WO2017002906A1 - Composition for inhibiting alcohol absorption - Google Patents

Abstract

Provided are a composition for inhibiting alcohol absorption, use of the composition for inhibiting alcohol absorption, and a method for inhibiting alcohol absorption. It was found that a heat-treated product of a plant-derived peptide has a very high alcohol absorption inhibitory effect. The present invention provides a composition for inhibiting alcohol absorption containing the heat-treated product of the plant-derived peptide as an active ingredient, use of the heat-treated product of the plant-derived peptide for inhibiting alcohol absorption, and a method of inhibiting alcohol absorption by using the heat-treated product of the plant-derived peptide.

Description

Composition for suppressing alcohol absorption

The present invention relates to a composition for suppressing alcohol absorption. More specifically, the present invention utilizes an alcohol absorption suppression composition containing a plant-derived peptide heat-treated product as an active ingredient, the use of a plant-derived peptide heat-treated product for suppressing alcohol absorption, and a plant-derived peptide heat-treated product. The present invention relates to a method for suppressing alcohol absorption.

Alcohol taken from outside the body is absorbed mainly in the stomach and upper small intestine. Alcohol is absorbed without digestion. It is said that the absorption is generally fast, and if alcohol is consumed, alcohol in the digestive tract is almost absorbed in 1 to 2 hours after drinking. As the alcohol is absorbed, its decomposition begins quickly. However, it is known that the alcohol degradation rate varies greatly among individuals. Many factors are said to be involved in the absorption and degradation of alcohol.

ア ル コ ー ル Alcohol has the effect of relaxing feelings and increasing conversation if it is small, but if it is large, it has an anesthetic-like effect, paralyzing motor function and causing consciousness disturbance. Excessive alcohol intake may cause epilepsy or addictive symptoms. Alcohol is known to act on various parts of the body, and excessive alcohol intake may adversely affect the central nervous system, circulatory system, lipids, blood coagulation, endocrine secretion, and the like.

In order to reduce undesirable diseases and symptoms caused by alcohol consumption, there is a strong demand for the development of technology that suppresses absorption of alcohol taken from outside the body. As such a technique, for example, a technique using a dipeptide called glycylglycine is disclosed (Patent Document 1). However, there are not so many cases where alcohol absorption suppression technology has been reported so far, and there is a demand for a technology that is more effective and has high utility value.

On the other hand, as a technique for preventing the accumulation of blood alcohol after ingestion of alcohol, which is different from the absorption inhibition of alcohol, a technique using an enzyme-hydrolyzed product of corn protein (Patent Document 2), and an enzyme of soybean protein A technique using a hydrolyzed product (Patent Document 3) and the like are disclosed. These are all technologies related to alcohol metabolism promotion, and are technologies that focus on decomposition rather than alcohol absorption.

JP-A-3-12739 JP-A-7-285881 International Publication No. 2006/106704 Pamphlet

There is a time difference between alcohol absorption and decomposition, and furthermore, there are individual differences in enzyme activity related to alcohol decomposition, so alcohol absorption suppression that targets the early stage rather than promoting alcohol metabolism Development of a method is desired. An object of the present invention is to provide a composition for suppressing alcohol absorption, use of the composition for suppressing alcohol absorption, and a method for suppressing alcohol absorption.

As a result of intensive studies in view of the above problems, the present inventors have found that a heat-treated product of a plant-derived peptide can extremely effectively suppress the alcohol concentration in blood. Furthermore, the present inventors have found that such a plant-derived peptide heat-treated product does not particularly affect alcohol dehydrogenase activity and acetaldehyde dehydrogenase activity related to alcohol metabolism, and as a result, plant-derived peptide heat-treated products are extremely high. It is considered to have an alcohol absorption inhibitory effect, and the present invention has been completed.

That is, the present invention relates to the following, but is not limited thereto.
(1) A composition for suppressing alcohol absorption, comprising a plant-derived peptide heat-treated product as an active ingredient.
(2) The composition for suppressing alcohol absorption according to (1), wherein the content of the cyclic dipeptide of the heat-treated peptide derived from a plant or a salt thereof is 20000 μg / 100 g / Bx or more.
(3) The composition for suppressing alcohol absorption according to (1) or (2), wherein the content of the cyclic dipeptide or the salt thereof in the plant-derived heat-treated peptide is 30000 μg / 100 g / Bx or more.
(4) The composition for suppressing alcohol absorption according to any one of (1) to (3), wherein the plant-derived peptide is a peptide derived from malt, tea or soybean.
(5) The composition for suppressing alcohol absorption according to any one of (1) to (4), wherein the heat-treated product is a treated product at 100 ° C. or higher and 0.101 MPa or higher.
(6) The composition for suppressing alcohol absorption according to any one of (1) to (5), which is used for suppressing an increase in blood alcohol concentration.
(7) The composition for suppressing alcohol absorption according to any one of (1) to (6), which is used for treatment or prevention of a disease or symptom associated with an increase in blood alcohol concentration.
(8) The composition for suppressing alcohol absorption according to any one of (1) to (7), which is labeled with a function exhibited by suppressing absorption of alcohol.
(9) Function indications are “suppress alcohol absorption”, “moderate alcohol absorption”, “prevent hangover”, “friendly to the liver”, “protect the liver”, and “ The composition for suppressing alcohol absorption according to (8), which is selected from the group consisting of “maintaining liver function”.
(10) The composition for suppressing alcohol absorption according to any one of (1) to (9), wherein the composition is an agent.
(11) Use of a plant-derived peptide heat-treated product for suppressing alcohol absorption.
(12) A method for suppressing alcohol absorption, using a plant-derived heat-treated peptide as an active ingredient.

According to the present invention, it is possible to provide a composition having a very effective alcohol absorption inhibiting action. Since the plant-derived peptide heat-treated product contained in the composition of the present invention can be obtained from plants that can be used for food and drink, it can be said to have high safety and high utility value in the market. In addition, by utilizing the composition of the present invention, a method for effectively suppressing alcohol absorption can be provided, which can alleviate undesirable diseases and symptoms caused by alcohol consumption. .

FIG. 1 is a diagram showing the effects of heat-treated malt peptide and heat-treated tea peptide on blood ethanol concentration. FIG. 2 is a diagram showing the alcohol area value for 120 minutes obtained from the measurement result of blood ethanol concentration. The vertical axis of the graph represents the alcohol area value for 120 minutes after ethanol administration. FIG. 3 is a diagram showing the effect of heat-treated soybean peptide on blood ethanol concentration. FIG. 4 is a diagram showing the alcohol area value for 240 minutes obtained from the measurement result of blood ethanol concentration. The vertical axis of the graph indicates the alcohol area value for 240 minutes after ethanol administration. FIG. 5 is a diagram showing the relationship between the administration of a plant-derived peptide heat-treated product and the activity of an alcohol metabolism-related enzyme. ADH is an alcohol dehydrogenase and ALDH is an acetaldehyde dehydrogenase. The vertical axis of each enzyme graph represents enzyme activity, and the vertical axis of the blood ethanol AUC (Area Under Curve) graph represents the alcohol area value for 120 minutes after ethanol administration. Moreover, the horizontal axis of each graph shows a test sample, in which “tea EH” means a sample obtained by subjecting tea to an enzyme treatment and then a high-temperature and high-pressure treatment. FIG. 6 is a diagram showing the effect of heat-treated soybean peptide on human blood alcohol concentration. The vertical axis of the graph indicates blood ethanol concentration (mg / mL), and the horizontal axis of the graph indicates time (minutes) after ethanol loading. FIG. 7 is a diagram showing the alcohol area value for 180 minutes obtained from the measurement result of the human blood alcohol concentration. The vertical axis of the graph represents the alcohol area value for 180 minutes after alcohol loading. FIG. 8 is a diagram showing the concentration of acetaldehyde in human blood. The vertical axis of the graph indicates blood acetaldehyde concentration (mg / L), and the horizontal axis of the graph indicates time (minutes) after ethanol loading. It is a figure which shows the acetaldehyde area value for 180 minutes obtained from the measurement result of the human blood acetaldehyde density | concentration. The vertical axis of the graph represents the acetaldehyde area value for 180 minutes after alcohol loading.

