WO2016176921A1 - 用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法及获得的网片材料 - Google Patents

用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法及获得的网片材料 Download PDF

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WO2016176921A1
WO2016176921A1 PCT/CN2015/086357 CN2015086357W WO2016176921A1 WO 2016176921 A1 WO2016176921 A1 WO 2016176921A1 CN 2015086357 W CN2015086357 W CN 2015086357W WO 2016176921 A1 WO2016176921 A1 WO 2016176921A1
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polydopamine
polypropylene
polypropylene mesh
abdominal wall
mesh
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PCT/CN2015/086357
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English (en)
French (fr)
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王辉
陈双
吴丁财
黄榕康
胡民辉
杨科力
赵日升
曾杨
赵洁
梁业如
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王辉
陈双
吴丁财
黄榕康
胡民辉
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Publication of WO2016176921A1 publication Critical patent/WO2016176921A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/10—Macromolecular materials

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  • the invention relates to the technical field of medical instruments and preparation thereof, in particular to a preparation method of a polydopamine-coated anti-adhesion polypropylene mesh for repairing abdominal wall defects, and a method for repairing abdominal wall defects obtained by the preparation method Dopamine coated anti-adhesive polypropylene mesh material.
  • Polypropylene is a traditional repair material for abdominal wall defects. Since Usher used polypropylene mesh for inguinal hernia repair in 1963, tension-free repair of abdominal defects has become a common procedure in surgery, with tens of thousands of surgical patches per day for the repair of abdominal wall defects.
  • abdominal wall defect repair can only place the polypropylene mesh in the peritoneum, and can not directly contact the abdominal internal organs. This is because the polypropylene mesh and the intra-abdominal organs can cause serious adhesion, and even lead to perforation of the digestive tract, causing serious complication.
  • the solution to this scientific problem is to combine polyacrylic acid, animal gelatin and other substances that can be catabolized in the body to achieve anti-adhesion.
  • these materials require more preparation, complicated processes, and prices. It is high and has certain timeliness. Therefore, it is of great significance to develop a simple, effective, and a certain tensile strength of the abdominal anti-adhesive patch.
  • a second object of the present invention is to provide a polydopamine-coated anti-adhesion polypropylene mesh material for repairing abdominal wall defects obtained by the above preparation method, which has better biocompatibility and better Anti-adhesion and tolerance to infection.
  • the technical solution provided by the invention is: a preparation method of a polydopamine-coated anti-blocking polypropylene mesh for repairing abdominal wall defects, comprising the following steps:
  • the preparation method is preferably: preparing a polypropylene mesh sheet, and placing the polypropylene mesh sheet into 6-11 mmol/L trishydroxymethylamino group.
  • a certain amount of dopamine hydrochloride is added to prepare a dopamine hydrochloride solubility of 1.6-2.4 g/L, and the reaction is stirred under an air atmosphere of 25-30 ° C for 24-48 hours, taken out, rinsed and dried to obtain a polydopamine coating.
  • Anti-blocking polypropylene mesh material is preferably: preparing a polypropylene mesh sheet, and placing the polypropylene mesh sheet into 6-11 mmol/L trishydroxymethylamino group.
  • a certain amount of dopamine hydrochloride is added to prepare a dopamine hydrochloride solubility of 1.6-2.4 g/L, and the reaction is stirred under an air atmosphere of 25-30 ° C for 24-48 hours, taken out, rinsed and dried to obtain a polydopamine coating
  • the stirring reaction is performed at a rotational speed of 100 to 1000 rpm, that is, 100 to 1000 rpm.
  • the polypropylene mesh sheet is a medical lightweight patch woven from polypropylene fibers.
  • the polypropylene mesh has a mesh diameter of between 2 mm and 4 mm.
  • the surface of the polydopamine-coated anti-adhesion polypropylene mesh material is a polydopamine film
  • the polydopamine film has a thickness of 500 nm to 5 ⁇ m.
