WO2017000084A1 - Promethazine在制备抗肝癌和/或结肠癌和/或肺癌产品中的应用 - Google Patents

Promethazine在制备抗肝癌和/或结肠癌和/或肺癌产品中的应用 Download PDF

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WO2017000084A1
WO2017000084A1 PCT/CN2015/000479 CN2015000479W WO2017000084A1 WO 2017000084 A1 WO2017000084 A1 WO 2017000084A1 CN 2015000479 W CN2015000479 W CN 2015000479W WO 2017000084 A1 WO2017000084 A1 WO 2017000084A1
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cancer
promethazine
cell
drug
lung cancer
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PCT/CN2015/000479
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French (fr)
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师咏勇
宋智健
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上海交通大学
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Priority to PCT/CN2015/000479 priority Critical patent/WO2017000084A1/zh
Priority to US15/740,780 priority patent/US11197865B2/en
Publication of WO2017000084A1 publication Critical patent/WO2017000084A1/zh
Priority to US17/523,841 priority patent/US20220062292A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/54—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one sulfur as the ring hetero atoms, e.g. sulthiame
    • A61K31/5415—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one sulfur as the ring hetero atoms, e.g. sulthiame ortho- or peri-condensed with carbocyclic ring systems, e.g. phenothiazine, chlorpromazine, piroxicam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