One aspect of the present invention is a composition for suppressing alcohol absorption, which contains a heat-treated product of plant-derived peptides as an active ingredient.

1. Plant-derived peptide In the present specification, unless otherwise specified, “plant-derived peptide” refers to a plant-derived protein or a plant containing a protein known in the degradation treatment (decomposition treatment by heat or pressure, degradation treatment by acid or alkali). It means a peptide produced by subjecting it to a low molecular weight by subjecting it to an enzymatic degradation treatment or the like.

The plant-derived peptide may be one type of peptide obtained from a plant-derived protein or a plant containing the protein, or may be a mixture of two or more types of peptides. The number of amino acids constituting the plant-derived peptide is not particularly limited, but is preferably 2 to several tens, more preferably 2 to several (that is, oligopeptide).

In the present invention, it is preferable to use a plant-derived peptide having a high proportion of peptides having a molecular weight of 5000 or less, more preferably a peptide having a high proportion of peptides having a molecular weight of 3000 or less, and a high proportion of peptides having a molecular weight of 1000 or less. It is particularly preferable to use one. Here, “the ratio of the peptide is high” means a state in which at least 50% of the whole plant-derived peptide corresponds to the peptide. The molecular weight can be measured using a method and apparatus (such as HPLC) well known to those skilled in the art.

Plant-derived peptides are not particularly limited, but plant-derived peptides such as seeds, leaves, beans, or moss can be used. Examples of seeds include barley, wheat (including wheat germ), malt, sesame seeds, rice, and coffee. Examples of the leaves include tea (green tea, black tea, oolong tea) and the like. Examples of beans include soybeans, red beans, and black beans. Examples of moss include sweet potatoes and potatoes. Among these plants, malt, tea, and soybean are preferably used in the present invention. Among the teas, green tea is preferably used. In addition, in this specification, description of "derived" may be abbreviate | omitted about the plant origin peptide derived from a specific plant. For example, in the case of a peptide derived from malt, this may be referred to as a malt peptide. At this time, both are used interchangeably.

Plant-derived peptides can be obtained by decomposing plant-derived proteins or plant bodies containing proteins by a conventionally known method. Examples of such decomposition treatment include decomposition treatment with heat or pressure, decomposition treatment with acid or alkali, and decomposition treatment with an enzyme. In any treatment, water, ethanol or the like can be used as a solvent. For example, in the case of a decomposition treatment by heating, a condition of 30 minutes to several hours at a temperature of 100 ° C. or higher is indicated. In addition, this heat processing can also be performed simultaneously with the heat processing of the plant-derived peptide heat-processed material mentioned above. Moreover, if it is a decomposition process by an enzyme, various proteolytic enzymes (protease) can be used suitably according to the objective. As the plant-derived peptide in the present invention, one prepared by itself using a method known per se may be used, or a commercially available product may be used.

2. Plant-derived peptide heat-treated product A plant-derived peptide heat-treated product is obtained by heat-treating a plant-derived peptide in a liquid at high temperature. Such heat treatment is not particularly limited, but is preferably performed after adding not only high temperature conditions but also high pressure conditions. Therefore, the heat-treated plant-derived peptide in the present invention is preferably a high-temperature and high-pressure treated product of a plant-derived peptide (more specifically, a high-temperature and high-pressure treated product in a liquid of a plant-derived peptide).

As used herein, “high temperature and high pressure” means a temperature of 100 ° C. or higher and a pressure exceeding atmospheric pressure. The temperature is preferably 105 ° C. or higher, 110 ° C. or higher, 115 ° C. or higher, 120 ° C. or higher, 125 ° C. or higher, 130 ° C. or higher, or 135 ° C. or higher. The temperature is preferably 170 ° C. or lower, 165 ° C. or lower, 160 ° C. or lower, 155 ° C. or lower, 150 ° C. or lower, 145 ° C. or lower, or 140 ° C. or lower. In addition, this temperature shows the value which measured the exit temperature of the extraction column, when using a pressure-resistant extraction apparatus as a heating apparatus, and when using an autoclave as a heating apparatus, it is the temperature of the center temperature in a pressure vessel. The measured value is shown.

Regarding the pressure under the high temperature and high pressure conditions, the numerical value is not particularly limited as long as the pressure exceeds atmospheric pressure, but preferably 0.101 MPa or more, 0.15 MPa or more, 0.2 MPa or more, 0.25 MPa or more, or 0 .3 MPa or more. The pressure is preferably 0.79 MPa or less, 0.75 MPa or less, 0.7 MPa or less, 0.65 MPa or less, 0.6 MPa or less, 0.55 MPa or less, 0.5 MPa or less, or 0.48 MPa or less. .

The treatment time for obtaining the plant-derived peptide heat-treated product is not particularly limited as long as a treated product exhibiting the effects of the present invention is obtained. The treatment time is, for example, about 15 minutes to 600 minutes, preferably about 30 minutes to 500 minutes, and more preferably about 60 minutes to 300 minutes. In the present invention, a more suitable heat treatment condition for obtaining a plant-derived heat-treated peptide is, for example, the following coordinate system in a coordinate system in which the horizontal axis is time (min.) And the vertical axis is temperature (° C.). It is a heat treatment that is maintained within a range of time and temperature surrounded by (i) to (vi). (i) (170 ° C, 30 min.), (ii) (150 ° C, 30 min.), (iii) (115 ° C, 180 min.), (iv) (105 ° C, 480 min.), (v ) (135 ℃, 480 min.), (Vi) (150 ℃, 180 min.)

Although the treatment conditions regarding the temperature, pressure, and time of the plant-derived peptide for obtaining the heat-treated product of the plant-derived peptide are not particularly limited, for example, they can be set as follows:
(Temperature, pressure, time) =
(105 ° C to 170 ° C, 0.101 MPa to 0.79 MPa, 15 minutes to 600 minutes),
(105 ° C to 170 ° C, 0.101 MPa to 0.79 MPa, 30 minutes to 500 minutes),
(105 ° C to 170 ° C, 0.101 MPa to 0.79 MPa, 60 minutes to 300 minutes),
(105 ° C to 170 ° C, 0.15 MPa to 0.48 MPa, 15 minutes to 600 minutes),
(105 ° C to 170 ° C, 0.15 MPa to 0.48 MPa, 30 minutes to 500 minutes),
(105 ° C to 170 ° C, 0.15 MPa to 0.48 MPa, 60 minutes to 300 minutes),
(110 ° C. to 150 ° C., 0.101 MPa to 0.79 MPa, 15 minutes to 600 minutes),
(110 ° C. to 150 ° C., 0.101 MPa to 0.79 MPa, 30 minutes to 500 minutes),
(110 ° C. to 150 ° C., 0.101 MPa to 0.79 MPa, 60 minutes to 300 minutes),
(110 ° C. to 150 ° C., 0.15 MPa to 0.48 MPa, 15 minutes to 600 minutes),
(110 ° C. to 150 ° C., 0.15 MPa to 0.48 MPa, 30 minutes to 500 minutes),
(110 ° C. to 150 ° C., 0.15 MPa to 0.48 MPa, 60 minutes to 300 minutes),
(120 ° C. to 140 ° C., 0.101 MPa to 0.79 MPa, 15 minutes to 600 minutes),
(120 ° C. to 140 ° C., 0.101 MPa to 0.79 MPa, 30 minutes to 500 minutes),
(120 ° C. to 140 ° C., 0.101 MPa to 0.79 MPa, 60 minutes to 300 minutes),
(120 ° C. to 140 ° C., 0.15 MPa to 0.48 MPa, 15 minutes to 600 minutes),
(120 ° C. to 140 ° C., 0.15 MPa to 0.48 MPa, 30 minutes to 500 minutes),
(120 ° C. to 140 ° C., 0.15 MPa to 0.48 MPa, 60 minutes to 300 minutes) and the like.