  • the present invention also provides a polydopamine-coated anti-adhesion polypropylene mesh material obtained by the above-mentioned preparation method for repairing abdominal wall defects, comprising a polypropylene mesh sheet and a polydopamine film, which are aggregated in the polypropylene mesh sheet.
  • the surface of the propylene material is coated with a polydopamine film to form a polypropylene/polydopamine composite mesh material, specifically, dopamine is polymerized in situ on the surface of the polypropylene patch to form a layer of polydopamine film.
  • the polydopamine film has a thickness of 500 nm to 5 ⁇ m.
  • the polypropylene mesh sheet is a medical lightweight patch woven from polypropylene filament, and a mesh of polypropylene mesh.
  • the hole diameter is 2mm-4mm.
  • the polypropylene mesh sheet can also be prepared in other manners, such as a polypropylene mesh sheet obtained by an integral molding technique and a polypropylene mesh sheet obtained by extrusion, and the present invention is not limited.
  • the invention has the following beneficial effects:
  • the polydopamine-coated anti-adhesion polypropylene mesh material obtained for the repair of abdominal wall defects obtained by the preparation method of the present invention has a simple manufacturing process, low cost and stable properties.
  • the polydopamine of the present invention is a dopamine macromolecular polymer, has stable properties, has good hydrophilicity and biocompatibility; and is stably attached to the surface of the polypropylene material by complicated physical and chemical action.
  • the formation of a layer of polydopamine film, so that the polypropylene material does not directly contact the abdominal organs, in theory, can greatly reduce the complications such as intestinal adhesion caused by polypropylene contact with abdominal organs, animal experiments have also been initially confirmed.
  • polydopamine is a hydrophilic and biocompatible macromolecule that can be self-polymerized on the surface of a polypropylene material by dopamine oxidation and is stable in nature.
  • the structure can establish covalent and non-covalent interactions with the surface of the polypropylene material, and the adhesion is coated on the surface of the polypropylene material by complex physicochemical interaction to form a layer.
  • Polydopamine film The invention polymerizes dopamine in situ on the surface of the polypropylene patch material to form a biocompatible polydopamine film, so that the polypropylene patch does not directly contact the abdominal cavity organ, and the intraperitoneal repair of the abdominal wall defect can greatly reduce the intestinal tract. The incidence of complications such as adhesions, intestinal obstruction, intestinal fistula and infection.
  • the polydopamine-coated anti-adhesive polypropylene mesh material obtained for repairing abdominal wall defects of the present invention still maintains a certain mesh structure, which facilitates tissue growth, free entry and exit of macrophages and white blood cells, and has a comparative Good tissue strength, tensile strength and anti-infective properties.
  • the present invention contemplates a polydopamine-coated anti-adhesive polypropylene mesh material for repair of abdominal wall defects, which is a polypropylene-woven mesh having a surface coated with a polydopamine film.
  • the polydopamine is stable in nature and does not decompose into a biologically active dopamine monomer. It is a high molecular polymer which cannot be absorbed and utilized by a living body; we coat it on the surface of a polypropylene mesh sheet to produce an anti-blocking effect.
  • Implanted into the peritoneal cavity as a material for repairing the abdominal wall defect it has the function of strengthening the abdominal wall tension, anti-adhesion, and tolerating infection.
  • the polydopamine-coated anti-adhesive polypropylene mesh obtained for the repair of abdominal wall defect obtained by the preparation method of the invention has good mechanical properties, good biocompatibility, better anti-blocking property and better Tolerate infection.
  • Embodiment 1 is a schematic structural view of Embodiment 1 of the present invention.
  • Figure 2 is a partial cross-sectional view showing Embodiment 1 of the present invention.
  • Figure 3 is a scanning electron micrograph of a polypropylene mesh sheet according to Embodiment 1 of the present invention.
  • Figure 4 is a scanning electron micrograph of the polydopamine-coated anti-blocking polypropylene mesh material of Example 1 of the present invention.
