WO2017012457A1

WO2017012457A1 - Grain storage method

Info

Publication number: WO2017012457A1
Application number: PCT/CN2016/087974
Authority: WO
Inventors: 姜标; 张琛; 陶黎明
Original assignee: 中国科学院上海有机化学研究所; 华东理工大学
Priority date: 2015-07-19
Filing date: 2016-06-30
Publication date: 2017-01-26
Also published as: CN106343013A

Abstract

A grain storage method comprises the following operation: placing a fumigant below or/and around a grain pile at an environment of the room temperature to the temperature of 40°C, the fumigant being a mixture of thiosulfinates compounds and thiosulfonates compounds. The present method not only safely and effectively sterilizes and disinfects grains, but also has the advantage of environmental protection. Particularly, multiple grains and various bacteria and moulds can be treated at a time under the same fumigating environment, and the fumigated grains can be placed for at least six months under granary environments or transportation conditions without regeneration of bacteria and moulds. In addition, the present method also has the advantages of simple operation, low cost, wide applied grain variety and bacteria-mould range, and the like, and has an important value and far-reaching significance to safe and long-term grain storage.

Description

Food storage method

Technical field

The invention relates to a food storage method and belongs to the technical field of food preservation.

Background technique

According to the National Bureau of Statistics, in 2014, the national total grain output was 60,709,900 tons (1.22 billion jin), an increase of 5.16 million tons (10.32 billion jin) from 2013, an increase of 0.9%. Ren Zhengxiao, member of the National Committee of the Chinese People's Political Consultative Conference and director of the State Food Bureau, said in an interview with reporters during the CPPCC panel discussion in May this year: At present, China's annual food loss is about 100 billion kilograms, which is equivalent to China's first grain-producing province. - One year's grain output in Heilongjiang Province. When food is produced from the ground to the table, there is a loss of waste in every link. According to estimates, the total loss of waste in the storage, transportation, processing and other circulation links in China is about 70 billion jin, of which only about 40 billion jin is lost in the circulation. The annual food loss of postpartum mold in China cannot be underestimated, and fungal and toxin pollution poses a serious threat to China's food security. According to Liu Yang, a researcher at the Agricultural Products Processing Research Institute of the Chinese Academy of Agricultural Sciences, "China has invested hundreds of billions of dollars every year to strive for 1% increase in grain production, but the annual post-harvest loss caused by mildew is as high as 21 million tons, accounting for the country's total grain output. 4.2%, the direct economic loss is about 18 billion to 24 billion yuan."

In addition, mycotoxin contamination also seriously restricts China's foreign trade. In recent years, the frequent occurrence of safety incidents caused by mycotoxin contaminated food has not only seriously threatened the health of humans and animals in China, but also restricted the foreign trade of China's agricultural products. From the statistical analysis of the violations of edible agricultural products exported to the EU in the past 10 years from 2002 to 2011, it was found that among the many factors restricting China's foreign trade, such as heavy metals, agricultural and veterinary drug residues, the main cause of mycotoxin exceeding is the cause of mycotoxin. The number of violations reached 28.6%. It is understood that mycotoxins are the most dangerous food contaminants naturally occurring, and the toxicity of aflatoxins is 10 times that of potassium cyanide and 68 times that of arsenic, which is one of the most important causes of liver cancer. Mycotoxins are mainly produced during post-harvest food storage. In China's grain storage process, molds are mainly Aspergillus flavus, Fusarium oxysporum, and Fusarium graminearum. In Europe and the United States, except for mold, Aspergillus flavus and Fusarium graminearum In addition, there are parasitic Aspergillus and yellow Fusarium. At the same time, compared with the characteristics of short storage period, single source and small quantity of grain storage in Europe and the United States, the characteristics of grain storage in China are mainly characterized by high bacteria, long storage period, wide source, variety and quantity.

