stacked around orchards.

Drug control varies depending on the region, but the drug is sprayed four to five times every 15 to 20 days or between 200 and 250 mm of accumulated rainfall between mid-May and late-May to late-August. Drugs that have been announced include Mankoji (Daisen M-45), Dich (Delanic Hydrating), Mechiram (Sunup Granular Hydrating), and Propy (Anthracol Hydrating). . However, there are currently no eco-friendly drugs that can be used in pesticide-free citrus farmers.

Recently, a number of biological control methods using microorganisms and various extracts have been studied. Since these methods do not cause problems such as environmental pollution, resistance induction, residual toxicity, etc., they can be used in non-pesticide citrus farmers and are considered to be very desirable control methods. have.

Biomaterials that can control phytopathogens include antagonistic microorganisms, plant extracts, and antimicrobial oils. Antagonist microorganisms include Bacillus subtilis, Bacillus pumilus, Bacillus Licheniformis, Paenibacillus polymyxa, Ampelomyces quisqualis, Streptomyce genus, etc. There is no announced microbial pesticide. As plant extracts are known to have a sterilizing effect on phytopathogens such as squirrel, purslane, wisteria blotch, turmeric, etc., but it is not commercialized as an effective product yet. In addition, antimicrobial oils include thyme oil, clove bud oil, rose geranium oil, sassafras oil, castor oil, etc., and are known to have a bactericidal effect, but these also do not show satisfactory control values for single use.

Biopesticides are developed using the components of these plants, insecticides have been commercialized a lot, but fungicides are relatively weak and the situation is not varied. In addition, there is a lack of formulation technology capable of properly exerting an effect in the formulation of plant extracts and other mixed ingredients, the effect is weak compared to chemical pesticides or synthetic fungicides. In addition, in order to improve farm incomes and secure stable profitability, it is necessary to develop technologies that can produce crops in an environmentally friendly manner.

In tangerines, pests such as black spots, lancets, and ulcers, and mites, aphids, worms, and whiskers are causing great damage. Among them, environmentally friendly control agents for pest control have been developed and sold in 7 ~ 8 companies in Korea, the effect is known to be very excellent. The damage of citrus disease is about 80% of black spot disease, about 5% of window disease, about 10% of ulcer disease, and about 5% of other diseases. Therefore, development of biopesticides for black spot disease control is urgently needed to produce no pesticide citrus. Do.

Therefore, the present invention was developed to reduce the high-rise of farmers who want to grow pesticide-free citrus by developing an environmentally friendly fungicide that can control the black spot pattern disease that is the most damaging in the pesticide-free citrus cultivation.

On the other hand, the stem of Yeongneunghyang is about 70 ㎝ in height, the leaves are alternate, and the leaves are double-leaved in the Primulaaceae. Edo is known to have analgesic, anti-inflammatory and whitening effects in oriental medicine.

Thus, although the effect as a medicinal plant of Youngneung is known, the antimicrobial effect has not been disclosed or implied in any literature or patent so far.

As a result of the study to develop a natural fungicide for citrus black spot disease, the present inventors have studied the control activity against citrus black spot pattern disease using extracts of a number of natural medicines. It was found that the present invention has been completed.

The technical problem to be solved in the present invention is to provide a composition for controlling citrus black spot disease as a plant-derived material excellent in germicidal effect against citrus black spot disease as an active ingredient.

In addition, another technical problem to be solved in the present invention is to provide a microcapsule for controlling citrus black spot pattern bottle prepared by microcapsulating the composition.

In addition, another technical problem to be solved by the present invention is to provide a method for controlling the citrus black spot disease by using the composition for controlling the citrus black spot disease of the present invention.

In order to solve the above technical problem, the present invention provides a composition for controlling citrus black spot disease containing the extract of Lysimachiae Foenum-Graeci Herba as an active ingredient.

According to one preferred embodiment of the present invention, the yeongreunghyang extract is characterized in that the methanol extract of Yeongreung yang, the yeongreung yang extract is filtered after extracting Yeongneung incense with methanol for 12-36 hours at 40 ~ 60 ℃ One is preferable.

In order to solve the other technical problem described above, the present invention provides a microcapsule for controlling citrus black spot pattern disease containing the extract Yeongneung.