  • Figure 5 is a scanning electron micrograph of a dopamine film of a polydopamine-coated anti-blocking polypropylene mesh material of Example 1 of the present invention
  • Figure 6 is an enlarged view of a portion A of a scanning electron micrograph of a dopamine film of a polydopamine-coated anti-blocking polypropylene mesh material of Example 1 of the present invention
  • Figure 7 is a comparison view of a polypropylene mesh sheet and a polydopamine-coated anti-blocking polypropylene mesh material of Example 1 of the present invention
  • Fig. 8 is a view showing the effect of the second embodiment of the present invention applied to a rat.
  • Three pieces of 3cm ⁇ 3cm polypropylene mesh were placed in a Trihydroxyl buffer solution with a concentration of 9mmol/L.
  • the surface of the polypropylene material was smooth by electron microscopy.
  • the polypropylene mesh was woven with polypropylene.
  • the medical lightweight patch has a pore size of 2mm, the surface of the polypropylene material is smooth, no dopamine and other substances are attached; then 0.9g of dopamine hydrochloride is added, and the final concentration of dopamine hydrochloride is 1.8g/L, and the air is at 28°C.
  • the stirring reaction was carried out under an atmosphere at a rotation speed of 400 rpm, and the reaction was taken out after 24 hours.
  • the resulting polypropylene mesh was rinsed with a large amount of deionized water until clarified, and dried in a vacuum oven at 30 ° C to obtain a black polydopamine-coated anti-blocking polypropylene mesh material for repair of abdominal wall defects.
  • 1 is an unreacted and treated polypropylene mesh sheet
  • 2 is a polydopamine-coated anti-blocking polypropylene mesh material obtained by the reaction of Example 1.
  • the thickness of the polydopamine film was determined to be about 4.8 ⁇ m by electron microscopic observation of the polypropylene mesh sheet as shown in Fig. 3 and the black polydopamine-coated anti-blocking polypropylene mesh material, as shown in Fig. 4 and Fig. 5 It can be seen that polydopamine nanospheres are visible on the surface of the polypropylene. As can be clearly seen in Fig. 6, visible polydopamine nanospheres are coated on the surface of the polypropylene.
  • FIG. 1 is a schematic structural view of the present embodiment
  • FIG. 2 is a partial cross-sectional view of FIG. 1, wherein 10 is a black anti-adhesive polypropylene mesh material for polydopamine coating obtained in the present embodiment, including polypropylene.
  • the mesh 11 and the polydopamine film 12 are specifically shown in Fig. 2, and the structure can also be clearly and intuitively observed from the electron micrograph of Fig. 4.
  • the polydopamine film 12 is a film formed by in-situ polymerization of polydopamine on the surface of the polypropylene material of the polypropylene mesh sheet 11, and the thickness of the polydopamine film 12 varies depending on the preparation process, and is in situ by dopamine.
  • the preparation of the polypropylene/polydopamine repair material by polymerization can improve the composite stability of the polydopamine membrane 12, facilitate the chemical and biological stability during the long-term contact with the human biological tissue, and improve the biocompatibility.
  • the risk of infection is reduced, and long-term use due to its stability characteristics can ensure the long-lasting isolation of the polypropylene mesh sheet 12 and the biological tissue, preventing adhesion.
  • Two pieces of 2 cm ⁇ 3 cm polypropylene mesh sheets were placed in a 50 ml buffer solution of trimethylolamine (Tris) at a concentration of 10 mmol/L, which was a medical lightweight patch woven from polypropylene.
  • Tris trimethylolamine
  • the pore size was 2.5 mm, and then 1 g of dopamine hydrochloride was added, and the final concentration of dopamine hydrochloride was 2 g/L, and the reaction was stirred at 30 ° C, the rotation speed was 600 rpm, and the reaction was taken out after 48 hours.
  • the resulting polypropylene mesh was rinsed with a large amount of deionized water until clarified, and dried in a vacuum oven at 30 ° C to obtain a dark black polydopamine-coated anti-blocking polypropylene mesh material.
  • the thickness of the polydopamine film was determined to be about 1.5 ⁇ m by electron microscopic observation of the dark black polypropylene/polydopamine repair material.
  • the polydopamine film thickness was approximately 800 nm by electron microscopic observation of a light black polypropylene/polydopamine repair material.