In the present invention, the plant-derived peptide heat-treated product can be prepared using an apparatus that can provide the above-described treatment conditions. Examples of such a device include, but are not limited to, a pressure-resistant extraction device, a pressure cooker, and an autoclave that are well known to those skilled in the art. Moreover, the plant-derived peptide heat-treated product may have been subjected to solid-liquid separation before and / or after the heat treatment. By performing the solid-liquid separation process, the liquid part can be recovered, and it can be handled only by the solid. For solid-liquid separation, means such as filtration and / or centrifugation are used. Moreover, the plant-derived peptide heat-treated product may be subjected to a purification treatment after the heat treatment. By performing the purification treatment, specific components contained in the heat-treated peptide derived from a plant can be concentrated. The purification treatment of the plant-derived peptide heat-treated product can be performed using a method and apparatus known per se. Moreover, the plant-derived peptide heat-treated product may have been further clarified before and / or after the heat treatment. The clarification treatment can be performed using a method and apparatus known per se, and the treatment can increase the degree of freedom in designing food and drink to which the plant-derived peptide heat-treated product is added. The plant-derived peptide heat-treated product may be freeze-dried or powdered using a method and apparatus known per se. In addition, the manufacturing method of the plant-derived peptide heat-processed material in this invention can refer to the method of international publication 2014/200000.

3. The cyclic dipeptide or its salt heat-treated peptide derived from a plant can be obtained by heat-treating a plant-derived peptide. At this time, a large amount of the cyclic dipeptide or its salt is included in the heat-treated product derived from a plant peptide. become. That is, in the present invention, the plant-derived heat-treated peptide contains a cyclic dipeptide or a salt thereof as one of its characteristics. Although not wishing to be bound by any particular theory, since the plant-derived peptide heat-treated product contains a large amount of cyclic dipeptide or a salt thereof, the cyclic dipeptide or a salt thereof is effective for the composition for suppressing alcohol absorption. Can be an ingredient. In the present specification, a cyclic dipeptide or a salt thereof may be simply referred to as a cyclic dipeptide.

As used herein, a cyclic dipeptide refers to a dipeptide having a diketopiperazine structure produced by dehydration condensation of an amino group and a carboxyl group of an amino acid. Therefore, the cyclic dipeptide is distinguished from the chain dipeptide.

The cyclic dipeptide includes any combination of amino acids, and the two types of amino acids may be the same or different. Although it does not specifically limit as a kind of cyclic dipeptide, For example, a cycloalanyl glutamine (CAS | Registry | Number: 268221-76-7; Cyclo (Ala-Gln)), cyclo histidyl proline (CAS | Registry | Number: 53109-32) -3; Cyclo (His-Pro)), cycloalanylalanine (CAS Registry Number: 5845-61-4; Cyclo (Ala-Ala)), cycloglycylproline (CAS Registry Number: 3705-27-9; Cyclo (Gly-Pro)), cycloseryltyrosine (CAS Registry Number: 21754-31-4; Cyclo (Ser-Tyr)), cycloprolylthreonine (CAS Registry Number: 227777-31-3; Cyclo (Pro-Thr) ), Cyclohistidylphenylalanine (CAS Registry Number: 56586-95-9; Cyclo (His-Phe)), cycloalanylproline (CAS Registry Number: 65556-33-4; Cyclo (Ala-Pro)), cyclo Phenylalanylserine (CAS Registry Number: 35591-00-5; Cyclo (Phe-Ser)), cycloglycylleucine (CAS Registry Number: 5845) -67-0; Cyclo (Gly-Leu)), cycloglycylphenylalanine (CAS Registry Number: 10125-07-2; Cyclo (Gly-Phe)), cycloprolylproline (Cyclo (Pro-Pro)), cyclo Glycyltryptophan (Cyclo (Gly-Trp)), cycloaspartylphenylalanine (CAS Registry Number: 5262-10-2; Cyclo (Asp-Phe)), cyclovalylproline (Cyclo (Val-Pro)), cycloprolyl Tyrosine (Cyclo (Pro-Tyr)), Cyclomethionylproline (Cyclo (Met-Pro)), Cyclomethionylmethionine (Cyclo (Met-Met)), Cyclovalylvaline (Cyclo (Val-Val)), Cycloleu Sylproline (CAS Registry Number: 2873-36-1; Cyclo (Leu-Pro)), cyclotryptophanyltyrosine (Cyclo (Trp-Tyr)), cyclophenylalanylproline (CAS Registry Number: 3705-26-8) Cyclo (Phe-Pro)), cycloleucyltryptophan (CAS Registry Number: 15136-34-2; Cyclo (Leu-Trp)), Cyclophenylalanyltryptophan (CAS Registry Number: 82597-82-8; Cyclo (Phe-Trp)), cycloleucylphenylalanine (CAS Registry Number: 7280-77-5; Cyclo (Leu-Phe)), cycloleucyl Examples include leucine (CAS Registry Number: 952-45-4; Cyclo (Leu-Leu)), cyclophenylalanylphenylalanine (CAS51Registry Number: 2862-51-3; Cyclo (Phe-Phe)), and the like. The cyclic dipeptide may be only one type or a combination of two or more types. As for cyclic dipeptides, if the two types of amino acids have the same structure, any order may be used. For example, [Cyclo (Pro-Hyp)] and [Cyclo (Hyp-Pro)] are It represents the same cyclic dipeptide.

Cyclic dipeptide salts include any pharmacologically acceptable salt (including inorganic and organic salts). Examples of such salts include sodium salt, potassium salt, calcium salt, magnesium salt, ammonium salt, hydrochloride, sulfate, nitrate, phosphate, organic acid salt (acetate, citrate, maleate, Malate, oxalate, lactate, succinate, fumarate, propionate, formate, benzoate, picrate, benzenesulfonate, etc.), but not limited thereto .

The amount of cyclic dipeptide or a salt thereof contained in the heat-treated peptide derived from a plant varies depending on the kind of the heat-treated peptide derived from a peptide and is not particularly limited. For example, the content is Cyclo (Ala-Gln), Cyclo (Ala-Ala), Cyclo (Ser-Tyr), Cyclo (Gly-Trp), Cyclo (Val-Val), Cyclo (Trp-Tyr), Cyclo Any one or more of (Leu-Trp) and Cyclo (Phe-Phe) is 10 μg / 100 g / Bx or more as the content per Brix (Bx). As another example, the total amount of the cyclic dipeptide or a salt thereof contained in the heat-treated product derived from a plant is preferably 20000 μg / 100 g / Bx or more, 25000 μg / 100 g / Bx or more, 30000 μg / 100 g / Bx or more, more preferably 35000 μg. / 100 g / Bx or more. The total amount is preferably 10,000 mg / 100 g / Bx or less, 5000 mg / 100 g / Bx or less, 2000 mg / 100 g / Bx or less, more preferably 1000 mg / 100 g / Bx or less. Typically, the total amount of the cyclic dipeptide or a salt thereof contained in the heat-treated product of the plant-derived peptide in the present invention is preferably 20000 μg / 100 g / Bx to 10,000 mg / 100 g / Bx, more preferably 30000 μg / 100 g / Bx to 5000 mg / 5000. 100 g / Bx, even more preferably 35000 μg / 100 g / Bx to 1000 mg / 100 g / Bx. When the cyclic dipeptide is in the form of a salt, the content is calculated after conversion to a free form (free form). In the present specification, unless otherwise specified, the total amount of the cyclic dipeptide or a salt thereof represents the total value of any cyclic dipeptide represented by Cyclo (X-Y). However, X and Y are glycine, alanine, valine, leucine, isoleucine, serine, threonine, aspartic acid, glutamic acid, asparagine, glutamine, arginine, lysine, cysteine, methionine, phenylalanine, tyrosine, tryptophan, histidine, proline, hydroxyproline, etc. Any amino acid selected from the group consisting of: X and Y may be the same or different. In this specification, “content per Brix (Bx)” means an amount determined by a value corresponding to a mass percentage of a sucrose solution at 20 ° C. (an aqueous solution containing only sucrose as a solute). Unless otherwise specified, “ppm” used in the present specification means ppm of weight / volume (w / v), and 1.0 ppm / Brix is 0.1 mg / wt when the specific gravity of the solvent is 1. Converted to mL and converted to 0.01% by weight. The content of the cyclic dipeptide or its salt per Bx can be measured with a commercially available Bx measuring instrument.