In order to solve the above another technical problem, the present invention provides a method of controlling citrus black spot pattern disease using the composition for controlling citrus black spot pattern bottle containing the extract Yeungneung.

Composition for the control of citrus black spot pattern bottle containing Yeongneung-hyang extract of the present invention as an active ingredient is not only excellent control effect for citrus black spot pattern bottle but also very high development potential as a biopesticide as a pollution-free component of plant extract origin.

1 is a scanning electron micrograph of a microcapsule formulation of the extract of Youngneung according to the present invention.

Figure 2 is a black spot pattern pathogen of Youngneunghyang extract ( Diapothe citri ) shows the results of measuring the antimicrobial activity.

Figure 3 shows the photo of the tangerine black spot disease control test packaging and drug treatment status.

Figure 4 is a photograph showing the drug efficacy test for citrus black spot disease of the microcapsules of the extract of Yeongneung and Youngneung in accordance with the present invention.

The present invention is to provide a composition for controlling citrus black spot disease containing Yeongneung (Lysimachiae Foenum-Graeci Herba) extract as an active ingredient.

The following drawings, which are attached to this specification, illustrate preferred embodiments of the present invention, and together with the contents of the present invention serve to further understand the technical spirit of the present invention, the present invention is limited to the matters described in such drawings. It should not be construed as limited.

The present invention provides a composition for controlling citrus black spot disease, which contains as an active ingredient the extract of Yeongneung-hyang showing control against citrus dark spot disease.

The youngneunghyang extract is a methanol extract of youngneunghyang. Myeongneung extract of Yeongneung can be obtained by grinding completely dry Yeongneung incense with a pulverizer, mixing the powder sample with 95% methanol in an appropriate ratio and stirring extraction. For example, the mixing ratio of the Youngneung sample and methanol is preferably 1: 6 (w / v). Extraction method is not particularly limited, preferably 12 to 36 hours at 40 ~ 60 ℃, more preferably at 50 ℃ for 24 hours to extract the filter can be used after concentration.

Citrus black spot disease control composition of the present invention as an active ingredient is included in the content of 1 to 80% by weight of Youngneung extract is confirmed to be effective in controlling citrus black spot disease. However, this may be appropriately adjusted in consideration of the degree of growth of citrus fruits, arable land environment, the extent of the development of black spots disease.

In addition, the composition for controlling citrus black spot disease, which contains the Youngneung extract of the present invention as an active ingredient, is a carrier, diluent, emulsifier, dispersing agent or interface necessary for formulation of excipients or adjuvants which are commonly used in insecticides or fungicides, such as pesticide formulation. Active agents and the like.

In addition, it can be formulated according to conventional formulation methods in the art, and can be prepared in various forms of control, for example, in a form suitable for control methods such as spraying directly on crops, such as liquid or powder.

As such, the composition for controlling citrus fruit black spot disease containing the extract Yeongneung-hyang extract is tested for efficacy through the control activity of citrus fruit black spot disease. In general, after evaluating the antimicrobial activity of the disclosed strains of citrus black spot disease can be confirmed through the pot test.

In an embodiment of the present invention, black spot pattern pathogen ( Diapothe citri ) is used as a causative strain of citrus dark spot pattern disease.

The antimicrobial activity of Yeongneung-si can be generally performed using both the Disk Paper Method and the Pour Plate Method. Briefly, the disk paper method is to spread the microbial preculture on a solid medium as known in the art, and put the antimicrobial-containing disk to be tested on the microbial plated flat medium in close contact and incubated around the disk. Clear zones can be measured to assay antimicrobial activity. Infusion plate method is to inject a certain amount of the antimicrobial sample to be tested in the liquid medium, solidified and inoculated by inoculating the antimicrobial strains, and by counting the number of growth strains to compare the antimicrobial effect can analyze the antimicrobial efficacy of the antimicrobial sample have.

Yeongneung- hyang extract-containing composition of the present invention confirmed the efficacy by such an analysis is a black spot pattern pathogen that is the causative strain of citrus black spot pattern disease ( Diapothe citri ) antibacterial activity.

As demonstrated through the following examples, the youngneung methanol extract according to the present invention is a black spot pattern pathogen ( Diapothe The inhibitory activity against citri ) shows excellent antibacterial effect in the Disk Paper Method. The preparation and antimicrobial activity assays of these extracts will be described in more detail in the Examples below.