  • a piece of 4 cm ⁇ 3 cm polypropylene mesh was placed in a 100 ml buffer solution of Trimmol in a concentration of 6 mmol/L.
  • the polypropylene mesh was a medical lightweight patch woven from polypropylene.
  • the pore size was 4 mm, and then 1.6 g of dopamine hydrochloride was added, and the final concentration of dopamine hydrochloride was 1.6 g/L.
  • the stirring reaction was carried out at 30 ° C, the rotation speed was 800 rpm, and the reaction was taken out after 24 hours.
  • the resulting polypropylene mesh was rinsed with a large amount of deionized water until clarified, and dried in a vacuum oven at 45 ° C to obtain a light black polydopamine-coated anti-block polypropylene mesh material.
  • the polydopamine film thickness was about 1.0 ⁇ m by electron microscopic observation of a light black polypropylene/polydopamine repair material.
  • the ethylene oxide-sterilized polydopamine-coated anti-blocking polypropylene mesh material is prepared, and the preparation method is as in Example 2, the size is 2 cm ⁇ 3 cm, and it is gently placed in the abdominal cavity, and the patch is well-angled with a silk thread. Stitching is fixed on the abdominal wall, and the abdominal cavity is closed layer by layer. Subsequently, the rats' mental and dietary conditions were observed. After 10 days, the abdominal cavity was opened to observe whether the patch had adhesion and the severity of adhesion.

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Abstract

一种用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片(10)及其制备方法，所述网片(10)以聚丙烯编织的网片(11)为骨架，表面包被有聚多巴胺膜(12)。所述制备方法为：将聚丙烯网片(11)放入三羟甲基氨基甲烷溶液中，再加入盐酸多巴胺，通过条件反应，得到聚多巴胺膜(12)包被的防粘连聚丙烯网片(10)。

Description

用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法及获得的网片材料 技术领域
本发明涉及医疗器械及其制备技术领域，特别是一种用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，以及该制备方法所获得的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料。
背景技术
聚丙烯是传统的腹壁缺损修补材料。自1963年Usher把聚丙烯网片用于腹股沟疝修补以来，腹部缺损的无张力修补已经成为外科的一种常见操作，每天有数以万计的外科补片用于腹壁缺损的修补。