Favorable aspects of the plant-derived peptide heat-treated product in the present invention include tea peptide heat-treated products, soybean peptide heat-treated products, and malt peptide heat-treated products. Hereinafter, the cyclic dipeptide in these heat-treated products will be described in detail.

(Tea peptide heat-treated product)
As tea used as an extraction raw material, tea leaves (scientific name: Camellia sinensis) manufactured tea leaves, stems, and other parts that can be extracted and consumed can be used. Also, the form is not limited to large leaves or powders. The tea harvest period can also be appropriately selected according to the desired flavor.

The heat-treated tea peptide product of the present invention is characterized in that it is produced without undergoing a fermentation process, thereby suppressing the production of by-products and obtaining a savory product. From this flavor point of view, the tea is made from steamed non-fermented tea (green tea) such as Sencha, Bancha, Hojicha, Gyokuro, Kabusecha, Tsujicha, Ureshino tea, Aoyagi tea, various Chinese tea, etc. It is preferable to use non-fermented tea.

The present inventors have measured the concentration of cyclic dipeptide in an extract obtained from commercially available tea. As a result, it has been confirmed that a very small amount (about 0 to 200 μg / 100 g / Bx) of fermented tea is contained, and that green tea is hardly contained.

On the other hand, the heat-treated tea peptide in the present invention is a cyclic dipeptide Cyclo (Ala-Gln), Cyclo (Ala-Ala), Cyclo (Ser-Tyr), Cyclo (Gly) that was not included in conventional teas. -Trp), Cyclo (Val-Val), Cyclo (Trp-Tyr), Cyclo (Leu-Trp) and Cyclo (Phe-Phe) are contained at a concentration of 10 μg / 100 g / Bx or more.

The heat-treated tea peptide in the present invention is Cyclo (Ala-Gln), Cyclo (His-Pro), Cyclo (Ala-Ala), Cyclo (Gly-Pro), Cyclo (Ser-Tyr), Cyclo (Pro- Thr), Cyclo (His-Phe), Cyclo (Ala-Pro), Cyclo (Phe-Ser), Cyclo (Gly-Leu), Cyclo (Gly-Phe), Cyclo (Pro-Pro), Cyclo (Asp-Phe ), Cyclo (Val-Pro), Cyclo (Pro-Tyr), Cyclo (Met-Pro), Cyclo (Leu-Pro), Cyclo (Phe-Pro), Cyclo (Leu-Phe), and Cyclo (Leu-Leu) ) At a concentration of 0.1 ppm / Bx (10 μg / 100 g / Bx) or more. Preferably, each of the above cyclic dipeptides contains 0.2 ppm / Bx or more, more preferably 0.3 ppm / Bx or more, still more preferably 0.4 ppm / Bx or more, particularly preferably 0.5 ppm / Bx or more. It is a peptide heat-treated product. Furthermore, Cyclo (Gly-Trp), Cyclo (Val-Val), Cyclo (Trp-Tyr), Cyclo (Leu-Trp), Cyclo (Phe-Trp) and Cyclo (Phe-Phe) are each 0.1 ppm / Bx (10 μg / 100 g / Bx) or more, preferably 0.2 ppm / Bx or more, more preferably 0.3 ppm / Bx or more.

Cyclic dipeptides with strong bitterness include Cyclo (Leu-Pro) and Cyclo (Phe-Pro) which are cyclic dipeptides in coffee beverages (see JP 2010-166911 A), and Cyclo (Leu-) which is a degradation product of casein. Trp) (Protein Research Institute Peptide Institute Bulletin, No. 2, 1974) is known. Although the tea peptide heat-treated product in the present invention contains these cyclic dipeptides having a strong bitter taste, the heat-treated product itself has almost no bitter taste. When an aqueous solution containing Cyclo (Leu-Pro), Cyclo (Phe-Pro) and Cyclo (Leu-Trp) at the same concentration as the heat-treated tea peptide was prepared, a strong bitter taste was felt and it coexists. Other cyclic dipeptides and tea-derived components are thought to additively or synergistically reduce the bitter taste of Cyclo (Leu-Pro), Cyclo (Phe-Pro) and Cyclo (Leu-Trp). In particular, the total amount of cyclic dipeptides Cyclo (Leu-Pro), Cyclo (Phe-Pro) and Cyclo (Leu-Trp) having a bitter taste relative to the total amount (A) of Cyclo (Leu-Leu) and Cyclo (Leu-Phe) (B ) Ratio [(B) / (A)] is 1.0 or less (preferably 0.8 or less, more preferably 0.6 or less, particularly preferably 0.4 or less), It is a cyclic dipeptide-containing heat-treated product with no taste such as bitterness, and can be applied orally.

The total amount of cyclic dipeptide in the heat-treated tea peptide in the present invention is preferably 20000 μg / 100 g / Bx or more, 25000 μg / 100 g / Bx or more, 30000 μg / 100 g / Bx or more, more preferably 35000 μg / 100 g / Bx or more. The total amount is preferably 10,000 mg / 100 g / Bx or less, 5000 mg / 100 g / Bx or less, 2000 mg / 100 g / Bx or less, more preferably 1000 mg / 100 g / Bx or less. Typically, the total amount of the cyclic dipeptide or a salt thereof contained in the heat-treated tea peptide in the present invention is preferably 20000 μg / 100 g / Bx to 10000 mg / 100 g / Bx, more preferably 30000 μg / 100 g / Bx to 5000 mg / 100 g. / Bx, more preferably 35000 μg / 100 g / Bx to 1000 mg / 100 g / Bx.

The tea peptide heat-treated product in the present invention preferably contains Cyclo (Leu-Leu), Cyclo (Leu-Phe), and Cyclo (Ala-Ala) at a high concentration. Specifically, Cyclo (Leu-Leu) is 10% or more (weight basis), Cyclo (Leu-Phe) is 10% or more, and Cyclo (Ala-Ala) with respect to the total amount of cyclic dipeptide in the heat-treated tea peptide. Is a heat-treated product with 7% or more. When these are expressed in terms of concentration, it becomes a heat-treated tea peptide product having a concentration of 5.0 ppm / Bx (500 μg / 100 g / Bx) or more, preferably 8.0 ppm / Bx or more, more preferably 10.0 ppm / Bx or more. . These upper limits are 50.0 ppm / Bx or less, preferably 40.0 ppm / Bx or less, more preferably 35.0 ppm / Bx or less, and further preferably about 30.0 ppm / Bx or less.

In another preferred embodiment, the heat-treated tea peptide product of the present invention contains Cyclo (Leu-Leu), Cyclo (Leu-Phe), and Cyclo (Phe-Phe) at a high concentration.

By the way, it is known that a cyclic dipeptide having a hydrophobic functional group is more hydrophobic than a linear peptide by cyclization. Cyclo (Phe-Phe) is the most hydrophobic component, but as a result of accelerated storage test (55 ° C, 2 weeks) of the above-mentioned heat-treated tea peptide, Cyclo (Phe-Phe) is stably maintained. I have confirmed that. Therefore, the heat-treated tea peptide in the present invention is also useful as a heat-treated product containing Cyclo (Phe-Phe). The Cyclo (Phe-Phe) content in the heat-treated tea peptide is preferably 10 μg / 100 g / Bx or more, 20 μg / 100 g / Bx or more, 30 μg / 100 g / Bx or more.

(Heat-treated soybean peptide)
Soybeans (scientific name: Glycine max) used as a raw material can be used without restriction of varieties and production areas, and can also be used in processed products such as pulverized products. It is said that protein in soybeans accounts for about 30%. Soy protein does not have much water-insoluble protein like tea protein, so pretreatment for removing water-soluble protein is not essential and may be performed as necessary. When there is no pretreatment for removing the water-soluble protein, a soybean peptide heat-treated product containing a cyclic dipeptide at a high concentration can be more easily produced by a one-pot reaction.