At present, most grain depots use methods such as grain application, bag burial and probe method for fumigation and insecticide. The fumigants used are mainly phosphine, ethylene oxide, methyl bromide and sulfuryl fluoride, which are environmentally friendly and safe. The improvement of consciousness, the limitations of the above fumigants gradually manifested, such as: phosphine poisoning, damage to the lungs, heart, liver, kidney, central nervous system and bones, excessive exposure to asthma, pneumonia or pulmonary fibrosis, And unsafe factors such as easy self-ignition; ethylene oxide Denaturation and explosiveness; the destructive effect of methyl bromide on the stratospheric ozone and the direct toxicity to humans have drawn great attention. Since the ninth meeting of the Conference of the Parties to the Montreal Protocol in 1997, developed countries have agreed to phase out the use of methyl bromide disinfectant since 2005. China also officially signed the Copenhagen Amendment to the Copenhagen Protocol in April 2003, promising to The use of methyl bromide in agriculture, warehousing, tobacco and other industries will be completely stopped by January 1, 2015. Of course, this commitment will also be implemented in food disinfection; sulfuryl fluoride is a colorless, toxic, and highly irritating gas at normal temperature and pressure, and acute toxicity mainly damages the central nervous system and causes convulsions; Deposited in human bones with a cumulative risk of poisoning. The latest research finds that the chemical fumigant sulfuryl fluoride, when released into the air, will become a potent greenhouse gas. One kilogram of sulfuryl fluoride is emitted into the atmosphere. The effect on global warming is 4,800 times that of one kilogram of carbon dioxide; now although in the air There is only a very small amount of sulfuryl fluoride, about 1.5 trillion, that is, only 1.5 of each mega air molecule is sulfuryl fluoride, but it is increasing at a rate of 5% per year, and it will be replaced by methyl bromide in the future. The atmospheric greenhouse effect caused by the application of sulfuryl fluoride should be given enough attention!

In summary, solving the problem of food mold has become a major demand for ensuring national food security. Exploring effective control methods for mycotoxins in China's grain storage has become an urgent research topic.

Summary of the invention

In view of the above problems and needs of the prior art, it is an object of the present invention to provide a green, safe and effective food storage method.

In order to achieve the above object, the present invention adopts the following technical solutions;

A food storage method comprising the steps of: placing a fumigant under or/and around a grain pile at room temperature to 40 ° C; the fumigant is a thiosulfinate compound and a thiosulfonate A compound of an acid ester compound.

Preferably, the fumigant is placed below or/and around the grain pile in a closed environment at room temperature.

Preferably, the fumigant is supported on a carrier material having an adsorption function, which may be filter paper, silica gel, dextrin, filter membrane, cotton, sponge or cloth, and the like.

Preferably, the carrier material loaded with the fumigant is placed in a hollow device having an outwardly diffusing port or mesh surface, and the hollow device may be a box structure, a box structure, a cage structure, a bag, or the like. .

As a further preferred solution, the hollow device has a fan and a speed control function.

As a further preferred embodiment, the hollow device has a self-heating and temperature regulating function.

Preferably, the fumigant is a compound having a volume ratio of 1:1 to 1:5 by a thiosulfinate compound and a thiosulfonate compound.

Preferably, the thiosulfinate compound has the following structural formula:

a compound wherein: R ¹ and R ^{2 are the} same or different and are each selected from a C ₁ -C ₄ alkane group (for example, methyl, ethyl, propyl, butyl) or a C ₂ -C ₄ alkene group. (Example: allyl).

As a further preferred embodiment, R ¹ is the same as R ² .

Preferably, the thiosulfonate compound has the following structural formula:

a compound wherein: R ³ and R ^{4 are the} same or different and are each selected from a C ₁ -C ₄ alkane group (for example, methyl, ethyl, propyl, butyl) or a C ₂ -C ₄ alkene group. (Example: allyl).

As a further preferred embodiment, R ³ is the same as R ⁴ .

Compared with the prior art, the present invention has the following beneficial effects:

The invention adopts a compound of a thiosulfinate compound and a thiosulfonate compound as a fumigant, which not only realizes safe and effective sterilization and disinfection of food, but also has the lowest environmental pollution and no fumigant residue. The problem does not affect the food itself and human health, and has the advantages of green environmental protection; in particular, the present invention can realize one-time treatment of various foods and various bacterial molds in the same fumigation environment, and after the fumigation treatment of the present invention The grain can be placed in the granary environment or transportation conditions for at least half a year to no longer regenerate the bacterial mold; in addition, the method of the invention has the advantages of simple operation, low cost, wide range of applicable grain varieties and bacterial molds, and safety for storing grain. Long-term preservation is of great value and far-reaching significance.

detailed description

The technical solution of the present invention will be further described in detail below by way of examples.

Example 1: Preparation of fumigant

1. A mixture of methyl methyl sulfinic acid sulfonate and ethyl ethyl thiosulfonate:

Methyl methylthiosulfinate and ethyl thiosulfonate were stirred and mixed at a volume ratio of 1:3 at room temperature; abbreviated as fumigant A; determination of oral LD ₅₀ >500 mg/Kg in mice It belongs to low toxicity.