目前，腹壁缺损修补只能将聚丙烯网片置于腹膜外，不能直接接触腹腔内脏器，这是由于聚丙烯网片与腹腔内器官接触可产生严重粘连，甚至导致消化道穿孔，引发严重的并发症。现阶段，解决这一科学问题的方法是将聚丙烯材料复合聚乳酸、动物明胶等在机体内可以分解代谢的物质，才能达到防粘连作用，但这些材料的制备要求较多，工艺繁杂，价格高昂，且有一定的时效性，因此开发一种简单、有效，且具有一定抗张强度的腹外疝防粘连修补网片具有重大意义。
发明内容
本发明的一个目的是提供一种用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料的制备方法。
本发明的第二个目的提供一种采用上述制备方法所获得的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，该材料具有较好的生物相容性、更好地防粘连性和耐受感染的作用。
本发明提供的技术方案为：一种用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，包括以下步骤：
准备聚丙烯网片，将聚丙烯网片放入5-20mmol/L三羟甲基氨基甲烷溶液中，随后加入一定量的盐酸多巴胺，配制盐酸多巴胺溶度为0.5-2.8g/L，在10-40℃空气氛围下搅拌反应10-72h后取出，冲洗并干燥，得到聚多巴胺包被的防粘连聚丙烯网片材料。
所述的制备方法优选为：准备聚丙烯网片，将聚丙烯网片放入6-11mmol/L三羟甲基氨基 甲烷溶液中，随后加入一定量的盐酸多巴胺，配制盐酸多巴胺溶度为1.6-2.4g/L，在25-30℃空气氛围下搅拌反应24-48h后取出，冲洗并干燥，得到聚多巴胺包被的防粘连聚丙烯网片材料。
在上述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法中，搅拌反应的转速为100-1000rpm，即100-1000转/每分钟。
在上述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法中，所述的聚丙烯网片是聚丙烯丝编织而成的医用轻量补片
在上述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法中，所述的聚丙烯网片的网孔直径为2mm-4mm之间。
在上述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法中，所述的聚多巴胺包被的防粘连聚丙烯网片材料的表面为聚多巴胺膜，所述的聚多巴胺膜的厚度为500nm-5μm。
本发明还提供通过上述的制备方法所得到的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，包括聚丙烯网片和聚多巴胺膜，在所述聚丙烯网片的聚丙烯材料表面包被有聚多巴胺膜而形成聚丙烯/聚多巴胺复合网片材料，具体来说为多巴胺在聚丙烯补片表面原位聚合，形成一层聚多巴胺膜。
在上述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料中，所述的聚多巴胺膜的厚度为500nm-5μm。
在上述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料中，所述的聚丙烯网片是聚丙烯丝编织而成的医用轻量补片，聚丙烯网片的网孔直径为2mm-4mm。当然，聚丙烯网片还可以采用其他的方式制备得到，如采用一体成型技术得到的聚丙烯网片，通过挤出方式得到的聚丙烯网片，对此本发明不作过多的限制。
本发明在采用上述技术方案后，其具有的有益效果为：
(1)本发明的制备方法所获得的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，制作工艺简单，成本低，性质稳定。
(2)本发明所述的聚多巴胺作为一种多巴胺大分子聚合物，性质稳定，具有良好的亲水性及生物相容性；通过复杂的物理化学作用稳定附着包被在聚丙烯材料表面，形成一层聚多巴胺膜，使聚丙烯材料不直接接触腹腔脏器，在理论上可极大的降低由聚丙烯接触腹腔脏器说引起的肠粘连等并发症，动物实验也已初步证实。具体来说，聚多巴胺是一种亲水性及生物相容性的大分子，可通过多巴胺氧化自聚在聚丙烯材料的表面，性质稳定。其作用机理来 自于多巴胺的邻苯二酚与氨基官能团，该结构能与聚丙烯材料表面建立共价和非共价的相互作用，通过复杂的物理化学作用稳定附着包被在聚丙烯材料表面，形成一层聚多巴胺膜。本发明在聚丙烯补片材料表面原位聚合多巴胺形成一层生物相容性较好的聚多巴胺膜，使聚丙烯补片不直接接触腹腔脏器，应用于腹壁缺损腹膜内修补可大幅降低肠粘连、肠梗阻、肠瘘及感染等并发症的发生率。