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  • the invention relates to the field of biotechnology, and in particular to the use of Promethazine in the preparation of anti-liver cancer and/or colon cancer and/or lung cancer products.
  • Tumors are the most common and serious disease that threatens human health. Developing high-efficiency anti-tumor drugs is essential to prolong the life cycle of patients. In recent years, with the rapid development of tumor genomics and molecular pharmacology, the research and development of new anti-tumor drugs have achieved good results, but due to large investment in new drug research and development, long-term bottlenecks and other bottlenecks can not be overcome, as well as genetic variation of individual tumors. Characteristics, resulting in many traditional anti-tumor drugs are not effective, new drugs are expensive, side effects are unknown.
  • Nicardipine Promethazine, Estrone, Promethazine, Sunlidac, Prom ethazine, Etonogestrel, Levonorgestrel, Mesalazine, Indomethacin, Su Lfasalazine, Balsalazide, Irbesartan, Ibuprofen, Isoprenaline, Pento san Polysulfate.
  • Promethazine provided by the present invention for the preparation of a medicament for treating liver cancer and/or colon cancer and/or lung cancer.
  • a second object of the invention is to provide a new use of Promethazine.
  • the present invention provides the use of Promethazine for the preparation of a product for inhibiting proliferation of liver cancer and/or colon cancer and/or lung cancer cells.
  • a third object of the invention is to provide a new use of Promethazine.
  • the present invention provides the use of Promethazine in the preparation of a product that reduces the IC50 value of liver cancer and/or colon cancer and/or lung cancer cells.
  • Promethazine in the treatment of liver cancer and/or colon cancer and/or lung cancer is also within the scope of the present invention.
  • Promethazine in inhibiting the proliferation of liver cancer and/or colon cancer cells and/or lung cancer cells is also within the scope of the invention.
  • Promethazine as a medicament for the treatment of liver cancer and/or colon cancer and/or lung cancer is also within the scope of protection of the present invention.
  • Promethazine as a medicament for inhibiting proliferation of liver cancer and/or colon cancer cells and/or lung cancer cells is also within the scope of protection of the present invention.
  • the liver cancer cell is HUH-7; the colon cancer cell is LOVO, and the lung cancer cell is NCI-H2141.
  • the product is a drug or a kit.
  • a fourth object of the invention is to provide a product.
  • the product provided by the present invention has an active ingredient of Promethazine; the product has at least one of the following functions:
  • liver cancer and/or colon cancer and/or lung cancer 1) treating liver cancer and/or colon cancer and/or lung cancer;
  • the liver cancer cell is HUH-7; the colon cancer cell is LOVO, and the lung cancer cell is NCI-H2141.
  • the product is a drug or a kit.
  • Figure 1 shows the distribution of drug-coated tablets in a 96-well culture plate.
  • the drug to be tested in the following examples is Promethazine, and its chemical structural formula is as follows:
  • liver cancer cells HUH-7 and colon cancer cells LOVO liver cancer cells HUH-7 and colon cancer cells LOVO, human ovarian cancer cells SKOV-3 and large cell lung cancer cells NCI-H661 and lung cancer cells NCI-H2141 in the following examples are as follows:
  • Doxorubicin positive drug from MCE, Cat.No.HY-15142
  • Example 1 CELLTITER-GLO detection of Promethazine against liver cancer and/or colon cancer
  • HUH-7 cells and LOVO cells were subjected to the above methods to obtain HUH-796 well cell culture plates and LOVO 96 well cell culture plates, respectively.
  • HUH-7 cells the complete medium was DMEM, which was a life product, 11995081, and the cell density (cells/well) was 6000.
  • LOVO cells complete medium is DMEM
  • life product 11995081
  • cell density (cells/well) is 8000.
  • step b) Take 79 ⁇ L of the 20 mM drug to be tested, which was configured in step a), into the first well of the first row of the dilution plate, and then add 54 ⁇ L of DMSO solvent to the second to the ninth well of the first row, from the first well. Take 25 ⁇ L of the solution into the second well, mix and take 25 ⁇ L of the solution from the second well to the third well, and then push it to the ninth well, ensuring that the drug is successively subjected to a 3.16 fold dilution.
  • test drug and the positive drug loading template are shown in Figure 1, where S1208: positive drug Doxorubicin, DMSO: positive control well, Cpd 1,2,3: drug to be tested, final concentration of DMSO is 0.5% (DMSO) compatibility).
  • step 1 Take out step 1 from the incubator to prepare HUH-796 well cell culture plate and LOVO 96 well cell culture plate, and according to Figure 1, add the plate type arrangement (Fig. 1) to HUH-796 well cell culture plate and LOVO96 cell.
  • 10 ⁇ l of the cell culture medium (10X final concentration) containing the different concentrations of the drug prepared in the above b) was added to the culture plate, and cultured in a CO 2 incubator at 37 ° C for 72 hours to obtain a 96-well drug sieve plate of HUH-7 and 96 of LOVO. Porcelain sieve plate.
  • the final concentrations and dosing conditions of the above-mentioned drugs to be tested, the positive drug Doxorubicin and the control wells in a 96-well plate are as follows:
  • the final concentration ( ⁇ M) of the drug to be tested in the 2-10 well of FIG. 1 was: 100, 31.64557, 10.01442, 3.169112, 1.002886, 0.317369, 0.100433, 0.031783, 0.010058;
  • the final concentration ( ⁇ M) of the positive drug Doxorubicin in the 2-10 well of Figure 1 was: 30, 9.493371, 3.004326, 0.950736, 0.300866, 0.095211, 0.03013, 0.009535, 0.003017;
  • S1208 wells E1-H1 and A12-D12 in a 96-well plate: 10 ⁇ l of a final concentration of 100 ⁇ M
  • Doxorubicin solution solvent is a complete medium solution containing 0.5% DMSO
  • DMSO wells A1-D1, E12-H12 and A11 -H11: 10 ⁇ l of complete medium solution containing 0.5% DMSO.
  • NCI-H2141 cells the complete medium was HITES (DMEM: F12Medium), the life product, 11330057, and the cell density (cells/well) was 16,000.
  • Promethazine has a specific inhibitory effect on liver cancer and/or colon cancer and/or lung cancer cell proliferation, and can be used as a drug for treating liver cancer and/or colon cancer and/or lung cancer.
  • Table 1 shows the IC50 values of different cells under the action of Promethazine
  • the experiment of the present invention proves that the present invention performs tumor drug retargeting on the FDA and CFDA approved drug Promethazine, and screens for different anti-tumor drugs according to different cell lines (tissue types) and mutation sites of the tumor.
  • Promethazine is a new use of anti-liver cancer and/or colon cancer and/or lung cancer to achieve new use of old drugs.