In addition, the present invention can be microencapsulated in the following process the Youngnyeonghyang extract of the present invention.

Microencapsulation process of the present invention is largely (1) a starch aqueous solution preparation step; (2) adding natural plant extracts and emulsifying; (3) polymerization and microcapsule slurrying; (4) It can be divided into spray drying step.

Hereinafter, each step will be described in detail.

(1) starch aqueous solution preparation step

Since starch used as wall material is biodegradable, it is carried out at room temperature, and this property is characterized by the overall solubility of the encapsulated particles. The mass ratio of the drug contained to the aqueous solution of starch used as the coating material is maintained at 10 to 50: 1. Depending on the concentration of the starch aqueous solution, the density of the coating material may appear differently. In the present invention, a concentration of 10 to 90% is used.

The starch used is substituted with n-octenyl succinate, which combines exceptionally good emulsion properties and film properties in food starch and is generally effective for encapsulation of flavors. Generally, starch is treated with cyclodicarboxylic acid anhydride, where R represents a dimethylene or trimethylene radical, and R 'is a substituted group of alkyl, alkaenyl, araltyl or arakenyl groups with 5 to 18 carbon atoms It includes. In all these cases, the hydrophilic groups have the remaining carboxyl group (COOH) from hydrolysis in one of the carbon number groups. The n-octenyl succinate is at least 0.1 to 10% starch derivative, which is used to make products that are characterized by emulsification, coating and clouding. These derivatives depend on molecular weight with anhydrides added to about 0.1-30% starch. The pH of starch substituted by cyclic dicaboxylic acid anhydride reaction is about 5-11. The characteristics of this starch are modified by acid treatment and some breaking properties to make high fluidity. To make encapsulant, it is well dispersed in water and to form film with proper HLB value. The internals are provided in a limited release and dried powder.

Starch is n-octenyl succinate and has a molecular weight of 50,000 to 150,000. It has good emulsification stability for oil and shows a stable result even in the spray process for powdering microcapsules. The powder obtained in this way expands the dried coating material when water is added, and the oil inside leaks, and the starch is naturally decomposed. 10 to 90% of the starch solution has a pH value of 3 to 7 and the final microcapsules have a viscosity of 50 to 500 cps.

(2) Yeongneung-hyang extract addition and emulsification step

Add 0.1 ~ 100g of Yeongneung Hyang extract to 100g of starch aqueous solution 10 ~ 90%, and emulsify for several minutes at 1000 ~ 15000rpm using an emulsifier. At this time, the role of starch has the nature of emulsification and dispersion. Starch, because the process of extract Yeongneung-hyang is carried out at room temperature, it can be seen that the heat is generated poorly in the emulsification. At this time, the viscosity of the starch aqueous solution is important because the specific gravity of the Youngneung extract having a bactericidal and insecticidal effect is greater than that of water, and in the present invention, when preparing the starch aqueous solution of step (1), 0.1 to 20 g of NaCl is added to the starch. Increase the density of the aqueous solution.

(3) polymerization and microcapsule slurrying step

After emulsifying through the above steps, 0.1-30 g of a glutaraldehyde solution is added for the purpose of crosslinking of starch, and maintained at 1,000-15,000 rpm, followed by stirring for several minutes. In order to maintain the shape and size of the prepared microcapsules, polymerization is carried out with a homogenizer.

(4) spray drying step

The microcapsules made in the step (3) introduced a continuous spray drying process, avoiding a long time, because the Yeongneung extract is slightly out of stability in the slurry phase out.

In the case of the conventional spray drying food starch may be tested without the addition of a crosslinking agent, there is a problem that depends on the characteristics of the internal material. In the use of food starch, the substitution rate of the noctenyl succinate is 0.001 to 0.01 due to the limitations used in food.

Spray drying in the present invention, the temperature of the hot air fan is set to 150 ~ 250 ℃ and the temperature of the nozzle portion is maintained at 100 ~ 150 ℃. The cyclone portion is 40-100 ° C. The nozzle is sprayed with a microcapsule slurry having a size of 1 to 5 mm, and there are many microcapsules attached to the chamber, but it can be recovered and used as a result of small capsules agglomerating with each other.