2. Compound of ethyl ethyl sulfinic acid sulfonate and methyl methyl thiosulfonate:

Ethyl ethyl thiosulfinate and methyl methyl sulfonate were stirred and mixed at a volume ratio of 1:2 at room temperature; abbreviated as fumigant B; determination of oral LD ₅₀ >500 mg/Kg in mice It belongs to low toxicity.

3. Compound of ethyl thiosulfinate and ethyl thiosulfonate:

Ethyl ethyl thiosulfinate and ethyl thiosulfonate were stirred and mixed at room temperature 1:1 at room temperature; abbreviated as fumigant C; determination of oral LD ₅₀ >500 mg/Kg in mice It belongs to low toxicity.

4. Compound of methyl thiosulfinate and methyl methyl sulfonate:

Methyl methylthiosulfinate and methyl methylsulfonate were stirred and mixed at a volume ratio of 1:1.5 at room temperature; abbreviated as fumigant D; determination of oral LD ₅₀ >500 mg/Kg in mice It belongs to low toxicity.

5. A mixture of propyl thiosulfinate and ethyl thiosulfonate:

The propyl thiosulfinate sulfonate and ethyl thiosulfonate were stirred and mixed at a volume ratio of 1:2.5 at room temperature; abbreviated as fumigant E; the oral LD ₅₀ >500 mg/Kg was determined. It belongs to low toxicity.

6. Compound of propyl thiosulfinate sulfonate and allyl thiosulfonic acid allyl ester:

The propyl thiosulfinate propyl ester and allyl thiosulfonic acid allyl ester were stirred and mixed at a volume ratio of 1:4 at room temperature; abbreviated as fumigant F; determination of oral LD ₅₀ >500 mg in mice /Kg, which is low in toxicity.

7. A mixture of ethyl ethyl thiosulfinate and butyl butyl sulfonate:

Ethyl ethyl thiosulfinate and butyl butyl sulfonate were stirred and mixed at a volume ratio of 1:5 at room temperature; abbreviated as fumigant G; determination of oral LD ₅₀ >500 mg/Kg in mice It belongs to low toxicity.

8. A mixture of methyl thiosulfinate and methyl thiosulfonate:

Ethyl methylthiosulfinate and methyl thiosulfonate were stirred and mixed at a volume ratio of 1:3 at room temperature; abbreviated as fumigant H; determination of oral LD ₅₀ >500 mg/Kg in mice It belongs to low toxicity.

9. A combination of allyl thiosulfinate and allyl methylthiosulfonate:

Allyl allysulfinyl sulfinate and allyl methylthiosulfonate were stirred and mixed at a volume ratio of 1:2.5 at room temperature; abbreviated as fumigant I; determination of oral LD _{50 in} mice > 500mg/Kg, which is low in toxicity.

Example 2: Grain Fumigation Experiment

Grain variety: corn

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

Fumigation method:

The fumigant C is first loaded on the filter paper, and then the corn sample is placed on top of the filter paper (which can be achieved by setting a fixed frame) and fumigation at room temperature.

After sampling and monitoring, after 36 hours of fumigation, there was no mold growth (obtained by microscopic examination) on the corn sample treated by the above fumigation, and there was no fumigant residue on the corn sample after the above fumigation treatment ( It is detected by gas chromatography and mass spectrometry (GC-Ms).

The corn sample subjected to the fumigation treatment of the present embodiment is placed in a room temperature environment, and the mold tracking test is performed regularly. The test results are shown in the table below:

存放时间Storage time	0个月0 months	1个月1 month	3个月3 months	6个月6 months
霉菌生长情况Mold growth	无任何霉菌No mold	无任何霉菌No mold	无任何霉菌No mold	无任何霉菌No mold

In addition, it was observed by the color difference meter that the color of the corn sample subjected to the above fumigation treatment did not change significantly.

Example 3-1: Grain Fumigation Experiment

Grain variety: rice

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

First, the fumigant C is loaded on the filter paper, and then the filter paper is placed at the bottom of the glass cylinder, and then the rice sample is placed on top of the filter paper (can be realized by providing a fixed frame in the glass cylinder), the closed glass cylinder is at room temperature. Fumigation under.

After sampling and monitoring, it was found that after fumigation for 24 hours, there was no mold growth (obtained by microscopic examination) on the rice sample treated by the above fumigation, and there was no fumigant residue on the rice sample after the above fumigation treatment ( It is detected by gas chromatography and mass spectrometry (GC-Ms).