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Abstract

本发明公开了Promethazine在制备治疗肝癌和/或结肠癌和/或肺癌产品中的应用，以及在制备抑制肝癌和/或结肠癌和/或肺癌细胞增殖产品中的应用；所述肝癌细胞为HUH-7，所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141；所述产品为药物或试剂盒。

Description

Promethazine在制备抗肝癌和/或结肠癌和/或肺癌产品中的应用 技术领域
本发明涉及生物技术领域，尤其涉及一种Promethazine在制备抗肝癌和/或结肠癌和/或肺癌产品中的应用。
背景技术
肿瘤是威胁人类健康的最常见也是最严重的一种疾病，研发高效抗肿瘤药物对延长患者的生存周期至关重要。近年来，随着肿瘤基因组学学和分子药理学的飞速发展，新型抗肿瘤药物的研发取得了不错的成果，但由于新药研发投入大，周期长等瓶颈无法克服，以及肿瘤个体遗传变异大等特征，导致许多传统抗肿瘤药物效果不加，新药价格昂贵，副作用未明。
Barabasi AL等研究者在2011年发表于《Nature Reviews Genetics》的论文中指出，基于GWAS研究结果和互作组学(interactome)策略开展的分子网络分析有望揭示复杂疾病新的药物靶点和分子标志物，并最终形成对疾病发病机制和治疗方案全新的认识。更值得注意的是，药物重定位(drug repositioning)研究发现，GWAS研究锁定的易感基因及与其有蛋白-蛋白相互作用(protein-protein interaction,PPI)的基因更容易成为药物间接的靶点，这一发现有助于解释现有药物的作用机制并指导新药的研发。2014年，Okada Y等研究者在《Nature》上发表的论文中显示GWAS研究结果整合分析得到的类风湿性关节炎的101个易感基因中有98个目前正用于治疗类风湿性关节炎药物的直接或间接靶标，而且还通过药物重定位研究，他们还发现了数十个已获批准用于其他适应症的药物也可用于治疗类风湿性关节炎。
发明公开
本研究正是通过整合癌症组学Cosmic version72数据库的癌症基因谱Cancer Gene Census以及蛋白质相互作用STRING version 10数据库和DrugBankversion4.2中FDA批准的药物数据库，获得的候选重定位药物，对肿瘤细胞系进行筛查实验，筛选出新的抗肿瘤药物。做肿瘤细胞系筛选的候选肿瘤抑制药物见如下:
Nicardipine,Promethazine,Estrone,Promethazine,Sunlidac,Prom ethazine,Etonogestrel,Levonorgestrel,Mesalazine,Indomethacin,Su  lfasalazine,Balsalazide,Irbesartan,Ibuprofen,Isoprenaline,Pento san Polysulfate。
本发明的一个目的是提供Promethazine的新用途。
本发明提供的Promethazine在制备治疗肝癌和/或结肠癌和/或肺癌产品中的应用。
本发明的第二个目的是提供Promethazine的新用途。
本发明提供了Promethazine在制备抑制肝癌和/或结肠癌和/或肺癌细胞增殖产品中的应用。
本发明的第三个目的是提供Promethazine的新用途。
本发明提供了Promethazine在制备降低肝癌和/或结肠癌和/或肺癌细胞IC50值产品中的应用。
Promethazine在治疗肝癌和/或结肠癌和/或肺癌中的应用也是本发明保护的范围；
或Promethazine在抑制肝癌和/或结肠癌细胞和/或肺癌细胞增殖中的应用也是本发明保护的范围。
Promethazine在作为治疗肝癌和/或结肠癌和/或肺癌药物中的应用也是本发明保护的范围；
或Promethazine在作为抑制肝癌和/或结肠癌细胞和/或肺癌细胞增殖药物中的应用也是本发明保护的范围。
上述应用中，所述肝癌细胞为HUH-7；所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141。
上述应用中，所述产品为药物或试剂盒。
本发明的第四个目的是提供一种产品。
本发明提供的产品，其活性成分为Promethazine；所述产品具有如下至少一种功能：
1)治疗肝癌和/或结肠癌和/或肺癌；
2)抑制肝癌和/或结肠癌和/或肺癌细胞增殖；
3)降低肝癌和/或结肠癌和/或肺癌细胞IC50值。
上述产品中，所述肝癌细胞为HUH-7；所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141。