(3)本发明所得的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料仍然保持有一定的网孔结构，利于组织长入，巨噬细胞和白细胞的自由进出，具有较好的组织强度、抗拉性以及抗感染性。
总之，本发明设计一种用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，该材料以聚丙烯编织的网片为骨架，其表面包被有聚多巴胺膜。该聚多巴胺性质稳定，不分解为具有生物活性的多巴胺单体，是一种不能被生物体吸收利用的高分子聚合物；我们将其包被在聚丙烯网片表面，可产生防粘连作用，植入腹膜腔内作为腹壁缺损修补的材料，具有加强腹壁张力、防粘连、耐受感染的作用，可安全的放置于腹膜腔内，是一种全新的医用腹外疝修复材料。本发明的制备方法所获得的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片具有良好的力学性能、较好的生物相容性、更好地防粘连性及可较好的耐受感染。
附图说明
图1是本发明实施例1的结构示意图；
图2是本发明实施例1的局部剖视图；
图3是本发明实施例1的聚丙烯网片的扫描电镜图；
图4是本发明实施例1的聚多巴胺包被的防粘连聚丙烯网片材料的扫描电镜图；
图5是本发明实施例1的聚多巴胺包被的防粘连聚丙烯网片材料的多巴胺膜扫描电镜图；
图6是本发明实施例1的聚多巴胺包被的防粘连聚丙烯网片材料的多巴胺膜扫描电镜图的局部A的放大图；
图7是本发明实施例1的聚丙烯网片和聚多巴胺包被的防粘连聚丙烯网片材料的对比图；
图8是本发明实施例2的应用于大鼠时的效果图。
具体实施方式
下面结合具体实施方式，对本发明的技术方案作进一步的详细说明，但不构成对本发明的任何限制。
实施例1：
将3片3cm×3cm的聚丙烯网片放入浓度为9mmol/L的三羟甲基氨基甲烷(Tris)缓冲溶液中，电镜观察聚丙烯材料表面光滑，该聚丙烯网片为聚丙烯编织而成的医用轻量补片，孔径为2mm，聚丙烯材料表面光滑，未附着多巴胺及其它物质；随即加入0.9g盐酸多巴胺，配制成的盐酸多巴胺的最终浓度为1.8g/L，在28℃空气氛围下下进行搅拌反应，转速为400rpm，反应24h后取出。所得的聚丙烯网片用大量去离子水冲洗溶液至澄清，在30℃真空干燥箱中烘干，得到黑色的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料。
如附图7所示：1为未经反应和处理的聚丙烯网片，2为经过实施例1的反应得到的聚多巴胺包被的防粘连聚丙烯网片材料。
通过分别对聚丙烯网片如图3和黑色的聚多巴胺包被的防粘连聚丙烯网片材料如图4进行电镜观察，测得聚多巴胺膜厚度约为4.8μm，如图4和图5所示，聚丙烯表面可见聚多巴胺纳米球，图6可以清楚的看到，在聚丙烯表面包被有可见聚多巴胺纳米球。
图1示出了本实施例的结构示意图，图2为图1的局部剖视图，其中，10为本实施例得到的黑色的用于聚多巴胺包被的防粘连聚丙烯网片材料，包括聚丙烯网片11和聚多巴胺膜12，具体如图2所示，此外从图4的电镜图上也可以清楚直观的观察到该结构。聚多巴胺膜12是聚多巴胺以原位聚合的方式在聚丙烯网片11的聚丙烯材料的表面的而形成的薄膜，根据制备工艺的变化聚多巴胺膜12的厚度有所不同，通过多巴胺原位聚合的方式制备本聚丙烯/聚多巴胺修复材料可以提高聚多巴胺膜12的复合稳定性，有利于在长时间的和人体生物组织接触过程中的化学和生物稳定性，提高了生物相容性，降低了感染风险，并且长时间使用由于其稳定性的特点，能够保证聚丙烯网片12和生物组织持久性的隔离，防止粘连。
实施例2：
将2片2cm×3cm的聚丙烯网片放入浓度为10mmol/L的三羟甲基氨基甲烷(Tris)50ml缓冲溶液中，该聚丙烯网片为聚丙烯编织而成的医用轻量补片，孔径为2.5mm，随即加入1g盐酸多巴胺，配制成的盐酸多巴胺的最终浓度为2g/L，在30℃下进行搅拌反应，转速为600rpm，反应48h后取出。所得的聚丙烯网片用大量去离子水冲洗溶液至澄清，在30℃真空干燥箱中烘干，得到深黑色的聚多巴胺包被的防粘连聚丙烯网片材料。
通过对深黑色的聚丙烯/聚多巴胺修复材料进行电镜观察测得聚多巴胺膜厚度约为1.5μm。
实施例3
将4片2cm×2cm的聚丙烯网片放入浓度为11mmol/L的三羟甲基氨基甲烷(Tris)50ml缓冲溶液中，该聚丙烯网片为聚丙烯编织而成的医用轻量补片，孔径为3mm，随即加入1.2g盐酸多巴胺，配制成的盐酸多巴胺的最终浓度为2.4g/L，在25℃下进行搅拌反应，转速为600rpm，反应36h后取出。所得的聚丙烯网片用大量去离子水冲洗溶液至澄清，在30℃真空干燥箱中烘干，得到浅黑色的聚多巴胺包被的防粘连聚丙烯网片材料。