The rice samples subjected to the fumigation treatment of the present example were placed in a room temperature environment, and the mold tracking test was performed regularly, and the test results are shown in the following table:

In addition, it was observed by the color difference meter that the color of the rice sample subjected to the above fumigation treatment did not change significantly.

Example 3-2: Grain Fumigation Experiment

Grain variety: rice

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

The fumigant C is first loaded on the filter paper, and then the filter paper is placed in a hollow box having a mesh surface, a fan and a speed control function, and the box is placed at the bottom of the glass cylinder to prepare the rice sample. It is placed above the box (can be realized by providing a fixing frame in the glass cylinder), the glass jar is sealed, and the fan is turned on at room temperature (the wind speed is set to medium speed) for fumigation.

After sampling and monitoring, it was found that after fumigation for 18 hours, there was no mold growth (obtained by microscopic examination) on the rice sample treated by the above fumigation, and there was no fumigant residue on the rice sample after the above fumigation treatment ( It is detected by gas chromatography and mass spectrometry (GC-Ms).

The rice sample subjected to the fumigation treatment of the present embodiment is placed in a room temperature environment, and the mold tracking test is performed regularly. The test results are shown in the table below:

Example 3-3: Grain Fumigation Experiment

Grain variety: rice

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

The fumigant C is first loaded on the filter paper, and then the filter paper is placed in a hollow box having a mesh surface, self-heating and temperature-regulating function, and the box is placed at the bottom of the glass jar to prepare the rice sample. It is placed above the box (can be realized by providing a fixing frame in the glass cylinder), and the glass jar is sealed, and the temperature of the adjusting box is fumigation at 30 °C.

After sampling and monitoring, it was found that after the fumigation for 20 hours, there was no mold growth (obtained by microscopic examination) on the rice sample treated by the above fumigation, and there was no fumigant residue on the rice sample after the above fumigation treatment ( It is detected by gas chromatography and mass spectrometry (GC-Ms).

Example 3-4: Grain Fumigation Experiment

Grain variety: rice

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

The fumigant C is first loaded on the filter paper, and then the filter paper is placed in a hollow box having a mesh surface and self-heating function, and the box is placed at the bottom of the glass jar to place the rice sample. Above the casing (which can be realized by providing a fixing frame in the glass cylinder), the glass cylinder is sealed, and the temperature of the casing is adjusted to be 40 ° C for fumigation.

After sampling and monitoring, it was found that after fumigation for 10 hours, there was no mold growth (obtained by microscopic examination) on the rice sample treated by the above fumigation, and there was no fumigant residue on the rice sample after the above fumigation treatment ( It is detected by gas chromatography and mass spectrometry (GC-Ms).

Example 3-5: Grain Fumigation Experiment

Grain variety: rice

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

The fumigant C is first loaded on the filter paper, and then the filter paper is placed in a hollow box having a mesh surface, a fan and a temperature regulating function, and the box is placed at the bottom of the glass jar to prepare the rice sample. It is placed above the box (can be realized by providing a fixing frame in the glass cylinder), the glass jar is sealed, the temperature of the box is adjusted to 30 ° C, and the fan is turned on (the wind speed is set to low speed) for fumigation.

After sampling and monitoring, it was found that after fumigation for 8 hours, there was no mold growth (obtained by microscopic examination) on the rice sample treated by the above fumigation, and there was no fumigant residue on the rice sample after the above fumigation treatment ( It is detected by gas chromatography and mass spectrometry (GC-Ms).

From the experimental results of Examples 3-1 to 3-5, it can be seen that the airtightness of the closed environment and the fan and the appropriate heating are beneficial to the fumigation effect, and the fumigation time can be significantly shortened under the same fumigation effect.

Example 4: Grain Fumigation Experiment

Grain varieties: soybeans and other beans

Diseases: Aspergillus and Penicillium

Fumigant: Fumigant C

Fumigation method:

First, the fumigant C is loaded on the silica gel particles, and then the silica gel particles are placed in a bag having a mesh on the surface, and then the bag is placed at the bottom of the glass jar, and the bean sample is placed above the bag (through A fixed frame is provided in the glass cylinder), a closed glass cylinder, and fumigation at room temperature.

After sampling and monitoring, after 36 hours of fumigation, there was no mold growth (known by microscopic examination) on the fumigation samples, and there was no fumigant on the fumigation samples. Residue (reviewed by gas chromatography and mass spectrometry (GC-Ms) analysis).