In addition, the present invention provides a method for controlling citrus black spot pattern disease containing the extract of Yeongneung-hyang, or a control method of citrus black spot disease using a microcapsule comprising the extract of Youngneung-hyang.

In such a control method, the composition for controlling citrus black spot disease of the present invention can be used for controlling by the application method such as spraying directly on the foliar of the crop, air spraying or the like, as used in conventional control operations. In one embodiment, the control composition may be sprayed onto the crop at a concentration of 0.005% to 0.01%, but the control concentration may be reduced depending on the disease and incidence of the crop.

Based on the antibacterial effect of the citrus black spot disease, the Yeongneung-hyang extract is considered to have high agricultural applicability as an active ingredient of natural fungicides and even pesticides.

Hereinafter, the present invention will be described in detail by the following examples. These examples are merely illustrative of the invention and are not intended to limit the scope of the invention.

Example 1 Preparation of Youngneung Hyang Extract

Yeongneunghyang used in this experiment was purchased as a domestic specialty product at Gyeongdong Market. The completely dried Yeongneung was crushed with a grinder (HMF-340, Hanil, Korea) and used as a sample for extraction. Methanol (HPLC grade, Burdick & Jackson, USA) was used as an extraction solvent, and the Youngneung powder sample and methanol were mixed at a ratio of 1: 6 (w / v) and stirred three times at 50 ° C. for 3 hours. It was. The extract was filtered with filter paper (Whatman, No. 2) and concentrated under reduced pressure on a 45 ° C. water bath using a rotary evaporator (N-1000, Eyela, Japan). The concentrated Yeongneung was dissolved in DMSO (dimethyl sulfoxide) and used for antibacterial experiments. At this time, the final concentration of DMSO was not to exceed 1% (v / v) of the suspension.

Example 2 Microencapsulation of Youngneung Extract

The microcapsules containing the Youngneung hyang extract were prepared by microencapsulating the Youngneung extract according to the following steps.

Step 1: Dissolve 43 g of n-octenyl-substituted food starch (substitution rate 0.005) in 55 g of water. To this solution is added 4 g of NaCl to increase the density of starch. Prepare an aqueous solution of starch that is well dissolved in a single phase.

Step 2: 15g of Youngneung extract was added to the 45% starch aqueous solution of step 1, and stirred at 5000 rpm for 3 minutes using a homogenizer at high speed. The emulsion is emulsified in order to obtain uniform and small sized particles.

Step 3: 4 g of glutaraldehyde serving as a crosslinking agent was added to the mixed solution of step 2 (a solution obtained by emulsifying 10 g of Yeongneung extract in 100 g of water in a 45% starch aqueous solution) and crosslinked at 7000 rpm for 20 minutes.

Step 4: The obtained microcapsule solution is introduced into a spray drying process to obtain microcapsules in powder form. The microcapsule slurry was transferred to 20 ml / min or less by a pump, and at the same time, the air compressor was blown at 7 to 9 bar to prepare a microcapsule type soil disinfectant and insecticide.

Test Example 1 Antibacterial Activity of Youngneung Hyang

The strain used in this experiment was a black spot pattern pathogen ( Diapothe citri ), PDA (Potato Dextrose agarose) medium and cultured in a shaker incubator for 7 days at 25 ℃ condition and diluted with 0.85% saline to adjust the final concentration of the strain to 10 ⁶ ~ 10 ⁷ CFU / ml.

Precultured black spotted pathogen citri ) was prepared by smearing 1 ml each on PDA (Difco) plate medium using a smearing stick, and the Yeongneung samples were completely dried by absorbing 8 mm disc paper with sterile tweezers for each concentration. The dried disc was intimately adhered to a plate medium coated with a black spot pattern pathogen ( Diapothe citri ) and then cultured in a 25 ° C. incubator for 24 hours to measure the antibacterial activity by concentration. The results are shown in FIG. Figure 2 is a black spot pattern pathogen of Youngneunghyang extract ( Diapothe citri ) shows the results of measuring the antimicrobial activity.

As shown in Figure 2, in the antimicrobial activity test of Yeongneung-hyang extract through the disk clear zone check was able to confirm significantly higher antimicrobial activity than the non-treated (N.T).