上述产品中，所述产品为药物或试剂盒。
附图说明
图1为96孔培养板药筛药物板型分布。
图2为Promethazine对肝癌敏感；EC50＝41.9877；IC50＝31.3712；R2＝0.9988。
图3为Promethazine对结肠癌敏感；EC50＝18863738.7272；IC50＝33.4520；R2＝0.9164。
图4为Promethazine对肺癌敏感；EC50＝33.9362；IC50＝34.6324；R2＝0.9670。
实施发明的最佳方式
下述实施例中所使用的实验方法如无特殊说明，均为常规方法。
下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。
下述实施例中待测药物为Promethazine，其化学结构式如下:
Figure PCTCN2015000479-appb-000001
其为drug bank产品，产品目录号为DB01069。
下面实施例中的肝癌细胞HUH-7和结肠癌细胞LOVO、人卵巢癌细胞SKOV-3和大细胞肺癌细胞NCI-H661、肺癌细胞NCI-H2141的出处如下：
Figure PCTCN2015000479-appb-000002
下面实施例中的主要的仪器及耗材
DMSO(from Sigma,Cat.No.D4540)
96-well白色底透细胞培养板(from Corning,Cat.No.3610)
CellTiter Glo试剂盒(from Promega,Cat.No.G7573)
Doxorubicin阳性药(from MCE,Cat.No.HY-15142)
Fetal Bovine Serum(from Gibco,Cat#10099141)
100mm培养皿(from Corning,Cat#430167)
RPMI-1640medium(from Gibco,Cat#A1049101)
DMEM medium(from Gibco,Cat#11995081)
DMEM/F12medium(from Gibco,Cat#11330057)
EMEM medium(from Gibco,Cat#10370021)
Mutidrop 384细胞分液器(Thermo,Cat#5840150)
EnSpire多功能读板机(Perkin Elmer,Cat#2300-001M)
实施例1、CELLTITER-GLO检测Promethazine抗肝癌和/或结肠癌
一、待测孔板的制备
1、细胞铺板
a)配制各个细胞所需完全培养基。
b)实验开始前，确认标记在100mm培养皿上的药筛细胞名字，培养基以及传代时间，代次等信息，确保实验无误。
c)贴壁细胞操作参考步骤d)到i),悬浮细胞操作参考步骤j)到l)。
d)无菌操作时利用真空泵吸取细胞培养基。
e)用2ml的无菌PBS溶液润洗细胞表层，再用真空泵吸出PBS废液。
f)向培养皿轻轻加入1ml 0.25％(w/v)Trypsin-0.038％(w/v)EDTA溶液消化细胞，轻轻混匀几下，溶液覆盖住细胞表层，在倒置显微镜下观察细胞消化情况，在细胞将要脱落时终止胰酶消化作用。
g)向培养皿中加入5ml 37℃预热好的完全培养基，用移液管轻轻吹打细胞，使其从培养皿底部脱落下来。
h)将此细胞悬液转移到15ml或50ml无菌离心管中，1000rpm离心5min。
i)利用真空泵无菌操作吸出上清培养基。加入5ml 37℃预热好的完全培养基重悬细胞沉淀，轻轻吹打混匀。
j)用移液管轻轻吹打细胞，使其从培养皿底部完全脱落下来。
k)将此细胞悬液转移到15ml或50ml无菌离心管中，1000rpm离心5min。
l)利用真空泵无菌操作吸出上清培养基。加入5ml 37℃预热好的完全培养基重悬细胞沉淀，轻轻吹打混匀。
m)用细胞计数仪对细胞悬液进行计数，调整细胞悬液至合适密度接板进行细胞铺板实验。
n)将HUH-7细胞和LOVO细胞按照上述方法进行，分别得到HUH-796孔细胞培养板和LOVO96孔细胞培养板。
HUH-7细胞，完全培养基为DMEM，为life产品，11995081，接板密度(cells/well)为6000。
LOVO细胞，完全培养基为DMEM，为life产品，11995081，接板密度(cells/well)为8000。
2、待测药物Promethazine配制和加药(200X终浓度):
1)待测药物Promethazine母板配制
a)用DMSO将待测药物Promethazine稀释到20mM待用。
b)取a)步骤中配置好的20mM待测药物79μL加入稀释板第一行第一孔中，随后加入54μL的DMSO溶剂到第一行第二孔至第九孔中，从第一孔中取25μL溶液到第二孔中，混匀后从第二孔中取25μL溶液到第三孔中，依次类推到第九孔，保证药物逐次进行3.16倍倍比梯度稀释。