The soybean samples such as soybeans subjected to the fumigation treatment of the present embodiment are placed in a room temperature environment, and the mold tracking test is performed regularly, and the test results are shown in the following table:

Further, the color of the bean sample such as soybean after the above-described fumigation treatment was not observed to be significantly changed by a color difference meter.

Example 5: Grain Fumigation Experiment

Grain varieties: potato, sweet potato and other potatoes

Diseases: Mycobacterium sphaeroides, Rhizopus and Trichoderma

Fumigant: Fumigant C

Fumigation method:

First, the fumigant C is loaded on the cyclodextrin, and then the cyclodextrin device is placed in a bag having a mesh on the surface, and the bag is placed at the bottom of the glass jar, and a potato sample such as potato or sweet potato is placed in the bag. Above (can be achieved by placing a fixed frame in the glass jar), sealed glass jar, fumigation at room temperature.

After sampling and monitoring, it was found that after fumigation for 30 hours, there was no mold growth on the potato samples treated by the above fumigation (known by microscopic examination), and there was no fumigant on the potato samples after the above fumigation treatment. Residue (reviewed by gas chromatography and mass spectrometry (GC-Ms) analysis).

The potato and sweet potato samples which have been subjected to the fumigation treatment of this embodiment are placed in a room temperature environment, and the mold tracking test is performed regularly, and the test results are shown in the following table:

In addition, the color of the potato sample such as potato and sweet potato after the above-mentioned fumigation treatment was not observed to be significantly changed by a color difference meter.

Example 6: Grain Fumigation Experiment

Grain varieties: wheat, barley and other wheat

Diseases: Aspergillus, Trichoderma and Trichoderma

Fumigant: Fumigant C

Fumigation method:

First, the fumigant C is loaded on the filter paper, and then the filter paper is placed at the bottom of the glass jar, and wheat samples such as wheat and barley are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass Cylinder, fumigation at room temperature.

After sampling and monitoring, it was found that after fumigation for 24 hours, there was no mold growth (known by microscopic examination) on the wheat sample treated by the above fumigation, and there was no fumigant on the wheat sample after the above fumigation treatment. Residue (reviewed by gas chromatography and mass spectrometry (GC-Ms) analysis).

The wheat samples subjected to the fumigation treatment of the present embodiment are placed in a room temperature environment, and the mold tracking test is performed regularly, and the test results are shown in the following table:

In addition, it was observed by the color difference meter that the color of the wheat sample after the above-mentioned fumigation treatment did not change significantly.

Example 7-1: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Diseases: Staphylococcus aureus, Escherichia coli, Bacillus megaterium, Bacillus subtilis, Pseudomonas fluorescens, etc.

Fumigant: Fumigant C

Fumigation method:

The fumigant C is first loaded on the filter paper, and then the filter paper is placed at the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

After sampling and monitoring, after 24 hours of fumigation, there was no mold growth (microscopic detection) on the wheat, rice and corn samples after the above fumigation treatment, and the wheat, rice and corn after the above fumigation treatment. There was also no fumigant residue on the sample (detected by gas chromatography and mass spectrometry (GC-Ms)).

The wheat, rice, and corn samples subjected to fumigation treatment in this example were placed in a room temperature environment, and the bacteria tracking test was performed periodically. The test results are shown in the following table:

存放时间Storage time	0个月0 months	1个月1 month	3个月3 months	6个月6 months
细菌生长情况Bacterial growth	无任何细菌No bacteria	无任何细菌No bacteria	无任何细菌No bacteria	无任何细菌No bacteria

In addition, the color of the wheat, rice, and corn samples subjected to the above-described fumigation treatment was not significantly changed by a color difference meter.

Example 7-2: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant A

Fumigation method:

First, the fumigant A is loaded on the filter paper, and then the filter paper is placed on the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

Example 7-3: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant B

Fumigation method:

First, the fumigant B is loaded on the filter paper, and then the filter paper is placed at the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

Storage time

0 months

1 month

3 months

6 months

Bacterial growth

No bacteria

Example 7-4: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant D

Fumigation method:

The fumigant D is first loaded on the filter paper, and then the filter paper is placed on the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

Example 7-5: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant E

Fumigation method:

First, the fumigant E is loaded on the filter paper, and then the filter paper is placed on the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

After sampling and monitoring, after 24 hours of fumigation, there was no mold growth (microscopic detection) on the wheat, rice and corn samples after the above fumigation treatment, and the wheat, rice and corn after the above fumigation treatment. On the sample There is also no fumigant residue (detected by gas chromatography and mass spectrometry (GC-Ms) analysis).