In the present invention, the soybean peptide heat-treated product is a cyclic dipeptide that was not included in the conventional soybean protein degradation product (soy peptide), Cyclo (Ala-Gln), Cyclo (Ala-Ala), Cyclo (Ser-Tyr) , Cyclo (Gly-Trp), Cyclo (Val-Val), Cyclo (Trp-Tyr), Cyclo (Leu-Trp) and Cyclo (Phe-Phe) content of 10 μg / 100 g / Bx or more Contains.

The heat-treated soybean peptide in the present invention is Cyclo (Ala-Gln), Cyclo (His-Pro), Cyclo (Ala-Ala), Cyclo (Gly-Pro), Cyclo (Ser-Tyr), Cyclo (Pro- Thr), Cyclo (His-Phe), Cyclo (Ala-Pro), Cyclo (Phe-Ser), Cyclo (Gly-Leu), Cyclo (Gly-Phe), Cyclo (Gly-Trp), Cyclo (Asp-Phe ), Cyclo (Val-Pro), Cyclo (Pro-Tyr), Cyclo (Met-Pro), Cyclo (Val-Val), Cyclo (Leu-Pro), Cyclo (Trp-Tyr), Cyclo (Phe-Pro) , Cyclo (Leu-Trp), Cyclo (Leu-Phe), Cyclo (Leu-Leu) and Cyclo (Phe-Phe) are each contained at a concentration of 0.1 ppm / Bx (10 μg / 100 g / Bx). Preferably, each cyclic dipeptide is 0.5 ppm / Bx or more, more preferably 0.7 ppm / Bx or more, still more preferably 0.9 ppm / Bx or more, particularly preferably 1.0 ppm / Bx or more, and particularly preferably 1. It is a soybean peptide heat-treated product containing at a concentration of 2 ppm / Bx or more. Furthermore, Cyclo (Pro-Pro) and Cyclo (Phe-Trp) are each 0.1 ppm / Bx (10 μg / 100 g / Bx) or more, preferably 0.2 ppm / Bx or more, more preferably 0.3 ppm / Bx or more. It can be made to contain in the density | concentration.

This heat-treated product of soybean peptide (especially heat-treated product obtained from soybean or a pulverized product thereof) is known as Cyclo (Leu-Pro), Cyclo (Phe-Pro) and Cyclo (Leu Despite containing -Trp), its bitterness is reduced. When an aqueous solution containing the same concentration of Cyclo (Leu-Pro) and Cyclo (Phe-Pro) was prepared, a strong bitter taste was felt, so other cyclic dipeptides and components derived from soybeans were added together. It is considered that the bitter taste of Cyclo (Leu-Pro), and Cyclo (Phe-Pro) and Cyclo (Leu-Trp) is moderately or synergistically. In particular, the total amount of cyclic dipeptides Cyclo (Leu-Pro), Cyclo (Phe-Pro) and Cyclo (Leu-Trp) having a bitter taste relative to the total amount (A) of Cyclo (Leu-Leu) and Cyclo (Leu-Phe) (B ) Ratio [(B) / (A)] is 1.0 or less (preferably 0.8 or less, more preferably 0.6 or less, particularly preferably 0.5 or less), It is a cyclic dipeptide-containing heat-treated product with significantly reduced bitterness and can be applied orally.

The total amount of cyclic dipeptide in the soybean peptide heat-treated product in the present invention is preferably 20000 μg / 100 g / Bx or more, 25000 μg / 100 g / Bx or more, 30000 μg / 100 g / Bx or more, more preferably 35000 μg / 100 g / Bx or more. The total amount is preferably 10,000 mg / 100 g / Bx or less, 5000 mg / 100 g / Bx or less, 2000 mg / 100 g / Bx or less, more preferably 1000 mg / 100 g / Bx or less. Typically, the total amount of the cyclic dipeptide or a salt thereof contained in the heat-treated soybean peptide in the present invention is preferably 20000 μg / 100 g / Bx to 10,000 mg / 100 g / Bx, more preferably 30000 μg / 100 g / Bx to 5000 mg / 100 g. / Bx, more preferably 35000 μg / 100 g / Bx to 1000 mg / 100 g / Bx.

The soybean peptide heat-treated product in the present invention preferably contains Cyclo (Leu-Leu), Cyclo (Leu-Phe), Cyclo (Ser-Tyr) and Cyclo (Pro-Thr) in a high concentration. Specifically, Cyclo (Leu-Leu) is 8% or more (weight basis), Cyclo (Leu-Phe) is 8% or more, and Cyclo (Ser-Tyr) based on the total amount of cyclic dipeptide in the soybean peptide heat-treated product. Is a heat-treated product with 6% or more. In the soybean peptide heat-treated product, these concentrations are each 5.0 ppm / Bx (500 μg / 100 g / Bx) or more, preferably 6.0 ppm / Bx or more, more preferably 7.0 ppm / Bx or more. In particular, the content of Cyclo (Leu-Leu) and Cyclo (Leu-Phe) is 10.0 ppm / Bx or more, preferably 12.0 ppm / Bx or more. These upper limits are 50.0 ppm / Bx or less, preferably 40.0 ppm / Bx or less, more preferably 35.0 ppm / Bx or less, and further preferably about 30.0 ppm / Bx or less.

(Heat-treated malt peptide)
The malt used as a raw material can be used without limitation of the variety and the production area, but barley malt obtained by germinating barley seeds is particularly preferably used. For barley malt, it is practical and efficient to remove the skin part and separate and use a fraction having a high protein content. As for the fraction having a high protein content, there is a method in which malt is gradually shaved from the surface, the husk is removed, and then a fraction containing a large amount of protein such as an aleurone layer and endosperm is shaved off. Alternatively, an extraction residue can be used, and examples of the extraction residue include malt squeezed rice cake produced during beer production. In the present specification, barley malt may be simply referred to as malt.

If a fraction having a high protein content is used as a raw material, a malt peptide heat-treated product containing a cyclic dipeptide at a high concentration can be more easily produced by a one-pot reaction.

The malt peptide heat-treated product in the present invention is a cyclic dipeptide that has conventionally been difficult to extract, Cyclo (Ala-Gln), Cyclo (Ala-Ala), Cyclo (Ser-Tyr), Cyclo (Gly-Trp), Cyclo (Val -Val), Cyclo (Trp-Tyr), Cyclo (Leu-Trp) and Cyclo (Phe-Phe) are contained at a concentration of 10 μg / 100 g / Bx or more.

Further, the heat-treated malt peptide in the present invention is Cyclo (Ala-Gln), Cyclo (His-Pro), Cyclo (Ala-Ala), CycloC (Gly-Pro), Cyclo (Ser-Tyr), Cyclo (Pro- Thr), Cyclo (His-Phe), Cyclo (Ala-Pro), Cyclo (Phe-Ser), Cyclo (Gly-Leu), Cyclo (Gly-Phe), Cyclo (Gly-Trp), Cyclo (Asp-Phe ), Cyclo (Val-Pro), Cyclo (Pro-Tyr), Cyclo (Met-Pro), Cyclo (Val-Val), Cyclo (Leu-Pro), Cyclo (Trp-Tyr), Cyclo (Phe-Pro) , Cyclo (Leu-Trp), Cyclo (Leu-Phe), Cyclo (Leu-Leu) and Cyclo (Phe-Phe) are each contained at a concentration of 0.1 ppm / Bx (50 μg / 100 g / Bx) or more. The malt peptide heat-treated product preferably contains 0.3 ppm / Bx or more, more preferably 0.4 ppm / Bx or more, still more preferably 0.5 ppm / Bx or more, particularly preferably 0.6 ppm / Bx or more of each of the above cyclic dipeptides. Containing at a concentration of

Although the malt peptide heat-treated product in the present invention contains Cyclo (Leu-Pro), Cyclo (Phe-Pro), and Cyclo (Leu-Trp), which are known as cyclic dipeptides having a strong bitter taste, Has been reduced. In particular, the total amount of cyclic dipeptides Cyclo (Leu-Pro), Cyclo (Phe-Pro) and Cyclo (Leu-Trp) having a bitter taste relative to the total amount (A) of Cyclo (Leu-Leu) and Cyclo (Leu-Phe) (B ) Ratio [(B) / (A)] is 1.0 or less (preferably 0.8 or less) malt peptide heat-treated product, which is a cyclic dipeptide-containing heat-treated product with significantly reduced bitterness. Applicable.