2)阳性药Doxorubicin(MCE,Cat.No.HY-15142)母板配制
a)用DMSO将阳性药Doxorubicin稀释到6mM待用。
b)将6mM阳性药Doxorubicin溶液加入稀释板中，DMSO溶液1:3.16倍倍比梯度稀释该待测药物。
3、药物工作板配制及加药
a)待测药物及阳性药加样模板如下图1所示，其中，S1208:阳性药Doxorubicin,DMSO:阳性对照孔,Cpd 1,2,3:待测药物,DMSO终浓度为0.5％(DMSO兼容性)。
b)向工作板中加入95μl细胞特定的完全培养基，每个药物对应9个孔，用多道排枪从待测药物和阳性药doxorubicin母板中分别依次转移5μl一系列(9个孔)倍比稀释好的溶液(10X终浓度)，得到含有不同浓度药物的细胞培养基。
c)从培养箱中取出步骤1制备HUH-796孔细胞培养板和LOVO96孔细胞培养板，按图1加药板型排列方式(图1)分别向HUH-796孔细胞培养板和LOVO96孔细胞培养板中加入10μl上述b)制备的含有不同浓度药物的细胞培养基(10X终浓度)，放入到CO2培养箱37℃培养72h，得到HUH-7的96孔药筛板和LOVO的96孔药筛板。
以不加任何药物的作为对照孔。
上述待测药物、阳性药Doxorubicin和对照孔在96孔板中的终浓度及加药情况如下：
待测药物在图1的2-10孔中的终浓度(μM)依次为:100、31.64557、10.01442、3.16912、1.002886、0.317369、0.100433、0.031783、0.010058；
阳性药Doxorubicin在图1的2-10孔中的终浓度(μM)依次为:30、9.493671、3.004326、0.950736、0.300866、0.095211、0.03013、0.009535、0.003017；
此外，96孔板中S1208孔(E1-H1和A12-D12):10μl终浓度100μM Doxorubicin溶液(溶剂为包含0.5％DMSO的完全培养基溶液),DMSO孔(A1-D1,E12-H12和A11-H11):10μl包含0.5％DMSO的完全培养基溶液。
二、CELLTITER-GLO荧光细胞活性检测系统检测
1、CellTiter-Glo试剂配制
a)使用前，将CellTiter-Glo试剂Buffer融化，平衡到室温使用。
b)使用前，将CellTiter-Glo试剂冻粉底物融化，平衡到室温使用。
c)取100ml平衡好的CellTiter-Glo Buffer加入到瓶装CellTiter-Glo试剂冻粉底物中，使其充分重悬形成酶/底物混合物，也就是所谓的CellTiter-Glo检测试剂。
d)轻轻混匀涡旋，并反复倒置获得均匀的溶液。一般来说，1分钟内CellTiter-Glo底物试剂就会充分溶解，分装，避光保存在-20℃备用，避免反复冻融。
2、检测
a)检测前，将上述一的3得到的HUH-7的96孔药筛板和LOVO的96孔药筛板在室温中平衡20-30分钟。
b)倒置显微镜观察每块培养板的细胞形态及死亡情况，标记出异常情况并复测一次。
c)向所有药筛孔中加入100μl上述1制备的CellTiter-Glo试剂，混匀。
d)在96孔微孔板振荡器上充分震荡混匀2分钟，使细胞完全裂解。
e)避光室温放置15分钟后进行冷光信号检测，保证信号的稳定性。
f)使用EnSpire多功能读板机570nm时读取冷光信号。
g)分析处理数据。
HUH-7的96孔药筛板结果如图2所示。
LOVO的96孔药筛板结果如图3所示。
计算IC50值，结果如表1所示。
采用同样的方法检测Promethazine作用人卵巢癌细胞SKOV-3和大细胞肺癌细胞NCI-H661，细胞的IC50值，结果如表1。
采用同样的方法检测Promethazine作用肺癌细胞，细胞的IC50值，结果如表1和图4。
NCI-H2141细胞，完全培养基为HITES(DMEM:F12Medium)，为life产品，11330057，接板密度(cells/well)为16000。
可以看出，Promethazine对肝癌和/或结肠癌和/或肺癌细胞增殖具有特异抑制作用，可以用来作为治疗肝癌和/或结肠癌和/或肺癌的药物。
表1为不同细胞在Promethazine药物作用下的IC50值
Figure PCTCN2015000479-appb-000003
Figure PCTCN2015000479-appb-000004
工业应用
本发明的实验证明，本发明对FDA,CFDA已获批准的药物Promethazine进行肿瘤药物重定位，根据肿瘤不同的细胞系(组织类型)以及突变位点对适应症不是抗肿瘤的药物进行筛选，发现Promethazine抗肝癌和/或结肠癌和/或肺癌这种新用途，实现旧药新用。