Example 7-6: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant F

Fumigation method:

The fumigant F is first loaded on the filter paper, and then the filter paper is placed at the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

Example 7-7: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: fumigant G

Fumigation method:

First, the fumigant G is loaded on the filter paper, and then the filter paper is placed at the bottom of the glass jar, and the wheat, rice, and jade are placed. The meter sample is placed above the filter paper (can be achieved by placing a fixed frame in the glass jar), sealed glass jar, and fumigated at room temperature.

Example 7-8: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant H

Fumigation method:

The fumigant H is first loaded on the filter paper, and then the filter paper is placed at the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

Example 7-9: Grain Fumigation Experiment

Grain varieties: wheat, rice, corn

Fumigant: Fumigant I

Fumigation method:

First, the fumigant I is loaded on the filter paper, and then the filter paper is placed on the bottom of the glass jar, and the wheat, rice, and corn samples are placed on top of the filter paper (can be realized by providing a fixing frame in the glass cylinder), the closed glass cylinder , fumigation at room temperature.

Example 8: Grain Fumigation Experiment

Grain varieties: corn, rice, wheat, soybeans, soybeans, broad beans, potatoes, sweet potatoes, etc.

Diseases: Aspergillus, Penicillium, Trichoderma, Staphylococcus aureus, Escherichia coli, Bacillus megaterium, Bacillus subtilis, Pseudomonas fluorescens, etc.

Fumigant: Fumigant C

Fumigation method:

First, the fumigant C is loaded on the filter paper, and then the filter paper is placed at the bottom of the glass cylinder, and the mixed food sample is placed above the filter paper (can be realized by providing a fixing frame in the glass cylinder), and the closed glass cylinder is Fumigation was carried out at room temperature.

After sampling and monitoring, it was found that after the fumigation for 24 hours, there was no mold growth (known by microscopic examination) on the above-mentioned mixed grain sample after the above-mentioned fumigation treatment, and there was no above-mentioned mixed grain sample after the above-mentioned fumigation treatment. Residue of fumigant (reviewed by gas chromatography and mass spectrometry (GC-Ms)).

The above-mentioned mixed grain sample subjected to the fumigation treatment of the present embodiment is placed in a room temperature environment, and the mold tracking test is performed regularly, and the test results are shown in the following table:

The color of the mixed grain samples after the above-mentioned fumigation treatment, such as corn, rice, wheat, soybean, soybean, broad bean, potato, sweet potato, and the like, were not changed significantly by the color difference meter. It is indicated that the method of the invention can realize one-time treatment of multi-species grain and various bacterial molds in the same fumigation environment.

It is to be understood that the above description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any technical person skilled in the art can disclose the technical scope disclosed by the present invention. Variations or substitutions that are conceivable within the scope of the invention are intended to be included within the scope of the invention.

Claims

A method for storing foodstuffs, comprising the steps of: placing a fumigant under or/and around a grain pile at room temperature to 40 ° C; said fumigant is a thiosulfinate compound A compound with a thiosulfonate compound.
A method of food storage according to claim 1 wherein the fumigant is placed below or/and around the grain pile in a closed environment at room temperature.
The food storage method according to claim 1 or 2, wherein the fumigant is supported on a carrier material having an adsorption effect.
A method of storing food according to claim 3, wherein the carrier material loaded with the fumigant is placed in a hollow device having an outwardly diffusing port or mesh face.
The food storage method according to claim 4, wherein the hollow device has a fan and a speed control function.
The food storage method according to claim 4 or 5, wherein the hollow device has a self-heating and temperature-regulating function.
The food storage method according to claim 1 or 2, wherein the fumigant is a 1:1 to 1:5 by volume ratio of the thiosulfinate compound to the thiosulfonate compound. The compound.
The food storage method according to claim 7, wherein the thiosulfinate compound has the following structural formula:
A compound wherein R 1 and R 2 are the same or different and are each selected from a C 1 -C 4 alkane group or a C 2 -C 4 alkene group.
The food storage method according to claim 7, wherein the thiosulfonate compound has the following structural formula:
A compound wherein: R 3 is the same as or different from R 4 and is each selected from a C 1 -C 4 alkane group or a C 2 -C 4 alkene group.