The total amount of the cyclic dipeptide in the heat-treated malt peptide in the present invention is preferably 20000 μg / 100 g / Bx or more, 25000 μg / 100 g / Bx or more, 30000 μg / 100 g / Bx or more, more preferably 35000 μg / 100 g / Bx or more. The total amount is preferably 10,000 mg / 100 g / Bx or less, 5000 mg / 100 g / Bx or less, 2000 mg / 100 g / Bx or less, more preferably 1000 mg / 100 g / Bx or less. Typically, the total amount of the cyclic dipeptide or a salt thereof contained in the heat-treated malt peptide in the present invention is preferably 20000 μg / 100 g / Bx to 10,000 mg / 100 g / Bx, more preferably 30000 μg / 100 g / Bx to 5000 mg / 100 g. / Bx, more preferably 35000 μg / 100 g / Bx to 1000 mg / 100 g / Bx.

The malt peptide heat-treated product in the present invention preferably contains Cyclo (Leu-Leu), Cyclo (Leu-Phe) and Cyclo (Ala-Ala) at a high concentration. Specifically, these concentrations are 5.0 ppm / Bx (500 μg / 100 g / Bx) or more, preferably 6.0 ppm / Bx or more, more preferably 7.0 ppm / Bx or more, respectively, in the malt peptide heat-treated product. These upper limits are 50.0 ppm / Bx or less, preferably 40.0 ppm / Bx or less, more preferably 30.0 ppm / Bx or less, and further preferably about 20.0 ppm / Bx or less.

4). Composition for suppressing alcohol absorption In the present invention, the “composition for suppressing alcohol absorption” means a composition for suppressing absorption of alcohol taken from outside the body into the body. Since the composition of the present invention only needs to suppress the absorption of alcohol into the body as a result, it can also be referred to as an “alcohol absorption inhibiting composition” as the term. Inhibition of alcohol absorption and promotion of alcohol metabolism are mutually different technical ideas. The former is an action at a relatively early stage after alcohol intake, while the latter is once absorbed after alcohol intake. This is the action of subsequently decomposing (disappearing) the alcohol. The present invention is a composition specialized in the alcohol absorption inhibiting action among these.

The content of the plant-derived heat-treated peptide in the composition of the present invention is not particularly limited as long as the desired effect of the present invention can be obtained in consideration of its administration form, administration method, and the like. It is not a thing. For example, the content of the plant-derived peptide heat-treated product is 0.02% by weight or more, preferably 0.2% by weight or more, more preferably 2% by weight or more with respect to the total weight of the composition of the present invention. The content of the plant-derived peptide heat-treated product is 99% by weight or less, preferably 50% by weight or less, more preferably 10% by weight or less based on the total weight of the composition of the present invention. As used herein, “% by weight” means weight / weight (w / w) unless otherwise specified. In addition, the weight of the heat-treated product of plant-derived peptide can be defined by the weight of the heat-treated product of plant-derived peptide that has been powdered. When a cyclic dipeptide or a salt thereof is used as an active ingredient, the amount contained in the heat-treated peptide derived from a plant can correspond to the above content.

The alcohol absorption inhibiting composition of the present invention can be provided in the form of an agent as an example, but is not limited to this form. The agent can be provided as a composition as it is or as a composition containing the agent. Examples of the composition of the present invention include, but are not limited to, a pharmaceutical composition, a food / beverage product composition, a food composition, a beverage composition, a cosmetic composition, and the like. Non-limiting examples of food compositions include functional foods, health supplements, functional nutrition foods, special foods, foods for specified health use, dietary supplements, diet foods, health foods, supplements, food additives, etc. Can be mentioned.

The composition of the present invention, for example, by adding a solvent, a dispersant, an emulsifier, a buffer, a stabilizer, an excipient, a binder, a disintegrant, or a lubricant, etc., as necessary, to the plant-derived peptide heat-treated product, According to a known method, it can be formulated into a solid preparation such as a tablet, granule, powder, powder, or capsule, or a liquid preparation such as a normal solution, suspension, or emulsion. These compositions can be taken with water or the like as it is. Moreover, after preparing the form (for example, powder form and granule form) which can be mix | blended easily, it can use, for example as a raw material of a pharmaceutical.

5). Application The composition of the present invention can effectively suppress an increase in the concentration of alcohol in the body (particularly blood alcohol) by suppressing the absorption of alcohol into the body. Therefore, the composition of the present invention is useful as a composition for suppressing an increase in the concentration of body alcohol (particularly blood alcohol). In the present specification, “blood alcohol concentration” means the ratio of alcohol contained in 100 mL of blood expressed in% (w / v), and the measurement can be performed using a commercially available device or kit. it can.

The composition of the present invention can also be used as a composition for treating or preventing a disease or symptom associated with an increase in blood alcohol concentration because it suppresses the absorption of alcohol into the body. Although it does not specifically limit as a disease or symptom relevant to the raise in blood alcohol level, For example, alcoholism (acute, chronic), alcoholism, fetal alcohol syndrome, alcoholic liver disease (alcoholic fatty liver, alcohol Hepatitis, alcoholic cirrhosis), alcoholic dementia, alcoholic myopathy, alcoholic cardiomyopathy, alcoholic ketoacidosis, alcoholic neuropathy, alcoholic encephalopathy, alcoholic hypoglycemia, alcoholic epilepsy, alcoholic brain atrophy, Examples include alcoholic depression. In the present specification, “treatment or prevention” includes both concepts of making the current state a better state and preventing being worse than the current state. Terms such as improvement, recovery, mitigation, mitigation can also be included in this.

The composition of the present invention can be applied to any therapeutic use (medical use) or non-therapeutic use (non-medical use). Specific examples include use as pharmaceuticals, quasi-drugs, cosmetics, and the like, and those that do not belong to these under the Pharmaceutical Affairs Law, but explicitly or implicitly appeal for alcohol absorption suppression effects. Use.

In another aspect, the present invention relates to the composition for suppressing alcohol absorption, which is labeled with a function exhibited by suppressing the absorption of alcohol. Such indication or functionality indication is not particularly limited, but for example, “suppress alcohol absorption”, “moderate alcohol absorption”, “prevent hangover”, “friendly to the liver”, “ Examples thereof include “protecting the liver”, “maintaining liver function” and the like, or a display or functional display that can be equated with these. In the present specification, indications such as the indication and the functionality indication may be attached to the composition itself, or may be attached to a container or packaging of the composition.

The composition of the present invention can be ingested by an appropriate method according to the form. Examples of the ingestion method include methods for internal use (oral use), external use, injection and the like, but the method is not particularly limited as long as the desired effect of the present invention can be obtained. In addition, in this specification, ingestion is used as what includes all aspects of ingestion, taking, or drinking.

The application amount of the composition of the present invention is set in a timely manner according to the form, application method, purpose of use and age, weight, symptom, etc. of the patient or animal to be applied and is not constant. The effective human intake of the plant-derived peptide heat-treated product in the present invention is not constant, but is, for example, 200 mg or more, preferably 500 mg or more per day for a human body weighing 50 kg. Application to the subject may be performed once or several times within a day within a desired application amount range. The application period is also arbitrary. In addition, the effective human intake of the plant-derived peptide heat-treated product means the intake of the plant-derived peptide heat-treated product exhibiting an effective effect in humans. Moreover, the said intake can be prescribed | regulated by the weight of the powdered plant-derived peptide heat-processed material.