<Test Example 2> Test for controlling citrus black spot disease

Yeongneung-hyang extract of the present invention was commissioned by Korea Institute of Plant Environment in 2015 to test the control effect of citrus black spots. The summary of the test process and results are as follows. This study was conducted in accordance with "Pesticide Item Registration Test Criteria and Methods" in order to test the effects of Yeongneung-hyang extract and Yeongneung-hyang extract on the citrus black spot disease of microcapsules. The effect was evaluated by comparing the fruit trees with no treatment.

In order to carry out this test, the test batches were tested in three complete randomized batches, and the evaluation of the efficacy test of the test was carried out to examine the morbidity of 100 fruits per ward after 10 days of the final drug treatment. We investigated whether there was weakness after baseline and volume treatment. Significant differences between drugs were tested by Duncan's multiple range test (DMRT) at 95% level.

Examination process

end. Disease: Black spot disease ( Diapothe citri )

I. Test crops (varieties): Tangerines (Gungcheon)

All. Treatment contents

TABLE 1

la. Drug treatment status

TABLE 2

hemp. Overview

Cultivation: Field Cultivation, Mulching Cultivation

Crop age: 35 years

Planting Distance: 3X3 m

Other drug treatments during the test period: No mixtures or other drug treatments.

bar. Test Method and Area: 3 Repeated Random Arrangement

TABLE 3

four. Weather situation before and after drug spraying

TABLE 4

In the second treatment, there is no rainfall in the test packaging, so the medicine is treated.

Ah. Test packaging and drug treatment status

Figure 3 shows the photo of the citrus control test packaging and drug treatment status.

character. Survey Method and Judgment Criteria

TABLE 5

Test score

end. Drug test

Drug Control Effects on Citrus Black Spot Patterns (Day 10 after Final Drug Treatment)

TABLE 6

I. Weakness test (3, 5, 7 days after drug treatment)

TABLE 7

Test result

end. Medicinal effect

The microcapsules of the Yeongneung-hyang extract and Yeongneung-hyang extract according to the present invention showed a good control effect, and showed a boiling control effect compared to the control agent. Figure 4 is a photograph showing the drug efficacy test for citrus black spot disease of the microcapsules of the extract of Yeongneung and Youngneung in accordance with the present invention.

I. Weak

The microcapsules of Yeongneung-hyang extract and Yeongneung-hyang extract according to the present invention did not have any weakness as a result of investigating the weakness after treatment with the standard amount and the quantitative amount.

As a result of the synthesis of the above tests, the untreated average disease rate of citrus black spot disease was 29.3%, which was a sufficient condition to evaluate the drug. After 10 days of treatment, 80.5% and 82.9%, respectively, showed a similar control effect compared to the control. In addition, as a result of examining the presence or absence of the leaves by treating the base amount and the amount of the microcapsules of the Yeongneung-hyang extract and Yeongneung-hyang extract according to the present invention, there was no weak.

In conclusion, this test was found to be statistically significant at least 80% compared to the untreated and untreated group, and there is no weakness in both the standard amount and the quantitative bar. It is thought that there is a significant control effect against spot pattern disease.

Composition for the control of citrus black spot pattern bottle containing Yeongneung-hyang extract of the present invention as an active ingredient is not only excellent control effect for citrus black spot pattern bottle but also very high development potential as a biopesticide as a pollution-free component of plant extract origin. In addition, the composition for the control of citrus black spot pattern bottle containing Yeongneung-hyang extract as an active ingredient can contribute to the production of eco-friendly citrus fruits, inducing consumption expansion and mass production of eco-friendly citrus fruits, and seeking to occupy the domestic market and export overseas. It is expected to be.

Claims

Composition for the control of citrus black spot pattern disease containing the extract of Yeongreung (Lysimachiae Foenum-Graeci Herba) as an active ingredient.
The method of claim 1,

The youngneung hyang extract is a composition characterized in that the youngneung hyang methanol extract.
The method of claim 1,

The Yeongneung hyang extract is characterized in that it is prepared by filtering after extracting Yeongneung incense for 12-36 hours at 40 ~ 60 ℃.
The method of claim 1,

The youngneunghyang extract is black spot pattern pathogen ( Diapothe citri ), characterized in that it exhibits bactericidal activity against.
Microcapsules for preventing tangerine black spots, including the extract of Youngneung.
Method for controlling citrus black spot pattern disease using the composition for controlling citrus black spot pattern bottle according to any one of claims 1 to 4.