Claims (9)

  1. Promethazine在制备治疗肝癌和/或结肠癌和/或肺癌产品中的应用；
    或Promethazine在制备抑制肝癌和/或结肠癌细胞和/或肺癌细胞增殖产品中的应用。
  2. 根据权利要求1所述的应用，其特征在于：所述肝癌细胞为HUH-7；所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141；所述产品为药物或试剂盒。
  3. Promethazine在治疗肝癌和/或结肠癌和/或肺癌中的应用；
    或Promethazine在抑制肝癌和/或结肠癌细胞和/或肺癌细胞增殖中的应用。
  4. 根据权利要求3所述的应用，其特征在于：所述肝癌细胞为HUH-7；所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141。
  5. Promethazine在作为治疗肝癌和/或结肠癌和/或肺癌药物中的应用；
    或Promethazine在作为抑制肝癌和/或结肠癌细胞和/或肺癌细胞增殖药物中的应用。
  6. 根据权利要求5所述的应用，其特征在于：所述肝癌细胞为HUH-7；所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141。
  7. 一种产品，其活性成分为Promethazine；所述产品具有如下1)和/或2)功能：
    1)治疗肝癌和/或结肠癌和/或肺癌；
    2)抑制肝癌和/或结肠癌和/或肺癌细胞增殖。
  8. 根据权利要求7所述的产品，其特征在于：所述肝癌细胞为HUH-7；所述结肠癌细胞为LOVO，所述肺癌细胞为NCI-H2141。
  9. 根据权利要求7所述的产品，其特征在于：所述产品为药物或试剂盒。
PCT/CN2015/000479 2015-06-30 2015-06-30 Promethazine在制备抗肝癌和/或结肠癌和/或肺癌产品中的应用 WO2017000084A1 (zh)

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US15/740,780 US11197865B2 (en) 2015-06-30 2015-06-30 Applications for promethazine in preparing anti-liver cancer and/or colon cancer and/or lung cancer products
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CN1878556A (zh) * 2003-09-18 2006-12-13 康宾纳特克斯公司 治疗肿瘤的联合药物

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