The subject of application of the composition of the present invention is preferably a human, but domestic animals such as cattle, horses and goats, pet animals such as dogs, cats and rabbits, or experiments such as mice, rats, guinea pigs and monkeys. It may be an animal. When applying to animals other than humans, the amount used per day for about 20 g per mouse is the content of active ingredient in the composition, conditions of application target, weight, sex, age, etc. Usually, the amount of heat-treated product derived from a plant is preferably 200 mg / kg or more, preferably 500 mg / kg. The intake amount can be defined by the weight of the powdered plant-derived peptide heat-treated product. When applied to other animals, the application amount of the composition of the present invention may be appropriately adjusted according to the weight or size of the animal.

6). Use of Plant-Derived Peptide Heat Treated Product for Suppressing Alcohol Absorption One aspect of the present invention is the use of plant-derived peptide heat treated product for suppressing alcohol absorption.

The use of the heat-treated peptide derived from the plant of the present invention includes, for example, use for suppressing an increase in body alcohol (particularly blood alcohol) concentration, and treatment or treatment of a disease or symptom associated with an increase in blood alcohol concentration. Use for prevention is included. Although it does not specifically limit as a disease or symptom relevant to the raise in blood alcohol level, For example, alcoholism (acute, chronic), alcoholism, fetal alcohol syndrome, alcoholic liver disease (alcoholic fatty liver, alcohol Hepatitis, alcoholic cirrhosis), alcoholic dementia, alcoholic myopathy, alcoholic cardiomyopathy, alcoholic ketoacidosis, alcoholic neuropathy, alcoholic encephalopathy, alcoholic hypoglycemia, alcoholic epilepsy, alcoholic brain atrophy, Examples include alcoholic depression. The use of the heat-treated product of the plant-derived peptide of the present invention is used in humans or non-human animals, and may be used for therapeutic purposes or non-therapeutic uses. Here, “non-therapeutic” is a concept that does not include a medical act, that is, a treatment act on the human body by treatment.

7). Method for suppressing alcohol absorption One aspect of the present invention is a method for suppressing alcohol absorption, which comprises administering a therapeutically effective amount of a plant-derived peptide heat-treated product as an active ingredient to a subject requiring suppression of alcohol absorption. is there.

It should be noted that the object requiring suppression of alcohol absorption is the same as the application object of alcohol absorption suppression of the present invention.

In addition, in the present specification, the therapeutically effective amount refers to an amount in which alcohol absorption is suppressed when a plant-derived peptide heat-treated product is administered to the subject as compared to a subject not administered. The specific effective amount is appropriately set depending on the administration form, administration method, purpose of use, age, weight, symptom, etc. of the subject and is not constant. The amount by which alcohol absorption is suppressed can be measured or evaluated using the blood alcohol concentration as an index, and the blood alcohol concentration can be measured using a commercially available apparatus or kit as described above. Moreover, suppression of alcohol absorption may be evaluated after examining the activity of an enzyme related to alcohol metabolism. That is, comparing the subject who received the plant-derived heat-treated peptide with the subject not administered, it was determined that the alcohol absorption was suppressed when the activity of the enzyme related to alcohol metabolism did not change between the two subjects. it can.

In the method of the present invention, the plant-derived peptide heat-treated product may be administered as it is, or may be administered as a composition containing a plant-derived peptide heat-treated product so that the therapeutically effective amount is obtained.

According to the method of the present invention, alcohol absorption can be suppressed without causing side effects.

Hereinafter, the present invention will be described in more detail with reference to examples, but the scope of the present invention is not limited thereby. Those skilled in the art can use the method of the present invention with various changes and modifications, and these are also included in the scope of the present invention.

Preparation of heat-treated peptide-derived peptide (1) Heat-treated malt peptide A commercially available malt (barley malt) was used as a plant. It stirred for 30 minutes, keeping water at 45 degreeC with respect to about 50 g of malt. Thereafter, the temperature was raised by 1 ° C. per minute (25 minutes), and when it reached 70 ° C., 100 mL of 70 ° C. water was added. After maintaining at 70 ° C. for 60 minutes, the temperature was lowered to room temperature in 10 to 15 minutes. Thereafter, water was added to make 450 g. This liquid was filtered with a filter paper to prepare a pretreatment of malt. To 100 g of the malt after the pretreatment, 2000 g of hot water was added and stirred as appropriate for extraction for 5 minutes. To 100 g of malt after the water-soluble protein reduction treatment, 0.75 g of enzyme (trade name: Protin NY100, manufactured by Daiwa Kasei Co., Ltd.) and 900 mL of water were added and mixed by shaking at 55 ° C. for 6 hours. Then, this enzyme treatment liquid was heat-treated without solid-liquid separation. The heat treatment was performed at 135 ° C. for 3 hours at high temperature and high pressure in an autoclave (manufactured by Tommy Seiko). The total amount of the cyclic peptide or salt thereof contained in the heat-treated malt peptide thus obtained was 42228.68 μg / 100 g / Brix.

(2) Tea peptide heat-treated product As a plant body, Ichibancha tea leaf (variety: Yabukita, total nitrogen: 6.3%) produced in Kagoshima Prefecture was used. First, the tea was pretreated (pre-extraction three times) to reduce water-soluble protein. That is, 200 g of hot water was added to 10 g of tea, and the mixture was appropriately stirred and extracted for 5 minutes. After the completion of extraction, the mixture was filtered through 140 mesh to recover the extraction residue (tea bowl). To this tea bowl, 200 g of hot water was poured and extracted for 5 minutes to recover the tea bowl. Again, this tea bowl was similarly extracted and the tea bowl was recovered.

Next, the pre-extracted tea (teacup) was subjected to enzyme decomposition treatment. Pour 200g of 50 ° C hot water into the bowl (total amount), add 1g of protease (trade name: Protin NY100, manufactured by Daiwa Kasei Co., Ltd.), and stir with a stir bar (300rpm) in a 55 ° C water bath For 3 hours. Thereafter, the enzyme was inactivated by maintaining at 95 ° C. for 30 minutes.

This enzyme-treated solution was subjected to heat treatment in the form of a tea liquid mixture without solid-liquid separation. The heat treatment was performed in a high temperature high pressure fluid at 135 ° C. for 3 hours in an autoclave (manufactured by Tommy Seiko). The treated liquid was filtered through 140 mesh to obtain a tea peptide heat-treated product (tea extract). The total amount of the cyclic peptide or salt thereof contained in the heat-treated tea peptide thus obtained was 78890.53 μg / 100 g / Brix.

(3) Heat treated soybean peptide A soybean peptide heat treated product was produced by using soybean peptide as a plant-derived peptide and subjecting it to high temperature and high pressure treatment in a liquid. Specifically, 15 g of distilled water was added to 3 g of soy peptide (Hi-New AM, manufactured by Fuji Oil Co., Ltd.), and the mixture was placed in an autoclave (produced by Tommy Seiko Co., Ltd.). High pressure treatment was added. The total amount of the cyclic peptide or salt thereof contained in the heat-treated soybean peptide thus obtained was 91386.43 μg / 100 g / Brix.

Example 1
For male SD rats (7 weeks old), distilled water, malt peptide (enzyme-treated malt in (1) above), heat-treated malt peptide or heat-treated tea peptide at a dose of 1 mL per 100 g body weight Intragastric administration. Immediately thereafter, ethanol (40% w / v) was forcibly administered intragastrically to the rats at a dose of 0.2 g per 100 g body weight. Heparinized blood was collected from the tail vein at 0, 30, 60, 90, 120, and 180 minutes after ethanol administration. The obtained blood was subjected to ethanol measurement using F-kit ethanol (JK International). Moreover, the alcohol area value for 120 minutes was computed from the measurement result.