通过对浅黑色的聚丙烯/聚多巴胺修复材料进行电镜观察聚多巴胺膜厚度约为800nm。
实施例4：
将1片4cm×3cm的聚丙烯网片放入浓度为6mmol/L的三羟甲基氨基甲烷(Tris)100ml缓冲溶液中，该聚丙烯网片为聚丙烯编织而成的医用轻量补片，孔径为4mm，随即加入1.6g盐酸多巴胺，配制成的盐酸多巴胺的最终浓度为1.6g/L，在30℃下进行搅拌反应，转速为800rpm，反应24h后取出。所得的聚丙烯网片用大量去离子水冲洗溶液至澄清，在45℃真空干燥箱中烘干，得到浅黑色的聚多巴胺包被的防粘连聚丙烯网片材料。
通过对浅黑色的聚丙烯/聚多巴胺修复材料进行电镜观察聚多巴胺膜厚度约为1.0μm。
动物实施例1
取3只重约300g-350g雄性Wistar大鼠，用1％戊巴比妥钠对大鼠进行腹腔注射麻醉，用量为50ml/kg，麻醉诱导时间为8min。随即暴露大鼠腹部，用剪刀在腹中央清理出一块8cm×8cm无毛区域。常规消毒铺巾，用手术刀在大鼠腹部切开两条长约3.5cm皮肤切口，切口间隔3cm，切口平行大鼠躯干纵轴，随后沿切口逐层分离并切开腹部肌肉各层，进入腹腔。
准备好环氧乙烷消毒的聚多巴胺包被的防粘连聚丙烯网片材料，其制备方法如实施例2，大小为2cm×3cm，轻柔的放入腹腔内，用丝线将补片四角良好的缝合固定在腹壁，逐层关闭腹腔。随后观察大鼠精神、饮食情况，10天后打开腹腔，观察补片有无粘连及粘连严重程度。
10天后，打开3只大鼠腹腔，腹腔均未见感染，无包裹性积液及积脓，补片均未见明显粘连，可见补片接触腹腔的一面已基本腹膜化，如图8所示，未见肠管粘连。
上述实施例为本发明较佳的实施方式，但本发明的实施方式并不受上述实施例的限制，其它的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化，均应 为等效的置换方式，都包含在本发明的保护范围之内。

Claims (9)

  1. 一种用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，其特征在于,所述的制备方法为：
    准备聚丙烯网片，将聚丙烯网片放入5-20mmol/L三羟甲基氨基甲烷溶液中，随后加入一定量的盐酸多巴胺，配制盐酸多巴胺溶度为0.5-2.8g/L，在10-40℃的空气氛围下搅拌反应10-72h后取出，冲洗并干燥，得到聚多巴胺包被的防粘连聚丙烯网片。
  2. 根据权利要求1所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，其特征在于：搅拌反应的转速为100-1000rpm。
  3. 根据权利要求1所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，其特征在于：所述的聚丙烯网片是聚丙烯丝编织而成的医用轻量补片。
  4. 根据权利要求3所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，其特征在于：所述的聚丙烯网片的网孔直径为2mm-4mm之间。
  5. 根据权利要求1所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片的制备方法，其特征在于：所述的聚多巴胺包被的防粘连聚丙烯网片的表面为聚多巴胺膜，所述的聚多巴胺膜的厚度为500nm-5μm。
  6. 一种采用权利要求1所述的方法获得的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，其特征在于：包括聚丙烯网片和聚多巴胺膜，在所述聚丙烯网片的聚丙烯材料表面包被有聚多巴胺膜而形成聚多巴胺包被的防粘连聚丙烯网片材料。
  7. 根据权利要求6所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，其特征在于：所述的聚多巴胺膜的厚度为500nm-5μm。
  8. 根据权利要求6所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，其特征在于：所述的聚丙烯网片是聚丙烯丝编织而成的医用轻量补片，所述聚多巴胺膜包裹在聚丙烯丝表面。
  9. 根据权利要求8所述的用于腹壁缺损修补的聚多巴胺包被的防粘连聚丙烯网片材料，其特征在于：所述的聚丙烯网片的网孔直径为2mm-4mm之间。
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