The results are shown in Fig. 1 and Fig. 2. The measured values of blood ethanol concentration and the alcohol area value for 120 minutes are shown in Tables 1 and 2 below, respectively. By administration of malt peptide, the blood concentration Cmax of ethanol was 0.547 mg / ml, which was lower than that of distilled water administration (Cmax = 0.828 mg / ml). In contrast, administration of the malt peptide heat-treated product resulted in a blood ethanol Cmax of 0.39 mg / ml, further reducing the blood ethanol concentration compared to malt peptide administration. In addition, administration of a heat-treated tea peptide (Cmax = 0.324 mg / ml) also lowered the blood ethanol concentration in the same manner as the heat-treated malt peptide. The alcohol area value for 120 minutes decreased with the administration of heat-treated malt peptide (30.5: 0-120min * mg / ml), and the area value for distilled water (78.4: 0-120min * mg / ml) and malt Compared with peptide administration (44.2: 0-120 min * mg / ml), it was greatly reduced. The heat-treated tea peptide also showed almost the same effect as the heat-treated malt peptide (26.1: 0-120 min * mg / ml).

Example 2
For male SD rats (8 weeks old), distilled water, soy peptide (Hi-Nut AM, manufactured by Fuji Oil Co., Ltd.), or heat-treated product of soy peptide was forcibly administered intragastrically at a dose of 1 mL per 100 g body weight. Immediately thereafter, ethanol (40% w / v) was forcibly administered intragastrically to the rats at a dose of 0.2 g per 100 g body weight. Heparinized blood was collected from the tail vein at 0, 30, 60, 90, 120, and 180 minutes after ethanol administration. The obtained blood was subjected to ethanol measurement using F-kit ethanol (JK International). Moreover, the alcohol area value for 240 minutes was computed from the measurement result.

The results are shown in Fig. 3 and Fig. 4. By administration of soy peptide, the blood concentration Cmax of ethanol became 0.48 mg / ml, which was lower than that of distilled water administration (Cmax = 0.86 mg / ml). On the other hand, administration of the heat-treated soybean peptide resulted in a blood ethanol Cmax of 0.31 mg / ml, which further decreased the blood ethanol concentration compared to administration of the soybean peptide. The alcohol area value for 240 minutes was lowest with administration of heat-treated soybean peptide (48.15: 0-240min * mg / ml), and the area value was administered with distilled water (130.26: 0-240min * mg / ml) and Compared with soy peptide administration (66.7: 0-240 min * mg / ml), it was greatly reduced.

Example 3
For male SD rats (8 weeks old), distilled water and a heat-treated tea peptide were administered by intragastric administration once at a dose of 1 mL per 100 g body weight. In addition, a group in which the same amount of the heat-treated tea peptide was continuously administered for 7 days was provided. Immediately after administration of the heat-treated tea peptide (immediately after the last administration in the case of continuous administration for 7 days), ethanol (40% w / v) is administered by intragastric administration at a dose of 0.2 g per 100 g body weight to the same rat. did. Heparinized blood was collected from the tail vein at 0, 30, 60, 90, and 120 minutes after ethanol administration. The obtained blood was subjected to ethanol measurement using F-kit ethanol (JK International). The alcohol area value for 120 minutes was calculated from the measurement results. In addition, after blood collection at 120 minutes after ethanol administration, anesthesia and blood removal were performed and the liver was removed. After measuring the weight of the extracted liver, the left outer lobe was separated and 50 mg was collected from the center and frozen with liquid nitrogen. The frozen liver tissue was subjected to alcohol metabolizing enzyme (ADH, ALDH) activity measurement. The activity was measured using Alcohol Dehydrogenase Activity Colorimetric Assay Kit (BioVision) for ADH and Aldehyde Dehydrogenase Activity Colorimetric Assay Kit (BioVision) for ALDH.

The result is shown in FIG. By administration of the heat-treated tea peptide product, the blood alcohol area value for 120 minutes was greatly reduced as compared with the administration of distilled water, and continuous administration of the tea peptide heat-treated product for 7 days further reduced the blood alcohol area value. As for ADH and ALDH, no significant increase was confirmed compared with administration of distilled water.

Example 4
Twelve human subjects were divided into two groups, and the intake test of the heat-treated soybean peptide was performed using the crossover method. Specifically, 1000 mg of heat-treated soybean peptide was dissolved in a loaded alcoholic beverage (mixed with 75 ml of 40% whiskey and 75 ml of water) in either stage I or stage II for each group of subjects. I was ingested. In the period when the heat-treated soybean peptide was not ingested, only the loaded alcoholic beverage was ingested. In addition, a washout period of 6 days was provided between the stage I and the stage II, and the soybean peptide heat-treated product was obtained by spray-drying the heat-treated product described in (3) above into a powder form.

The subjects finished dinner by 21:00 on the day before the test, and prohibited eating and drinking other than water after 21:00. On the day of the test, lunch and 330 ml of water were consumed within 15 minutes as meals 120 minutes before alcohol loading. The amount of alcohol loaded was 30 g / human ethanol (150 ml loaded alcoholic beverage / human). Alcohol load was a mixture of 75 ml of 40% whiskey whiskey and 75 ml water, and was consumed within 10 minutes. Blood samples were collected before alcohol loading and at 30, 60, 90, 120, 150, and 180 minutes after loading. Blood was collected 120 minutes after alcohol loading and 100 mL of water was drunk after the completion of various tests. About the blood sample of the obtained subject, blood ethanol concentration and blood acetaldehyde concentration were measured using gas chromatography.

The results of blood ethanol concentration are shown in FIG. 6 and FIG. It was clarified that when the heat-treated soybean peptide was ingested, the blood ethanol concentration was lower than that in the case of not ingesting it. This result suggests that the heat-treated soybean peptide has an alcohol absorption inhibitory action.

On the other hand, the results of blood acetaldehyde concentration are as shown in FIG. 8 and FIG. 9, and the blood acetaldehyde concentration is 30 minutes after alcohol loading when the soy peptide heat-treated product is ingested compared to the case of not ingesting. And it decreased significantly at 90 minutes. This difference is considered that when the heat-treated soybean peptide was ingested as described above, alcohol absorption was suppressed and the blood ethanol concentration was low, so that the concentration of acetaldehyde, which is a metabolite thereof, was also low.

The present invention provides a composition for suppressing alcohol absorption containing a heat-treated product of plant-derived peptides as an active ingredient. Therefore, the present invention can provide an effective and new means that contributes to prevention or treatment of diseases or symptoms associated with an increase in blood alcohol concentration and a disease or symptom associated with an increase in blood alcohol concentration. High availability.

Claims (12)

1. A composition for suppressing alcohol absorption, comprising a plant-derived peptide heat-treated product as an active ingredient.
2. The composition for alcohol absorption suppression according to claim 1, wherein the content of the cyclic dipeptide or the salt thereof in the heat-treated plant-derived peptide is 20000 μg / 100 g / Bx or more.
3. The composition for suppressing alcohol absorption according to claim 1 or 2, wherein the content of the cyclic dipeptide or the salt thereof in the heat-treated peptide derived from a plant is 30000 μg / 100 g / Bx or more.
4. The composition for suppressing alcohol absorption according to any one of claims 1 to 3, wherein the plant-derived peptide is a peptide derived from malt, tea or soybean.
5. The composition for suppressing alcohol absorption according to any one of claims 1 to 4, wherein the heat-treated product is a treated product at 100 ° C or higher and 0.101 MPa or higher.
6. 6. The alcohol absorption-suppressing composition according to any one of claims 1 to 5, which is used for suppressing an increase in blood alcohol concentration.
7. The composition for suppressing alcohol absorption according to any one of claims 1 to 6, which is used for treatment or prevention of a disease or symptom associated with an increase in blood alcohol concentration.
8. The composition for suppressing alcohol absorption according to any one of claims 1 to 7, which is labeled with a function exhibited by suppressing absorption of alcohol.
9. The function indications are “suppress alcohol absorption”, “moderate alcohol absorption”, “prevent hangover”, “friendly to the liver”, “protect the liver”, and “liver function” The composition for suppressing alcohol absorption according to claim 8, which is selected from the group consisting of “maintain”.
10. The composition for suppressing alcohol absorption according to any one of claims 1 to 9, wherein the composition is an agent.
11. Use of heat-treated products derived from plants to suppress alcohol absorption.
12. A method of suppressing alcohol absorption, using a plant-derived peptide heat-treated product as an active ingredient.

Images